A polyamino acid exchange chromatography medium based on end group fixation and its preparation method and application

By introducing end-group-fixed polyamino acid exchange chromatography media into macroporous polysaccharide microspheres, the problem of limited adsorption rate improvement in existing technologies has been solved, achieving highly efficient protein separation and purification, especially significantly improving adsorption capacity and mass transfer rate at low grafting density.

CN122321834APending Publication Date: 2026-07-03EAST CHINA UNIV OF SCI & TECH
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Filing Date
2026-05-11
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing polymer-grafted ion exchange chromatography media exhibit high chain flexibility at low grafting densities, resulting in limited improvement in adsorption rates and making it difficult to meet the requirements for efficient protein separation and purification.

Method used

The polyamino acid exchange chromatography medium based on end-group immobilization is used. By introducing polyanionic amino acids into the macroporous polysaccharide microsphere medium, the end groups react with epoxy groups to form unit sites for immobilization. The polyamino acid chains remain fully extended in the pores, providing abundant binding sites and high degrees of freedom. The binding chain transfer effect improves the mass transfer rate.

Benefits of technology

It significantly improved the static adsorption capacity and dynamic adsorption rate of proteins, achieving efficient protein separation and purification, with the diffusion coefficient reaching a maximum of 3.1×10-11 m2/s.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122321834A_ABST
    Figure CN122321834A_ABST
Patent Text Reader

Abstract

This invention relates to a polyamino acid exchange chromatography medium based on end-group immobilization, its preparation method, and its application. The preparation method includes the following steps: dispersing macroporous polysaccharide microspheres in a solvent to obtain a medium suspension; adding epichlorohydrin to the medium suspension and mixing thoroughly; adding sodium hydroxide solution to the medium suspension and stirring to obtain an epoxidized macroporous polysaccharide medium; immersing the epoxidized macroporous polysaccharide medium in an aqueous solution of polyanionic amino acids and stirring to promote the diffusion of polyanionic amino acids within the microspheres; adding sodium hydroxide solution and sodium borohydride solution to the mixture and stirring to obtain the polyamino acid exchange chromatography medium based on end-group immobilization. Compared with existing technologies, this invention utilizes end-group immobilized polyamino acid modification to prepare a macroporous cation exchange chromatography medium, significantly improving the protein adsorption capacity and rate of the chromatography medium.
Need to check novelty before this filing date? Find Prior Art