A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract and its application.

Abstract

This invention provides a moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract and its application, relating to the field of cosmetic technology. The composition comprises the following ingredients: Cornus officinalis extract, beetroot extract, *Prickly pear* fruit extract, *Chrysanthemum indicum* extract, and carboxymethyl deacetylated chitosan. This invention overcomes the limitations of traditional repair products that rely on only a single ingredient. Through a synergistic five-component system of Cornus officinalis extract, carboxymethyl deacetylated chitosan, beetroot extract, *Prickly pear* fruit extract, and *Chrysanthemum indicum* extract, it simultaneously achieves moisturizing, water-locking, barrier repair, and soothing and anti-allergic effects, and is suitable for various sensitive skin types, being gentle and safe.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to a moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract and its application. Background Technology

[0002] As the outermost defensive barrier of the human body, the skin is composed of the stratum corneum, sebum film, and skin microecology. It plays a vital role in locking in moisture, resisting external stimuli, and preventing the penetration of allergens. Its health directly affects the skin's appearance and physiological function. With the accelerated pace of life, increased environmental pollutants, enhanced ultraviolet radiation, and unhealthy skincare habits such as over-cleansing and misuse of functional skincare products, skin barrier damage is becoming increasingly common, and the number of people with sensitive skin continues to expand. Damaged skin often manifests as dryness, tightness, redness, and stinging, with a significantly decreased tolerance to external stimuli. In severe cases, itching, flaking, and inflammation may occur, not only affecting appearance but also reducing the skin's self-repair ability, creating a vicious cycle of "sensitivity - damaged barrier - increased sensitivity."

[0003] To address the aforementioned skin problems, various moisturizing, repairing, and anti-allergic skincare products have emerged on the market. For example, Chinese patent application CN114344191A discloses a moisturizing facial mask liquid and a facial mask. The raw materials of the facial mask liquid include a moisturizing agent with a mass fraction of 8.5-19.5%, which is composed of 1,3-butanediol, β-glucan, and sialic acid in a mass ratio of (2-8):(6-10):(0.5-1.5). By using 1,3-butanediol, β-glucan, and sialic acid in a specific combination as moisturizing agents, a long-lasting moisturizing and water-locking effect comparable to existing technologies is achieved while significantly reducing the number of moisturizing ingredients. Chinese patent application CN111728907A discloses a soothing and anti-allergic composition, its preparation method, and its application in cosmetics. The soothing and anti-allergic composition comprises the following components in parts by weight: 5-50 parts of peony root, 3-35 parts of dandelion, 5-30 parts of mimosa bark, and 15-40 parts of purslane. This invention's soothing and anti-allergic composition does not contain any other chemical moisturizers, preservatives, or antibacterial agents. It is a composition of pure natural plant ingredients, and the resulting product is gentle and non-irritating, suitable for long-term use.

[0004] However, existing related products and their compositions still have many shortcomings: on the one hand, most products only focus on a single function, or only focus on moisturizing without effective barrier repair and anti-allergy effects, or only emphasize anti-allergy while the moisturizing effect is poor, and cannot achieve simultaneous improvement in moisturizing, repair and anti-allergy, making it difficult to solve the problem of damaged skin barrier and sensitivity from the root; on the other hand, some products add high concentrations of chemically synthesized ingredients, preservatives or fragrances in pursuit of rapid effects, which not only easily aggravate skin irritation, but may also disrupt the skin's microecological balance, leading to repeated sensitivity problems, and cannot meet the long-term care needs of fragile skin types such as sensitive skin and skin with damaged barrier.

[0005] In addition, while there are some plant-based repair compositions in existing technologies, many suffer from problems such as unreasonable ingredient combinations and unclear synergistic effects. Either the content of active ingredients is insufficient, resulting in limited efficacy, or there are antagonistic effects between ingredients, affecting product stability and effectiveness. At the same time, the preparation process of some plant extracts is not optimized enough, leading to the loss of their core active ingredients, which further reduces the repair and anti-allergy efficacy of the composition.

