A low-lactose and low-allergenic milk powder based on double-enzyme synergistic hydrolysis and a preparation method thereof

By employing a dual-enzyme synergistic hydrolysis technology that co-immobilizes lactase and protease, combined with a temperature gradient and membrane reactor, the problems of high cost, low efficiency, and poor desensitization effect in traditional dual-enzyme hydrolysis processes have been solved. This technology enables the efficient preparation of low-lactose, low-allergenic milk powder, suitable for people with lactose intolerance and mild milk protein allergy.

CN122397807APending Publication Date: 2026-07-17E-FINE HEALTH IND (YANTAI) CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-05
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies, traditional dual-enzyme hydrolysis processes are costly, have poor flavor, low efficiency, poor taste, and poor desensitization effects. They are difficult to balance the hydrolysis efficiency of lactose and milk protein, and the removal of milk protein allergens is incomplete.

Method used

A dual-enzyme synergistic hydrolysis method was adopted, in which lactase and protease were co-immobilized on a magnetic chitosan carrier, and directional anchoring and spatial positioning hydrolysis were achieved by combining emulsion interface engineering. The temperature gradient synergistic hydrolysis process was used, and the protease precursor was activated by the supernatant of thermophilic streptococcus fermentation. The bitterness was masked by membrane reactor circulation separation and polyphenol covalent modification.

Benefits of technology

It significantly reduces enzyme preparation costs, improves hydrolysis efficiency, enhances product taste and flavor, reduces milk protein allergenicity, expands the applicable population, and ensures high product stability.

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Abstract

本发明涉及乳粉制造领域,具体涉及一种基于双酶协同水解的低乳糖低致敏乳粉及其制备方法,由以下质量份的组分组成:复原乳280‑300份,复合双酶微球0.8‑1.2份等原料,本发明通过复合双酶微球,结合乳液界面工程实现双酶定向锚定与空间定位水解,采用温度梯度协同水解工艺,同时以原位生成的半乳糖稳定蛋白酶构象形成正反馈保护,再经膜反应器循环分离与多酚共价修饰掩蔽苦味并封闭过敏原表位,本发明显著降低了酶制剂使用成本,协同提高了双酶水解效率,有效改善了产品口感与风味,大幅降低了乳蛋白致敏性,且工艺集成度高、产品质量稳定,适用于乳糖不耐受及乳蛋白过敏人群食用,具有广泛的应用前景。
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Citation Information

Patent Citations

  • Streptococcus thermophilus

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