Sample analyzer and method for processing reagent bubbles

Abstract

This application provides a sample analyzer and a method for processing reagent bubbles. The sample analyzer includes: a reagent device for placing a reagent container, the reagent container being used to store reagents; a dispensing device for drawing reagents from the reagent container and discharging them into a reaction container to obtain a reaction solution; a detection device for detecting the reaction solution; a bubble recognition mechanism for performing bubble recognition operations on the reagent container and outputting corresponding bubble recognition information; and a control device for: controlling the bubble recognition mechanism to perform bubble recognition operations on the reagent container and receiving the bubble recognition information output by the bubble recognition mechanism; controlling the sample analyzer to perform bubble pretreatment on the reagent container based on the bubble recognition information; and controlling the detection device to detect the target reaction solution to obtain corresponding detection data, wherein the target reaction solution is formed by mixing the reagents and samples in the pretreated reagent container.

Description

Technical Field

[0001] This application relates to the field of medical device technology, and in particular to a sample analyzer and a method for processing reagent bubbles. Background Technology

[0002] Sample analyzers are instruments used in the medical device field to detect and analyze biological chemical substances. Sample analyzers typically require the aspiration of reagents from containers. Air bubbles can easily form during the processes of reagent filling, transporting, and loading the reagent containers onto the instrument. These air bubbles can cause the aspiration of reagents into the analyzer, resulting in inaccurate reagent volumes and ultimately affecting the accuracy of the sample test results. Summary of the Invention

[0003] The main objective of this application is to provide a sample analyzer and a method for processing reagent bubbles. Before using the reagent in the reagent container to test the sample, the method aims to obtain bubble identification information in the reagent container and perform corresponding bubble pretreatment to reduce the possibility of empty or insufficient aspiration during reagent aspiration, thereby improving the accuracy of reagent aspiration and ultimately effectively improving the accuracy of the test results.

[0004] In a first aspect, embodiments of this application provide a sample analyzer, comprising: A reagent device is provided with at least one placement position for placing a reagent container for storing reagents; The dispensing device is at least used to draw up the reagent from the reagent container and discharge it into the reaction container so that the sample and reagent are mixed in the reaction container to form a reaction solution. A detection device is used to detect the reaction solution in order to obtain corresponding detection data; A bubble recognition mechanism is used to perform bubble recognition operations on the reagent container and output corresponding bubble recognition information; Control device, at least for: Before the dispensing device draws the reagent from the reagent container, the bubble recognition mechanism is controlled to perform a bubble recognition operation on the reagent container, and the bubble recognition information output by the bubble recognition mechanism during the bubble recognition operation is received. Based on the bubble recognition information, the sample analyzer is controlled to perform bubble pretreatment on the reagent container. The bubble pretreatment includes at least one of bubble removal operation and bubble avoidance operation. The detection device is controlled to detect the target reaction solution and obtain corresponding detection data, wherein the target reaction solution is formed by mixing the reagent and sample in the reagent container after the bubble pretreatment.

[0005] Secondly, embodiments of this application provide a method for processing reagent bubbles, applied to a sample analyzer, the method comprising: Before the dispensing device of the sample analyzer draws the reagent from the reagent container, the bubble recognition mechanism of the sample analyzer is controlled to perform a bubble recognition operation on the reagent container, and the bubble recognition information output by the bubble recognition mechanism is received. Based on the bubble recognition information, the sample analyzer is controlled to perform bubble pretreatment on the reagent container. The bubble pretreatment includes at least one of bubble removal operation and bubble avoidance operation. The detection device of the sample analyzer is controlled to detect the target reaction solution and obtain corresponding detection data. The target reaction solution is formed by mixing the reagent in the reagent container after the bubble pretreatment with the sample to be tested.

[0006] In the above embodiments, before the dispensing device draws the reagent from the reagent container, a bubble recognition mechanism performs a bubble recognition operation on the reagent container to obtain the bubble recognition information corresponding to the reagent container. Then, based on the bubble recognition information, bubbles can be eliminated or bubble avoidance can be performed to reduce the possibility of empty or insufficient aspiration during the reagent aspiration process, thereby improving the accuracy of reagent aspiration and ultimately effectively improving the accuracy of the test results.

[0007] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit the disclosure of the embodiments of this application. Attached Figure Description

[0008] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0009] Figure 1 This is a block diagram of the sample analyzer in one embodiment; Figure 2 This is a schematic diagram of the structural layout of a sample analyzer in one embodiment; Figure 3 This is a schematic diagram of the sample dispensing device of a sample analyzer in one embodiment; Figure 4 This is a schematic diagram illustrating a scenario in which a sample analyzer identifies air bubbles in a reagent container based on electrical properties. Figure 5This is a schematic diagram illustrating the change in the electrical properties of a pipette during the process of a sample analyzer identifying air bubbles in a reagent container using its electrical properties, as described in one embodiment. Figure 6 This is a three-dimensional structural diagram of the reagent container used by the sample analyzer for sample testing in one embodiment. Figure 7 This is a partial cross-sectional view of the reagent container in one embodiment. Figure 8 This is a schematic diagram of the reagent container in an open state in one embodiment; Figure 9 This is a schematic diagram illustrating a scenario in which reagent is drawn from a reagent container that has completed the bubble removal operation, according to one embodiment. Figure 10 This is a schematic diagram illustrating a scenario in which reagents are drawn from a reagent container using a bubble-avoiding operation in one embodiment. Figures 11 to 13 These are schematic diagrams illustrating scenarios with varying bubble layer heights within the reagent container across multiple implementation methods; Figures 14 to 16 These are schematic diagrams illustrating different bubble distributions within the reagent container in various implementation methods; Figures 17 to 20 These are schematic diagrams illustrating different implementation methods of a sample analyzer removing air bubbles from a reagent container. Figure 21 This is a schematic diagram of reagent image data acquired by a sample analyzer when acquiring images of a reagent container in one embodiment; Figure 22 This is a schematic diagram of reagent image data obtained by a sample analyzer acquiring images of a reagent container in one embodiment; Figure 23 This is a schematic diagram of reagent image data acquired by the sample analyzer when it captures images of the reagent container in another embodiment. Figure 24 This is a flowchart illustrating the steps of a reagent bubble treatment method provided in this application, as one embodiment. Detailed Implementation

[0010] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0011] In the description of this application, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0012] The flowchart shown in the attached diagram is for illustrative purposes only and does not necessarily include all content and operations / steps, nor does it necessarily have to be performed in the order described. For example, some operations / steps can be broken down, combined, or partially merged, so the actual execution order may change depending on the actual situation.

[0013] The following detailed description of some embodiments of this application is provided in conjunction with the accompanying drawings. Unless otherwise specified, the following embodiments and features can be combined with each other.

[0014] Sample analyzers are instruments used in the medical device field to detect and analyze biological chemical substances. Sample analyzers typically require the aspiration of reagents from containers. Air bubbles can easily be generated during the processes of reagent filling, transporting, and loading the reagent containers onto the instrument. These air bubbles can cause the aspiration process to result in either empty aspiration or insufficient aspiration, leading to reduced accuracy of the test results.

[0015] This application attempts to address one or more of the above-mentioned pain points by performing a bubble recognition operation on the reagent container before testing the sample using the reagent in the container. This allows for the acquisition of bubble recognition information for the reagent container, which can then be used to eliminate or avoid bubbles. This reduces the possibility of empty or insufficient aspiration during reagent dispensing, thereby improving the accuracy of reagent aspiration and ultimately enhancing the accuracy of test results.

[0016] Please see Figures 1 to 2 This application provides a sample analyzer 100 for analyzing a sample to be tested to obtain corresponding analytical results. The sample analyzer 100 includes, but is not limited to, at least one of the following: a biochemical analyzer, an immunoassay analyzer, a coagulation analyzer, a urine analyzer, and a molecular diagnostic analyzer.

[0017] like Figure 1 As shown, the sample analyzer 100 includes a dispensing device 10, a sample device 20, a reagent device 30, a reaction device 40, a detection device 50, and a control device 70.

[0018] The sample device 20 is used to provide a sample, and the reagent device 30 is used to provide reagents that react with the sample. For example, the reagent device 30 is provided with at least one placement position 301b for placing a reagent container 90. The reagent container 90 is used to store reagents, including but not limited to chromogenic reagents, diluents, substrate solutions, enzyme-labeled reagents, magnetic bead reagents, etc.

[0019] Dispensing device 10 is used to perform dispensing operations to dispense target liquid into a target container, wherein the target liquid includes at least one of a sample, a reagent, and a reaction solution, and the reaction solution is formed by at least a mixture of the sample and the reagent.

[0020] Optionally, the dispensing operation includes a reagent dispensing operation and a sample dispensing operation. The reagent dispensing operation involves drawing reagents from reagent container 90 and dispensing them into a target container (e.g., a reaction container) to mix the sample and reagents within the reaction container, forming a reaction solution. The sample dispensing operation involves drawing samples from sample container and dispensing them into a target container (e.g., a reaction container) to mix the sample and reagents within the reaction container, forming a reaction solution. For example, the dispensing device 10 can be used to draw samples from a sample container (e.g., a sample tube) supplied by the sample device 20, transfer the samples to a target container (e.g., a reaction container), and dispensing the drawn samples into the target container, thereby completing the sample dispensing. As another example, the dispensing device 10 can be used to draw reagents from a reagent container 90 (e.g., a reagent bottle) supplied by the reagent device 30, transfer the reagents to a target container (e.g., a reaction container), and dispensing the drawn reagents into the target container, thereby completing the reagent dispensing.

[0021] The reaction apparatus 40 is provided with at least one reaction position for placing a reaction container. This reaction container is used to receive samples and reagents and to provide a mixing space for the samples and reagents. The reaction container includes, but is not limited to, a reaction cup. For example, during sample and reagent mixing, the dispensing device 10 aspirates the sample supplied by the sample device 20 and the reagent supplied by the reagent device 30, and dispenses the aspirated sample and reagent into the reaction container placed in the preset operating position, so that the sample and reagent mix within the reaction container to form a reaction solution. Optionally, the reaction apparatus 40 is also used to incubate the reaction solution formed by the mixture of samples and reagents.

[0022] The detection device 50 is used to detect and analyze the reaction solution formed by mixing the sample and reagents to obtain detection data. For example, the detection device 50 is used to detect the reaction solution in the reaction vessel to obtain the sample detection data.

[0023] Optionally, the detection device 50 includes a photometric mechanism for detecting the luminescence intensity of the reaction liquid to obtain the luminescence intensity of the reaction liquid. Then, using a calibration curve and the luminescence intensity, the concentration of the analyte in the sample can be calculated. It is understood that the detection data acquired by the detection device 50 can be either the luminescence intensity of the reaction liquid or the concentration of the analyte in the reaction liquid. That is, calculating the concentration of the analyte in the sample using a calibration curve and the luminescence intensity can be performed by either the detection device 50 or the control device 70; no limitation is made here. Optionally, the detection device 50 is separately disposed around the reaction device 40.

[0024] In another embodiment, the detection device 50 includes an electrical detection mechanism (e.g., an impedance detection mechanism) or a detection mechanism based on other principles (e.g., an imaging detection mechanism).

[0025] Skilled technicians should understand that Figure 1 This is merely an example of a sample analyzer 100 and does not constitute a limitation on the sample analyzer 100. The sample analyzer 100 may include components such as... Figure 1 The sample analyzer 100 may include more or fewer components, or combinations of certain components, or different components. It may also include input / output devices, network access devices, etc.

[0026] In some embodiments, the sample analyzer 100 further includes a scheduling device 60, which is used to perform scheduling operations to achieve the scheduling of the target object. Specifically, the scheduling device 60 is used to move the target object in two-dimensional or three-dimensional space to achieve the scheduling of the target object. The target object includes, but is not limited to, at least one of a sample container, a reagent container 90, and a reaction container. Optionally, the scheduling device 60 includes at least a first scheduling component and / or a second scheduling component. The first scheduling component may be a mechanical gripper used to schedule by gripping the target object. The second scheduling component is provided with a scheduling position. After the target object is placed in the scheduling position, it can move the target object in two-dimensional or three-dimensional space to achieve the scheduling of the target object.

[0027] It is understood that there can be one or more scheduling devices 60. The same target object can be scheduled in different areas through the same scheduling device 60, or the same target object can be scheduled in different areas through different scheduling devices 60. There are no restrictions here.

[0028] For example, the scheduling device 60 can serve as a reagent scheduling device to at least perform the scheduling of the reagent container 90. For instance, the scheduling device 60 can grasp the reagent container 90 and move it in a two-dimensional or three-dimensional space to achieve unidirectional or bidirectional reciprocating scheduling of the reagent container 90 between at least two different locations.

[0029] Alternatively, the scheduling device 60 can serve as a sample scheduling device to at least perform the scheduling of sample containers. For example, the scheduling device 60 can grasp a sample container and move it in two-dimensional or three-dimensional space to achieve unidirectional or bidirectional reciprocating scheduling of the sample container between at least two different locations.

[0030] In some embodiments, the reaction apparatus 40 has a support portion 401, and the support portion 401 is provided with at least one reaction position for placing a reaction container (such as a reaction cup 4011). The reaction container is used to receive samples and reagents and to provide a reaction site for the samples and reagents to mix and form a reaction solution. For example, the reaction container receives a sample obtained by the dispensing device 10 from the sample device 20 and a reagent obtained by the reagent device 30, so that the samples and reagents are mixed in the reaction container to form a reaction solution.

[0031] Optionally, the support portion 401 of the reaction apparatus 40 can be a reaction disk, such as... Figure 2 As shown, it is arranged in a disc-shaped assembly and has one or more reaction positions for placing reaction containers. The reaction disc can incubate the reaction liquid in the reaction container and can rotate to drive the reaction container placed in the reaction position to rotate, so as to realize the scheduling of the reaction container in the reaction disc in a preset area. For example, the reaction container located in the reaction position can be scheduled to the position for reagent addition so that the dispensing device 10 can discharge the aspirated reagent into the reaction container located at the reagent addition position.