[0006] Therefore, developing a composition with a reasonable combination of ingredients, significant synergistic effects, high safety, and the ability to simultaneously moisturize and lock in moisture, repair the skin barrier, soothe and reduce sensitivity, and be suitable for various fragile skin types and applied to cosmetics has become a pressing technical problem to be solved in the cosmetics industry. Summary of the Invention

[0007] To address the aforementioned problems, this invention provides a moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract and its application. Through the specific compounding of components, it can simultaneously achieve moisturizing and water-locking, barrier repair, and soothing and anti-allergic effects, and is suitable for various fragile skin types, making it gentle and safe.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] On the one hand, the present invention provides a moisturizing, repairing and anti-allergic composition containing Cornus officinalis extract, comprising the following raw materials: Cornus officinalis extract, beetroot extract, sea buckthorn fruit extract, Chrysanthemum indicum extract and carboxymethyl deacetylated chitosan.

[0010] Preferably, the mass ratio of the Cornus officinalis extract, Chrysanthemum indicum extract, and carboxymethyl deacetylated chitosan is 4-6:2-4:0.1-1.5.

[0011] Any point value or any sub-range value within the range of 4:6:2-4:0.1-1.5 is applicable to this invention, including but not limited to 4:2:0.1, 4:2:0.2, 4:2:0.3, 4:2:0.4, 4:2:0.5, 4:2:0.6, 4:2:0.7, 4:2:0.8, 4:2:0.9, 4:2:1, 4:2:1.1, 4:2:1.2, 4:2:1.3, 4:2:1.4, 4:2:1.5, 5:2:0.1, 5:2:0.2, 5:2:0.3, 5:2:0.4, 5:2:0.5, 5:2:0.6, 5:2:0.7, 5:2:0.8, 5:2:0.9, 5:2:1, 5:2:1.1, 5:2:1.2, 5:2:1.3, 5:2:1.4, 5:2:1.5, 6:2:0.1, 6:2:0.2, 6:2:0.3, 6:2:0.4, 6:2:0.5, 6:2:0.6, 6:2:0.7, 6:2:0.8, 6:2:0.9, 6:2:1, 6:2:1.1, 6:2:1. 2, 6:2:1.3, 6:2:1.4, 6:2:1.5, 4:3:0.1, 4:3:0.2, 4:3:0.3, 4:3:0.4, 4:3:0.5, 4:3:0.6, 4:3:0.7, 4:3:0.8, 4:3:0.9, 4:3:1, 4:3:1.1, 4:3:1.2, 4:3:1 3, 4:3:1.4, 4:3:1.5, 5:3:0.1, 5:3:0.2, 5:3:0.3, 5:3:0.4, 5:3:0.5, 5:3:0.6, 5:3:0.7, 5:3:0.8, 5:3:0.9, 5:3:1, 5:3:1.1, 5:3:1.2, 5:3:1.3, 5:3 :1.4, 5:3:1.5, 6:3:0.1, 6:3:0.2, 6:3:0.3, 6:3:0.4, 6:3:0.5, 6:3:0.6, 6:3:0.7, 6:3:0.8, 6:3:0.9, 6:3:1, 6:3:1.1, 6:3:1.2, 6:3:1.3, 6:3:1.4, 6 :3:1.5, 4:4:0.1, 4:4:0.2, 4:4:0.3, 4:4:0.4, 4:4:0.5, 4:4:0.6, 4:4:0.7, 4:4:0.8, 4:4:0.9, 4:4:1, 4:4:1.1, 4:4:1.2, 4:4:1.3, 4:4:1.4, 4:4:1.5 5:4:0.1, 5:4:0.2, 5:4:0.3, 5:4:0.4, 5:4:0.5, 5:4:0.6, 5:4:0.7, 5:4:0.8, 5:4:0.9, 5:4:1, 5:4:1.1, 5:4:1.2, 5:4:1.3, 5:4:1.4, 5:4:1.5, 6:4:0.1. 6:4:0.2, 6:4:0.3, 6:4:0.4, 6:4:0.5, 6:4:0.6, 6:4:0.7, 6:4:0.8, 6:4:0.9, 6:4:1, 6:4:1.1, 6:4:1.2, 6:4:1.3, 6:4:1.4, 6:4:1.5.

