Illumination system and optical detection system suitable for nucleic acid detection devices

Abstract

This invention discloses an illumination system and an optical detection system suitable for nucleic acid detection devices. The illumination system includes a light source module, a field lens system, an excitation filter wheel, and an illumination prism module. The illumination prism module includes a beam splitter, multiple reflectors, at least two Dowell prisms, and at least two tilting reflectors. The light source module emits a beam to the field lens system for beam straightening and shaping, then filters it via the excitation filter wheel, and finally directs the beam into the beam splitter of the illumination prism module to separate it into at least two beams. The directions of the at least two beams are adjusted by the multiple reflectors, ensuring they pass through the at least two Dowell prisms and then through the at least two tilting reflectors, so that the at least two beams illuminate the sample's illumination area from different directions.

Description

Technical Field

[0001] This invention relates to an illumination system and an optical detection system, and more particularly to an illumination system and an optical detection system suitable for use in nucleic acid detection devices, having at least two illumination optical paths to improve the light uniformity of samples. Background Technology

[0002] The methods for obtaining large quantities of specific DNA fragments to meet diverse needs have flourished in recent years. Scientists have been striving to find efficient ways to achieve this goal, and polymerase chain reaction (PCR) is one of the most economical and rapid techniques, capable of obtaining billions of copies of specific DNA fragments in a short time. PCR technology has applications in various fields, such as selective DNA isolation for genetic identification, forensic analysis of ancient DNA in archaeology, medical applications in gene testing and tissue typing, rapid and accurate diagnosis of infectious diseases in hospitals and research institutions, environmental hazard testing for food safety, and genetic fingerprinting for investigating criminals. PCR technology only requires a small amount of DNA sample extracted from blood or tissue, and the addition of fluorescent dye to a nucleic acid solution allows for the detection of amplified DNA fragments through fluorescent molecules.

[0003] Dye and fluorescence detection technology is a widely used technique for the simultaneous detection and analysis of the presence of target nucleic acid molecules in a batch of biological samples. Under light excitation at a specific wavelength, the presence of a fluorescent signal emitted from a target nucleic acid molecule carrying a DNA-binding dye or a fluorescently bound probe indicates the presence of the target nucleic acid molecule. This technique is applied in modern PCR technology, known as the isothermal amplification method. Optical devices are indispensable tools in qPCR detection technology for detecting the fluorescence emitted by specific nucleic acid fragments. These optical devices must provide a light source to excite the fluorescent probe at a specific wavelength and simultaneously detect the fluorescent signal emitted from the probe.

[0004] However, in traditional qPCR detection techniques, one method is coaxial illumination, which primarily uses a dichroic mirror or beam splitter to refract light onto the sample. This method is mainly suitable for one or two detection channels. However, applying coaxial illumination to multi-channel detection significantly increases the design complexity of the dichroic mirror, thus limiting its application to only one or two detection channels and making multi-channel detection difficult. Furthermore, if a beam splitter is used, the light power is reduced by approximately 75% after passing through it, significantly affecting the illumination brightness. Therefore, for multi-channel detection, oblique illumination is usually used, where light illuminates the sample at a specific angle for multi-channel detection. However, oblique illumination has some undesirable optical characteristics, such as potential stray light, uneven illumination, and the possibility of shadows or afterimages on one side of the sample. However, in qPCR detection technology, low light power, uneven light, or shadows on the sample can all weaken the fluorescence signal of the sample, thus affecting the detection and interpretation results and leading to inaccurate results.

[0005] Therefore, in order to improve the defects of the optical detection system in the known qPCR detection technology, it is necessary to develop an illumination system and optical detection system suitable for nucleic acid detection devices that can effectively reduce shadows or afterimages caused by oblique illumination, improve light uniformity and light intensity, so as to make the detection results more accurate and reliable. Summary of the Invention

[0006] The purpose of this invention is to provide an illumination system suitable for nucleic acid detection devices, which has an illumination prism module to split a light beam from a light source module into at least two light beams, thereby providing at least two illumination light paths and illuminating the illumination area of ​​the sample from two different directions, so as to improve the uniformity and intensity of light on the sample, thereby making the detection results more accurate and reliable.

