Methods for inducing oocyte-like bodies in garlic and methods for oocyte-like body-mediated genetic transformation
By inducing transparent, sticky callus and FELB from garlic root explants under specific culture and light conditions, the lack of a garlic-based frog egg induction and genetic transformation system was solved, achieving efficient regeneration and genetic transformation of garlic, and improving garlic's reproductive efficiency and disease and pest resistance.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ZHOUKOU NORMAL UNIV
- Filing Date
- 2026-03-05
- Publication Date
- 2026-07-17
AI Technical Summary
There are currently no systematic reports on the induction of FELB in garlic frog eggs and FELB-mediated genetic transformation systems, which limits the advancement of basic research and production applications of garlic and other lily family plants.
Using a specific culture medium formulation, garlic root explants were induced to produce transparent, sticky callus and FELB. Genetic transformation was carried out through FELB-mediated methods, including the use of plant growth regulators such as MS salt, MS organic, NAA, 2,4-D, and 6-BA. Combined with light and dark conditions, element starvation and carbon source starvation were designed to establish a garlic FELB-mediated regeneration and genetic transformation system.
The method efficiently induced FELB in garlic, successfully achieved the transformation of exogenous genes, established a stable regeneration and genetic transformation system, and improved the reproductive efficiency and disease and pest resistance of garlic.
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Figure CN122397619A_ABST