[0012] More preferably, the mass ratio of the Cornus officinalis extract, Chrysanthemum indicum extract and carboxymethyl deacetylated chitosan is 4-6:2-4:0.5-1.5.

[0013] More preferably, the mass ratio of the Cornus officinalis extract, Chrysanthemum indicum extract and carboxymethyl deacetylated chitosan is 5:3:1.

[0014] Preferably, the preparation method of the *Cyprinus sarmentosus* fruit extract includes the following steps: after crushing the *Cyprinus sarmentosus* seeds, add 50-60 wt% ethanol solution, extract once at 50-60℃ for 10-30 min under ultrasonic assistance, then add ethanol to the solution to an ethanol concentration of 70-80 wt%, extract a second time at 35-45℃ for 10-30 min under ultrasonic assistance, combine the extracts, purify with macroporous resin, and then dry to obtain the extract.

[0015] More preferably, the preparation method of the *Cyprinus pungens* fruit extract includes the following steps: *Cyprinus pungens* seeds are washed, dried, pulverized, and passed through an 80-100 mesh sieve. A 50-60 wt% ethanol solution is added at a material-to-liquid ratio of 1g:10-20mL. Extraction is performed once at 50-60℃ for 10-30min under ultrasonic assistance. Then, ethanol is added until the ethanol concentration in the solution is 70-80 wt%. Extraction is performed a second time at 35-45℃ for 10-30min under ultrasonic assistance. The extracts are combined, and D101 macroporous resin is used for elution, followed by pure water and 70-80 wt% ethanol solution. 4 BV of the 70-80 wt% ethanol eluent is collected, dried, and the extract is obtained.

[0016] Preferably, the preparation method of the Shennong Xiangju extract includes the following steps: fresh Shennong Xiangju flowers are crushed and mixed with water, hydrolyzed by cellulase and pectinase, extracted with 55-65wt% ethanol, separated by nanofiltration membrane to remove macromolecular volatile oils, and then dried to obtain the extract.

[0017] More preferably, the preparation method of the Shennong Xiangju extract includes the following steps: washing, drying, pulverizing, and passing the fresh Shennong Xiangju flowers through an 80-100 mesh sieve; mixing with water at a material-to-liquid ratio of 1g:10-20mL; adding cellulase and pectinase at a mass ratio of 2:1 for enzymatic hydrolysis, wherein the mass of the cellulase and pectinase is 0.5%-2% of the mass of the fresh Shennong Xiangju flowers; the enzymatic hydrolysis temperature is 45-50℃ and the time is 1-2h; extracting with 55-65wt% ethanol at 40-50℃ for 10-30min; separating and removing macromolecular volatile oils through a 300-400Da nanofiltration membrane; and drying to obtain the extract.

[0018] Preferably, the ingredients, by weight, include the following: 8-12 parts Cornus officinalis extract, 1-10 parts Beetroot extract, 1-10 parts Prickly pear fruit extract, 4-8 parts Chrysanthemum indicum extract and 1-3 parts carboxymethyl deacetylated chitosan.

[0019] Any point value or any sub-range value within the range of 8-12 parts of Cornus officinalis extract can be applied to this invention, including but not limited to 8 parts, 9 parts, 10 parts, 11 parts, and 12 parts.

[0020] Any point value or any sub-range value within the range of 1-10 parts of beetroot extract can be applied to this invention, including but not limited to 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, and 10 parts.

[0021] Any point value or any sub-range value within the range of 1-10 for the extract of *Cynanchum paniculatum* fruit can be applied to this invention, including but not limited to 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, and 10 parts.

[0022] Any point value or any sub-range value within the range of 4-8 parts of Shennong Xiangju extract can be applied to this invention, including but not limited to 4 parts, 5 parts, 6 parts, 7 parts, and 8 parts.

[0023] Any point value or any sub-range value within the range of 1-3 parts of carboxymethyl deacetylated chitosan can be applied to this invention, including but not limited to 1 part, 1.5 parts, 2 parts, 2.5 parts, and 3 parts.

