Cleaning compositions and uses thereof

Abstract

This invention relates to cleaning compositions and their uses. Compositions, such as cleaning compositions comprising enzyme mixtures, are provided. The invention further relates to the use of compositions comprising such enzymes in cleaning processes and / or for deep cleaning of organic dirt, and methods for removing or reducing components of organic matter.

Description

[0001] This application is a divisional application of Chinese patent application No. 201880026886.3, filed on April 6, 2018, entitled "Cleaning Composition and Use Thereof".

[0002] References to sequence lists

[0003] This application contains a sequence list in computer-readable form, which is incorporated herein by reference. Background of the Invention

[0005] This invention relates to compositions, such as cleaning compositions comprising enzyme mixtures. The invention further relates to the use of compositions comprising such enzymes in cleaning processes and / or for deep cleaning of organic dirt, and methods for removing or reducing components of organic matter. Background Technology

[0006] Enzymes have been used in detergents for decades. A mixture of multiple enzymes is typically added to the detergent composition. Enzyme mixtures usually contain multiple enzymes, each targeting its specific substrate; for example, amylase is active against starch stains, and protease is active against protein stains. Textile surfaces and hard surfaces (such as the interior of dishes or washing machines) soiled with many different types of dirt (which can consist of proteins, grease, starch, etc.) undergo multiple washing cycles. One type of stain can be organic matter (such as biofilms, EPS, etc.). Organic matter can encompass various molecules (such as polysaccharides, extracellular DNA (eDNA), and proteins). Some organic matter forms an extracellular polymeric matrix, which can be sticky or adhesive; when present on textiles, it attracts dirt and can cause redeposition or reverse staining, resulting in a graying of the textile. Additionally, organic matter (such as biofilms) often causes odor problems because various odor molecules can adhere to and be slowly released from the complex extracellular matrix of polysaccharides, eDNA, and proteins, causing noticeable odor problems for consumers. There remains a need for cleaning compositions that effectively prevent, reduce, or remove organic contaminants, described herein as having a "deep cleaning" effect. This invention provides novel compositions that meet this need. Summary of the Invention

[0007] This invention relates to cleaning compositions comprising a DNase, a Glycohydro114 glycosyl hydrolase, and a cleaning component. The invention further relates to compositions (particularly cleaning compositions) comprising at least 0.001 ppm of DNase and at least 0.001 ppm of glycosyl hydrolase, and a cleaning component, wherein the cleaning component is selected from...

[0008] a. At least one surfactant, ranging from 0.1 wt% to 15 wt%;

[0009] b. at least one builder at a concentration of 0.5 wt% to 20 wt%; and

[0010] c. At least one bleaching agent component, ranging from 0.01 wt% to 10 wt%.

[0011] The present invention further relates to the use of a composition comprising a DNase, a Glycohydro114 glycosyl hydrolase, and a cleaning component for deep cleaning articles, wherein the articles are textiles or surfaces. The present invention further relates to a method of formulating a cleaning composition, the method comprising adding a DNase, a glycosyl hydrolase (preferably a Glycohydro114 glycosyl hydrolase), and at least one cleaning component. The present invention further relates to a kit intended for deep cleaning, wherein the kit comprises a solution containing an enzyme mixture comprising a DNase, a glycosyl hydrolase (preferably a Glycohydro114 glycosyl hydrolase), and optionally a protease. The present invention further relates to a method for deep cleaning articles, the method comprising the following steps:

[0012] a) Contact the article with a cleaning composition containing DNase, Glycohydro114 glycosyl hydrolase, and cleaning components; and

[0013] b) Optionally rinse the article, wherein the article is preferably a textile.

[0014] The present invention further relates to a method for deep cleaning articles, the method comprising the steps of: a) contacting the articles with a solution comprising an enzyme mixture containing DNase and glycosyl hydrolase and optionally a protease, and a cleaning component, wherein the cleaning component is selected from at least one surfactant selected from 0.1 wt% to 15 wt%; at least one detergent builder selected from 0.5 wt% to 20 wt%; and at least one bleach component selected from 0.01 wt% to 10 wt%; and b) optionally rinsing the articles, wherein the articles are preferably textiles. The present invention also relates to a kit intended for deep cleaning, wherein the kit comprises a solution containing an enzyme mixture containing DNase and Glycohydro-114 glycosyl hydrolase.

[0015] The present invention provides the following implementation scheme.

[0016] 1. A cleaning composition comprising a DNA enzyme, a Glycohydro114 glycosyl hydrolase, and a cleaning component.

[0017] 2. The cleaning composition according to embodiment 1, wherein the DNA enzyme is a microorganism, preferably obtained from bacteria or fungi.

[0018] 3. The cleaning composition according to embodiment 2, wherein the DNA enzyme is obtained from Bacillus, preferably from Bacillus foodae, Bacillus horikoshii, Bacillus licheniformis, Bacillus subtilis, Bacillus honek, Bacillus disease research institute, Bacillus hygroscopicus, Bacillus vinifera, Bacillus hanatsunabe, Bacillus indicus, Bacillus huanghaiensis, or Bacillus lucifera.

[0019] 4. The cleaning composition according to embodiment 3, wherein the DNAse comprises one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74).

[0020] 5. The cleaning composition according to any one of embodiments 2 to 4, wherein the DNA enzyme has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 13.

[0021] 6. The cleaning composition according to any one of embodiments 2 to 4, wherein the DNA enzyme has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 65.

[0022] 7. The cleaning composition according to any one of embodiments 2 to 4, wherein the DNA enzyme has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 66.

[0023] 8. The cleaning composition according to embodiment 2, wherein the DNase is fungal, preferably obtained from Aspergillus, and even more preferably from Aspergillus oryzae, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 67.

[0024] 9. The cleaning composition according to embodiment 2, wherein the DNase is fungal, preferably obtained from Trichoderma, and even more preferably from Trichoderma harzianum, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 68.

[0025] 10. The cleaning composition according to any one of the foregoing embodiments, wherein the glycosyl hydrolase is selected from the group consisting of amino acid sequences selected from the group consisting of:

[0026] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84.

[0027] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 85.

[0028] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 86.

[0029] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 87.

[0030] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 88.

[0031] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 89.

[0032] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0033] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 91.

[0034] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 92.

[0035] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0036] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0037] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 95.

[0038] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0039] n) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 97.

[0040] o) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0041] p) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0042] q) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0043] r) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0044] s) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0045] t) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0046] u) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0047] v) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0048] w) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 106.

[0049] x) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0050] y) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0051] z) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0052] aa) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0053] (bb) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0054] (cc) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0055] (dd) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0056] (ee) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0057] (ff) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0058] 11. The cleaning composition according to any one of the foregoing embodiments, wherein the amount of DNase in the composition is from 0.01 to 1000 ppm, and the amount of glycosyl hydrolase is from 0.01 to 1000 ppm.

[0059] 12. The cleaning composition according to any one of the foregoing embodiments, wherein the cleaning component is selected from surfactants, preferably anionic and / or nonionic surfactants, builder and bleaching components.

[0060] 13. The use of the cleaning composition according to any one of embodiments 1 to 12 for deep cleaning articles, wherein the articles are textiles or surfaces.

[0061] 14. A method for formulating a cleaning composition according to any one of embodiments 1 to 12, the method comprising adding a DNA enzyme, a glycosyl hydrolase preferably a Glycohydro-114 glycosyl hydrolase, and at least one cleaning component.

[0062] 15. A kit for deep cleaning, wherein the kit comprises a solution containing an enzyme mixture of DNase, Glycohydro114 glycosyl hydrolase and optionally protease.

[0063] 16. A method for deep cleaning articles, the method comprising the following steps:

[0064] a) Contacting the article with the cleaning composition according to any one of embodiments 1 to 12; and

[0065] b) Optionally rinse the article, wherein the article is preferably a textile. Detailed Implementation

[0066] Multiple enzymes are used in cleaning processes, each targeting a specific type of dirt (such as protein, starch, and grease stains). Enzymes are now standard ingredients in detergents used for washing clothes and dishes. The effectiveness of these commercial enzymes provides detergents that remove most types of dirt. However, due to the complex nature of such organic matter, organic matter contained in many biofilms (such as EPS (extracellular polymeric substances)) constitutes a challenging type of dirt. Commercially available cleaning compositions have not been effective in removing or reducing EPS and / or biofilm-related dirt, for example, on textiles. This document provides a cleaning composition comprising at least one DNase, at least one glycosyl hydrolase (e.g., Glycohydro114 glycosyl hydrolase), and a cleaning component. Biofilms can form when a group of microbial cells adhere to each other or to a surface (such as textiles, dishes, or hard surfaces) or another surface. These adherent cells are typically embedded in a self-generating matrix of extracellular polymeric substances (EPS), which constitute 50% to 90% of the total organic matter in the biofilm. EPS is primarily composed of polysaccharides (exopolysaccharides) and proteins, but also includes other macromolecules such as eDNA, lipids, and other organic matter. Organic matter (like biofilms) can be sticky or adhesive, and when present on textiles, it can cause redeposition or reverse staining of dirt, resulting in a graying of the textiles. Another drawback of organic matter (such as biofilms) is unpleasant odor, as many odor-related molecules are often associated with organic matter (such as biofilms). Furthermore, when dirty laundry items are washed together with less dirty laundry items, dirt present in the detergent solution tends to adhere to organic matter (such as biofilms or biofilm components), thus making the washed laundry items appear "dirtier" than before washing. This effect can also be called redeposition.

[0067] The compositions of the present invention are preferably cleaning compositions; the compositions comprise at least one DNase and at least one glycosyl hydrolase (e.g., Glycohydro114 glycosyl hydrolase). Examples of useful DNases and glycosyl hydrolases are referred to in the following sections: “peptides having DNase activity” and “peptides having glycosyl hydrolase activity”, respectively.

[0068] The compositions of the present invention comprise blends of DNases and glycosyl hydrolases (e.g., Glycohydro114 glycosyl hydrolases), which effectively reduce or remove organic components (e.g., DNA and polysaccharides).

[0069] enzymes

[0070] Polypeptides with DNase activity (DNase)

[0071] The term "DNase" refers to a polypeptide with DNase (deoxyribonuclease) activity that catalyzes the hydrolytic cleavage of phosphodiester bonds in the DNA backbone, thereby degrading DNA. Exonucleases cleave or cut residues at the ends of the DNA backbone, while endonucleases cleave or cut within the DNA backbone. DNases may cleave only double-stranded DNA or may cleave both double-stranded and single-stranded DNA. The terms "DNase" and the expression "polypeptide with DNase activity" are used interchangeably throughout this application. For the purposes of this invention, DNase activity is determined according to the procedure described in Assay I.

[0072] Preferably, the DNAse is selected from any of the enzymes EC3.1, preferably EC3.1.21, such as EC3.1.21.X (where X = 1, 2, 3, 4, 5, 6, 7, 8 or 9), or for example deoxyribonuclease I, deoxyribonuclease IV, type I site-specific deoxyribonuclease, type II site-specific deoxyribonuclease, type III site-specific deoxyribonuclease, CC-preferred endonuclease, deoxyribonuclease V, T(4) deoxyribonuclease II, T(4) deoxyribonuclease IV, or EC 3.1.22.Y (where Y = 1, 2, 4 or 5), such as deoxyribonuclease II, Aspergillus deoxyribonuclease K(1), crossover junction endonuclease, deoxyribonuclease X.

[0073] Preferably, the polypeptide with DNase activity is obtained from microorganisms, and the DNase is a microbial enzyme. The DNase is preferably of fungal or bacterial origin.

[0074] DNA enzymes can be obtained from Bacillus species, such as Bacillus licheniformis (B.). Bacillus subtilis ( ), Bacillus species-62451, Bacillus species-18318, Bacillus Horikoshi ( ), Bacillus species-62451, Bacillus species-16840, Bacillus species-62668, Bacillus species-13395, Bacillus honek ( ), Bacillus species-11238, food Bacillus ( ), Bacillus subtilis ( ), Bacillus species-62520, Bacillus species-16840, Bacillus species-62668, Bacillus algae ( ), Bacillus vinifera ( ), Bacillus thuringiensis ( ), Bacillus aureus ( ), Bacillus huanghaiense ( ), Bacillus lucifera ( ), Bacillus species SA2-6.

[0075] DNAase can also be obtained from any of the following: species of the genus *Echinochloa* ( ), , , , Multi-mastered spores ( ), species of the genus *Heterostylus* ( XZ1965, Peach brown rot fungus ( ), Curvularia crescentis ( ), Penicillium reticulatum ( ), quercetin penicillin ( ), Genus and species, Alternaria ( Alternaria species XZ2545, Trichoderma reesei, and Chaetomium thermophilum ( ), thermophilic chromospira ( ), , Cracked ring layer carbon shell fungus ( ), species of the genus *Ceratophyllum* ( XZ2007, species of the genus *Acer truncatum* XZ2414, *Acer truncatum dichromosum* ( ), species of the genus *Pterocarya* ( XZ2014, species of the genus Metarhizium ( HNA15-2, Clematis chinensis ( ), spiral spores ( ), Metarhizium anisopliae ( ), Diplosporium thermophilum ( ), , Environmental samples ( D, Environmental sample O, Environmental sample J, Clavicipitaceae species-70249, Westergren species ( AS85-2 , , , Lorva order ( ), genus *Cyclocarya* ( Trichoderma harzianum, Aspergillus oryzae, and Bacillus mucilaginosa ( ),or .

[0076] The DNase in the compositions used in this invention preferably belongs to the NUC1 group of DNases. The NUC1 group of DNases comprises polypeptides other than having DNase activity, and these polypeptides may contain one or more of the following motifs: [T / D / S][G / N]PQL (SEQ ID NO 69), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 70), or C[D / N]T[A / R] (SEQ ID NO: 71). One embodiment of the invention relates to a composition comprising a Glycohydro-114 glycosyl hydrolase and a polypeptide having DNase activity, wherein these polypeptides contain one or more of the following motifs: [T / D / S][G / N]PQL (SEQ ID NO 69), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 70) or C[D / N]T[A / R] (SEQ ID NO: 71).

[0077] These DNases preferably contain the NUC1_A domain [D / Q][I / V]DH (SEQ ID NO 72). In addition to containing any one of the domain motifs [T / D / S][G / N]PQL, [F / L / Y / I]A[N / R]D[L / I / P / V], or C[D / N]T[A / R], the DNase-active polypeptide used in the compositions of the present invention may contain the NUC1_A domain and may have a common motif [D / Q][I / V]DH (SEQ ID NO 72). In one embodiment, the present invention relates to a composition comprising a Glycohydro_114 glycosyl hydrolase and a polypeptide containing one or more motifs selected from the following: [T / D / S][G / N]PQL, [F / L / Y / I]A[N / R]D[L / I / P / V], C[D / N]T[A / R], and [D / Q][I / V]DH, wherein these polypeptides have DNase activity.

