Monitoring control device for protein immunoblot gel electrophoresis experiments
By combining color mark sensors and power control components, precise monitoring and timely control of protein blot positions in Western blotting gel electrophoresis experiments are achieved, solving the problems of inaccurate monitoring and untimely power cut-off in existing technologies, and improving experimental efficiency and compatibility.
Patent Information
- Application Number
- CN202610395614.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-30
- Publication Date
- 2026-07-10
AI Technical Summary
Existing Western blotting gel electrophoresis experiments cannot accurately determine the location of protein blots over time, relying on manual operation, which leads to problems such as inaccurate monitoring and untimely power cut-off.
Employing a color mark sensor assembly and a power control assembly, the color mark sensor detects color difference changes during electrophoresis and automatically controls the power supply to achieve accurate identification of protein blot locations and timely power cut-off.
It improves the monitoring accuracy and control efficiency of protein immunoblotting gel electrophoresis experiments, reduces human dependence, ensures the timeliness and accuracy of power cut-off, and is adaptable to various electrophoresis devices and experimental objectives.
Smart Images
Figure CN122361577A_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the technical field of biological experimental instruments, specifically relating to a monitoring and control device for protein immunoblotting gel electrophoresis experiments. Background Technology
[0002] Western blotting is an experimental technique widely used in molecular biology, biochemistry, and immunogenetics to detect the presence, relative molecular weight, and expression level of specific proteins from complex protein mixtures. Western blotting gel electrophoresis is a key step in Western blotting experiments, primarily using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) to separate proteins into different bands based on their molecular weight.
[0003] Currently, for Western blotting gel electrophoresis experiments, the existing method for observing the designated protein blot locations in the laboratory typically involves personnel monitoring the process and visually assessing whether the blot has been reached before manually cutting off the power to the electrophoresis apparatus (electrophoresis unit). It is evident that the existing control methods for Western blotting gel electrophoresis have the following drawbacks: they can only control the electrophoresis process through timing, but cannot precisely determine the location of the protein blot over time; this reliance on manual operation increases monitoring stress and leads to inaccurate monitoring; furthermore, the timely and accurate cutting off of the electrophoresis power supply cannot be guaranteed. Summary of the Invention
[0004] This invention aims to provide a monitoring and control device for protein immunoblotting gel electrophoresis experiments, which can improve the monitoring and control efficiency of protein immunoblotting gel electrophoresis experiments. This invention is achieved through the following technical solutions:
[0005] A monitoring and control device for a protein immunoblotting gel electrophoresis experiment, comprising:
[0006] Box;
[0007] A color mark sensor assembly, including a color mark sensor disposed on the front side of the exterior of the enclosure;
[0008] The power control component includes a power input interface and a power output interface disposed on the enclosure, and a power on / off control module disposed inside the enclosure; the power on / off control module is electrically connected to the color mark sensor and controls the on / off of the power input interface and the power output interface according to the sensing signal of the color mark sensor.
[0009] As a preferred technical solution, the color mark sensor is fixedly installed on the front side of the outside of the box, and the box has height-adjustable support feet.
[0010] As a preferred technical solution, the color mark sensor is adjustable in height relative to the front of the enclosure.
[0011] As a preferred technical solution, the power on / off control module includes an air switch, a switching power supply, a relay, and a contactor; the power input interface is used to connect to mains power, the input terminal of the air switch is connected to the power input interface, and the output terminal of the air switch is connected to the input terminal of the contactor; the output terminal of the contactor is connected to the power output interface, which is used to connect to the electrophoresis device; the signal trigger terminal of the switching power supply is connected to the sensing signal of the color mark sensor, the control terminal of the switching power supply controls the on / off state of the relay, and the on / off state of the relay controls the opening and closing of the contactor.
[0012] As a preferred technical solution, the color mark sensor assembly further includes a screw, a screw bracket, a guide rod, a sensor bracket, and a driving mechanism; the screw bracket is fixedly disposed on the front side of the outer side of the housing, and the screw is arranged vertically on the screw bracket; the guide rod is fixed parallel to one side of the screw; the sensor bracket includes a sensor assembly part, a screw sleeve part, and a guide sleeve part; the color mark sensor is mounted on the sensor assembly part with the detection direction facing forward; the screw sleeve part is screwed onto and sleeved on the screw; the guide sleeve part is slidably sleeved on the guide rod; the driving mechanism drives the screw to rotate relative to the screw bracket.
