A quinone-based functional material derived from cyanobacteria, its preparation method and application

Abstract

A cyanobacterial quinone electrolyte preparation method and application. The present application belongs to the technical field of electrolyte preparation. The quinone compound extracted from cyanobacteria is used as the core, the electrolyte has good redox reversibility, can efficiently mediate electron transfer, significantly improves the electrochemical performance of the electrolyte, and the cyanobacterial quinone compound is derived from natural algae, has the advantages of environmental friendliness, good biocompatibility, easy availability of raw materials and low cost, compared with artificial synthetic quinone materials or other natural quinone extracts, the dispersion stability and electrochemical cycle stability in the electrolyte are more outstanding, thereby the working efficiency and service life of the electrolyte adapter device can be greatly improved.

Description

Technical Field

[0001] This invention belongs to the field of electrolyte preparation, specifically relating to a method for preparing and applying an electrolyte containing quinone compounds derived from cyanobacteria. Background Technology

[0002] In flow battery devices, the electrolyte is a core component, directly determining the cycle stability, energy efficiency, and lifespan of the electrolyte. Quinone compounds possess excellent redox reversibility, enabling them to mediate electron transfer and enhance the electrochemical performance of the electrolyte. Anthraquinone derivatives, in particular, have shown promising application prospects in aqueous organic flow battery and other electrolyte systems. However, quinone compound electrolytes still have limitations at present.

[0003] Firstly, most of the quinone additives used in electrolytes are artificially synthesized, which has problems such as complex preparation processes, high costs, and insufficient environmental friendliness. In addition, some artificially synthesized quinone compounds have defects such as poor stability and fast decay rate. These problems make quinone-containing electrolytes expensive and difficult to use on a large scale.

[0004] Meanwhile, cyanobacteria, as one of the most widely distributed and fastest-reproducing microbial resources on Earth, have advantages such as short growth cycle, low cultivation cost, and strong environmental adaptability. Their cells are rich in a variety of natural quinone compounds (such as ubiquinone, plastid quinone, naphthoquinone, etc.). These natural quinone compounds not only have redox activity similar to artificially synthesized quinones, but also have unique advantages such as good biocompatibility, environmental friendliness, and non-degradability, making them ideal natural raw materials to replace artificially synthesized quinone additives.

[0005] Therefore, using quinone compounds extracted from cyanobacteria to prepare electrolytes for flow batteries can not only reduce the cost of quinone electrolytes, but also treat waste as waste, making it environmentally friendly. However, current methods for preparing cyanobacterial quinone compounds suffer from problems such as cumbersome processes, low extraction efficiency, and insufficient product purity, making large-scale preparation difficult. Furthermore, the extracted cyanobacterial quinone compounds have not been applied to electrolytes, failing to fully utilize the advantages of natural quinone compounds.

[0006] Therefore, developing a method for preparing electrolytes using quinone compounds derived from cyanobacteria to solve the current problems with quinone compound electrolytes is of practical significance and value. Summary of the Invention To address the shortcomings and deficiencies of the existing technology, this invention provides a method for preparing an electrolyte containing cyanobacterial quinone compounds and its application.

[0007] The technical solution of the present invention is as follows: One objective of this invention is to provide a method for preparing an electrolyte containing cyanobacterial quinone compounds, wherein the method comprises the following steps: S1: Pretreatment operations such as extraction, centrifugation and evaporation concentration of cyanobacteria; S2: Prepare a buffer solution using KH2PO4 and Na2HPO4, then add Na2SO4 and a pretreated cyanobacterial quinone compound solution, and finally dilute with deionized water to prepare an electrolyte solution. To further specify, the S1 process is as follows: mix cyanobacteria powder with 95% ethanol and stir.

[0008] Furthermore, the concentration of ethanol in the mixed solution is 65%–95%, and the ratio of the mass of cyanobacterial powder to the volume of ethanol in the mixed solution is 1:10 (g:ml).

[0009] Furthermore, the extraction temperature is room temperature, the stirring time is 1h to 3h, and the stirring speed is 300 to 500 r / min.

[0010] Furthermore, the centrifuge speed is specified as 5000–10000 r / min, and the centrifugation time is specified as 5–15 min.

