A pre-prepared food bacteria colony detection device and a method of using the same

Abstract

This invention belongs to the field of food testing technology, specifically a pre-prepared vegetable microbial colony detection device and its usage method. It includes a platform and a sample placement section located on top of the platform, and further includes: a separation chamber containing a separation cylinder for holding the sample; and an aeration component, located inside or connected to the separation chamber, for generating microbubbles in the sample liquid to guide oil to the surface. This invention uses the aeration component to generate microbubbles to guide oil to the surface, and then uses a liftable oil-absorbing component to remove the oil, achieving oil-water separation and eliminating interference from oil in microbial colony detection. A primary filter chamber traps impurities under low pressure, and a secondary filter chamber breaks down and traps dead bacteria under medium pressure, allowing live bacteria to pass through and enter the detection tray for testing, thereby improving detection accuracy. This invention has a simple structure, high degree of automation, accurate and reliable detection results, and is convenient to use.

Description

Technical Field

[0001] This invention relates to the field of food testing technology, and in particular to a pre-prepared vegetable bacterial colony detection device and its usage method. Background Technology

[0002] Prepared meals, as an important product of modern food industry, have gradually become an important part of catering and home consumption due to their convenience and efficiency. However, prepared meals are susceptible to microbial contamination during processing, storage, and transportation, with total bacterial count being one of the key indicators for measuring their hygienic quality. Therefore, accurate and efficient bacterial count testing of prepared meals is of great significance for ensuring food safety.

[0003] However, existing detection technologies still have the following shortcomings in practical applications: Prepared dishes typically contain a certain proportion of oil, especially in liquid or semi-solid prepared dishes such as soups and sauces, where oil and water form an emulsion. Existing detection methods usually directly test the sample without effectively separating the oil. The presence of oil can interfere with bacterial colony cultivation and detection, leading to low test results or false negatives, thus affecting the accuracy of the test. Although some existing technologies use centrifugation or static stratification to separate oil, these methods are cumbersome, inefficient, and difficult to automate for continuous detection.

[0004] Therefore, how to achieve oil-water separation during the bacterial colony detection process of prepared vegetables in order to improve the accuracy of detection is a technical problem that urgently needs to be solved in this field. Summary of the Invention

[0005] In view of the deficiencies in the existing technology, the purpose of this invention is to provide a pre-cooked vegetable colony detection device and its usage method, which can effectively separate the oil and water phase in the pre-cooked vegetable sample, eliminate the interference of oil on colony detection, and improve the detection accuracy.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A pre-prepared vegetable colony detection device includes a platform and a sample placement section disposed on top of the platform, and further includes:

[0008] A separation chamber, wherein a separation cylinder for containing the sample to be tested is fixedly installed inside the separation chamber;

[0009] An aeration component, located inside or connected to the separation chamber, is used to generate microbubbles in the sample liquid to guide the oil to the liquid surface.

[0010] An oil-absorbing assembly is disposed above or inside the separation cylinder. The oil-absorbing assembly includes a liftable oil-absorbing component. The suction port of the oil-absorbing component is located at the liquid surface when it is in the descending state, and is used to remove the oil from the liquid surface so that the colonies are enriched in the aqueous phase.

[0011] The primary filter box has its inlet connected to the outlet of the separation box and is used to receive the water phase after oil-water separation. The primary filter box is equipped with a first filter element, which traps impurities under low pressure conditions.

[0012] The secondary filter box has its inlet connected to the outlet of the primary filter box and is used to receive the liquid after primary filtration. The secondary filter box is equipped with a second filter element, and under medium pressure, the second filter element breaks down and traps dead bacteria, allowing live bacteria to pass through and be retained.

[0013] The primary and secondary filter boxes are supported on the platform by the support pillars.

[0014] The detection tray is located at the outlet or inside the secondary filter box and is used to receive live bacteria that have passed through the second filter element and perform colony detection.

[0015] As a preferred technical solution of this application, it further includes a sampling mechanism, which includes a sampler, a sampling head, and a sample delivery tube. A top frame is installed on the peripheral wall of the separation chamber. The sampling head is located below the top frame and is vertically aligned with the top of the sample placement section for contacting the sample to be tested placed on the sample placement section. The sampler is fixed to the bottom of the top frame and is connected to one end of the sampling head and one end of the sample delivery tube. The other end of the sample delivery tube is connected to the inlet of the separation chamber for conveying the sample to the separation chamber.

[0016] As a preferred technical solution of this application, the aeration assembly includes an aeration pump and an aeration pipe. The aeration pipe is disposed inside the separation tank, and the air outlet of the aeration pipe is located at the bottom or lower part of the separation cylinder. The aeration pump is disposed outside the separation tank and is connected to the aeration pipe through a pipeline.

[0017] As a preferred technical solution of this application, the oil suction assembly further includes an oil suction pump and an oil storage tank. The oil suction component is vertically and flexibly disposed above the separation cylinder. The suction port of the oil suction component is positioned facing the liquid surface inside the separation cylinder. The oil suction component is connected to the inlet of the oil suction pump through an oil suction pipe, and the outlet of the oil suction pump is connected to the oil storage tank.

