Green and efficient bioreduction method using quinone-based natural compounds for textile dyeing

Abstract

This invention discloses a green and efficient bioreduction method for quinone-structured natural compounds used in textile dyeing. This method utilizes biosensor-regulated electrode microbial communities of *Shewanella putrefactiveis* and *Saccharomyces cerevisiae* as whole-cell biocatalytic reduction staining conditions. It constructs an electron transport system centered on cable bacteria and redox factors, and a dispersed staining system of quinone-structured natural compounds acting as electron acceptors. This provides a green and efficient bioreduction method for quinone-structured natural compounds used in textile dyeing. Electrons generated by biosensor-regulated electrode microbial communities are directly transferred between microbial species to cable bacteria and redox factors, and then efficiently transferred to highly dispersed natural compounds acting as electron acceptors, resulting in novel structures with dyeing properties.

Description

Technical Field

[0001] This invention relates to a green and efficient bioreduction method for natural quinone compounds used in textile dyeing, belonging to the field of textile printing and dyeing technology. Background Technology

[0002] Natural dyes are a class of dyes extracted from natural plant, animal, or microbial resources that possess color. Compared to widely used synthetic dyes, they have advantages such as wider availability, renewable resources, good environmental compatibility, and low toxicity. Quinone natural dyes are a class of natural organic compounds with conjugated quinone structures, widely found in plants, insects, and certain microorganisms. Due to their rich colors, natural sources, and environmental friendliness, these dyes have significant application value in textile dyeing. Currently, the most commonly used dyeing methods...

[0003] The limited availability of dyeing methods for natural quinone compounds in textiles greatly restricts their application. Currently, the most common dyeing method is mordant dyeing, which uses mordants to enhance the chemical reaction between dye and fiber. While this method can significantly improve dye depth and fastness, mordants remain in the dyeing wastewater, posing certain hazards to the environment and human health. Furthermore, improper handling during post-dyeing finishing can leave harmful substances on the fabric, impacting the health of users.

[0004] Therefore, it is of great significance to develop a dyeing method that uses fewer chemical reagents and is more efficient, in order to address the pollution problems existing in current dyeing methods for textiles using quinone natural compounds. Summary of the Invention

[0005] The technical problem this invention aims to solve is to address the environmental pollution and other issues associated with existing quinone dyeing methods, and to provide a dyeing method that is universally applicable to natural quinone compounds. Based on the unique chemical structure of quinones, this invention provides a green and efficient bioreduction method for natural quinone compounds used in textile dyeing. This method includes the selection of biosensor-regulated electrode microbial communities as electron donors, the construction of an electron transfer system centered on cable bacteria and redox factors, and the preparation of a dispersion system of natural quinone compounds as electron acceptors. The green and efficient bioreduction method for natural quinone compounds used in textile dyeing provided by this invention directly transfers electrons generated by biosensor-regulated electrode microbial communities to cable bacteria and redox factors via interspecies electron transfer, and then efficiently transfers these electrons to highly dispersed natural compounds as electron acceptors, resulting in a new structure with dyeing properties.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] A green and efficient bioreduction method for dyeing textiles using quinone-based natural compounds involves first mixing peptone, yeast extract, and sodium chloride in sterile water and then sterilizing the mixture to obtain a culture medium. Shewanella (ATCC 49138) and Saccharomyces cerevisiae (ATCC 9763) are inoculated separately into the sterilized culture medium cooled to room temperature, and cultured to obtain a microbial inoculum. The well-dispersed quinone natural compounds are added to a proportionally mixed electrode microbial inoculum. Electrons generated by the electrode microbial inoculum are transferred between microbial species and reduced by redox factors, resulting in the reduction of the highly dispersed quinone compounds for 4–10 hours, yielding a dye reducing solution. The target cotton fabric is then placed in the dye reducing solution for dyeing treatment to obtain the dyed fabric.

[0008] As a preferred technical solution:

[0009] The green and efficient biological reduction method for quinone-based natural compounds used in textile dyeing, as described above, comprises, by weight parts: 1-4 parts peptone; 1-2 parts yeast extract; 0.5-2 parts sodium chloride; and 100 parts sterile water.

[0010] The green and efficient bioreduction method for quinone-based natural compounds used in textile dyeing, as described above, includes the process of highly dispersing the quinone-based natural compounds by mixing the quinone-based natural compounds with water to form a dye mixed solution, and then fully dispersing the mixed solution at 0-4°C under the action of an ultrasonic cell disruptor with a power of 400-750W for later use.

[0011] The green and efficient biological reduction method for quinone-based natural compounds used in textile dyeing, as described above, uses anthraquinone-2,6-disulfonic acid, flavin mononucleotide, coenzyme Q, and other substances with redox activity, added in amounts of 0.2–1.2 mmol / L.

[0012] The green and efficient biological reduction method for quinone-based natural compounds used in textile dyeing, as described above, involves inoculating a sterilized culture medium with 5-20% Shewanella putrefactive bacteria seed solution and Saccharomyces cerevisiae, and culturing it at 180-240 rpm and 28-35°C until the logarithmic growth phase.