[0007] Another object of the present invention is to provide an illumination system suitable for nucleic acid detection devices, which includes a light source module, a field lens system, an excitation filter wheel, and an illumination prism module. The excitation filter wheel filters the white light emitted by the light source module and allows light beams of specific wavelengths to pass through, thereby limiting the illumination spectrum and reducing unnecessary light entering the illumination prism module, thereby reducing noise during detection.

[0008] Another objective of this invention is to provide an optical detection system suitable for nucleic acid detection devices, which includes an illumination system, a detection module, and a processor. The illumination system uses an illumination prism module to split a light beam from the light source module into at least two beams, thereby providing at least two illumination paths that illuminate the sample's illumination area from two different directions. This improves the uniformity and intensity of light on the sample, making the detection results more accurate and reliable. Furthermore, the compactly arranged illumination system reduces the overall volume of the optical detection system, making space utilization more flexible.

[0009] According to the concept of the present invention, an illumination system suitable for nucleic acid detection devices is provided, comprising: a light source module, a field lens system, an excitation filter wheel, and an illumination prism module. The field lens system is correspondingly disposed below the light source module. The illumination prism module comprises: a beam splitter, multiple reflectors, at least two Dowell prisms, and at least two tilting reflectors. The light source module emits a light beam to the field lens system for beam straightening and shaping, then filters it via the excitation filter wheel, and the beam enters the beam splitter of the illumination prism module to separate it into at least two beams. The directions of the at least two beams are adjusted by the multiple reflectors, so that they pass through the at least two Dowell prisms respectively, and then through the at least two tilting reflectors, so that the at least two beams illuminate the illumination area of ​​the sample in different directions.

[0010] According to the concept of the present invention, the light source module is a white light source module and includes a laser-driven white light source kit.

[0011] According to the concept of the present invention, the field lens system has a displaceable prism for beam collimation and shaping.

[0012] According to the concept of the present invention, the excitation filter wheel is disposed between the light source module and the illumination prism module to filter light and allow light of a specific wavelength to pass through.

[0013] According to the concept of the present invention, the illumination prism module further includes a first reflector, which is correspondingly disposed between the excitation filter wheel and the beam splitter to change the direction of the light beam and guide the light beam into the beam splitter.

[0014] According to the concept of the present invention, the plurality of reflectors are plurality of elliptical plane mirrors, used to guide at least two beams of light into at least two Dowell prisms.

[0015] According to the concept of the present invention, at least two of the Dove prisms are coated with a Vis 0° coating to finely adjust the orientation of at least two beams.

[0016] According to the concept of the present invention, at least two tilting mirrors are respectively disposed on a mirror mount, and each mirror mount is provided with a plurality of fine adjustment screws for precisely adjusting the tilt angle of the at least two tilting mirrors.

[0017] According to the concept of the present invention, the number of at least two Dowell prisms, at least two tilting mirrors, and at least two beams are all two. That is, the light source module emits the beam to the beam splitter to separate it into two beams, and the direction of the two beams is adjusted by multiple mirrors so that they pass through two Dowell prisms respectively, and then through two tilting mirrors, so that the two beams illuminate the illumination area of ​​the sample in different directions to achieve dual illumination.

[0018] According to the concept of the present invention, an optical detection system is provided, applicable to a nucleic acid detection device. The optical detection system includes an illumination system, a detection module, and a processor. The illumination system includes a light source module, a field lens system, an excitation filter wheel, and an illumination prism module. The field lens system is correspondingly disposed below the light source module. The illumination prism module includes a beam splitter, multiple reflectors, at least two Dowell prisms, and at least two tilting reflectors. The light source module emits a light beam to the field lens system for beam straightening and shaping, then filters it through the excitation filter wheel, and the beam enters the beam splitter of the illumination prism module to separate it into at least two beams. The directions of the at least two beams are adjusted by the multiple reflectors, so that they pass through the at least two Dowell prisms respectively, and then through the at least two tilting reflectors, so that the at least two beams illuminate the illumination area of ​​the sample in different directions. The detection module captures the image of the sample, and the processor analyzes it.

[0019] According to the concept of the present invention, the detection module includes a camera, and the camera includes one of a charge-coupled device, a complementary metal-oxide-semiconductor, and other digital imaging sensors. Attached Figure Description

[0020] Figure 1A This is a schematic diagram of the external structure of a lighting system according to a preferred embodiment of the present invention.

