A laminarin polysaccharide composition with bacteriostatic efficacy and a preparation method and application thereof

Abstract

The application belongs to the technical field of cosmetics, and discloses a fucoidan polysaccharide composition with bacteriostatic efficacy, and a preparation method and application thereof. The fucoidan polysaccharide composition comprises phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, and the mass ratio of the phaeophycus triquetrum fucoidan, the small new moon rhombus fucoidan and the false micro-halochain fucoidan is (1-2):(1-2):(1-2). The fucoidan polysaccharide composition is prepared by compounding specific phaeophycus triquetrum fucoidan, small new moon rhombus fucoidan and false micro-halochain fucoidan, has a synergistic effect, and has excellent antibacterial efficacy. When the fucoidan polysaccharide composition is applied to cosmetics, it has good formula adaptability, does not affect the exertion of efficacy and the stability of the cosmetics.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, and specifically relates to a diatomaceous kelp polysaccharide composition with antibacterial effects, its preparation method, and its application. Background Technology

[0002] Today, consumers' demand for skincare products has gradually shifted from basic cleansing and moisturizing to a pursuit of natural, safe, effective, and specific products. Against this backdrop, naturally derived polysaccharides, such as seaweed polysaccharides, are highly favored due to their excellent biocompatibility, biodegradability, low toxicity, and diverse bioactivities.

[0003] Diatoms, as a diverse and rapidly reproducing group of single-celled microalgae, possess polysaccharide products with unique sulfation modification patterns and monosaccharide compositions, exhibiting potential advantages in antibacterial activity compared to polysaccharides from macroalgae. However, current technologies primarily focus on polysaccharides derived from macroalgae such as brown, red, and green algae (e.g., fucoidan, alginate, carrageenan, agar), while the systematic development and activity research of diatom-derived polysaccharides lags behind. Current research on diatom polysaccharides mainly concentrates on optimizing extraction processes and exploring antiviral and antitumor activities, with very few reports on their application in the field of antibacterial cosmetics. The antibacterial potential of diatom polysaccharides has not yet been fully explored and utilized. Summary of the Invention

[0004] The present invention aims to at least solve one of the technical problems existing in the prior art. To this end, the present invention proposes a diatomaceous laminarin composition, its preparation method, and its application. The diatomaceous laminarin composition of the present invention has excellent antibacterial effects. When applied to cosmetics, it has good formulation compatibility and does not affect its efficacy or the stability of the cosmetics.

[0005] In a first aspect, the present invention provides a diatom laminarin composition comprising laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhomboidea microphylla*, and laminarin from *Pseudomicrocystis*, wherein the mass ratio of the laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhomboidea microphylla*, and laminarin from *Pseudomicrocystis* is (1-2):(1-2):(1-2).

[0006] Specifically, this invention selects laminarin from three specific diatom sources—*Phaeodactylum tricornutum*, *Nyctaginea microcarpa*, and *Pseudomicrocystis*—and blends them in a specific ratio. Compared to laminarin from macroalgae, diatom-derived laminarin exhibits stronger species specificity in both structure and function. This invention is based on the significant differences among *Phaeodactylum tricornutum*, *Nyctaginea microcarpa*, and *Pseudomicrocystis* laminarin in terms of sulfate content, substitution positions, monosaccharide composition, glycosidic bond type, and molecular weight. These structural differences determine their different interaction patterns and affinities with bacterial surface structures. By rationally combining these three diatom laminarins with complementary structural characteristics, multi-target effects can be achieved, including disrupting bacterial cell membrane integrity, inhibiting biofilm formation, and regulating bacterial metabolism, thereby producing a significant synergistic effect and significantly enhanced antibacterial ability, far superior to single components, especially showing remarkable inhibitory ability against *Staphylococcus aureus*.

[0007] In some embodiments of the present invention, the mass ratio of the polysaccharide from *Phaeodactylum tricornutum*, the polysaccharide from *Rhizoctonia solani*, and the polysaccharide from *Pseudomicrocystis* is (1-2):1:(1-2). Preferably, this ratio of the three diatomaceous laminarin polysaccharides produces a better synergistic effect.

[0008] In some embodiments of the present invention, the mass ratio of *Phaeodactylum tricornutum* laminarin, *Rhizoctonia solani* laminarin, and *Pseudomicrocystis* laminarin is 2:1:2. Preferably, this ratio of the three diatomaceous laminarins produces the best synergistic effect.