[0018] As a preferred technical solution of this application, a first drain pipe is provided between the outlet of the separation box and the inlet of the primary filter box, and a second drain pipe is provided between the outlet of the primary filter box and the inlet of the secondary filter box. A support is fixed inside the first drain pipe, and a rotating rod is rotatably provided at the bottom of the support. A power paddle is fixed inside the first drain pipe on the outer wall of the rotating rod to convert the flow force of the liquid passing through the first drain pipe into power.

[0019] As a preferred technical solution of this application, a primary guide plate and a secondary guide plate are suspended inside the primary filter box and the secondary filter box, and a transmission cylinder is provided on the primary guide plate and the secondary guide plate respectively;

[0020] The transmission cylinder is a first transmission cylinder and a second transmission cylinder. One end of the rotating rod passes through the first transmission cylinder and extends into the second transmission cylinder. The rotating rod is provided with two baffles corresponding to the upper parts of the first transmission cylinder and the second transmission cylinder, respectively, for guiding and distributing the liquid to the first filter element and the second filter element.

[0021] As a preferred technical solution of this application, the first filter element is a primary microporous filter membrane disc, which is horizontally arranged in the primary bacterial filter box, dividing the primary bacterial filter box into an upper chamber and a lower chamber. A pressure pump is provided on the top of the primary bacterial filter box, and the pressure pump is connected to the upper chamber to create a low-pressure environment in the primary bacterial filter box.

[0022] The second filter element is a two-stage microporous filter membrane disc, which is horizontally arranged inside the two-stage bacterial filtration chamber, dividing the two-stage bacterial filtration chamber into an upper chamber and a lower chamber. A pressure pump is provided on the top of the two-stage bacterial filtration chamber, and the pressure pump is connected to the upper chamber to create a medium-pressure environment inside the two-stage bacterial filtration chamber.

[0023] As a preferred technical solution of this application, the primary and secondary filter chambers are further provided with an anti-clogging vibration assembly. The anti-clogging vibration assembly includes a turntable, multiple first convex balls, multiple second convex balls, multiple telescopic rods, and multiple springs. The turntable is fixed on the rotating rod and located inside the transmission cylinder. The first convex balls are disposed on the outer peripheral wall of the turntable, and the second convex balls are disposed on the inner peripheral wall of the transmission cylinder and intermittently abut against the first convex balls. The telescopic rods are arranged in a circumferential array between the primary microporous filter membrane disc and the inner wall of the primary filter chamber. The springs are sleeved on the telescopic rods and are used to drive the primary microporous filter membrane disc to generate intermittent vibration when the turntable rotates.

[0024] As a preferred technical solution of this application, an electric push rod is provided inside the separation cylinder. The electric push rod is fixed to the top or inner wall of the separation cylinder, and the movable end of the electric push rod is connected to the oil suction component to drive the oil suction component to rise and fall.

[0025] A method for using the pre-prepared vegetable microbial colony detection device as described above includes the following steps:

[0026] The sample to be tested is placed in the sampling section, and the sample is transported to the separation cylinder in the separation chamber by the sampling mechanism;

[0027] S1: Microbubbles are generated in the sample liquid through the aeration component, which guides the oil to the liquid surface;

[0028] S2: The oil on the surface of the liquid is removed by the oil-absorbing component, so that the colonies are enriched in the aqueous phase;

[0029] S3: The aqueous phase after oil-water separation is introduced into the primary filter box, where impurities are intercepted by the first filter element under low pressure.

[0030] S4: The liquid after primary filtration is introduced into the secondary filtration chamber, where it passes through the second filter element under medium pressure to break down and trap dead bacteria, allowing live bacteria to pass through and remain.

[0031] S5: Perform colony detection on live bacteria using a detection tray.

[0032] The pre-prepared vegetable microbial colony detection device and its usage method described in this invention have the following beneficial effects:

[0033] 1. This solution achieves effective separation of oil and water in the sample through aeration and oil absorption components, avoiding interference from oil on bacterial colony detection and improving detection accuracy;

[0034] 2. This solution improves detection accuracy by setting up a primary filter chamber to trap impurities under low pressure and a secondary filter chamber to break down and trap dead bacteria under medium pressure, so that the liquid entering the detection tray contains only live bacteria.

[0035] 3. This solution uses a propeller and rotating rod installed in the drain pipe to drive the anti-clogging vibration component with the kinetic energy of the liquid flow. This causes the microporous filter membrane disc to vibrate intermittently during the filtration process, effectively preventing microporous clogging and ensuring the continuous stability of the filtration process. Attached Figure Description

[0036] The present invention includes the following figures:

[0037] The accompanying drawings are provided to better understand the invention and are not intended to unduly limit the scope of the invention. Wherein:

[0038] Figure 1 This is a schematic diagram of the structure of a pre-prepared vegetable colony detection device and its usage method according to the present invention.

[0039] Figure 2 This is a slanted view of the pre-prepared vegetable colony detection device and its usage method described in this invention.

[0040] Figure 3 This is a schematic diagram of the components inside the primary and secondary filter boxes of the pre-prepared vegetable colony detection device and its usage method described in this invention.

[0041] Figure 4This is a bottom view of the components inside the primary and secondary filter boxes of the pre-prepared vegetable colony detection device and its usage method described in this invention.

