A high-yield erythritol-producing strain of Yarrowia lipolytica and its application

By constructing a ura3-deficient lipophilic yeast CA20△ura3 and knocking out the A000039 gene, the damage to cellular metabolic activity caused by high osmotic pressure stress was resolved, resulting in a significant increase in erythritol production. This simplified the gene modification process and reduced production costs.

CN122326416APending Publication Date: 2026-07-03ZHEJIANG UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV OF SCI & TECH
Filing Date
2026-05-21
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

In existing technologies, high osmotic pressure stress damages the metabolic activity of Yeast lipolyticis cells, resulting in low cost-effectiveness of erythritol production. Furthermore, multi-gene engineering modification is difficult to implement in industrial strains, limiting the increase in erythritol yield.

Method used

The ura3 gene-deficient lipophilic yeast CA20△ura3 was constructed by homologous recombination, and the A000039 gene was knocked out to obtain the engineered strain MS1. This simplified the gene modification process, and only one gene needs to be modified to significantly increase the yield of erythritol.

Benefits of technology

The yield of erythritol by shake-flask fermentation was increased from 125.25 g/L to 180.45 g/L, and the final concentration in the fermenter reached 222.45 g/L. The yield and productivity were significantly improved, resulting in good economic benefits.

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Abstract

This invention discloses a high-yielding erythritol-producing *Yarrowia lipophila* strain (… Yarrowia lipolytica This invention relates to the field of biotechnology, specifically to *Yarrowia lipophila* CA20 as the starting strain, and its applications. A000039 A gene knockout method was used to construct a high-yield erythritol-producing *Yarrowia lipolytica* strain. The construction method provided by this invention is simple and of significant value in simplifying the construction process of high-yield strains. Furthermore, under the fermentation medium and conditions provided by this invention, the *Yarrowia lipolytica* strain producing high-yield erythritol was fed-batch fermented with glucose as a substrate, achieving a yield of 280.50 g / L and a yield of 0.71 g / g, demonstrating good economic benefits.
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Citation Information

Patent Citations

  • Yarrowia lipolytica strain capable of producing erythritol at high yield as well as breeding method and application of Yarrowia lipolytica strain

    CN115305209A