A method for preparing tea fiber from Hainan partridge tea

Abstract

This invention relates to the field of functional regenerated cellulose fiber technology, specifically to a method for preparing tea fiber using Hainan partridge tea, comprising the following steps: taking fresh partridge tea leaves, removing stems, washing, air-drying, and pulverizing to obtain fine tea powder; adding phosphate buffer to the fine tea powder, then adding a compound enzyme, stirring, followed by enzymatic hydrolysis, enzyme inactivation, filtration, discarding the filter residue, and collecting the filtrate; homogenizing the filtrate obtained in step S2 to obtain nano-slurry, adding sodium alginate, stirring, and obtaining nano-slurry; taking bamboo pulp, pulverizing, and drying to obtain treated bamboo pulp; mixing the nano-slurry with the treated bamboo pulp, then transferring the mixture to a maturation tank, and maturing at 45-50℃ for 15-20 hours to obtain a matured blend; spinning the matured blend, washing, drying, and cutting to obtain a tea fiber prepared from Hainan partridge tea. The tea fiber prepared in this application has high activity retention rate, good antibacterial properties, and high water washing stability.

Description

Technical Field

[0001] This invention relates to the field of functional regenerated cellulose fiber technology, specifically to a method for preparing tea fiber using Hainan partridge tea. Background Technology

[0002] Hainan partridge tea is a unique wild medicinal plant native to Hainan. Traditionally, it is believed to have effects such as clearing heat and detoxifying, reducing inflammation and promoting bile secretion, aiding digestion and relieving greasiness, and dispelling wind and releasing the exterior. Modern pharmacological research shows that partridge tea is rich in high concentrations of flavonoids (vitexin, isovitexin), polyphenols, plant polysaccharides, lupeol, squalene, and other active substances, exhibiting outstanding antioxidant, antibacterial, and anti-inflammatory properties. Furthermore, it is caffeine-free and gentle, without causing irritation. Currently, the process of precisely compounding partridge tea with bamboo pulp using nano-pulping to prepare functional fibers represents a significant technological gap and industrialization potential within the industry.

[0003] Existing tea fiber is mostly made from green tea, black tea, and tea residue, and is prepared using high-temperature boiling and simple physical mixing of powders, which has many technical shortcomings: 1. The raw materials lack regional characteristics and do not utilize the unique medicinal and edible resources and special active components of Hainan's authentic partridge tea; 2. High-temperature processing damages heat-sensitive components such as flavonoids and polyphenols, resulting in low activity retention and significantly reduced functionality; 3. The mixture is only roughly mixed at the micron level, making it extremely easy to agglomerate and resulting in uneven distribution of fiber functions; 4. The active ingredients are only physically attached and have no molecular bonding, resulting in extremely poor water resistance and loss of function after repeated washing. 5. The production process often uses chemical cross-linking agents and organic solvents, which are not environmentally friendly and do not conform to the development trend of green, low-carbon and biodegradable materials. Summary of the Invention

[0004] To address the aforementioned issues, the purpose of this application is to provide a method for preparing tea fiber using Hainan partridge tea. The method is simple, utilizing Hainan's unique partridge tea resources, and combining low-temperature enzymatic hydrolysis, nano-slurry, and bamboo pulp to prepare functional fibers. The prepared tea fiber has high activity retention rate, good antibacterial properties, and high water washability.

[0005] To achieve the above objectives, this application provides a method for preparing tea fiber from Hainan partridge tea, comprising the following steps: S1. Take fresh partridge tea leaves, remove the stems, wash them, air dry them, and grind them into fine tea powder; S2. Add phosphate buffer to the fine tea powder, then add compound enzyme, stir, enzymatically hydrolyze, inactivate enzyme, filter, discard the filter residue, and collect the filtrate. S3. Homogenize the filtrate obtained in step S2 to obtain a nano slurry, add sodium alginate, stir, and obtain the nano slurry. S4. Take bamboo pulp, crush it, and dry it to obtain processed bamboo pulp; S5. Take the nano-slurry and mix it with the treated bamboo pulp. Then transfer the mixture to a maturation tank and let it stand at 45-50℃ for 15-20 hours to obtain the maturation blend. S6. Spin the matured blend, wash, dry, and cut to obtain a tea fiber prepared from Hainan partridge tea.

