A substance, a method of preparation and use in special food products

Abstract

This invention discloses a highly active and safe refined rubber seed oil, its preparation method, and its applications. The substance is obtained from crude rubber seed oil through alkali neutralization, natural sedimentation, centrifugation, adsorption filtration, and distillation. This substance not only exhibits high activity—superior to crude rubber seed oil in inhibiting atherosclerotic plaque formation and promoting plaque regression—but also demonstrates high safety, meeting national food safety standards. This substance can be applied to health foods, foods for special medical purposes, and other special foods for the health improvement and promotion of individuals with atherosclerosis.

Description

Technical Field

[0001] This invention belongs to the field of biology. Background Technology

[0002] Atherosclerotic plaques are the pathological basis of cardiovascular and cerebrovascular diseases and also an indicator of the health level of patients with these diseases. An increase in atherosclerotic plaques in patients with cardiovascular and cerebrovascular diseases indicates a deterioration in their health; conversely, a decrease in plaques indicates an improvement in their health.

[0003] Numerous studies have confirmed that both drug therapy and dietary management are crucial in managing cardiovascular and cerebrovascular diseases. Developing health foods and special medical purpose foods that benefit patients with cardiovascular and cerebrovascular diseases, and improving their health, is of great significance in helping them overcome these conditions.

[0004] Medical Research (Document 1. Liu Chaoran, Chen Guozhen, Li Yunshan, et al. Study on the regression of atherosclerosis in monkeys [J]. Science in China (Series B), 1987(02): 66-74; Document 2. Liu Chaoran, Yang Liang, Chen Guozhen, et al. Study on the effect of rubber seed oil on atherosclerosis - I. Effect on the formation and regression of experimental aortic atherosclerosis in rabbits [J]. Journal of Kunming Medical College, 1980(3); Document 3. Liu Chaoran, Li Yunshan, Chen Guozhen, et al. Effect of rubber seed oil on changes in blood lipids and cardiac function during the formation of atherosclerosis in monkeys [J]. Journal of Tropical Crops, 1985(2): 1. Document 4. Liu Chaoran, Chen Guozhen, Li Yunshan, Chen Longshun, Tang Chaocai, Zhou Shuyun, Zhang Zhixiong. Study on the preventive effect of rubber seed oil on atherosclerosis in monkeys [J]. Journal of Kunming Medical College, 1985(02):8-22. Document 5. Liu Hanjun, Zhang Hongfei, Liu Aiwu, et al. Ultrastructural observation of the effect of rubber seed oil on the prevention of experimental atherosclerosis in monkeys [J]. Journal of Kunming Medical University, 1986(2).) indicates that rubber seed oil has a significant inhibitory effect on the formation of atherosclerotic plaques and promotes the regression of atherosclerotic plaques, and can improve and promote the health of patients with cardiovascular and cerebrovascular diseases.

[0005] Documents 1-5 speculate that rubber seed oil is rich in unsaturated fatty acids, which have a lipid-lowering effect, and further speculate that this lipid-lowering effect leads to its reversal and regression effects on atherosclerosis. However, speculation is still speculation; it cannot confirm or deny the connection between the unsaturated fatty acids in rubber seed oil and its reversal and regression effects on atherosclerosis. To date, no research has revealed a link between specific components of rubber seed oil and its biological activity (the activity of reversing and regressing atherosclerosis).

[0006] Documents 1-5, being studies in the medical field, do not mention methods for preparing rubber seed oil. Based on the publication dates of these documents, the national conditions at the time, and our research, the rubber seed oil mentioned in these documents should be crude rubber seed oil or crude oil in food science, that is, a crude extract of the fat-soluble components of rubber seeds in phytochemistry.

[0007] Animal experiments have confirmed that crude rubber seed oil has significant biological activity in reversing and dissolving atherosclerosis, but it also contains many components that are detrimental to health and even toxic. Document 6 (Document 6: Luo Xiaolan, Zhu Wenxin, He Jian, et al. Research and practice of rubber seed oil refining [J]. Grain and Oil Processing, 2008, 000(011):46-49.) and Document 12 (Document 12: Li Linkai, Li Chen, Tao Yin. Research on short-path / molecular distillation process of rubber seed oil [J]. Grain and Oil Processing (Electronic Edition), 2015(09):26-28.) both point out that crude rubber seed oil has a high acid value and many complex impurities. In addition to mechanical impurities, phospholipids, mucilage, glycolipids and other impurities that are generally present in crude oil, it also contains some special impurities such as rubber, resin, lipoproteins, cyanogenic glycosides, etc., and even metal ion chelates due to its strong corrosiveness.

[0008] In food science, the process of removing harmful components from crude vegetable oils is called oil refining. Several food science papers have investigated various refining methods for crude rubber seed oils. The following are some of these research papers: Document 6: Luo Xiaolan, Zhu Wenxin, He Jian, et al. Research and practice on refining rubber seed oil [J]. Grain and Oil Processing, 2008, 000(011):46-49. Document 7: Shen Shandeng. Experimental study on physical refining of rubber seed oil [J]. China Oils and Fats, 1992(06):18-20. Document 8: Hu Xiaohong, Liu Dachuan, Zhang Xincai. Research on the extraction and refining process of rubber seed oil [J]. China Oils and Fats, 2005(11):66-68. Document 9: Wu Weizhong. Industrial uses of rubber seed oil [J]. China Oils and Fats, 1988(05):62-63. Document 10: Jia Wei. Rubber seed oil extraction technology and practice [J]. China Oils and Fats, 2006(02):12-14. Document 11: Zu Tingyue. Study on aqueous enzymatic extraction of rubber seed oil and its microemulsion preparation. Jiangnan University, 2013. Document 12: Li Linkai, Li Chen, Tao Yin. Research on short-path / molecular distillation process for rubber seed oil [J]. Grain and Oil Processing (Electronic Edition), 2015(09):26-28. Document 13: Guo Xiong. Research on the preparation of rubber seed oil and protein [D]. Wuhan University of Light Industry, 2018. Document 14: Lan Qinmu. Storage test report of refined rubber seed oil [J]. Oil and Fat Science, 1982(05):12-17. Document 15: Tian Hua, Huang Tao, Su Minghua. Research on degumming and decolorization process of rubber seed oil [J]. Journal of Wuhan University of Light Industry, 2007, 26(002):9-11. Document 16: Wang Xiaoli, Zhan Lin. Research on refining of rubber seed oil [J]. China Oils and Fats, 2000, 25(004):10-11. These refining methods differ, and the refined oils obtained can be either good or bad from a food science perspective. Food scientists typically consider the following aspects when judging the quality of a refining method: 1. Food safety: Does it meet the requirements of the "GB 2716-2018 National Food Safety Standard for Vegetable Oils"? 2. Finished product yield: With the raw material weight as 100%, what percentage of the final weight of the finished product can be obtained? Of course, the higher the yield, the better. 3. Production costs mainly include the complexity of the process, the level of equipment requirements, the length of the production cycle, the amount of energy and auxiliary materials consumed, etc. Of course, the more economical, the better.