[0013] As a preferred technical solution, the screw support includes an upper support and a lower support arranged at intervals, the upper end of the screw passes through the upper support and is connected to the drive mechanism, and the lower end of the screw is supported on the lower support.
[0014] As a preferred technical solution, the driving mechanism is a driving handwheel, which is mounted on the upper bracket via a handwheel bracket and is connected to the screw drive.
[0015] As a preferred technical solution, the driving mechanism is a stepper motor, which is mounted on the upper support via a motor bracket and is connected to the screw drive; the monitoring and control device for the protein immunoblotting gel electrophoresis experiment also includes a motor control module, which is electrically connected to the stepper motor.
[0016] As a preferred technical solution, the guide rod is fixedly disposed between the upper bracket and the lower bracket and located on the rear side of the screw, and the assembly part, screw sleeve part and guide sleeve part of the sensor bracket are arranged sequentially front and back.
[0017] As a preferred technical solution, the power control component also includes an indicator light, which is connected to the switching power supply control.
[0018] The monitoring and control device for protein immunoblotting gel electrophoresis provided by this invention uses a color mark sensor to determine color difference changes during the electrophoresis experiment, enabling easier and more accurate identification of the blot location with high detection precision, eliminating reliance on manual identification. Furthermore, after identification, the power supply to the electrophoresis device can be promptly and automatically cut off using a power control component. In addition, the highly adjustable structural design allows for height-adjustable monitoring positions of the color mark sensor, adapting to various electrophoresis devices or multiple experimental targets, thus improving compatibility. Attached Figure Description
[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0020] Figure 1 This is a three-dimensional view of the monitoring and control device for protein immunoblotting gel electrophoresis provided in an embodiment of the present invention.
[0021] Figure 2 This is a right-side view of the monitoring and control device for protein immunoblotting gel electrophoresis provided in an embodiment of the present invention.
[0022] Figure 3 This is a left perspective view of the monitoring and control device for protein immunoblotting gel electrophoresis provided in this embodiment of the invention.
[0023] Figure 4 This is a rear view of the monitoring and control device for protein immunoblotting gel electrophoresis provided in an embodiment of the present invention.
[0024] Figure 5 yes Figure 1 The block diagram of the electrical control principle of the monitoring and control device for protein immunoblotting gel electrophoresis provided in the embodiments of the present invention in Part A is shown. Detailed Implementation
[0025] To make the technical solution of the present invention clearer and its technical advantages more apparent, the technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of the present invention.
[0026] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus.
[0027] In the description of this invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "clockwise", "counterclockwise", "front", "back", etc., indicating the orientation or positional relationship, are all based on the orientation or relative positional relationship shown in the accompanying drawings, and are intended to facilitate a clear description of the structure of the product or device, and are not intended to limit the actual orientation of the product or device during production, use, sales, etc.
[0028] Furthermore, the terms "first" and "second" are used only for distinguishing purposes in the description and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, unless otherwise stated, "a plurality of" means two or more, unless otherwise expressly defined. The technical solutions of the embodiments of this invention will be explained and described below with reference to the accompanying drawings, but the following embodiments are only preferred embodiments of this invention and not all of them.
[0029] Combination Figures 1 to 4 As shown, a basic embodiment of the present invention provides a monitoring and control device for a protein immunoblotting gel electrophoresis experiment, including a housing 10, a color mark sensor assembly 20, and a power control assembly. The color mark sensor assembly 20 includes a color mark sensor 21 disposed on the front side of the housing 10; the power control assembly includes a power input interface 31 and a power output interface 32 disposed on the housing 10, and also includes a power on / off control module disposed inside the housing 10; the power on / off control module is electrically connected to the color mark sensor 21 and controls the on / off state of the power input interface 31 and the power output interface 32 according to the sensing signal of the color mark sensor 21.
