Dpo-pcr primer pair for detecting bovine coronavirus and application thereof
By designing DPO-PCR primer pairs and utilizing flexible and specific amplification technology of polyxanthine linker regions, the problems of long detection cycles, poor specificity, and cumbersome annealing temperature optimization in bovine coronavirus detection have been solved, achieving high-sensitivity and low-cost early diagnosis and monitoring.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
- Filing Date
- 2026-05-25
- Publication Date
- 2026-07-17
AI Technical Summary
Existing bovine coronavirus detection methods are time-consuming, have poor specificity, require complex annealing temperature optimization, and are poorly applicable to grassroots settings, making it difficult to achieve early diagnosis and efficient monitoring.
A pair of DPO-PCR primers targeting the bovine coronavirus N gene was designed. Utilizing the flexible linker region of the poly-xanthine (poly-I) linker, the amplification specificity is high, the annealing temperature adaptability is wide, and the operation steps are simplified, making it suitable for primary veterinary laboratories.
It enables the detection of bovine coronavirus that is simple to operate, accurate, and highly sensitive, with a detection limit of 7.55 × 10³ copies/μL. It is applicable to a variety of samples, reduces costs, and is suitable for monitoring and diagnosis in farms and primary laboratories.
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