[0024] More preferably, the ingredients, by weight, include the following: 10 parts Cornus officinalis extract, 6 parts Beetroot extract, 4 parts Prickly pear extract, 6 parts Chrysanthemum indicum extract and 2 parts carboxymethyl deacetylated chitosan.

[0025] Secondly, the present invention provides a method for preparing the above composition, comprising the following steps: mixing raw materials.

[0026] Thirdly, the present invention provides the application of the above composition in the preparation of cosmetics with moisturizing, repairing and anti-allergic effects.

[0027] Preferably, the cosmetics include facial cleanser, toner, essence water, serum, face cream, face mask, eye cream, body lotion, body oil, hand cream, and foot cream.

[0028] More preferably, the cosmetic is a face mask.

[0029] Compared with the prior art, the present invention has the following beneficial effects: 1. This invention significantly enhances repair efficacy through multi-mechanism synergy, overcoming the limitations of traditional repair products that rely solely on single ingredients (such as moisturizing or soothing only). It constructs a triple-barrier repair mechanism of "physical-lipid-cellular" through a five-element synergistic system of Cornus officinalis extract, carboxymethyl deacetylated chitosan, beetroot extract, sea buckthorn fruit extract, and chrysanthemum extract: Physical barrier: Carboxymethyl deacetylated chitosan forms a breathable protective film on the skin surface, instantly locking in moisture and blocking external stimuli; Lipid barrier: Sea buckthorn fruit extract replenishes intercellular lipids, repairing damaged "brick-and-mortar structure" and reducing transepidermal water loss (TEWL); Cellular barrier: Chrysanthemum extract and beetroot extract synergistically inhibit the release of inflammatory factors, alleviating neurogenic sensitivity. Experimental data show that the moisturizing, water-locking, repairing, and anti-allergic effects of this composition are significantly superior to single-ingredient or conventional compound combinations.

[0030] 2. All active ingredients in this invention are derived from natural plants or bio-fermentation sources, and contain no synthetic pigments, alcohol, or traditional irritating preservatives. Specifically designed for sensitive skin prone to irritation, it utilizes the natural anti-inflammatory activity of chrysanthemum extract to replace chemical soothing agents; and uses beetroot extract to regulate cell osmotic pressure, reducing environmental stressor damage to the skin. Patch testing shows that the product of this invention does not cause stinging, redness, or other adverse reactions during use, demonstrating excellent skin compatibility and safety.

[0031] 3. The technical solution of this invention has a simple preparation process, controllable cost, is easy to industrialize and has a wide range of applications: the preparation process of the core plant extract is optimized and reasonable, which can effectively retain its core active ingredients, and the process steps are simple and easy to operate. It does not require complex and expensive equipment, and the production cost is controllable, making it suitable for large-scale industrial production; the composition has good compatibility and can be flexibly applied to mask products, and can also be extended to various cosmetic formulations such as serums, creams, and lotions, with high commercial conversion value. Detailed Implementation

[0032] To make the technical means, creative features, achieved objectives, and effects of this invention readily understandable, the invention is further illustrated below with specific embodiments. However, these embodiments are merely preferred embodiments and not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments described herein without creative effort are all within the scope of protection of this invention. It is worth noting that the raw materials used in this invention are all common commercially available products, and their sources are not specifically limited. The technical and scientific terms used in the embodiments have the meanings commonly understood by those skilled in the art to which this invention pertains.

[0033] Cornus officinalis extract: purchased from Lanzhou Waterles Biotechnology Co., Ltd., specification 10:1.

[0034] Beetroot extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd., specification 10:1.

[0035] Carboxymethyl deacetylated chitosan: purchased from Hubei Langbowan Biomedical Co., Ltd., CAS: 83512-85-0.

[0036] The preparation method of the extract of *Cyprinus pungens* includes the following steps: *Cyprinus pungens* seeds are washed, dried, pulverized, and passed through a 100-mesh sieve. A 55wt% ethanol solution is added at a material-to-liquid ratio of 1g:15mL. Extraction is performed once at 55℃ for 20min under ultrasonic assistance. Then, ethanol is added until the ethanol concentration in the solution is 75wt%, and extraction is performed a second time at 40℃ for 30min under ultrasonic assistance. The extracts are combined, and D101 macroporous resin is used for elution with pure water and 75wt% ethanol solution sequentially. 4BV of the 75wt% ethanol eluent is collected, dried, and the *Cyprinus pungens* extract is obtained, which is used in the embodiments of this invention.