[0030] See also Figures 1 to 4 To make the height of the color mark sensor 21 adjustable, as an optional optimized embodiment, the color mark sensor 21 is disposed on the front side of the outer side of the housing 10 with adjustable height relative to the housing 10. Of course, as another optional optimized embodiment, the color mark sensor 21 is fixedly disposed on the front side of the outer side of the housing 10, and the housing 10 has height-adjustable support feet 11.
[0031] See also Figure 3 and Figure 5 As an optional specific embodiment, the power on / off control module includes an air switch 331, a switching power supply 332, a relay 333, and a contactor 334. A power input interface 31 is used to connect to mains power. The input terminal of the air switch 331 is connected to the power input interface 31, and the output terminal of the air switch 331 is connected to the input terminal of the contactor 334. The output terminal of the contactor 334 is connected to a power output interface 32, which is used to connect to the electrophoresis apparatus. The signal trigger terminal of the switching power supply 332 is connected to the sensing signal of the color mark sensor 21. The control terminal of the switching power supply 332 controls the on / off state of the relay 333, which in turn controls the opening and closing of the contactor 334. When the color mark sensor 21 senses the mark of the electrophoresis experiment, the switching power supply 332 controls the relay 333 to open, and the relay 333 then controls the contactor 334 to disconnect the power supply, cutting off the power supply to the electrophoresis apparatus.
[0032] See also Figures 1 to 4 As an optional specific embodiment, the color mark sensor assembly 20 further includes a screw 22, a screw bracket 23, a guide rod 24, a sensor bracket 25, and a drive mechanism 26. The screw bracket 23 is fixedly disposed on the front side of the outer side of the housing 10, and the screw 22 is disposed vertically on the screw bracket 23. The guide rod 24 is fixed parallel to one side of the screw 22. The sensor bracket 25 includes a sensor assembly part 251, a screw sleeve part 252, and a guide sleeve part 253. The color mark sensor 21 is mounted on the sensor assembly part 251 with the detection direction facing forward. More specifically, there can be two color mark sensors 21, which are mounted on the sensor assembly part 251 with a left-right interval. The screw sleeve part 252 is screwed and sleeved on the screw 22, and the guide sleeve part 253 is slidably sleeved on the guide rod 24. The drive mechanism 26 drives the screw 22 to rotate relative to the screw bracket 23, thereby driving the sensor bracket 25 and the color mark sensor 21 disposed on it to move up and down (i.e., vertically adjustable).
[0033] See Figure 2 and Figure 3 As shown in the figure, in a more specific embodiment, the screw support 23 includes an upper support 231 and a lower support 232 that are spaced apart vertically. The upper end of the screw 22 passes through the upper support 231 and is connected to the drive mechanism 26, and the lower end of the screw 22 is supported on the lower support 232.
[0034] See also Figures 1 to 4As an optional specific embodiment, the drive mechanism 26 is a drive handwheel, which is mounted on the upper bracket 231 via a handwheel bracket 27 and is drivenly connected to the screw 22. Regarding the drive mechanism, as an alternative embodiment, the drive mechanism is a stepper motor, which is mounted on the upper bracket 231 via a motor bracket and is drivenly connected to the screw 22. Based on this alternative embodiment, the monitoring and control device 100 for the protein immunoblotting gel electrophoresis experiment further includes a motor control module, which is electrically connected to the stepper motor.
[0035] In addition, the power control component also includes an indicator light 39, which is electrically connected to the switching power supply 332; when the color mark sensor 21 detects the mark of the electrophoresis experiment, the switching power supply 332 also synchronously controls the indicator light 39 to flash.
[0036] See also Figures 1 to 4 As a more specific embodiment, the guide rod 24 is fixedly disposed between the upper bracket 231 and the lower bracket 232 and is located on the rear side of the screw 22. The assembly part 251, the screw sleeve part 252 and the guide sleeve part 253 of the sensor bracket 25 are arranged in sequence.
[0037] See also Figures 1 to 4 As a more specific embodiment, the guide rod 24 is provided with height markings. In addition, for easy carrying, a handle 12 is provided at the top of the case 10.