[0009] Regardless of the food science merits or demerits of these refining methods, one thing is common: the rubber seed oils prepared by these methods are not linked to their biological activity, namely, their ability to inhibit atherosclerotic plaque formation and promote plaque regression. We do not know whether the rubber seed oils prepared by these different refining methods possess the effects of inhibiting atherosclerotic plaque formation and promoting plaque regression described in documents 1-5.

[0010] To investigate the effects of these refining methods on the biological activity of rubber seed oil (inhibition of atherosclerotic plaque formation and promotion of atherosclerotic plaque regression), this invention replicated the schemes in documents 6-15. Regardless of whether their safety standards were met, their yields were high or low, or their production costs were low, this invention found through extensive animal experiments that they had almost no biological activity. For details, please refer to Examples 4-9.

[0011] In 2016, the National Health Commission officially approved rubber seed oil as a food product, allowing it to be marketed, with hygiene and safety indicators implemented in accordance with relevant national standards. Currently, the hygiene and safety standard for vegetable oils in my country is the national standard GB 2716-2018, "National Food Safety Standard for Vegetable Oils." In other words, meeting the national standard GB 2716-2018 is a prerequisite for rubber seed oil to be marketed as a food product.

[0012] In summary, crude rubber seed oil has the effect of inhibiting the formation of atherosclerotic plaques and promoting their regression, possessing the potential to be developed into health foods and special medical purpose formula foods to improve and promote the health of patients with cardiovascular and cerebrovascular diseases. However, at the same time, crude rubber seed oil also contains a large number of substances that are detrimental to health and even toxic, failing to meet the standards of "GB 2716-2018 National Food Safety Standard for Vegetable Oils". Refined oil, after removing these harmful and even toxic components through oil refining, loses its biological activity, which is a major problem restricting the development and utilization of rubber seed oil. Summary of the Invention

[0013] This invention aims to solve the problem of developing and utilizing rubber seed oil. While meeting the safety requirements in food science, it also takes into account the biological activity of rubber seed oil in inhibiting the formation of atherosclerotic plaques and promoting the regression of atherosclerotic plaques. This will truly transform rubber seed oil into a health food, a special food for special medical purposes, and other special foods that improve and promote the health of people with atherosclerosis.

[0014] One of the objectives of this invention is to provide a novel rubber seed oil that, unlike any other in the prior art, meets the safety standards of the "GB 2716-2018 National Food Safety Standard for Vegetable Oils" while possessing biological activity (inhibiting the formation of atherosclerotic plaques and promoting the regression of atherosclerotic plaques) that reaches or even surpasses that of crude raw oil.

[0015] The second objective of this invention is to provide a method for preparing this highly active and safe refined rubber seed oil.

[0016] The third objective of this invention is to provide the application of this highly active and safe refined rubber seed oil in the production of special foods such as health foods and foods for special medical purposes. This substance can be used in health foods and foods for special medical purposes to improve and promote the health of individuals with atherosclerosis.

[0017] To achieve the above objectives, the present invention provides the following technical solution: A highly active and safe refined rubber seed oil is characterized in that the substance is prepared by the following method: using crude rubber seed oil as raw material, it is obtained by neutralization with alkali, natural sedimentation, centrifugal separation, adsorption filtration, and deodorization.

[0018] This invention also provides a method for preparing this highly active and safe refined rubber seed oil: using crude rubber seed oil as raw material, it is obtained by neutralizing with alkali, natural sedimentation, centrifugal separation, adsorption filtration, and deodorization.

[0019] In the neutralization step, food-grade alkali is used. The amount of alkali added is equal to the molar mass of the fatty acids contained in the crude oil. The alkali is added in the form of an aqueous solution and then mixed.

[0020] The natural sedimentation process is as follows: the mixture is allowed to stand at a temperature above 70°C until the fatty acid salts and oil phases are clearly separated. The lower fatty acid salt phase is then discarded, and the oil phase is retained.

[0021] The centrifugal separation step is performed under the following conditions: oil phase temperature above 70℃, centrifugal force greater than or equal to 5000g, and time not less than 1 minute.

[0022] To achieve the conditions for such centrifugal separation in large-scale industrial production, the present invention also provides a specific method for implementation: centrifugation is performed using a disc centrifuge or a tubular centrifuge, the centrifugation temperature of the oil phase is 70℃-100℃, the centrifugal force is 5000-30000g, preferably 6000-18000g, the remaining fatty acid salts are discarded, and the oil phase is separated.

[0023] Oily substances are adsorbed, filtered, and deodorized; what remains is the final product.

[0024] This invention provides a health food product containing an effective dose of highly active and safe refined rubber seed oil and a health food-acceptable carrier.

[0025] This invention provides the application of highly active and safe refined rubber seed oil in the preparation of health food products for people with atherosclerosis. In this application, the highly active and safe refined rubber seed oil is the sole active ingredient in the health food product.

[0026] This invention provides a food for special medical purposes consisting of an effective dose of highly active and safe refined rubber seed oil and a food for special medical purposes acceptable carrier.

[0027] This invention provides the application of highly active and safe refined rubber seed oil in the preparation of special medical purpose foods for people with atherosclerosis. In this application, the highly active and safe refined rubber seed oil is the sole active ingredient in the special medical purpose food.

[0028] This invention provides other special foods for specific populations, consisting of an effective dose of highly active and safe refined rubber seed oil and an acceptable carrier.

[0029] This invention provides the application of highly active and safe refined rubber seed oil in the preparation of other special foods for people with atherosclerosis. In this application, the highly active and safe refined rubber seed oil is the sole active ingredient in the other special foods.

[0030] Compared with existing methods, the rubber seed oil obtained by this method has a fundamental difference in activity and a fundamental difference in the method of implementation.