[0078] The DNases added to the compositions of the present invention preferably belong to the group of DNases contained in the GYS-clade, which is the group of DNases on the same branch of a phylogenetic tree having both structural and functional similarities. These NUC1 and / or NUC1_A DNases contain the conserved motif [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74) and share similar structural and functional properties. The GYS-clade DNases are preferably obtained from the genus Bacillus.

[0079] One embodiment of the present invention relates to a composition comprising a polypeptide comprising a Glycohydro-114 glycosyl hydrolase and a GYS clade having DNase activity, optionally wherein the polypeptide comprises one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) and ASXNRSKG (SEQ ID NO: 74), and wherein the polypeptide is selected from polypeptides of the group consisting of:

[0080] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1.

[0081] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 2.

[0082] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 3.

[0083] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 4.

[0084] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 5.

[0085] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 6.

[0086] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 7.

[0087] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 8.

[0088] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 9.

[0089] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 10.

[0090] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 11.

[0091] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 12.

[0092] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 13.

[0093] n) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 14.

[0094] o) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 15.

[0095] p) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 16.

[0096] q) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 17.

[0097] r) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 18.

[0098] s) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 19.

[0099] t) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 20.

[0100] u) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 21.

[0101] v) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 22.

[0102] w) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 23.

[0103] x) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 24, and

[0104] y) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 25.

[0105] Peptides possessing DNase activity and containing the GYS-clade motif have exhibited particularly good deep-cleaning properties; for example, these DNases are particularly effective at removing or reducing components of organic matter (such as biofilms) from items (such as textiles or hard surfaces). Furthermore, these DNases are particularly effective at removing or reducing odors from items (such as textiles or hard surfaces). In addition, GYS-clade DNases are particularly effective at preventing redeposition when washing items (such as textiles).

[0106] In one embodiment, the DNase to be added to the composition of the present invention preferably belongs to the group of DNases contained in the NAWK clade, which is NUC1 and NUC1_A DNases, and the DNase may further contain the conserved motif [V / I]PL[S / A]NAWK (SEQ ID NO: 75) or NPQL (SEQ ID NO: 76).

[0107] One embodiment of the present invention relates to a composition comprising a polypeptide comprising a Glycohydro114 glycosyl hydrolase and a NAWK-clade having DNase activity, optionally wherein the polypeptide comprises one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 75) or NPQL (SEQ ID NO: 76), and wherein the polypeptide is selected from polypeptides of the group consisting of:

[0108] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 26.

[0109] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 27.

[0110] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 28.

[0111] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 29.

[0112] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 30.

[0113] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 31.

[0114] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 32.

[0115] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 33.

[0116] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 34.

[0117] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 35.

[0118] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 36.

[0119] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 37, and

[0120] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 38.

[0121] Peptides possessing DNase activity and containing the NAWK-clade motif have exhibited particularly good deep-cleaning properties; for example, these DNases are particularly effective at removing or reducing components of organic matter (such as biofilms) from items (such as textiles or hard surfaces). Furthermore, these DNases are particularly effective at removing or reducing odors from items (such as textiles or hard surfaces). In addition, NAWK-clade DNases are particularly effective at preventing redeposition when washing items (such as textiles).

[0122] The DNase to be added to the composition of the present invention preferably belongs to the group of DNases contained in the KNAW clade, which is NUC1 and NUC1_A DNases, and the DNase may further contain the conserved motif P[Q / E]L[W / Y] (SEQ ID NO: 77) or [K / H / E]NAW (SEQ ID NO: 78).

[0123] One embodiment of the present invention relates to a composition comprising a polypeptide comprising a Glycohydro114 glycosyl hydrolase and a KNAW clade having DNase activity, optionally wherein the polypeptide comprises one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 77) or [K / H / E]NAW (SEQ ID NO: 78), and wherein the polypeptide is selected from polypeptides of the group consisting of:

[0124] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 39.

[0125] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 40.

[0126] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 41.

[0127] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 42.

[0128] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 43.

[0129] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 44.

[0130] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 45.

[0131] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 46.

[0132] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 47.

[0133] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 48.

[0134] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 49.

[0135] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 50, and

[0136] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 51.

[0137] Peptides possessing DNase activity and containing the KNAW-clade motif have exhibited particularly good deep-cleaning properties; for example, these DNases are particularly effective at removing or reducing components of organic matter (such as biofilms) from items (such as textiles or hard surfaces). Furthermore, these DNases are particularly effective at removing or reducing odors from items (such as textiles or hard surfaces). In addition, KNAW-clade DNases are particularly effective at preventing redeposition when washing items (such as textiles).

[0138] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-62451) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 1, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 1 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0139] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus Horikoshi) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 2, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 2 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0140] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-62520) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 3, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 3 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0141] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-62520) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 4, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 4 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0142] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus Horikoshi) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 5, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 5 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0143] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus Horikoshi) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 6, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 6 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0144] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-16840) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 7, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 7 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0145] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-16840) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 8, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 8 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0146] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-62668) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 9, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 9 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0147] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-13395) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 10, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 10 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0148] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus honekianis) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 11, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 11 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0149] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-11238) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 12, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 12 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0150] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus foodae) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 13, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 13 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0151] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species-18318) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 14, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 14 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0152] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus cereus) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 15, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 15 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0153] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus algae) having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 16, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 16 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0154] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from environmental sample J and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 17, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 17 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0155] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus viviparus) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 18, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 18 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0156] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus fusiformis) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 19, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 19 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0157] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Bacillus mucilaginosus* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 20, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 20 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0158] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus indicus) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 21, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 21 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0159] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus chrysogenum) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 22, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 22 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0160] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus lucifera) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 23, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 23 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0161] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus chrysogenum) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 24, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 24 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0162] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from the genus Bacillus (e.g., from Bacillus species SA2-6) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 25, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 25 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0163] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from a species of the genus *Echinochloa* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 26, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 26 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0164] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 27, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 27 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0165] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 28, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 28 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0166] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 29, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 29 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0167] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Cercospora multiflora* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 30, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 30 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0168] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from a species of the genus *Heterostigma* XZ1965 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 31, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 31 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0169] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Prunus syringae* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 32, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 32 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0170] In some embodiments, the present invention relates to compositions comprising a Glycohydro114 glycosyl hydrolase and a polypeptide obtained from Curvularia lunata and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 33, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 33 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0171] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Penicillium spp.* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 34, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 34 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0172] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Penicillium quercetin* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 35, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 35 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0173] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The species are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 36, and these compositions have DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 36 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0174] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from Alternaria species XZ2545 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 37, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 37 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0175] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from Alternaria and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 38, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 38 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0176] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Trichoderma reesei* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 39, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 39 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0177] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Chaetoceros thermophilus* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 40, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 40 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0178] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Chaetoceros thermophilus* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 41, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 41 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0179] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 42, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 42 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0180] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Cyclocarya spp.* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 43, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 43 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0181] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from a species of the genus *Cladosporium* XZ2007 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 44, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 44 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0182] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Acer dichromatosa* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 45, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 45 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0183] In some embodiments, the present invention relates to compositions comprising a Glycohydro114 glycosyl hydrolase and a polypeptide obtained from a species of the genus *Pleurotus* XZ2014 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 46, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 46 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0184] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from a species of *Metarhizium anisopliae* HNA15-2 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 47, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 47 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0185] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from a species of the genus *Cladosporium* XZ2414 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 48, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 48 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0186] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Bryum spp.* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 49, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 49 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0187] In some embodiments, the present invention relates to compositions comprising a Glycohydro114 glycosyl hydrolase and a polypeptide obtained from *Arthrium chinense* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 50, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 50 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0188] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Metarhizium anisopliae* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 51, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 51 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0189] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Diplostomum thermophilum* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 52, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 52 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0190] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 53, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 53 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0191] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 54, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 54 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0192] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from environmental sample D and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 55, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 55 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0193] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from an environmental sample O and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 56, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 56 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0194] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from Clavicipitaceae species-70249 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 57, and these compositions possess DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 57 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0195] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from a species of the genus *Westernia* AS85-2 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 58, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 58 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0196] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 59, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 59 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0197] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 60, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 60 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0198] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 61, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 61 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0199] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from the order Cladosporium and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 62, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 62 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0200] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide obtained from *Cyclocarya spp.* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 63, and these compositions having DNase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 63 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0201] In some embodiments, the present invention relates to a composition comprising a glycosylhydrolase and a polypeptide, the polypeptide being derived from... The peptides are available and have at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the peptide shown in SEQ ID NO: 64, and these compositions have DNase activity. In one aspect, these peptides differ from the mature peptide shown in SEQ ID NO: 64 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0202] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from the genus Bacillus (e.g., from Bacillus licheniformis) having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 65, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 65 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0203] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from the genus Bacillus (e.g., from Bacillus subtilis) having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 66, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 66 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0204] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from the genus *Aspergillus* (e.g., from *Aspergillus oryzae*) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 67, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 67 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0205] In some embodiments, the present invention relates to compositions comprising a glycosylhydrolase and a polypeptide, the polypeptide being available from the genus *Trichoderma* (e.g., *Trichoderma harzianum*) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 68, and the compositions having DNase activity. In one aspect, the polypeptides differ from the mature polypeptide shown in SEQ ID NO: 68 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0206] The above-mentioned DNA enzymes can be combined with any of the following glycosyl hydrolases to form blends added to the compositions according to the invention.

[0207] Polypeptides with glycosylation activity (glycosylated enzymes)

[0208] Glycosyl hydrolases (EC 3.2.1-) are a broad group of enzymes that hydrolyze glycosidic bonds between two or more sugars or between a sugar and a non-sugar moiety. Classification of glycosidic hydrolases within this family based on amino acid sequence similarity has been proposed. The polypeptides combined with a DNase and formulated into the detergent compositions of the present invention contain at least one glycosyl hydrolase domain and are defined as glycosyl hydrolases in the context of the present invention. Therefore, the polypeptides of the present invention hydrolyze glycosidic bonds, and the polypeptides of the present invention possess hydrolytic activity. The glycosyl hydrolase domain contained in the polypeptides of the present invention can be classified as Glyco_hydro_114 (Pfam domain id PF03537, Pfam version 31.0 Finn (2016). Nucleic AcidsResearch, Database Issue 44:D279-D285). The polypeptides of the present invention may further contain a polysaccharide deacetylase domain (CE4), and in preferred embodiments, the polypeptides of the present invention possess hydrolytic and / or deacetylase activity. The polypeptides of the present invention having hydrolytic and / or deacetylase activity include Glyco-hydro-114 glycosyl hydrolase. In the context of the present invention, Glyco-hydro-114 glycosyl hydrolase is a glycosyl hydrolase containing a glycosyl hydrolase domain (DUF297), referred herein as Glyco-hydro-114 (Pfam domain id PF03537, Pfam version 31.0 Finn (2016)). The polypeptides of the present invention are glycosyl hydrolases, preferably Glyco-hydro-114 glycosyl hydrolases. In a preferred embodiment, the polypeptides of the present invention are endopeptidase-α-1,4-polygalactosamineases. The polypeptides of the present invention have at least hydrolytic activity against glycosidic bonds and may also have deacetylase activity. In the context of the present invention, Glyco-hydro-114 glycosyl hydrolase is a PelA enzyme, which is active against polysaccharide bacterial membranes (present in many biofilms). Bacterial membrane (pellicle, pel) polysaccharides are, for example, those from *Pseudomonas aeruginosa* (…). The glycosyl hydrolase synthesized in the composition according to the invention is an important biofilm component that plays a key role in bacterial virulence and persistence. The biofilm is a cationic polymer composed of partially acetylated 1→4 glycosidic bonds of N-acetylgalactosamine and N-acetylglucosamine, which promotes cell-cell interactions within the biofilm matrix through electrostatic interactions with extracellular DNA (Jennings et al., PNAS, September 2015, Vol. 112, No. 36, 11353-11358; Marmont et al., J Biol Chem., November 24, 2017; 292(47):19411-19422.2017). The glycosyl hydrolase to be incorporated with the DNase in the composition according to the invention preferably belongs to the Glyco-hydro-114 pFam domain (PF03537) family. The glycosyl hydrolases to be incorporated into the compositions of the present invention preferably contain a domain referred to herein as the CE4_PelA_-like domain, these polypeptides being represented by the protein PelA, encoded by a gene in the pelA-G gene cluster, for producing and forming biofilms in *Pseudomonas aeruginosa*. PelA and most family members contain the domain DUF297 (PF03537) with an unknown function. The glycosyl hydrolases are preferably homologs of PelA.

[0209] Glycosyl hydrolases can protect against Pseudomonas Pf-5 ( Pseudomonas aeruginosa or Geobacter metallireducens are available. Preferably, the glycosyl hydrolase combined with the DNase of the present invention is any one of those shown in Table 1.

[0210] Table 1

[0211]

[0212] One embodiment of the invention relates to a composition comprising a DNase and a glycosyl hydrolase and a cleaning component, wherein the glycosyl hydrolase is a glycosyl hydrolase containing a Glyco_hydro_114 domain.

[0213] The glycosyl hydrolase combined with a DNase in the composition according to the invention comprises a GH domain, which can be classified as a Glyco-hydro-114 domain, and in a preferred embodiment, the polypeptide has hydrolytic (EC 3.2.1.) activity. ）The polypeptide containing the Glyco_hydro_114 domain is preferably a homolog of the PelA enzyme, which is a protein that degrades the extracellular polysaccharide Pel. In one embodiment, the glycosyl hydrolase is a glycosyl hydrolase containing the Glyco_hydro_114 domain, preferably from the genus Pseudomonas (such as Pseudomonas species-62208, *Pseudomonas seleniipraecipitans*, *Pseudomonas mikimotoi*). ), Pseudomonas ventricosa ( ), Pseudomonas aeruginosa ( ), Pseudomonas aeruginosa, Pseudomonas compostii ( Glycohydro-114 glycosyl hydrolase is preferably obtained from *Myxococcus* species (such as *Myxococcus macrosporum*). ), green myxococci ( ), Orange myxococcus ( ), petiole myxococcus ( Glycohydro114 glycosyl hydrolase can also preferably be obtained from any of the following organisms: Thermomyces hygrophila ( Burkholderia sp. species-63093, and Galicia galicum (a species of phytophaga) ), Coxs Nonomura ( ), *Rhutella lucosaminidum* ( ), Phenozanol's Burkholderia ( ), Streptomyces glaucus ( ), xylose-lysine-degrading Bacillus ( ), Bacillus subtilis (Ginseng soil swelling Bacillus) ), , boron-resistant lysine-tolerant bacillus (Lysinibacillus boronitolerans), microbulbifer hydrolyticus (Microbulbifer hydrolyticus), and Carnobacterium inhibens subsp.

[0214] In one embodiment, the present invention relates to a composition comprising a DNA enzyme, a glycosyl hydrolase, and a cleaning component, wherein the glycosyl hydrolase comprises a Glyco-hydro-114 glycosyl hydrolase domain.

[0215] The glycosylated enzyme preferably contains one or two motifs GX[FY][LYF]D (SEQ ID NO 82) or AYX[SET]XX[EAS] (SEQ ID NO: 83).