[0032] It is understood that the reaction position used to support the reaction vessel can be located not only on the reaction plate of the reaction apparatus 40, but also independently of the reaction plate of the reaction apparatus 40. Setting the reaction position independently of the reaction plate means that the setting of the reaction position will not interfere with the rotation of the reaction plate itself.

[0033] like Figure 2 As shown, in some embodiments, the sample device 20 may include a sample delivery module (SDM) and a front-end track; the sample is placed in the sample tube, and the sample rack carrying one or more sample tubes is dispatched to the corresponding injection position via the front-end track, and the sample delivery module transfers the sample rack located at the injection position to a preset position (such as the aspiration position).

[0034] In other examples, the sample device 20 may also be a sample tray, which includes multiple sample positions for placing sample tubes, and the sample tray can be rotated to move the sample to the appropriate position, for example, to the aspiration position of the dispensing device 10 for aspirating the sample in the sample tube located at the aspiration position and discharging it into the reaction vessel to be added.

[0035] In some embodiments, the reagent component 30 can be a disc-shaped structure or a linear structure. For example, the reagent component 30 includes a reagent carrying mechanism 301, which is disc-shaped (also called a reagent tray). The reagent carrying mechanism 301 is provided with a plurality of reagent positions 301b, and the reagent carrying mechanism 301 is rotatable, thereby rotating the reagent container 90 carried by the reagent position 301b. By rotating the reagent carrying mechanism 301, the reagent carried by the reagent container 90 can be transferred to the reagent aspiration position so that the dispensing device 10 can aspirate the reagent from the reagent container 90 located at the reagent aspiration position. The reagent carrying mechanism 301 may have an openable and closable reagent tray cover, which allows the user to put reagent into or remove reagent from the reagent carrying mechanism 301 when the reagent tray cover is opened.

[0036] Optionally, the reagent carrying mechanism 301 may include at least one rotatable reagent track 301a, the reagent track 301a being provided with a plurality of placement positions 301b for carrying reagents, and the reagent track 301a rotating to drive the reagent container 90 on its placement position 301b to move.

[0037] Optionally, the reagent carrying mechanism 301 includes multiple reagent tracks 301a, each capable of independent rotation. The reagent tracks 301a can rotate and drive the reagent container 90 they carry to move, thereby rotating the reagent container 90 to the reagent aspiration position for the dispensing device 10 to aspirate the reagent.

[0038] Please see Figure 3 In some embodiments, the dispensing device 10 includes a sample dispensing component 10a, which performs a sample dispensing operation. Specifically, the sample dispensing component 10a aspirates the sample supplied by the sample device 20 and transfers the sample to a preset location. For example, the sample device 20 carries a sample tube containing the sample to be tested, and the sample dispensing component 10a aspirates the sample to be tested from the sample tube carried by the sample device 20 and discharges the sample into the reaction container to be added.

[0039] Optionally, the sample dispensing component 10a includes a dispensing needle 101 and a first needle moving mechanism 102, which supports the dispensing needle 101 and drives it to move. For example, the dispensing needle 101 can move in two or three dimensions in space via the two-dimensional or three-dimensional first needle moving mechanism 102, thereby allowing the dispensing needle 101 to move and aspirate the sample carried by the sample device 20.

[0040] Optionally, the sample dispensing component 10a further includes a first power mechanism 103, which provides power for the dispensing needle 101 to perform sample aspiration and / or dispensing, thereby completing the sample aspiration and / or dispensing. For example, taking the dispensing needle 101 performing sample aspiration as an example, the dispensing needle 101 moves under the drive of the first needle moving mechanism 102 to the sample tube containing the sample to be tested on the sample device 20, and aspirates the sample to be tested under the drive of the first power mechanism 103, and delivers the sample to be tested to the reaction container located in the reaction position of the reaction device 40, so that the sample to be tested aspirated by the sample dispensing component 10a is mixed with the reagent provided by the reagent device 30 in the reaction container.

[0041] like Figure 3 As shown, in some embodiments, the first needle moving mechanism 102 includes a support frame 1021, which is fixed to a support rod 1022. The support rod 1022 is vertically movable and rotatable, and the support frame 1021 moves vertically and rotates horizontally under the drive of the support rod 1022. The sample dispensing needle 101 is mounted on the support frame 1021 and can reach the target position under the drive of the support frame 1021. Exemplarily, the first needle moving mechanism 102 also includes a driver 1023 for driving the support rod 1022 to move, such as a stepper motor, but is not limited to this. Optionally, the sample dispensing needle 101 is detachably connected to the first needle moving mechanism 102 or fixedly connected.

[0042] Optionally, the first power mechanism 103 includes a tubing 1031 and a power assembly 1033. The tubing 1031 is used to transport a fluid medium. One end of the tubing 1031 is connected to the sampling needle 101, and the other end is connected to the power assembly 1033, so that the flow direction of the fluid medium in the tubing 1031 can be changed under the action of the power assembly 1033, so that the sampling needle 101 can transfer samples and / or reagents. The power assembly 1033 includes, but is not limited to, a syringe, a pump, and a valve body disposed on the tubing 1031.

[0043] like Figure 2 As shown, in some embodiments, the dispensing device 10 further includes a reagent dispensing component 10b, which is used to perform a reagent dispensing operation. That is, the reagent dispensing component 10b is used to draw the reagent supplied by the reagent device 30 and transfer the reagent to a preset position. For example, the reagent device 30 carries a reagent container 90 containing reagents, and the reagent dispensing component 10b draws the reagent from the reagent container 90 carried by the reagent device 30 and discharges the reagent into the reaction vessel to be added with the reagent.

[0044] In some embodiments, the reagent dispensing component 10b may include a reagent needle, a second needle moving mechanism, and a second power mechanism. The reagent needle moves in two or three dimensions in space via the two-dimensional or three-dimensional second needle moving mechanism, thereby allowing the reagent needle to move and cooperate with the second power mechanism to draw the reagent carried by the reagent carrying mechanism 301, and to move to the reaction container to which the reagent is to be added, and to discharge the reagent into the reaction container.

[0045] In some embodiments, the second needle moving mechanism and the first needle moving mechanism 102 have the same structure, and / or the second power mechanism and the first power mechanism 103 have the same structure, which will not be described in detail here.

[0046] In some embodiments, the reagent dispensing component 10b adds reagents not via a reagent needle, but through a dedicated tubing to add the reagent from the reagent tube into the reaction vessel. In these embodiments, only the sample dispensing needle 101 is used, without a reagent needle.

[0047] It is understandable that, depending on the type of bodily fluid being tested and the specific test being performed, different methods of adding samples and reagents may be used. For example, both samples and reagents may be added using the sampling needle 101, or samples may be added using the sampling needle 101 and reagents using the reagent needle, or only samples may be added using the sampling needle 101 and reagents using other methods. That is, the sample dispensing component 10a of the dispensing device 10 is used for both sample transfer and reagent transfer; or the sample dispensing component 10a is used for sample transfer and the reagent dispensing component 10b is used for reagent transfer; or the sample dispensing component 10a is used for sample transfer, and the reagent is added to the reaction vessel via a dedicated tubing connected to the reagent container 90. Therefore, the sampling needle 101 and / or the reagent needle are also referred to as pipettes, meaning that a pipette includes at least one of the sampling needle 101 and the reagent needle.

[0048] As described above, the dispensing device 10 includes a pipette (e.g., a sample dispensing needle 101, a reagent needle), a needle movement mechanism (e.g., a first needle movement mechanism, a second needle movement mechanism), and a power mechanism (e.g., a first power mechanism, a second power mechanism). The pipette is used to transfer samples and / or reagents. The needle movement mechanism is used to drive the pipette to move. The power mechanism is used to provide power for the pipette to aspirate and dispense samples and / or reagents.

[0049] In some embodiments, the sample analyzer 100 further includes a bubble recognition mechanism 81, used to perform bubble recognition operations on the reagent container 90 and output corresponding bubble recognition information. This allows the control device 70 to analyze the bubble recognition information (also known as bubble detection) using a preset algorithm to obtain the corresponding bubble recognition result. Based on the bubble recognition result, it can determine whether there is an abnormal bubble condition within the reagent container 90. If an abnormal bubble condition exists in the reagent container 90, bubble pretreatment is performed on the reagent container 90. Bubble pretreatment includes at least one of the following: a bubble layer exists within the reagent container 90 and the height of the bubble layer exceeds a height threshold; the reagent container 90 contains bubbles and the liquid surface area covered by the bubbles exceeds an area threshold; or bubbles exist at the liquid surface position corresponding to the liquid aspiration operation performed by the dispensing device 10 within the reagent container 90.

[0050] In some embodiments, the bubble recognition mechanism 81 includes an image acquisition mechanism 81a for acquiring images to obtain corresponding image data. For example, the image acquisition mechanism 81a is used to acquire images of the reagents stored in the reagent container 90 to obtain corresponding reagent image data, and transmits the reagent image data as bubble recognition information to the control device 70. The control device 70 then analyzes the bubble recognition information (also known as bubble detection) using a preset algorithm to obtain the corresponding bubble recognition result, thereby determining whether there is an abnormality of bubbles in the reagent container 90 based on the bubble recognition result.

[0051] Please see Figure 3 and Figure 5 In some embodiments, the electrical characteristics of the pipette may change when it comes into contact with a target object, which includes at least air bubbles. The bubble recognition mechanism 81 includes a pipette and a needle signal processing unit. The needle signal processing unit is connected to the pipette and is used to sample the changes in the electrical signal of the pipette to output corresponding bubble recognition information. For example, when the pipette comes into contact with a liquid surface, air bubbles, or other target objects, its capacitance characteristics change. The needle signal processing unit collects the capacitance characteristics of the pipette and converts them into corresponding electrical signals (such as current signals, voltage signals, or frequency signals), thereby outputting corresponding bubble recognition information.

[0052] Based on the different changes in capacitance characteristics when a pipette contacts the liquid surface and when it contacts an air bubble, by detecting the change in capacitance characteristics of the pipette as it descends towards the liquid surface in the reagent container 90, information such as the presence of air bubbles in the reagent container 90, the thickness of the air bubble layer in the reagent container 90, and the distribution of air bubbles in the reagent container 90 can be detected.

[0053] like Figure 4 and Figure 5As shown, the capacitance characteristic of the pipette is detected starting at time T0. During the time interval from T0 to T1, the pipette does not contact the air bubble. At time T1, it contacts the air bubble, and the capacitance characteristic of the pipette undergoes a first state change. During the time interval from T1 to T2, the pipette continuously contacts the air bubble. At time T2, it begins to contact the liquid surface, and the capacitance characteristic of the pipette undergoes a second state change. For example, through... Figure 5 Analysis shows that there are air bubbles above the liquid surface area contacted by the pipette, and the height of the bubble layer is H = (T2 - T1)·S, where S is the descent speed of the pipette during the time period from T1 to T2.

[0054] In some embodiments, the sample analyzer 100 further includes a reagent loading device 80, which has at least one loading position 801 for carrying a reagent container 90. After the reagent container 90 is placed in the loading position 801, a bubble recognition mechanism 81 can be used to perform bubble recognition on the reagent container 90 to obtain bubble recognition information. The control device 70 can analyze the bubble recognition information through a preset algorithm to obtain the corresponding bubble recognition result. Based on the bubble recognition result, it can determine whether there is an abnormal bubble in the reagent container 90 and output the corresponding abnormality handling command to control the sample analyzer 100 to perform the corresponding bubble abnormality handling operation.

[0055] In some embodiments, the sample analyzer 100 further includes a bubble removal device 82 for performing a bubble removal operation to remove at least some of the bubbles within the reagent container 90. For example, if there is an abnormality in the presence of bubbles within the reagent container 90, the control device 90 can control the bubble removal device 82 to perform a bubble removal operation on the reagent container 90 to reduce or even eliminate the impact of the abnormality in the reagent container 90 on subsequent sample analysis.

[0056] Optionally, the bubble removal device 82 includes an ultrasonic component 821. In the event of an abnormality in the reagent container 90, the control device 70 can control the ultrasonic component to output ultrasonic waves to the bubble layer in the reagent container 90 to remove at least some of the bubbles in the reagent container 90.

[0057] Optionally, the bubble removal device 82 includes a pressure component 822. In the event of an abnormality in the reagent container 90, the control device 70 can control the pressure component to change the air pressure inside the reagent container 90 in order to remove at least some of the bubbles inside the reagent container 90.

[0058] Optionally, the bubble removal device 82 includes a dispensing device 10. In the event of an abnormality in the reagent container 90, the control device 70 can control the dispensing device 10 to perform a bubble removal operation on the reagent container 90 to remove at least some of the bubbles in the reagent container 90. For example, the dispensing device 10 can be controlled to draw in the bubbles in the reagent container 90 to cause the bubbles in the reagent container 90 to break, thereby achieving the removal of bubbles in the reagent container 90.

[0059] Please see Figures 6 to 8 In some embodiments, the reagent container 90 includes a container body 91 and a cap 92, wherein the container body 91 is formed with a storage cavity 912 having an opening 911 for storing reagents, and the cap 92 covers the opening 911 of the container body 91 to seal the opening 911.

[0060] Exemplarily, the cover 92 includes a cover portion 921, a connecting portion 922, and a sealing portion 923. The cover portion 921 is connected to the connecting portion 922 and is connected to the container body 91 via the connecting portion 922. The sealing portion 923 is connected to the cover portion 921 and is used to seal the opening 911 of the container body 91. For example, the connecting portion 922 and the container body 91 are detachably connected by means of threaded connection, structural engagement, interference fit, etc., and the connecting portion 922 forms a communicating hole 9221 communicating with the opening 911. The sealing portion 923 seals the opening 911 of the container body 91 by sealing the communicating hole 9221. Alternatively, the connecting portion 922 and the container body 91 are detachably connected by means of threaded connection, structural engagement, interference fit, etc., and the sealing portion 923 is adapted to the opening 911 of the container body 91 to seal the opening 911 of the container body 91.