[0031] Based on experimental data, the present invention has the following advantages: This invention provides a novel substance that, while meeting the safety standards of GB 2716-2018 National Food Safety Standard for Vegetable Oils (Examples 1-2), exhibits activity that can inhibit the formation of atherosclerotic plaques and promote the regression of atherosclerotic plaques, reaching or even exceeding that of crude rubber seed oil (Examples 10-11) used as a raw material.

[0032] This invention also provides a method for the industrial-scale production of this highly active and safe refined rubber seed oil, rather than being limited to laboratory preparation (Examples 1-2).

[0033] The substantive content of the present invention will be further described below with reference to the accompanying drawings and embodiments, but this is not intended to limit the present invention. Attached Figure Description

[0034] Figure 1 The atherosclerotic lesion levels of mice in different groups are shown in Example 9.

[0035] Figure 2 The image shows a typical atherosclerotic plaque tracing of the rabbit aorta in Example 10. From left to right, the images were taken from the normal diet group, the high-fat diet group, the rubber seed crude oil + high-fat diet group, the present invention + high-fat diet group, and the simvastatin + high-fat diet group. Figure 3 This shows the proportion of atherosclerotic plaques in the aortic area of ​​rabbits in different groups in Example 10; Figure 4 The p-values ​​for the differences between different groups in Example 10 are shown. A p-value less than 0.05 is considered a significant difference, and a p-value less than 0.01 is considered a highly significant difference. Figure 5 This shows the proportion of atherosclerotic plaques in the aorta of rabbits in different groups in Example 10.

[0036] Figure 6The image shows a typical atherosclerotic plaque tracing of the rabbit aorta in Example 11, taken from left to right in the following order: normal diet group, atherosclerosis model group, model + normal feeding group, model + crude oil group, model + the present invention group, and model + simvastatin group. Figure 7 This shows the proportion of atherosclerotic plaques in the aortic area of ​​rabbits in different groups in Example 11; Figure 8 The p-values ​​for the differences between different groups in Example 11 are shown. A p-value less than 0.05 is considered a significant difference, and a p-value less than 0.01 is considered a highly significant difference. Figure 9 This shows the proportion of atherosclerotic plaques in the aorta of rabbits in different groups in Example 11.

[0037] Example 1 Highly active and highly safe rubber seed oil is prepared according to the method of the present invention.

[0038] Using 1000 kg of crude rubber seed oil as raw material, with a measured acid value of 30 mg KOH / g, 21.4 kg of solid NaOH was required. The solid alkali was dissolved in 78.6 kg of water to prepare 100 kg of alkali solution with a weight percentage concentration of 21.4%. The crude oil was heated to 90℃, then slowly stirred. The alkali solution was slowly added to the crude oil, and stirring continued until the crude oil and alkali solution reacted completely. Stirring was then stopped, and the oil was allowed to settle naturally while maintaining the oil temperature. After 4 hours of natural settling, the oil phase and fatty acid salt phase were found to be clearly separated. The fatty acid salt phase was discarded, and the oil phase temperature was adjusted to 90℃. The oil was then centrifuged in a disc centrifuge at a centrifugal force of 6000 g and a residence time of 1.5 minutes. The centrifuged oil phase was then vacuum-heated for dehydration at a vacuum pressure of 10 kPa and a heating temperature of 100℃ for 60 minutes. Then, 1.2% (by weight) of an adsorbent (activated clay) was added, and the mixture was stirred for 10 minutes. The mixture was then filtered to obtain clear oil. The oil was heated to 180℃ and deodorized for 30 minutes under a vacuum of 1 kPa by introducing steam. The process was then stopped, the oil was cooled, and the pressure was released to obtain 500 kg of the finished product, with a yield of 50%. The product is pale yellow in color, a clear, transparent oily liquid, free of foreign matter, and has the inherent odor and taste of rubber seed oil, without any off-odors.

[0039] The finished products were tested according to the standards of GB 2716-2018 National Food Safety Standard for Vegetable Oils, and all passed the tests. Below are some of the test results:

[0040] This indicates that the safety of the rubber seed oil of this invention has reached the national safety standards for food (edible vegetable oil).

[0041] Example 2 Highly active and highly safe rubber seed oil is prepared according to the method of the present invention.

[0042] Using 1000 kg of crude rubber seed oil as raw material, with a measured acid value of 30 mg KOH / g, 21.4 kg of solid NaOH was required. The solid alkali was dissolved in 78.6 kg of water to prepare 100 kg of alkali solution with a weight percentage concentration of 21.4%. The crude oil was heated to 80°C, then slowly stirred. The alkali solution was slowly added to the crude oil, and stirring continued until the crude oil and alkali solution reacted completely. Stirring was then stopped, and the oil was allowed to settle naturally while maintaining the oil temperature. After 6 hours of natural settling, a clear separation of the oil phase and fatty acid salt phase was observed. The fatty acid salt phase was discarded, and the oil phase was adjusted to 70°C and centrifuged in a tubular centrifuge at a centrifugal force of 18000 g for a residence time of 1.5 minutes. The oil phase after centrifugation was dehydrated under vacuum heating at 10 kPa and 100°C for 60 minutes. Then, 1.5% (by weight) of an adsorbent (a mixture of activated clay and diatomaceous earth) was added, and the mixture was stirred for 10 minutes. The mixture was then filtered to obtain the clear oil. The clear oil was heated to 105°C and deodorized for 30 minutes under a 0.2 kPa vacuum by introducing steam. The process was then stopped, the temperature was lowered, and the pressure was released, yielding 520 kg of the final product, with a yield of 52%. The product is pale yellow, clear, transparent, and oily, free of impurities, and has the inherent odor and taste of rubber seed oil, without any off-odors.

[0043] The finished products were tested according to the standards of GB 2716-2018 National Food Safety Standard for Vegetable Oils, and all passed the tests. Below are some of the test results:

[0044] This indicates that the safety of the rubber seed oil of this invention has reached the national safety standards for food (edible vegetable oil).

[0045] Example 3 Explore the conditions and methods for achieving centrifugal separation in large-scale industrial production.

[0046] 3.1 Direct centrifugation using a disc centrifuge. Using 1000 kg of crude rubber seed oil as raw material, with a measured acid value of 30 mg KOH / g, 21.4 kg of solid NaOH was calculated to be required. The solid alkali was dissolved in 78.6 kg of water to prepare 100 kg of an alkali solution with a weight percentage concentration of 21.4%. The crude oil and alkali solution were mixed in a 10:1 ratio, heated to 80°C, and then injected into a disc centrifuge for centrifugation. The experiment failed regardless of adjustments to the flow rate, centrifugal force, temperature, or other technical parameters.