[0216] One embodiment of the present invention relates to a composition comprising a polypeptide having glycosyl hydrolase activity, optionally wherein the polypeptide optionally comprises one or both motifs GX[FY][LYF]D (SEQ ID NO 82) or AYX[SET]XX[EAS] (SEQ ID NO: 83), and wherein the polypeptide is selected from polypeptides of the group consisting of:

[0217] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84.

[0218] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 85.

[0219] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 86.

[0220] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 87.

[0221] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 88.

[0222] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 89.

[0223] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0224] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 91.

[0225] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 92.

[0226] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0227] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0228] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 95.

[0229] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0230] n) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 97.

[0231] o) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0232] p) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0233] q) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0234] r) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0235] s) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0236] t) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0237] u) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0238] v) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0239] w) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 106.

[0240] x) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0241] y) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0242] z) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0243] aa) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0244] (bb) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0245] (cc) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0246] (dd) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0247] (ee) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0248] (ff) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0249] In some preferred embodiments of the present invention, the DNA enzyme of the present invention is combined with a glycosyl hydrolase, wherein the glycosyl hydrolase is any one of the following:

[0250] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Pseudomonas* 62208 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 84, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 84 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0251] In some embodiments, the present invention relates to compositions comprising polypeptides available from environmental bacterial community A and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 85, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 85 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0252] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Thermomyces hygrophila* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 86, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 86 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0253] In some embodiments, the present invention relates to compositions comprising polypeptides available from environmental bacterial communities (LE) and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 87, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 87 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0254] In some embodiments, the present invention relates to compositions comprising polypeptides available from Burkholderia species-63093 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 88, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 88 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0255] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Myxococcus macrospora* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 89, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 89 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0256] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Gallicomonas galiculata* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 90, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 90 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0257] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Coccus nonomura* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 91, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 91 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0258] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Gastromycium ruthenicum* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 92, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 92 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0259] In some embodiments, the present invention relates to compositions comprising polypeptides available from environmental bacterial communities XE and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 93, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 93 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0260] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Burkholderia phenazine* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 94, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 94 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0261] In some embodiments, the present invention relates to compositions comprising polypeptides available from environmental bacterial communities and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 95, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 95 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0262] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Myxococcus viride* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 96, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 96 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0263] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Myxococcus orangeus* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 97, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 97 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0264] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Myxococcus macrospora* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 98, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 98 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0265] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Myxococcus petiolus* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 99, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 99 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0266] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Myxococcus macrospora* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 100, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 100 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0267] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Pseudomonas seleniipraecipitans* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 101, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 101 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0268] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Pseudomonas mifepristone* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 102, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 102 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0269] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Pseudomonas ventricosa* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 103, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 103 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0270] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Pseudomonas aeruginosa* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 104, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 104 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0271] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Pseudomonas aeruginosa* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 105, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 105 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0272] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Streptomyces griseus* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 106, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 106 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0273] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Bacillus xyloolysine* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 107, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 107 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0274] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Bacillus ginseng* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 108, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 108 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0275] In some embodiments, the present invention relates to compositions comprising polypeptides available from boron-resistant lysine-tolerant Bacillus and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 109, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 109 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0276] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Micrococcus hydrophila* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 110, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 110 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0277] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Carnivora spp.* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 111, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 111 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0278] In some embodiments, the present invention relates to compositions comprising polypeptides available from environmental bacterial subspecies and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 112, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 112 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0279] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Pseudomonas compostii* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 113, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 113 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0280] In some embodiments, the present invention relates to compositions comprising polypeptides available from *Burkholderia phenazine* and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 114, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 114 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0281] In some embodiments, the present invention relates to compositions comprising polypeptides available from Burkholderia species-63093 and having at least 60% (e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100%) sequence identity with the polypeptide shown in SEQ ID NO: 115, and these compositions possess glycosyl hydrolase activity. In one aspect, these polypeptides differ from the mature polypeptide shown in SEQ ID NO: 115 by up to 10 amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10).

[0282] A composition comprising:

[0283] This invention relates to cleaning compositions (e.g., detergent compositions) comprising the enzyme blends of the invention in combination with one or more additional cleaning composition components. The selection of these additional components is within the skill of a person skilled in the art and includes conventional ingredients, including exemplary non-limiting components described below. The enzyme blends of the invention comprise DNase and glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase). One embodiment of the invention relates to a cleaning composition comprising DNase, Glycohydro-114 glycosyl hydrolase, and cleaning components.

[0284] The DNase is preferably microbial, and preferably obtained from bacteria or fungi. One embodiment of the invention relates to a cleaning composition comprising a DNase, a Glycohydro114 glycosyl hydrolase, and a cleaning component, wherein the DNase is microbial, preferably bacterial or fungal.

[0285] In one embodiment, the DNase is obtained from bacteria. One embodiment of the invention relates to a cleaning composition comprising a DNase, a Glycohydro114 glycosyl hydrolase, and a cleaning component, wherein the DNase is obtained from Bacillus species (preferably Bacillus foodae, Bacillus Horikoshii, Bacillus licheniformis, Bacillus subtilis, Bacillus honek, Bacillus virginiae, Bacillus vinifera, Bacillus hanataniae, Bacillus indicus, Bacillus humilis, or Bacillus lucida).

[0286] Glycohydro-114 glycosyl hydrolases are preferably obtained from the genus *Pseudomonas* (such as *Pseudomonas* species-62208, *Seleniipraecipitans*, *Pseudomonas mikimotoi*, *Pseudomonas ventricosa*, *Pseudomonas scabra*, *Pseudomonas aeruginosa*, and *Pseudomonas compostii*). Glycohydro-114 glycosyl hydrolases are also preferably obtained from the genus *Myxococcus* (such as *Myxococcus macrosporus*, *Myxococcus virescens*, *Myxococcus fulvus*, and *Myxococcus stipitatus*). Glycohydro-114 glycosyl hydrolase can also preferably be obtained from any of the following organisms: *Thermus rehai*, *Burkholderia sp.*-63093, *Gallaecimonas pentacyclic aromaticativorans*, *Nonomuraeacoxensis*, *Glycomyces rutgersensis*, *Paraburkholderia phenazinium*, and *Streptomyces*. griseofuscus, xylooligosacillus xylanilyticus, ginsengisoli, boron-resistant lysine-tolerant bacillus, microbulbifer hydrolyticus, and Carnobacterium inhibens subsp. One embodiment of the present invention relates to a cleaning composition comprising a DNase, a Glycohydro114 glycosyl hydrolase, and a cleaning component, wherein the DNase is obtained from Bacillus species (preferably Bacillus foodae, Bacillus horikoshii, Bacillus licheniformis, Bacillus subtilis, Bacillus honek, Bacillus virginiae, Bacillus vinifera, Bacillus hanatsunabe, Bacillus indicus, Bacillus huanghaiensis, or Bacillus lucifera), and wherein the Glycohydro114 glycosyl hydrolase is obtained from Pseudomonas species (such as Pseudomonas species-62208, Pseudomonas seleniipraecipitans, Pseudomonas mibricus, Pseudomonas pleuropneumoniae, Pseudomonas aeruginosa, Pseudomonas compostii).Glycohydro-114 glycosyl hydrolase is preferably obtained from *Myxococcus* species (such as *Myxococcus macrosporus*, *Myxococcus virescens*, *Myxococcus fulvus*, and *Myxococcus stipitatus*). Glycohydro-114 glycosyl hydrolase can also preferably be obtained from any of the following organisms: *Thermophyton floccosum*, *Burkholderia* species-63093, *Gallicosoma galica*, *Cochliobolus nonomura*, *Gastrospermum ruthenicum*, *Phenyletonium parabukhoni*, *Streptomyces griseus*, *Bacillus xylose-lysine-degrading*, *Bacillus ginseng*, *Bacillus lysine-resistant*, *Micrococcus hydrolyticus*, and *Carnivora spp.*

[0287] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a Glycohydro114 glycosyl hydrolase, and a cleaning component, wherein the DNase is obtained from Bacillus species (preferably Bacillus foodae, Bacillus Horikoshii, Bacillus licheniformis, Bacillus subtilis, Bacillus honek, Bacillus virginiae, Bacillus vinifera, Bacillus hanatsunabe, Bacillus indicus, Bacillus huanghaiensis, or Bacillus lucifera), and wherein the Glycohydro114 glycosyl hydrolase is selected from the group consisting of:

[0288] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84.

[0289] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 85.

[0290] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 86.

[0291] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 87.

[0292] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 88.

[0293] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 89.

[0294] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0295] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 91.

[0296] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 92.

[0297] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0298] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0299] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 95.

[0300] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0301] n) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 97.

[0302] o) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0303] p) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0304] q) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0305] r) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0306] s) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0307] t) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0308] u) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0309] v) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0310] w) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 106.

[0311] x) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0312] y) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0313] z) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0314] aa) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0315] (bb) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0316] (cc) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0317] (dd) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0318] (ee) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0319] (ff) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0320] The DNase preferably belongs to the NUC1 group of DNases and contains one or more of the motifs [T / D / S][G / N]PQL (SEQ ID NO 69), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO 70), or C[D / N]T[A / R] (SEQ ID NO: 71). The DNase more preferably contains the NUC1_A domain [D / Q][I / V]DH (SEQ ID NO 72). Furthermore, the DNase may preferably contain any one of the domain motifs [T / D / S][G / N]PQL, [F / L / Y / I]A[N / R]D[L / I / P / V], or C[D / N]T[A / R]. The DNase added to the composition of the present invention preferably belongs to the group of DNases contained in the GYS-clade, which is the group of DNases on the same branch of a phylogenetic tree having both structural and functional similarities. These NUC1 and / or NUC1_A DNases contain conserved motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74) and share similar structural and functional properties. The DNases of the GYS clade are preferably obtained from Bacillus species. One embodiment of the invention relates to a cleaning composition comprising a DNase, a Glycohydro_114 glycosyl hydrolase, and a cleaning component, wherein the DNase contains one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74). One embodiment of the present invention relates to a cleaning composition comprising a DNase, a Glycohydro-114 glycosyl hydrolase, and a cleaning component, wherein the DNase preferably comprises one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74), and wherein the Glycohydro-114 glycosyl hydrolase is selected from the group consisting of:

[0321] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84.

[0322] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 85.

[0323] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 86.

[0324] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 87.

[0325] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 88.

[0326] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 89.

[0327] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0328] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 91.

[0329] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 92.

[0330] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0331] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0332] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 95.

[0333] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0334] n) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 97.

[0335] o) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0336] p) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0337] q) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0338] r) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0339] s) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0340] t) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0341] u) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0342] v) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0343] w) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 106.

[0344] x) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0345] y) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0346] z) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0347] aa) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0348] (bb) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0349] (cc) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0350] (dd) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0351] (ee) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0352] (ff) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0353] The glycosylated enzyme preferably contains one or two motifs GX[FY][LYF]D (SEQ ID NO 82) or AYX[SET]XX[EAS] (SEQ ID NO: 83).

[0354] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a Glycohydro114 glycosyl hydrolase, and a cleaning component, wherein the Glycohydro114 glycosyl hydrolase optionally comprises one or two motifs GX[FY][LYF]D (SEQ ID NO 82) or AYX[SET]XX[EAS] (SEQ ID NO: 83), and wherein the DNase optionally comprises one or two motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74), and wherein the DNase is selected from the group consisting of polypeptides comprising or composed of the following polypeptides:

[0355] a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1.

[0356] b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 2.

[0357] c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 3.

[0358] d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 4.

[0359] e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 5.

[0360] f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 6.

[0361] g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 7.

[0362] h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 8.

[0363] i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 9.

[0364] j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 10.

[0365] k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 11.

[0366] l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 12.

[0367] m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 13.

[0368] n) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 14.

[0369] o) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 15.

[0370] p) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 16.

[0371] q) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 17.

[0372] r) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 18.

[0373] s) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 19.

[0374] t) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 20.

[0375] u) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 21.

[0376] v) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 22.

[0377] w) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 23.

[0378] x) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 24, and

[0379] y) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 25.

[0380] The preferred DNase is a Bacillus DNase, such as Bacillus foodii, Bacillus subtilis, or Bacillus licheniformis.

[0381] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 13.

[0382] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 65.

[0383] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 66.

[0384] The DNase can also be fungal. One embodiment of the invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase is fungal, preferably obtained from Aspergillus and even more preferably from Aspergillus oryzae, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 67.

[0385] One embodiment relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase is fungal, preferably obtained from the Trichoderma genus and even more preferably from Trichoderma harzianum, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 68.

[0386] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the glycosyl hydrolase is selected from the group consisting of: Pseudomonas aeruginosa Pf-5 (GenBank: AAY92244), Pseudomonas aeruginosa (GenBank: AAG06452), and Sulfosporium thioretinoids (GenBank: ABB32191).

[0387] One embodiment of the present invention relates to a cleaning composition comprising a Bacillus DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the glycosyl hydrolase is selected from the group consisting of: Pseudomonas aeruginosa Pf-5 (GenBank: AAY92244), Pseudomonas aeruginosa (GenBank: AAG06452), and Sulfobacterium thioreduction (GenBank: ABB32191).

[0388] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the glycosyl hydrolase is selected from the group consisting of: Pseudomonas aeruginosa Pf-5 (GenBank: AAY92244), Pseudomonas aeruginosa (GenBank: AAG06452), and Sulfoviride-reducing bacteria (GenBank: ABB32191), and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 13.

[0389] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the glycosyl hydrolase is selected from the group consisting of: Pseudomonas aeruginosa Pf-5 (GenBank: AAY92244), Pseudomonas aeruginosa (GenBank: AAG06452), and Sulfosporium thioretinoids (GenBank: ABB32191), and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 65.

[0390] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the glycosyl hydrolase is selected from the group consisting of: Pseudomonas aeruginosa Pf-5 (GenBank: AAY92244), Pseudomonas aeruginosa (GenBank: AAG06452), and Sulfosporium thioretinoids (GenBank: ABB32191), and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 66.

[0391] The DNase can also be fungal. One embodiment of the invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the glycosyl hydrolase is selected from the group consisting of: *Pseudomonas aeruginosa* Pf-5 (GenBank: AAY92244), *Pseudomonas aeruginosa* (GenBank: AAG06452), and *Sulphurobacterium thioretinoides* (GenBank: ABB32191), wherein the DNase is fungal, preferably obtained from *Aspergillus* and even more preferably from *Aspergillus oryzae*, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 67.

[0392] One embodiment relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the glycosyl hydrolase is selected from the group consisting of: *Pseudomonas aeruginosa* Pf-5 (GenBank: AAY92244), *Pseudomonas aeruginosa* (GenBank: AAG06452), and *Sulphobicella sulfadiazine* (GenBank: ABB32191), wherein the DNase is fungal, preferably obtained from *Trichoderma* and even more preferably from *Trichoderma harzianum*, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO: 68.