[0009] A second aspect of the present invention provides a method for preparing the diatomaceous kelp polysaccharide composition described in the first aspect of the present invention, comprising the following steps: The polysaccharides of *Phaeodactylum triangularis*, *Rhomboidella latae*, and *Pseudomicrocystis* were mixed to obtain the diatom laminarin composition.

[0010] In some embodiments of the present invention, diatom laminarin (Phaeodactylum tricornutum laminarin, Rhomboidella pulvinata laminarin, or Pseudomicroscopica laminarin) is prepared by the following method, the specific steps of which are as follows: Mix diatomaceous earth powder with sulfuric acid solution, extract in a water bath, and collect the supernatant by centrifugation. Mix the supernatant with an ethanol-water solution, allow it to precipitate overnight at a low temperature (2-8℃), collect the precipitate by centrifugation, and freeze-dry it. Proteins were removed from the freeze-dried extract, which was then dialyzed in water and freeze-dried again to obtain crude diatomaceous laminarin polysaccharide.

[0011] In some embodiments of the present invention, the preparation process of diatomaceous kelp polysaccharide further includes the following purification steps: The crude diatomaceous kelp polysaccharide was prepared into a solution and loaded onto a DEAE-52 cellulose column (2×30cm). It was then eluted sequentially with 0.1, 0.3 and 0.5M sodium chloride solutions. The eluent was collected, and the polysaccharide fractions obtained by elution with 0.1M sodium chloride solution were combined. After dialyzing and concentration, the fractions were loaded onto a Sephadex G-200 gel chromatography column for elution. The polysaccharide fractions were collected, dialyzed with water, concentrated by gas flow, and then freeze-dried to obtain a light white powder, which yielded refined diatomaceous kelp polysaccharide.

[0012] A third aspect of the present invention provides the use of the diatomaceous earth polysaccharide composition described in the first aspect of the present invention in the preparation of cosmetics with antibacterial effects.

[0013] In some embodiments of the present invention, the cosmetic includes serum, lotion, cream, or mask.

[0014] A fourth aspect of the present invention provides the application of the diatomaceous kelp polysaccharide composition described in the first aspect of the present invention in the preparation of an antibacterial agent, wherein the antibacterial effect is the inhibition of Staphylococcus aureus.

[0015] In a fifth aspect, the present invention provides a cosmetic product comprising the diatomaceous earth polysaccharide composition described in the first aspect of the present invention and cosmetically acceptable excipients.

[0016] In some embodiments of the present invention, the concentration of the diatomaceous earth polysaccharide composition in the cosmetic is 1-10 mg / mL. The concentration can be any one of 1 mg / mL, 2 mg / mL, 4 mg / mL, 6 mg / mL, 8 mg / mL, or 10 mg / mL, or a range formed by any two values. The diatomaceous earth polysaccharide composition exhibits good activity within this applicable concentration range, and the cosmetic system has stable physicochemical properties.

[0017] In some embodiments of the present invention, the concentration of the diatomaceous earth polysaccharide composition in the cosmetic is 1-3.2 mg / mL. The concentration can be any one of 1 mg / mL, 1.2 mg / mL, 1.6 mg / mL, 2.4 mg / mL, or 3.2 mg / mL, or a range formed by any two values. Preferably, it is 1.6 mg / mL, at which the diatomaceous earth polysaccharide composition exhibits the best activity and the cosmetic system has more stable physicochemical properties.

[0018] In some embodiments of the present invention, the excipients include at least one of emollients, emulsifiers, thickeners, humectants, chelating agents, and preservatives.

[0019] In some embodiments of the present invention, the diatomaceous earth polysaccharide composition in the cosmetic is used as an antibacterial active ingredient.

[0020] In some embodiments of the present invention, the cosmetic is a facial mask essence, the components of which include the diatomaceous earth polysaccharide composition described in the first aspect of the present invention, lecithin, 1,3-butanediol, glyceryl polyether-26, disodium ethylenediaminetetraacetate, methylparaben, betaine, 1,2-hexanediol and water.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) This invention is the first to systematically combine laminarin from three diatoms: brown finger algae, small crescent algae, and pseudomicroalgae. The above-mentioned diatom species have significant differences from large brown algae in terms of the fine structure, sulfation modification degree and molecular weight distribution of laminarin. However, there is currently a lack of systematic activity data to support them. This invention fills this technical gap and provides new experimental basis for the application of diatom-derived polysaccharides in the field of antibacterial.