[0042] Figure 5 This is a cross-sectional view of the structure between the first drain pipe and the transmission cylinder of the pre-prepared vegetable colony detection device and its usage method described in this invention.

[0043] Figure 6 This is a cross-sectional view of the separation cylinder of the pre-prepared vegetable colony detection device and its usage method described in this invention.

[0044] Figure 7 This is a top-section schematic diagram of the separation cylinder of the pre-prepared vegetable colony detection device and its usage method described in this invention.

[0045] Figure 8 This is a schematic diagram of the oil-absorbing plate, oil-absorbing head, and notched portion of the pre-cooked vegetable colony detection device and its usage method described in this invention.

[0046] The correspondence between the numbers in the attached diagram is as follows:

[0047] 1-Platform; 101-Sampling section; 102-Controller; 2-Top frame; 201-Hydraulic cylinder; 202-Sampler; 203-Sampling head; 204-Sample delivery tube; 3-Separation box; 301-Separation cylinder; 302-Aeration pump; 303-Aeration pipe; 304-Electric push rod; 305-Oil suction plate; 3050-Oil suction head; 3051-Notch; 306-Oil suction pump; 307-Oil storage tank; 308-First drain pipe; 309-Solenoid valve; 4-Support; 401-Rotation Rod; 402-Power propeller; 5-First-stage filter box; 501-First transmission cylinder; 5010-Baffle; 502-Turntable; 503-First convex ball; 504-Second convex ball; 505-First-stage guide plate; 506-Fixing rod; 507-First-stage microporous filter membrane plate; 508-Telescopic rod; 509-Spring; 510-Second drain pipe; 6-Second-stage filter box; 601-Second-stage guide plate; 602-Second-stage microporous filter membrane plate; 603-Detection plate; 7-Support column; 701-Pressure pump. Detailed Implementation

[0048] The present invention will be further described in detail below with reference to the accompanying drawings. This detailed description is an illustration in conjunction with exemplary embodiments of the invention, including various details of the embodiments to aid understanding, and should be considered merely exemplary. Therefore, those skilled in the art will recognize that various changes and modifications can be made to the embodiments described herein without departing from the scope and spirit of the invention. Similarly, for clarity and brevity, descriptions of well-known functions and structures are omitted in the following description.

[0049] This invention provides a pre-prepared food bacterial colony detection device and its usage method, applicable to the quality control stage of pre-prepared food production, particularly suitable for detecting the total bacterial count in liquid or semi-solid pre-prepared foods (such as soups, sauces, stews, etc.). During the production, storage, and transportation of pre-prepared foods, oil and water often coexist. Traditional detection methods suffer from inaccurate results due to oil interference and difficulty in distinguishing between live and dead bacteria, affecting the reliability of hygiene quality assessment. This device integrates oil-water separation, graded filtration, and live bacteria detection functions, enabling accurate detection of live bacteria in pre-prepared food samples. It is widely used in on-site or laboratory testing by food processing enterprises, third-party testing institutions, and food safety regulatory departments.

[0050] The embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. The drawings are schematic structural diagrams, and the actual dimensions can be adjusted appropriately according to usage requirements. It should be noted that the specific components used in this embodiment (such as sampler type, aeration pipe structure, oil suction plate form, microporous filter membrane disc specifications, etc.) are only preferred embodiments. In addition, those skilled in the art, based on their understanding of the technical solution of the present invention, can use other alternative components that can achieve the same function, and all such alternatives fall within the protection scope of the present invention.

[0051] like Figure 1 , Figure 2 As shown, a pre-prepared vegetable colony detection device includes a platform 1, a separation chamber 3, a primary filter chamber 5, a secondary filter chamber 6, and a detection tray 603.

[0052] The top of the stage 1 is provided with a sample placement section 101 for placing a sample tube containing the sample to be tested. The stage 1 is also provided with a controller 102 for controlling the operation of various actuators of the equipment. The controller 102 is electrically connected to all electric and pneumatic components of the equipment to achieve centralized control.

[0053] The separation chamber 3 is mounted on the platform 1. A separation cylinder 301 is fixed inside the separation chamber 3 to hold the sample to be tested. A top frame 2 is fixedly mounted on the peripheral wall of the separation chamber 3 by a flange bolt assembly. A sampling mechanism is provided below the top frame 2. The sampling mechanism includes a sampler 202, a sampling head 203, and a sample delivery tube 204. The sampling head 203 is perpendicular to the top of the sample placement section 101 and is used to contact the sample to be tested. The sampler 202 is fixed at the bottom of the top frame 2 and has an automatic quantitative function. The sampler 202 is connected to one end of the sampling head 203 and one end of the sample delivery tube 204. The other end of the sample delivery tube 204 is connected to the inlet of the separation chamber 3 and is used to transport a quantitative sample to the separation cylinder 301. The end of the sample delivery tube 204 extends into the separation cylinder 301 to the lower part of the separation cylinder 301 and does not contact the bottom of the separation cylinder 301. In addition, the sampler 202 can also be a manual quantitative sampling device, or other forms of automatic sampling equipment such as peristaltic pumps and metering pumps.