[0393] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 13, and wherein the glycosyl hydrolase is selected from the group consisting of glycosyl hydrolases having the following amino acid sequence:

[0394] i) Sequence identity with the polypeptide shown in SEQ ID NO: 84 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0395] ii) Sequence identity with the polypeptide shown in SEQ ID NO: 85 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0396] iii) Sequence identity with the polypeptide shown in SEQ ID NO: 86 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0397] iv) Sequence identity with the polypeptide shown in SEQ ID NO: 87 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0398] v) The sequence identity with the polypeptide shown in SEQ ID NO: 88 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0399] vi) The sequence identity with the polypeptide shown in SEQ ID NO: 89 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0400] vii) at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0401] viii) The sequence identity with the polypeptide shown in SEQ ID NO: 91 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0402] (ix) The sequence identity with the polypeptide shown in SEQ ID NO: 92 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0403] x) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0404] (xi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0405] xii) The polypeptide shown in SEQ ID NO: 95 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0406] xiii) The polypeptide has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0407] xiv) The polypeptide shown in SEQ ID NO: 97 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0408] xv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0409] xvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0410] (xvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0411] xviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0412] (xix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0413] (xx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0414] (xxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0415] xxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0416] xxiii) The polypeptide shown in SEQ ID NO: 106 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0417] (xxiv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0418] (xxv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0419] (xxvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0420] (xxvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0421] (xxviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0422] (xxix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0423] (xxx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0424] (xxxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0425] xxxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0426] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 65, and wherein the glycosyl hydrolase is selected from the group consisting of glycosyl hydrolases having the following amino acid sequence:

[0427] i) Sequence identity with the polypeptide shown in SEQ ID NO: 84 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0428] ii) Sequence identity with the polypeptide shown in SEQ ID NO: 85 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0429] iii) Sequence identity with the polypeptide shown in SEQ ID NO: 86 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0430] iv) Sequence identity with the polypeptide shown in SEQ ID NO: 87 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0431] v) The sequence identity with the polypeptide shown in SEQ ID NO: 88 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0432] vi) The sequence identity with the polypeptide shown in SEQ ID NO: 89 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0433] vii) at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0434] viii) The sequence identity with the polypeptide shown in SEQ ID NO: 91 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0435] (ix) The sequence identity with the polypeptide shown in SEQ ID NO: 92 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0436] x) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0437] (xi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0438] xii) The polypeptide shown in SEQ ID NO: 95 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0439] xiii) The polypeptide has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0440] xiv) The polypeptide shown in SEQ ID NO: 97 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0441] xv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0442] xvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0443] (xvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0444] xviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0445] (xix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0446] (xx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0447] (xxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0448] xxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0449] xxiii) The polypeptide shown in SEQ ID NO: 106 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0450] (xxiv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0451] (xxv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0452] (xxvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0453] (xxvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0454] (xxviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0455] (xxix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0456] (xxx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0457] (xxxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0458] xxxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0459] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro-114 glycosyl hydrolase), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 66, and wherein the glycosyl hydrolase is selected from the group consisting of glycosyl hydrolases having the following amino acid sequence:

[0460] i) Sequence identity with the polypeptide shown in SEQ ID NO: 84 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0461] ii) Sequence identity with the polypeptide shown in SEQ ID NO: 85 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0462] iii) Sequence identity with the polypeptide shown in SEQ ID NO: 86 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0463] iv) Sequence identity with the polypeptide shown in SEQ ID NO: 87 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0464] v) The sequence identity with the polypeptide shown in SEQ ID NO: 88 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0465] vi) The sequence identity with the polypeptide shown in SEQ ID NO: 89 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0466] vii) at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0467] viii) The sequence identity with the polypeptide shown in SEQ ID NO: 91 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0468] (ix) The sequence identity with the polypeptide shown in SEQ ID NO: 92 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0469] x) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0470] (xi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0471] xii) The polypeptide shown in SEQ ID NO: 95 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0472] xiii) The polypeptide has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0473] xiv) The polypeptide shown in SEQ ID NO: 97 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0474] xv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0475] xvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0476] (xvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0477] xviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0478] (xix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0479] (xx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0480] (xxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0481] xxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0482] xxiii) The polypeptide shown in SEQ ID NO: 106 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0483] (xxiv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0484] (xxv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0485] (xxvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0486] (xxvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0487] (xxviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0488] (xxix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0489] (xxx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0490] (xxxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0491] xxxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0492] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro114), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 67, and wherein the glycosyl hydrolase is selected from the group consisting of glycosyl hydrolases having the following amino acid sequence:

[0493] i) Sequence identity with the polypeptide shown in SEQ ID NO: 84 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0494] ii) Sequence identity with the polypeptide shown in SEQ ID NO: 85 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0495] iii) Sequence identity with the polypeptide shown in SEQ ID NO: 86 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0496] iv) Sequence identity with the polypeptide shown in SEQ ID NO: 87 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0497] v) The sequence identity with the polypeptide shown in SEQ ID NO: 88 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0498] vi) The sequence identity with the polypeptide shown in SEQ ID NO: 89 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0499] vii) at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0500] viii) The sequence identity with the polypeptide shown in SEQ ID NO: 91 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0501] (ix) The sequence identity with the polypeptide shown in SEQ ID NO: 92 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0502] x) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0503] (xi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0504] xii) The polypeptide shown in SEQ ID NO: 95 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0505] xiii) The polypeptide has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0506] xiv) The polypeptide shown in SEQ ID NO: 97 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0507] xv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0508] xvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0509] (xvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0510] xviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0511] (xix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0512] (xx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0513] (xxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0514] xxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0515] xxiii) The polypeptide shown in SEQ ID NO: 106 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0516] (xxiv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0517] (xxv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0518] (xxvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0519] (xxvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0520] (xxviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0521] (xxix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0522] (xxx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0523] (xxxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0524] xxxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0525] One embodiment of the present invention relates to a cleaning composition comprising a DNase, a glycosyl hydrolase (preferably Glycohydro114), and a cleaning component, wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 68, and wherein the glycosyl hydrolase is selected from the group consisting of glycosyl hydrolases having the following amino acid sequence:

[0526] i) Sequence identity with the polypeptide shown in SEQ ID NO: 84 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0527] ii) Sequence identity with the polypeptide shown in SEQ ID NO: 85 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0528] iii) Sequence identity with the polypeptide shown in SEQ ID NO: 86 of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0529] iv) Sequence identity with the polypeptide shown in SEQ ID NO: 87 at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0530] v) The sequence identity with the polypeptide shown in SEQ ID NO: 88 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0531] vi) The sequence identity with the polypeptide shown in SEQ ID NO: 89 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0532] vii) at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 90.

[0533] viii) The sequence identity with the polypeptide shown in SEQ ID NO: 91 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0534] (ix) The sequence identity with the polypeptide shown in SEQ ID NO: 92 is at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.

[0535] x) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 93.

[0536] (xi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94.

[0537] xii) The polypeptide shown in SEQ ID NO: 95 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0538] xiii) The polypeptide has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96.

[0539] xiv) The polypeptide shown in SEQ ID NO: 97 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0540] xv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98.

[0541] xvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99.

[0542] (xvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 100.

[0543] xviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101.

[0544] (xix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.

[0545] (xx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103.

[0546] (xxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104.

[0547] xxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105.

[0548] xxiii) The polypeptide shown in SEQ ID NO: 106 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.

[0549] (xxiv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107.

[0550] (xxv) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108.

[0551] (xxvi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109.

[0552] (xxvii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110.

[0553] (xxviii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111.

[0554] (xxix) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112.

[0555] (xxx) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113.

[0556] (xxxi) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and

[0557] xxxii) has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115.

[0558] One embodiment of the present invention relates to a composition, for example, a composition comprising the following:

[0559] a) At least 0.001 ppm of at least one DNase, wherein the DNase is selected from the group consisting of:

[0560] i) A DNAse comprising one or more of the motifs [T / D / S][G / N]PQL (SEQ ID NO: 69), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 70), or C[D / N]T[A / R] (SEQ ID NO: 71);

[0561] ii) A DNAse containing the motif [D / Q][I / V]DH (SEQ ID NO 72);

[0562] iii) A DNase comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74);

[0563] iv) A DNase comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 75) or NPQL (SEQ ID NO: 76);

[0564] v) A DNAse containing one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 77) or [K / H / E]NAW (SEQ ID NO: 78);

[0565] vi) A DNAse, wherein the DNAse is selected from: a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 2; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 3; and a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1 ... The polypeptide shown in SEQ ID NO: 4 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 5; the polypeptide shown in SEQ ID NO: 6 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 7; and the polypeptide shown in SEQ ID NO: 8 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 5; and the polypeptide shown in SEQ ID NO: 6 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 7; and the polypeptide shown in SEQ ID NO: 8 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 8; and the polypeptide shown in SEQ ID NO: 9 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, The polypeptide shown in SEQ ID NO: 8 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 9, and the polypeptide shown in SEQ ID NO: 10 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 10.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 11; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 12; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 13; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1 ... The polypeptide shown in SEQ ID NO: 14 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16 ...5, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, The polypeptide shown in SEQ ID NO: 17 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 18, and the polypeptide shown in SEQ ID NO: 19 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 19, and the polypeptide shown in SEQ ID NO: 18, has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 19, and the polypeptide shown in SEQ ID NO: 19 ... The polypeptides shown in 20 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 21; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 22; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 23; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 21 ... The polypeptide shown in SEQ ID NO: 24 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 26 ...5, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 26, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, The polypeptide shown in SEQ ID NO: 27 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29 ..., and the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, The polypeptides shown in 30 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 31; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 32; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 33; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 31 ... The polypeptide shown in SEQ ID NO: 34 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 35, and the polypeptide shown in SEQ ID NO: 36 ... The polypeptide shown in SEQ ID NO: 37 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 38, and the polypeptide shown in SEQ ID NO: 39 ... The polypeptides shown in 40 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 41; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 42; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 43; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 41 ...2; a The polypeptide shown in SEQ ID NO: 44 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 46 ...5, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 46, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, The polypeptide shown in SEQ ID NO: 47 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 48, and the polypeptide shown in SEQ ID NO: 49 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 49, and the polypeptide shown in SEQ ID NO: 48 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 48, and the polypeptide shown in SEQ ID NO: 49 ... The polypeptides shown in the 50 list have sequence identity of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 51; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 52; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 53; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 51 ... The polypeptide shown in SEQ ID NO: 54 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 55, and the polypeptide shown in SEQ ID NO: 56 ... The polypeptide shown in SEQ ID NO: 57 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 58, and the polypeptide shown in SEQ ID NO: 59 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 59, and the polypeptide shown in SEQ ID NO: 58 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 58, and the polypeptide shown in SEQ ID NO: 59 ... The polypeptides shown in 60 have sequence identity of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 61; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 62; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 63; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 61 ... The polypeptide shown in SEQ ID NO: 64 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 65, and the polypeptide shown in SEQ ID NO: 6 ... The polypeptide shown in SEQ ID NO: 67 having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity, and the polypeptide shown in SEQ ID NO: 68 having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity, and,

[0566] b) At least 0.001 ppm of one or more glycosyl hydrolases, wherein the glycosyl hydrolases are selected from the group consisting of:

[0567] i) A glycosyl hydrolase containing one or two motifs GX[FY][LYF]D (SEQ ID NO: 82) or AYX[SET]XX[EAS] (SEQ ID NO: 83);

[0568] ii) A glycosyl hydrolase selected from polypeptides having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84, polypeptides having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 85, polypeptides having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 86, polypeptides having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84 ... The polypeptide shown in SEQ ID NO: 87 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 88, and the polypeptide shown in SEQ ID NO: 89 ... The polypeptide shown in SEQ ID NO: 90 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 91, and the polypeptide shown in SEQ ID NO: 92 ..., and the polypeptide shown in SEQ ID NO: 91, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 91, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, The polypeptides shown in 93 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 95; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94 ... The polypeptide shown in SEQ ID NO: 97 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98, and the polypeptide shown in SEQ ID NO: 99 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99, and the polypeptide shown in SEQ ID NO: 98 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98, and the polypeptide shown in SEQ ID NO: 9 ... The polypeptide shown in SEQ ID NO: 100 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101, and the polypeptide shown in SEQ ID NO: 102 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 103; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: The polypeptide shown in SEQ ID NO: 106 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 107, and the polypeptide shown in SEQ ID NO: 108 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108. The polypeptide shown in SEQ ID NO: 109 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 110, and the polypeptide shown in SEQ ID NO: 111 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1 ..., and the polypeptide shown in SEQ The polypeptide shown in 112 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 113; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114; and a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115;

[0569] iii) Glycosyl hydrolases selected from the group consisting of: *Pseudomonas aeruginosa* Pf-5 with GenBank number AAY92244, *Pseudomonas aeruginosa* with GenBank number AAG06452, and *Sulpho-reducing *Staphylococcus aureus* with GenBank number ABB32191; and

[0570] iv) Glycosyl hydrolases of the Glycohydro114 pFam domain (PF03537) family; and

[0571] c) At least one cleaning component, preferably selected from surfactants, detergents, bleaching agents, polymers and dispersants.

