A major gene controlling cold tolerance in rice, bHLH150, and its application.

By cloning and applying the major cold-resistant gene bHLH150 in rice, the problem of rice being susceptible to cold damage at low temperatures has been solved, resulting in a significant improvement in cold resistance. This provides a breeding method for molecular marker-assisted selection and promotes the development of cold-resistant rice varieties.

CN122303252APending Publication Date: 2026-06-30GUANGXI UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI UNIV
Filing Date
2026-04-13
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Rice is susceptible to chilling injury under low-temperature conditions. Current technologies lack effective cold-resistant gene resources, leading to yield losses and posing challenges to the breeding of cold-resistant rice varieties.

Method used

Cloning and utilizing the major cold-resistant gene bHLH150 in rice and its encoded protein, and introducing it into cold-sensitive varieties through transgenic methods to improve their cold resistance, combined with molecular marker-assisted selection breeding.

Benefits of technology

It significantly improves the survival rate and cold resistance of rice at low temperatures, providing a theoretical basis and practical means for the breeding of cold-resistant rice varieties, and has broad prospects for agricultural application.

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Abstract

This invention discloses a major gene for controlling cold tolerance in rice. bHLH150 This invention relates to the field of plant genetic engineering technology, specifically the major gene for controlling cold tolerance. bHLH150 The nucleotide sequence is shown in SEQ ID No:1. After CRISPR / Cas9 editing mutations in rice, the transgenic plants exhibited significantly reduced cold tolerance and became cold-sensitive; while those expressing or overexpressing the gene... bHLH150 The rice exhibits a high survival rate at low temperatures, thus improving its cold resistance. This invention relates to the major cold-resistance gene. bHLH150 Research on the molecular mechanisms regulating cold tolerance in rice is of significant theoretical and practical importance for the breeding of new cold-tolerant rice varieties. It also has broad application prospects and a promising market outlook in the agricultural field.
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