[0011] To further specify, after centrifugation in S1, the supernatant of the solid-liquid mixture should be collected using a pipette or dropper.

[0012] Furthermore, the process of evaporation and concentration is further specified by continuously heating at 30°C to 40°C.

[0013] To further refine the design, the distillation flask is connected to a U-shaped tube filled with half water to simulate a vacuum environment.

[0014] Furthermore, after evaporation and concentration, the product volume is reduced to one-tenth of its original size.

[0015] Further specified, the concentration of KH2PO4 in the electrolyte prepared by S2 is 0.01mol / L to 0.1mol / L.

[0016] Furthermore, the concentration of Na2HPO4 in the electrolyte is specified to be 0.01 mol / L to 0.1 mol / L.

[0017] Furthermore, the concentration of Na2SO4 in the electrolyte is specified to be 0.05 mol / L to 0.5 mol / L.

[0018] Furthermore, the concentration of cyanobacterial quinone extract in the electrolyte is specified to be 1%–2%.

[0019] Furthermore, the pH of the electrolyte is specified to be 6.8 ± 0.5.

[0020] The second objective of this invention is to provide an electrolyte of cyanobacterial quinone compounds prepared by the above method, wherein the cyanobacterial quinone compound electrolyte is prepared by quinone compounds extracted from cyanobacteria, KH2PO4, Na2HPO4, and Na2SO4.

[0021] A third objective of this invention is to provide an application of the cyanobacterial quinone compound electrolyte prepared by the above method in a flow battery.

[0022] The advantages of this invention compared to the prior art are: (1) The preparation method of the present invention is simple and convenient to operate. The pretreatment of cyanobacteria can be completed in just three steps: extraction, centrifugation and evaporation concentration. No complicated purification equipment is required, which reduces the preparation cost and is suitable for large-scale production. The evaporation concentration adopts a 40°C vacuum environment, which can avoid the degradation of quinone compounds due to high temperature oxidation and ensure the activity and purity of the product.

[0023] (2) This invention uses cyanobacteria as raw material, which is widely available, inexpensive and renewable. Compared with artificially synthesized quinone compounds, it greatly reduces the preparation cost, and is environmentally friendly and biocompatible, which is in line with the concept of green development.

[0024] (3) The cyanobacterial quinone compound electrolyte prepared by the present invention can efficiently mediate electron transfer, significantly reduce the capacity decay rate of the electrolyte, improve the cycle stability and working efficiency of the electrolytic cell, and solve the defects of poor stability and high cost of existing artificially synthesized quinone compound electrolytes. Attached Figure Description

[0025] Figure 1 This is a diagram of the cyanobacteria pretreatment process; Figure 2 This is a flowchart of the preparation process of cyanobacterial quinone compound electrolyte; Figure 3 The image shows a physical picture of the cyanobacterial quinone compound electrolyte prepared according to the above method (the electrolyte is on the left, and the quinone compound is on the right). Figure 4 It is a voltage-current comparison graph of electrolytes prepared according to experimental examples and comparative examples (experimental group corresponds to the example, control group corresponds to the comparative example). Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0027] Unless otherwise specified, the experimental methods used in the following examples are conventional methods. Unless otherwise specified, the materials, reagents, methods, and instruments used are all conventional materials, reagents, methods, and instruments in the art, and can be obtained commercially by those skilled in the art.

[0028] The terms “comprising,” “including,” “having,” “containing,” or any other variation thereof, as used in the following embodiments, are intended to cover non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that includes the listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or elements inherent to such a composition, step, method, article, or apparatus.

[0029] When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or a range defined by a series of upper and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pair of any upper or preferred value with any lower or preferred value, regardless of whether the range is disclosed individually. For example, when the range “300 to 500 rpm” is disclosed, the described range should be interpreted as including the ranges “300 to 400 rpm,” “400 to 500 rpm,” “300 to 450 rpm,” etc. When numerical ranges are described herein, unless otherwise stated, the range is intended to include its endpoints and all integers and fractions within that range. In this specification and claims, range definitions may be combined and / or interchanged, unless otherwise stated, these ranges include all subranges contained therein.

[0030] The indefinite articles “a” and “an” preceding an element or component of this invention do not impose any limitation on the quantity (i.e., number of occurrences) of the element or component. Therefore, “an” or “a” should be interpreted as including one or at least one, and the singular form of an element or component also includes the plural form, unless the quantity clearly refers only to the singular form.