[0572] Optionally, the cleaning composition comprises at least 0.001 ppm of one or more proteases selected from the group consisting of the following:

[0573] i) A protease variant of the protease parent, wherein the protease is associated with SEQ ID NO 79 or SEQ ID NO Compared to the protease shown in 80, this protease variant contains one or more changes at one or more of the following positions: 3, 4, 9, 15, 24, 27, 42, 55, 59, 60, 66, 74, 85, 96, 97, 98, 99, 100, 101, 102, 104, 116, 118, 121, 126, 127, 128, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 189, 193, 198, 199, 200, 203, 206, 211, 212, 216, 218, 226, 229, 230, 239, 246, 255, 256, 268, and 269, where the position corresponds to SEQ ID NO. The location of the protease shown in SEQ ID NO 79, and wherein the protease variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98% but less than 100% sequence identity with SEQ ID NO 79 or SEQ ID NO 80;

[0574] ii) A protease variant of the protease parent, wherein the protease variant contains one or more mutations selected from the group consisting of: S3T, V4I, S9R, S9E, A15T, S24G, S24R, K27R, N42R, S55P, G59E, G59D, N60D, N60E, V66A, N74D, S85R, A96S, S97G, S97D, S97A, S97SD, S99E, S99D, S99G, S99M, S99N, S99R, S99H, S101A, V102I, V102Y, V102N, S104A, G116V, G116R, H118D, H118N, A12 0S, S126L, P127Q, S128A, S154D, A156E, G157D, G157P, S158E, Y161A, R164S, Q176E, N179E, S182E, Q185N, A188P, G189E, V193M, N198D, V199I, Y203W, S206G, L211Q, L211D, N212D, N212S, M216S, A226V, K229L, Q230H, Q239R, N246K, N255W, N255D, N255E, L256E, L256D, T268A, and R269H, where these positions correspond to SEQ The location of the protease shown in ID NO 79, wherein the protease variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98% but less than 100% sequence identity with SEQ ID NO 79 or SEQ ID NO 80;

[0575] iii) A protease comprising substitutions at one or more positions corresponding to positions 171, 173, 175, 179, or 180 of SEQ ID NO: 81 compared to the protease shown in SEQ ID NO: 81, wherein the protease variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% sequence identity with amino acid sequences 1 to 311 of SEQ ID NO: 81, but less than 100% sequence identity;

[0576] iv) A protease comprising the amino acid sequences shown in SEQ ID NO 79, 80, 81, 82, or a protease having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% but less than 100% sequence identity with the following polypeptides: a polypeptide comprising amino acids 1-269 of SEQ ID NO 79, a polypeptide comprising amino acids 1-311 of SEQ ID NO 81, or a polypeptide comprising amino acids 1-275 of SEQ ID NO 80;

[0577] v) One or more of the following protease variants selected from the group below:

[0578] SEQ ID NO 79 + T22R+S99G+S101A+V102I+A226V+Q239R、

[0579] SEQ ID NO 80 + S24G+S53G+S78N+S101N+G128A+Y217Q、

[0580] SEQ ID NO 80 + S24G+S53G+S78N+S101N+G128S+Y217Q、

[0581] SEQ ID NO 79 + S9E+ N42R+ N74D+ V199I+ Q200L+ Y203W+ S253D+ N255W+L256E、

[0582] SEQ ID NO 79 + S9E+N42R+N74D+H118V+Q176E+A188P+V199I+Q200L Y203W+S250D+S253D+N255W+L256E

[0583] SEQ ID NO 79 + S9E+N42R+N74D+Q176E+A188P+V199I+Q200L+Y203W S250D+S253D+N255W+L256E

[0584] SEQ ID NO 79 + S3V+N74D+H118V+Q176E+N179E+S182E+V199I+Q200L Y203W+S210V+S250D+S253D+N255W+L256E

[0585] SEQ ID NO 79 + T22A+N60D+S99G+S101A+V102I+N114L+G157D +S182D+T207A+A226V+Q239R+N242D+E265F、

[0586] SEQ ID NO 79 + S9E+N42R+ N74D+ H118V+Q176E+A188P+V199I+Q200L+ Y203W+S250D+ S253D+ N255W+ L256E、

[0587] SEQ ID NO 79 + S9E+N42R+ N74D+Q176E+A188P+V199I+Q200L+Y203W+ S250D+S253D+ N255W+ L256E、

[0588] SEQ ID NO 79 + S9E+ N42R+ N74D+ H118V+ Q176E+ A188P+V199I+ Q200L+Y203W+ S250D+ N255W+ L256E+ 269aH+ 269bH、

[0589] SEQ ID NO 79 + S3V+ N74D+ H118V+ Q176E+ N179E+ S182E+ V199I+ Q200L+Y203W+ S210V+ S250D+ N255W+ L256E、

[0590] SEQ ID NO 79 + S9E+ N74D+ G113W+ G157P+ Q176E+ V199I+ Q200L+ Y203W+S250D+ T254E+ N255W+ L256E、

[0591] SEQ ID NO 79 + S3V+ S9R+ N74D+ H118V+ Q176E+ N179E+ S182E+ V199I+Q200L+ Y203W + S212V+ S250D+ N255W+ L256E、

[0592] SEQ ID NO 79 +S99E、和

[0593] SEQ ID NO 80 +L217D。

[0594] The term "corresponds to" reflects the numbering system used, and various initiating proteases (parental proteases) can have different lengths. Therefore, a given initiating protease is compared with, for example, SEQ ID NO 79, and the position corresponding to, for example, pos 3 is determined.

[0595] SEQ ID NO XX + one or more mutations should be understood as variants of the protease parent containing a specific mutation (compared to the specific parent sequence), for example, SEQ ID NO 80 + L217D is a variant of the protease shown in SEQ ID NO 80 that, when compared with SEQ ID NO 80, contains the mutation L217D (with leucine replaced by aspartic acid at position 217 of SEQ ID NO 80).

[0596] The protease suitable for combination with the DNase in the compositions of the present invention is preferably any of the above-mentioned proteases, which are very useful in detergents and suitable for combination with DNase.

[0597] Glycosyl hydrolases and DNases can be included in the cleaning compositions of the present invention at the following levels: from 0.01 to 1000 ppm, from 1 ppm to 1000 ppm, from 10 ppm to 1000 ppm, from 50 ppm to 1000 ppm, from 100 ppm to 1000 ppm, from 150 ppm to 1000 ppm, from 200 ppm to 1000 ppm, from 250 ppm to 1000 ppm, from 250 ppm to 750 ppm, and from 250 ppm to 500 ppm. The above DNases can be combined with glycosyl hydrolases to form blends added to the washing solution according to the present invention. The concentration of DNase in the washing solution is typically in the range of 0.00001 ppm to 10 ppm, 0.00002 ppm to 10 ppm, 0.0001 ppm to 10 ppm, 0.0002 ppm to 10 ppm, 0.001 ppm to 10 ppm, 0.002 ppm to 10 ppm, 0.01 ppm to 10 ppm, 0.02 ppm to 10 ppm, 0.1 ppm to 10 ppm, 0.2 ppm to 10 ppm, and 0.5 ppm to 5 ppm. The concentration of Glycohydro114 glycosyl hydrolase in the washing solution is typically in the range of 0.00001 ppm to 10 ppm, 0.00002 ppm to 10 ppm, 0.0001 ppm to 10 ppm, 0.0002 ppm to 10 ppm, 0.001 ppm to 10 ppm, 0.002 ppm to 10 ppm, 0.01 ppm to 10 ppm, 0.02 ppm to 10 ppm, 0.1 ppm to 10 ppm, 0.2 ppm to 10 ppm, and 0.5 ppm to 5 ppm. The DNase can be combined with any of the glycosyl hydrolases mentioned above to form a blend added to the composition according to the invention.

[0598] One embodiment relates to a cleaning composition comprising a DNase, a Glycohydro-114 glycosyl hydrolase, and at least one cleaning component, wherein the amount of the DNase in the composition is from 0.01 to 1000 ppm, and the amount of the Glycohydro-114 glycosyl hydrolase is from 0.01 to 1000 ppm.

[0599] A method for formulating a cleaning composition comprising a DNase, a Glycohydro114 glycosyl hydrolase, and at least one cleaning component, the method comprising adding the DNase, the Glycohydro114 glycosyl hydrolase, and at least one cleaning component.

[0600] The selection of cleaning components may include (for textile care) the type of textile to be cleaned, the type and / or extent of soiling, the temperature at which cleaning is performed, and considerations for the formulation of the detergent product. Although the components mentioned below are classified under a general heading according to their specific functionality, this is not to be construed as limiting, as components may contain additional functionality as would be understood by a person skilled in the art.

[0601] A composition comprising:

[0602] a) At least 0.001 ppm of at least one DNase, wherein the DNase is selected from the group consisting of:

[0603] i) A DNAse comprising one or more of the motifs [T / D / S][G / N]PQL (SEQ ID NO: 69), [F / L / Y / I]A[N / R]D[L / I / P / V] (SEQ ID NO: 70), or C[D / N]T[A / R] (SEQ ID NO: 71);

[0604] ii) A DNAse containing the motif [D / Q][I / V]DH (SEQ ID NO 72);

[0605] iii) A DNase comprising one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74);

[0606] iv) A DNase comprising one or both of the motifs [V / I]PL[S / A]NAWK (SEQ ID NO: 75) or NPQL (SEQ ID NO: 76);

[0607] v) A DNAse containing one or both of the motifs P[Q / E]L[W / Y] (SEQ ID NO: 77) or [K / H / E]NAW (SEQ ID NO: 78);

[0608] vi) A DNAse, selected from the group consisting of: a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 2; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 3; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1 ... The polypeptide shown in SEQ ID NO: 4 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 5; the polypeptide shown in SEQ ID NO: 6 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 7; and the polypeptide shown in SEQ ID NO: 8 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 5; and the polypeptide shown in SEQ ID NO: 6 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 7; and the polypeptide shown in SEQ ID NO: 8 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 8; and the polypeptide shown in SEQ ID NO: 9 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, The polypeptide shown in SEQ ID NO: 8 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 9, and the polypeptide shown in SEQ ID NO: 10 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 10.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 11; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 12; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 13; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1 ... The polypeptide shown in SEQ ID NO: 14 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16 ..., and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, and the polypeptide shown in SEQ ID NO: 15, and the polypeptide shown in SEQ ID NO: 16, The polypeptide shown in SEQ ID NO: 17 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 18, and the polypeptide shown in SEQ ID NO: 19 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 19, and the polypeptide shown in SEQ ID NO: 18, has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 19, and the polypeptide shown in SEQ ID NO: 19 ... The polypeptides shown in 20 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 21; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 22; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 23; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 21 ... The polypeptide shown in SEQ ID NO: 24 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 26 ...5, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 26, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, and the polypeptide shown in SEQ ID NO: 25, The polypeptide shown in SEQ ID NO: 27 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29 ..., and the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 28, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, and the polypeptide shown in SEQ ID NO: 29, The polypeptides shown in 30 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 31; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 32; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 33; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 31 ...2; a The polypeptide shown in SEQ ID NO: 34 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 35, and the polypeptide shown in SEQ ID NO: 36 ... The polypeptide shown in SEQ ID NO: 37 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 38, and the polypeptide shown in SEQ ID NO: 39 ..., and the polypeptide shown in SEQ ID NO: 38, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 38, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, and the polypeptide shown in SEQ ID NO: 39, The polypeptides shown in 40 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 41; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 42; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 43; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 41 ...2; a The polypeptide shown in SEQ ID NO: 44 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 46 ...5, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 46, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, and the polypeptide shown in SEQ ID NO: 45, The polypeptide shown in SEQ ID NO: 47 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 48, and the polypeptide shown in SEQ ID NO: 49 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 49, and the polypeptide shown in SEQ ID NO: 48 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 48, and the polypeptide shown in SEQ ID NO: 49 ... The polypeptides shown in the 50 list have sequence identity of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 51; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 52; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 53; a ... The polypeptide shown in SEQ ID NO: 54 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 55, and the polypeptide shown in SEQ ID NO: 56 ... The polypeptide shown in SEQ ID NO: 57 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 58, and the polypeptide shown in SEQ ID NO: 59 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 59, and the polypeptide shown in SEQ ID NO: 58 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 58, and the polypeptide shown in SEQ ID NO: 59 ..., and the polypeptide shown in SEQ ID NO: 59, and the polypeptide shown The polypeptides shown in 60 have sequence identity of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100%.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 61; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 62; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 63; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 61 ... The polypeptide shown in SEQ ID NO: 64 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 65, and the polypeptide shown in SEQ ID NO: 6 ... The polypeptide shown in SEQ ID NO: 67 having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity, and the polypeptide shown in SEQ ID NO: 68 having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity, and,

[0609] b) At least 0.001 ppm of one or more glycosyl hydrolases, wherein the glycosyl hydrolases are selected from the group consisting of:

[0610] i) A glycosyl hydrolase containing one or two motifs GX[FY][LYF]D (SEQ ID NO: 82) or AYX[SET]XX[EAS] (SEQ ID NO: 83);

[0611] ii) A glycosyl hydrolase having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84, a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 85, a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 86, and a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 84 ... The polypeptide shown in SEQ ID NO: 87 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 88, and the polypeptide shown in SEQ ID NO: 89 ... The polypeptide shown in SEQ ID NO: 90 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 91, and the polypeptide shown in SEQ ID NO: 92 ..., and the polypeptide shown in SEQ ID NO: 91, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 91, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, and the polypeptide shown in SEQ ID NO: 92, The polypeptides shown in 93 have at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 95; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 96; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 94 ... The polypeptide shown in SEQ ID NO: 97 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98, and the polypeptide shown in SEQ ID NO: 99 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 99, and the polypeptide shown in SEQ ID NO: 98 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 98, and the polypeptide shown in SEQ ID NO: 9 ... The polypeptide shown in SEQ ID NO: 100 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 101, and the polypeptide shown in SEQ ID NO: 102 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102, and the polypeptide shown in SEQ ID NO: 101 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 102, and the polypeptide shown in SEQ ID NO: 102 ..., and the polypeptide shown in SEQ The polypeptide shown in 103 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 105; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 106; a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 104 ... The polypeptide shown in SEQ ID NO: 107 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108, and the polypeptide shown in SEQ ID NO: 109 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 109, and the polypeptide shown in SEQ ID NO: 108 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 108, and the polypeptide shown in SEQ ID NO: 109 ...8, and the polypeptide shown in SEQ The polypeptide shown in SEQ ID NO: 110 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 111, and the polypeptide shown in SEQ ID NO: 112 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 112, and the polypeptide shown in SEQ ID NO: 111 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 1 ... The polypeptide shown in 113 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity.A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and a polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 115;

[0612] iii) Glycosyl hydrolases selected from the group consisting of: *Pseudomonas aeruginosa* Pf-5 with GenBank number AAY92244, *Pseudomonas aeruginosa* with GenBank number AAG06452, and *Sulpho-reducing *Staphylococcus aureus* with GenBank number ABB32191; and

[0613] iv) Glycosyl hydrolases of the Glycohydro114 pFam domain (PF03537) family; and

[0614] c) At least one cleaning component, preferably selected from surfactants, detergents, bleaching agents, polymers and dispersants.

[0615] Optionally, the cleaning composition contains at least 0.001 ppm of one or more proteases, selected from...

[0616] i) A protease variant of the protease parent, wherein the protease is associated with SEQ ID NO 79 or SEQ ID NO Compared to the protease shown in 80, this protease variant contains one or more changes at one or more of the following positions: 3, 4, 9, 15, 24, 27, 42, 55, 59, 60, 66, 74, 85, 96, 97, 98, 99, 100, 101, 102, 104, 116, 118, 121, 126, 127, 128, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 189, 193, 198, 199, 200, 203, 206, 211, 212, 216, 218, 226, 229, 230, 239, 246, 255, 256, 268, and 269, where the position corresponds to SEQ ID NO. The location of the protease shown in SEQ ID NO 79, and wherein the protease variant has at least 80% sequence identity with SEQ ID NO 79, SEQ ID NO 80 or SEQ ID NO 81;

[0617] ii) A protease variant of the protease parent, wherein the protease variant contains one or more mutations selected from the group consisting of: S3T, V4I, S9R, S9E, A15T, S24G, S24R, K27R, N42R, S55P, G59E, G59D, N60D, N60E, V66A, N74D, N85S, N85R, G96S, G96A, S97G, S97D, S97A, S97SD, S99E, S99D, S99G, S99M, S99N, S99R, S99H, S101A, V102I, V102Y, V102N, S104A, G116V, G116R, H118D, H118 N, N120S, S126L, P127Q, S128A, S154D, A156E, G157D, G157P, S158E, Y161A, R164S, Q176E, N179E, S182E, Q185N, A188P, G189E, V193M, N198D, V199I, Y203W, S206G, L211Q, L211D, N212D, N212S, M216S, A226V, K229L, Q230H, Q239R, N246K, N255W, N255D, N255E, L256E, L256D, T268A, and R269H, where this position corresponds to SEQ. The location of the protease shown in ID NO 79, wherein the protease variant has at least 80% sequence identity with SEQ ID NO 79, SEQ ID NO 80 or SEQ ID NO 81;

[0618] iii) A protease comprising substitutions at one or more positions corresponding to positions 171, 173, 175, 179, or 180 of SEQ ID NO: 81 compared to the protease shown in SEQ ID NO: 81, wherein the protease variant has at least 75% but less than 100% sequence identity with amino acids 1 to 311 of SEQ ID NO: 81.