[0022] (2) Regarding antibacterial activity, this invention systematically screened in vitro activity and quantitatively evaluated the inhibition zone diameter of different proportions of compound polysaccharides against Staphylococcus aureus using the agar diffusion method. Experimental results showed that the three diatomaceous kelp polysaccharides produced a significant synergistic effect after being compounded in a specific ratio, and the antibacterial activity of the compound system was significantly better than any single component. Based on this, this invention further determined the optimal compound ratio to achieve the best antibacterial effect and verified the stability and activity retention of the compound system in the mask essence matrix, confirming that it can maintain or even enhance the original antibacterial efficacy in the mask essence matrix, thus solving the problem of easy activity decay of natural polysaccharides in formulations in existing technologies.

[0023] (3) This invention closely integrates basic research with applied development. Based on the above-mentioned compound ratio of kelp polysaccharides, a complete facial mask essence product was constructed. Through systematic efficacy verification and evaluation, a scientific, rigorous and repeatable experimental path was provided for the development of skin care products with clear antibacterial effects. Attached Figure Description

[0024] Figure 1 Comparison of the antibacterial properties of three single diatomaceous laminarin polysaccharides at different concentrations; Figure 2 A comparison of the antibacterial properties of diatomaceous earth polysaccharide compositions with different compound ratios at a total polysaccharide concentration of 1.6 mg / mL; Figure 3 A comparison chart showing the antibacterial properties of a facial mask essence containing three single diatomaceous kelp polysaccharides at different concentrations; Figure 4This is a comparison chart showing the antibacterial properties of a facial mask essence containing three single diatomaceous laminarin polysaccharides and a facial mask essence containing the diatomaceous laminarin polysaccharide composition of Example 6 at a total polysaccharide concentration of 1.6 mg / mL. Detailed Implementation

[0025] To enable those skilled in the art to more clearly understand the technical solutions described in this invention, the following embodiments are provided for illustration. It should be noted that the following embodiments do not constitute a limitation on the scope of protection claimed by this invention.

[0026] Unless otherwise specified, the raw materials, reagents, and devices used in the embodiments of this invention can be obtained from conventional commercial sources or by existing known methods.

[0027] The preparation method of diatom laminarin (Phaeodactylum tricornutum laminarin, Rhomboidella pulvinata laminarin, and Pseudomicrocystis laminarin) in this invention embodiment includes the following specific steps: 1.0 g of clean, impurity-free frozen diatomaceous earth powder was accurately weighed using an analytical balance and added to 50 mL of 50 mM sulfuric acid solution. Extraction was performed twice in a 60 °C water bath, with each extraction lasting 30 min. The supernatant was then collected by centrifugation, and four volumes of 95% ethanol aqueous solution were added. The mixture was allowed to precipitate overnight at 4 °C. The precipitate was collected by centrifugation again and washed twice each with ethanol and acetone, followed by freeze-drying. The freeze-dried extract was dissolved in deionized water, and a chloroform-n-butanol mixture (4:1, v / v) was added to remove proteins. Finally, the deproteinized solution was dialyzed against deionized water for 48 h, and then freeze-dried to obtain crude polysaccharide. 3 mL (10 g / L) of the kelp crude polysaccharide solution was loaded onto a DEAE-52 cellulose column (2 × 30 cm) and eluted sequentially with 0.1, 0.3, and 0.5 M sodium chloride solutions. The eluent was collected at a flow rate of 0.5 mL / min, with one fraction collected every 5 mL. The polysaccharide components were analyzed using the phenol-sulfuric acid method. The polysaccharide fractions obtained by elution with 0.1 M sodium chloride were combined, dialyzed, concentrated, and then loaded onto a Sephadex G-200 gel chromatography column. Elution was performed at a flow rate of 0.2 mL / min, with one fraction collected every 2 mL. Based on the polysaccharide detection results or a pre-defined elution curve, the corresponding kelp polysaccharide fractions were collected, dialyzed with distilled water for 48 h, concentrated by gas flow, and then freeze-dried to obtain a pale white powder, which is the purified kelp polysaccharide.

[0028] The polysaccharide of *Phaeodactylum triangularis* in this invention is abbreviated as PTL; the polysaccharide of *Rhomboidea microcarpa* is abbreviated as NCL; and the polysaccharide of *Pseudomicrocystis* is abbreviated as TPL.

[0029] Example 1 A diatom laminarin composition is formed by mixing laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhizoctonia solani*, and laminarin from *Pseudomicrocystis* in a mass ratio of 1:1:1.