[0047] 3.2 Direct centrifugation using a tubular centrifuge. Using 1000 kg of crude rubber seed oil as raw material, with a measured acid value of 30 mg KOH / g, 21.4 kg of solid NaOH was required. The solid alkali was dissolved in 78.6 kg of water to prepare 100 kg of an alkali solution with a weight percentage concentration of 21.4%. The crude oil and alkali solution were mixed in a 10:1 ratio, heated to 80°C, and then injected into a tubular centrifuge. Continuous separation was performed at 10000 g, with the flow rate adjusted to ensure a residence time of 1.5 minutes. The separated oil phase was then subjected to vacuum heating for dehydration, followed by adsorption filtration. Filtration failed, resulting in separation failure. No matter how the flow rate, the ratio of crude oil to alkali solution, the centrifugal force, or the temperature were adjusted, all technical parameters failed.

[0048] 3.3 Centrifugal Force. Using 1000 kg of crude rubber seed oil as raw material, with a measured acid value of 30 mg KOH / g, 21.4 kg of solid NaOH was required. The solid alkali was dissolved in 78.6 kg of water to prepare 100 kg of alkali solution with a weight percentage concentration of 21.4%. The crude oil was heated to 80°C, then slowly stirred. The alkali solution was slowly added to the crude oil, and stirring continued until the crude oil and alkali solution reacted completely. Stirring was then stopped, and the oil was allowed to settle naturally while maintaining the oil temperature. After 6 hours of natural settling, the oil phase and fatty acid salt phase were found to be clearly separated. The fatty acid salt phase was discarded, and the oil phase was heated to 85°C and then injected into a tubular or disc centrifuge for centrifugal separation. The centrifugal force was gradually increased in 1000 g increments from 1000 g to 40000 g. It was found that effective separation could not be achieved below 5000 g; effective separation was possible above 5000 g. Increasing the centrifugal force increased the processing efficiency of the equipment. When the centrifugal force exceeds 30,000g, the requirements for equipment materials, processing precision, wear rate, and operational difficulty all increase significantly, while the benefits gained are limited. The optimal overall performance is achieved when the centrifugal force is between 6,000 and 18,000g.

[0049] Example 4 Refined rubber seed oil was prepared according to the method in document 6. Using 1000 kg of crude rubber seed oil as raw material, the measured acid value was 30 mg KOH / g. Following the optimized method in document 6, the oil temperature was adjusted to 40℃, 3 kg of 85% phosphoric acid was added, and after thorough mixing, the temperature was raised to 80℃ with rapid stirring. Then, 80 kg of softened water (water with calcium and magnesium ions removed) at 80℃ was added. After adding water, the stirring speed was reduced to slow, and the temperature was raised to 85℃. Stirring continued for 30 minutes, then stopped. The mixture was allowed to settle for at least 6 hours. The oil residue, wastewater, and sludge were discharged, and then vacuum dehydration was performed. The vacuum dehydration pressure was 0.005 MPa (0.08 MPa-0.075 MPa), and the temperature was 90℃. After dehydration, the oil temperature was cooled to below 40℃ under vacuum. The pre-decolorized oil was heated to 110℃, and 25 kg of activated clay was added under a vacuum of 0.005 MPa (0.08 MPa-0.075 MPa), along with 1.25 kg of activated carbon. Halfway through the secondary decolorization filtration, the process was switched to pre-decolorization of the raw oil. When the pressure of the leaf filter reached its upper limit, filtration was stopped, and the filter cake was blown off to complete one filtration cycle. The entire filtration process was difficult and slow. The decolorized oil entered a packed deacidification tower at a pressure of ≤100 Pa. The temperature of the oil entering the tower was heated to 240-250℃, and the outlet temperature was controlled at ≥230℃. The outlet acid value was controlled to ≤1 mg KOH / g. The oil was cooled to room temperature, yielding 810 kg of refined oil product prepared by the method in document 6, with a yield of 81%. This refined oil was red, clear, transparent, and odorless.

[0050] The refined oil was tested according to the standard of "GB 2716-2018 National Food Safety Standard for Vegetable Oils" and was determined to be a compliant product.

[0051] Example 5 Refined rubber seed oil was prepared according to the method in document 10. Using 1000 kg of crude rubber seed oil as raw material, the measured acid value was 30 mg KOH / g. Following the method in document 10, the oil was heated to 60℃, 2 kg of 85% phosphoric acid was added, and the reaction was allowed to proceed for 18 minutes. After settling, the lower layer of liquid was released, and the oil was washed once with water, resulting in significant emulsification. The oil was then heated to 65℃, 1 kg of formic acid was added, and the reaction was allowed to proceed for 20-25 minutes. After settling, the oil was washed three times with water, each time showing significant emulsification, and the oil turned milky white. The oil was then heated to 112℃ under a vacuum of 0.6 kPa to dry and dehydrate. After dehydration, 40 kg of activated clay and 10 kg of activated carbon were added, and the mixture was stirred at 100℃ for 30 minutes. The oil was then filtered while hot to obtain the clear oil. Filtration was difficult and the filtration speed was very slow. The filtered clear oil was extracted with 75% ethanol at an oil:ethanol ratio of 1:1.5 for the first extraction. The mixture was stirred at 58°C, then allowed to stand and separate into layers, separating the lower oil phase. A second extraction was then performed under the same conditions. This process was repeated 15 times until the acid value dropped below 3, at which point the extraction was terminated. The oil phase was then heated under negative pressure to evaporate the ethanol, yielding 690 kg of the refined oil product prepared by the method described in document 10, with a yield of 69%. This refined oil was red, clear, and transparent; it emitted an ethanol odor after prolonged storage in a container.

[0052] The refined oil was tested according to the standard of "GB 2716-2018 National Food Safety Standard for Vegetable Oils". Two indicators failed the test, while the remaining indicators were qualified. The unqualified indicators were: odor and taste, and solvent residue.

