A coffee composition for alleviating anxiety and a method of preparing the same
A coffee composition was prepared by combining coffee beans, Eucommia ulmoides extract, and oyster polypeptide powder, which solved the problem of the lack of effective anxiety relief in the existing technology and achieved a safe and effective anxiety regulation effect.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INST OF SCI & TECHNICAL INFORMATIONCATAS
- Filing Date
- 2026-03-23
- Publication Date
- 2026-07-03
AI Technical Summary
There is a lack of effective coffee compositions in the current technology for relieving anxiety, and existing drug treatments are highly addictive or psychotherapy is slow to take effect, making it difficult to meet the conditioning needs of large populations and subclinical anxiety states.
A coffee composition was prepared using a combination of coffee beans, Eucommia ulmoides extract, oyster polypeptide powder, xylitol, and sorbitol through a specific process. The Eucommia ulmoides extract and oyster polypeptide powder were used to inhibit the overactivation of the hypothalamus-pituitary-adrenal axis, reduce abnormal cortisol secretion, and regulate the calcium homeostasis of nerve cells.
It addresses the root cause of anxiety symptoms, reduces abnormal cortisol secretion, and alleviates chronic anxiety-related symptoms such as palpitations and hand tremors. It is also non-addictive and highly safe.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of coffee product technology, specifically relating to a coffee composition for relieving anxiety and its preparation method. Background Technology
[0002] With the accelerating pace of society, increasing life pressures, and a rise in sudden stressful events, anxiety disorders have become one of the most common mental disorders. Anxiety disorders not only severely reduce patients' quality of life, work efficiency, and social skills, but may also trigger comorbidities such as depression, cardiovascular disease, and immune system disorders, imposing a heavy medical and economic burden on individuals, families, and society.
[0003] Current methods for relieving anxiety mainly include medication and psychotherapy. While SSRIs and benzodiazepines can quickly relieve symptoms, they are highly addictive. Psychotherapy, on the other hand, is limited by available resources and has a slow onset of action, making it difficult to meet the needs of large populations and those with subclinical anxiety. Currently, traditional Chinese medicine treatments are gaining popularity. For example, Chinese patent CN 112546120 A describes a folic acid-containing anxiety-relieving product that combines folic acid compounds, ginkgo biloba, goji berries, and lotus seeds. This product offers significant anti-anxiety effects, has no toxic side effects, and tastes good, making it ideal as a snack for modern people under high stress and frequently experiencing anxiety. It can effectively relieve anxiety and benefit physical and mental health. Another example is Chinese patent CN 104547906. A traditional Chinese medicine composition and its application for relieving anxiety and tension is disclosed. It comprises a mixture of raw materials in the following weight ratios: Schisandra chinensis 100-300 parts, Ophiopogon japonicus 200-500 parts, Rubus idaeus 150-450 parts, Atractylodes macrocephala 200-500 parts, Bambusa textilis 150-450 parts, roasted Ziziphus jujuba var. spinosa 100-300 parts, and Curcuma longa 100-300 parts. This composition demonstrates safety and efficacy in relieving anxiety and tension associated with polycystic ovary syndrome. The composition contains few ingredients, is easy to control in terms of quality, and is inexpensive. However, there are currently no reports or studies on coffee compositions used to relieve anxiety. Summary of the Invention
[0004] The purpose of this invention is to provide a coffee composition for relieving anxiety and a method for preparing the same, which can be used to relieve anxiety.
[0005] This invention provides a coffee composition for relieving anxiety, comprising, by weight parts, the following ingredients: 15-20 parts coffee beans, 10-14 parts Eucommia ulmoides extract, 5-12 parts oyster polypeptide powder, 6-10 parts xylitol and 3-5 parts sorbitol.
[0006] Preferably, the method for preparing the oyster polypeptide powder includes: Oyster meat, flavor protease, and water were mixed and enzymatically hydrolyzed. After enzyme inactivation and solid-liquid separation, a filtrate was obtained. The filtrate was then concentrated to obtain oyster polypeptide powder.
