A seaweed polysaccharide-loaded electrospun nanofiber membrane and its application as a skin wound dressing
By using electrospun nanofiber membranes loaded with seaweed polysaccharides, the problems of poor absorption and easy adhesion of film dressings have been solved. The membranes provide the antibacterial and healing-promoting functions of soft hydrogels, making them suitable for irregular wounds and reducing damage during dressing changes.
Patent Information
- Application Number
- CN202610671268.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-15
- Publication Date
- 2026-07-28
AI Technical Summary
Existing film dressings have poor absorption capacity, are prone to adhering to newly formed tissue, make it difficult to maintain a moist healing environment, and are prone to damaging the wound when changing them. Traditional gauze dressings are difficult to fix and are prone to causing secondary damage.
An electrospun nanofiber membrane loaded with seaweed polysaccharides is prepared by electrospinning technology. The inner layer is a modified polyurethane fiber membrane, and the outer layer is a three-network hydrogel of carboxymethyl agarose, sodium alginate and polyvinyl alcohol. Combined with Artemisia argyi extract and silver nitrate, a soft hydrogel is formed to provide an antibacterial and healing-promoting dressing.
It achieves efficient absorption of exudate, avoids adhesion to new tissue, reduces secondary damage during wound replacement, promotes wound healing, is suitable for irregular wounds, and has antibacterial and tissue regeneration-promoting effects.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of nanofiber membrane technology, and more specifically, to an electrospun nanofiber membrane loaded with seaweed polysaccharides and its application as a dressing for skin wounds. Background Technology
[0002] As the largest organ in the human body, the skin plays a crucial role in resisting pathogens and maintaining homeostasis. Extensive burns, severe trauma, or chronic ulcers can severely damage the skin barrier, making it susceptible to infection and even life-threatening situations. Therefore, developing novel dressings that can effectively cover wounds, prevent infection, and promote tissue regeneration has become a key focus in the field of biomaterials. Traditional gauze dressings are inexpensive, but they tend to adhere to newly formed tissue, making it difficult to maintain a moist healing environment and causing secondary damage during dressing changes.
[0003] Hydrogels, with their three-dimensional network structure similar to the extracellular matrix, excellent water content, and good biocompatibility, are widely regarded as ideal candidates for wound dressings. Sodium alginate (SA), derived from brown algae, is a biocompatible natural polysaccharide. Its molecular chain is rich in carboxyl groups, giving it electronegativity, and it can react with divalent ions (such as Ca²⁺). 2+ Cross-linking forms a stable "egg-box" structure, thereby endowing the hydrogel with a certain degree of ionic conductivity. Furthermore, SA also possesses pro-angiogenic potential, promoting wound repair by releasing pro-angiogenic factors and maintaining the survival of DPSCs. On the other hand, metal ions, as key biological signaling molecules, play various regulatory roles in the wound healing process.
[0004] In existing technologies, film dressings have poor absorption capacity, almost no ability to absorb exudate, and are unsuitable for wounds with moderate to large amounts of exudate, as exudate accumulation may induce infection. They are also difficult to fix, as the dressings themselves tend to stick together, requiring uniform tension to maintain proper position. Furthermore, the excessive adhesiveness of film dressings can damage the delicate skin around the wound if removed improperly. Summary of the Invention
[0005] This invention provides an electrospun nanofiber membrane loaded with seaweed polysaccharides and its application as a skin wound dressing. When used as a dressing, the seaweed polysaccharide-loaded electrospun nanofiber membrane, after absorbing exudate, forms a soft hydrogel on the side in contact with the wound, preventing adhesion to newly formed granulation tissue. During dressing changes, it does not cause secondary tearing damage to the wound, greatly reducing patient suffering.
[0006] In a first aspect, the present invention provides a method for preparing an electrospun nanofiber membrane loaded with seaweed polysaccharides, comprising the following steps: (1) Polycaprolactone diol is heated to 100~120℃ for 1~2h under vacuum or nitrogen protection, cooled to 60~80℃, isophorone diisocyanate is added, and the reaction is carried out for 2~3h under stirring. Then, chain extender L-lysine is added to obtain modified polyurethane solution. (2) Dissolve polyvinyl alcohol powder in deionized water, heat and stir until completely dissolved to form a transparent gel, add carboxymethyl agarose and sodium alginate in proportion, add CaSO4, MgCl2 and ZnCl2 to prepare hydrogel precursor solution; (3) The hydrogel precursor solution and the modified polyurethane solution are blended to obtain a blend solution. Silver nitrate solution is added dropwise to Artemisia argyi extract to obtain a mixed solution. The blend solution and the mixed solution are mixed to obtain a spinning solution. Electrospinning technology is used to obtain an electrospinned nanofiber membrane loaded with seaweed polysaccharide.