[0053] Example 6 Refined rubber seed oil was prepared according to the method in document 12. Using 1000g of crude rubber seed oil as raw material, the measured acid value was 30mgKOH / g. Following the optimized method in document 12, the oil temperature was adjusted to 40℃, 3g of 85% phosphoric acid was added, and after thorough mixing, the temperature was raised to 80℃ with rapid stirring. 80g of softened water (water with calcium and magnesium ions removed) at 80℃ was added, and the stirring speed was reduced to slow. The temperature was raised to 85℃, and stirring continued for 30 minutes. Stirring was then stopped, and the mixture was allowed to settle for at least 6 hours. The upper oil layer was separated from the lower oil residue and wastewater, and then dehydrated under normal pressure. Under normal pressure, the oil temperature was heated to 110℃ until no more bubbles appeared. After dehydration, the oil was cooled to below 40℃. The oil was then heated to 110℃, 50g of activated clay was added, along with 2.5g of activated carbon. The mixture was stirred for 30 minutes and then filtered through three layers of filter paper. To accelerate the filtration process, a Buchner funnel was used for suction filtration. Despite this, the filtration rate remains slow. The filtered oil then enters the molecular distillation apparatus for molecular distillation. The conditions for molecular distillation are: feed pump rotation frequency 20Hz, scraper rotor speed 300rpm, coolant temperature 25℃, heat transfer oil temperature 200℃, and vacuum pump vacuum degree 1.33Pa.

[0054] Following the method in document 12 directly for one round of distillation, the acid value was still as high as 12 mg KOH / g, exceeding the standard of 3 mg KOH / g in "GB 2716-2018 National Food Safety Standard for Vegetable Oils". After repeating three rounds of molecular distillation, the acid value dropped below 3 mg KOH / g, and finally, 830 grams of the product prepared by the method in document 12 were obtained, with a yield of 83%.

[0055] The refined oil was tested according to the standard of "GB 2716-2018 National Food Safety Standard for Vegetable Oils" and was determined to be a compliant product.

[0056] Example 7 Refined rubber seed oil was prepared according to the method in document 14. Using 1000 kg of crude rubber seed oil as raw material, the measured acid value was 30 mg KOH / g. The crude oil was heated to 70°C, and 3 kg of 85% phosphoric acid and 20 kg of saturated NaCl aqueous solution were added. After standing, the lower layer of hydrates was separated, and the upper oil phase was retained. 21.4 kg of solid NaOH was added to 78.6 kg of saturated NaCl solution to prepare 100 kg of a 21.4% NaOH-saturated NaCl solution (hereinafter referred to as alkali-sodium chloride solution). 100 kg of the alkali-sodium chloride solution was added to the oil phase, and the entire reaction system was heated to 70°C. At this point, the oil phase and the alkali-sodium chloride solution separated into two layers, and no acid-base neutralization reaction occurred. To allow the oil phase to contact and react with the alkaline solution, a slow stirring method was used. The alkaline-sodium chloride solution gradually came into contact with the oil phase, resulting in an acid-base neutralization reaction and producing a large amount of sodium fatty acids. This led to emulsification, where the oil phase, sodium fatty acids, alkaline solution, and sodium chloride solution emulsified together to form a brown, viscous, and homogeneous emulsion system. After standing at this temperature for 24 hours, the upper emulsion was taken and washed with saturated brine at 100°C, but severe emulsification persisted. After standing at this temperature for another 24 hours, no obvious stratification occurred. The upper emulsion was then heated to 120°C for drying and dehydration, which was extremely difficult, with a large amount of foam overflowing. After dehydration, 120 kg of refined oil according to the method in document 14 was obtained, with a yield of 12%. This refined oil was a viscous, jelly-like, reddish-black, turbid substance with a strong off-odor.

[0057] The refined oil was tested according to the standard of "GB 2716-2018 National Food Safety Standard for Vegetable Oils" and was determined to be substandard.

[0058] Example 8 Refined rubber seed oil was prepared according to the method in document 13. Using 1000g of crude rubber seed oil as raw material, the measured acid value was 30mgKOH / g. Following the optimized method in document 13, 1000g of crude rubber seed oil was dissolved in 1703g of n-hexane to prepare a 37% (w / w) crude rubber seed oil-n-hexane solution. A 10kDa filter membrane was used, and the crude oil-n-hexane solution was passed through the membrane under a positive pressure of 0.23MPa. The filtered crude oil-n-hexane solution was heated, and the n-hexane solvent was removed under negative pressure, yielding 950g of the product prepared by the method in document 13, with a product yield of 95%. This product is a dark red, opaque oily liquid, not significantly different from the crude oil raw material.

[0059] The product was tested according to the standard of "GB 2716-2018 National Food Safety Standard for Vegetable Oils". The results were similar to those of direct testing of crude oil, with many indicators failing to meet the standards. In particular, the acid value was as high as 27 mg KOH / g. No matter how many times it was filtered through the membrane, the acid value remained above 25 mg KOH / g, with no possibility of reducing it to below 3 mg KOH / g. The substance obtained by this method showed little difference in physicochemical properties from crude oil.

[0060] Example 9 Animal experiments were conducted on the effects of crude rubber seed oil, the rubber seed oil of the present invention, and the rubber seed oil prepared in Examples 4 (Document 6), 5 (Document 10), 6 (Document 12), and 7 (Document 14) on the formation of atherosclerotic plaques. The physicochemical properties of the rubber seed oil prepared in Example 8 (Document 13) were not significantly different from those of the crude rubber seed oil, therefore it was not included in the animal experiments.

[0061] Twelve 8-week-old male wild-type C57BL / 6J mice were selected; 84 8-week-old male APOE mice were selected. - / - Mice, weighing 24±2g. APOE - / - Mice were randomly divided into 7 groups of 12 mice each, plus 12 wild-type C57BL / 6J mice, for a total of 8 groups. The grouping is as follows: (1) Normal diet group: 12 eight-week-old male wild-type C57BL / 6J mice were fed with basal diet and gavage with physiological saline. (2) High-fat diet group: 12 APOEs - / - Mice were fed a high-fat diet and administered peanut oil by gavage at a dose of 6.2 g / kg / day (calculated based on adult and animal weight, body surface area, and Km factor). (3) Rubber seed crude oil + high-fat diet group: 12 APOEs - / - Mice were fed a high-fat diet and administered rubber seed crude oil by gavage at a dose of 6.2 g / kg / day (calculated based on adult and animal weight, body surface area, and Km factor). (4) Invention Group + High-Fat Diet Group: 12 APOEs - / - Mice were fed a high-fat diet and administered the preparations from Examples 1-2 by gavage at a dose of 6.2 g / kg / day (calculated based on adult and animal weight, body surface area, and Km factor). (5) Document 6 group + high-fat diet group: 12 APOEs - / - Mice were fed a high-fat diet and administered the preparation of Example 4 by gavage at a dose of 6.2 g / kg / day (calculated based on adult and animal weight, body surface area, and Km factor). (6) Document 10 group + high-fat diet group: 12 APOEs - / - Mice were fed a high-fat diet and administered the preparation of Example 5 by gavage at a dose of 6.2 g / kg / day (converted based on adult and animal weight, body surface area, and Km factor). (7) Document 12 group + high-fat diet group: 12 APOEs - / - Mice were fed a high-fat diet and administered the preparation of Example 6 by gavage at a dose of 6.2 g / kg / day (calculated based on adult and animal weight, body surface area, and Km factor). (8) Document 14 group + high-fat diet group: 12 APOEs - / - Mice were fed a high-fat diet and administered the preparation of Example 7 by gavage at a dose of 6.2 g / kg / day (converted based on adult and animal weight, body surface area, and Km factor). All mice in the above groups were administered the diet via gavage for 8 consecutive weeks. The high-fat diet formula consisted of: 70% basal feed, 20% lard, 5% sucrose, 4% milk powder, 1% bile salts, and 0.15% cholesterol.

