A p. aeruginosa magnetic molecularly imprinted colorimetric sensor based on signal amplification strategy and construction method and application thereof
By constructing a sandwich colorimetric sensor based on surface molecular imprinting technology and metal-organic framework signal amplification strategy, the problem of rapid, simple, low-cost detection of highly specific and sensitive Pseudomonas aeruginosa was solved, enabling on-site real-time detection.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- XIANGTAN UNIV
- Filing Date
- 2026-04-27
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies struggle to achieve rapid, simple, low-cost detection of Pseudomonas aeruginosa with high specificity and sensitivity without relying on expensive instruments. Traditional methods are time-consuming or have high requirements for equipment and operation, while emerging methods are costly and difficult to popularize.
By combining surface molecular imprinting technology with an acid-responsive cleavage metal-organic framework signal amplification strategy, a sandwich-structured colorimetric sensor was constructed. Bacteria were captured using MIPs and enriched through magnetic separation. Fe3+ signal molecules were released under acidic conditions using MOF signal probes to generate a visualized blood-red complex.
It achieves highly specific and sensitive detection of Pseudomonas aeruginosa, with a detection limit as low as 15 CFU/mL, a wide linear range, simple operation, and is suitable for on-site point-of-care testing. It does not require large instruments and is inexpensive.
Smart Images
Figure CN122409631A_ABST