[0054] like Figure 1 , Figure 5 As shown, the separation cylinder 301 is equipped with an aeration assembly and an oil suction assembly. The aeration assembly includes an aeration pump 302 and an aeration pipe 303. The aeration pipe 303 is located inside the separation chamber 3, and its outlet is located at the bottom or lower part of the separation cylinder 301. The aeration pump 302 is located outside the separation chamber 3 and is connected to the aeration pipe 303 via a pipeline. It is used to generate microbubbles in the sample liquid to guide the oil to the liquid surface. Alternatively, the aeration assembly can also be a microporous aeration disc, aeration stone, or other gas dispersion device capable of generating microbubbles; the aeration pump 302 can also be replaced with a compressed air source or a nitrogen source, as long as it can generate a sufficient number of microbubbles in the liquid.

[0055] The oil suction assembly includes an oil suction element (in this embodiment, an oil suction plate 305), an electric push rod 304, an oil suction pump 306, and an oil storage tank 307. The electric push rod 304 is fixed to the top center of the separator 301 by flange bolts, and its movable end is fixedly connected to the oil suction element by bolts, used to drive the oil suction element to rise and fall. The outer diameter of the oil suction plate 305 is slightly smaller than the inner diameter of the separator 301, and the gap between it and the inner wall of the separator 301 is 1-2 mm. Multiple oil suction heads 3050 are provided at the bottom of the oil suction plate 305. The suction inlet of the oil suction head 3050 is an annular slit structure, and the suction inlet of the oil suction head 3050 is oriented towards the liquid surface inside the separator 301. The oil suction plate 305 is sealed to the inlet of the oil suction pump 306 via an oil suction pipe. The oil suction pipe passes through the side wall of the separation tank 3 and is sealed by a sealing ring. The oil suction pump 306 is fixed to the platform 1 by bolts. The oil storage tank 307 is fixed to the outer side wall of the separation tank 3 by a bracket. The outlet of the oil suction pump 306 is sealed to the oil storage tank 307. When microbubbles guide the grease to the liquid surface, the electric push rod 304 drives the oil suction plate 305 to descend until the oil suction head 3050 contacts the liquid surface. The oil suction pump 306 starts, sucking up the grease and storing it in the oil storage tank 307, so that the colonies are enriched in the aqueous phase. In addition, the oil suction component can be in the form of an oil suction pipe, oil suction cotton, or oil suction roller; the electric push rod 304 can also be replaced by a linear drive device such as a cylinder, hydraulic cylinder, or lead screw mechanism; the oil suction pump 306 can be a diaphragm pump, peristaltic pump, or other types of fluid transfer pump.

[0056] like Figure 1 , Figure 3 , Figure 4As shown, a first drain pipe 308 is installed between the bottom outlet of the separation chamber 3 and the inlet of the primary filter chamber 5, and a second drain pipe 510 is installed between the outlet of the primary filter chamber 5 and the inlet of the secondary filter chamber 6. A solenoid valve 309 is installed on the first drain pipe 308, and the solenoid valve 309 is electrically connected to the controller 102, which controls its on / off state to control the flow of liquid. A support 4 is fixed inside the first drain pipe 308, and a rotating rod 401 is rotatably installed at the bottom of the support 4. A propeller 402 is fixed inside the first drain pipe 308 on the outer wall of the rotating rod 401, which is used to convert the flow force of the liquid passing through the first drain pipe 308 into power. The second drain pipe 510 is equipped with a support, rotating rod, and propeller with the same structure as the first drain pipe 308. One end of the rotating rod 401 extends sequentially through the primary filter chamber 5 and the second drain pipe 510 into the secondary filter chamber 6. Sealed bushings are provided at the positions where the rotating rod 401 passes through the walls of each chamber. The end of the rotating rod 401 inside the secondary filter chamber 6 is rotatably connected to the bottom of the secondary filter chamber 6 through a bearing, so as to achieve stable support for the rotating rod 401. In addition, the propeller 402 can adopt an impeller or turbine structure; the rotating rod 401 can be supported by bearings or bushings; the solenoid valve 309 can be replaced by an electric valve or a pneumatic valve or other valves with on / off control functions.

[0057] Inside the primary filter chamber 5 and the secondary filter chamber 6, a primary guide plate 505 and a secondary guide plate 601 are suspended by welding to the inner wall of the chamber via connecting rods. The vertical distance between the primary guide plate 505 and the secondary guide plate 601 and the corresponding filter element in the filter chamber is 5-8 cm. A first transmission cylinder 501 and a second transmission cylinder (not numbered in the attached drawing, but with the same structure and dimensions as the first transmission cylinder 501) are welded onto the primary guide plate 505 and the secondary guide plate 601, respectively. A rotating rod 401 passes through the inside of the first transmission cylinder 501 and the second transmission cylinder, without contacting the cylinder wall. Two baffles 5010 corresponding to the upper part of the first transmission cylinder 501 and the second transmission cylinder are welded onto the rotating rod 401 to guide and distribute the liquid to the first filter element and the second filter element. Both the primary filter chamber 5 and the secondary filter chamber 6 are supported on the platform 1 by support columns 7.