[0037] The preparation method of Shennong Xiangju extract includes the following steps: fresh Shennong Xiangju flowers are washed, dried, pulverized and passed through a 100-mesh sieve. They are mixed with water at a material-to-liquid ratio of 1g:16mL. Cellulase and pectinase at a mass ratio of 2:1 are added for enzymatic hydrolysis. The mass of cellulase and pectinase is 1.5% of the mass of fresh Shennong Xiangju flowers. The hydrolysis temperature is 45℃ and the time is 1.5h. Extraction is performed with 60wt% ethanol at 45℃ for 25min. After separation and removal of macromolecular volatile oils by a 350 Da nanofiltration membrane, the extract is dried to obtain Shennong Xiangju extract, which is used in the embodiments of this invention.

[0038] Example 1 A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract, comprising, by weight, the following ingredients: 10 parts Cornus officinalis extract, 6 parts Beetroot extract, 4 parts Prickly pear extract, 6 parts Chrysanthemum indicum extract, and 2 parts Carboxymethyl deacetylated chitosan.

[0039] The above raw materials are mixed to obtain a composition.

[0040] Example 2 A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract, comprising, by weight, the following ingredients: 8 parts Cornus officinalis extract, 10 parts Beetroot extract, 1 part Prickly pear fruit extract, 8 parts Chrysanthemum indicum extract, and 1 part carboxymethyl deacetylated chitosan.

[0041] The above raw materials are mixed to obtain a composition.

[0042] Example 3 A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract, comprising, by weight, the following ingredients: 12 parts Cornus officinalis extract, 10 parts Beetroot extract, 1 part Prickly pear fruit extract, 4 parts Chrysanthemum indicum extract, and 3 parts carboxymethyl deacetylated chitosan.

[0043] The above raw materials are mixed to obtain a composition.

[0044] Comparative Example 1 A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract, compared to Example 1, differs only in the amount of raw materials used, specifically as follows: The product is composed of the following ingredients by weight: 5 parts Cornus officinalis extract, 12 parts Beetroot extract, 0.5 parts Prickly pear extract, 10 parts Chrysanthemum indicum extract and 0.5 parts carboxymethyl deacetylated chitosan.

[0045] Comparative Example 2 A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract, which is identical to Example 1 except that the chamomile extract is replaced with chamomile extract.

[0046] Comparative Example 3 A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract, which is identical to Example 1 except that carboxymethyl deacetylated chitosan is replaced with sodium hyaluronate.

[0047] Test Example 1 Safety: Patch Test Human patch test Experimental Procedure: A total of 30 subjects were randomly selected based on their gender and age composition (meeting the inclusion and exclusion criteria of the "Diagnostic Criteria and Treatment Principles for Cosmetic Contact Dermatitis"). Qualified patch materials were used. The compositions prepared in Examples 1-3 and Comparative Examples 1-3 were mixed with white petrolatum at a 1:1 mass ratio and ground into a uniform paste / ointment. This paste / ointment was then added to the patch applicator as the sample group, with the control well serving as the blank control (white petrolatum). Both the sample group and the blank control were applied to the flexor side of the forearm of each subject (each subject had 7 application points, corresponding to the samples prepared in Examples 1-3 and Comparative Examples 1-3, and the blank control, respectively). The patches were gently pressed with the palm of the hand to ensure even application to the skin for 24 hours. After removing the patch applicator, the skin reaction was observed after a 30-minute interval until the pressure marks disappeared. Skin reactions were observed again at 24 and 48 hours after the patch test.

[0048] Judgment criteria: According to the "Diagnostic Criteria and Treatment Principles for Contact Dermatitis Caused by Cosmetics", the following classifications are used: no reaction at the test site (Grade 0), light red spots on the skin (Grade 1), erythema, infiltration or papules on the skin (Grade 2), edematous erythema and papules on the skin (Grade 3), and significant redness and swelling on the skin with papules or bullae (Grade 4).

