A fluorescent rpa primer probe combination, kit and method for identifying citrus huanglongbing disease
By designing a primer-probe combination and kit for identifying citrus Huanglongbing using fluorescent RPA, the nucleic acid extraction steps were simplified, enabling rapid and convenient detection of citrus Huanglongbing. This solved the problems of complex operation and long time consumption in existing technologies, and is applicable to various sample types in the field, improving detection sensitivity and accuracy.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ZHONGKE GREEN CONTROL TECH CO LTD
- Filing Date
- 2026-04-28
- Publication Date
- 2026-07-17
AI Technical Summary
Existing citrus Huanglongbing (HLB) detection technologies are complex to operate, time-consuming, and rely on specialized equipment and personnel, making it difficult to meet the needs of real-time field testing.
Design a primer-probe combination and kit for identifying citrus Huanglongbing with fluorescent RPA, including F1 primer, R1 primer and P1 probe, combined with lysis buffer, nucleic acid dilution buffer, amplification lyophilized powder and activator, to simplify the nucleic acid extraction steps and achieve rapid and portable detection.
It enables rapid, convenient, and safe detection of Huanglongbing (HLB) in citrus, is applicable to various sample types in the field, reduces operational difficulty, avoids the risk of aerosol contamination, and improves detection sensitivity and accuracy.
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