[0619] A protease comprising the amino acid sequence shown in SEQ ID NO 79, 80 or 81, or a protease having at least 80% sequence identity with the following: a polypeptide comprising amino acids 1-269 of SEQ ID NO 79, a polypeptide comprising amino acids 1-311 of SEQ ID NO 81, or a polypeptide comprising amino acids 1-275 of SEQ ID NO 80.

[0620] DNA enzymes can be combined with any of the Glycohydro-114 glycosyl hydrolases of the present invention to form blends added to compositions according to the present invention.

[0621] One embodiment relates to a cleaning composition comprising a DNase, a Glycohydro-114 glycosyl hydrolase, and at least one cleaning component, wherein the amount of the DNase in the composition is from 0.01 to 1000 ppm, and the amount of the Glycohydro-114 glycosyl hydrolase is from 0.01 to 1000 ppm.

[0622] In addition to Glycohydro114 glycosyl hydrolase and DNase, the cleaning composition further comprises one or more cleaning components. One embodiment relates to a cleaning composition comprising DNase, Glycohydro114 glycosyl hydrolase, and at least one cleaning component, wherein the cleaning component is selected from surfactants (preferably anionic and / or nonionic), builders, and bleaching components.

[0623] The selection of cleaning components may include (for textile care) the type of textile to be cleaned, the type and / or extent of soiling, the temperature at which cleaning is performed, and considerations for the formulation of the detergent product. Although the components mentioned below are classified under a general heading according to their specific functionality, this is not to be construed as limiting, as components may contain additional functionality as would be understood by a person skilled in the art.

[0624] surfactants

[0625] The detergent composition may contain one or more surfactants, which may be anionic and / or cationic and / or nonionic and / or semi-polar and / or zwitterionic, or mixtures thereof. In a specific embodiment, the detergent composition comprises a mixture of one or more nonionic surfactants and one or more anionic surfactants. The one or more surfactants are typically present at levels from about 0.1% to 60% by weight (e.g., about 1% to about 40%, or about 3% to about 20%, or about 3% to about 10%). The one or more surfactants are selected based on the desired cleaning application, and the one or more surfactants may include any conventional surfactants known in the art.

[0626] When included therein, the detergent will typically contain from about 1% to about 40% by weight of anionic surfactant, such as from about 5% to about 30%, including from about 5% to about 15%, or from about 15% to about 20%, or from about 20% to about 25% of anionic surfactant. Non-limiting examples of anionic surfactants include sulfates and sulfonates, specifically, linear alkylbenzene sulfonates (LAS), isomers of LAS, branched alkylbenzene sulfonates (BABS), phenyl alkyl sulfonates, α-olefin sulfonates (AOS), olefin sulfonates, chain olefin sulfonates, alkyl-2,3-dimethylbis(sulfate), hydroxyalkyl sulfonates and disulfonates, alkyl sulfates (AS) (such as sodium dodecyl sulfate (SDS)), fatty alcohol sulfates (FAS), primary alcohol sulfates (PAS), and alcohols. Ether sulfates (AES or AEOS or FES, also known as alcohol ethoxy sulfates or fatty alcohol ether sulfates), secondary alkyl sulfonates (SAS), paraffin sulfonates (PS), ester sulfonates, sulfonated fatty acid glycerides, α-sulfo fatty acid methyl esters (α-SFMe or SES) (including methyl ester sulfonates (MES)), alkyl succinic acids or alkenyl succinic acids, dodecenyl / tetradecenyl succinic acids (DTSA), fatty acid derivatives of amino acids, diesters and monoesters or fatty acid salts (soaps) of sulfosuccinic acids, and combinations thereof.

[0627] When included therein, the detergent will typically contain from about 1% to about 40% by weight of a cationic surfactant, for example from about 0.5% to about 30%, particularly from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12%, or from about 10% to about 12%. Non-limiting examples of cationic surfactants include alkyl dimethyl ethanol quaternary ammonium (ADMEAQ), hexadecyl trimethyl ammonium bromide (CTAB), dimethyl distearate ammonium chloride (DSDMAC), and alkyl benzyl dimethyl ammonium, alkyl quaternary ammonium compounds, alkoxylated quaternary ammonium (AQA) compounds, ester quaternary ammonium, and combinations thereof.

[0628] When included therein, the detergent will typically contain from about 0.2% to about 40% by weight of a nonionic surfactant, for example from about 0.5% to about 30%, particularly from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12%, or from about 10% to about 12%. Non-limiting examples of nonionic surfactants include alcohol ethoxylates (AE or AEO), alcohol propoxylates, propoxylated fatty alcohols (PFA), alkoxylated fatty acid alkyl esters (such as ethoxylated and / or propoxylated fatty acid alkyl esters), alkylphenol ethoxylates (APE), nonylphenol ethoxylates (NPE), alkyl polysaccharides (APG), alkoxylated amines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylated fatty acid monoethanolamides (EFAM), propoxylated fatty acid monoethanolamides (PFAM), polyhydroxyalkyl fatty acid amides, or N-acyl N-alkyl derivatives of glucosamine (glucosamide (GA) or fatty acid glucosamide (FAGA)), as well as products available under the trade names SPAN and TWEEN, and combinations thereof.

[0629] When included therein, the detergent will typically contain from about 0.01% to about 10% by weight of a semi-polar surfactant. Non-limiting examples of semi-polar surfactants include amine oxides (AOs), such as alkyl dimethylamine oxides, N-(cocoylalkyl)-N,N-dimethylamine oxides, and N-(butter-alkyl)-N,N-bis(2-hydroxyethyl)amine oxides, and combinations thereof.

[0630] When included therein, the detergent will typically contain from about 0.01% to about 10% by weight of a zwitterionic surfactant. Non-limiting examples of zwitterionic surfactants include betaines, such as alkyldimethyl betaine, sulfobetaine, and combinations thereof.

[0631] Builders and co-builders

[0632] The detergent composition may contain about 0-65% (e.g., about 5% to about 50%) of a detergent builder or co-builder, or a mixture thereof, by weight. In dishwashing detergents, the level of the builder is typically 40%-65%, particularly 50%-65%. The builder and / or co-builder may specifically be a chelating agent that forms a water-soluble complex having Ca and Mg. Any builder and / or co-builder known in the art for use in cleaning detergents may be used. Non-limiting examples of detergent builders include zeolites, diphosphates (pyrophosphates), triphosphates such as sodium triphosphate (STP or STPP), carbonates such as sodium carbonate, soluble silicates such as sodium silicate, layered silicates (e.g., SKS-6 from Hoechst), ethanolamines such as 2-aminoethanol (MEA), diethanolamine (DEA, also known as 2,2'-iminodiethanol), triethanolamine (TEA, also known as 2,2',2''-nitrotriethanol), and carboxymethyl inulin (CMI), and combinations thereof.

[0633] The detergent composition may also contain 0-50% by weight, such as about 5% to about 30%, of a detergent co-builder. The detergent composition may include a co-builder alone or in combination with a builder, such as a zeolite builder. Non-limiting examples of co-builders include homopolymers of polyacrylates or copolymers thereof, such as poly(acrylic acid) (PAA) or copolymers of (acrylic acid / maleic acid) (PAA / PMA). Other non-limiting examples include citrates, chelating agents (such as aminocarboxylates, aminopolycarboxylates, and phosphates), and alkylsuccinic acids or alkenylsuccinic acids. Other specific examples include 2,2',2''-nitroaminotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), iminodisuccinic acid (IDS), ethylenediamine-N,N'-disuccinic acid (EDDS), methylglycine diacetic acid (MGDA), glutamic acid-N,N-diacetic acid (GLDA), 1-hydroxyethane-1,1-diphosphonic acid (HEDP), ethylenediaminetetra(methylenephosphonic acid) (EDTMPA), diethylenetriaminepenta(methylenephosphonic acid) (DTMPA or DTPMPA), N-(2-hydroxyethyl)iminodiacetic acid (EDG), aspartic-N-monoacetic acid (ASMA), aspartic-N,N-diacetic acid (ASDA), aspartic-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA), N-(2-sulfomethyl)-aspartic acid (SMAS), N- (2-Sulfoethyl)-Aspartic acid (SEAS), N-(2-Sulfomethyl)-Glutamic acid (SMGL), N-(2-Sulfoethyl)-Glutamic acid (SEGL), N-Methyliminodiacetic acid (MIDA), α-Alanine-N,N-Diacetic acid (α-ALDA), Serine-N,N-Diacetic acid (SEDA), Isoserine-N,N-Diacetic acid (ISDA), Phenylalanine-N,N-Diacetic acid (PHDA), Anthranilic acid-N,N-Diacetic acid (ANDA), Sulfanic acid-N,N-Diacetic acid (SLDA), Taurine-N,N-Diacetic acid (TUDA), and sulfomethyl-N,N-Diacetic acid (SMDA), N-(2-hydroxyethyl)-ethylenediamine-N,N',N''-triacetic acid (HEDTA), diethanolglycine (DEG), diethylenetriaminepenta (DTPMP), aminotris(methylenephosphonic acid) (ATMP), and combinations thereof and their salts. Other exemplary builders and / or co-builders are described, for example, in WO 09 / 102854 and US 5977053.

[0634] bleaching system

[0635] The detergent may contain 0-30% by weight, such as about 1% to about 20%, of a bleaching system. Any bleaching system containing components known in the art for use in cleaning detergents can be used. Suitable bleaching system components include hydrogen peroxide sources; peracid sources; and bleaching catalysts or synergists.

[0636] Hydrogen peroxide source:

[0637] Suitable sources of hydrogen peroxide are inorganic peroxide salts, including alkali metal salts (such as sodium percarbonate and sodium perborate (usually monohydrate or tetrahydrate)) and hydrogen peroxide-urea (1 / 1).

[0638] Peracid source:

[0639] The peracid can be (a) directly incorporated as a pre-formed peracid, or (b) formed in situ in the washing solution from hydrogen peroxide and bleaching activator (perhydrolysis), or (c) formed in situ in the washing solution from hydrogen peroxide and catalase and a suitable substrate (e.g., ester) of the latter.

[0640] a) Suitable preformed peracids include, but are not limited to, peroxycarboxylic acids (such as peroxybenzoic acid) and their cyclically substituted derivatives, peroxy-α-naphthoic acid, peroxyphthalic acid, peroxylauric acid, peroxystearic acid, ε-phthaliminoperoxyhexanoic acid [phthaliminoperoxyhexanoic acid (PAP)], and o-carboxybenzoiminoperoxyhexanoic acid; aliphatic and aromatic diperoxydicarboxylic acids, such as diperoxydodecanoic acid, diperoxyazelaic acid, diperoxysediic acid, diperoxybasilicic acid, 2-decyl diperoxysuccinic acid, and diperoxyphthalic acid, -isophthalic acid and -terephthalic acid; perimino; peroxymonosulfuric acid; peroxydisulfuric acid; peroxyphosphoric acid; peroxysilicic acid; and mixtures of said compounds. It should be understood that, in some cases, the mentioned peracids may preferably be added as a suitable salt, such as an alkali metal salt (e.g., Oxone®) or an alkaline earth metal salt.

[0641] (b) Suitable bleaching activators include those belonging to the classes of esters, amides, imides, nitriles, or acid anhydrides, and, where applicable, their salts. Suitable examples are tetraacetyl ethylenediamine (TAED), sodium 4-[(3,5,5-trimethylhexanoyl)oxy]benzene-1-sulfonate (ISONOBS), sodium 4-(dodecanoyloxy)benzene-1-sulfonate (LOBS), sodium 4-(decanoyloxy)benzene-1-sulfonate, 4-(decanoyloxy)benzoic acid (DOBA), sodium 4-(nonanoyloxy)benzene-1-sulfonate (NOBS), and / or those disclosed in WO98 / 17767. Specific families of bleaching activators of interest are disclosed in EP624154, and in this family, triethyl acetylacetic acid (ATC) is particularly preferred. ATC, or short-chain triglycerides (like triacetin), have the advantage of being environmentally friendly. Furthermore, triethyl acetylglucosamine and triacetin exhibit good hydrolytic stability in the product during storage and are effective bleaching activators. Finally, ATC is versatile because the citrate released during the over-hydrolysis reaction can act as a detergent builder.

[0642] Bleaching catalysts and synergists

[0643] The bleaching system may also include a bleaching catalyst or synergist.

[0644] Some non-limiting examples of bleaching catalysts that can be used in the compositions of the present invention include manganese oxalate, manganese acetate, manganese collagen, cobalt-amine catalysts, and manganese triazacyclononane (MnTACN) catalysts; particularly preferred are complexes of manganese with 1,4,7-trimethyl-1,4,7-triazacyclononane (Me3-TACN) or 1,2,4,7-tetramethyl-1,4,7-triazacyclononane (Me4-TACN), specifically Me3-TACN, such as the binuclear manganese complex [(Me3-TACN)Mn(O)3Mn(Me3-TACN)](PF6)2, and [2,2',2''-nitrotris(ethane-1,2-diylazalkylyl-κN-methylyl)triphenol-κ3O]manganese(III). These bleaching catalysts can also be other metal compounds, such as iron or cobalt complexes.

[0645] In some embodiments, including those containing sources of excessive acid, an organic bleaching catalyst or bleaching synergist having one of the following formulas may be used:

[0646]

[0647] (iii) and mixtures thereof; wherein each R1 is independently a branched alkyl group containing 9 to 24 carbons or a straight-chain alkyl group containing 11 to 24 carbons, preferably each R1 is independently a branched alkyl group containing 9 to 18 carbons or a straight-chain alkyl group containing 11 to 18 carbons, more preferably each R1 is independently selected from the group consisting of: 2-propylheptyl, 2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, dodecyl, tetradecyl, hexadecyl, octadecyl, isononyl, isodecyl, isothidecyl, isothidecyl and isopentadecyl.

[0648] Other exemplary bleaching systems are described, for example, in WO 2007 / 087258, WO 2007 / 087244, WO 2007 / 087259, EP1867708 (Vitamin K), and WO 2007 / 087242. Suitable photobleaching agents may be, for example, sulfonated zinc phthalocyanine or aluminum phthalocyanine.