[0049] If more than two out of 30 cases experience grade 2 adverse skin reactions, or if any one case experiences a grade 3 or higher adverse skin reaction, the test substance is deemed to have an adverse effect on the human body.

[0050] The specific results are shown in Table 1 below: Table 1

[0051] The results show that the compositions prepared in Examples 1-3 and Comparative Examples 1-3 of the present invention have no adverse reactions on the human body.

[0052] Test Example 2 In vitro gravimetric method for moisturizing performance testing Weigh 0.1g of the compositions prepared in Examples 1-3 and Comparative Examples 1-3 respectively, dissolve them in 5mL of glycerin, and evenly coat them onto 5cm×5cm glass plates with microporous ventilation tape. Place the glass plates in a desiccator with constant temperature and humidity, weigh the glass plates after 2h and 4h respectively, and calculate their moisture retention rate.

[0053] The formula for calculating the moisturizing rate is: Moisturizing rate inhibition rate / % = (M2-M0) / (M1-M0)×100%.

[0054] Where M0 is the mass of the glass plate in g, M1 is the mass of the glass plate after sample addition in g, and M2 is the mass of the glass plate in g after being placed in the desiccator for a certain number of hours.

[0055] The specific test results are shown in Table 2: Table 2

[0056] The results show that the compositions prepared in Examples 1-3 of this invention have better moisturizing properties compared with Comparative Examples 1-3.

[0057] Test Example 3 External soothing and anti-allergic effects 1. Experimental Principle Hyaluronic acid is a component of the tissue matrix that restricts the diffusion of water and other extracellular substances. When hydrolyzed by hyaluronidase, it makes the intercellular spaces non-adhesive, causing cell degranulation and the release of newly synthesized mediators, exerting biological effects and leading to immediate-type hypersensitivity reactions. Hyaluronidase is a participant in allergic reactions and is strongly correlated with allergies; therefore, the inhibitory effect of a test substance on hyaluronidase is often used to reflect its effect on alleviating and improving type I hypersensitivity reactions.

[0058] 2. Reagent preparation (1) Hyaluronidase: Prepare hyaluronidase at a concentration of 100 U / mL using acetate buffer solution as solvent. It should be prepared and used immediately and should not be left overnight.

[0059] (2) Potassium hyaluronic acid: Prepare potassium hyaluronic acid with a concentration of 0.5 mg / mL using acetic acid buffer solution as solvent.

[0060] (3) Buffer solution: 4.8 mL of solution A (0.2 mol / L acetic acid, i.e., 11.55 mL of glacial acetic acid dissolved in 1000 mL of distilled water) and 45.2 mL of solution B (0.2 mol / L sodium acetate, i.e., 16.4 g of CH3COONa or 27.2 g of CH3COONa·3H2O dissolved in 1000 mL of distilled water) are mixed and diluted to 100 mL to prepare an acetate buffer solution with pH = 5.6.

[0061] (4) Acetic acid acetone solution: Mix 50 mL of 1.0 mol / L sodium carbonate solution and 3.5 mL of acetylacetone solution evenly (prepare fresh before use).

[0062] (5) Preparation of p-dimethylaminobenzaldehyde (P-DAB) colorimetric reagent: Mix 0.8g of p-dimethylaminobenzaldehyde with 15mL of HCl and 15mL of anhydrous ethanol evenly before use.

[0063] (6) CaCl2 solution: 0.25 mol / L.

[0064] (7) NaOH solution: 5 mol / L.