[0061] In some embodiments, the sample analyzer 100 further includes a pretreatment device 83, which is used to release the sealing fit between the container body 91 and the cap 92 so that the dispensing device 10 can draw reagents from the reagent container 90. For example, the pretreatment device 83 includes a first pretreatment component and a second pretreatment component. The first pretreatment component is used to limit the container body 91, and the second pretreatment component is used to apply force to the cap 92 so that the sealing part 923 and the connecting part 922 or the opening 911 are disengaged from the sealing fit, thereby causing the body 91 and the cap 92 to disengage from the sealing fit.

[0062] like Figure 6 As shown, exemplarily, to prevent contamination of the reagent in the reagent container 90 during transportation, the container body 91 and the cap 92 are typically in a sealed fit when the reagent container 90 is installed. At this time, the opening 911 of the container body 91 is sealed by the cap 92, and the dispensing device 10 cannot draw the reagent contained in the reagent container 90. Figure 4 As shown.

[0063] Therefore, to facilitate the dispensing device 10 in drawing reagents from the reagent container 90, the reagent container 90 needs to be pre-treated by the pre-treatment device 83 to at least disengage the container body 91 and the cap 92 from their sealed fit. To facilitate the dispensing operation of the reagent dispensing component 10b of the dispensing device 10 on the reagent container 90, the sample analyzer 100 further performs a cap-opening process on the reagent container 90 by the pre-treatment device 83, making it easier to open the cap 92 and expose the opening 911 so that the reagent dispensing component 10b can reach into the reagent container 90 through the opening 911 to draw reagents from it. Figure 8 As shown.

[0064] In some embodiments, the sample analyzer 100 further includes an information prompting device 84 for providing information prompts. The information prompting device 84 includes at least one of a display component, an indicator light component, and a sound output component. For example, the information prompting device 84 includes a display component, which may be a touch screen, an LCD screen, an LED screen, or an OLED screen, etc. In the event of an abnormal bubble condition in the reagent container 90, the control device 70 controls the display component to display corresponding prompt information on a preset display interface, so that the user can be informed of the abnormal bubble condition in the reagent container 90 through the prompt information displayed by the display component, and thus perform corresponding processing operations.

[0065] For example, the information display device 84 includes an indicator light component. If an abnormality is detected in the reagent container 90 due to air bubbles, the control device 70 controls the indicator light component to display a corresponding color and / or brightness, so that the user is aware of the abnormality in the reagent container 90 through the corresponding color and / or brightness. For example, if an abnormality is detected in the reagent container 90, the indicator light component displays red.

[0066] like Figure 1 As shown, in some embodiments, the control device 70 may be one or more, and the control device 70 may be integrated into any structure, device or component of the sample analyzer 100, or it may be set independently, which is not limited here.

[0067] Optionally, the control device 70 includes at least a processor 701, a memory 702, a communication interface (not shown), and an I / O interface (not shown). The processor 701, memory 702, communication interface, and I / O interface communicate via a bus. The processor 701 can be a Central Processing Unit (CPU), or other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or any conventional processor.

[0068] The memory 702 contains various computer programs, such as the operating system and application programs, for the processor 701 to execute, as well as the data required to execute these programs. During the analysis of the sample under test, any data requiring local storage can be stored in the memory 702. The I / O interface includes, but is not limited to, serial interfaces such as USB, IEEE 1394, or RS-232C; parallel interfaces such as SCSI, IDE, or IEEE 1284; and analog signal interfaces composed of D / A converters and A / D converters. Input components are connected to the I / O interface, allowing users to directly input data to the control device 70. These input components include, but are not limited to, a keyboard, mouse, touchscreen, or control buttons. The display component can communicate with the control device 70 through the I / O interface to provide relevant information prompts. The communication interface can be any known communication protocol. The communication interface communicates with the outside world via a network, and the control device 70 can transmit data with any component connected through the network using a preset communication protocol.

[0069] In some embodiments, the control device 70 is at least used to perform the following operations: specifically, the processor 701 of the control device 70 calls a computer program stored in the memory 702 to perform the following operations: Before the dispensing device 10 draws the reagent from the reagent container 90, the bubble recognition mechanism 81 is controlled to perform a bubble recognition operation on the reagent container 90, and the bubble recognition information output by the bubble recognition mechanism 81 is received. Based on the bubble recognition information, the sample analyzer 100 is controlled to perform bubble pretreatment on the reagent container 90. The bubble pretreatment includes at least one of bubble removal operation and bubble avoidance operation. The control detection device 50 detects the target reaction liquid and obtains the corresponding detection data. The target reaction liquid is formed by mixing the reagent and sample in the reagent container 90 after bubble pretreatment.

[0070] For example, in order to avoid the possibility that air bubbles in the reagent container 90 may cause the dispensing device 10 to draw liquid from the reagent container 90 in the reagent device 30, resulting in inaccurate reagent drawing volume and ultimately affecting the accuracy of sample test results.

[0071] Based on this, before the dispensing device 10 draws reagents from reagent containers 90 in the reagent device 30 (including but not limited to), the control device 70 controls the bubble recognition mechanism 81 to perform bubble recognition on the reagent containers 90, and receives the bubble recognition information output by the bubble recognition mechanism 81. The bubble recognition information can then be analyzed using a preset algorithm to obtain the corresponding bubble recognition result. Furthermore, the control device 70 can determine whether there are any abnormal bubbles in the reagent containers 90 based on the bubble recognition result, and output corresponding control commands to control the sample analyzer 100 to perform the corresponding processing operation.

[0072] The bubble identification result is used to characterize the bubble features corresponding to the bubbles in the reagent container. For example, the bubble identification result includes at least one of the following: the height information of the bubble layer in the reagent container 90, the distribution information of the bubbles, and the morphological information of the bubbles. Furthermore, the morphological information of the bubbles includes at least one of the following: the size of the bubbles and the area of ​​the liquid surface covered by the bubbles. In other words, the bubble features can be understood as including at least one of the following: the height information of the bubble layer in the reagent container 90, the distribution information of the bubbles, the size of the bubbles, and the area of ​​the liquid surface covered by the bubbles.

[0073] An abnormality in the reagent container 90 refers to the presence of air bubbles within the reagent container 90. Due to at least one of the following reasons—the distribution, density, size, or height of the air bubbles within the reagent container 90—the dispensing device 10 may experience either empty or insufficient aspiration during the reagent aspiration process within the reagent container 90.

[0074] For example, bubble anomalies include, but are not limited to, at least one of the following: a bubble layer exists within the reagent container 90 and the height of the bubble layer exceeds a height threshold; bubbles exist within the reagent container 90 and the liquid surface area covered by the bubbles exceeds an area threshold; and bubbles are present at the liquid surface position corresponding to the liquid aspiration performed by the dispensing device 10 within the reagent container 90. The presence of bubbles at the liquid surface position corresponding to the liquid aspiration performed by the dispensing device 10 within the reagent container 90 can also be understood as the liquid surface position covered by bubbles within the reagent container 90 being a preset liquid surface position, and this preset liquid surface position at least includes the liquid surface directly below the opening 911 of the reagent container 90, that is, the liquid surface area covered by the orthographic projection of the opening 911.

[0075] Conversely, if the reagent container 90 contains a bubble layer, but the height of the bubble layer does not exceed the height threshold, then the presence of the bubble layer will not affect the dispensing device 10's performance in reagent aspiration, or the effect will be negligible. Therefore, the reagent container can be considered free of bubble abnormalities. Alternatively, if the reagent container 90 contains bubbles, but the distribution position of the bubbles will not affect the dispensing device 10's performance in reagent aspiration, or the effect will be negligible. Similarly, if the reagent container 90 contains bubbles, but the liquid surface area covered by the bubbles does not exceed the area threshold, and the dispensing device 10 will not affect the performance in reagent aspiration, or the effect will be negligible, then the reagent container can be considered free of bubble abnormalities. Finally, if the reagent container 90 does not contain bubbles, then the reagent container can be considered free of bubble abnormalities.

[0076] In some embodiments, the processing operation includes a first processing operation corresponding to the presence of abnormal bubbles in the reagent container 90 and a second processing operation corresponding to the absence of abnormal bubbles in the reagent container 90. The second processing operation includes moving the reagent container 90 to the placement position 301b of the reagent device 30 so that the dispensing device 10 can draw the reagent from the reagent container 90 placed in the placement position 301b of the reagent device 30 and discharge it into the reaction container, thereby preparing a reaction solution for detection by the detection device 50.

[0077] The first processing operation includes, but is not limited to, bubble pretreatment operation and reagent unloading operation, wherein the reagent unloading operation is used to dispatch the reagent container 90 to a preset position (such as the unloading position) to wait for further processing.

[0078] The bubble pretreatment operation includes a bubble removal operation and a bubble avoidance operation. The bubble removal operation is used to remove or eliminate at least some of the bubbles in the reagent container 90, and the bubble avoidance operation is used to control the pipette of the dispensing device 10 to avoid at least some of the bubbles in the reagent container 90, so as to effectively draw the reagent in the reagent container 90.

[0079] like Figure 9 As shown, after the bubble recognition mechanism 81 performs bubble recognition on the reagent container 90 and obtains bubble recognition information, the control device 70 analyzes the bubble recognition information using a preset algorithm to obtain the corresponding bubble recognition result. Based on the bubble recognition result, the control device 70 controls the sample analyzer 100 to perform a bubble removal operation on the reagent container 90. After the bubble removal operation is completed in the reagent container 90, the dispensing device 10 is controlled to draw the reagent from the reagent container 90 after the bubble removal operation and dispense it into a preset reaction container. This allows the sample to be tested and the reagent to be mixed in the reaction container to form a target reaction solution. The detection component 50 then detects the target reaction solution to obtain the corresponding detection data or detection result of the sample to be tested.

[0080] like Figure 10 As shown, after the bubble recognition mechanism 81 performs bubble recognition operation on the reagent container 90 to obtain bubble recognition information, the control device 70 analyzes the bubble recognition information through a preset algorithm to obtain the corresponding bubble recognition result. Based on the bubble recognition result, the control device 10 avoids at least some of the bubbles in the reagent container 90 and aspirates the reagent in the reagent container 90 to extract the reagent and dispense it into a preset reaction container. This allows the sample to be tested and the reagent to be mixed in the reaction container to form a target reaction solution. The detection component 50 detects the target reaction solution to obtain the corresponding detection data or detection result of the sample to be tested.

[0081] In summary, the bubble pretreatment operation can effectively eliminate or avoid bubbles in the reagent container 90. Therefore, when the dispensing device 10 dispenses reagents into the reagent container 90 after bubble pretreatment, it can effectively reduce the probability of aspirating bubbles, thereby reducing the probability of empty or insufficient aspiration during the reagent aspiration process. This improves the accuracy of reagent aspiration and ultimately enhances the accuracy of the test results.

[0082] In this embodiment, the bubble recognition mechanism 81 performs bubble recognition operation on the reagent container 90 in the following manner: the sample analyzer 100 is provided with a bubble recognition position 62, the bubble recognition mechanism 81 moves to the position of the reagent container 90 to be bubble recognized, and performs bubble recognition operation on the reagent container 90.

[0083] Alternatively, the scheduling device 60 schedules the reagent container 90 to be subjected to bubble recognition operation to a preset position (such as bubble recognition position 62), and the bubble recognition mechanism 81 is set to correspond to the bubble recognition position 62 to perform bubble recognition operation on the reagent container 90 in the bubble recognition position 62.

[0084] Alternatively, the scheduling device 60 schedules the reagent container 90 to be identified to a preset position (such as bubble identification position 62), the bubble identification mechanism 81 moves to the bubble identification position 62, and performs bubble identification operation on the reagent container 90 in the bubble identification position 62. No limitation is made here.

[0085] In some embodiments, before performing bubble pretreatment on the bubbles in the reagent container 90, the control device 90 is further configured to: Based on the bubble recognition result, it is determined whether to perform an unloading operation or bubble pretreatment on the reagent container 90. The unloading operation is used to schedule the reagent container 90 to a preset unloading position.

[0086] For example, the memory 702 stores a first bubble feature corresponding to the execution of an unloading operation and a second bubble feature corresponding to the execution of a bubble pretreatment operation. Based on the bubble identification result, it is determined whether the bubble feature within the reagent container 90 matches the first bubble feature or the second bubble feature. If the bubble identification result indicates that the bubble feature within the reagent container 90 matches the first bubble feature, the sample analyzer 100 is controlled to perform bubble pretreatment on the reagent container 90. If the bubble identification result indicates that the bubble feature within the reagent container 90 matches the second bubble feature, the sample analyzer 100 is controlled to perform an unloading operation on the reagent container 90.

[0087] In some embodiments, the bubble recognition result includes at least the height information of the bubble layer within the reagent container 90. The control device 70 determines, based on the bubble recognition result, whether to perform an unloading operation on the reagent container 90 or to perform bubble pretreatment, including: If the control device 70 confirms that the height of the bubble layer inside the reagent container 90 is greater than the first height threshold based on the height information, the control device 70 controls the scheduling device 60 to perform an unloading operation to schedule the reagent container 90 outside the sample analyzer 100. Alternatively, the control device 70 controls the scheduling device 60 to schedule the reagent container 90 to a preset unloading position (e.g., the reagent unloading position).

[0088] Optionally, if the reagent container 90 is moved outside the sample analyzer 100, or if the reagent container 90 is moved to a preset unloading position, the control information prompting device 84 outputs corresponding prompt information.

[0089] In some embodiments, the bubble recognition result includes at least the height information of the bubble layer within the reagent container 90. Based on the bubble recognition result, the control device 70 determines whether to perform an unloading operation on the reagent container 90 or to perform bubble pretreatment, including: If the control device 70 confirms, based on the height information, that the height of the bubble layer inside the reagent container 90 is greater than a second height threshold and less than or equal to a first height threshold, it determines to perform bubble pretreatment on the reagent container 90, wherein the first height threshold is greater than the second height threshold.