[0058] The first filter element is a primary microporous filter membrane disc 507 with a pore size of 0.8 μm. The primary microporous filter membrane disc 507 is horizontally arranged inside the primary bacterial filter chamber 5, dividing the primary bacterial filter chamber 5 into an upper chamber and a lower chamber. A pressure pump 701 is sealed on the top of the primary bacterial filter chamber 5 through a flange interface. The pressure pump 701 is connected to the upper chamber and is equipped with a pressure regulating valve for precise control of the pressure inside the chamber, thereby creating a low-pressure environment (in this embodiment, the low pressure is -0.02 MPa to -0.05 MPa) inside the primary bacterial filter chamber 5 to facilitate the passage of liquid through the primary microporous filter membrane disc 507. At the same time, the primary microporous filter membrane disc 507 is used to trap large particulate impurities (such as solid impurities much larger than the size of bacteria and non-target particles). The second filter element is a secondary microporous membrane disc 602 with a pore size of 0.22 μm. The secondary microporous membrane disc 602 is horizontally positioned within the secondary bacterial filtration chamber 6, dividing the chamber into an upper and lower chamber. A pressure pump 701 is sealed to the top of the secondary bacterial filtration chamber 6 via a flange interface. The pressure pump 701 is connected to the upper chamber and is equipped with a pressure regulating valve for precise pressure control within the chamber, creating a medium-pressure environment (0.2 MPa to 0.5 MPa in this embodiment) within the secondary bacterial filtration chamber 6. Under medium-pressure conditions, dead bacteria in the liquid are broken down and retained by the secondary microporous membrane disc 602, while live bacteria can pass through. In addition, the first and second filter elements can be ceramic filter membranes, metal filter screens, or other filter media with microporous filtration function; the pressure pump 701 can be replaced with a vacuum pump or negative pressure generator; the pressure values ​​of low-pressure and medium-pressure environments can be adjusted according to the actual filter membrane pore size and liquid properties, and are not limited to the range given in this embodiment.

[0059] like Figure 3 , Figure 4 and Figure 5As shown, the primary filter box 5 and the secondary filter box 6 are also equipped with anti-clogging vibration components. These components include a turntable 502, multiple first convex balls 503, multiple second convex balls 504, multiple telescopic rods 508, and multiple springs 509. One set of anti-clogging vibration components is installed in each of the first and second transmission cylinders. Each set includes one turntable 502, eight first convex balls 503, eight second convex balls 504, six telescopic rods 508, and six springs 509. The turntable 502... The key is fixed on the rotating rod 401 and located inside the transmission cylinder. Eight first convex balls 503 are evenly welded to the outer peripheral wall of the turntable 502, and eight second convex balls 504 are evenly welded to the inner peripheral wall of the transmission cylinder, and intermittently abut against the first convex balls 503. Six telescopic rods 508 are evenly arranged in a circumferential array between the primary microporous filter membrane disc 507 and the inner wall of the primary bacterial filter box 5, and between the secondary microporous filter membrane disc 602 and the inner wall of the secondary bacterial filter box 6. Springs 509 are sleeved on the telescopic rods 508. When the liquid flows through the first drain pipe 308, the liquid's flow force drives the propeller 402 to rotate. The propeller 402 drives the rotating rod 401 to rotate, which in turn drives the turntable 502 to rotate. The first convex ball 503 then performs circular motion and intermittently squeezes the second convex ball 504, causing the second convex ball 504 to displace. This, in turn, causes the transmission cylinder to vibrate. Through the cooperation of the telescopic rod 508 and the spring 509, the primary microporous filter membrane disc 507 and the secondary microporous filter membrane disc 602 produce intermittent multi-directional vibrations, preventing clogging during the filtration process. Alternatively, the first convex ball 503 and the second convex ball 504 can employ other intermittent drive structures such as a cam and roller combination or an eccentric wheel and push rod combination; the telescopic rod 508 and the spring 509 can employ other elastic support structures such as elastic brackets or rubber buffers; and the vibration generation method can also use alternative solutions such as ultrasonic vibrators or mechanical vibrators.

[0060] like Figure 3 As shown, the detection tray 603 is located in the lower chamber of the secondary filtration chamber 6, below the secondary microporous membrane tray 602, and is used to receive live bacteria passing through the secondary microporous membrane tray 602. The detection tray 603 integrates a prior art colony detector, which can automatically detect the total number of live bacteria in the liquid and transmit the detection results to the controller 102. In addition, the detection tray 603 can employ colony detection devices based on different principles, such as ATP bioluminescence detectors, impedance detectors, or optical detectors, or it can take the form of replaceable test strips or petri dishes for manual reading of results.

[0061] like Figure 8As shown, the bottom of the oil suction plate 305 is provided with multiple oil suction heads 3050. Two notches 3051 are opened on the oil suction head 3050. When the sample delivery pipe 204 and the aeration pipe 303 are located in the separation cylinder 301, they correspond to the two notches 3051 respectively, so that when the oil suction plate 305 rises and falls, it will not affect the sample delivery pipe 204 and the aeration pipe 303. In addition, when it is necessary to add more pipes in the separation cylinder 301, the number of notches 3051 can be increased accordingly.

[0062] It should be noted that the detection tray 603 can be a drawer-type device installed on the secondary filter box 6. The detection tray 603 is slidably connected to the secondary filter box 6 via a slide rail, and a silicone sealing strip is provided at the contact gap between the detection tray 603 and the secondary filter box 6 to ensure airtightness. Transparent glass windows can be added to the separation box 3, the primary filter box 5, and the secondary filter box 6. The transparent glass is fixed and sealed to the box window by a sealing strip. The separation cylinder 301 is also provided with a transparent window corresponding to the transparent window on the separation box 3. The transparent windows of the two are completely aligned, and the alignment error does not exceed 1mm.