[0021] Figure 1B for Figure 1A The diagram shows a cross-sectional view of the lighting system.

[0022] Figure 2 for Figure 1A A schematic diagram of the internal optical path of the lighting system shown.

[0023] Figure 3 for Figure 2 A top view of the internal light path of the lighting system shown.

[0024] Figure 4 This is a schematic diagram of an optical detection system installed in a nucleic acid detection device according to a preferred embodiment of the present invention.

[0025] Figure 5 This is a cross-sectional structural diagram of the lighting system and detection module according to a preferred embodiment of the present invention.

[0026] The attached figures are labeled as follows:

[0027] 1: Lighting System

[0028] 10: Nucleic acid testing device

[0029] 100: Casing

[0030] 2: Light source module

[0031] 21: Laser-driven white light source kit

[0032] 3: Field Lens System

[0033] 30: Long sleeve

[0034] 31: Prism

[0035] 4: Excitation filter wheel

[0036] 5: Illumination Prism Module

[0037] 51: First reflecting mirror

[0038] 52: Beam splitter

[0039] 53, 531, 532, 533: Reflectors

[0040] 54, 541, 542: Dove Prism

[0041] 55, 551, 552: Tilted reflectors

[0042] 550: Mirror base

[0043] 553: Fine adjustment screw

[0044] 6: Sample

[0045] 6A: Lighting Area

[0046] 7: Detection Module

[0047] 8: Processor

[0048] L, L1, L2: Beam

[0049] L3: Fluorescent beam Detailed Implementation

[0050] Some typical embodiments embodying the features and advantages of the present invention will be described in detail in the following description. It should be understood that the present invention can be varied in different ways without departing from the scope of the invention, and the descriptions and illustrations herein are for illustrative purposes only and not for limiting the invention.

[0051] Please see Figure 1A, Figure 1B and Figure 2 . Figure 1A This is a schematic diagram of the external structure of a lighting system according to a preferred embodiment of the present invention. Figure 1B for Figure 1A The diagram shows a cross-sectional view of the lighting system. Figure 2 for Figure 1A A schematic diagram of the internal optical path of the lighting system shown. Figure 1A As shown, the lighting system 1 of the present invention is suitable for nucleic acid detection device 10 (e.g., Figure 4 As shown in the diagram. In this embodiment, the illumination system 1 includes: a light source module 2, a field lens system 3, an excitation filter wheel 4, and an illumination prism module 5. The field lens system 3 is correspondingly disposed below the light source module 2. Figure 1B As shown, the illumination prism module 5 includes: a beam splitter 52, multiple reflectors 53, at least two Dowell prisms 54, and at least two tilting reflectors 55. Figure 2 As shown, in this embodiment, the internal optical path of the lighting system 1 is mainly as follows: a light beam L is emitted from the light source module 2 to the field lens system 3 for beam straightening and shaping, then filtered by the excitation filter wheel 4, and the light beam L enters the beam splitter 52 of the lighting prism module 5 to be separated into at least two beams L1 and L2. The directions of the at least two beams L1 and L2 are adjusted by multiple reflectors 531, 532 and 533, so that they pass through at least two Dowell prisms 541 and 542 respectively, and then through at least two tilting reflectors 551 and 552, so that the at least two beams L1 and L2 illuminate the lighting area 6A of the sample 6 in different directions.