[0030] Example 2 A diatom laminarin composition is formed by mixing laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhizoctonia solani*, and laminarin from *Pseudomicrocystis* in a mass ratio of 2:1:1.

[0031] Example 3 A diatom laminarin composition is formed by mixing laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhizoctonia solani*, and laminarin from *Pseudomicrocystis* in a mass ratio of 1:2:1.

[0032] Example 4 A diatom laminarin composition is formed by mixing laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhizoctonia solani*, and laminarin from *Pseudomicrocystis* in a mass ratio of 1:1:2.

[0033] Example 5 A diatom laminarin composition is formed by mixing laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhomboidea microphylla*, and laminarin from *Pseudomicrocystis* in a mass ratio of 1:2:2.

[0034] Example 6 A diatom laminarin composition is formed by mixing laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhizoctonia solani*, and laminarin from *Pseudomicrocystis* in a mass ratio of 2:1:2.

[0035] Example 7 A diatom laminarin composition is formed by mixing laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhizoctonia solani*, and laminarin from *Pseudomicrocystis* in a mass ratio of 2:2:1.

[0036] Comparative Example 1 Single diatom laminarin polysaccharide: Phaeodactylum tricornutum laminarin polysaccharide.

[0037] Comparative Example 2 Single diatom laminarin polysaccharide: Small Crescent Rhomboid Laminaria polysaccharide.

[0038] Comparative Example 3 Single diatom laminarin: pseudomicroalgae laminarin polysaccharide.

[0039] Comparative Example 4 A diatom laminarin composition is prepared by mixing laminarin from *Phaeodactylum tricornutum* and laminarin from *Rhomboidea microphylla* in a mass ratio of 1:1.

[0040] Comparative Example 5 A diatom laminarin composition is prepared by mixing laminarin from *Rhizoctonia solani* and laminarin from *Pseudomicrocystis* in a mass ratio of 1:1.

[0041] Comparative Example 6 A diatom laminarin composition is prepared by mixing laminarin from *Phaeodactylum tricornutum* and laminarin from *Pseudomicrodon* in a mass ratio of 1:1.

[0042] Determination of antibacterial properties of diatomaceous kelp polysaccharides The single diatomaceous laminarin or diatomaceous laminarin composition provided in Examples 1-7 and Comparative Examples 1-6 were used as samples.

[0043] Nutrient agar medium was used, poured into plates, and after solidification, the bacterial suspension (OD) was evenly spread. 600 >0.3). Subsequently, wells were punched in the plates, and 15 μL of the sample was added to each well, diluted with deionized water to prepare different concentrations (1.0 mg / mL, 1.2 mg / mL, 1.6 mg / mL, 2.4 mg / mL, 3.2 mg / mL). The plates were then incubated at 37°C for 24 h. A positive control was prepared with 1.6 mg / mL ampicillin; the control group consisted of culture medium without any sample. The antibacterial activity of the samples was evaluated by observing and measuring the diameter of the inhibition zones formed around the wells.

[0044] The results of the antibacterial activity test of diatomaceous kelp polysaccharides are as follows: Figure 1-2 And as shown in Table 1-2.

[0045] Table 1. Diameter of the inhibition zones of three diatomaceous laminarin polysaccharides against Staphylococcus aureus.

[0046] Note: In the table, uppercase letters indicate the differences of the same diatomaceous laminarin polysaccharide at different concentrations, while lowercase letters indicate the differences of different diatomaceous laminarin polysaccharides at the same concentration; the same applies below.

[0047] Table 2. Diameter of the inhibition zone of diatomaceous laminarin polysaccharide compositions with different compounding ratios.

[0048] like Figure 1As shown in Table 1, all three diatomaceous laminarin polysaccharides exhibited significant antibacterial effects against Staphylococcus aureus, and this effect was concentration-dependent, meaning that the antibacterial effect significantly increased with increasing polysaccharide concentration. Table 1 shows that PTL showed the best antibacterial effect at a polysaccharide concentration of 3.2 mg / mL, with an inhibition zone diameter of 2.92 ± 0.11 cm, significantly larger than NCL (2.30 ± 0.08 cm) and TPL (2.62 ± 0.10 cm). Although the activities of all three polysaccharides were lower than the positive control, the experimental results confirmed that they all possessed certain antibacterial capabilities. This antibacterial activity suggests that these three diatomaceous laminarin polysaccharides can indirectly intervene in Staphylococcus aureus-mediated skin inflammatory responses by inhibiting its growth, and have the potential to be developed as anti-inflammatory skincare active ingredients.