[0007] Preferably, during mixed enzymatic hydrolysis, the mass ratio of oyster meat, flavor protease, and water is 10-15:5-8:85-90; The temperature for mixed enzymatic hydrolysis is 48-55℃, and the time is 8-10 hours.
[0008] Preferably, the method for preparing the Eucommia ulmoides extract includes: Eucommia ulmoides was extracted with supercritical CO2 with the aid of an entrainer to obtain Eucommia ulmoides residue. The residue was then extracted with an acidic solution and filtered to obtain an acid extract and the extracted residue. The extracted residue was then mixed with water, cooked, and filtered to obtain an aqueous extract and the cooked residue. The acid extract and aqueous extract were combined, and the mixture was subjected to activated carbon adsorption, ethanol recovery, and spray drying to obtain Eucommia ulmoides extract.
[0009] The acidic solution includes ethanol and citric acid.
[0010] Preferably, the entrainer comprises a methanol solution; the volume concentration of the methanol solution is 0.5%-2%.
[0011] Preferably, the supercritical CO2 extraction is performed at a pressure of 20-30 MPa, a temperature of 45-55°C, and a time of 15-20 min.
[0012] Preferably, during extraction, the volume-to-mass ratio of the acidic solution to the Eucommia ulmoides residue is 10-12 mL: 1 g; The extraction temperature is 35-40℃, and the time is 5-6 hours; The acidic solution uses water as a solvent and includes: ethanol with a volume concentration of 45%-55% and citric acid with a mass concentration of 0.5%-1%.
[0013] Preferably, during the steaming process, the volume-to-mass ratio of water to the extracted Eucommia ulmoides residue is 10-12 mL: 1 g.
[0014] The extraction temperature is 80-95℃ and the time is 40-60 minutes.
[0015] This invention provides a method for preparing the coffee composition described above, comprising the following steps: Coffee beans are roasted and ground to obtain coffee powder.
[0016] Coffee powder, Eucommia ulmoides extract, oyster polypeptide powder, xylitol and sorbitol are mixed evenly to obtain a coffee composition.
[0017] The present invention provides the use of the coffee composition described above in the preparation of health products or pharmaceuticals for relieving anxiety.
[0018] Beneficial effects: The present invention provides a coffee composition for relieving anxiety, comprising, by weight, the following ingredients: 15-20 parts coffee beans, 10-14 parts Eucommia ulmoides extract, 5-12 parts oyster polypeptide powder, 6-10 parts xylitol and 3-5 parts sorbitol.
[0019] This invention blends coffee beans, Eucommia ulmoides extract, oyster polypeptide powder, xylitol, and sorbitol. Eucommia ulmoides extract and oyster polypeptide, as food-grade and bioactive peptides, can inhibit overactivation of the hypothalamus-pituitary-adrenal (HPA) axis, reducing abnormal secretion of corticotropin-releasing hormone and cortisol, thus normalizing elevated cortisol levels associated with chronic anxiety. Furthermore, the active ingredients are easily absorbed by the body, providing nutrition to nerve cells, repairing nerve cell membranes, and regulating nerve cell calcium homeostasis, effectively alleviating symptoms such as palpitations and tremors associated with anxiety. Therefore, this formula addresses both the root cause and symptoms, is non-addictive, highly safe, and suitable for relieving anxiety. Detailed Implementation
[0020] To further illustrate the present invention, the solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0021] The coffee beans used in this invention are Robusta coffee beans ( Coffea canephora Eucommia ulmoides ( Eucommia ulmoides Oliv.), oysters are long oysters ( Crassostrea gigas ).
[0022] Example 1 The steps for preparing a coffee composition for relieving anxiety are as follows: (1) Oyster polypeptide powder Clean the oyster meat, dry it, and then crush it to obtain crushed oyster meat; The crushed oyster meat, flavor protease and water were mixed evenly at a mass ratio of 10:8:90 and enzymatically hydrolyzed at 55℃ for 10 hours to obtain the enzymatic hydrolysate; the enzymatic hydrolysate was then subjected to enzyme inactivation and solid-liquid separation to obtain the filtrate. The filtrate was concentrated to obtain oyster polypeptide powder, which had a water content of 10%.