[0052] In this embodiment, the light source module 2 is a light source that emits broad-spectrum white light, such as a laser-driven white light source kit 21 (LS-WL1), but is not limited thereto. In other embodiments, the light source module 2 may also be other white light sources, such as LEDs or halogen lamps, and is not limited thereto. Figure 1BAs shown, the field lens system 3 is positioned below the light source module 2. In this embodiment, the field lens system 3 is a long sleeve 30 containing a movable prism 31. This means that the prism 31 can be adjusted vertically within the long sleeve 30 according to actual needs, thereby straightening and shaping the light beam L entering the field lens system 3, and then guiding the light beam L vertically downward into the excitation filter wheel 4. In this embodiment of the field lens system 3, the excitation filter wheel 4 is positioned between the field lens system 3 and the illumination prism module 5 to filter light and allow light of a specific wavelength to pass through. For example, the excitation filter wheel 4 in this embodiment can be, but is not limited to, an electric filter wheel (Thorlabs FW102C), which can be rotated to replace different filters, thereby filtering light according to different filters. That is, it can only allow light beams of a specific wavelength L to pass through, thus limiting the illumination spectrum and reducing unnecessary light entering the illumination prism module 5, thereby reducing noise during detection. In other words, this embodiment can rotate to the corresponding excitation filter wheel 4 according to the different fluorescence required to be excited by different samples 6. When the light beam L passes through the set filter, it is at the required specific wavelength (e.g., red light, yellow light, blue light). The wavelength-adjusted light beam L is then guided into the illumination prism module 5, thereby customizing the wavelength of the required light beam L and thus exciting the required specific fluorescence. Of course, in other embodiments, the excitation filter wheel 4 can also use other types of filter wheels, or manually interchangeable filters, etc., and is not limited thereto. However, in this embodiment, the use of an electric filter wheel allows for quick filter replacement and effectively reduces the volume of the excitation filter wheel 4, making the overall optical detection system smaller and more compact, without occupying extra space.

[0053] Please refer to further information. Figure 1B , Figure 2 , Figure 3 . Figure 3 for Figure 2 A top view of the internal light path of the lighting system shown. (See attached image.) Figure 1BAs shown, after the light source module 2 emits a beam L, it is straightened and shaped by the field lens system 3, filtered and its wavelength adjusted by the excitation filter wheel 4, and then vertically guided downward into the illumination prism module 5. In some embodiments, after the vertical beam L enters the illumination prism module 5, it can be directly guided into the beam splitter 52 for beam splitting. In other embodiments, the illumination prism module 5 may have a first reflector 51, but is not limited thereto. Taking this embodiment as an example, the first reflector 51 of the illumination prism module 5 is correspondingly disposed below the excitation filter wheel 4 to convert the vertical beam L into a horizontal path direction and guide it into the beam splitter 52. In this embodiment, the first reflector 51 may be, but is not limited to, a lens coated with reinforced aluminum film and with a flatness of 4-6λ, and is not limited thereto. This angled first reflector 51 is mainly used to change the path direction of the beam L and can reduce the overall size of the system. Of course, as mentioned earlier, the configuration can be adjusted according to different optical path planning in different system configurations, and it is not always necessary to set up a first reflecting mirror 51. Please refer to Figure 2 In this embodiment, the beam splitter 52 may be, but is not limited to, using 25mm Etmont optical technology. The non-polarizing coaxial cubic beam splitter is used, but is not limited to this, and its reflection / transmission ratio (R / T) is 50 / 50. In this embodiment, it is mainly used for dual illumination, with beam splitter 52 used to split the beam L into two beams L1 and L2 for dual illumination, providing better results. However, in other embodiments, such as providing multi-illumination, such as three- or four-directional illumination, beam splitter 52 will naturally split the single beam L into three or four beams, or multiple beam splitters 52 can be used for beam splitting, which can be varied according to different needs. In this embodiment, after the beam is split into L1 and L2, it enters different reflectors 531, 532, and 533 respectively. Taking this embodiment as an example, beam L1 enters the corresponding Dowell prism 541 after being reflected by reflector 531; while the other beam L2 enters the corresponding Dowell prism 542 after being reflected by two reflectors 532 and 533. In some embodiments, reflectors 531, 532, and 533 may be, but are not limited to, elliptical plane mirrors. For example, this embodiment employs Ertmont Optics. The λ / 8 precision elliptical plane mirror, whose size can be, but is not limited to, 31.75 mm, is coated with protective silver, but is not limited thereto. The main function of reflectors 531, 532, and 533 is to adjust the paths of the two beams L1 and L2 after beam splitting and guide them back into the Dowell prism 54. Therefore, the number, position, shape, or coating of the multiple reflectors 531, 532, and 533 can be varied according to the actual optical path design requirements and is not limited thereto.