[0006] Furthermore, the air-drying process involves air-drying at 40-45℃ for 7-8 hours.

[0007] Furthermore, the pulverization described in step S1 is performed at a speed of 4000-6000 rpm until it passes through a 90-100 mesh sieve.

[0008] Furthermore, the phosphate buffer solution has a pH of 5.8-6.2, and its ratio to the fine tea powder is 17-19 mL:1 g.

[0009] Furthermore, the complex enzyme is composed of cellulase and hemicellulase, with a mass ratio of cellulase to hemicellulase of 2-3:1, and a mass ratio of the complex enzyme to tea powder of 1-2:100.

[0010] Furthermore, the enzymatic hydrolysis is performed in a constant temperature water bath at 45-50℃ for 2-3 hours.

[0011] Furthermore, the enzyme inactivation is carried out at 75-85℃ for 10-15 minutes.

[0012] Furthermore, the homogenization process involves a first-stage pressure of 80-100 MPa and a second-stage pressure of 20-30 MPa, with the homogenization cycle repeated 4-5 times.

[0013] Furthermore, the sodium alginate is used in an amount of 0.4-0.5% of the mass of the nano-slurry.

[0014] Furthermore, the pulverization described in step S4 is performed to a particle size of 100-110 mesh.

[0015] Furthermore, the sun-drying process continues until the moisture content reaches 10-13%.

[0016] Furthermore, the mass ratio of the mixed nano-slurry to the treated bamboo pulp is 1:8-10.

[0017] Furthermore, the stirring is carried out at 50-55°C and a speed of 700-800 rpm for 50-60 minutes.

[0018] Furthermore, the spinning process involves a spinning temperature of 55-60℃, a pore size of 0.1-0.2mm, and a stretching ratio of 2.5-3 times.

[0019] In summary, this application has the following beneficial effects: 1. Raw material innovation: For the first time, Hainan partridge tea is exclusively used, which is different from all ordinary tea fiber and has its own regional scarcity and traditional medicinal value.

[0020] 2. High component retention: Low-temperature enzymatic hydrolysis avoids high-temperature damage, resulting in a high retention rate of flavonoids and polyphenols, far exceeding that of traditional high-temperature processes.

[0021] 3. Precise nano-dispersion: 50-200nm nano-slurry achieves uniform molecular-level distribution within bamboo pulp, with no agglomeration and consistent function.

[0022] 4. Long-lasting and stable function: Hydrogen bonding fixation results in high function retention after washing, far exceeding that of traditional physically blended fibers.

[0023] 5. Eco-friendly: Natural buffer system and sodium alginate stabilizer, no toxic chemical additives, easy-to-treat production wastewater, and biodegradable finished products.

[0024] 6. Wide range of performance and applications: It has strong antibacterial effect and good spinnability, and can be used in underwear and home textiles, medical dressings, skin care fabrics, biodegradable packaging, etc.

[0025] 7. Sustainable Industry: It drives the entire industrial chain of Hainan partridge tea planting, deep processing, and functional textiles, and helps to increase the value of tropical resources. Detailed Implementation

[0026] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the embodiments of this application. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this invention.

[0027] The raw materials involved in the specific embodiments of this application are analytical grade. In addition, the cellulase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., catalog number: S10042, activity: 400u / mg; the hemicellulase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., catalog number: V30137, activity: 200u / mg; the bamboo pulp has α-cellulose ≥94.0%, ash content (725℃) ≤0.16%, whiteness ≥74%, and alkali absorption value ≥450%.

