A set of salmon microsatellite loci, primer sets and applications thereof
By developing new microsatellite loci and primer sets for salmon, and utilizing next-generation sequencing and bioinformatics screening combined with fluorescence detection, the problem of insufficient accuracy in polyploid identification in existing technologies has been solved, enabling efficient and accurate polyploid breeding and population genetic analysis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- FISHERIES SCI RES INST OF JILIN PROVINCE
- Filing Date
- 2026-04-05
- Publication Date
- 2026-07-17
AI Technical Summary
Existing microsatellite markers for salmon are limited in number and lack precision in polyploid identification, making it difficult to accurately distinguish between diploid and triploid species, thus affecting the efficiency and accuracy of polyploid breeding.
We developed a new set of microsatellite loci and primer sets for salmon, screened candidate loci through next-generation sequencing and bioinformatics analysis, designed specific PCR amplification primers, and verified polymorphism by combining fluorescence detection to establish a high-precision identification method.
It significantly improves the accuracy and reliability of polyploid breeding identification, enabling rapid and accurate differentiation between diploid and triploid, thereby enhancing breeding efficiency and result stability.
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