[0007] Preferably, in step (1), the molar ratio of polycaprolactone diol, isophorone diisocyanate and L-lysine is 2~5:1~2:0.5~1.5.
[0008] Preferably, in step (2), the heating and stirring temperature is 80~90℃ and the stirring time is 20~30min.
[0009] Preferably, in step (2), the mass ratio of carboxymethyl agarose, sodium alginate and polyvinyl alcohol is 3~5:1:2~3.
[0010] Preferably, in step (2), the mass ratio of CaSO4, MgCl2 and ZnCl2 is 1~8:2~3:1.
[0011] Preferably, in step (3), the mass ratio of the silver nitrate solution to the Artemisia argyi extract is 2~3:1.
[0012] Preferably, in step (3), the process parameters of the electrospinning technology are: voltage of 15~25 kV, receiving distance of 15~20 cm, and flow rate of 0.5~1.5 mL / h.
[0013] Secondly, the present invention provides an electrospun nanofiber membrane loaded with seaweed polysaccharides prepared by the above-mentioned method for preparing electrospun nanofiber membrane loaded with seaweed polysaccharides.
[0014] Preferably, the average pore size of the electrospun nanofiber membrane loaded with seaweed polysaccharides is 200 nm to 400 nm.
[0015] Thirdly, the present invention provides an application of an electrospun nanofiber membrane loaded with seaweed polysaccharides as a dressing for skin wounds, wherein the electrospun nanofiber membrane loaded with seaweed polysaccharides is applied to chronic, difficult-to-heal wounds.
[0016] In summary, the present invention has the following beneficial effects: 1. In this invention, seaweed polysaccharides possess a structure similar to the extracellular matrix, enabling the regulation of humidity in the wound microenvironment; L-lysine in the modified polyurethane promotes cell adhesion. Combined with the potent antibacterial capabilities of mugwort and silver ions, it effectively breaks the vicious cycle of infection and inflammation, promoting granulation tissue growth. The high specific surface area of the nanofiber membrane allows for rapid absorption of large amounts of exudate, while the modified polyurethane layer provides a physical barrier, preventing excessive moisture evaporation and bacterial invasion. Mugwort extract has certain analgesic and anti-inflammatory effects, alleviating patient suffering. The silver nitrate / mugwort complex constitutes a dual antibacterial defense; silver ions destroy bacterial DNA and cell walls, while flavonoids in mugwort disrupt bacterial membrane structures. The synergistic effect of both has a strong killing effect on both Gram-positive and Gram-negative bacteria, and is less prone to drug resistance. The breathability of the dressing's nanopores ensures oxygen exchange and promotes aerobic metabolism; the introduction of L-lysine activates fibroblasts, accelerates collagen deposition, and aids in tissue regeneration after necrotic tissue sloughs off.
[0017] 2. The inner layer of this invention, composed of carboxymethyl agarose, sodium alginate, and polyvinyl alcohol triple-network hydrogel, possesses strong hydrophilicity. It absorbs wound exudate and swells to form a gel, keeping the wound moist while preventing the dressing from adhering to the wound and causing secondary damage during dressing changes. The outer layer, a modified polyurethane fiber membrane, has hydrophobicity and a microporous structure, allowing water vapor to pass through while blocking external liquid water and bacteria from entering. Silver nitrate is reduced using Artemisia argyi extract, and the resulting silver nanoparticles are encapsulated within the fibers. The polyphenols in Artemisia argyi act not only as reducing agents but also as stabilizers, resulting in a slower and more sustained release of silver ions, avoiding the cytotoxicity of traditional silver dressings. Artemisia argyi itself has anti-inflammatory activity, which, combined with the immunomodulatory effects of sodium alginate, can reduce the level of inflammatory factors at the wound site and shorten the inflammatory period.