[0090] Optionally, if the control device 70 confirms that the height of the bubble layer inside the reagent container 90 is greater than the second height threshold and less than or equal to the first height threshold based on the height information, the control device 70 controls the scheduling device 60 to schedule the reagent container 90 to the bubble removal position 63, and controls the bubble removal device 82 of the sample analyzer 100 to perform a bubble removal operation on the reagent container 90 located at the bubble removal position 63.

[0091] In some embodiments, the control device 70 is further configured to, upon confirming based on height information that the height of the bubble layer inside the reagent container 90 is less than or equal to a second height threshold, control the scheduling device 60 to schedule the reagent container 90 to the target placement position of the reagent device 30, wherein the first height threshold is greater than the second height threshold.

[0092] Optionally, the target placement position is at least one of the placement positions 301b, and is used to store the reagent container 90 that has no bubble abnormality after bubble detection, and / or to store the reagent container 90 that has been treated by bubble removal operation, and / or to perform bubble avoidance operation.

[0093] Optionally, after confirming that the height of the bubble layer inside the reagent container 90 is less than or equal to the second height threshold based on the height information, and after the control scheduling device 60 schedules the reagent container 90 to the target placement position of the reagent device 30, the control device 70 is further configured to: Based on the height information, reagent aspiration control parameters for controlling the aspiration of reagent by the dispensing device 10 are determined. The reagent aspiration control parameters include at least the insertion depth of the pipette of the dispensing device 10 into the reagent container and / or the reagent aspiration rate of the pipette of the dispensing device 10 for aspiration. The dispensing device 10 is controlled to perform reagent aspiration operation on the reagent container 90 according to the reagent aspiration control parameters, and the aspirated reagent is discharged into the reaction container so that the reagent and sample in the reagent container 90 are mixed to form the target reaction solution; The control and detection device 50 detects the target reaction solution and obtains the corresponding detection data.

[0094] For example, if the control device 90 confirms after analyzing the bubble identification information that the height of the bubble layer in the reagent container 90 exceeds a first height, and if the time required for the bubble removal device 82 to remove the bubbles in the reagent container 90 is too long, or if the bubble removal effect may be poor, then the control scheduling device 60 will schedule the reagent container 90 to be outside the sample analyzer 100, or the control device 70 will control the scheduling device 60 to schedule the reagent container 90 to a preset unloading position, and the information prompting device 84 will output corresponding prompt information to prompt the user to remove the bubbles manually, so as to ensure the bubble removal effect in the reagent container 90.

[0095] If the height of the bubble layer in the reagent container 90 is greater than the second height threshold and less than or equal to the first height threshold, the bubble removal device 82 can remove the bubbles in the reagent container 90 to reduce at least some of the bubbles in the reagent container 90, thereby reducing the amount or density of bubbles in the reagent container 90, which facilitates the dispensing device 30 to draw the reagent.

[0096] When the height of the bubble layer inside the reagent container 90 is less than or equal to the second height threshold, the bubbles inside the reagent container 90 have a low, or even negligible, impact on the dispensing device 10's absorption of the reagent inside the reagent container 90. Therefore, the control scheduling device 60 schedules the reagent container 90 to the target placement position of the reagent device 30 so that the dispensing device 10 can absorb the reagent from the reagent container 90 placed in the placement position 301b and discharge it into the reaction container, thereby preparing the corresponding reaction solution for the detection device 50 to detect.

[0097] Furthermore, when the height of the bubble layer inside the reagent container 90 is less than or equal to the second height threshold, in order to improve the absorption effect of the dispensing device 10 on the reagent inside the reagent container 90, reagent absorption control parameters for controlling the absorption of reagent by the dispensing device 10 are determined based on the height information. The reagent absorption control parameters include at least the insertion depth of the pipette of the dispensing device 10 below the liquid surface of the reagent container 90 and / or the reagent absorption speed of the pipette of the dispensing device 10 when absorbing reagent below the liquid surface. Thus, the dispensing device 10 can be controlled to perform reagent absorption operation on the reagent container 90 according to the reagent absorption control parameters, so as to ensure that the pipette 10 absorbs reagent below the liquid surface, avoids empty or insufficient reagent absorption, improves the accuracy of reagent absorption, and ensures that the dispensing device 10 can absorb an accurate amount of reagent during the reagent absorption process of the reagent container 90, so as to ensure that there is enough reagent to mix with the sample to be tested to form the target reaction solution. Finally, when the detection device 50 detects the target reaction solution, it can obtain more accurate detection data.

[0098] It is understood that the first height threshold and the second height threshold can be set as needed. For example, the first height threshold is 6mm and the second height threshold is 2mm, or the first height threshold is 5mm and the second height threshold is 1mm, or the first height threshold is 5mm and the second height threshold is 0.5mm, etc., and no limitation is made here.

[0099] like Figure 11 As shown, for example, after analyzing the bubble identification information, the control device 90 confirms that the height of the bubble layer in the reagent container 90 is H2. If H2 is greater than the first height threshold, the control device 70 controls the scheduling device 60 to schedule the reagent container 90 to the preset unloading position.

[0100] like Figure 12 As shown, if H2 is greater than the second height threshold and less than or equal to the first height threshold, the bubble removal device 82 and reagent container 90 are controlled to perform a bubble removal operation.

[0101] like Figure 13 As shown, if H2 is less than or equal to the second height threshold, the control scheduling device 60 will schedule the reagent container 90 to the target placement position of the reagent device 30.

[0102] In some embodiments, the bubble recognition result includes at least the morphological information and / or the distribution information of the bubbles within the reagent container 90. The control device 70 determines, based on the bubble recognition result, whether to perform an unloading operation on the reagent container 90 or to perform bubble pretreatment, including: If a bubble avoidance operation is determined to be performed on reagent container 90 based on the bubble recognition result, the reagent scheduling device 60 is controlled to schedule reagent container 90 located at bubble recognition position 62 to the target placement position of reagent device 30. Based on the bubble recognition information, the dispensing device 10 is controlled to avoid at least some of the bubbles in the reagent container 90 located at the target placement position, and to perform a liquid aspiration operation on the reagent in the reagent container 90. The dispensing device 10 controls the dispensing of the aspirated reagent into the reaction container 90 so that the reagent and sample in the reagent container 90 are mixed to form the target reaction solution, and controls the detection device 50 to detect the target reaction solution to obtain the corresponding detection data.

[0103] In some embodiments, the bubble recognition result includes at least the morphological information and / or the distribution information of the bubbles within the reagent container 90. The control device 70 determines, based on the bubble recognition result, whether to perform an unloading operation on the reagent container 90 or to perform bubble pretreatment, including: When it is determined that a bubble removal operation should be performed on the reagent container 90 based on the bubble recognition information, the reagent scheduling device 60 is controlled to schedule the reagent container 90 located at the bubble recognition position 62 to the bubble removal position 63, and the bubble removal device 82 is controlled to perform a bubble removal operation on the reagent container 90 located at the bubble removal position 63. After the reagent container 90 completes the bubble removal operation, the reagent scheduling device 60 controls the reagent container 90 located at the bubble removal position 63 to the target placement position of the reagent device 30. The reagent in the reagent container 90 at the target placement position is controlled by the dispensing device 10 and discharged into the reaction container so that the reagent and the sample to be tested are mixed to form the target reaction solution. The detection device 50 is controlled to detect the target reaction solution and obtain the corresponding detection data.

[0104] Please see Figures 14 to 15Based on the morphological and / or distribution information of bubbles in the reagent container 90, if it is determined that there is an abnormality in the bubble in the reagent container 90, and if the type of abnormality is determined to be consistent with the liquid aspiration operation performed by the dispensing device 10 (such as the reagent dispensing component 10b), the dispensing device 10 is controlled to avoid at least some of the bubbles in the reagent container 90 located at the target placement position and to perform a liquid aspiration operation on the reagent in the reagent container 90, so as to effectively reduce the risk of empty aspiration or insufficient aspiration caused by the dispensing device 10 aspirating liquid on the liquid surface where the bubble area of ​​the reagent container 90 is located, improve the liquid aspiration accuracy of the dispensing device 10, and after the dispensing device 10 completes the liquid aspiration, the dispensing device 10 is controlled to discharge the aspirated reagent into the reaction container so that the reagent and sample in the reagent container 90 are mixed to form the target reaction solution, and the detection device 50 is controlled to detect the target reaction solution to obtain the corresponding detection data.

[0105] It is understandable that determining whether the abnormal bubble type matches the liquid aspiration operation performed by the dispensing device 10 (such as the reagent dispensing component 10b) based on morphological information and / or bubble distribution information can be done by judging whether the area without bubbles or the area with sparse bubbles in the reagent container 90 is located directly below the opening 911. If the area without bubbles or the area with sparse bubbles is located directly below the opening 911, it indicates that controlling the pipette of the dispensing device 10 to aspirate liquid from the area without bubbles or the area with sparse bubbles can effectively reduce or even avoid empty aspiration or under-aspiration by the pipette, thereby effectively improving the accuracy of reagent aspiration.

[0106] like Figure 14 and Figure 15 As shown, air bubbles exist on the liquid surface of the aspiration area corresponding to the liquid aspiration operation performed by the dispensing device 10 within the reagent container 90. However, the number of air bubbles is small, or the distribution of air bubbles may not cover the entire aspiration area. Liquid aspiration can be performed by avoiding air bubbles. Normally, when the dispensing device 10 aspirates reagent from the reagent container 90, the area directly below the opening of the reagent container 90 is the aspiration area. If there are no abnormal air bubbles on the liquid surface of the aspiration area corresponding to the liquid aspiration operation performed by the dispensing device 10 within the reagent container 90, it indicates that the impact on the reagent aspiration of the dispensing device 10 is negligible. The reagent container 90 can be moved to the reagent device 30, and liquid aspiration can be performed by avoiding air bubbles.

[0107] Please see Figure 16If, based on the morphological and / or distribution information of bubbles within the reagent container 90, it is determined that the reagent container 90 has an abnormality related to bubbles, and the type of bubble abnormality requires bubble removal based on the morphological and / or distribution information, that is, if the area within the reagent container 90 without bubbles or the area with sparse bubbles is not directly below the opening 911, it indicates that the pipette of the dispensing device 10 cannot be controlled to avoid the bubbles during aspiration, and bubble removal is necessary to reduce the amount of bubbles in the reagent container 90. In this case, the reagent container 90 can be moved to the bubble removal position 63 by the control scheduling device 60, and the bubble removal device 82 can be controlled to perform a bubble removal operation on the reagent container 90 located at the bubble removal position 63, thereby effectively reducing the number of bubbles in the reagent container 90.

[0108] like Figure 16 The area directly below the opening of the reagent container 90 is the aspiration area. If there are abnormal air bubbles at the liquid surface position of the aspiration area corresponding to the liquid aspiration performed by the dispensing device 10 inside the reagent container 90, the air bubble avoidance operation cannot be performed. At this time, the reagent container 90 can be moved to the air bubble removal position 63 to perform the air bubble removal operation.

[0109] Please see Figure 17 In some embodiments, the bubble removal device 82 includes an ultrasonic component 821, and the control device 70 performs a bubble removal operation on the reagent container 90 located at the bubble removal position 63, including controlling the ultrasonic component 821 to output ultrasonic waves to the bubble layer in the reagent container 90 located at the bubble removal position 63 to remove at least a portion of the bubbles in the reagent container 90.

[0110] like Figure 17 As shown, exemplarily, after the scheduling device 60 schedules the reagent container 90 to be subjected to bubble removal operation to the bubble removal position 63, the ultrasonic component 821 is controlled to emit ultrasonic waves toward the reagent container 90 located at the bubble removal position 63, so as to remove at least part of the bubbles in the reagent container 90 by ultrasonic vibration.

[0111] Please see Figure 18 In some embodiments, the bubble removal device 82 includes a pressure component 822, and the control device 70 controls the bubble removal device 82 to perform a bubble removal operation on the reagent container 90 located at the bubble removal position 63, including: controlling the pressure component 822 to change the air pressure inside the reagent container 90 to remove at least a portion of the bubbles inside the reagent container 90.

[0112] like Figure 18As shown, exemplarily, after the scheduling device 60 schedules the reagent container 90 to be subjected to bubble removal operation to the bubble removal position 63, the control pressure component 822 seals the opening 911 of the reagent container 90 to perform a pressurization or depressurization operation into the reagent container 90, so as to change the air pressure inside the reagent container 90, thereby causing the air pressure inside and outside the bubble to become unbalanced, causing the bubble to burst, so as to remove at least part of the bubble in the reagent container 90.

[0113] Please see Figure 19 In some embodiments, the bubble removal device 82 is a dispensing device 10, and the control device 70 controls the bubble removal device 82 to perform a bubble removal operation on the reagent container 90 located at the bubble removal position 63, including: controlling the dispensing device 10 to perform a bubble removal operation on the reagent container 90 located at the bubble removal position 63 to remove at least a portion of the bubbles in the reagent container 90.

[0114] For example, the control device 70 controls the dispensing device 10 to perform a bubble removal operation on the reagent container 90 located at the bubble removal position 63, including: controlling the dispensing device 10 to perform a liquid aspiration operation on the reagent container 90 located at the bubble removal position 63 according to the bubble identification result, so as to remove the bubbles in the reagent container 90; after the liquid aspiration operation is completed, controlling the dispensing device 10 to move to the drain position and perform a drain operation.

[0115] like Figure 19 As shown, exemplarily, the bubble removal device 82 can reuse the dispensing device 10. By controlling the needle moving mechanism, the pipette is moved to the reagent container 90 located at the bubble removal position 63, and the power mechanism is controlled to provide power to the pipette so that the pipette can perform aspiration operation, thereby causing the bubbles to burst by aspirating them. After the aspiration operation is completed, the needle moving mechanism drives the pipette to the cleaning device of the sample analyzer 100 for drainage or to a preset reaction container for drainage.