[0063] Example 2: Equipment Usage Method

[0064] The usage method of the embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0065] First, place the sample tube containing the pre-prepared food sample to be tested (such as pre-prepared soup) on the sample placement part 101 of the stage 1, so that the sample tube is directly below the sampling head 203.

[0066] When the equipment is started, the controller 102 controls the sampler 202 to work. The sampler 202 draws a quantitative amount of the sample to be tested (e.g., 5 mL) through the sampling head 203 and delivers the sample to the separation cylinder 301 inside the separation chamber 3 through the sample delivery tube 204. In addition, the sampling volume can be set to any value between 1 mL and 10 mL according to the detection requirements, or a continuous injection method can be used.

[0067] Next, the controller 102 starts the aeration pump 302, which aerates the sample liquid in the separation cylinder 301 through the aeration pipe 303, generating a large number of microbubbles in the liquid. The microbubbles guide the oil at the bottom of the liquid to the surface, causing the oil to float. In addition, the aeration time can be set from 30 seconds to 5 minutes depending on the oil content of the sample, or intermittent aeration can be used to enhance the oil-water separation effect.

[0068] Simultaneously, controller 102 activates electric push rod 304, driving oil suction plate 305 downwards until oil suction head 3050 contacts the liquid surface. When microbubbles carry grease to the liquid surface, oil suction pump 306 activates, sucking up the oil from the microbubbles through oil suction head 3050 and transporting it to oil storage tank 307 via oil suction pipe, while the aqueous phase is temporarily stored in separation cylinder 301, thus completing oil-water separation. Furthermore, the oil suction process can be continuous or intermittent, and the suction time can be adjusted according to the thickness of the grease layer on the liquid surface.

[0069] After oil-water separation is complete, controller 102 opens solenoid valve 309, allowing the aqueous phase containing bacterial colonies to enter the primary filter chamber 5 through the first drain pipe 308. As the aqueous phase flows through the first drain pipe 308, the fluid dynamics drive the propeller 402 to rotate, which in turn drives the rotating rod 401. Simultaneously, the aqueous phase flows to the baffle 5010 on the rotating rod 401, where it is guided and diffused to the primary microporous membrane disc 507. At the same time, pressure pump 701, located at the top of the primary filter chamber 5, applies low pressure (-0.02MPa to -0.05MPa) to the primary filter chamber 5 via a pressure regulating valve, causing the aqueous phase to pass through the primary microporous membrane disc 507 under negative pressure. The primary microporous membrane disc 507 traps large particulate impurities (such as solid impurities and non-target particles), while the remaining aqueous phase containing bacterial colonies passes through and enters the lower chamber of the primary filter chamber 5.

[0070] As the liquid flows through the first drain pipe 308, the rotation of the rotating rod 401 drives the turntable 502 to rotate. The first convex ball 503 on the outer peripheral wall of the turntable 502 then makes a circular motion and intermittently squeezes the second convex ball 504 set on the inner peripheral wall of the first transmission cylinder, causing the second convex ball 504 to be displaced. Through the cooperation of the telescopic rod 508 and the spring 509, the first-stage microporous filter membrane disc 507 is driven to vibrate intermittently in multiple directions to prevent micropore blockage.

[0071] The aqueous phase after primary filtration enters the secondary filter chamber 6 through the second drain pipe 510. Similarly, as the liquid flows through the second drain pipe 510, the liquid's flow force drives the propeller 402 to rotate, guiding and distributing the liquid to the secondary microporous membrane disc 602 via the rotating rod 401 and baffle 5010. The pressure pump 701 at the top of the secondary filter chamber 6 applies medium pressure (0.2MPa~0.5MPa) to the secondary filter chamber 6 through a pressure regulating valve, causing the liquid to pass through the secondary microporous membrane disc 602 under positive pressure. During this process, dead bacteria are broken down and retained by the secondary microporous membrane disc 602, while live bacteria pass through the secondary microporous membrane disc 602 and fall directly into the detection disc 603.

[0072] The anti-clogging vibration component inside the secondary filter box 6 rotates synchronously with the rotating rod 401. The turntable 502 drives the first convex ball 503 to squeeze the second convex ball 504 on the inner peripheral wall of the second transmission cylinder, thereby causing the secondary microporous filter membrane disc 602 to vibrate intermittently to prevent clogging.

[0073] Finally, the detector in the detection tray 603 automatically detects the total number of colonies in the collected live bacteria liquid. The detected data is transmitted to the controller 102, which analyzes and processes the data and outputs the detection results, thereby obtaining accurate live bacteria colony data.