[0028] Example 1 A method for preparing tea fiber using Hainan partridge tea includes the following steps: S1. Select 100g of fresh partridge tea leaves from Dongshanling, Wanning. Process the leaves within 24 hours of picking, remove the stems by hand, wash off surface impurities with pure water, spread them on a clean tray, and air-dry them at 42℃ for 7 hours. Grind the air-dried tea leaves into powder using a toothed disc high-speed universal grinder (5000rpm) and pass them through a 90-mesh sieve to obtain fine tea powder. S2. Place the fine tea powder obtained in step S1 into a reaction vessel, add 1800 mL of pH 6.0 phosphate buffer, and then add 1 g of compound enzyme (the mass ratio of cellulase to hemicellulase is 2:1). Stir at 100 rpm for 30 minutes, and then enzymatically hydrolyze in a 48℃ constant temperature water bath for 2.5 hours, stirring once every 30 minutes during the process. After the enzymatic hydrolysis is completed, raise the temperature to 80℃ and keep it at that temperature for 10 minutes. Vacuum filter through a 200-mesh filter cloth, discard the filter residue, and collect the filtrate. S3. Transfer the filtrate obtained in step S2 to a high-pressure homogenizer for high-pressure homogenization. Set the first pressure to 90 MPa and the second pressure to 25 MPa. Homogenize for 4 cycles to obtain nano slurry. Add 7.2 g of sodium alginate to the slurry and stir at 600 rpm for 30 min at room temperature to obtain a stable nano slurry. S4. Take bamboo pulp, grind it to 110 mesh using a hammer mill, and dry it until the moisture content is 13%. S5. Take 180g of the nano slurry obtained in step S3 and 1800g of the bamboo pulp obtained in step S4 and put them into a mixing tank. Mix them at 52℃ and 700rpm for 50min. Then transfer the mixture to a maturation tank and let it stand at 48℃ for 15h to obtain the maturation blend. S6. The matured blend is fed into a spinning machine at a spinning temperature of 58°C. A spinneret with a 0.1mm aperture is used for spinning, and the stretch ratio is 2.8 times. The blend is washed, dried, and cut into short fibers of 45mm in length using a cutting machine to obtain a tea fiber prepared from Hainan partridge tea.

[0029] Example 2 A method for preparing tea fiber using Hainan partridge tea includes the following steps: S1. Select 100g of fresh partridge tea leaves from Dongshanling, Wanning. Process the leaves within 24 hours of picking, remove the stems by hand, wash off surface impurities with pure water, spread them on a clean tray, and air-dry them at 42℃ for 7 hours. Grind the air-dried tea leaves into powder using a toothed disc high-speed universal grinder (5000rpm) and pass them through a 90-mesh sieve to obtain fine tea powder. S2. Place the fine tea powder obtained in step S1 into a reaction vessel, add 1800 mL of pH 6.0 phosphate buffer, and then add 1 g of compound enzyme (the mass ratio of cellulase to hemicellulase is 2:1). Stir at 100 rpm for 30 minutes, and then enzymatically hydrolyze in a 48℃ constant temperature water bath for 2.5 hours, stirring once every 30 minutes during the process. After the enzymatic hydrolysis is completed, raise the temperature to 80℃ and keep it at that temperature for 10 minutes. Vacuum filter through a 200-mesh filter cloth, discard the filter residue, and collect the filtrate. S3. Transfer the filtrate obtained in step S2 to a high-pressure homogenizer for high-pressure homogenization. Set the first pressure to 80 MPa and the second pressure to 20 MPa. Homogenize for 5 cycles to obtain nano slurry. Add 7.2 g of sodium alginate to the slurry and stir at 600 rpm for 30 min at room temperature to obtain a stable nano slurry. S4. Take bamboo pulp, grind it to 110 mesh using a hammer mill, and dry it until the moisture content is 13%. S5. Take 180g of the nano slurry obtained in step S3 and 1440g of the bamboo pulp obtained in step S4 and put them into a mixing tank. Mix them at 52℃ and 700rpm for 50min. Then transfer the mixture to a maturation tank and let it stand at 48℃ for 15h to obtain the maturation blend. S6. The matured blend is fed into a spinning machine at a spinning temperature of 58°C. A spinneret with a 0.1mm aperture is used for spinning, and the stretch ratio is 2.8 times. The blend is washed, dried, and cut into short fibers of 45mm in length using a cutting machine to obtain a tea fiber prepared from Hainan partridge tea.