[0116] It is understood that the bubble recognition position 62 and the bubble removal position 63 can be set as needed. For example, at least one of the bubble recognition positions 62 and the bubble removal position 63 can be set in the reagent device 30. That is, the bubble recognition position 62 and / or the bubble removal position 63 can be implemented by reusing the placement position 301b, or the corresponding bubble recognition position 62 and bubble removal position 63 can be set in the reagent device 30.

[0117] In some embodiments, the dispensing device 10 is controlled to perform a liquid aspiration operation on the reagent container 90 located at the bubble removal position based on the bubble recognition result, including: A liquid aspiration control strategy is determined based on the bubble recognition result and a preset first association relationship. The first association relationship records the bubble recognition result and the liquid aspiration control strategy associated with the bubble recognition result. The dispensing device 10 is controlled to perform a liquid aspiration operation on the reagent container 90 located at the bubble removal position according to the liquid aspiration control strategy. The liquid aspiration control strategy includes at least one of the following: the target number of liquid aspiration operations performed by the dispensing device 10, the single liquid aspiration volume corresponding to each liquid aspiration operation performed by the dispensing device 10, the total liquid aspiration volume corresponding to the liquid aspiration operation performed by the dispensing device 10, the liquid aspiration speed of the pipette during the liquid aspiration operation performed by the dispensing device 10, and the liquid aspiration height of the pipette during the liquid aspiration operation performed by the dispensing device 10.

[0118] In some embodiments, the aspiration control strategy includes the aspiration height of the pipette needle during the aspiration operation of the dispensing device 10; the dispensing device 10 includes a pipette needle, a needle moving mechanism, and a power mechanism, wherein the needle moving mechanism is used to drive the pipette needle to move; and the power mechanism is used to provide power for the pipette needle to aspirate and dispense the target liquid, the target liquid including at least a reagent. The control device 70 controls the dispensing device 10 to perform a liquid aspiration operation on the reagent container 90 located at the bubble removal position according to the liquid aspiration control strategy, including: The liquid level of the reagent in the reagent container 90 is determined based on the bubble recognition results; The control needle moving mechanism moves the pipette to the first preset position inside the reagent container 90 according to the aspiration height and liquid level height, so that the distance between the tip of the pipette and the reagent liquid surface matches the aspiration height. After the pipette moves to the first preset position, or during the process of the pipette moving to the preset position, the control power mechanism drives the pipette to perform the liquid aspiration action.

[0119] like Figure 19 As shown, exemplarily, the bubble recognition result includes at least one of the following: bubble layer height information, bubble distribution information, and bubble morphology information within the reagent container 90. The control device 70 determines the aspiration height corresponding to the aspiration control strategy as Δh based on the bubble recognition result. That is, the bubble recognition result determines that the distance between the pipette and the reagent liquid surface within the reagent container 90 during the aspiration operation of the dispensing device 10 on the reagent in the reagent container 90 is Δh. Based on the bubble recognition result, the liquid surface height of the reagent liquid in the reagent container 90 is determined, and the needle moving mechanism is controlled to move the pipette to a position within the reagent container 90 at a distance of Δh from the reagent liquid surface. The power mechanism is then controlled to drive the pipette to perform the aspiration action to eliminate some of the bubbles within the reagent container 90.

[0120] In some embodiments, the aspiration control strategy includes a target number of aspiration operations performed by the dispensing device 10; the dispensing device 10 includes a pipette, a needle moving mechanism, and a power mechanism, wherein the needle moving mechanism is used to move the pipette; the power mechanism is used to provide power for the pipette to aspirate and expel the target liquid, the target liquid including at least a reagent; controlling the dispensing device 10 to perform aspiration operations on the reagent container 90 located at the bubble removal position according to the aspiration control strategy includes: controlling the dispensing device 10 to perform the target number of aspiration operations on the reagent container 90, wherein each aspiration operation includes: controlling the needle moving mechanism to move the pipette to a preset aspiration position within the reagent container 90, and controlling the power mechanism to drive the pipette to perform the aspiration action; after the aspiration action is completed, controlling the needle moving mechanism to move the pipette to a preset discharge position and controlling the power mechanism to drive the pipette to perform the discharge action.

[0121] Optionally, the target number of times is N, and N≥2, wherein during the N liquid aspiration operations, the distance between the tip of the pipette and the reagent liquid surface of the reagent container 90 is the same each time the pipette performs the liquid aspiration action.

[0122] Alternatively, during N aspiration operations, when the pipette performs the aspiration action for the (N-1)th time, the distance between the tip of the pipette and the reagent surface of the reagent container 90 is the first distance, and when the pipette performs the aspiration action for the Nth time, the distance between the tip of the pipette and the reagent surface of the reagent container 90 is the second distance. The first distance is greater than the second distance so that the amount of reagent aspirated by the pipette during the aspiration process is less.

[0123] Optionally, the target number of times is N, and N≥2, wherein during the N aspiration operations, the volume of liquid aspirated by the pipette for the Nth aspiration action is less than the volume of liquid aspirated by the (N-1)th aspiration action; and / or, the aspiration speed of the pipette for the Nth aspiration action is less than the aspiration speed of the (N-1)th aspiration action, so that the pipette aspirates less reagent during the aspiration process.

[0124] like Figure 19 As shown, for example, the control device 70 determines the liquid aspiration control strategy based on the bubble recognition result as performing N liquid aspiration operations in the reagent container. That is, the target number of liquid aspiration operations performed by the dispensing device 10 is N times, where N≥1. For example, N can be 1, 2, 3, or 4, and there is no limitation here.

[0125] For example, if the height (thickness) of the bubble layer is determined to be in the first height range based on the bubble recognition result, it is determined that the dispensing device 10 needs to perform one liquid aspiration operation on the bubble layer. If the height (thickness) of the bubble layer is determined to be in the second height range based on the reagent image data, it is determined that the dispensing device 10 needs to perform at least two liquid aspiration operations on the bubble layer.

[0126] When N≥2, when it is necessary to remove air bubbles from reagent container 90, the dispensing device 10 is controlled to repeatedly perform liquid aspiration operation on reagent container 90 N times. Each liquid aspiration operation includes controlling the needle moving mechanism to move the pipette to the reagent container 90 located at the air bubble removal position 63 and at a preset distance (e.g., Δh) from the reagent liquid surface, and controlling the power mechanism to drive the pipette to perform the liquid aspiration action. After the liquid aspiration action is completed, the needle moving mechanism is controlled to move the pipette to the preset liquid discharge position and the power mechanism is controlled to drive the pipette to perform the liquid discharge action.

[0127] It is understandable that during the N sampling operations performed by the dispensing device 10, the distance between the tip of the pipette and the reagent surface for each sampling operation can be the same or different. Similarly, the pipette aspiration rate or the volume of liquid aspirated per unit time for each sampling operation during the N sampling operations performed by the dispensing device 10 can be the same or different.

[0128] like Figure 20 As shown, in some embodiments, the bubble layer within the reagent container 90 includes a first type of bubble layer x1 and a second type of bubble layer x2. The first type of bubble layer x1 is located above the reagent liquid surface of the reagent container 90, and the second type of bubble layer x2 is located on the side of the first type of bubble layer x1 away from the reagent liquid surface. The bubble density of the second type of bubble layer x2 is less than the bubble density of the first type of bubble layer x1. The aspiration control strategy includes the aspiration speed of the pipette needle during the aspiration operation performed by the dispensing device 10. Controlling the dispensing device 10 to perform an aspiration operation on the reagent container 90 located at the bubble removal position according to the aspiration control strategy includes: The dispensing device 10 is controlled to perform at least one first aspiration operation on the second type of bubble layer x2, and the aspiration speed of the pipette needle during the first aspiration operation is the first aspiration speed. After the first liquid aspiration operation is completed, the dispensing device 10 is controlled to perform a second liquid aspiration operation on the second type of bubble layer x2, and the liquid aspiration speed of the pipette needle during the second liquid aspiration operation is the second liquid aspiration speed, wherein the first liquid aspiration speed is greater than the second liquid aspiration speed.

[0129] For example, generally, the closer the bubble layer is to the surface of the reagent liquid, the greater the density of the bubble layer. The greater the density of the bubble layer, the greater the amount of reagent carried away by the pipette during the same aspiration time. Therefore, in order to minimize the loss of reagent in the reagent container 90, different aspiration speeds are used for reagent layers of different densities in this embodiment.

[0130] Please see Figure 21 In some embodiments, the bubble recognition mechanism 81 includes an image acquisition mechanism 81a, and the control device 70 controls the bubble recognition mechanism 81 to perform a bubble recognition operation on the reagent container 90, including: The image acquisition mechanism 81a is controlled to acquire images of the reagents stored in the storage chamber and uses the reagent image data as bubble recognition information.

[0131] Optionally, the control device 70 controls the image acquisition mechanism 81a to acquire images of the reagents stored in the storage chamber in the following ways: The control and scheduling device 60 schedules the reagent container 90 to the bubble recognition position 62 and controls the image acquisition mechanism 81a to acquire images of the reagents stored in the reagent container 90 located in the bubble recognition position 62, and uses the reagent image data as bubble recognition information.

[0132] For example, the image acquisition mechanism 81a is set to correspond to the bubble recognition position 62. After the reagent container 90 is scheduled to the bubble recognition position 62, the image acquisition mechanism 81a performs image acquisition on the reagent stored in the reagent container 90 located in the bubble recognition position 62 to obtain reagent image data.

[0133] Alternatively, the image acquisition mechanism 81a can move in two-dimensional or three-dimensional space. After the reagent container 90 is scheduled to the bubble recognition position 62, the image acquisition mechanism 81a moves to the bubble recognition position 62 and performs image acquisition on the reagent stored in the reagent container 90 located in the bubble recognition position 62 to obtain reagent image data.

[0134] In some embodiments, the image acquisition mechanism 81a includes a light source assembly 811 for emitting light signals and a signal acquisition assembly 812 for acquiring light signals, and the light source assembly 811 and the signal acquisition assembly 812 are respectively disposed on opposite sides of the bubble recognition position 62 in the horizontal direction. The control device 70 is further used for: The control and scheduling device 60 schedules the reagent container 90 to the bubble recognition position and controls the light source assembly 811 to emit a first light signal to the reagent container 90 located at the bubble recognition position 62. The receiving signal acquisition component 812 outputs reagent image data based on the acquired second optical signal, wherein the second optical signal includes the optical signal formed by the first optical signal emitted from the reagent container.

[0135] like Figure 21As shown, exemplarily, when the reagent container 90 needs to be detected for bubbles, the control scheduling device 60 schedules the reagent container 90 to be identified for bubble detection to the bubble detection position 62, and controls the light source component 811 to emit a first light signal to the reagent container 90 located at the bubble detection position 62, so that the first light signal passes through the reagent container 90 and is emitted to the signal acquisition component 812. Based on the different absorption, scattering, and refraction capabilities of the first light signal in the bubble-containing area and the bubble-free area within the reagent container 90, the signal acquisition component 812 collects the light signal emitted through the reagent container 90 and converts it into corresponding reagent image data, and transmits the reagent image data as bubble detection information to the control device 70. Thus, the control device 70 analyzes the bubble detection information to obtain the corresponding bubble detection result.

[0136] like Figure 22 As shown, after receiving reagent image data as bubble identification information, the control device 70 analyzes the bubble identification information to obtain at least one of the following: bubble layer height information, bubble distribution information, and bubble morphology information within the reagent container 90. For example, by analyzing the reagent image data, it can be determined that bubbles exist in the currently measured reagent container 90, and the bubble layer height is H, wherein the height of the dense bubble layer (first type of bubble layer) is x2, and the height of the sparse bubble layer (second type of bubble layer) is x1.

[0137] Please see Figure 23 In some embodiments, the image acquisition mechanism 81a includes a light source assembly 811 for emitting light signals and a signal acquisition assembly 812 for acquiring light signals. The signal acquisition assembly 812 and the light source assembly 811 are disposed on the same side of the reagent container 90 corresponding to the opening 911, or the signal acquisition assembly 812 is disposed corresponding to the opening 911 of the reagent container 90, and the light source assembly 811 is disposed on the side of the reagent container 90 adjacent to the opening 911. The control device 70 is further configured to: The control and scheduling device 60 schedules the reagent container 90 to the bubble recognition position 62 and controls the light source assembly 811 to emit a first light signal to the reagent container 90 located at the bubble recognition position 62; the receiving signal acquisition assembly 812 outputs reagent image data based on the acquired second light signal, wherein the second light signal includes the reflected light signal of the first light signal reflected by the liquid surface of the reagent container 90.

[0138] For example, when the reagent container 90 needs to be detected for bubbles, the control scheduling device 60 schedules the reagent container 90 to be identified for bubble recognition to the bubble recognition position 62, and controls the light source component 811 to emit a first light signal to the reagent container 90 located at the bubble recognition position 62, so that the first light signal passes through the reagent container 90 and is emitted to the signal acquisition component 812. Based on the different reflection capabilities of the first light signal in the bubble-containing area and the bubble-free liquid surface area in the reagent container 90, the signal acquisition component 812 collects the reflected light signal reflected from the liquid surface of the reagent container 90 and converts it into corresponding reagent image data. The reagent image data is then transmitted to the control device 70 as bubble recognition information. The control device 70 then analyzes the bubble recognition information to obtain the corresponding bubble recognition result.

[0139] like Figure 4 and Figure 5 As shown, in some embodiments, the bubble detection mechanism 81 includes the pipette, and the control device 70 is further used for: The control and scheduling device 60 schedules the reagent container 90 to the bubble recognition position 63; The control needle moving mechanism drives the pipette to move from a preset position to the reagent container 90 located at the bubble recognition position 60, and acquires the bubble recognition information generated by the bubble recognition mechanism 81 based on the detected electrical information of the pipette during the movement of the pipette.