[0062] Mice were euthanized, their thoracic cavities were opened, and their blood vessels were perfused with pre-cooled physiological saline. The aorta was dissected, cut off intact, rinsed with PBS, and fixed in 10% formaldehyde solution. The degree of atherosclerosis was assessed using a grading method. Grade 0: The inner membrane surface is smooth, with no creamy discoloration, i.e., no plaques; Grade 0.5: The inner membrane has a wide range of cream or milky white variations, but no patches protruding from the surface; Grade 1: The intima has obvious cream-colored raised patches, with a patch area of ​​less than 3 mm. 2 ; Grade 2: The inner membrane has obvious cream-colored raised patches, but there is no merging into larger patches. The largest patch is larger than 3 mm in area. 2 ; Grade 3: Numerous patches of varying sizes, some merging into larger areas; large patches exceeding 3 mm in area. 2 Grade 4: The arterial intima is almost entirely covered by fused plaques.

[0063] The results of the animal experiments are shown in the table below. Figure 1 :

[0064] Experiments show that: (1) The rubber seed oil of the present invention has the effect of inhibiting the formation of atherosclerotic plaques. After adding the rubber seed oil of the present invention, the degree of atherosclerosis in APOE- / - mice on a high-fat diet was grade 1; the degree of atherosclerosis in APOE- / - mice on a high-fat diet without the addition of the rubber seed oil was grade 3-4; after adding the rubber seed oil of the present invention, the degree of atherosclerosis development in APOE- / - mice was significantly slowed down, indicating that the rubber seed oil of the present invention has the effect of inhibiting the formation of atherosclerotic plaques; (2) The rubber seed oils prepared in Examples 4, 5, 6, and 7 did not show significant inhibitory effects on the formation of atherosclerotic plaques. After adding the rubber seed oils prepared in Documents 6 (Example 4), 10 (Example 5), 12 (Example 6), and 14 (Example 7), the degree of atherosclerosis in APOE- / - mice on a high-fat diet was grade 3-4; the degree of atherosclerosis in APOE- / - mice on a high-fat diet without the addition of the rubber seed oils was also grade 3-4. Regardless of whether the rubber seed oils prepared in Documents 6 (Example 4), 10 (Example 5), 12 (Example 6), and 14 (Example 7) were added, the degree of atherosclerosis development in APOE- / - mice did not change significantly, indicating that the rubber seed oils prepared in Documents 6 (Example 4), 10 (Example 5), 12 (Example 6), and 14 (Example 7) did not show any inhibitory effect on the formation of atherosclerotic plaques.

[0065] Example 10 Animal experiments on the effects of crude rubber seed oil, the rubber seed oil of the present invention, and simvastatin on the formation of atherosclerotic plaques.

[0066] Rabbits are an ideal animal model for dietary-induced atherosclerosis research, as their pathogenesis is closer to that of humans than that of genetically defective mice and rats. Forty 4-month-old male Japanese White rabbits weighing 2.0 ± 0.2 kg were randomly divided into 5 groups as follows: (1) Normal diet group: 8 male Japanese white rabbits were fed a basic diet; (2) High-fat diet group: 8 male Japanese white rabbits were fed peanut oil + high-fat fortified + basic feed. The peanut oil feeding dosage was 1.5g / kg / d (calculated based on adult and animal weight, body surface area and Km factor). (3) Rubber seed crude oil + high-fat diet group: 8 male Japanese white rabbits were fed rubber seed crude oil + high-fat fortification + basic feed. The rubber seed crude oil feeding dose was 1.5g / kg / d (calculated based on adult and animal weight, body surface area and Km factor). (4) This invention + high-fat diet group: 8 male Japanese white rabbits were fed with the preparations of Example 1-2 + high-fat fortification + basic feed. The feeding dose of the preparations of Example 1-2 was 1.5g / kg / d (calculated based on the weight, body surface area and Km factor of adults and animals). (5) Simvastatin + high-fat diet group: 8 male Japanese white rabbits were fed simvastatin + high-fat fortification + basic diet. The simvastatin feeding dose was 2.0 mg / kg / d (calculated based on adult and animal weight, body surface area and Km factor). The high-fat fortified diet consists of 0.5 grams of cholesterol, 2.0 grams of lard, and 20 grams of egg yolk per rabbit per day.

[0067] The animals were fed for 6 weeks according to the above grouping and feeding conditions, and then sacrificed. After sacrifice, the aorta was completely removed from the aortic valve orifice to the origin of the common iliac artery. The aortic wall was longitudinally cut along the midline of the abdomen, fixed with 10% formaldehyde solution, and the distribution map of aortic lesions was recorded according to the tracing method. The area of ​​atherosclerotic plaques and the total area of ​​the expanded lumen of the aorta were measured, and the percentage of plaque area was calculated.

[0068] The experimental results are as follows: see Figure 2 , Figure 3 , Figure 4 , Figure 5 Experimental results show that: (1) The rubber seed oil of the present invention has a significant effect on inhibiting the formation of atherosclerotic plaques (the plaque area of ​​the present invention + high-fat diet group was 14.7±4.2%, which was smaller than that of the high-fat diet group (42.6±9.7%), P<0.01, the difference was extremely significant). (2) The rubber seed oil of the present invention has a stronger effect on inhibiting the formation of atherosclerotic plaques than the crude rubber seed oil (the plaque area of ​​the present invention + high-fat diet group was 14.7±4.2%, which was smaller than that of the crude rubber seed oil + high-fat diet group (19.7±4.7%, P<0.05, the difference was significant). (3) The rubber seed oil of the present invention, at a dose of 1.5 g / kg / d, has a stronger effect on inhibiting the formation of atherosclerotic plaques than simvastatin at a dose of 2.0 mg / kg / d (the plaque area of ​​the present invention + high-fat diet group was 14.7±4.2%, which was smaller than that of the simvastatin + high-fat diet group (20.2±4.2%, P<0.05, the difference was significant).