[0030] Example 3 A method for preparing tea fiber using Hainan partridge tea includes the following steps: S1. Select 100g of fresh partridge tea leaves from Dongshanling, Wanning. Process the leaves within 24 hours of picking, remove the stems by hand, wash off surface impurities with pure water, spread them on a clean tray, and air-dry them at 42℃ for 7 hours. Grind the air-dried tea leaves into powder using a toothed disc high-speed universal grinder (5000rpm) and pass them through a 90-mesh sieve to obtain fine tea powder. S2. Place the tea powder obtained in step S1 into a reaction vessel, add 1800 mL of pH 6.0 phosphate buffer, and then add 1 g of compound enzyme (the mass ratio of cellulase to hemicellulase is 2:1). Stir at 100 rpm for 30 minutes, and then enzymatically hydrolyze in a 45℃ constant temperature water bath for 3 hours, stirring once every 30 minutes during the process. After the enzymatic hydrolysis is completed, raise the temperature to 80℃ and keep it at that temperature for 10 minutes. Vacuum filter through a 200-mesh filter cloth, discard the filter residue, and collect the filtrate. S3. Transfer the filtrate obtained in step S2 to a high-pressure homogenizer for high-pressure homogenization. Set the first pressure to 90 MPa and the second pressure to 25 MPa. Homogenize for 4 cycles to obtain nano slurry. Add 7.2 g of sodium alginate to the slurry and stir at 600 rpm for 30 min at room temperature to obtain a stable nano slurry. S4. Take bamboo pulp, grind it to 110 mesh using a hammer mill, and dry it until the moisture content is 13%. S5. Take 180g of the nano slurry obtained in step S3 and 1800g of the bamboo pulp obtained in step S4 and put them into a mixing tank. Mix them at 52℃ and 700rpm for 50min. Then transfer the mixture to a maturation tank and let it stand at 48℃ for 15h to obtain the maturation blend. S6. The matured blend is fed into a spinning machine at a spinning temperature of 55°C. A spinneret with a 0.1mm aperture is used for spinning, and the stretch ratio is 2.5 times. The product is then washed, dried, and cut into short fibers of 45mm length using a cutting machine to obtain a tea fiber prepared from Hainan partridge tea.

[0031] Compare with Example 1 Compared with Example 1, this comparative example differs in that it uses a traditional process to prepare tea fiber. The preparation method of tea fiber using Hainan partridge tea in this comparative example includes the following steps: S1. Select 100g of fresh partridge tea leaves from Dongshanling, Wanning. Process the leaves within 24 hours of picking, remove the stems by hand, wash off surface impurities with pure water, spread them on a clean tray, and air-dry them at 42℃ for 7 hours. Grind the air-dried tea leaves into powder using a toothed disc high-speed universal grinder (5000rpm) and pass them through a 90-mesh sieve to obtain fine tea powder. S2. Take bamboo pulp, grind it to 110 mesh using a hammer mill, and dry it until the moisture content is 13%. S3. Take 100g of the fine tea powder obtained in step S1 and 1000g of the bamboo pulp obtained in step S4 and put them into a mixing kettle. Mix them at 80℃ and 700rpm for 50min to obtain a blend. S4. The blend is fed into a spinning machine at a spinning temperature of 58°C. A spinneret with a 0.1mm aperture is used for spinning, and the stretch ratio is 2.8 times. The blend is washed, dried, and cut into short fibers of 45mm in length using a cutting machine to obtain a tea fiber prepared from Hainan partridge tea.

[0032] Performance testing Functional tests were conducted on the tea fibers prepared in Examples 1-3 and Control Example 1.

[0033] Flavonoid activity retention rate: Take 1.0g of tea fiber, cut it into 2mm pieces, place it in a Soxhlet extractor, add 70% ethanol (solid-liquid ratio 1g:20mL), reflux extract at 90℃ for 2h, concentrate the extract under reduced pressure to dryness, and make up to 50mL with 70% ethanol. Shake well and set aside. Take an equal amount of raw partridge tea ultrafine powder and process it in the same way to obtain a control solution. Measure the flavonoid content using spectrophotometry and calculate the retention rate. Flavonoid activity retention rate (%) = total flavonoid content in finished fiber / total flavonoid content in raw tea leaves × 100%; Escherichia coli inhibition rate: The Escherichia coli strain was obtained from the Shanghai Institute of Industrial Microbiology, number: JI23657. The strain was inoculated into nutrient broth medium and cultured at 37°C with shaking for 18-24 hours. The culture was then diluted with sterile physiological saline to a bacterial suspension concentration of 3×10⁻⁶. 5 CFU / mL, cut tea fibers into approximately 5mm long fragments, weigh 0.75g. Negative control group: standard cotton cloth, same weight, untreated. Place the sample in a 250mL Erlenmeyer flask, add 70mL PBS buffer (0.03mol / L, pH 7.2) and 5mL bacterial suspension, tighten the stopper, and incubate at 24℃ and 150rpm for 18h with shaking. After shaking, take 1mL of the mixture and dilute to 10. -6 Spread 0.1 mL of the sample onto a nutrient agar plate and incubate at 37°C for 24 hours. Count the colonies (CFU / mL) and calculate the inhibition rate. Inhibition rate (%) = C 对照 -C 试样 / C 对照 ×100%, where C 对照 The viable bacterial concentration (CFU / mL) of the positive control sample, C 试样 The concentration of viable bacteria after sample shaking; Functional retention rate after 50 washes: Following GB / T 8629-2017 "Home Washing and Drying Procedures for Textile Testing", the fibers were repeatedly washed 50 times. The washed fibers were then tested using the aforementioned method for E. coli inhibition rate. The retention rate was calculated as follows: Functional retention rate after 50 washes (%) = Functional value after washing / Initial functional value before washing × 100%. The results are shown in Table 1. Table 1