[0065] 3. Experimental Procedure: Take 0.1 mL of CaCl2 solution (0.25 mol / L) and 0.5 mL of hyaluronidase solution (100 U / mL) and react in a water bath at 37℃ for 20 min; add 0.5 g of the sample solution (the compositions prepared in Examples 1-3 and Comparative Examples 1-3) to 50 mL of pH 5.6 solution. Acetic acid buffer solution (stirred evenly to obtain 0.5 mL), incubate for 20 min; then add 0.5 mL sodium hyaluronate solution (0.5 g / L), react for 30 min, then remove and cool for 5 min; add 0.1 mL sodium hydroxide solution (0.4 mol / L) and 0.5 mL acetylacetone solution (3.5 mL acetylacetone dissolved in 50 mL 1.0 mol / L sodium carbonate solution), boil in a water bath for 15 min, then immediately transfer to an ice water bath for 5 min; add 1 mL Ehrlich reagent (0.8 g p-dimethylaminobenzaldehyde dissolved in 15 mL concentrated hydrochloric acid and 15 mL anhydrous ethanol), dilute with 3 mL anhydrous ethanol, incubate at room temperature for 20 min for color development, and measure its absorbance at 540 nm, using 0.5 mg / mL dipotassium glycyrrhizate solution as a positive control.

[0066] The inhibition rate of hyaluronidase is calculated using the following formula: Hyaluronidase inhibition rate = {[(AB)-(CD)] / (AB)}×100%.

[0067] Where: A - absorbance of the control solution (using acetate buffer solution instead of the sample solution); B - absorbance of the control blank solution (using acetate buffer solution instead of the sample solution and enzyme solution); C - absorbance of the sample solution; D - absorbance of the sample blank solution (using acetate buffer solution instead of the enzyme solution).

[0068] The specific test results are shown in Table 3 below: Table 3

[0069] The results show that the compositions prepared in Examples 1-3 of this invention have better soothing and anti-allergic properties compared with Comparative Examples 1-3.

[0070] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A moisturizing, repairing, and anti-allergic composition containing Cornus officinalis extract, characterized in that, It includes the following ingredients: Cornus officinalis extract, beetroot extract, sea buckthorn fruit extract, Chrysanthemum indicum extract, and carboxymethyl deacetylated chitosan.

2. The composition according to claim 1, characterized in that, The mass ratio of Cornus officinalis extract, Chrysanthemum indicum extract and carboxymethyl deacetylated chitosan is 4-6:2-4:0.1-1.

5.

3. The composition according to claim 2, characterized in that, The mass ratio of the Cornus officinalis extract, Chrysanthemum indicum extract, and carboxymethyl deacetylated chitosan is 4-6:2-4:0.5-1.

5.

4. The composition according to claim 1, characterized in that, The preparation method of the *Cyprinus sarmentosus* fruit extract includes the following steps: after crushing the *Cyprinus sarmentosus* seeds, add 50-60 wt% ethanol solution, extract once at 50-60℃ for 10-30 min under ultrasonic assistance, then add ethanol to the solution to an ethanol concentration of 70-80 wt%, extract a second time at 35-45℃ for 10-30 min under ultrasonic assistance, combine the extracts, purify with macroporous resin and dry to obtain the extract.

5. The composition according to claim 1, characterized in that, The preparation method of Shennong Xiangju extract includes the following steps: fresh Shennong Xiangju flowers are crushed and mixed with water, then hydrolyzed by cellulase and pectinase, extracted with 55-65wt% ethanol, separated by nanofiltration membrane to remove macromolecular volatile oils, and then dried to obtain the extract.

6. The composition according to claim 1, characterized in that, The product comprises the following ingredients by weight: 8-12 parts Cornus officinalis extract, 1-10 parts Beetroot extract, 1-10 parts Prickly pear extract, 4-8 parts Chrysanthemum indicum extract, and 1-3 parts Carboxymethyl deacetylated chitosan.

7. The composition according to claim 6, characterized in that, The product comprises the following ingredients by weight: 10 parts Cornus officinalis extract, 6 parts Beetroot extract, 4 parts Prickly pear extract, 6 parts Chrysanthemum indicum extract and 2 parts carboxymethyl deacetylated chitosan.

8. A method for preparing the composition according to any one of claims 1-7, characterized in that, The process includes the following steps: mixing the raw materials.

9. The use of the composition according to any one of claims 1-7 in the preparation of cosmetics with moisturizing, repairing and anti-allergic effects.

10. The application according to claim 9, characterized in that, The cosmetics include facial cleansers, toners, essences, serums, face creams, face masks, eye creams, body lotions, body oils, hand creams, and foot creams.

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