[0018] 3. The nanofibers in this invention mimic the structure of natural skin extracellular matrix. The L-lysine in the modified polyurethane provides cell recognition sites (-NH2 groups), significantly improving fibroblast adhesion and proliferation rates, and accelerating wound contraction and healing. This solves the problems of traditional gauze easily adhering to wounds and having limited functionality, while also overcoming the poor mechanical strength of simple hydrogels. The modified polyurethane, composed of polycaprolactone soft segments and L-lysine hard segments, endows the fiber membrane with excellent elasticity and tensile properties. This allows the dressing to closely adhere to wounds in irregular or frequently moving areas such as joints and fingertips, preventing breakage or detachment during limb movement, thus aiding in the treatment of complex and difficult-to-heal wounds such as diabetic ulcers, burns, trauma, and drug-resistant bacterial infections.
[0019] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit the scope of protection of the present invention. Detailed Implementation
[0020] The present invention will be further described in detail below with reference to the embodiments. It should be noted that: unless otherwise specified, the conditions in the following embodiments are carried out according to conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, the raw materials used in the following embodiments can be obtained from commercially available sources.
[0021] Example Example 1 A method for preparing an electrospun nanofiber membrane loaded with seaweed polysaccharides includes the following steps: (1) Polycaprolactone diol is heated to 100°C for 1 hour under vacuum or nitrogen protection, cooled to 60°C, and isophorone diisocyanate is added. The mixture is reacted for 2 hours under stirring, and chain extender L-lysine is added to obtain a modified polyurethane solution. The molar ratio of polycaprolactone diol, isophorone diisocyanate and L-lysine is 2:1:0.5.
[0022] (2) Dissolve polyvinyl alcohol powder in deionized water, heat and stir until completely dissolved to form a transparent gel solution, add carboxymethyl agarose and sodium alginate in proportion, add CaSO4, MgCl2 and ZnCl2 to prepare hydrogel precursor solution; the heating and stirring temperature is 80℃ and the stirring time is 20min; the mass ratio of carboxymethyl agarose, sodium alginate and polyvinyl alcohol is 3:1:2; the mass ratio of CaSO4, MgCl2 and ZnCl2 is 5:2:1.
[0023] (3) The hydrogel precursor solution and the modified polyurethane solution were blended to obtain a blend solution. Silver nitrate solution was added dropwise to Artemisia argyi extract to obtain a mixed solution. The blend solution and the mixed solution were mixed to obtain a spinning solution. Electrospinning technology was used to obtain an electrospun nanofiber membrane loaded with seaweed polysaccharide. The mass ratio of silver nitrate solution to Artemisia argyi extract was 2:1. The process parameters of electrospinning technology were: voltage of 15kV, receiving distance of 15cm, and flow rate of 1.5 mL / h.
[0024] The average pore size of the electrospun nanofiber membrane loaded with seaweed polysaccharides was 200 nm.
[0025] Example 2 A method for preparing an electrospun nanofiber membrane loaded with seaweed polysaccharides includes the following steps: (1) Polycaprolactone diol is heated to 110°C for 1 hour under vacuum or nitrogen protection, cooled to 60°C, isophorone diisocyanate is added, and the mixture is reacted for 2 hours under stirring. Then, chain extender L-lysine is added to obtain a modified polyurethane solution. The molar ratio of polycaprolactone diol, isophorone diisocyanate and L-lysine is 2:1:1.
[0026] (2) Dissolve polyvinyl alcohol powder in deionized water, heat and stir until completely dissolved to form a transparent gel solution, add carboxymethyl agarose and sodium alginate in proportion, add CaSO4, MgCl2 and ZnCl2 to prepare hydrogel precursor solution; the heating and stirring temperature is 85℃ and the stirring time is 20min; the mass ratio of carboxymethyl agarose, sodium alginate and polyvinyl alcohol is 3:1:2; the mass ratio of CaSO4, MgCl2 and ZnCl2 is 3:2:1.
[0027] (3) The hydrogel precursor solution and the modified polyurethane solution were blended to obtain a blend solution. Silver nitrate solution was added dropwise to Artemisia argyi extract to obtain a mixed solution. The blend solution and the mixed solution were mixed to obtain a spinning solution. Electrospinning technology was used to obtain an electrospun nanofiber membrane loaded with seaweed polysaccharide. The mass ratio of silver nitrate solution to Artemisia argyi extract was 2:1. The process parameters of electrospinning technology were: voltage of 15kV, receiving distance of 15 cm, and flow rate of 0.5 mL / h.
[0028] The average pore size of the electrospun nanofiber membrane loaded with seaweed polysaccharides was 230 nm.