[0023] (2) Eucommia ulmoides extract Select Eucommia ulmoides leaves, wash them thoroughly, and set them aside. The acidic solution uses water as a solvent and contains 45% ethanol by volume and 0.5% citric acid by mass. Using a methanol solution (volume concentration of 2%) as an entrainer, supercritical CO2 extraction was performed on the leaves of Eucommia ulmoides (pressure 20 MPa, temperature 45℃, time 150 min) to obtain Eucommia ulmoides residue. The acidic solution and Eucommia ulmoides residue were mixed at a volume-to-mass ratio of 10 mL: 1 g and then extracted at 40 °C for 6 h with stirring during the extraction (stirring once per hour). After filtration, the acidic extract and the extracted Eucommia ulmoides residue were obtained. The eucommia residue after extraction was mixed with water at a mass-volume ratio of 1g:10mL, and then boiled at 80℃ for 60min. After filtration, the water extract and the boiled eucommia residue were obtained. After merging the acid extract and the water extract, a mixture was obtained. The mixture was then mixed with activated carbon at a mass ratio of 100:1 for thorough adsorption. After removing the activated carbon and recovering the ethanol, the mixture was spray-dried to obtain the Eucommia ulmoides extract.
[0024] (3) Coffee composition The coffee composition consists of the following ingredients by weight: 15 parts coffee beans, 10 parts Eucommia ulmoides extract, 5 parts oyster polypeptide powder, 6 parts xylitol and 4 parts sorbitol; Coffee beans are roasted and ground to obtain coffee powder; Coffee powder, Eucommia ulmoides extract, oyster polypeptide powder, xylitol and sorbitol are mixed evenly to obtain a coffee composition.
[0025] Example 2 The steps for preparing a coffee composition for relieving anxiety are as follows: (1) Oyster polypeptide powder Clean the oyster meat, dry it, and then crush it to obtain crushed oyster meat; The crushed oyster meat, flavor protease and water were mixed evenly at a mass ratio of 15:5:85 and enzymatically hydrolyzed at 55℃ for 10 hours to obtain the enzymatic hydrolysate; the enzymatic hydrolysate was then subjected to enzyme inactivation and solid-liquid separation to obtain the filtrate. The filtrate was concentrated to obtain oyster polypeptide powder, which had a water content of 10%.
[0026] (2) Eucommia ulmoides extract Select Eucommia ulmoides leaves, wash them thoroughly, and set them aside. The acidic solution uses water as a solvent and contains 45% ethanol by volume and 0.5% citric acid by mass. Using a methanol solution (volume concentration of 2%) as an entrainer, supercritical CO2 extraction was performed on the leaves of Eucommia ulmoides (pressure 20 MPa, temperature 45℃, time 150 min) to obtain Eucommia ulmoides residue. The acidic solution and Eucommia ulmoides residue were mixed at a volume-to-mass ratio of 12 mL: 1 g and then extracted at 40 °C for 6 h with stirring during the extraction (stirring once per hour). After filtration, the acidic extract and the extracted Eucommia ulmoides residue were obtained. The eucommia residue after extraction was mixed with water at a mass-volume ratio of 1g:12mL, and then boiled at 80℃ for 60min. After filtration, the water extract and the boiled eucommia residue were obtained. After merging the acid extract and the water extract, a mixture was obtained. The mixture was then mixed with activated carbon at a mass ratio of 100:1 for thorough adsorption. After removing the activated carbon and recovering the ethanol, the mixture was spray-dried to obtain the Eucommia ulmoides extract.
[0027] (3) Coffee composition The coffee composition consists of the following ingredients by weight: 20 parts coffee beans, 14 parts Eucommia ulmoides extract, 10 parts oyster polypeptide powder, 6 parts xylitol and 5 parts sorbitol; Coffee beans are roasted and ground to obtain coffee powder; Coffee powder, Eucommia ulmoides extract, oyster polypeptide powder, xylitol and sorbitol are mixed evenly to obtain a coffee composition.
