Anti-wrinkle firming multi-component serum and preparation method thereof

Abstract

The application belongs to the technical field of cosmetics, and provides an anti-wrinkle firming multi-component essence and a preparation method thereof, which comprises the following components: embryo extract, anti-wrinkle firming composition, moisturizing system, soothing ingredient, antioxidant ingredient, thickening agent, chelating agent, pH regulator, preservative, fragrance, and the balance is water. The multi-component essence of the application takes the embryo extract as the core, cooperates with various anti-wrinkle ingredients, realizes the triple anti-wrinkle mechanism of "promoting synthesis-inhibiting degradation-repairing barrier", and the elastase inhibition rate is greater than or equal to 85.5% under the concentration of 10%, which is significantly better than the traditional single-component product; on the basis of anti-wrinkle firming, the essence has the effects of soothing, moisturizing and antioxidation, and meets the multiple skin care needs in the anti-wrinkle process; the essence is free of alcohol and strong acidic ingredients, and is verified by zebrafish test and skin irritation test to be suitable for sensitive skin; the conventional cosmetic production equipment is adopted, the step-by-step preparation process is simple and controllable, and the essence is suitable for large-scale production.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an anti-wrinkle and firming multi-component serum and its preparation method. Background Technology

[0002] With age and environmental factors, collagen in the dermis is lost and elastic fibers break down, leading to wrinkles and decreased firmness. Anti-wrinkle and firming effects have become a core demand in the skincare industry. However, current anti-wrinkle products have the following limitations:

[0003] Single efficacy: Most products rely on a single ingredient (such as retinol or hexapeptide-8), which can only improve surface dynamic wrinkles and cannot solve the problem of aging of the dermal structure. Moreover, they require long-term use to see results, resulting in poor consumer compliance.

[0004] Highly irritating: In pursuit of immediate results, some products add high concentrations of acids or alcohol, which can cause skin redness and peeling, making them unsuitable for sensitive skin.

[0005] Insufficient ingredient synergy: Anti-wrinkle ingredients have poor compatibility with moisturizing and soothing ingredients, which can easily lead to problems such as layering and degradation of active ingredients, affecting product stability.

[0006] Embryonic extract, a high-end raw material rich in natural active substances, contains thousands of proteins, small peptides, and active small molecules, exhibiting unique advantages in promoting fibroblast activity and stimulating collagen synthesis. However, current technologies have not yet used it as a core anti-wrinkle ingredient to construct an integrated anti-wrinkle and firming system encompassing raw materials, formulation, and processing. Furthermore, its synergistic mechanism with other anti-wrinkle ingredients has not been fully explored. Therefore, developing a multi-component serum with embryonic extract as its core, offering high efficacy, gentle safety, and stable controllability in anti-wrinkle treatment, is of great significance. Summary of the Invention

[0007] The purpose of this invention is to provide an anti-wrinkle and firming multi-component serum and its preparation method. It uses embryo extract as the core, synergistically combines multiple anti-wrinkle ingredients, and adopts a technical approach of "core ingredient-led, multi-component synergistic, and process optimization" to achieve a three-dimensional anti-wrinkle and firming effect from the dermis to the epidermis.

[0008] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0009] This invention provides an anti-wrinkle and firming multi-component essence, comprising the following components by mass percentage: 0.285-0.5% embryo extract, 0.8-1.5% anti-wrinkle and firming composition, 10-15% moisturizing system, 0.15-0.3% soothing ingredient, 0.03-0.1% antioxidant ingredient, 0.2-0.5% thickener, 0.03-0.08% chelating agent, 0.3-0.6% pH adjuster, 0.05-0.1% preservative, 0.05-0.1% fragrance, and the balance being water.

[0010] Preferably, the embryo extract is derived from sheep embryo tissue, wherein the proportion of small molecule active peptides is ≥30%, and the protein molecular weight distribution is 5000~50000Da.

[0011] Embryo extract is the core component of the multi-component essence of this invention. It is derived from sheep embryo tissue and is prepared through low-temperature enzymatic hydrolysis and ultrafiltration purification. Its core functions include:

[0012] Promote collagen synthesis: Small molecule active peptides (such as insulin-like growth factor and fibroblast growth factor) act directly on dermal fibroblasts, stimulating the synthesis of type I and type III collagen and elastin, and increasing the density of the dermal layer of the skin.

[0013] Inhibition of elastase: Specific protein components can competitively inhibit elastase activity, reducing the degradation of elastic fibers;

[0014] Improve the cellular microenvironment: By regulating the composition of the extracellular matrix (ECM), it promotes the repair of damaged tissue, enhances the skin barrier function, and indirectly improves skin firmness;

[0015] Synergistic effect: It forms a "peptide-protein" complex system with palmitoyl tripeptide-1, amplifying the anti-wrinkle signaling pathway and improving the anti-wrinkle effect compared with single ingredients.