[0029] Example 3 A method for preparing an electrospun nanofiber membrane loaded with seaweed polysaccharides includes the following steps: (1) Polycaprolactone diol is heated to 100°C for 2 hours under vacuum or nitrogen protection, cooled to 80°C, isophorone diisocyanate is added, and the mixture is reacted for 3 hours under stirring. Then, chain extender L-lysine is added to obtain a modified polyurethane solution. The molar ratio of polycaprolactone diol, isophorone diisocyanate and L-lysine is 3:2:0.5.
[0030] (2) Dissolve polyvinyl alcohol powder in deionized water, heat and stir until completely dissolved to form a transparent gel solution, add carboxymethyl agarose and sodium alginate in proportion, add CaSO4, MgCl2 and ZnCl2 to prepare hydrogel precursor solution; the heating and stirring temperature is 85℃ and the stirring time is 20min; the mass ratio of carboxymethyl agarose, sodium alginate and polyvinyl alcohol is 5:1:2; the mass ratio of CaSO4, MgCl2 and ZnCl2 is 7:2:1.
[0031] (3) The hydrogel precursor solution and the modified polyurethane solution were blended to obtain a blend solution. Silver nitrate solution was added dropwise to Artemisia argyi extract to obtain a mixed solution. The blend solution and the mixed solution were mixed to obtain a spinning solution. Electrospinning was used to obtain an electrospun nanofiber membrane loaded with seaweed polysaccharide. The mass ratio of silver nitrate solution to Artemisia argyi extract was 3:1. The process parameters of electrospinning were: voltage 20 kV, receiving distance 20 cm, and flow rate 1.2 mL / h.
[0032] The average pore size of the electrospun nanofiber membrane loaded with seaweed polysaccharides was 250 nm.
[0033] Example 4 A method for preparing an electrospun nanofiber membrane loaded with seaweed polysaccharides includes the following steps: (1) Polycaprolactone diol is heated to 110°C for 1 hour under vacuum or nitrogen protection, cooled to 70°C, isophorone diisocyanate is added, and the mixture is reacted for 2 hours under stirring. Then, chain extender L-lysine is added to obtain a modified polyurethane solution. The molar ratio of polycaprolactone diol, isophorone diisocyanate and L-lysine is 3:1:0.9.
[0034] (2) Dissolve polyvinyl alcohol powder in deionized water, heat and stir until completely dissolved to form a transparent gel solution, add carboxymethyl agarose and sodium alginate in proportion, add CaSO4, MgCl2 and ZnCl2 to prepare hydrogel precursor solution; the heating and stirring temperature is 80℃ and the stirring time is 30min; the mass ratio of carboxymethyl agarose, sodium alginate and polyvinyl alcohol is 3:1:3; the mass ratio of CaSO4, MgCl2 and ZnCl2 is 5:3:1.
[0035] (3) The hydrogel precursor solution and the modified polyurethane solution were blended to obtain a blend solution. Silver nitrate solution was added dropwise to Artemisia argyi extract to obtain a mixed solution. The blend solution and the mixed solution were mixed to obtain a spinning solution. Electrospinning was used to obtain an electrospun nanofiber membrane loaded with seaweed polysaccharide. The mass ratio of silver nitrate solution to Artemisia argyi extract was 2:1. The process parameters of electrospinning were: voltage 25 kV, receiving distance 20 cm, and flow rate 1.5 mL / h.
[0036] The average pore size of the electrospun nanofiber membrane loaded with seaweed polysaccharides was 300 nm.
[0037] Example 5 A method for preparing an electrospun nanofiber membrane loaded with seaweed polysaccharides includes the following steps: (1) Polycaprolactone diol is heated to 120°C for 2 hours under vacuum or nitrogen protection to remove water, then cooled to 80°C, isophorone diisocyanate is added, and the mixture is reacted for 3 hours under stirring. Then, chain extender L-lysine is added to obtain a modified polyurethane solution. The molar ratio of polycaprolactone diol, isophorone diisocyanate and L-lysine is 5:2:1.5.
[0038] (2) Dissolve polyvinyl alcohol powder in deionized water, heat and stir until completely dissolved to form a transparent gel solution, add carboxymethyl agarose and sodium alginate in proportion, add CaSO4, MgCl2 and ZnCl2 to prepare hydrogel precursor solution; the heating and stirring temperature is 90℃ and the stirring time is 30min; the mass ratio of carboxymethyl agarose, sodium alginate and polyvinyl alcohol is 5:1:3; the mass ratio of CaSO4, MgCl2 and ZnCl2 is 8:3:1.
