Efficient tissue culture method of malus hallings half-lignified stem segments
By using a phased, differentiated natural product monomer compounding system and exclusive disinfection and cultivation processes, the problems of browning of explants, uneven germination, low proliferation efficiency, and weak rooting in the tissue culture of double-flowered prickly pear have been solved, realizing efficient, stable, and large-scale seedling production of double-flowered prickly pear and meeting the needs of industrial development.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUIZHOU ACAD OF SCI
- Filing Date
- 2026-06-16
- Publication Date
- 2026-07-17
AI Technical Summary
In existing technologies, seed propagation of double-flowered prickly pear suffers from severe segregation of offspring traits and a long growth cycle. Cutting propagation is limited by season and cannot meet the needs of large-scale production. Furthermore, conventional tissue culture methods result in semi-lignified stem explants being prone to browning, low axillary bud germination rate, poor proliferation efficiency of clustered buds, and difficulty in inducing rooting.
We employ a phased, differentiated natural product monomer compound system, coupled with dedicated sterilization, culture, and hardening processes. By adding specific plant hormones and natural products to the basic culture medium using MS, we optimize culture conditions, including primary culture, subculture proliferation culture, and rooting culture. Combined with gradient hardening and a dedicated substrate, we achieve efficient and stable tissue culture.
It effectively inhibits browning of explants, increases axillary bud induction rate and cluster bud proliferation coefficient, promotes root vitality, improves transplant survival rate of tissue culture seedlings, and realizes efficient, stable and large-scale seedling production of double-flowered prickly pear.
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Figure CN122397625A_ABST