[0049] Figure 2 The antibacterial effects of different compound ratios of diatomaceous kelp polysaccharide compositions were further compared with those in Table 2 at a total polysaccharide concentration of 1.6 mg / mL. The PTL:NCL:TPL ratio of 2:1:2 showed the best antibacterial effect, with an inhibition zone diameter of 3.28 ± 0.04 cm.

[0050] Feasibility study of the application of diatomaceous kelp polysaccharides in cosmetics A facial mask essence containing different concentrations of diatomaceous kelp polysaccharide was prepared, and the formula is shown in Table 3.

[0051] Table 3 Mask Essence Formula

[0052] The preparation method for facial mask essence is as follows: According to the formula in Table 3, add the ingredients of component 1 to the container, and then stir the mixture at 80-85℃ to fully dissolve it. After cooling to 70℃, add the ingredients of component 2 and stir. When the temperature drops to 60℃, add the ingredients of component 3 and stir at 45℃. Finally, add the water of component 4 and stir evenly.

[0053] Sensory and physicochemical properties of facial mask essence 1. Appearance test: Observe whether the color is uniform and consistent, whether there is any color difference; whether the texture is delicate and whether there is any roughness; whether the aroma is pure and whether there is any off-odor; and whether there is any dirt.

[0054] 2. Stability Test: At room temperature, take 5g of each of the mask essences listed in Table 3 into 10mL centrifuge tubes and centrifuge at 3000, 4000, 5000, 6000, and 7000 r / min for 15min respectively, and observe the oil-water separation. Take 500μL of each of the mask essences listed in Table 3 into 2mL centrifuge tubes, two of each, and place them in a -10℃ refrigerator and a 40℃ constant temperature incubator for testing. Observe at 4, 12, and 24h. After 24h, take them out and place them at room temperature, and compare them with the samples in the centrifuge tubes stored at room temperature. Observe the appearance of the test samples and whether phenomena such as layering, discoloration, and precipitation occur.

[0055] The sensory and physicochemical test results of the facial mask essence are shown in Tables 4 and 5.

[0056] Table 4 Physical properties of facial mask essence

[0057] Table 5 pH values ​​of facial mask essence

[0058] As shown in Tables 4 and 5, the mask essence exhibited a uniform color with no color difference or impurities; a fine texture without any grainy feel; and no unpleasant fragrance or odor. Its pH ranged from 3.78 to 5.78, meeting the national "Cosmetic Safety Technical Specifications" and skin physiological characteristics. Centrifugation at 3000, 4000, 5000, 6000, and 7000 r / min for 15 min did not result in oil-water separation. After placing the mask essence in a -10℃ refrigerator and a 40℃ constant temperature incubator, observations were conducted at 4h, 12h, and 24h. No oil-water separation, discoloration, or precipitation occurred, consistent with samples stored at room temperature, indicating that the prepared mask essence containing diatomaceous earth polysaccharides possesses good stability.

[0059] Antibacterial properties of facial mask essence The specific experimental method is the same as that used in the above-mentioned determination of the antibacterial properties of diatomaceous kelp polysaccharide.

[0060] The antibacterial performance test results of the facial mask essence are as follows: Figure 3-4 And as shown in Table 6. Among them, Figure 3-4In this study, the culture medium containing mask essence without diatomaceous laminarin was designated "Mask"; the culture medium without any sample was designated "control group"; the culture medium containing ampicillin was designated "positive control group"; the culture medium containing mask essence with different diatomaceous laminarin was designated "Mask+PTL", "Mask+NCL", and "Mask+TPL", respectively; and the culture medium containing mask essence containing the diatomaceous laminarin composition of Example 6 was designated "Mask+PTL:Mask+NCL:Mask+TPL=2:1:2".

[0061] Table 6. Diameter of the antibacterial zone in facial mask essence.

[0062] Figure 3 Table 6 shows the antibacterial activity of three mask systems containing single diatomaceous laminarin (Mask+PTL, Mask+NCL, Mask+TPL) as the concentration (1.0~3.2 mg / mL) increases. Overall, all three mask essences containing diatomaceous laminarin showed concentration-dependent antibacterial activity against Staphylococcus aureus. Compared with the Mask group (1.63±0.06 cm), the diameter of the inhibition zone gradually increased as the polysaccharide concentration increased from 1.0 mg / mL to 3.2 mg / mL. In the range of 1.0~1.2 mg / mL, the antibacterial effect of the PTL-containing mask essence was slightly better than the other two groups. When the concentration increased to 1.6 mg / mL, the diameter of the inhibition zone of the PTL-containing mask essence (2.69±0.02 cm) was significantly higher than that of the TPL-containing (2.55±0.07 cm) and NCL-containing (2.56±0.06 cm). As the concentration further increased, the antibacterial advantage of the PTL-containing mask essence continued to expand, reaching a maximum value of 3.11±0.03cm at 3.2mg / mL, which was significantly higher than that of TPL (2.95±0.09cm) and NCL (2.78±0.03cm).