[0039] (3) The hydrogel precursor solution and the modified polyurethane solution were blended to obtain a blend solution. Silver nitrate solution was added dropwise to Artemisia argyi extract to obtain a mixed solution. The blend solution and the mixed solution were mixed to obtain a spinning solution. Electrospinning was used to obtain an electrospun nanofiber membrane loaded with seaweed polysaccharide. The mass ratio of silver nitrate solution to Artemisia argyi extract was 3:1. The process parameters of electrospinning were: voltage 25 kV, receiving distance 20 cm, and flow rate 1.5 mL / h.
[0040] The average pore size of the electrospun nanofiber membrane loaded with seaweed polysaccharides was 400 nm.
[0041] Comparative Example 1 The difference from Example 1 is that no modified polyurethane solution was added.
[0042] Comparative Example 2 The difference from Example 1 is that no silver nitrate solution was added.
[0043] Comparative Example 3 The difference from Example 1 is that no Artemisia argyi extract was added.
[0044] Table 1 Performance Test Results:
[0045] As shown in Table 1, the electrospun nanofiber membrane loaded with seaweed polysaccharides prepared in Example 1 exhibits excellent performance. When used as a dressing, after absorbing exudate, it forms a soft hydrogel on the side in contact with the wound, preventing adhesion to newly formed granulation tissue. When changing the dressing, it does not cause secondary tearing damage to the wound, greatly reducing patient suffering.
[0046] The above description is merely an exemplary embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.
Claims
1. A method for preparing an electrospun nanofiber membrane loaded with seaweed polysaccharides, characterized in that, Includes the following steps: (1) Polycaprolactone diol is heated to 100~120℃ for 1~2h under vacuum or nitrogen protection, cooled to 60~80℃, isophorone diisocyanate is added, and the reaction is carried out for 2~3h under stirring. Then, chain extender L-lysine is added to obtain modified polyurethane solution. (2) Dissolve polyvinyl alcohol powder in deionized water, heat and stir until completely dissolved to form a transparent gel, add carboxymethyl agarose and sodium alginate in proportion, add CaSO4, MgCl2 and ZnCl2 to prepare hydrogel precursor solution; (3) The hydrogel precursor solution and the modified polyurethane solution are blended to obtain a blend solution. Silver nitrate solution is added dropwise to Artemisia argyi extract to obtain a mixed solution. The blend solution and the mixed solution are mixed to obtain a spinning solution. Electrospinning technology is used to obtain an electrospinned nanofiber membrane loaded with seaweed polysaccharide.
2. The method for preparing electrospun nanofiber membranes loaded with seaweed polysaccharides according to claim 1, characterized in that, In step (1), the molar ratio of polycaprolactone diol, isophorone diisocyanate and L-lysine is 2~5:1~2:0.5~1.
5.
3. The method for preparing electrospun nanofiber membranes loaded with seaweed polysaccharides according to claim 1, characterized in that, In step (2), the heating and stirring temperature is 80~90℃, and the stirring time is 20~30min. 。 4. The method for preparing electrospun nanofiber membranes loaded with seaweed polysaccharides according to claim 1, characterized in that, In step (2), the mass ratio of carboxymethyl agarose, sodium alginate and polyvinyl alcohol is 3~5:1:2~3.
5. The method for preparing electrospun nanofiber membranes loaded with seaweed polysaccharides according to claim 1, characterized in that, In step (2), the mass ratio of CaSO4, MgCl2 and ZnCl2 is 1~8:2~3:
1.
6. The method for preparing electrospun nanofiber membranes loaded with seaweed polysaccharides according to claim 1, characterized in that, In step (3), the mass ratio of the silver nitrate solution to the Artemisia argyi extract is 2~3:
1.
7. The method for preparing electrospun nanofiber membranes loaded with seaweed polysaccharides according to claim 1, characterized in that, In step (3), the process parameters of the electrospinning technology are: voltage of 15~25 kV, receiving distance of 15~20 cm, and flow rate of 0.5~1.5 mL / h.
8. An electrospun nanofiber membrane loaded with seaweed polysaccharides prepared by the method described in any one of claims 1 to 7.
9. The electrospun nanofiber membrane loaded with seaweed polysaccharides according to claim 8, characterized in that, The average pore size of the electrospun nanofiber membrane loaded with seaweed polysaccharides is 200 nm to 400 nm.
10. The application of the electrospun nanofiber membrane loaded with seaweed polysaccharides as described in claims 8-9 as a skin wound dressing, characterized in that, The electrospun nanofiber membrane loaded with seaweed polysaccharides is applied to chronic, difficult-to-heal wounds.