[0140] For example, the electrical characteristics of a pipette may change when it comes into contact with a target object, which may include at least air bubbles. The bubble recognition mechanism 81 includes a pipette and a needle signal processing unit. The needle signal processing unit is connected to the pipette and is used to sample the changes in the electrical signal of the pipette to output corresponding bubble recognition information. For example, when the pipette comes into contact with a liquid surface, air bubbles, or other target objects, its capacitance characteristics change. The needle signal processing unit collects the capacitance characteristics of the pipette and converts them into corresponding electrical signals (such as current signals, voltage signals, or frequency signals), thereby outputting corresponding bubble recognition information.

[0141] Based on the different changes in capacitance characteristics when a pipette contacts the liquid surface and when it contacts an air bubble, by detecting the change in capacitance characteristics of the pipette as it descends towards the liquid surface in the reagent container 90, information such as the presence of air bubbles in the reagent container 90, the thickness of the air bubble layer in the reagent container 90, and the distribution of air bubbles in the reagent container 90 can be detected.

[0142] like Figure 4 and Figure 5As shown, the capacitance characteristic of the pipette is detected starting at time T0. During the time interval from T0 to T1, the pipette does not contact the air bubble. At time T1, it contacts the air bubble, and the capacitance characteristic of the pipette undergoes a first state change. During the time interval from T1 to T2, the pipette continuously contacts the air bubble. At time T2, it begins to contact the liquid surface, and the capacitance characteristic of the pipette undergoes a second state change. For example, through... Figure 5 Analysis shows that there are air bubbles above the liquid surface area contacted by the pipette, and the height of the bubble layer is H = (T2 - T1)·S, where S is the descent speed of the pipette during the time period from T1 to T2.

[0143] Please refer to the following: Figure 2 In some embodiments, the sample analyzer 100 further includes a reagent loading device 80, which has multiple loading positions 801 for holding reagent containers 90. The reagent loading device 80 includes, but is not limited to, a reagent tray. For example, when a test reagent container 90 is loaded, it is placed in the reagent loading device 80 for buffering and awaiting bubble detection.

[0144] In some embodiments, the control device 100 is further configured to: control the scheduling device 60 to schedule the reagent container 90 located in the reagent loading device 80 to the bubble recognition position 62, and control the bubble recognition mechanism 81a to perform bubble recognition operation on the reagent container 90 located in the bubble recognition position 62, and output the corresponding bubble recognition information; If it is determined from the bubble identification information that there is no bubble abnormality in the reagent container 90, the control scheduling device 60 schedules the reagent container 90 to the target placement position of the reagent device 30. If the reagent container 90 is found to have an abnormality due to bubbles based on the bubble identification information, the bubble removal device 82 is controlled to perform bubble pretreatment on the reagent container 90. The bubble pretreatment includes at least one of bubble removal operation and bubble avoidance operation.

[0145] Optionally, the control device 70 is further configured to: when the reagent container 90 is detected to be loaded into the preset loading position of the reagent loading device 80, control the scheduling device 60 to schedule the reagent container 90 located in the preset loading position to the bubble recognition position 62, and control the bubble recognition mechanism 81a to perform bubble recognition operation on the reagent container 90 located in the bubble recognition position 62, and output the corresponding bubble recognition information.

[0146] For example, the preset loading position is at least one of the loading positions 801 of the reagent loading device 80. After the reagent container 90 is loaded into the preset loading position, the scheduling device 60 schedules the reagent container 90 located at the preset loading position to the bubble recognition position 62, and controls the bubble recognition mechanism 81a to perform bubble recognition operation on the reagent container 90 located at the bubble recognition position 62 and output the corresponding bubble recognition information. Thus, the control device 70 can analyze the bubble recognition information to determine whether there is a bubble abnormality in the reagent container 90.

[0147] If the bubble identification information determines that there is no bubble abnormality in the reagent container 90, the control scheduling device 60 will schedule the reagent container 90 to the target placement position of the reagent device 30.

[0148] If the bubble identification information determines that the reagent container 90 has an abnormality, the control scheduling device 60 schedules the reagent container 90 to the bubble removal position 63, and controls the bubble removal device 82 to perform bubble removal operation on the reagent container 90 located at the bubble removal position 63. After the bubble removal operation is completed, the control scheduling device 60 schedules the reagent container 90 that has completed the bubble removal operation to the target placement position.

[0149] Alternatively, if the bubble identification information indicates that there is an abnormality in the reagent container 90, the control scheduling device 60 schedules the reagent container 90 to a preset placement position (such as the target placement position), and controls the dispensing device 10 to perform a bubble avoidance operation on the reagent container 90 to draw the reagent from the reagent container 90 located at the preset placement position and discharge it into the reaction container, so that the sample to be tested and the reagent are mixed in the reaction container to form the target reaction solution.

[0150] If the bubble identification information indicates that there is an abnormality in the reagent container 90, the control scheduling device 60 schedules the reagent container 90 to the bubble removal position 63 and controls the bubble removal device 82 to perform bubble removal operation on the reagent container 90 located at the bubble removal position 63. After the bubble removal operation is completed, the bubble identification mechanism 81a performs a bubble identification operation again. The control device 70 analyzes the bubble identification information collected by the bubble identification mechanism 81a again and performs the corresponding processing operation until the bubbles in the reagent container 90 meet the preset requirements. Then, the control scheduling device 60 schedules the reagent container 90, which has completed the bubble removal operation and meets the preset requirements, to the target placement position.

[0151] It is understood that the method for detecting whether the reagent container 90 has been loaded into the preset loading position can be by setting a sensor and detecting whether the sensor is triggered to determine whether the reagent container 90 has been loaded into the preset loading position. The sensor can be set inside the preset loading position, or around the preset loading position, or on the path on which the reagent container 90 is scheduled to the preset loading position. There is no limitation here.

[0152] In some embodiments, the bubble detection mechanism 81a is disposed in the reagent loading device 80 or in the reagent loading port of the reagent loading device 80, wherein the bubble detection position 62 is disposed corresponding to the bubble detection mechanism 81a and is located in the reagent loading device 80. For example, the bubble detection mechanism 81a is disposed inside the reagent loading device 80, so that bubble detection can be quickly performed on the reagent container 90 after it is loaded into the reagent loading device 80.

[0153] In some embodiments, the bubble recognition mechanism 81a is disposed on the reagent device 30, and the bubble recognition position 62 is disposed corresponding to the bubble recognition mechanism 81a and located on the reagent device 30. For example, the bubble recognition mechanism 81a is disposed above the corresponding placement position 301b of the reagent device 30 or on opposite sides in the horizontal direction.

[0154] In some embodiments, the bubble recognition mechanism 81a is disposed on the reagent container 90 scheduling path between the reagent loading device 80 and the reagent device 30, and the bubble recognition position 62 is disposed corresponding to the bubble recognition mechanism 81a and located on the reagent container 90 scheduling path, so as to facilitate bubble detection during the scheduling process of the reagent container 90.

[0155] like Figure 2 As shown, in some embodiments, the sample analyzer 100 further includes a pretreatment device 83, which is used to release the sealing fit between the container body 92 and the cap 91 so that the dispensing device 10 can draw reagents from the reagent container 90; the control device 70 is also used for: Before the bubble recognition mechanism 81a performs bubble recognition on the reagent stored in the storage cavity 912, the pretreatment device 83 pretreatments the reagent container 90 to at least release the sealing fit between the container body 92 and the cap 91.

[0156] Optionally, the bubble recognition mechanism 81a is disposed in the pretreatment device 83.

[0157] For example, after the reagent container 90 is loaded into the preset loading position, the scheduling device 60 schedules the reagent container 90 located in the preset loading position to the pre-processing position 64. The pre-processing device 83 pre-processes the reagent container 90 located in the pre-processing position to at least release the sealing fit between the container body 92 and the cap 91. The scheduling device 60 schedules the pre-processed reagent container 90 to the bubble recognition position 62 and controls the bubble recognition mechanism 81a to perform bubble recognition operation on the reagent container 90 located in the bubble recognition position 62 and output the corresponding bubble recognition information. Thus, the control device 70 can analyze the bubble recognition information to determine whether there is a bubble abnormality in the reagent container 90. If the bubble recognition information determines that the reagent container 90 is abnormal, the control device 70 can analyze the bubble recognition information to determine whether there is a bubble abnormality in the reagent container 90. If there is no bubble abnormality in reagent container 90, the control scheduling device 60 will schedule reagent container 90 to the target placement position of reagent device 30. If it is determined that there is a bubble abnormality in reagent container 90 based on bubble identification information, the control scheduling device 60 will schedule reagent container 90 to bubble removal position 63 and control bubble removal device 82 to perform bubble removal operation on reagent container 90 located at bubble removal position 63. After the bubble removal operation is completed, the control scheduling device 60 will schedule reagent container 90 that has completed the bubble removal operation to the target placement position of reagent device 30, so that dispensing device 10 (e.g., reagent dispensing component 10b) can perform reagent dispensing operation on reagent container 90 located at the target placement position.

[0158] In some embodiments, the pretreatment device 83 is also used to open the reagent container 90 in the target placement position of the reagent device 30, so as to expose the opening 911 of the reagent container 90, so that the dispensing device 10 can perform a reagent dispensing operation on the reagent container 90 in the target placement position through the opening 911 of the reagent container 90, such as Figure 8 As shown.

[0159] It is understood that, in the embodiments of this application, at least two of the bubble recognition position 62, bubble removal position 63, and pretreatment position 64 can be provided in the same structure, component, or device of the sample analyzer 100. Alternatively, the bubble recognition position 62, bubble removal position 63, and pretreatment position 64 can be provided in different structures, components, or devices, which is not limited here.

[0160] Furthermore, if at least two of the bubble recognition position 62, bubble removal position 63, and preprocessing position 64 can be set in the same structure, component, or device of the sample analyzer 100, the same position can be used as the bubble recognition position 62, bubble removal position 63, or preprocessing position 64 by time-division multiplexing, or different positions can be used as the bubble recognition position 62, bubble removal position 63, or preprocessing position 64 respectively.

[0161] For example, bubble recognition position 62, bubble removal position 63, and pretreatment position 64 are all located in reagent loading device 80 and are implemented through time-division multiplexing loading position 801. Alternatively, bubble recognition position 62, bubble removal position 63, and pretreatment position 64 are all located in reagent device 30 and are implemented through time-division multiplexing placement position 301b.

[0162] For example, taking the multiplexing loading position 801 as an example, the loading position 801 in the first time period is used as the bubble recognition position 62, the loading position 801 in the second time period is used as the bubble removal position 63, and the loading position 801 in the third time period is used as the preprocessing position 64. The first time period, the second time period, and the third time period are three different time periods, that is, the start time of any time period is different from the end time of another time period.

[0163] It can also be understood that in the embodiments of this application, the bubble recognition mechanism 81a can be fixedly set corresponding to the bubble recognition position 62, or it can be displaced relative to the bubble recognition position 62 to move closer to or away from the bubble recognition position 62. Similarly, the bubble removal device 82 can be set corresponding to the bubble removal position 63, or it can be displaced relative to the bubble removal position 63 to move closer to or away from the bubble removal position 63. Similarly, the pretreatment device 83 can be fixedly set corresponding to the pretreatment position 64, or it can be displaced relative to the pretreatment position 64 to move closer to or away from the pretreatment position 64.

[0164] This application also provides a method for processing reagent bubbles. This method can be applied to the aforementioned sample analyzer 100 and is executed by the processor 701 in the control device 70 of the sample analyzer 100. For example, the sample analyzer 100's corresponding program or instructions are stored in the memory 702. The processor 701 executes the method steps corresponding to the reagent bubble processing method provided in this application embodiment by calling the program stored in the memory 702.

[0165] It should be noted that, for the sake of convenience and brevity, the steps for handling reagent bubbles described below can be referred to the working process of control device 70 above, and will not be repeated here.

[0166] Please see Figure 23 , Figure 23 This is a schematic flowchart of a reagent bubble treatment method provided in an embodiment of this application.

[0167] like Figure 23 As shown, the method for processing reagent bubbles includes steps S101 to S103.

[0168] Step S101: Before the dispensing device of the sample analyzer draws the reagent from the reagent container, control the bubble recognition mechanism of the sample analyzer to perform a bubble recognition operation on the reagent container, and receive the bubble recognition information output by the bubble recognition mechanism. Step S102: Control the sample analyzer to perform bubble pretreatment on the reagent container according to the bubble recognition information. The bubble pretreatment includes at least one of the following: bubble removal operation and bubble avoidance operation. Step S103: Control the detection device of the sample analyzer to detect the target reaction liquid and obtain the corresponding detection data. The target reaction liquid is formed by mixing the reagent in the reagent container after bubble pretreatment with the sample to be tested.

[0169] In some embodiments, the method further includes, prior to pre-treating the bubbles within the reagent container: The bubble recognition information is analyzed to obtain the corresponding bubble recognition result. Based on the bubble recognition result, it is determined whether to perform an unloading operation or bubble pretreatment on the reagent container. The unloading operation is used to schedule the reagent container to a preset unloading position.

[0170] In some implementations, the bubble identification results include at least one of the following: height information of the bubble layer inside the reagent container, morphological information of the bubbles inside the reagent container, and distribution information of the bubbles inside the reagent container.

[0171] In some embodiments, the bubble identification result includes at least the height information of the bubble layer within the reagent container, the sample analyzer further includes a scheduling device, and the method further includes: If the height of the bubble layer inside the reagent container is confirmed to be greater than the first height threshold based on the height information, it is determined to perform an unloading operation on the reagent container. If the height of the bubble layer inside the reagent container is confirmed to be greater than the second height threshold and less than or equal to the first height threshold based on the height information, it is determined that a bubble removal operation should be performed on the reagent container, where the first height threshold is greater than the second height threshold.

[0172] In some embodiments, the method further includes: if it is confirmed based on height information that the height of the bubble layer inside the reagent container is less than or equal to a second height threshold, controlling the scheduling device to schedule the reagent container to the target placement position of the reagent device.

[0173] In some embodiments, the sample analyzer further includes an information prompting device, and the method further includes: After the reagent container is moved to the unloading position, the control information prompting device outputs the corresponding prompt information.