[0069] Example 11 Animal experiments on the effects of crude rubber seed oil, the rubber seed oil of the present invention, and simvastatin on established atherosclerotic plaques.

[0070] Rabbits are an ideal animal model for dietary-induced atherosclerosis research, as their pathogenesis is closer to that of humans than that of genetically defective mice and rats. Forty 4-month-old male Japanese White rabbits weighing 2.0 ± 0.2 kg were randomly divided into two groups as follows: (1) Normal diet group: 8 male Japanese white rabbits were fed a basic diet; (2) Atherosclerosis model group: 40 male Japanese white rabbits were fed a high-fat fortified diet plus a basic diet, and peanut oil was fed at a dose of 1.5 g / kg / d (calculated based on adult and animal weight, body surface area and Km factor). The high-fat fortified diet consisted of 0.5 grams of cholesterol, 4.0 grams of lard, and 20 grams of egg yolk per rabbit per day. The rabbits were fed this diet for 6 weeks, during which one rabbit in the normal diet group died.

[0071] Six weeks later, eight rabbits from the atherosclerosis model group were randomly selected and sacrificed to verify the successful establishment of the atherosclerosis model. The verification method was as follows: After sacrifice, the aorta was completely removed from the aortic valve orifice to the origin of the common iliac artery. The aortic wall was longitudinally cut along the midline of the abdomen, fixed with 10% formaldehyde solution, and the distribution of aortic lesions was recorded using a tracing method. The area of ​​atherosclerotic plaques and the total area of ​​the expanded lumen of the aorta were measured, and the percentage of plaque area was calculated. As a control, eight rabbits from the normal diet group were also sacrificed, and the percentage of aortic atherosclerotic plaque area was measured.

[0072] After confirming the successful establishment of the atherosclerosis model in the rabbit group, the remaining 32 rabbits in the model group were randomly divided into 4 groups of 8 rabbits each, as follows: (3) Modeling + Normal Feeding Group: Eight male Japanese white rabbits that successfully developed atherosclerosis were fed peanut oil + basal feed. The peanut oil feeding dosage was 1.5g / kg / d (calculated based on adult and animal weight, body surface area and Km factor). (4) Modeling + Rubber Seed Oil Group: Eight male Japanese white rabbits that successfully developed atherosclerosis were fed rubber seed oil + basic feed. The dosage of rubber seed oil was 1.5 g / kg / d (calculated based on adult and animal weight, body surface area and Km factor). (5) Modeling + Invention Group: Eight male Japanese white rabbits that successfully modeled atherosclerosis were fed with the preparations of Examples 1-2 plus basic feed. The feeding dose of the preparations of Examples 1-2 was 1.5 g / kg / d (calculated based on adult and animal weight, body surface area and Km factor). (6) Modeling + Simvastatin group: Eight male Japanese white rabbits that successfully modeled atherosclerosis were fed simvastatin + basal diet. The simvastatin feeding dose was 2.0 mg / kg / d (converted based on adult and animal weight, body surface area and Km factor). Continue feeding according to the above grouping and feeding conditions for 3 months. During this period, one rabbit died in each of the modeling + normal feeding group and the modeling + simvastatin group. After continuing feeding for 3 months, all animals were euthanized.

[0073] After the animal was euthanized, the aorta was completely removed from the aortic valve orifice to the origin of the common iliac artery. The aortic wall was cut longitudinally along the midline of the abdomen, fixed with 10% formaldehyde solution, and the distribution map of aortic lesions was recorded according to the tracing method. The area of ​​atherosclerotic plaques and the total area of ​​the expanded lumen of the aorta were measured, and the percentage of plaque area was calculated.

[0074] The experimental results are as follows: see Figure 6 , Figure 7 , Figure 8 , Figure 9 Experimental results show that: (1) The rubber seed oil of the present invention can not only inhibit the formation of atherosclerotic plaques (the plaque area in the model + present invention group was 27.5±6.8%, which was smaller than that in the model + normal feeding group (59.9±8.9%, P<0.01, the difference was extremely significant), but also promote the regression of existing atherosclerotic plaques (the plaque area in the model + present invention group was 27.5±6.8%, which was smaller than that in the model group (45.8±9.9%, P<0.01, the difference was extremely significant); (2) The rubber seed oil of the present invention is more effective than crude rubber seed oil in inhibiting the formation of atherosclerotic plaques (the plaque area in the model + present invention group was 27.5±6.8%, which was less than that in the model + crude oil group (36.2±7.9%, P<0.05, the difference was significant). (3) The rubber seed oil of the present invention, at a dose of 1.5 g / kg / d, has a better effect on inhibiting the formation of atherosclerotic plaques than simvastatin at a dose of 2.0 mg / kg / d (the plaque area in the present invention group was 27.5±6.8%, which was less than that in the simvastatin group of 47.1±8.3%, P<0.01, and the difference was extremely significant).

[0075] Example 12: The highly active and safe substance of the present invention was prepared according to the method in Examples 1-2. Excipients were added to this substance at a weight ratio of 1:1 or 1:2 to the excipients, and the mixture was granulated and compressed into tablets. The tablets can be used as health foods, foods for special medical purposes, or other special foods.

[0076] Example 13: The highly active and safe substance of the present invention was prepared according to the method in Examples 1-2. This substance was then encapsulated in a soft capsule material and formulated into soft capsules using conventional capsule preparation methods. The soft capsules can be used as health foods, foods for special medical purposes, or other special foods.

[0077] Example 14: The highly active and highly safe refined rubber seed oil of the present invention was prepared according to the method in Examples 1-2. Then, powdered oil wall material, emulsifier, osmotic pressure regulator, and water were added, followed by high-pressure homogenization emulsification and spray drying to obtain a powder (powdered oil). The powder (powdered oil) can be used as a health food, a food for special medical purposes, or other special foods.

[0078] Example 15: The highly active and highly safe rubber seed oil of the present invention was prepared according to the method in Examples 1-2, and then tablets were prepared according to the following method: Tablets: 100mg of rubber seed oil from this invention Starch as needed Corn syrup as needed Magnesium stearate (appropriate amount) Preparation method: The rubber seed oil of the present invention is mixed with the additives, sieved, and uniformly mixed in a suitable container. The resulting mixture is then granulated and compressed into tablets.