[0031] Example: The preparation method of the cyanobacterial quinone compound electrolyte in this embodiment is carried out according to the following steps: Step 1: Pretreatment operations including cyanobacteria extraction, centrifugation, and evaporation concentration. First, take dried cyanobacteria powder and add it to 95% ethanol at a ratio of 1:10 (g:ml) between the mass of the cyanobacteria powder and the volume of 95% ethanol. Place the mixture at room temperature and magnetically stir at 400 r / min for 2 hours to obtain an extract mixture of cyanobacteria quinone compounds.

[0032] The resulting extraction mixture was then poured into a centrifuge, the centrifuge speed was adjusted to 8000 r / min, and centrifuged for 10 min. After centrifugation, the supernatant was collected, the precipitate was removed, and the crude extract of cyanobacterial quinone compounds was obtained.

[0033] Finally, the crude extract was placed in an evaporator, the ambient temperature was set to 40℃ and the environment was under vacuum, and the extract was concentrated by rotation until the volume of the crude extract was reduced to one-tenth of its original volume. Concentration was then stopped to obtain a pretreated solution of cyanobacterial quinone compounds.

[0034] Step 2: Prepare the electrolyte solution containing quinone compounds derived from cyanobacteria Weigh 13.6 g KH2PO4, 17.9 g Na2HPO4·12H2O, and 28.4 g Na2SO4. Take 10 ml of the pretreated cyanobacterial quinone compound solution and dilute it to 1 L with deionized water to prepare the cyanobacterial quinone compound electrolyte.

[0035] Comparative example: The electrolyte preparation method for this comparative example is as follows: Weigh 13.6 g KH2PO4, 17.9 g Na2HPO4·12H2O, and 28.4 g Na2SO4, and dilute to 1 L with deionized water to prepare an electrolyte free of cyanobacterial quinone compounds.

[0036] The above description is merely a preferred embodiment of the present invention. These specific embodiments are different implementations based on the overall concept of the present invention, and the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. An electrolyte containing quinone compounds derived from cyanobacteria and its preparation method, characterized in that, Follow these steps: S1: Pretreatment operations such as extraction, centrifugation and evaporation concentration of cyanobacteria; S2: Prepare a buffer solution using KH2PO4 and Na2HPO4, then add Na2SO4 and a pretreated cyanobacterial quinone compound solution, and finally dilute with deionized water to prepare an electrolyte solution.

2. The method according to claim 1, characterized in that, The specific process of S1 is as follows: mix cyanobacteria powder with ethanol, stir the mixed solution at room temperature, then centrifuge to collect the supernatant, and then vacuum evaporate and concentrate the supernatant.

3. The method according to claim 2, characterized in that, When cyanobacteria are mixed with ethanol, the ethanol concentration in the mixed solution is 65% to 95%, and the mass ratio of cyanobacteria powder to ethanol volume in the mixed solvent is 1:10 (g:ml).

4. The method according to claim 2, characterized in that, During stirring, the mixed solution is stirred at room temperature for 1 to 3 hours at a speed of 300-500 r / min.

5. The method according to claim 2, characterized in that, During the S1 centrifugation process, the rotation speed is 8000 r / min and the centrifugation time is 5 to 15 min.

6. The method according to claim 2, characterized in that, After centrifugation in S1, use a pipette to collect the supernatant of the solid-liquid mixture.

7. The method according to claim 1, characterized in that, The electrolyte parameters prepared by S2 are as follows: KH2PO4 concentration is 0.1 mol / L, Na2HPO4 concentration is 0.05 mol / L, Na2SO4 concentration is 0.2 mol / L, cyanobacterial quinone extract concentration is 1%–2%, and pH is 6.8 ± 0.

2.

8. The cyanobacterial quinone compound electrolyte prepared by the method according to any one of claims 1-7, wherein the cyanobacterial quinone compound electrolyte is an electrolyte prepared from quinone compounds extracted from cyanobacteria, KH2PO4, Na2HPO4, and Na2SO4.

9. The application of the cyanobacterial quinone compound electrolyte prepared by the method of any one of claims 1-8 in carbon fixation in flow batteries.

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