[0174] In some embodiments, the sample analyzer is provided with a bubble removal station, and the method further includes: If it is determined that a bubble removal operation needs to be performed on the reagent container based on the bubble recognition information, the control scheduling device will schedule the reagent container to the bubble removal position; The bubble removal device of the sample analyzer performs bubble removal operation on the reagent container located at the bubble removal position.

[0175] In some embodiments, the bubble removal device includes an ultrasonic device, and controlling the bubble removal device of the sample analyzer to perform a bubble removal operation on a reagent container located at the bubble removal position includes: controlling the ultrasonic device to output ultrasonic waves to the reagent container located at the bubble removal position to remove at least a portion of the bubbles in the reagent container. Alternatively, the bubble removal device includes a pressure regulating component that controls the bubble removal device of the sample analyzer to perform a bubble removal operation on a reagent container located at the bubble removal position, including: controlling the pressure regulating component to change the pressure inside the reagent container to remove at least a portion of the bubbles inside the reagent container; Alternatively, the bubble removal device includes a dispensing device that controls the bubble removal device of the sample analyzer to perform a bubble removal operation on a reagent container located at a bubble removal position, including: controlling the dispensing device to perform a bubble removal operation on the reagent container located at a bubble removal position to remove at least a portion of the bubbles in the reagent container.

[0176] In some embodiments, controlling the dispensing device to perform a bubble removal operation on the reagent container located at the bubble removal position includes: Based on the bubble recognition results, the dispensing device is controlled to perform a liquid aspiration operation on the reagent container located at the bubble removal position to remove at least some of the bubbles in the reagent container; After the liquid aspiration operation is completed, the dispensing device is moved to the drain position and the drain operation is performed.

[0177] In some embodiments, the dispensing device is controlled to perform a liquid aspiration operation on the reagent container located at the bubble removal position based on the bubble recognition result, including: A liquid aspiration control strategy is determined based on the bubble recognition result and a preset first association relationship. The first association relationship records the bubble recognition result and the liquid aspiration control strategy associated with the bubble recognition result. The liquid aspiration control strategy controls the dispensing device to perform a liquid aspiration operation on the reagent container located at the bubble removal position. The liquid aspiration control strategy includes at least one of the following: the target number of liquid aspiration operations performed by the dispensing device, the single liquid aspiration volume corresponding to each liquid aspiration operation performed by the dispensing device, the total liquid aspiration volume corresponding to the liquid aspiration operation performed by the dispensing device, the liquid aspiration speed of the pipette during the liquid aspiration operation, and the liquid aspiration height of the pipette during the liquid aspiration operation.

[0178] In some embodiments, the aspiration control strategy includes the aspiration height of the pipette needle during the aspiration operation of the dispensing device; the dispensing device includes a pipette needle, a needle movement mechanism, and a power mechanism, wherein the needle movement mechanism is used to drive the pipette needle to move; the power mechanism is used to provide power for the pipette needle to aspirate and dispense the target liquid, the target liquid including at least a reagent; controlling the dispensing device to perform an aspiration operation on the reagent container located at the bubble removal position according to the aspiration control strategy includes: The liquid level of the reagent in the reagent container is determined based on the bubble recognition results; The control needle moving mechanism moves the pipette to the first preset position inside the reagent container according to the aspiration height and liquid level height, so that the distance between the tip of the pipette and the reagent liquid surface matches the aspiration height; After the pipette moves to the first preset position, or during the process of the pipette moving to the preset position, the control power mechanism drives the pipette to perform the liquid aspiration action.

[0179] In some embodiments, the aspiration control strategy includes a target number of aspiration operations performed by the dispensing device; the dispensing device includes a pipette, a needle movement mechanism, and a power mechanism, wherein the needle movement mechanism drives the pipette to move; the power mechanism provides power for the pipette to aspirate and dispense the target liquid, the target liquid including at least a reagent; controlling the dispensing device to perform aspiration operations on the reagent container located at the bubble removal position according to the aspiration control strategy includes: The dispensing device is controlled to perform a target number of aspiration operations on the reagent container, wherein the aspiration operations include: The control needle moving mechanism moves the pipette to the preset aspiration position in the reagent container, and controls the power mechanism to drive the pipette to perform the aspiration action; After the aspiration action is completed, the control needle moving mechanism moves the pipette to the preset drainage position and controls the power mechanism to drive the pipette to perform the drainage action.

[0180] In some implementations, the target number of times is N, and N≥2. During the N aspiration operations, the distance between the tip of the pipette and the surface of the reagent liquid in the reagent container is the same each time the pipette performs the aspiration action; or, during the N aspiration operations, the distance between the tip of the pipette and the surface of the reagent liquid in the reagent container is a first distance when the pipette performs the aspiration action for the (N-1)th time, and a second distance when the pipette performs the aspiration action for the Nth time, wherein the first distance is greater than the second distance.

[0181] In some implementations, the target number of times is N, and N≥2, wherein during the N aspiration operations, the aspiration volume corresponding to the Nth aspiration action of the pipette is less than the aspiration volume corresponding to the (N-1)th aspiration action; and / or, the aspiration speed corresponding to the Nth aspiration action of the pipette is less than the aspiration speed corresponding to the (N-1)th aspiration action.

[0182] In some embodiments, the bubble layer within the reagent container includes a first type of bubble layer and a second type of bubble layer. The first type of bubble layer is located above the reagent liquid surface in the reagent container, and the second type of bubble layer is located on the side of the first type of bubble layer away from the reagent liquid surface. The bubble density of the second type of bubble layer is less than that of the first type of bubble layer. The aspiration control strategy includes the aspiration speed of the pipette needle during the aspiration operation performed by the dispensing device. Controlling the dispensing device to perform an aspiration operation on the reagent container located at the bubble removal position according to the aspiration control strategy includes: The dispensing device is controlled to perform at least one first aspiration operation on the second type of bubble layer, and the aspiration speed of the pipette needle during the first aspiration operation is the first aspiration speed. After the first liquid aspiration operation is completed, the dispensing device is controlled to perform a second liquid aspiration operation on the second type of bubble layer. During the second liquid aspiration operation, the liquid aspiration speed of the pipette needle is the second liquid aspiration speed, wherein the first liquid aspiration speed is greater than the second liquid aspiration speed.

[0183] In some embodiments, the sample analyzer is equipped with a bubble detection position, and the bubble detection result includes morphological information of bubbles in the reagent container and / or distribution information of bubbles in the reagent container. The sample analyzer also includes a scheduling device, and bubble pretreatment includes at least a bubble avoidance operation. The method further includes: If it is determined from the bubble recognition result that a bubble avoidance operation can be performed on the reagent container, the control scheduling device will schedule the reagent container located at the bubble recognition position to the target placement position of the reagent device. Based on the bubble recognition results, the dispensing device is controlled to avoid at least some of the bubbles in the reagent container located at the target placement position, and a liquid aspiration operation is performed on the reagent in the reagent container. The dispensing device controls the dispensing of the aspirated reagent into the reaction container so that the reagent and sample in the reagent container are mixed to form the target reaction solution, and controls the detection device to detect the target reaction solution to obtain the corresponding detection data.

[0184] In some embodiments, the method further includes: determining, based on morphological information and / or distribution information, whether the area in the reagent container where no air bubbles exist is located directly below the opening of the reagent container; if the area in the reagent container where no air bubbles exist is located directly below the opening of the reagent container, then it is determined that an air bubble avoidance operation can be performed on the reagent container.

[0185] In some embodiments, the bubble recognition mechanism includes an image acquisition mechanism, and controlling the bubble recognition mechanism to perform bubble recognition operations on the reagent container includes: The image acquisition mechanism is controlled to acquire images of the reagents stored in the storage chamber, and the reagent image data acquired by the image acquisition mechanism is used as bubble recognition information.

[0186] In some embodiments, the sample analyzer is equipped with a bubble detection position and a scheduling device. The image acquisition mechanism includes a light source component for emitting light signals and a signal acquisition component for acquiring light signals. The light source component and the signal acquisition component are respectively disposed on opposite sides of the bubble detection position in the horizontal direction. The method further includes: The control and scheduling device directs the reagent container to the bubble detection position; The light source assembly is controlled to emit a first light signal toward the reagent container located at the bubble recognition position; It also receives reagent image data output by the signal acquisition component based on the acquired second optical signal, wherein the second optical signal includes the optical signal formed by the first optical signal emitted through the reagent container.

[0187] In some embodiments, the sample analyzer is equipped with a bubble recognition position and a scheduling device. The image acquisition mechanism includes a light source component for emitting light signals and a signal acquisition component for acquiring light signals. The signal acquisition component and the light source component are positioned on the same side of the reagent container corresponding to the opening of the reagent container, or the signal acquisition component is positioned corresponding to the opening of the reagent container, and the light source component is positioned on the side of the reagent container adjacent to the opening. The method further includes: The control and scheduling device directs the reagent container to the bubble detection position; The light source assembly is controlled to emit a first light signal toward the reagent container located at the bubble recognition position; It also receives reagent image data output by the signal acquisition component based on the acquired second light signal, wherein the second light signal includes the reflected light signal of the first light signal reflected by the liquid surface of the reagent container.

[0188] In some embodiments, the dispensing device includes a pipette and a needle movement mechanism. The electrical properties of the pipette may change when it comes into contact with the target analyte, which includes at least air bubbles. The bubble recognition mechanism includes a pipette, and the sample analyzer is equipped with a bubble recognition position and a scheduling device. The method further includes: The control and scheduling device directs the reagent container to the bubble detection position; The control needle moving mechanism drives the pipette from a preset position to the reagent container located at the bubble detection position, and during the movement of the pipette, it acquires the bubble recognition information generated by the bubble recognition mechanism based on the detected electrical information of the pipette.

[0189] In some embodiments, the sample analyzer further includes a reagent loading device and a scheduling device, and the sample analyzer is also provided with a bubble recognition position and a bubble removal position. The bubble pretreatment includes at least a bubble removal operation, and the method further includes: The control and scheduling device schedules the reagent container located in the reagent loading device to the bubble recognition position, and controls the bubble recognition mechanism to perform bubble recognition operation on the reagent container located in the bubble recognition position to output the corresponding bubble recognition information; If it is determined from the bubble recognition information that bubble pretreatment is required in the reagent container, the control scheduling device will schedule the reagent container to the bubble removal position and control the bubble removal device of the sample analyzer to perform bubble removal operation on the reagent container.

[0190] In some embodiments, the bubble recognition mechanism is disposed on the reagent loading device or on the reagent loading port of the reagent loading device, wherein the bubble recognition position is disposed corresponding to the bubble recognition mechanism and is located on the reagent loading device; Alternatively, the bubble recognition mechanism is located in the reagent device, and the bubble recognition position is located in the reagent device corresponding to the bubble recognition mechanism. Alternatively, the bubble recognition mechanism is located on the reagent container scheduling path between the reagent loading device and the reagent device, and the bubble recognition position corresponds to the setting of the bubble recognition mechanism and is located on the reagent container scheduling path.

[0191] In some embodiments, the reagent container includes a container body and a cap, wherein the container body forms a storage cavity with an opening for storing reagents, and the cap closes to the opening of the container body to seal the opening. The sample analyzer further includes a pretreatment device for releasing the sealing fit between the container body and the cap, so that the dispensing device can draw reagents from the reagent container; the method further includes: Before the bubble recognition mechanism performs bubble recognition on the reagents stored in the storage chamber, the pretreatment device pretreatment the reagent container to at least remove the sealing fit between the container body and the lid.

[0192] It should be understood that the terminology used in this application specification is for the purpose of describing particular embodiments only and is not intended to limit the application. As used in this application specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to include the plural forms unless the context clearly indicates otherwise.

[0193] It should also be understood that the term "and / or" as used in this application specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that in this application, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or system that includes that element.

[0194] The sequence numbers of the embodiments in this application are for descriptive purposes only and do not represent the superiority or inferiority of the embodiments. The above descriptions are merely specific implementations of this application, but the scope of protection of this application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in this application, and these modifications or substitutions should all be covered within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A sample analyzer, characterized in that, include: A reagent device is provided with at least one placement position for placing a reagent container for storing reagents; The dispensing device is at least used to draw up the reagent from the reagent container and discharge it into the reaction container so that the sample and reagent are mixed in the reaction container to form a reaction solution. A detection device is used to detect the reaction solution in order to obtain corresponding detection data; A bubble recognition mechanism is used to perform bubble recognition operations on the reagent container and output corresponding bubble recognition information; Control device, at least for: Before the dispensing device draws the reagent from the reagent container, the bubble recognition mechanism is controlled to perform a bubble recognition operation on the reagent container, and the bubble recognition information output by the bubble recognition mechanism during the bubble recognition operation is received. Based on the bubble recognition information, the sample analyzer is controlled to perform bubble pretreatment on the reagent container. The bubble pretreatment includes at least one of bubble removal operation and bubble avoidance operation. The detection device is controlled to detect the target reaction solution and obtain corresponding detection data, wherein the target reaction solution is formed by mixing the reagent and sample in the reagent container after the bubble pretreatment.

2. The sample analyzer according to claim 1, characterized in that, Before performing bubble pretreatment on the bubbles in the reagent container, the control device is also used to: The bubble recognition information is analyzed to obtain the corresponding bubble recognition result. Based on the bubble recognition result, it is determined whether to perform an unloading operation on the reagent container or to perform bubble pretreatment. The unloading operation is used to schedule the reagent container to a preset unloading position.

3. The sample analyzer according to claim 2, characterized in that, The bubble identification result includes at least one of the following: the height information of the bubble layer inside the reagent container, the morphological information of the bubbles inside the reagent container, and the distribution information of the bubbles inside the reagent container.