[0074] The implementation principle of the pre-prepared vegetable colony detection device and its usage method according to this application embodiment is as follows: First, the sample cylinder containing the sample to be tested is placed in the sample placement section 101. The hydraulic cylinder 201 of the controller 102 drives the sampler 202 to move downward, so that the sampling head 203 contacts the sample to be tested. The sampler 202 sucks in a certain amount of the sample to be tested through the sampling head 203 and delivers it to the separation cylinder 301 through the sample delivery pipe 204. Then, the aeration pump 302 aerates the sample liquid in the separation cylinder 301 through the aeration pipe 303, so that... A large number of microbubbles are generated in the liquid. These microbubbles guide the oil at the bottom of the liquid to the surface of the sample liquid. At the same time, the electric push rod 304 drives the oil suction plate 305 to move downward until the oil suction head 3050 contacts the liquid surface. When the microbubbles are on the liquid surface, the oil suction pump 306 sucks up the oil in the microbubbles through the oil suction head 3050 and transports it to the oil storage tank 307 through the oil suction pipe. The water is temporarily stored in the separation cylinder 301, thus achieving the effect of oil-water separation (since bacterial colonies are generally mixed in water, and it is necessary to separate oil and water to detect bacterial colonies).

[0075] After oil-water separation, the solenoid valve 309 is opened, allowing water containing bacterial colonies to enter the primary filter chamber 5 through the first drain pipe 308. During this process, the propeller 402 installed in the first drain pipe 308 rotates due to the gravity of the water. The propeller 402 drives the rotating rod 401 to rotate, and the water flows onto the baffle 5010 on the rotating rod 401, where it is guided and diffused into the microporous filter membrane disc. Simultaneously, the pressure pump at the top of the primary filter chamber 5 generates pressure within it. If the pressure inside the primary filter chamber 5 is low, the microporous filter membrane disc can remove large particles of impurities, such as solids much larger than bacteria. Impurities and non-target particles are retained, while the remaining liquid containing water and bacterial colonies passes through and enters the secondary filter chamber 6 via the second drain pipe 510. Inside the secondary filter chamber 6, the liquid is guided and distributed to the secondary microporous membrane disc 602 by a baffle 5010 connected to the rotating rod 401 above the power cylinder. A pressure pump at the top of the secondary filter chamber 6 generates medium pressure, breaking down dead bacteria in the liquid, which are then retained by the secondary microporous membrane disc 602. Live bacteria pass through the secondary microporous membrane disc 602 and fall directly into the detection disc 603, resulting in a liquid containing live bacteria free of dead bacteria and impurities, which can then be used in the detection disc 603. The detector inside automatically detects bacterial colonies in the liquid, and the detected data is transmitted to the controller 102 for more accurate data. When the liquid passes through the primary microporous filter membrane disk 507 and the secondary microporous filter membrane disk 602, the rotating rod 401 drives the turntable 502 to rotate. The first convex ball 503 on the outer peripheral wall of the turntable 502 simultaneously performs circular motion within the first transmission cylinder 501 or the second transmission cylinder, and intermittently squeezes the second convex ball 504 set on the inner peripheral wall of the first transmission cylinder 501 and the second transmission cylinder, causing displacement. This causes the first transmission cylinder 501 and the second transmission cylinder to drive the primary microporous filter membrane disk 507 and the secondary microporous filter membrane disk 602. The disc 602 intermittently shifts in multiple directions. Because the primary microporous filter disc 507 and the secondary microporous filter disc 602 are respectively arranged in a circumferential array with the inner walls of the primary filter chamber 5 and the secondary filter chamber 6, and springs 509 are sleeved on the telescopic rods 508, when the primary microporous filter disc 507 and the secondary microporous filter disc 602 drive the telescopic rods 508 to contract, they will compress the springs 509. When the springs 509 release their elasticity, they will drive the primary microporous filter disc 507 and the secondary microporous filter disc 602 to move and reset, thereby achieving the effect of vibration of the primary microporous filter disc 507 and the secondary microporous filter disc 602, preventing clogging during the filtration process.

[0076] All structures in this application can be customized in terms of material and length according to actual usage. The attached drawings are schematic structural diagrams, and the actual dimensions can be adjusted accordingly.

[0077] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0078] The above description is only a preferred embodiment of the present invention. The scope of protection of the present invention is not limited to the above embodiments. Any equivalent modifications or changes made by those skilled in the art based on the content disclosed in the present invention should be included in the scope of protection set forth in the claims.

Claims

1. A pre-prepared vegetable colony detection device, comprising a platform and a sample placement section disposed on top of the platform, characterized in that, Also includes: A separation chamber, wherein a separation cylinder for containing the sample to be tested is fixedly installed inside the separation chamber; An aeration component, located inside or connected to the separation chamber, is used to generate microbubbles in the sample liquid to guide the oil to the liquid surface. An oil-absorbing assembly is disposed above or inside the separation cylinder. The oil-absorbing assembly includes a liftable oil-absorbing component. The suction port of the oil-absorbing component is located at the liquid surface when it is in the descending state, and is used to remove the oil from the liquid surface so that the colonies are enriched in the aqueous phase. The primary filter box has its inlet connected to the outlet of the separation box and is used to receive the water phase after oil-water separation. The primary filter box is equipped with a first filter element, which traps impurities under low pressure conditions. The secondary filter box has its inlet connected to the outlet of the primary filter box and is used to receive the liquid after primary filtration. The secondary filter box is equipped with a second filter element, and under medium pressure, the second filter element breaks down and traps dead bacteria, allowing live bacteria to pass through and be retained. The primary and secondary filter boxes are supported on the platform by the support pillars. The detection tray is located at the outlet or inside the secondary filter box and is used to receive live bacteria that have passed through the second filter element and perform colony detection.