[0054] Please continue reading. Figure 2 and Figure 3In this embodiment, the main illumination is dual illumination, using two Dowell prisms 54, namely two Dowell prisms 541 and 542. As shown in the figure, the two beams L1 and L2 after beam splitting are guided into Dowell prisms 541 and 542 respectively after adjustment by multiple reflectors 531, 532, and 533. Taking this embodiment as an example, the Dowell prisms 541 and 542 are trapezoidal and coated with a Vis 0° coating, such as those from Etmont Optics (Vis 0°). The Dowell prism 54 is used to rotate and adjust beams L1 and L2, thereby ensuring that the two beams L1 and L2 illuminate the sample 6 in the same direction, i.e., to fine-tune the orientation of the two beams L1 and L2. Of course, in other embodiments, Dowell prisms with other coatings can also be used, and this is not a limitation. In this embodiment, the main function of the Dowell prism 54 is to fine-tune the direction of different light rays, so that they converge and point in the same direction, so that when they illuminate the sample 6, the uniform illumination area can be maximized. After the two beams L1 and L2 are adjusted by the Dowell prisms 541 and 542 respectively, they are then adjusted by the optical path of the two tilting mirrors 551 and 552, so that the beams L1 and L2 are concentrated in the illumination area 6A of the sample 6 in different directions and angles. In this embodiment, there are two tilting mirrors 551 and 552, which mainly correspond to the number of Dowell prisms 54. And as Figure 3 As can be seen, after adjustment by the two tilting mirrors 551 and 552, the light beams L1 and L2 illuminate and converge on the illumination area 6A of the sample 6 in two opposite directions, thereby providing light from the two opposite sides of the illumination area 6A and effectively reducing the shadows on the sample 6. In some embodiments, the tilting mirror 55 is disposed on the mirror base 550 (e.g., Figure 1A and Figure 1B As shown), but not limited to this, and each lens mount 550 is provided with multiple fine adjustment screws 553 (such as... Figure 1A and Figure 1B As shown), the tilt angle of the tilting mirror 55 is precisely adjusted. In this embodiment, for example, a 1.0-inch small mirror mount 550 from Atmont Optics is used, and the fine adjustment screws 553 on the mount 550 are used to precisely adjust the tilt of the mounting surface in at least two directions, thereby adjusting the tilt angle of the two tilting mirrors 55. This allows the light beams L1 and L2 to be precisely aligned with the illumination area 6A on the sample 6 after reflection by the two tilting mirrors 55. Of course, in other embodiments, other types of mirrors with or without adjustable mounts can be used, and the implementation can vary depending on the actual situation, and is not limited thereto.

[0055] Please also refer to Figure 3 and Figure 4 . Figure 4This is a schematic diagram illustrating the optical detection system of a preferred embodiment of the present invention, installed within a nucleic acid detection device. Figure 4 As shown, the optical detection system of this embodiment is applicable to a nucleic acid detection device 10, and the optical detection system includes an illumination system 1, a detection module 7, and a processor 8. The illumination system 1 includes a light source module 2, a field lens system 3, an excitation filter wheel 4, and an illumination prism module 5. The field lens system 3 is correspondingly disposed below the light source module 2. The illumination prism module 5 includes a beam splitter 52, multiple reflectors 531, 532, 533, at least two Dowell prisms 541, 542, and at least two tilting reflectors 551, 552 (e.g., ...). Figure 3 (As shown). In this embodiment, the light source module 2 emits a beam L to the field lens system 3 for beam straightening and shaping, then filters it via the excitation filter wheel 4, and directs the beam L into the beam splitter 52 of the illumination prism module 5 to separate it into at least two beams L1 and L2. Multiple reflectors 531, 532, and 533 adjust the directions of the at least two beams L1 and L2, causing them to pass through at least two dovey prisms 541 and 542 respectively, and then through at least two tilting reflectors 551 and 552, so that the at least two beams L1 and L2 illuminate the illumination area 6A of the sample 6 in different directions. The detection module 7 captures the image of the sample 6, which is then analyzed by the processor 8. In this embodiment, the optical detection system is applicable to the desktop nucleic acid detection device 10, but is not limited thereto. The nucleic acid detection device 10 has a housing 100, and the optical detection system is installed inside the housing 100. Its position and the size of related components can be adjusted according to the actual configuration of the sample 6, and are not limited to the position shown in the figure. Of course, in addition to the lighting system 1 and the detection module 7, the nucleic acid detection device 10 also has other functional modules, such as: heating module (not shown), display module (not shown), etc. The lighting system 1, the detection module 7 and other functional modules are all electrically connected to the processor 8. Therefore, the processor 8 can receive relevant information transmitted by multiple functional modules, perform data calculation, analysis and processing, and then control the operation of the light source module 2, field lens system 3, excitation filter wheel 4, illumination prism module 5, detection module 7 and other functional modules of the lighting system 1. In this embodiment, after the light source module 2 emits a white light beam L, the beam L is split into two beams L1 and L2 by the illumination prism module 5. The multiple prism components inside the illumination prism module 5 adjust the travel path and direction of the beams L1 and L2 so that they converge synchronously and in the same direction on the illumination area 6A of the sample 6. This allows the sample 6 to be uniformly illuminated from multiple directions, improving the uniformity of light and reducing shadows. As a result, the detection module 7 can detect a clear, shadow-free, and noise-free sample image and transmit it to the processor 8 for image analysis and evaluation.