[0063] Figure 4 The antibacterial effects of three single polysaccharide mask systems and a compound polysaccharide mask system (PTL:NCL:TPL=2:1:2) were further compared at a concentration of 1.6 mg / mL. The results showed that the antibacterial effect of the compound polysaccharide mask system (3.14±0.04 cm) was significantly higher than that of each single polysaccharide mask system, indicating that the compounding had a synergistic effect in enhancing antibacterial activity.

[0064] It is worth noting that, as shown in Tables 1 and 6, compared with the antibacterial results of the single polysaccharide in Table 1, the antibacterial activity of all three polysaccharides was enhanced to varying degrees after being added to the mask essence system. Among them, TPL showed the most significant enhancement in antibacterial activity in the mask system, with the inhibition zone diameter increasing by approximately 12.6% at 3.2 mg / mL compared to the single polysaccharide; the inhibition zone diameter increased by approximately 6.5% in the PTL-containing group and by 20.9% in the NCL-containing group. This result indicates that the mask essence matrix may have a synergistic effect with TPL, PTL, and NCL, further enhancing its antibacterial properties.

[0065] In summary, all three types of diatom laminarin polysaccharides maintained good antibacterial activity in the mask essence system. This invention combines the polysaccharides of *Phaeodactylum tricornutum*, *Rhizoctonia solani*, and *Pseudomicrocystis* as antibacterial functional ingredients added to the mask product, which can effectively endow the product with the ability to inhibit Staphylococcus aureus, providing experimental evidence for the development of natural skin care products with antibacterial effects.

[0066] The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the embodiments described. Those skilled in the art can make various equivalent modifications or substitutions without departing from the spirit of the present invention, and these equivalent modifications or substitutions are all included within the scope defined by the claims of this application.

Claims

1. A diatomaceous laminarin polysaccharide composition, characterized in that, It includes laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhomboidea microphylla*, and laminarin from *Pseudomicrocystis*, wherein the mass ratio of laminarin from *Phaeodactylum tricornutum*, laminarin from *Rhomboidea microphylla*, and laminarin from *Pseudomicrocystis* is (1-2):(1-2):(1-2).

2. The diatomaceous earth polysaccharide composition according to claim 1, characterized in that, The mass ratio of the polysaccharide from *Phaeodactylum tricornutum*, the polysaccharide from *Rhizoctonia solani*, and the polysaccharide from *Streptomyces pseudomicroscopicus* is (1-2):1:(1-2).

3. The diatomaceous earth polysaccharide composition according to claim 2, characterized in that, The mass ratio of the polysaccharides of *Phaeodactylum triangularis*, *Rhomboidella latae*, and *Pseudomicrocystis* was 2:1:

2.

4. The method for preparing the diatomaceous laminarin composition according to any one of claims 1-3, characterized in that, Includes the following steps: The polysaccharides of *Phaeodactylum triangularis*, *Rhomboidella latae*, and *Pseudomicrocystis* were mixed to obtain the diatom laminarin composition.

5. The use of the diatomaceous earth polysaccharide composition according to any one of claims 1-3 in the preparation of cosmetics with antibacterial effects.

6. The use of the diatomaceous laminarin composition according to any one of claims 1-3 in the preparation of an antibacterial agent, characterized in that, The antibacterial effect refers to the inhibition of Staphylococcus aureus.

7. A cosmetic product, characterized in that, The cosmetic comprises the diatomaceous earth polysaccharide composition according to any one of claims 1-3 and cosmetically acceptable excipients.

8. The cosmetic product according to claim 7, characterized in that, The concentration of the diatomaceous earth polysaccharide composition in the cosmetic is 1-10 mg / mL.

9. The cosmetic product according to claim 8, characterized in that, The concentration of the diatomaceous earth polysaccharide composition in the cosmetic is 1-3.2 mg / mL.

10. The cosmetic product according to claim 7, characterized in that, The excipients include at least one of emollients, emulsifiers, thickeners, humectants, chelating agents, and preservatives.

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