4. The sample analyzer according to claim 3, characterized in that, The bubble identification result includes at least the height information of the bubble layer inside the reagent container. The sample analyzer also includes a scheduling device, and the control device is further used for: If the height of the bubble layer inside the reagent container is confirmed to be greater than a first height threshold based on the height information, it is determined to perform an unloading operation on the reagent container. If, based on the height information, it is confirmed that the height of the bubble layer inside the reagent container is greater than a second height threshold and less than or equal to the first height threshold, it is determined that the bubble removal operation will be performed on the reagent container, wherein the first height threshold is greater than the second height threshold.

5. The sample analyzer according to claim 4, characterized in that, The control device is further configured to: control the scheduling device to schedule the reagent container to the target placement position of the reagent device when it is confirmed, based on the height information, that the height of the bubble layer inside the reagent container is less than or equal to the second height threshold.

6. The sample analyzer according to claim 4, characterized in that, The sample analyzer also includes an information prompting device, and the control device is further used for: After the reagent container is moved to the unloading position, the information prompting device is controlled to output the corresponding prompt information.

7. The sample analyzer according to claim 4, characterized in that, The sample analyzer is equipped with a bubble removal station, and the control device is also used for: If it is determined from the bubble recognition information that the bubble removal operation needs to be performed on the reagent container, the scheduling device is controlled to schedule the reagent container to the bubble removal position. The bubble removal device of the sample analyzer is controlled to perform a bubble removal operation on the reagent container located at the bubble removal position.

8. The sample analyzer according to claim 7, characterized in that, The bubble removal device includes an ultrasonic device. The control device controls the bubble removal device of the sample analyzer to perform a bubble removal operation on the reagent container located at the bubble removal position, including: controlling the ultrasonic device to output ultrasonic waves to the reagent container located at the bubble removal position to remove at least a portion of the bubbles in the reagent container. Alternatively, the bubble removal device includes a pressure regulating component, and the control device controls the bubble removal device of the sample analyzer to perform a bubble removal operation on the reagent container located at the bubble removal position, including: controlling the pressure regulating component to change the pressure inside the reagent container to remove at least a portion of the bubbles inside the reagent container; Alternatively, the bubble removal device includes the dispensing device, and the control device controls the bubble removal device of the sample analyzer to perform a bubble removal operation on the reagent container located at the bubble removal position, including: controlling the dispensing device to perform a bubble removal operation on the reagent container located at the bubble removal position to remove at least a portion of the bubbles in the reagent container.

9. The sample analyzer according to claim 8, characterized in that, The control device controls the dispensing device to perform a bubble removal operation on the reagent container located at the bubble removal position, including: Based on the bubble recognition result, the dispensing device is controlled to perform a liquid aspiration operation on the reagent container located at the bubble removal position to remove at least a portion of the bubbles in the reagent container; After the liquid aspiration operation is completed, the dispensing device is controlled to move to the drain position and perform the drain operation.

10. The sample analyzer according to claim 9, characterized in that, The step of controlling the dispensing device to perform a liquid aspiration operation on the reagent container located at the bubble removal position based on the bubble recognition result includes: A liquid aspiration control strategy is determined based on the bubble recognition result and a preset first association relationship. The first association relationship records the bubble recognition result and the liquid aspiration control strategy associated with the bubble recognition result. The liquid aspiration control strategy controls the dispensing device to perform a liquid aspiration operation on the reagent container located at the bubble removal position. The liquid aspiration control strategy includes at least one of the following: the target number of liquid aspiration operations performed by the dispensing device, the single liquid aspiration volume corresponding to each liquid aspiration operation performed by the dispensing device, the total liquid aspiration volume corresponding to each liquid aspiration operation performed by the dispensing device, the liquid aspiration speed of the pipette during the liquid aspiration operation performed by the dispensing device, and the liquid aspiration height of the pipette during the liquid aspiration operation performed by the dispensing device.

11. The sample analyzer according to claim 10, characterized in that, The liquid aspiration control strategy includes the aspiration height of the pipette needle during the liquid aspiration operation of the dispensing device; the dispensing device includes a pipette needle, a needle moving mechanism, and a power mechanism, wherein the needle moving mechanism is used to drive the pipette needle to move; the power mechanism is used to provide power for the pipette needle to aspirate and dissipate the target liquid, wherein the target liquid includes at least the reagent; controlling the dispensing device to perform a liquid aspiration operation on the reagent container located at the bubble removal position according to the liquid aspiration control strategy includes: The liquid level of the reagent in the reagent container is determined based on the bubble recognition results; The needle moving mechanism is controlled according to the aspiration height and the liquid level height to move the pipette to a first preset position inside the reagent container, so that the distance between the tip of the pipette and the reagent liquid surface matches the aspiration height; After the pipette moves to the first preset position, or during the process of the pipette moving to the preset position, the power mechanism is controlled to drive the pipette to perform a liquid aspiration action.

12. The sample analyzer according to claim 10, characterized in that, The liquid aspiration control strategy includes a target number of times the dispensing device performs liquid aspiration operations; the dispensing device includes a pipette, a needle moving mechanism, and a power mechanism, wherein the needle moving mechanism is used to drive the pipette to move; the power mechanism is used to provide power for the pipette to aspirate and dissipate the target liquid, the target liquid including at least the reagent; controlling the dispensing device to perform liquid aspiration operations on the reagent container located at the bubble removal position according to the liquid aspiration control strategy includes: The dispensing device is controlled to perform the target number of aspiration operations on the reagent container, wherein the aspiration operations include: The needle moving mechanism is controlled to move the pipette to the preset aspiration position in the reagent container, and the power mechanism is controlled to drive the pipette to perform the aspiration action; After the aspiration action is completed, the needle moving mechanism is controlled to move the pipette to the preset drainage position and the power mechanism is controlled to drive the pipette to perform the drainage action.

13. The sample analyzer according to claim 12, characterized in that, The target number of times is N, and N≥2. During the N liquid aspiration operations, the distance between the tip of the pipette and the surface of the reagent liquid in the reagent container is the same each time the pipette performs the liquid aspiration action; or, during the N liquid aspiration operations, the distance between the tip of the pipette and the surface of the reagent liquid in the reagent container is a first distance when the pipette performs the liquid aspiration action for the (N-1)th time, and a second distance when the pipette performs the liquid aspiration action for the Nth time, wherein the first distance is greater than the second distance.

14. The sample analyzer according to claim 12, characterized in that, The target number of times is N, and N≥2, wherein, during the N aspiration operations, the aspiration volume corresponding to the Nth aspiration action of the pipette is less than the aspiration volume corresponding to the (N-1)th aspiration action; and / or, the aspiration speed corresponding to the Nth aspiration action of the pipette is less than the aspiration speed corresponding to the (N-1)th aspiration action.

15. The sample analyzer according to claim 12, characterized in that, The bubble layer within the reagent container includes a first type of bubble layer and a second type of bubble layer. The first type of bubble layer is located above the reagent liquid surface in the reagent container, and the second type of bubble layer is located on the side of the first type of bubble layer away from the reagent liquid surface. The bubble density of the second type of bubble layer is less than that of the first type of bubble layer. The aspiration control strategy includes the aspiration speed of the pipette needle during the aspiration operation performed by the dispensing device. Controlling the dispensing device to perform an aspiration operation on the reagent container located at the bubble removal position according to the aspiration control strategy includes: The dispensing device is controlled to perform at least one first liquid aspiration operation on the second type of bubble layer, and the liquid aspiration speed of the pipette needle during the first liquid aspiration operation is the first liquid aspiration speed; After the first liquid aspiration operation is completed, the dispensing device is controlled to perform a second liquid aspiration operation on the second type of bubble layer, and the liquid aspiration speed of the pipette needle during the second liquid aspiration operation is the second liquid aspiration speed, wherein the first liquid aspiration speed is greater than the second liquid aspiration speed.

16. The sample analyzer according to claim 3, characterized in that, The sample analyzer is equipped with a bubble recognition position. The bubble recognition result includes the morphological information of bubbles in the reagent container and / or the distribution information of bubbles in the reagent container. The sample analyzer also includes a scheduling device. Bubble pretreatment includes at least the bubble avoidance operation. The control device is further used for: If it is determined from the bubble recognition result that the bubble avoidance operation can be performed on the reagent container, the scheduling device is controlled to schedule the reagent container located at the bubble recognition position to the target placement position of the reagent device; Based on the bubble recognition result, the dispensing device is controlled to avoid at least some of the bubbles in the reagent container located at the target placement position, and a liquid aspiration operation is performed on the reagent in the reagent container; The dispensing device is controlled to discharge the aspirated reagent into the reaction container so that the reagent and sample in the reagent container are mixed to form the target reaction solution, and the detection device is controlled to detect the target reaction solution to obtain the corresponding detection data.

17. The sample analyzer according to claim 16, characterized in that, The control device is further configured to: determine, based on the morphological information and / or the distribution information, whether the area in the reagent container where no air bubbles exist is located directly below the opening of the reagent container; if the area in the reagent container where no air bubbles exist is located directly below the opening of the reagent container, then determine that the air bubble avoidance operation can be performed on the reagent container.

18. The sample analyzer according to claim 1, characterized in that, The bubble recognition mechanism includes an image acquisition mechanism, and the control device controls the bubble recognition mechanism to perform a bubble recognition operation on the reagent container, including: The image acquisition mechanism is controlled to acquire images of the reagents stored in the storage cavity of the reagent container, and the reagent image data acquired by the image acquisition mechanism is used as the bubble recognition information.

19. The sample analyzer according to claim 18, characterized in that, The sample analyzer is equipped with a bubble detection position and a scheduling device. The image acquisition mechanism includes a light source component for emitting light signals and a signal acquisition component for acquiring light signals. The light source component and the signal acquisition component are respectively arranged on opposite sides of the bubble detection position in the horizontal direction. The control device is further used for: The scheduling device is controlled to schedule the reagent container to the bubble recognition position; The light source assembly is controlled to emit a first light signal toward the reagent container located at the bubble recognition position; The system receives reagent image data output by the signal acquisition component based on the acquired second optical signal, wherein the second optical signal includes the optical signal formed by the first optical signal emitted through the reagent container.

20. The sample analyzer according to claim 18, characterized in that, The sample analyzer is equipped with a bubble recognition position and a scheduling device. The image acquisition mechanism includes a light source component for emitting light signals and a signal acquisition component for acquiring light signals. The signal acquisition component and the light source component are positioned on the same side of the reagent container corresponding to the opening of the reagent container, or the signal acquisition component is positioned corresponding to the opening of the reagent container, and the light source component is positioned on the side of the reagent container adjacent to the opening. The control device is further used for: The scheduling device is controlled to schedule the reagent container to the bubble recognition position; The light source assembly is controlled to emit a first light signal toward the reagent container located at the bubble recognition position; The system receives reagent image data output by the signal acquisition component based on the acquired second optical signal, wherein the second optical signal includes the reflected light signal of the first optical signal reflected by the liquid surface of the reagent container.

21. The sample analyzer according to claim 1, characterized in that, The dispensing device includes a pipette and a pipette movement mechanism. The electrical properties of the pipette may change when it comes into contact with the target object. The target object includes at least air bubbles. The bubble recognition mechanism includes the pipette. The sample analyzer is equipped with a bubble recognition position and a scheduling device. The control device is also used for: The scheduling device is controlled to schedule the reagent container to the bubble recognition position; The needle moving mechanism is controlled to drive the pipette from a preset position to the reagent container located at the bubble recognition position, and during the movement of the pipette, the bubble recognition information generated by the bubble recognition mechanism based on the detected electrical information of the pipette is acquired.

22. The sample analyzer according to claim 1, characterized in that, The sample analyzer also includes a reagent loading device and a scheduling device, and is further equipped with a bubble recognition position and a bubble removal position. The bubble pretreatment includes at least the bubble removal operation. The control device is also used for: The scheduling device is controlled to schedule the reagent container located in the reagent loading device to the bubble recognition position, and the bubble recognition mechanism is controlled to perform bubble recognition operation on the reagent container located in the bubble recognition position to output the corresponding bubble recognition information; If it is determined from the bubble identification information that bubble pretreatment is required in the reagent container, the scheduling device is controlled to schedule the reagent container to the bubble removal position, and the bubble removal device of the sample analyzer is controlled to perform bubble removal operation on the reagent container.

23. The sample analyzer according to claim 22, characterized in that, The bubble recognition mechanism is disposed on the reagent loading device or on the reagent loading port of the reagent loading device, wherein the bubble recognition position is disposed corresponding to the bubble recognition mechanism and is located on the reagent loading device; Alternatively, the bubble recognition mechanism is disposed on the reagent device, and the bubble recognition position is disposed corresponding to the bubble recognition mechanism and located on the reagent device; Alternatively, the bubble recognition mechanism is located on the reagent container scheduling path between the reagent loading device and the reagent device, and the bubble recognition position is located corresponding to the bubble recognition mechanism and on the reagent container scheduling path.

24. The sample analyzer according to claim 1, characterized in that, The reagent container includes a container body and a cap, wherein the container body forms a storage cavity with an opening for storing reagents, and the cap closes to the opening of the container body to seal the opening. The sample analyzer further includes a pretreatment device for releasing the sealing fit between the container body and the cap, so that the dispensing device can draw reagents from the reagent container. The control device is further used for: Before controlling the bubble recognition mechanism to perform bubble recognition on the reagents stored in the storage cavity, the pretreatment device is controlled to pretreatment the reagent container to at least release the sealing fit between the container body and the lid.

25. A method for treating reagent bubbles, characterized in that, Applied to a sample analyzer, the method includes: Before the dispensing device of the sample analyzer draws the reagent from the reagent container, the bubble recognition mechanism of the sample analyzer is controlled to perform a bubble recognition operation on the reagent container, and the bubble recognition information output by the bubble recognition mechanism is received. Based on the bubble recognition information, the sample analyzer is controlled to perform bubble pretreatment on the reagent container. The bubble pretreatment includes at least one of bubble removal operation and bubble avoidance operation. The detection device of the sample analyzer is controlled to detect the target reaction solution and obtain corresponding detection data. The target reaction solution is formed by mixing the reagent in the reagent container after the bubble pretreatment with the sample to be tested.

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