[0649] Metal care products

[0650] Metal care products can prevent or reduce rust, corrosion, or oxidation of metals, including aluminum, stainless steel, and non-ferrous metals such as silver and copper. Suitable examples include one or more of the following:

[0651] (a) Benzotriazoles, including benzotriazoles or bis-benzotriazoles and their substituted derivatives. Benzotriazole derivatives are those compounds in which the available substitution sites on the aromatic ring are partially or completely substituted. Suitable substituents include straight-chain or branched Ci-C20-alkyl groups (e.g., C1-C20-alkyl groups) and hydroxyl, thio, phenyl, or halogen (e.g., fluorine, chlorine, bromine, and iodine);

[0652] (b) Metal salts and complexes selected from the group consisting of: zinc, manganese, titanium, zirconium, hafnium, vanadium, cobalt, gallium, and cesium salts and / or complexes, wherein these metals are in one of oxidation states II, III, IV, V, or VI. In one aspect, suitable metal salts and / or metal complexes may be selected from the group consisting of: Mn(II) sulfates, Mn(II) citrates, Mn(II) stearates, Mn(II) acetylacetonates, K^TiF6 (e.g., K2TiF6), K^ZrF6 (e.g., K2ZrF6), CoSO4, Co(NOs)2, and Ce(NOs)3, zinc salts, such as zinc sulfate, zinc hydroxide, or zinc acetate;

[0653] (c) Silicates, including sodium silicate or potassium silicate, disodium silicate, sodium silicate, crystalline layered silicates and mixtures thereof.

[0654] WO 94 / 26860 and WO 94 / 26859 disclose other suitable organic and inorganic redox active substances for use as silver / copper corrosion inhibitors. Preferably, the compositions of the present invention comprise from 0.1% to 5% by weight of a metal care agent, preferably a zinc salt.

[0655] Water-soluble additives

[0656] Detergents may contain 0-10% by weight, for example 0-5% by weight, such as about 0.5% to about 5%, or about 3% to about 5% of water-soluble additives. Any water-soluble additive known in the art for use in detergents may be used. Non-limiting examples of water-soluble additives include sodium benzenesulfonate, sodium p-toluenesulfonate (STS), sodium xylenesulfonate (SXS), sodium cumenesulfonate (SCS), sodium cymene sulfonate, amine oxides, alcohols and polyethylene glycol ethers, sodium hydroxynaphthoate, sodium hydroxynaphthoate, sodium ethylhexyl sulfonate, sodium ethylhexyl sulfonate, and combinations thereof.

[0657] polymer

[0658] The detergent may contain 0-10% (e.g., 0.5%-5%, 2%-5%, 0.5%-2%, or 0.2%-1%) of a polymer by weight. Any polymer known in the art for use in detergents may be used. The polymer may function as a co-adjuvant as mentioned above, or may provide anti-redeposition, fiber protection, dirt release, dye transfer inhibition, oil stain removal, and / or antifoaming properties. Some polymers may have more than one of the properties mentioned above and / or more than one of the motifs mentioned below. Exemplary polymers include (carboxymethyl) cellulose (CMC), poly(vinyl alcohol) (PVA), poly(vinylpyrrolidone) (PVP), poly(ethylene glycol) or poly(ethylene oxide) (PEG), ethoxylated poly(ethyleneimine), carboxymethyl inulin (CMI), and polycarboxylates such as PAA, PAA / PMA, poly-aspartic acid, and lauryl methacrylate / acrylic acid copolymers, hydrophobically modified CMC (HM-CMC) and silicone, copolymers of terephthalic acid and oligomeric polyethylene glycol, copolymers of poly(ethylene terephthalate) and poly(ethylene oxyterephthalate) (PET-POET), PVP, poly(vinylimidazolium) (PVI), poly(vinylpyridine-N-oxide) (PVPO or PVPNO), and polyvinylpyrrolidone-vinylimidazolium (PVPVI). Suitable examples include PVP-K15, PVP-K30, ChromaBond S-400, ChromaBond S-403E, and ChromaBond S-100 from Ashl and Aqualon, and Sokalan® HP 165, Sokalan® HP 50 (dispersant), Sokalan® HP 53 (dispersant), Sokalan® HP 59 (dispersant), Sokalan® HP 56 (dye transfer inhibitor), and Sokalan® HP 66 K (dye transfer inhibitor) from BASF. Other exemplary polymers include sulfonated polycarboxylate, polyethylene oxide, and polypropylene oxide (PEO-PPO), as well as diquaternary ammonium ethoxysulfate. Other exemplary polymers are disclosed, for example, in WO 2006 / 130575. Salts of the polymers mentioned above are also considered. A particularly preferred polymer is the ethoxylated homopolymer Sokalan® HP 20 from BASF, which helps prevent redeposition of dirt in washing solutions.

[0659] Fabric colorant

[0660] The detergent compositions of the present invention may further include fabric colorants, such as dyes or pigments, which, when formulated in the detergent composition, can deposit on the fabric upon contact with a washing liquid containing the detergent composition, and thus alter the color of the fabric through absorption / reflection of visible light. Fluorescent whitening agents emit at least some visible light. In contrast, fabric colorants alter the color of a surface when they absorb at least a portion of the visible spectrum. Suitable fabric colorants include dyes and dye-clay conjugates, and may also include pigments. Suitable dyes include small molecule dyes and polymer dyes. Suitable small molecule dyes include those selected from the group consisting of dyes falling under the Colour Index (CI) classification: Direct Blue, Direct Red, Direct Violet, Acid Blue, Acid Red, Acid Violet, Basic Blue, Basic Violet, and Basic Red, or mixtures thereof, for example as described in WO 2005 / 03274, WO 2005 / 03275, WO 2005 / 03276 and EP1876226 (incorporated herein by reference). The detergent composition preferably comprises from about 0.00003 wt% to about 0.2 wt%, from about 0.00008 wt% to about 0.05 wt%, or even from about 0.0001 wt% to about 0.04 wt% of a fabric toner. The composition may contain from 0.0001 wt% to 0.2 wt% of a fabric toner, which may be particularly preferred when the composition is in the form of a unit-dosage pouch. Suitable toners are also disclosed, for example, in WO 2007 / 087257 and WO 2007 / 087243.

[0661] enzymes

[0662] The compositions of the present invention are preferably cleaning compositions and detergent compositions, and the compositions may contain one or more additional enzymes, such as one or more lipases, keratases, amylases, glycoses, cellulases, pectins, mannanases, arabinases, galactanases, xylanases, oxidases such as laccases, and / or peroxidases.

[0663] Generally, the properties of the selected one or more enzymes should be compatible with the selected detergent (i.e., optimal pH, compatibility with other enzymes and non-enzyme components, etc.), and the one or more enzymes should be present in an effective amount.

[0664] protease

[0665] The term "protease" is defined herein as an enzyme that hydrolyzes peptide bonds. It includes any enzyme belonging to the EC 3.4 enzyme group (including each of its 13 subclasses). EC number references are from Enzyme Nomenclature 1992 of NC-IUBMB, Academic Press, San Diego, California, included in supplements 1-5 of Eur. J. Biochem., 1223: 1-5 (1994); Eur. J. Biochem., 232: 1-6 (1995); Eur. J. Biochem., 237: 1-5 (1996); Eur. J. Biochem., 250: 1-6 (1997); and Eur. J. Biochem., 264: 610-650 (1999). The most widely used proteases in the detergent industry (such as laundry and dishwashing) are serine proteases. Serine proteases are a subgroup of proteases characterized by having a serine residue at their active site that forms a covalent adduct with the substrate. Serine proteases are characterized by having two amino acid residues at their active site besides serine: histidine and aspartic acid residues. According to Siezen et al., 1991, Protein Engineering 4: 719-737 and Siezen et al., 1997, Protein Science 6: 501-523, subtilisin refers to a subgroup of serine proteases. Subtilisin can be divided into six subfamilies: the subtilisin family, the thermophilic protease family, the proteinase K family, the lanoxanthiopeptidase family, the Kexin family, and the Pyrolysin family. The term "protease activity" refers to proteolytic activity (EC 3.4). The proteases that can be used in the cleaning compositions of this invention are primarily endopeptidases (EC 3.4.21). Several types of protease activity exist: the three main types are: trypsin-like, in which amide substrate cleavage occurs after Arg or Lys at P1; chymotrypsin-like, in which cleavage occurs after a hydrophobic amino acid at P1; and elastase-like, in which cleavage occurs after Ala at P1.

[0666] Suitable proteases for the compositions of the present invention include those of bacterial, fungal, plant, viral, or animal origin, such as plant or microbial origin. Microbial origin is preferred. This includes chemically modified mutants or protein-engineered mutants. It can be an alkaline protease, such as a serine protease or a metalloproteinase. Serine proteases can be, for example, from the S1 family (such as trypsin) or the S8 family (such as subtilisin). Metalloproteinases can be, for example, thermophilic bacterial proteases from the M4 family or other metalloproteinases, such as those from the M5, M7, or M8 families.

[0667] Examples of subtilisinases are those derived from the genus Bacillus, such as *Bacillus lentus*, *Bacillus alkalophilus*, *Bacillus subtilis*, *Bacillus amyloliquefaciens*, *Bacillus pumilus*, and *Bacillus giganteus* as described in US 7262042 and WO 09 / 021867; and *Lentus*, *Novo*, *Carlsberg*, *Bacillus licheniformis*, *BPN'*, *309*, *147*, and *168* as described in WO 89 / 06279, and *PD138* as described in (WO 93 / 18140). Other useful proteases may be those described in WO 92 / 175177, WO 01 / 016285, WO 02 / 026024, and WO 02 / 016547. Examples of trypsin-like proteases are trypsin (e.g., from pigs or cattle) and Fusarium proteases (described in WO 89 / 06270, WO 94 / 25583 and WO 05 / 040372), as well as chymotrypsin derived from Cellumonas (described in WO 05 / 052161 and WO 05 / 052146).

[0668] Further preferred proteases are alkaline proteases from Bacillus tarda DSM 5483 (as described in, for example, WO95 / 23221) and their variants (described in WO 92 / 21760, WO 95 / 23221, EP 1921147 and EP 1921148).

[0669] Examples of metalloproteinases are neutral metalloproteinases as described in WO 07 / 044993 (Genencor Int.), such as those derived from Bacillus amyloliquefaciens.

[0670] Examples of useful proteases are the variants described in the following: WO 92 / 19729, WO 96 / 034946, WO98 / 20115, WO 98 / 20116, WO 99 / 011768, WO 01 / 44452, WO 03 / 006602, WO 04 / 03186, WO 04 / 041979, WO 07 / 006305, WO 11 / 036263, WO 11 / 036264, particularly, contains substituted protease variants at one or more of the following positions: 3, 4, 9, 15, 24, 27, 42, 55, 59, 60, 66, 74, 85, 96, 97, 98, 99, 100, 101, 102, 104, 116, 118, 121, 126, 127, 128, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 189, 193, 198, 199, 200, 203, 206, 211, 212, 216, 218, 226, 229, 230, 239, 246, 255, 256, 268, and 269, where the position corresponds to SEQ ID NO. The location of the Bacillus retarded protease shown in 79. More preferred protease variants may comprise one or more mutations selected from the group consisting of: S3T, V4I, S9R, S9E, A15T, S24G, S24R, K27R, N42R, S55P, G59E, G59D, N60D, N60E, V66A, N74D, S85R, A96S, S97G, S97D, S97A, S97SD, S99E, S99D, S99G, S99M, S99N, S99R, S99H, S101A, V102I, V102Y, V102N, S104A, G116V, G116R, H118D, H118N, A1 20S, S126L, P127Q, S128A, S154D, A156E, G157D, G157P, S158E, Y161A, R164S, Q176E, N179E, S182E, Q185N, A188P, G189E, V193M, N198D, V199I, Y203W, S206G, L211Q, L211D, N212D, N212S, M216S, A226V, K229L, Q230H, Q239R, N246K, N255W, N255D, N255E, L256E, L256D, T268A, and R269H.These protease variants are preferably variants of Bacillus retarder protease shown in SEQ ID NO 1 of WO 2016 / 001449, or variants of Bacillus amyloliquefaciens protease (BPN') shown in SEQ ID NO 2 of WO 2016 / 001449. These protease variants preferably have at least 80% sequence identity with SEQ ID NO 1 or SEQ ID NO 2 of WO 2016 / 001449.

[0671] A substituted protease variant contained at one or more positions corresponding to positions 171, 173, 175, 179 or 180 of SEQ ID NO: 81, wherein the protease variant has at least 75% but less than 100% sequence identity with SEQ ID NO: 81.

[0672] Suitable commercially available proteases include those sold under the following trade names: Alcalase®, Duralase Tm Durazym Tm Relase®, Relase® Ultra, Savinase®, Savinase® Ultra, Primase®, Polarzyme®, Kannase®, Liquanase®, Liquanase® Ultra, Ovozyme®, Coronase®, Coronase® Ultra, Blaze®, Blaze Evity® 100T, Blaze Evity® 125T, Blaze Evity® 150T, Neutrase®, Everlase®, and Esperase® (Novozymes), those sold under the following trade names: Maxatase®, Maxacal®, Maxapem®, Purafect Ox®, Purafect OxP®, Puramax®, FN2®, FN3®, FN4®, Excellase®, ExcellenzP1000 TM Excellenz P1250 TM Eraser®, Preferenz P100 TM , Purafect Prime®, PreferenzP110 TM Effectenz P1000 TM Purafect® TM Effectenz P1050 TM PurafectOx® TM EffectenzP2000TM Purafast®, Properase®, Opticlean®, and Optimase® (Danisco / DuPont), Axapem TM (Gist-Brocases NV), BLAP (sequence shown in Figure 29 of US5352604) and its variants (Henkel AG) and KAP (Alkaliophilic Bacillus subtilis protease) from Kao Corporation.

[0673] Cellulase

[0674] Suitable cellulases include those of bacterial or fungal origin. This includes chemically modified mutants or protein-engineered mutants. Suitable cellulases include those from the genera *Bacillus*, *Pseudomonas*, *Pyrophyllus*, *Fusarium*, *Clostridium*, and *Apocytozoa*, such as the fungal cellulases produced by *Pyrophyllus*, *Thermophyllus*, and *Fusarium* as disclosed in US 4,435,307, US 5,648,263, US 5,691,178, US 5,776,757, and WO 89 / 09259.

[0675] Particularly suitable cellulases are alkaline or neutral cellulases that offer color-care benefits. Examples of such cellulases are those described in EP 0 495 257, EP 0 531 372, WO 96 / 11262, WO 96 / 29397, and WO 98 / 08940. Other examples are those cellulase variants described in WO 94 / 07998, EP 0 531 315, US 5,457,046, US 5,686,593, US 5,763,254, WO 95 / 24471, WO 98 / 12307, and WO99 / 001544.

[0676] Other cellulases are endoglucanases having the following sequence, which has at least 97% identity with the amino acid sequence at positions 1 to 773 of SEQ ID NO: 2 of WO 2002 / 099091, or family 44 xyloglucanases having the following sequence, which has at least 60% identity with positions 40-559 of SEQ ID NO: 2 of WO 2001 / 062903.

[0677] Commercially available cellulases include Celluzyme™ and Carezyme™ (Novozymes A / S), Carezyme Premium™ (Novozymes), Celluclean™ (Novozymes), CellucleanClassic™ (Novozymes), Cellusoft™ (Novozymes), Whitezyme™ (Novozymes), Clazinase™ and Puradax HA™ (Genencor International Inc.), and KAC-500(B)™ (Kao Corporation).

