One-step nucleic acid rapid extraction and rapid detection multifunctional reaction assembly and use method thereof

By integrating sample processing, nucleic acid purification, and amplification detection into a closed system, a multifunctional reaction component for rapid nucleic acid extraction and detection has been developed, solving the problems of cumbersome processes and easy contamination in existing technologies, and achieving low-cost and efficient nucleic acid detection.

CN122381903APending Publication Date: 2026-07-14SHENZHEN BM LIFE SCI CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN BM LIFE SCI CO LTD
Filing Date
2026-03-16
Publication Date
2026-07-14

AI Technical Summary

Technical Problem

The existing nucleic acid testing process is cumbersome, relies on complex equipment, and is prone to aerosol pollution, making it difficult to meet the needs of primary healthcare institutions and families for convenience and speed in self-testing.

Method used

A one-step nucleic acid rapid extraction and detection multifunctional reaction component was designed, integrating sample processing, nucleic acid purification and amplification detection into a closed system. The entire process is completed by manual pushing or centrifugation. The modular design and breathable air duct ensure air pressure balance.

Benefits of technology

It has achieved a fully enclosed nucleic acid testing method that is easy to operate, prevents pollution, and is low-cost, making it suitable for primary healthcare institutions and home self-testing, and improving the accuracy and reliability of the test.

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Abstract

This invention discloses a one-step rapid nucleic acid extraction and detection multifunctional reaction assembly and its usage method, belonging to the field of molecular diagnostic technology. The assembly includes a sample loading and driving unit (syringe), a lysis filter tube, a rapid nucleic acid extraction inner tube, a lower plug, and a reaction tube pre-filled with lyophilized reagent balls. The rapid nucleic acid extraction inner tube is placed inside the reaction tube, with a permeable air channel between its outer wall and the inner wall of the reaction tube. All components are sealed together by interference fit or other methods to form a fully enclosed system. In use, the sample is driven by the syringe and flows sequentially through the lysis filter tube and the rapid nucleic acid extraction inner tube, completing lysis, impurity filtration, and adsorption; the elution buffer elutes the nucleic acid to the bottom of the reaction tube, dissolving the lyophilized reagent to form the reaction system, which can then be directly used for detection. This invention highly integrates nucleic acid extraction, purification, and amplification detection into one unit, making operation extremely simple, the entire process completely sealed to effectively prevent contamination, and the cost low, making it particularly suitable for point-of-care testing (POCT) scenarios.
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