2. The pre-prepared vegetable colony detection device as described in claim 1, characterized in that: It also includes a sampling mechanism, which includes a sampler, a sampling head, and a sample delivery tube. A top frame is installed on the periphery of the separation chamber. The sampling head is located below the top frame and is perpendicular to the top of the sample placement section for contacting the sample to be tested placed on the sample placement section. The sampler is fixed to the bottom of the top frame and is connected to one end of the sampling head and one end of the sample delivery tube. The other end of the sample delivery tube is connected to the inlet of the separation chamber for transporting the sample to the separation chamber.

3. The pre-prepared vegetable colony detection device as described in claim 2, characterized in that: The aeration assembly includes an aeration pump and an aeration pipe. The aeration pipe is located inside the separation tank, and the air outlet of the aeration pipe is located at the bottom or lower part of the separation cylinder. The aeration pump is located outside the separation tank and is connected to the aeration pipe through a pipeline.

4. The pre-prepared vegetable colony detection device as described in claim 1, characterized in that: The oil suction assembly also includes an oil suction pump and an oil storage tank. The oil suction component is vertically and vertically positioned above the separation cylinder. The suction port of the oil suction component faces the liquid surface inside the separation cylinder. The oil suction component is connected to the inlet of the oil suction pump through an oil suction pipe, and the outlet of the oil suction pump is connected to the oil storage tank.

5. The pre-prepared vegetable microbial colony detection device as described in claim 1, characterized in that: A first drain pipe is provided between the outlet of the separation box and the inlet of the primary filter box, and a second drain pipe is provided between the outlet of the primary filter box and the inlet of the secondary filter box. A support is fixed inside the first drain pipe, and a rotating rod is rotatably provided at the bottom of the support. A power paddle is fixed inside the first drain pipe on the outer wall of the rotating rod to convert the flow force of the liquid passing through the first drain pipe into power.

6. The pre-prepared vegetable microbial colony detection device as described in claim 5, characterized in that: The primary and secondary filter boxes are equipped with a primary guide plate and a secondary guide plate, respectively, and a transmission cylinder is provided on the primary and secondary guide plates. The transmission cylinder is a first transmission cylinder and a second transmission cylinder. One end of the rotating rod passes through the first transmission cylinder and extends into the second transmission cylinder. The rotating rod is provided with two baffles corresponding to the upper parts of the first transmission cylinder and the second transmission cylinder, respectively, for guiding and distributing the liquid to the first filter element and the second filter element.

7. The pre-prepared vegetable microbial colony detection device as described in claim 6, characterized in that: The first filter element is a primary microporous filter membrane disc, which is horizontally arranged in the primary bacterial filter box, dividing the primary bacterial filter box into an upper chamber and a lower chamber. A pressure pump is provided on the top of the primary bacterial filter box, and the pressure pump is connected to the upper chamber to create a low-pressure environment in the primary bacterial filter box. The second filter element is a two-stage microporous filter membrane disc, which is horizontally arranged inside the two-stage bacterial filtration chamber, dividing the two-stage bacterial filtration chamber into an upper chamber and a lower chamber. A pressure pump is provided on the top of the two-stage bacterial filtration chamber, and the pressure pump is connected to the upper chamber to create a medium-pressure environment inside the two-stage bacterial filtration chamber.

8. The pre-prepared vegetable colony detection device as described in claim 6, characterized in that: The primary and secondary filter chambers are also equipped with anti-clogging vibration components. The anti-clogging vibration components include a turntable, multiple first convex balls, multiple second convex balls, multiple telescopic rods, and multiple springs. The turntable is fixed to the rotating rod and located inside the transmission cylinder. The first convex balls are disposed on the outer peripheral wall of the turntable, and the second convex balls are disposed on the inner peripheral wall of the transmission cylinder and intermittently abut against the first convex balls. The telescopic rods are arranged in a circumferential array between the primary microporous filter membrane disc and the inner wall of the primary filter chamber. The springs are sleeved on the telescopic rods and are used to drive the primary microporous filter membrane disc to generate intermittent vibration when the turntable rotates.

9. The pre-prepared vegetable colony detection device as described in claim 1, characterized in that: An electric push rod is installed inside the separation cylinder. The electric push rod is fixed to the top or inner wall of the separation cylinder. The movable end of the electric push rod is connected to the oil suction component and is used to drive the oil suction component to rise and fall.

10. A method of using the pre-prepared vegetable colony detection device as described in any one of claims 1-9, characterized in that, Includes the following steps: The sample to be tested is placed in the sampling section, and the sample is transported to the separation cylinder in the separation chamber by the sampling mechanism; S1: Microbubbles are generated in the sample liquid through the aeration component, which guides the oil to the liquid surface; S2: The oil on the surface of the liquid is removed by the oil-absorbing component, so that the colonies are enriched in the aqueous phase; S3: The aqueous phase after oil-water separation is introduced into the primary filter box, where impurities are intercepted by the first filter element under low pressure. S4: The liquid after primary filtration is introduced into the secondary filtration chamber, where it passes through the second filter element under medium pressure to break down and trap dead bacteria, allowing live bacteria to pass through and remain. S5: Perform colony detection on live bacteria using a detection tray.

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