[0013] The green and efficient biological reduction method for quinone-based natural compounds used in textile dyeing, as described above, involves an electrode microbial mixture composed of 20%–40% plateau-stage Saccharomyces cerevisiae and 60%–80% plateau-stage Shewanella putrefactiveis.

[0014] The aforementioned green and efficient bio-reduction method for textile dyeing using quinone-based natural compounds includes the following steps: The target fabric is immersed in the natural quinone compound reducing solution for dyeing. The fabric is then added to the dye reducing solution for immersion in the solution at a bath ratio of 1:50. After every 4-10 hours of immersion, the fabric is removed and oxidized in air for 5-20 minutes. Nutrient additives are then added to the dye reducing solution. These additives include 0.01-0.08 parts beef powder and 0.01-0.05 parts potassium dihydrogen phosphate, all by weight. The immersion and oxidation process is repeated 3-5 times, followed by soaping to obtain the dyed fabric.

[0015] The principle of this invention is as follows:

[0016] From an ecological perspective, replacing traditional chemical reduction processes with microbial processes, including those involving cells and enzymes, may be a very attractive option. Biocatalytic reduction is an important method for maintaining sustainable development and replacing traditional highly polluting chemical methods and organic chemical synthesis. Based on the pollution problems caused by the current use of mordants to dye textiles with quinone natural compounds, a whole-cell reduction system was constructed using highly efficient reducing bacteria *Shewanella putrefactiveis* (ATCC49138) and *Saccharomyces cerevisiae* (ATCC9763). Furthermore, by adding redox factors to accelerate the transfer of quinone natural compounds to the electrode microbiota, the bioreduction dyeing effect of this invention was further enhanced.

[0017] The above technical solution has the following beneficial effects:

[0018] (1) Compared with the chemical reagents commonly used in printing and dyeing production, the whole-cell catalytic bioreduction production method is greener and more environmentally friendly, and has the advantage of sustainable production.

[0019] (2) Compared with traditional fermentation and dyeing techniques, the whole-cell biocatalytic reduction system constructed in this invention achieves controllable strains, controllable process, and controllable efficiency, forming a processing technology that can be used for industrial production.

[0020] (3) By introducing redox factors, the electron transfer rate of the electrode bacteria is accelerated, thereby improving the dyeing effect on textiles.

[0021] (4) The biological dyeing method constructed in this invention reduces the use of high-risk chemicals and is carried out under normal temperature conditions. It is a green printing and dyeing processing technology with high technological content, low resource consumption, low environmental pollution and high efficiency. Detailed Implementation

[0022] Example 1

[0023] A green and efficient bioreduction method for quinone-based natural compounds used in textile dyeing includes the following steps:

[0024] One part peptone, two parts yeast extract, and 0.5 parts sodium chloride were mixed evenly in 100 parts sterile water and sterilized at 121°C and 0.1 MPa for 15 min to obtain the sterilized culture medium. *Shewanella putrefactiveis* (ATCC 49138) and *Saccharomyces cerevisiae* (ATCC 9763) were inoculated at a 5% inoculation rate into the cooled sterilized culture medium and cultured with shaking at 220 rpm and 30°C for 12 h to obtain two microbial cultures. An electrode microbial consortium was prepared by mixing 20% ​​*Saccharomyces cerevisiae* and 80% *Shewanella putrefactiveis*. 0.04123 parts of anthraquinone-2,6-disulfonic acid were added to 100 parts of the electrode microbial consortium to achieve a concentration of 1 mmol / L. One part alizarin was added to 10 parts deionized water and ultrasonically dispersed at 4°C using a 500W ultrasonic cell disruptor. This dispersion was then added to an electrode bacterial culture mixture containing anthraquinone-2,6-disulfonic acid. The mixture was stirred at 100 rpm for 5 minutes to ensure homogeneity and then allowed to stand at 25°C for 8 hours for reduction. The soaked cotton fabric was then added to the reduced dye solution at a liquor ratio of 1:50 for dyeing. Every 4 hours, the fabric was removed from the dye bath and oxidized by ventilation for 5 minutes. 0.01 parts beef powder and 0.05 parts potassium dihydrogen phosphate were added to the dye reducing solution. This dyeing and oxidation process was repeated three times on the target fabric, followed by soaping to obtain the dyed fabric.

[0025] Example 2

[0026] A green and efficient bioreduction method for quinone-based natural compounds used in textile dyeing includes the following steps:

[0027] Two parts peptone, three parts yeast extract, and 0.5 parts sodium chloride were mixed evenly in 100 parts sterile water and sterilized at 121°C and 0.1 MPa for 15 min to obtain the sterilized culture medium. *Shewanella putrefactiveis* (ATCC 49138) and *Saccharomyces cerevisiae* (ATCC 9763) were inoculated at a 5% inoculation rate into the cooled sterilized culture medium and cultured with shaking at 220 rpm and 30°C for 12 h to obtain two microbial cultures. An electrode microbial consortium was prepared by mixing 20% ​​*Saccharomyces cerevisiae* and 80% *Shewanella putrefactiveis*. 0.04123 parts of anthraquinone-2,6-disulfonic acid were added to 100 parts of the electrode microbial consortium to achieve a concentration of 1 mmol / L. Three parts of juglone were added to 10 parts of deionized water and ultrasonically dispersed in a 600W ultrasonic cell disruptor at 4°C. This dispersion was then added to an electrode bacterial culture mixture containing anthraquinone-2,6-disulfonic acid. The mixture was stirred at 120 rpm for 10 minutes to ensure homogeneity and then allowed to stand at 25°C for 12 hours for reduction. The soaked cotton fabric was then added to the reduced dye solution at a liquor ratio of 1:50 for dyeing. Every 8 hours, the fabric was removed from the dye bath and oxidized by ventilation for 15 minutes. 0.01 parts of beef powder and 0.05 parts of potassium dihydrogen phosphate were added to the dye reducing solution. This dyeing and oxidation process was repeated three times on the target fabric, followed by soaping to obtain the dyed fabric.

[0028] Example 3

[0029] A green and efficient bioreduction method for quinone-based natural compounds used in textile dyeing includes the following steps:

[0030] Three parts peptone, one part yeast extract, and one part sodium chloride were mixed evenly in 100 parts sterile water and sterilized at 121°C and 0.1 MPa for 15 min to obtain the sterilized culture medium. *Shewanella putrefactiveis* (ATCC 49138) and *Saccharomyces cerevisiae* (ATCC 9763) were inoculated at a 5% inoculation rate into the cooled sterilized culture medium and cultured with shaking at 220 rpm and 30°C for 12 h to obtain two microbial cultures. An electrode microbial consortium was prepared by mixing 30% *Saccharomyces cerevisiae* and 70% *Shewanella putrefactiveis*. 0.0478 parts of flavin mononucleotide were added to 100 parts of the electrode microbial consortium to achieve a concentration of 1 mmol / L. Three parts of juglone were added to 10 parts of deionized water and ultrasonically dispersed at 4°C using a 700W ultrasonic cell disruptor. This dispersion was then added to an electrode microbial mixture containing flavin mononucleotides and stirred at 150 rpm for 10 minutes to ensure homogeneity. The mixture was then allowed to stand at 30°C for 12 hours for reduction. The soaked cotton fabric was then added to the reduced dye solution at a liquor ratio of 1:50 for dyeing. Every 8 hours, the fabric was removed from the dye bath and oxidized by ventilation for 15 minutes. 0.01 parts of beef powder and 0.05 parts of potassium dihydrogen phosphate were added to the dye reducing solution. This dyeing and oxidation process was repeated three times on the target fabric. Finally, the fabric was soaped to obtain the dyed fabric.

[0031] The comparison results using the method of the present invention are shown in Table 1.

[0032] Table 1

[0033]

[0034] The above are merely specific embodiments of the present invention, but the technical features of the present invention are not limited thereto. Any simple changes, equivalent substitutions, or modifications made based on the present invention to solve essentially the same technical problems and achieve essentially the same technical effects are all covered within the protection scope of the present invention.

Claims

1. A green and efficient bioreduction method for quinone-based natural compounds used in textile dyeing, characterized in that... Includes the following steps: (1) Add 1-4 parts of peptone, 1-2 parts of yeast extract, and 0.5-2 parts of sodium chloride to 100 parts of deionized water to dissolve and prepare a culture medium. Sterilize at 121℃ and 0.1MPa for 15 min, then transfer 5-20% of logarithmic-phase Shewanella putrefactive bacteria (ATCC49138) and Saccharomyces cerevisiae (ATCC 9763) to the medium and incubate at 180-240r and 28-35℃ for 10-12 h. Mix 20%-40% of plateau-phase Saccharomyces cerevisiae and 60%-80% of plateau-phase Shewanella putrefactive bacteria to form an electrode microbial mixture for later use. (2) Add the redox mediator composed of anthraquinone-2,6-disulfonic acid, flavin mononucleotide, and coenzyme Q to the electrode bacterial culture mixture in step (1) and make its concentration in the mixture 0.2-1.2 mmol / L for later use; (3) Add 1 to 3 parts of alizarin or jujube quinone and other quinone natural compounds to 10 parts of deionized water, and perform ultrasonic dispersion treatment in an ultrasonic cell disruptor with a power of 400 to 750w at 0 to 4℃ to form a quinone natural compound dispersion system for later use. (4) Add the quinone natural compound dispersion from step (3) to the electrode bacterial mixture containing redox factors prepared in step (2), stir at 100-150 r / min for 5-10 min, mix thoroughly, and allow to stand at 25-30℃ for 8-12 h to form a reducing solution for later use. All raw materials are in parts by weight. (5) Add the soaked cotton textile fabric to the dye reducing solution prepared in step (4) and perform immersion dyeing at a bath ratio of 1:

50. Take it out of the dye bath every 4 to 10 hours for ventilation and oxidation for 5 to 20 minutes. Add 0.01 to 0.08 parts of beef powder and 0.01 to 0.05 parts of potassium dihydrogen phosphate to the dye reducing solution. Repeat the immersion and oxidation process on the target fabric 3 to 5 times, and then perform soap washing treatment to obtain the dyed fabric.

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