[0028] Comparative Example 1 The only difference from Example 1 is that step (1) in Comparative Example 1 is as follows: (1) Oyster polypeptide powder Clean the oyster meat, dry it, and then crush it to obtain crushed oyster meat; The crushed oyster meat, papain, and water were mixed evenly at a mass ratio of 10:8:90 and enzymatically hydrolyzed at 55℃ for 10 hours to obtain the enzymatic hydrolysate. The enzymatic hydrolysate was then subjected to enzyme inactivation and solid-liquid separation to obtain the filtrate. The filtrate was concentrated to obtain oyster polypeptide powder, which had a water content of 10%.
[0029] Comparative Example 2 The only difference from Example 1 is that step (2) in Comparative Example 2 is as follows: (2) Eucommia ulmoides extract Select Eucommia ulmoides leaves, wash them thoroughly, and set them aside. The acidic solution uses water as a solvent and contains 45% ethanol by volume and 0.5% citric acid by mass. Eucommia ulmoides leaves were subjected to supercritical CO2 extraction (pressure 20 MPa, temperature 45 °C, time 150 min) using an ethanol solution (volume concentration of 2%) as an entrainer to obtain Eucommia ulmoides residue. The acidic solution and Eucommia ulmoides residue were mixed at a volume-to-mass ratio of 10 mL: 1 g and then extracted at 40 °C for 6 h with stirring during the extraction (stirring once per hour). After filtration, the acidic extract and the extracted Eucommia ulmoides residue were obtained. The eucommia residue after extraction was mixed with water at a mass-volume ratio of 1g:10mL, and then boiled at 80℃ for 60min. After filtration, the water extract and the boiled eucommia residue were obtained. After merging the acid extract and the water extract, a mixture was obtained. The mixture was then mixed with activated carbon at a mass ratio of 100:1 for thorough adsorption. After removing the activated carbon and recovering the ethanol, the mixture was spray-dried to obtain the Eucommia ulmoides extract.
[0030] Comparative Example 3 The only difference from Example 1 is that step (2) in Comparative Example 3 is as follows: (2) Eucommia ulmoides extract Select Eucommia ulmoides leaves, wash them thoroughly, and set them aside. The acidic solution uses water as a solvent and contains 45% ethanol by volume and 0.5% citric acid by mass. Using a methanol solution (volume concentration of 2%) as an entrainer, supercritical CO2 extraction was performed on the leaves of Eucommia ulmoides (pressure 20 MPa, temperature 45℃, time 150 min) to obtain Eucommia ulmoides residue. The acidic solution and Eucommia ulmoides residue were mixed at a volume-to-mass ratio of 10 mL: 1 g and then extracted at 40 °C for 6 h with stirring during the extraction (stirring once per hour). After filtration, the acidic extract and the extracted Eucommia ulmoides residue were obtained. The acid extract was mixed with activated carbon at a mass ratio of 100:1 for thorough adsorption. After removing the activated carbon and recovering the ethanol, the extract was obtained by spray drying.
[0031] Comparative Example 4 The only difference from Example 1 is that it uses Rosa laevigata ( Rosa laevigata Michx. Replace Eucommia ulmoides in Example 1.
[0032] Comparative Example 5 The only difference from Example 1 is that it uses Rosa laevigata ( Rosa laevigata Michx. The water extract replaced the oyster polypeptide powder in Example 1.
[0033] Comparative Example 6 The only difference from Example 1 is that step (3) in Comparative Example 6 is as follows: (3) Coffee composition The coffee composition consists of the following ingredients by weight: 15 parts coffee beans, 10 parts Eucommia ulmoides extract, 20 parts oyster polypeptide powder, 6 parts xylitol and 4 parts sorbitol; Coffee beans are roasted and ground to obtain coffee powder; Coffee powder, Eucommia ulmoides extract, oyster polypeptide powder, xylitol and sorbitol are mixed evenly to obtain a coffee composition.
[0034] Tail suspension test Several healthy male Kunming mice weighing 20-22g were raised at 20-24℃ and 50%-60% humidity for one week. After the mice showed no abnormalities, the experiment was conducted.
