A method for slaughtering large yellow croaker

Abstract

A method for slaughtering large yellow croaker, which is completed through temporary cultivation, fish selection, loss of consciousness in saturated CO2 environment at 20 DEG C, bloodletting, removal of viscera and gills, ion water flushing, packaging and cold storage. The method for losing consciousness in saturated CO2 environment is applied to slaughtering, which can effectively reduce the adverse effects of fish muscle activity and ATP degradation in fish meat caused by slaughtering stress on the texture, color and shelf life of fish fillets. Meanwhile, the animal welfare is greatly improved, and it is a valuable slaughtering method which can be popularized.

Description

Technical Field

[0001] This invention relates to a method for processing aquatic products, particularly a method for slaughtering large yellow croaker, which can better extend the shelf life of spinach. Background Technology

[0002] Large yellow croaker ( Larimichthys Crocea The large yellow croaker (Croatia spp.) is distributed along the southeastern coast of China. Currently, it is considered one of the most commercially and economically valuable marine fish in China, prized for its delicious taste, high nutritional value, and significant market potential. Slaughtering is a critical point in aquaculture that can seriously harm fish welfare. Slaughtering methods have a certain impact on the biochemical processes of fish muscles after death; slaughter stress increases muscle activity and ATP degradation, thus adversely affecting the texture, color, and shelf life of fish fillets. The European Food Safety Authority (EFSA) states that slaughter should immediately induce coma in fish to reduce stress and improve animal welfare. There is an urgent need for a slaughtering method that improves animal welfare and facilitates subsequent cold chain preservation. Summary of the Invention

[0003] The purpose of this invention is to kill large yellow croaker by inducing unconsciousness in a CO2 environment. This method can significantly improve animal welfare and reduce the stress response of large yellow croaker.

[0004] A method for slaughtering large yellow croaker, characterized by the following steps: This invention is achieved through the following technical steps: This invention is achieved through the following technical steps: Temporary holding: After purchasing, large yellow croakers are temporarily held in a 500 L culture tank without food for 24 hours. During the temporary holding period, the temperature is 20~22℃, the salinity is 4~6 mg / L, and the dissolved oxygen is 16‰. Choose large yellow croakers that are similar in size and breathing rate; Loss of consciousness in a saturated CO2 environment at 20 °C; After the large yellow croaker lost consciousness, it was bled and rinsed with deionized water. Remove the internal organs and gills, and rinse with deionized water; The whole fish were packaged in plastic bags and stored in a refrigerator at 4±1℃.

[0005] Preferably, in the temporary rearing, the large yellow croaker is temporarily reared in a 500 L rearing tank for 24 hours without food after purchase. The temperature during the temporary rearing period is 21 ℃, the salinity is 5 mg / L, and the dissolved oxygen is 16‰.

[0006] Preferably, the large yellow croaker loses consciousness in a saturated CO2 environment at 20 ℃. In a 100% CO2 environment at 20 ℃, the large yellow croaker stops eye movement and does not respond to external stimuli.

[0007] This invention applies the method of inducing unconsciousness in a saturated CO2 environment to slaughter, effectively reducing the adverse effects of slaughter stress on fish muscle activity and ATP degradation in fish meat, which negatively impacts the texture, color, and shelf life of fish fillets. Simultaneously, it significantly improves animal welfare and represents a valuable and scalable slaughter method. Detailed Implementation

[0008] To make the operational process and creative features of this invention easy to understand, and to fully disclose a method for preserving spinach, the invention is further described below with reference to specific embodiments.

[0009] Example (1) Temporary holding: After purchasing the large yellow croaker, it is temporarily held in a 500 L breeding box for 24 hours without food. The temperature during the temporary holding period is 21℃, the salinity is 5 mg / L, and the dissolved oxygen is 16‰; (2) Select large yellow croaker with similar body size and respiratory frequency; (3) Place the large yellow croaker in a 20℃ saturated 100% CO2 environment until it loses consciousness, the eyeballs stop moving, and it does not respond to external stimuli; (4) Bleed the large yellow croaker that has lost consciousness and rinse it with deionized water; (5) Remove the internal organs and gills and rinse it with deionized water; (6) Pack the whole fish in plastic bags and store them in a refrigerator at 4±1℃.

[0010] In terms of physiological stress, blood glucose (GLU), lactate dehydrogenase (LDH), and catalase (CAT) levels were significantly lower than in other slaughter methods. P <0.05). Superoxide dismutase (SOD) and glutathione peroxidase (GPx) analyses showed that animals in a 100% CO2 saturated environment, even after loss of consciousness, maintained good antioxidant enzyme activity during short-term post-mortem refrigeration, while preserving protein stability (lowest carbonyl content and surface hydrophobicity) and reducing lipid oxidation. Furthermore, pH and water-holding capacity (WHC) analyses indicated that animals in a 100% CO2 saturated environment, even after loss of consciousness, exhibited better muscle quality. Most importantly, the slaughter method of this invention significantly improves animal welfare.

[0011] The above embodiments are merely illustrative of the design principles and uses of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A method for slaughtering large yellow croaker, characterized by the following steps: This invention is achieved through the following technical steps: Temporary holding: After purchasing, large yellow croakers are temporarily held in a 500 L culture tank without food for 24 hours. During the temporary holding period, the temperature is 20~22 ℃, the salinity is 4~6 mg / L, and the dissolved oxygen is 16‰. Choose large yellow croakers that are similar in size and breathing rate; Loss of consciousness in a saturated CO2 environment at 20 °C; Bleed the unconscious large yellow croaker and rinse it with deionized water; Remove the internal organs and gills, and rinse with deionized water; The whole fish were packaged in plastic bags and stored in a refrigerator at 4±1℃.

2. The method for slaughtering large yellow croaker according to claim 1, characterized in that: The aforementioned temporary holding involved fasting and holding the large yellow croaker in a 500 L rearing tank for 24 hours after purchase. The temperature during the temporary holding period was 21℃, the salinity was 5 mg / L, and the dissolved oxygen was 16‰.

3. The method for slaughtering large yellow croaker according to claim 1, characterized in that: The large yellow croaker lost consciousness in a saturated CO2 environment at 20°C. In a 100% CO2 environment at 20°C, the large yellow croaker stopped moving its eyes and did not respond to external stimuli.