Major gene bHLH150 for controlling cold resistance of rice and application of major gene bHLH150

By cloning and applying the major cold-resistant gene bHLH150 in rice, the problem of rice being susceptible to cold damage at low temperatures has been solved, resulting in a significant improvement in cold resistance. This provides a breeding method for molecular marker-assisted selection and promotes the development of cold-resistant rice varieties.

CN122303252APending Publication Date: 2026-06-30GUANGXI UNIV
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Patent Information

Application Number
CN202610478318.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-13
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Rice is susceptible to chilling injury under low-temperature conditions. Current technologies lack effective cold-resistant gene resources, leading to yield losses and posing challenges to the breeding of cold-resistant rice varieties.

Method used

Cloning and utilizing the major cold-resistant gene bHLH150 in rice and its encoded protein, and introducing it into cold-sensitive varieties through transgenic methods to improve their cold resistance, combined with molecular marker-assisted selection breeding.

Benefits of technology

It significantly improves the survival rate and cold resistance of rice at low temperatures, providing a theoretical basis and practical means for the breeding of cold-resistant rice varieties, and has broad prospects for agricultural application.

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Abstract

The invention discloses a major gene bHLH150 for controlling cold resistance of rice and application of the major gene bHLH150, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the major gene bHLH150 for controlling cold resistance is shown as SEQ ID No: 1, after the gene is subjected to CRISPR / Cas9 editing mutation in rice, the cold resistance of a transgenic plant is remarkably reduced, and the transgenic plant becomes cold sensitive; the rice expressing or overexpressing the gene bHLH150 is high in survival rate at low temperature, and the cold resistance is improved. The cold-resistant major gene bHLH150 disclosed by the invention has important theoretical and practical significance on research on a rice cold-resistant regulation and control molecular mechanism and cultivation of a new rice cold-resistant variety. Wide application space and market prospects are realized in the agricultural field.
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