[0034] As shown in Table 1, the tea fiber prepared in this application embodiment is significantly superior to the existing traditional tea fiber process in terms of activity retention, antibacterial properties, and water washing stability, demonstrating clear technological progress.

[0035] The above description is merely an example and illustration of the concept of this application. Those skilled in the art can make various modifications or additions to the specific embodiments described or use similar methods to replace them, as long as they do not deviate from the inventive concept or exceed the scope defined in the claims, they should all fall within the protection scope of this application.

Claims

1. A method for preparing tea fiber using Hainan partridge tea, characterized in that, Includes the following steps: S1. Take fresh partridge tea leaves, remove the stems, wash them, air dry them, and grind them into fine tea powder; S2. Add phosphate buffer to the fine tea powder, then add compound enzyme, stir, enzymatically hydrolyze, inactivate enzyme, filter, discard the filter residue, and collect the filtrate. S3. Homogenize the filtrate obtained in step S2 to obtain a nano slurry, add sodium alginate, stir, and obtain the nano slurry. S4. Take bamboo pulp, crush it, and dry it to obtain processed bamboo pulp; S5. Take the nano-slurry and mix it with the treated bamboo pulp. Then transfer the mixture to a maturation tank and let it stand at 45-50℃ for 15-20 hours to obtain the maturation blend. S6. Spin the matured blend, wash, dry, and cut to obtain a tea fiber prepared from Hainan partridge tea.

2. The method for preparing tea fiber using Hainan partridge tea according to claim 1, characterized in that, The phosphate buffer solution has a pH of 5.8-6.2, and its ratio to the fine tea powder is 17-19 mL:1 g.

3. The method for preparing tea fiber using Hainan partridge tea according to claim 1, characterized in that, The complex enzyme is composed of cellulase and hemicellulase, with a mass ratio of cellulase to hemicellulase of 2-3:1, and a mass ratio of the complex enzyme to tea powder of 1-2:

100.

4. The method for preparing tea fiber using Hainan partridge tea according to claim 1, characterized in that, The enzymatic hydrolysis is carried out in a constant temperature water bath at 45-50℃ for 2-3 hours.

5. The method for preparing tea fiber using Hainan partridge tea according to claim 1, characterized in that, The enzyme inactivation is carried out at 75-85℃ for 10-15 minutes.

6. The method for preparing tea fiber from Hainan partridge tea according to claim 1, characterized in that, The homogenization process involves a first-stage pressure of 80-100 MPa and a second-stage pressure of 20-30 MPa, with the homogenization cycle repeated 4-5 times.

7. The method for preparing tea fiber using Hainan partridge tea according to claim 1, characterized in that, The sodium alginate is used at a rate of 0.4-0.5% of the mass of the nano-slurry.

8. The method for preparing tea fiber using Hainan partridge tea according to claim 1, characterized in that, The process involves drying the product until its moisture content reaches 10-13%.

9. A method for preparing tea fiber from Hainan partridge tea according to claim 1, characterized in that, The mass ratio of the nano-slurry to the treated bamboo pulp in the mixture is 1:8-10.

10. A method for preparing tea fiber from Hainan partridge tea according to claim 1, characterized in that, The spinning process involves a spinning temperature of 55-60℃, an aperture of 0.1-0.2mm, and a draw ratio of 2.5-3 times.

Images