[0038] The monitoring and control device 100 for protein immunoblotting gel electrophoresis provided in the above embodiment uses a color mark sensor 21 to determine color difference changes during the electrophoresis experiment. This allows for easier and more accurate identification of the blot location, resulting in high detection precision and eliminating reliance on manual identification. Furthermore, after identification, the power supply to the electrophoresis device can be promptly and automatically cut off using a power control component. In addition, the height-adjustable structural design allows for height-adjustable monitoring positions of the color mark sensor 21, adapting to various electrophoresis devices or multiple experimental targets, thus improving compatibility.
[0039] The above description discloses only preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. Therefore, equivalent variations made in accordance with the claims of the present invention are still within the scope of the present invention.
Claims
1. A monitoring and control device for a protein immunoblotting gel electrophoresis experiment, characterized in that, include: Box; A color mark sensor assembly, including a color mark sensor disposed on the front side of the exterior of the enclosure; The power control component includes a power input interface and a power output interface disposed on the enclosure, and a power on / off control module disposed inside the enclosure; the power on / off control module is electrically connected to the color mark sensor and controls the on / off of the power input interface and the power output interface according to the sensing signal of the color mark sensor.
2. The monitoring and control device for protein immunoblotting gel electrophoresis experiment according to claim 1, characterized in that, The power on / off control module includes an air switch, a switching power supply, a relay, and a contactor; the power input interface is used to connect to mains power, the input terminal of the air switch is connected to the power input interface, and the output terminal of the air switch is connected to the input terminal of the contactor; the output terminal of the contactor is connected to the power output interface, and the power output interface is used to connect to the electrophoresis device; The signal trigger terminal of the switching power supply is connected to the sensing signal of the color mark sensor, the control terminal of the switching power supply controls the on / off state of the relay, and the on / off state of the relay controls the opening and closing of the contactor.
3. The monitoring and control device for protein immunoblotting gel electrophoresis according to claim 1 or 2, characterized in that, The color mark sensor is fixedly installed on the front side of the outside of the housing, and the housing has height-adjustable support feet.
4. The monitoring and control device for protein immunoblotting gel electrophoresis according to claim 1 or 2, characterized in that, The color mark sensor is adjustable in height relative to the front of the enclosure.
5. The monitoring and control device for protein immunoblotting gel electrophoresis according to claim 4, characterized in that, The color mark sensor assembly further includes a screw, a screw bracket, a guide rod, a sensor bracket, and a drive mechanism. The screw bracket is fixedly disposed on the front side of the outer side of the housing, and the screw is arranged vertically on the screw bracket. The guide rod is fixed parallel to one side of the screw. The sensor bracket includes a sensor assembly part, a screw sleeve part, and a guide sleeve part. The color mark sensor is mounted on the sensor assembly part with the detection direction facing forward. The screw sleeve part is screwed onto and sleeved on the screw, and the guide sleeve part is slidably sleeved on the guide rod. The drive mechanism drives the screw to rotate relative to the screw bracket.
6. The monitoring and control device for protein immunoblotting gel electrophoresis according to claim 5, characterized in that, The screw support includes an upper support and a lower support arranged at intervals. The upper end of the screw passes through the upper support and is connected to the drive mechanism, while the lower end of the screw is supported on the lower support.
7. The monitoring and control device for protein immunoblotting gel electrophoresis experiment according to claim 6, characterized in that, The driving mechanism is a drive handwheel, which is mounted on the upper bracket via a handwheel bracket and is connected to the screw drive.
8. The monitoring and control device for protein immunoblotting gel electrophoresis experiment according to claim 6, characterized in that, The driving mechanism is a stepper motor, which is mounted on the upper support via a motor bracket and is connected to the screw drive. The monitoring and control device for the protein immunoblotting gel electrophoresis experiment also includes a motor control module, which is electrically connected to the stepper motor.
9. The monitoring and control device for protein immunoblotting gel electrophoresis according to claim 6, characterized in that, The guide rod is fixedly installed between the upper bracket and the lower bracket, and is located on the rear side of the screw. The assembly part, the screw sleeve part and the guide sleeve part of the sensor bracket are arranged in sequence.
10. The monitoring and control device for protein immunoblotting gel electrophoresis experiment according to claim 2, characterized in that, The power control component also includes indicator lights, which are connected to the switching power supply control.