[0079] Example 16: The highly active and safe rubber seed oil of the present invention was prepared according to the method in Examples 1-2. This portion was then further prepared into soft capsules according to the following method: Soft capsules: 1000mg of rubber seed oil from this invention appropriate amount of gelatin Glycerin (appropriate amount) Water appropriate amount Preparation method: Soak gelatin and glycerin in distilled water to allow the gelatin to swell and soften, then stir and mix evenly to obtain a capsule material solution. Take out the prepared capsule material solution and apply it to a flat plate surface to ensure uniform thickness. Then heat it at about 90°C to evaporate the surface moisture, resulting in a soft rubber sheet with a certain degree of toughness and elasticity. Finally, use a pelletizing mold or an automatic rotary capsule forming machine to fill the soft capsule shell with the rubber seed oil (1000mg) of this invention to produce soft capsules.

[0080] Example 17: The highly active and safe rubber seed oil of the present invention was prepared according to the method in Examples 1-2, and then made into powdered oil according to the following method: Powder (Powdered Oil): 10 kg of rubber seed oil of this invention Starch as needed appropriate amount of monoglycerides Corn syrup as needed Preparation method: The rubber seed oil of the present invention is mixed with starch, monoglyceride, corn steep liquor and water, homogenized and emulsified under high pressure, and spray dried to obtain a powder (powdered oil).

[0081] Example 18: Tablets: 10 mg of rubber seed oil, 180 mg of lactose, 55 mg of starch, and 5 mg of magnesium stearate of the present invention were prepared according to the method in Examples 1-2. Preparation method: The rubber seed oil of the present invention prepared according to the method in Examples 1-2, lactose and starch are mixed, and the mixture is moistened with water. The moistened mixture is sieved and dried, then sieved again. Magnesium stearate is added, and the mixture is then compressed into tablets, each weighing 250 mg. The rubber seed oil content of the present invention is 10 mg.

[0082] Example 19: Capsules: 2000g of the highly active and safe rubber seed oil of the present invention, 1000g of gelatin, 500g of glycerin, and 1000g of water were prepared according to the methods in Examples 1-2. Preparation method: Soak 1000g of gelatin and 500g of glycerin in 1000g of water to allow the gelatin to swell and soften. Then, stir and mix evenly to obtain a capsule material solution. Take out the prepared capsule material solution and spread it on a flat plate surface to ensure uniform thickness. Then, heat it at about 90℃ to evaporate the surface moisture, resulting in a soft rubber sheet with certain toughness and elasticity. Finally, use a pelleting mold or an automatic rotary capsule forming machine to fill 1000mg of the rubber seed oil of this invention into the soft capsule shell to obtain soft capsules. Each soft capsule weighs 1.4g, and the rubber seed oil content of this invention is 1000mg.

[0083] Example 20: Powder (powdered oil): 20 kg of the highly active and safe rubber seed oil of the present invention, 10 kg of starch, 2 kg of monoglyceride, 1.2 kg of lecithin, and 35 kg of water were prepared according to the method in Examples 1-2. Preparation method: Accurately weigh 20 kg of rubber seed oil, 10 kg of starch, 2 kg of monoglyceride, and 1.2 kg of lecithin according to the methods in Examples 1-2, and add 35 kg of water. High-pressure homogenization and emulsification are performed to obtain an emulsion, which is then sterilized at 121°C and vacuum spray-dried to obtain a powder (powdered oil). Each 10 g of powder (powdered oil) contains 6.0 g of rubber seed oil.

Claims

1. A health food product, characterized in that, Composed of highly active and safe refined rubber seed oil and a carrier commonly used in health foods, the refined rubber seed oil is prepared by the following method: using crude rubber seed oil as raw material, it is obtained by adding alkali for neutralization, natural sedimentation, centrifugal separation, adsorption filtration, and deodorization. In the neutralization step, the alkali used is food-grade alkali, and the amount of alkali added is equal to the molar mass of the fatty acids contained in the crude oil. The alkali is added in an aqueous solution and then mixed. In the natural sedimentation step, the mixture is allowed to stand at a temperature above 70°C until the fatty acid salt and oil phases are clearly separated. The lower fatty acid salt phase is then discarded, and the oil phase is retained. In the centrifugal separation step, the oil phase temperature is above 70°C and the centrifugal force is greater than or equal to 5000g.

2. The food product as described in claim 1, characterized in that, The centrifugation separation step is carried out using a disc centrifuge or a tubular centrifuge. The centrifugation separation temperature of the oil phase is 70-100℃, the centrifugal force is 5000-30000g, the fatty acid salts are discarded, and the oil phase is separated.

3. The food product as described in claim 2, characterized in that, The centrifugal force is preferably 6000-18000g.

4. The application of the food product as described in any one of claims 1-3, characterized in that, The health food products are intended to improve the health status of people with atherosclerosis.

5. The application of the food as described in claim 4, characterized in that, The highly active and safe refined rubber seed oil is the only active ingredient in health food products.

6. A food for special medical purposes, characterized in that, It is composed of highly active and highly safe refined rubber seed oil and a carrier commonly used in special medical purpose formula foods. The refined rubber seed oil is prepared by the following method: using crude rubber seed oil as raw material, it is obtained by adding alkali for neutralization, natural sedimentation, centrifugation, adsorption filtration, and deodorization. In the neutralization step, the alkali used is food-grade alkali, and the amount of alkali added is equal to the molar mass of the fatty acids contained in the crude oil. The alkali is added in an aqueous solution and then mixed. In the natural sedimentation step, the mixture is allowed to stand at a temperature above 70°C until the fatty acid salt and oil phases are clearly separated. The lower fatty acid salt phase is then discarded, and the oil phase is retained. In the centrifugal separation step, the oil phase temperature is above 70°C and the centrifugal force is greater than or equal to 5000g.

7. The food product as described in claim 6, characterized in that, The centrifugation separation step is carried out using a disc centrifuge or a tubular centrifuge. The centrifugation separation temperature of the oil phase is 70-100℃, the centrifugal force is 5000-30000g, the fatty acid salts are discarded, and the oil phase is separated.

8. The food product as described in claim 7, characterized in that, The centrifugal force is preferably 6000-18000g.

9. The application of the food product as described in any one of claims 6-8, characterized in that, The food is intended to improve the health of people with atherosclerosis.

10. The application of the food as described in claim 9, characterized in that, The highly active and safe refined rubber seed oil is the only active ingredient in food.

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