[0016] Preferably, the anti-wrinkle and firming composition comprises Bifida ferment lysate, panthenol, squalane, jojoba seed oil, and palmitoyl tripeptide-1, wherein the mass ratio of Bifida ferment lysate, panthenol, squalane, jojoba seed oil, and palmitoyl tripeptide-1 is 0.1–0.2:0.8–1.2:0.1–0.6:0.4–0.6:0.0005–0.001;

[0017] In the anti-wrinkle and firming composition, the bifida ferment lysate is rich in B vitamins and nucleotides, which activates autophagy, promotes the metabolism of aging cells, and synergistically enhances fibroblast activity with embryo extract.

[0018] After panthenol is converted into pantothenic acid (vitamin B5), it participates in the synthesis of coenzyme A, promotes epithelial cell regeneration, and enhances skin elasticity;

[0019] Squalane and jojoba seed oil mimic the skin's natural lipids, repairing barrier defects and reducing moisture loss caused by anti-wrinkle ingredients, while also providing a lipid carrier for embryo extract to promote transdermal absorption.

[0020] Palmitoyl tripeptide-1, as a signal peptide, further stimulates collagen synthesis, forming a "dual stimulation" mechanism with embryonic extract.

[0021] The moisturizing system comprises glycerin, propylene glycol, 1,3-propanediol, 1,2-hexanediol, sodium hyaluronate, and acetylated sodium hyaluronate, wherein the mass ratio of glycerin, propylene glycol, 1,3-propanediol, 1,2-hexanediol, sodium hyaluronate, and acetylated sodium hyaluronate is 5–8:2–4:0.5–1:0.2–0.5:0.01–0.03:0.02–0.03;

[0022] In the moisturizing system, glycerin increases the water content of the stratum corneum through hygroscopic action and forms a hydration film with sodium hyaluronate-like ingredients, reducing transepidermal water loss; 1,3-propanediol and 1,2-hexanediol synergistically enhance the stability of the system and improve the skin feel.

[0023] The soothing ingredients include bisabolol, chamomile extract, and rosemary leaf extract, wherein the mass ratio of bisabolol, chamomile extract, and rosemary extract is 0.15–0.25:0.002–0.003:0.002–0.003;

[0024] Among the soothing ingredients, bisabolol exerts its anti-inflammatory effect by inhibiting the release of inflammatory factors (such as IL-6), while chamomile extract and rosemary leaf extract help relieve skin discomfort.

[0025] The antioxidant components include sodium polyglutamate and tocopheryl acetate, wherein the mass ratio of sodium polyglutamate to tocopheryl acetate is 0.02-0.06:0.01-0.04.

[0026] Among antioxidant ingredients, tocopheryl acetate has a good ability to scavenge free radicals and can protect the active peptides in embryo extracts from oxidation; sodium polyglutamate can enhance the system's moisturizing ability while delaying the release of active ingredients.

[0027] Preferably, the thickener is an acrylate or a C10-C30 alkanol acrylate crosslinked polymer;

[0028] This invention constructs a three-dimensional network structure using acrylate crosslinking polymers, which stabilizes water-oil emulsion droplets (particle size 50-100 nm), prevents stratification, and extends the product shelf life to 24 months.

[0029] The chelating agent is disodium EDTA;

[0030] The pH adjuster includes arginine and citric acid, wherein the mass ratio of arginine to citric acid is 0.3–0.5:0.03–0.06.

[0031] The preservatives include capryloyl hydroxamic acid and phenoxyethanol, wherein the mass ratio of capryloyl hydroxamic acid to phenoxyethanol is 0.05–0.07:0.0005–0.002;

[0032] The fragrance is a flavoring.

[0033] This invention uses a low-irritant preservative combination (capryloyl hydroxamic acid + phenoxyethanol) and controls the concentration, making it suitable for various skin types.

[0034] This invention also provides a method for preparing embryonic extract, used in the multi-component essence provided by this invention, comprising the following steps:

[0035] S1. Sheep embryonic tissue was freeze-dried, pulverized, and mixed with water. The pH was adjusted with phosphate buffer.

[0036] S2. After adding the complex protease for enzymatic hydrolysis, the enzyme activity is inactivated by water bath.

[0037] S3. Centrifuge and filter the supernatant, then freeze-dry the filtrate to obtain the embryo extract.