[0056] Please see Figure 5 . Figure 5This is a cross-sectional structural diagram of the lighting system and detection module according to a preferred embodiment of the present invention. In this embodiment, the optical detection system of the present invention is as follows: Figure 5As shown, the system includes an illumination system 1, a detection module 7, and a processor 8. When the light source module 2 of the illumination system 1 emits a white light beam L downwards, the beam L is straightened and shaped by the field lens system 3, and then filtered by the excitation filter wheel 4 to change the wavelength of the beam L, thereby adjusting it to the desired specific wavelength. The beam L's direction of travel is then changed from vertically downward to horizontal by the first reflecting mirror 51 of the illumination prism module 5. Subsequently, the beam L is split into two beams L1 and L2 by the beam splitter 52 inside the illumination prism module 5, and the paths and directions of the two beams L1 and L2 are finely adjusted by multiple reflecting mirrors 53 and at least two Doppler prisms 54. When L1 and L2 travel within the illumination prism module 5, they both travel in a generally horizontal direction. Therefore, only L1 is shown in the figure, with another beam L2 traveling horizontally on the opposite side roughly indicated. After these two beams L1 and L2 are guided into at least two tilting mirrors 55, the tilt angles of these mirrors are adjusted so that beams L1 and L2 synchronously point downwards and converge on the illumination area 6A of the sample 6, thereby exciting the sample 6 to emit specific fluorescence. The detection module 7 then detects the fluorescence beam L3 emitted by the sample 6, thus acquiring the sample image, and transmits it to the processor 8 for image analysis and evaluation. In some embodiments, the detection module 7 may include a camera (not shown), but is not limited thereto. In other embodiments, the camera includes one of a charge-coupled device (CCD), a complementary metal-oxide-semiconductor (CMOS), and other digital imaging sensors to sense the fluorescence image emitted by the aforementioned sample 6. In this embodiment, the main design of the optical detection system is dual illumination. The light beam L is split into two beams, L1 and L2, by a beam splitter 52. These two beams, L1 and L2, are then adjusted to converge on the illumination area 6A of the sample 6 from opposite directions. This illuminates the area 6A from both sides, improving light uniformity, reducing shadows, resulting in a clearer sample image, and making the detection module 7 more accurate and reliable in its detection judgment. Of course, as mentioned above, the system can also be designed with three-directional illumination or four-directional illumination, etc., depending on actual needs; it is not limited to these limitations.However, in this embodiment, the dual-illumination optical detection system guides light from the vertical direction to the horizontal direction in a compact space, splits it into two beams, and then converges them simultaneously on the sample 6 in the vertical direction, causing it to emit fluorescence. The detection module 7 then performs image extraction and detection in the vertical direction. This not only effectively increases the uniformity and clarity of light on the sample 6, as mentioned above, making the detection results more accurate and reliable, but also, through the more compact configuration of multiple optical components, effectively utilizes space and reduces the volume of the optical detection system, making the internal space utilization of the nucleic acid detection device 10 more flexible.