[0678] Mannanase

[0679] Suitable mannanases include those of bacterial or fungal origin. This includes chemically or genetically modified mutants. Mannanases can be basic mannanases of families 5 or 26. They can be wild-type from the genera *Bacillus* or *Pythium*, particularly *Bacillus mucosa*, *Bacillus licheniformis*, *Bacillus alkalophilus*, *Bacillus croceae*, or specific *Pythium* species. Suitable mannanases are described in WO 1999 / 064619. Commercially available mannanases are from Mannaway (Novozymes).

[0680] Peroxidase / oxidase

[0681] Suitable peroxidases / oxidases include those of plant, bacterial, or fungal origin. This includes chemically modified mutants or protein-engineered mutants. Examples of useful peroxidases include peroxidases from the genus *Coprinus* (e.g., *Coprinus spp.*) and their variants, such as those described in WO 93 / 24618, WO 95 / 10602, and WO 98 / 15257. Commercially available peroxidases include Guardzyme™ (Novozymes).

[0682] Lipase and keratinase:

[0683] Suitable lipases and keratins include those of bacterial or fungal origin. This includes chemically modified or protein-engineered mutant enzymes. Examples include lipases from the genus *Thermophilic*, such as those from *Thermophilic Hypotherium* (formerly named *Pythium spp.*) described in EP258068 and EP305216; keratins from the genus *Pythium*, such as *Pythium spp.* (WO96 / 13580); and lipases from strains of the genus *Pseudomonas* (some of which are now renamed *Burkholderia*), such as *Alcaligenes* or *Alcaligenes-like* (EP218272), *Pseudomonas cepacia* (EP331376), *Pseudomonas* strains SD705 (WO95 / 06720 and WO96 / 27002), and *Pseudomonas wisconsinii*. (sinensis) (WO96 / 12012); GDSL-type Streptomyces lipase (WO10 / 065455); cutinase from rice blast fungus (WO10 / 107560); cutinase from Pseudomonas mendoza (US5,389,536); lipase from Thermobifidafusca (WO11 / 084412); thermophilic Bacillus stearothermophilus lipase (WO11 / 084417); lipase from Bacillus subtilis (WO11 / 084599); and lipase from Streptomyces griseus (WO11 / 150157) and Streptomyces pristinaespiralis (WO12 / 137147).

[0684] Other examples are lipase variants, such as those described in EP407225, WO92 / 05249, WO94 / 01541, WO94 / 25578, WO95 / 14783, WO95 / 30744, WO95 / 35381, WO95 / 22615, WO96 / 00292, WO97 / 04079, WO97 / 07202, WO00 / 34450, WO00 / 60063, WO01 / 92502, WO07 / 87508, and WO09 / 109500.

[0685] Preferred commercially available lipase products include Lipolase TM Lipex™; Lipolex TM and Lipoclean TM (Novozymes), Lumafast (from Genencor), and Lipomax (from Gist-Brocades).

[0686] Other examples are lipases sometimes called acyltransferases or perhydrolases, such as an acyltransferase homologous to Candida antarctica lipase A (WO10 / 111143), an acyltransferase from Mycobacterium smegmatis (WO05 / 56782), a perhydrolase from the CE 7 family (WO09 / 67279), and variants of Mycobacterium smegmatis perhydrolases (particularly the S54V variant used in the commercial product Gentle Power Bleach from Huntsman Textile Effects Pte Ltd) (WO10 / 100028).

[0687] amylase:

[0688] Suitable amylases include α-amylases and / or glucosylamylases and may be of bacterial or fungal origin. This includes chemically modified mutants or protein-engineered mutants. Amylases include, for example, α-amylases obtained from specific strains of Bacillus, such as Bacillus licheniformis (described in more detail in GB 1,296,839).

[0689] Suitable amylases include the amylase having SEQ ID NO: 2 in WO 95 / 10603 or a variant thereof having 90% sequence identity with SEQ ID NO: 3. Preferred variants are described in WO 94 / 02597, WO 94 / 18314, WO 97 / 43424 and SEQ ID NO: 4 in WO 99 / 019467, such as variants having substitutions at one or more of the following positions: 15, 23, 105, 106, 124, 128, 133, 154, 156, 178, 179, 181, 188, 190, 197, 201, 202, 207, 208, 209, 211, 243, 264, 304, 305, 391, 408 and 444.

[0690] Suitable amylases include the amylase having SEQ ID NO: 6 in WO 02 / 010355 or a variant thereof having 90% sequence identity with SEQ ID NO: 6. Preferred variants of SEQ ID NO: 6 are those with deletions at positions 181 and 182 and substitutions at position 193.

[0691] Other suitable amylases are hybrid α-amylases comprising residues 1-33 of the α-amylase derived from *Bacillus amyloliquefaciens* as shown in SEQ ID NO: 6 of WO 2006 / 066594 and residues 36-483 of the α-amylase from *Bacillus licheniformis* as shown in SEQ ID NO: 4 of WO 2006 / 066594, or variants thereof having 90% sequence identity. Preferred variants of this hybrid α-amylase are those having substitutions, deletions, or insertions at one or more of the following positions: G48, T49, G107, H156, A181, N190, M197, I201, A209, and Q264. The most preferred variant of the hybrid α-amylase comprising residues 1-33 of the α-amylase derived from Bacillus amyloliquefaciens as shown in SEQ ID NO: 6 of WO 2006 / 066594 and residues 36-483 of the SEQ ID NO: 4 of WO 2006 / 066594 is those having the following substitutions:

[0692] M197T;

[0693] H156Y+A181T+N190F+A209V+Q264S; or

[0694] G48A+T49I+G107A+H156Y+A181T+N190F+I201F+A209V+Q264S.

[0695] Another suitable amylase is the amylase having SEQ ID NO: 6 in WO 99 / 019467 or a variant thereof having 90% sequence identity with SEQ ID NO: 6. Preferred variants of SEQ ID NO: 6 are those having substitutions, deletions, or insertions in one or more of the following positions: R181, G182, H183, G184, N195, I206, E212, E216, and K269. Particularly preferred amylases are those having deletions in positions R181 and G182, or positions H183 and G184.

[0696] Other amylases that can be used are those of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 2 or SEQ ID NO: 7 of WO 96 / 023873, or variants thereof having 90% sequence identity with SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 7. Preferred variants of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 7 are those with substitutions, deletions or insertions at one or more of the following positions: 140, 181, 182, 183, 184, 195, 206, 212, 243, 260, 269, 304 and 476, numbered using SEQ ID 2 of WO 96 / 023873. More preferred variants are those that have a deletion at two positions selected from 181, 182, 183, and 184 (e.g., 181 and 182, 182 and 183, or positions 183 and 184). The most preferred amylase variants of SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 7 are those that have a deletion at positions 183 and 184 and a substitution at one or more of positions 140, 195, 206, 243, 260, 304, and 476.

[0697] Other amylases that may be used are amylases having SEQ ID NO: 2 of WO 08 / 153815, SEQ ID NO: 10 of WO 01 / 66712, or variants thereof having 90% sequence identity with SEQ ID NO: 2 of WO 08 / 153815 or 90% sequence identity with SEQ ID NO: 10 of WO 01 / 66712. Preferred variants of SEQ ID NO: 10 of WO 01 / 66712 are those having substitutions, deletions, or insertions at one or more of the following positions: 176, 177, 178, 179, 190, 201, 207, 211, and 264.

[0698] Another suitable amylase is the amylase of SEQ ID NO: 2 with WO 09 / 061380 or a variant thereof having 90% sequence identity with SEQ ID NO: 2. Preferred variants of SEQ ID NO: 2 are those with C-terminal truncation and / or substitution, deletion, or insertion at one or more of the following positions: Q87, Q98, S125, N128, T131, T165, K178, R180, S181, T182, G183, M201, F202, N225, S243, N272, N282, Y305, R309, D319, Q320, Q359, K444, and G475. More preferred variants of SEQ ID NO: 2 are those having substitutions at one or more of the following positions: Q87E,R, Q98R, S125A, N128C, T131I, T165I, K178L, T182G, M201L, F202Y, N225E,R, N272E,R, S243Q,A,E,D, Y305R, R309A, Q320R, Q359E, K444E, and G475K, and / or those having deletions at positions R180 and / or S181 or T182 and / or G183. The most preferred amylase variants of SEQ ID NO: 2 are those having the following substitutions:

[0699] N128C+K178L+T182G+Y305R+G475K;

[0700] N128C+K178L+T182G+F202Y+Y305R+D319T+G475K;

[0701] S125A+N128C+K178L+T182G+Y305R+G475K; or

[0702] S125A+N128C+T131I+T165I+K178L+T182G+Y305R+G475K, wherein these variants are C-terminated and optionally further include a substitution at position 243 and / or a deletion at positions 180 and / or 181.

[0703] Another suitable amylase is the amylase of SEQ ID NO: 1 having WO 13184577 or a variant thereof having 90% sequence identity with SEQ ID NO: 1. Preferred variants of SEQ ID NO: 1 are those having substitutions, deletions, or insertions in one or more of the following positions: K176, R178, G179, T180, G181, E187, N192, M199, I203, S241, R458, T459, D460, G476, and G477. More preferred variants of SEQ ID NO: 1 are those having substitutions at one or more of the following positions: K176L, E187P, N192FYH, M199L, I203YF, S241QADN, R458N, T459S, D460T, G476K, and G477K, and / or those having deletions at positions R178 and / or S179 or T180 and / or G181. Most preferred amylase variants of SEQ ID NO: 1 are those having the following substitutions:

[0704] E187P+I203Y+G476K

[0705] E187P+I203Y+R458N+T459S+D460T+G476K

[0706] These variants optionally include a substitution at position 241 and / or a deletion at positions 178 and / or 179.

[0707] Another suitable amylase is the amylase of SEQ ID NO: 1 having WO10104675 or a variant thereof having 90% sequence identity with SEQ ID NO: 1. Preferred variants of SEQ ID NO: 1 are those having substitutions, deletions, or insertions at one or more of the following positions: N21, D97, V128, K177, R179, S180, I181, G182, M200, L204, E242, G477, and G478. More preferred variants of SEQ ID NO: 1 are those having substitutions at one or more of the following positions: N21D, D97N, V128I, K177L, M200L, L204YF, E242QA, G477K, and G478K, and / or those having deletions at positions R179 and / or S180 or I181 and / or G182. The most preferred amylase variant of SEQ ID NO: 1 is those with the following substitutions:

[0708] N21D+D97N+V128I

[0709] These variants optionally include a substitution at position 200 and / or a deletion at positions 180 and / or 181.

[0710] Other suitable amylases are α-amylases having SEQ ID NO: 12 in WO 01 / 66712 or variants having at least 90% sequence identity with SEQ ID NO: 12. Preferred amylase variants are those having substitutions, deletions, or insertions at one or more of the following positions in SEQ ID NO: 12 in WO 01 / 66712: R28, R118, N174; R181, G182, D183, G184, G186, W189, N195, M202, Y298, N299, K302, S303, N306, R310, N314; R320, H324, E345, Y396, R400, W439, R444, N445, K446, Q449, R458, N471, N484. Particularly preferred amylases include variants having deletions of D183 and G184 and having substitutions for R118K, N195F, R320K, and R458K, as well as variants having substitutions at one or more positions selected from the group consisting of M9, G149, G182, G186, M202, T257, Y295, N299, M323, E345, and A339, with the most preferred being variants having substitutions at all of these positions.

[0711] Other examples are amylase variants, such as those described in WO 2011 / 098531, WO 2013 / 001078 and WO 2013 / 001087.

[0712] Commercially available amylase is Duramyl TM Terminyl TM Fungayl TM Stainzyme TM StainzymePlus TM ...

Claims

1. A cleaning composition comprising a DNA enzyme, a Glycohydro114 glycosyl hydrolase, and a cleaning component.

2. The cleaning composition according to claim 1, wherein the DNA enzyme is a microorganism, preferably obtained from bacteria or fungi.

3. The cleaning composition according to claim 2, wherein the DNase is obtained from Bacillus, preferably from Bacillus foodae, Bacillus horikoshi, Bacillus licheniformis, Bacillus subtilis, Bacillus honek, Bacillus disease research institute, Bacillus algae, Bacillus vinifera, Bacillus hanatsunabe, Bacillus indicus, Bacillus huanghaiensis, or Bacillus lucifera.

4. The cleaning composition according to claim 3, wherein the DNAse comprises one or both of the motifs [D / M / L][S / T]GYSR[D / N] (SEQ ID NO: 73) or ASXNRSKG (SEQ ID NO: 74).

5. The cleaning composition according to any one of claims 2 to 4, wherein the DNAseed has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO 13.

6. The cleaning composition according to any one of claims 2 to 4, wherein the DNAseed has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO:

65.

7. The cleaning composition according to any one of claims 2 to 4, wherein the DNAseed has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO:

66.

8. The cleaning composition according to claim 2, wherein the DNase is fungal, preferably obtained from Aspergillus, and even more preferably from Aspergillus oryzae, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO:

67.

9. The cleaning composition according to claim 2, wherein the DNase is fungal, preferably obtained from Trichoderma, and even more preferably from Trichoderma harzianum, and wherein the DNase has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the amino acid sequence shown in SEQ ID NO:

68.

10. The cleaning composition according to any one of the preceding claims, wherein the glycosyl hydrolase is selected from the group consisting of amino acid sequences selected from the group consisting of: a) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

84. b) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

85. c) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

86. d) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

87. e) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

88. f) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

89. g) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

90. h) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

91. i) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

92. j) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

93. k) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

94. l) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

95. m) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

96. n) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

97. o) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

98. p) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

99. q) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

100. r) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

101. s) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

102. t) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

103. u) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

104. v) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

105. w) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

106. x) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

107. y) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

108. z) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

109. aa) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

110. (bb) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

111. (cc) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

112. (dd) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

113. (ee) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO: 114, and (ff) A polypeptide having at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or 100% sequence identity with the polypeptide shown in SEQ ID NO:

115.

11. The cleaning composition according to any one of the preceding claims, wherein the amount of DNase in the composition is from 0.01 to 1000 ppm, and the amount of glycosyl hydrolase is from 0.01 to 1000 ppm.

12. The cleaning composition according to any one of the preceding claims, wherein the cleaning component is selected from surfactants, preferably anionic and / or nonionic surfactants, detergent builders and bleaching components.

13. Use of the cleaning composition according to any one of claims 1 to 12 for deep cleaning articles, wherein the articles are textiles or surfaces.

14. A method for formulating a cleaning composition according to any one of claims 1 to 12, the method comprising adding a DNA enzyme, a glycosyl hydrolase preferably Glycohydro-114, and at least one cleaning component.

15. A kit for deep cleaning, wherein the kit comprises a solution containing an enzyme mixture of DNase, Glycohydro114 glycosyl hydrolase and optionally protease.

16. A method for deep cleaning articles, the method comprising the following steps: a) Contacting the article with the cleaning composition according to any one of claims 1 to 12; and b) Optionally rinse the article, wherein the article is preferably a textile.

Images