[0035] Mice were randomly divided into a model group, a positive control group, and an experimental group, with 10 mice in each group. The experimental group was fed 0.5 g / kg of the coffee composition from Examples 1 and 2, and Comparative Examples 1 through 6 (Experimental Group 1 corresponds to Example 1, Experimental Group 2 corresponds to Example 2, and so on, up to Experimental Group 8 corresponds to Comparative Example 6). The positive control group received fluoxetine hydrochloride (0.01 g / kg). Both the positive control and experimental groups were prepared as suspensions with physiological saline. The model group received an equal dose of physiological saline during the administration period. Each mouse in each group was administered the medication once daily by gavage for 14 consecutive days, and the test was performed 2 hours after the last administration.
[0036] Mouse tail suspension test: The tail of the mouse was attached to the support at the top of the tail suspension box, suspending the mouse upside down. The head of the mouse was 10 cm above the bottom of the box. Two mice were suspended at a time, separated by a partition to prevent collision. The mice struggled due to panic after being suspended upside down. When they became fatigued from struggling, they became disillusioned and intermittently immobile. The mice were suspended for 10 minutes, and the cumulative immobility time of the mice in the last 5 minutes was recorded. The results are shown in Table 1 (Table 1 shows the average immobility time of each mouse in each group).
[0037] Table 1 Mouse tail suspension test
[0038] As shown in Table 1, compared with the model group, the tail suspension immobility time of mice in both the positive group and the experimental group decreased. This indicates that the coffee compositions prepared in the examples and comparative examples can alleviate anxiety to varying degrees, but the coffee composition prepared in the examples is more effective in relieving anxiety.
[0039] Blood CORT level test Several healthy male Kunming mice weighing 20-22g were raised at 20-24℃ and 50%-60% humidity for one week. After the mice showed no abnormalities, the experiment was conducted.
[0040] A chronic restraint stress model was established in mice using a restraint device: a small vent hole was drilled into the bottom of a 50mL centrifuge tube, ensuring the hole's edge was rounded to avoid unnecessary injury to the mice and reduce experimental uncertainty. Mice were placed in the processed centrifuge tube at a fixed time each day for 6 hours of restraint stress. After placement, the tube cap was tightened, and the tube was placed horizontally and secured, maintaining an open vent. This model was maintained for 21 consecutive days to establish the anxiety model. The mice's heads were slightly elevated to facilitate urine excretion.
[0041] Several mice from which the anxiety model was successfully established were selected (positive group, group 1, group 2... group 8 were each prepared into a suspension with physiological saline), with 5 mice in each group, including: Model group: administered the same volume of physiological saline by gavage daily; Positive group: Daily gavage administration of diazepam 2.0 mg / kg; Group 1: Daily gavage administration of 0.5 g / kg of the coffee composition prepared in Example 1; Group 2: Daily gavage administration of 0.5 g / kg of the coffee composition prepared in Example 2; Group 3: 0.5 g / kg of the coffee composition prepared in Comparative Example 1 was administered by gavage daily; Group 4: 0.5 g / kg of the coffee composition prepared in Comparative Example 2 was administered via gavage daily; Group 5: 0.5 g / kg of the coffee composition prepared in Comparative Example 3 was administered via gavage daily; Group 6: Daily gavage administration of 0.5 g / kg of the coffee composition prepared in Comparative Example 4; Group 7: 0.5 g / kg of the coffee composition prepared in Comparative Example 5 was administered via gavage daily; Group 8: Daily gavage administration of 0.5 g / kg of the coffee composition prepared in Comparative Example 6; Five mice without an anxiety model were selected and administered the same dose of saline via gavage daily, serving as the control group. After 7 consecutive days of gavage, 2 hours after the last gavage, 3 mice were randomly selected from each group, and blood was collected by enucleation. The blood was centrifuged and the supernatant was used as the test solution.
[0042] The CORT (cortisol) content in the above test solution was determined according to the ELISA kit instructions, and the results are shown in Table 2.
