A method for identifying response of inosine modification of brassica rapa to salt treatment by LC-MS-MS
By optimizing sample pretreatment and mass spectrometry detection using LC-MS-MS technology, the problem of detecting inosine modification response salt treatment in Chinese cabbage RNA was solved, achieving highly sensitive and specific quantitative analysis and revealing the epigenetic regulatory mechanism of Chinese cabbage.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- HEBEI AGRICULTURAL UNIV.
- Filing Date
- 2026-05-12
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies lack effective means for rapid, accurate, and sensitive quantitative detection of inosine modification response to salt treatment in Chinese cabbage RNA. In particular, the large differences in the abundance of adenosine and inosine in complex matrices lead to insufficient detection accuracy and stability, making it impossible to accurately identify the salt treatment response.
Using LC-MS-MS technology, by optimizing the sample pretreatment process and adding adenosine deaminase inhibitors and antioxidants, combined with specific mass spectrometry multiple reaction monitoring ion pairs and optimized collision energies, inosine and adenosine were quantified, and their relative abundance was calculated to correct for systematic errors, thus realizing the dynamic identification of inosine modification in Chinese cabbage.
This study improved the accuracy and stability of detection, revealed the epigenetic response patterns of Chinese cabbage in the early stages of salt treatment, and provided technical means for studying the stress resistance regulation mechanisms of crops.
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