[0057] In summary, this invention provides an illumination system and an optical detection system suitable for nucleic acid detection devices. The illumination system includes a light source module, a field lens system, an excitation filter wheel, and an illumination prism module. The illumination prism module splits a beam from the light source module into at least two beams, providing at least two illumination paths that illuminate the sample's illumination area from two different directions. This improves the uniformity and intensity of light on the sample, resulting in more accurate and reliable detection results. Furthermore, the excitation filter wheel filters the white light emitted by the light source module, allowing beams of specific wavelengths to pass through, thereby limiting the illumination spectrum and reducing unnecessary light entering the illumination prism module, thus reducing noise during detection. Additionally, the illumination system of this invention has the advantage of compact configuration, reducing the overall size of the optical detection system and making space utilization more flexible.

Claims

1. A lighting system suitable for a nucleic acid detection device, comprising: One light source module; A mirror system is correspondingly set under this light source module; One excitation filter wheel; and An illumination prism module comprising: a beam splitter, multiple mirrors, at least two Dowell prisms, and at least two tilting mirrors; in, The light source module emits a light beam to the field mirror system for beam straightening and shaping, then filters it through the excitation filter wheel, and the light beam enters the beam splitter of the illumination prism module to be separated into at least two beams. The directions of the at least two beams are adjusted by the multiple reflectors so that they pass through the at least two Dowell prisms respectively, and then through the at least two tilting reflectors so that the at least two beams illuminate a sample in different directions.

2. The lighting system of claim 1, wherein the light source module is a white light source module and includes a laser-driven white light source kit.

3. The illumination system of claim 1, wherein the field mirror system has a movable prism for beam collimation and shaping.

4. The lighting system of claim 1, wherein the excitation filter wheel is disposed between the light source module and the lighting prism module for filtering light and allowing light of a specific wavelength to pass through.

5. The lighting system of claim 1, wherein the lighting prism module further includes a first reflector, correspondingly disposed between the excitation filter wheel and the beam splitter, for changing the direction of the light beam and guiding the light beam into the beam splitter.

6. The lighting system of claim 1, wherein the plurality of reflectors are a plurality of elliptical plane mirrors for guiding the at least two beams of light into the at least two Dove prisms.

7. The lighting system of claim 1, wherein the interior of the at least two Dove prisms is coated with a Vis0° coating for fine-tuning the orientation of the at least two beams.

8. The lighting system of claim 1, wherein the at least two tilting mirrors are respectively disposed on a mirror base, and each mirror base is provided with a plurality of fine adjustment screws for precisely adjusting a tilt angle of the at least two tilting mirrors.

9. The lighting system of claim 1, wherein the number of the at least two Dove prisms, the at least two tilting mirrors, and the at least two beams are all two, that is, the light source module emits the beam to the beam splitter to separate it into two beams, and the directions of the two beams are adjusted by the plurality of mirrors so that they pass through the two Dove prisms respectively, and then through the two tilting mirrors, so that the two beams illuminate the lighting area of ​​the sample in different directions to achieve dual lighting.

10. An optical detection system suitable for a nucleic acid detection device, the optical detection system comprising: A lighting system, including One light source module; A mirror system is correspondingly set under this light source module; One excitation filter wheel; and An illumination prism module comprising: a beam splitter, multiple mirrors, at least two Dowell prisms, and at least two tilting mirrors; A detection module; and One processor; in, The light source module emits a beam of light to the field lens system for beam straightening and shaping, then filters it through the excitation filter wheel, and the beam enters the beam splitter of the illumination prism module to separate it into at least two beams. The directions of the at least two beams are adjusted by the multiple reflectors so that they pass through the at least two Dowell prisms respectively, and then through the at least two tilting reflectors so that the at least two beams illuminate a sample area in different directions. The detection module then captures an image of the sample, which is then analyzed by the processor.

11. The optical detection system of claim 10, wherein the light source module is a white light source module and includes a laser-driven white light source kit.

12. The optical detection system of claim 10, wherein the field mirror system has a movable prism for beam collimation and shaping.

13. The optical detection system of claim 10, wherein the excitation filter wheel is disposed between the light source module and the illumination prism module for filtering light and allowing light of a specific wavelength to pass through.

14. The optical inspection system of claim 10, wherein the inspection module includes a camera, and the camera includes one of a charge-coupled device, a complementary metal-oxide-semiconductor (CMOS) sensor, and other digital imaging sensors.

Images