[0043] Table 2. Blood CORT levels in different treatments
[0044] As shown in Table 2, after the model group developed anxiety, the CORT value was as high as 113.1 ng / mL. However, after gavage administration of the coffee composition in Examples 1 and 2, the CORT value in the mouse blood decreased significantly. This indicates that the coffee composition provided by the present invention is beneficial in relieving anxiety in mice.
[0045] In summary, this invention combines coffee beans, Eucommia ulmoides extract, oyster polypeptide powder, xylitol, and sorbitol. Eucommia ulmoides extract and oyster polypeptide, as food-grade and bioactive peptides, can inhibit excessive activation of the hypothalamus-pituitary-adrenal (HPA) axis, reducing abnormal secretion of corticotropin-releasing hormone and cortisol, thus normalizing elevated cortisol levels associated with chronic anxiety. Furthermore, the active ingredients are easily absorbed by the body, providing nutrition to nerve cells, repairing nerve cell membranes, and regulating nerve cell calcium homeostasis, effectively alleviating symptoms such as palpitations and tremors associated with anxiety. Therefore, this invention addresses both the root cause and symptoms, is non-drug-dependent, highly safe, and suitable for relieving anxiety.
[0046] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.
Claims
1. A coffee composition for use in alleviating anxiety, characterized in that, The coffee composition comprises the following ingredients in parts by weight: 15-20 parts coffee beans, 10-14 parts Eucommia ulmoides extract, 5-12 parts oyster polypeptide powder, 6-10 parts xylitol and 3-5 parts sorbitol.
2. The coffee composition according to claim 1, characterized in that, The preparation method of the oyster polypeptide powder includes: Oyster meat, flavor protease, and water were mixed and enzymatically hydrolyzed. After enzyme inactivation and solid-liquid separation, a filtrate was obtained. The filtrate was then concentrated to obtain oyster polypeptide powder.
3. The coffee composition according to claim 2, characterized in that, When performing mixed enzymatic hydrolysis, the mass ratio of oyster meat, flavor protease, and water is 10-15:5-8:85-90. The temperature for mixed enzymatic hydrolysis is 48-55℃, and the time is 8-10 hours.
4. The coffee composition according to claim 2, characterized in that, The preparation method of the Eucommia ulmoides extract includes: Eucommia ulmoides was extracted with supercritical CO2 with the aid of an entrainer to obtain Eucommia ulmoides residue; the residue was then extracted with an acidic solution and filtered to obtain an acid extract and the extracted residue; the extracted residue was mixed with water, cooked and filtered to obtain an aqueous extract and the cooked residue; the acid extract and aqueous extract were combined and then subjected to activated carbon adsorption, ethanol recovery and spray drying to obtain Eucommia ulmoides extract; The acidic solution includes ethanol and citric acid.
5. The coffee composition according to claim 4, characterized in that, The entrainer comprises a methanol solution with a volume concentration of 0.5%-2%.
6. The coffee composition according to claim 4, characterized in that, The supercritical CO2 extraction is performed at a pressure of 20-30 MPa, a temperature of 45-55℃, and a time of 15-20 min.
7. The coffee composition according to claim 4, characterized in that, When performing extraction, the volume-to-mass ratio of acidic solution to Eucommia ulmoides residue is 10-12 mL: 1 g; The extraction temperature is 35-40℃, and the time is 5-6 hours; The acidic solution uses water as a solvent and includes: ethanol with a volume concentration of 45%-55% and citric acid with a mass concentration of 0.5%-1%.
8. The coffee composition of claim 4, wherein, When steaming, the volume-to-mass ratio of water to the extracted Eucommia ulmoides residue is 10-12 mL: 1 g; The extraction temperature is 80-95℃ and the time is 40-60 minutes.
9. A method for preparing the coffee composition according to any one of claims 1-8, characterized in that, Includes the following steps: Coffee beans are roasted and ground to obtain coffee powder; Coffee powder, Eucommia ulmoides extract, oyster polypeptide powder, xylitol and sorbitol are mixed evenly to obtain a coffee composition.
10. The use of the coffee composition of claim 1 in the preparation of a health product or medicine for relieving anxiety.