A method for inducing root callus of red plume sorghum
By using citric acid to synergistically regulate the root explants of *Sorghum sibirica*, the problems of browning and low induction rate in the induction of root callus in *Sorghum sibirica* were solved, achieving efficient callus induction and providing stable genetic transformation and breeding support.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- MOUTAI INST
- Filing Date
- 2026-06-15
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies lack systematic optimization schemes specifically for the induction of callus from the roots of *Sorghum sibirica*, especially lacking an efficient induction system with citric acid as an anti-browning synergist, leading to problems of high-frequency browning and low induction rate.
Citric acid was used to regulate the root explants of *Sorghum spp.* by synergistic hormone (2,4D + KT). Citric acid lowered the pH and chelated copper ions to inhibit polyphenol oxidase activity. At the same time, it provided energy and carbon skeleton as an intermediate product of the tricarboxylic acid cycle. Combined with glycine, it promoted cell division and formed vigorous callus tissue.
It significantly improved the callus induction rate, reduced the browning rate, obtained dense, light yellow high-quality callus, reduced the probability of contamination and suppuration, and provided a stable platform for genetic transformation and breeding.
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