[0038] Preferably, in step S1, the solid-liquid ratio of sheep embryonic tissue to water is 1:8 to 12, and the pH is adjusted to 6.5 to 7.0.

[0039] In step S2, the mass of the complex protease is 1-3% of the mass of the sheep embryonic tissue. The complex protease includes trypsin and papain, wherein the mass ratio of trypsin and papain is 1:1.

[0040] In step S2, the enzymatic hydrolysis temperature is 35–39°C, and the enzymatic hydrolysis time is 2–4 h; the enzyme inactivation temperature is 80–90°C, and the enzyme inactivation time is 10–15 min.

[0041] This invention also provides a method for preparing an anti-wrinkle and firming multi-component serum, comprising the following steps:

[0042] (1) Preparation of aqueous phase: Water and water-soluble components in the moisturizing system are mixed and heated to dissolve, thus obtaining an aqueous phase;

[0043] (2) Preparation of oil phase: The oil-soluble components in the anti-wrinkle and firming composition and 1,2-hexanediol in the moisturizing system are mixed and heated to melt to obtain the oil phase;

[0044] (3) Preparation of active phase: Dissolve embryo extract and butanediol solution, add bifida ferment lysate, panthenol, palmitoyl tripeptide-1, soothing ingredients and antioxidant ingredients, stir until uniform to obtain active phase;

[0045] (4) Emulsification: The oil phase and the water phase are mixed, homogenized, and cooled once to obtain an emulsion system;

[0046] (5) Mixing: Mix the emulsion system, active phase, thickener, pH adjuster, preservative, chelating agent and fragrance evenly, and then discharge after a second cooling to obtain the multi-component essence.

[0047] Preferably, the heating temperature in step (1) is 80-85°C;

[0048] The heating temperature in step (2) is 75-80℃;

[0049] In step (3), the mass concentration of the butanediol solution is 8-12%, and the mass ratio of the embryo extract to the butanediol solution is 1:8-12.

[0050] Preferably, in step (4), the homogenization speed is 3000-5000 r / min, the homogenization time is 5-8 min, and the temperature of the first cooling is 45-50℃;

[0051] The temperature for the second cooling in step (5) is 30-35℃.

[0052] This invention employs a stepwise preparation process. In the high-temperature stage (75-85°C), only the heat-resistant components in the aqueous and oil phases are processed. At temperatures below 45°C, active ingredients such as embryo extracts are added to avoid peptide denaturation caused by high temperatures.

[0053] This invention also provides an application of an anti-wrinkle and firming multi-component essence in the preparation of cosmetics or skin care products.

[0054] The present invention has the following beneficial effects:

[0055] 1. Powerful anti-wrinkle and firming: With embryo extract as the core, combined with multiple anti-wrinkle ingredients, it achieves a triple anti-wrinkle mechanism of "promoting synthesis - inhibiting degradation - repairing the barrier". At a concentration of 10%, the elastase inhibition rate is ≥85.5%, which is significantly better than traditional single-ingredient products.

[0056] 2. Multi-effect synergy: In addition to anti-wrinkle and firming, it also has soothing, moisturizing and antioxidant effects, meeting multiple skin care needs in the anti-wrinkle process;

[0057] 3. Gentle and safe: Free of alcohol and strong acidic ingredients, and verified by zebrafish testing and skin irritation testing, suitable for sensitive skin;

[0058] 4. High stability: After storage at 40℃ for 3 months, the active ingredient retention rate is ≥90%, with no layering or discoloration, and the pH remains stable at 5.0~6.0;

[0059] 5. Feasible process: Using conventional cosmetic production equipment, the step-by-step preparation process is simple and controllable, suitable for large-scale production. Attached Figure Description

[0060] Figure 1 This is a comparison chart of the elastase inhibition rates of the multi-component serums in Examples 1 and 2 and the serum in Comparative Example 1.

[0061] Figure 2 This is a graph showing the change in skin elasticity parameter R2 after 8 weeks of use of the multi-component serum in Example 1.

[0062] Figure 3 A comparison of neutrophil counts in zebrafish using the multi-effect face cream from Example 1 and those not using it.

[0063] Figure 4 The image shows the zebrafish irritation test results of the multi-component essence in Example 1.

[0064] Figure 5 The image shows the antioxidant effect of the multi-component essence in Example 1. Detailed Implementation

[0065] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0066] Example 1:

[0067] The raw material formula is as follows (by weight percentage): embryo extract 0.285%, anti-wrinkle and firming composition 0.8% (of which the mass ratio of Bifida ferment lysate, panthenol, squalane, jojoba seed oil, and palmitoyl tripeptide-1 is 0.15:1:0.5:0.5:0.0005), moisturizing system 12.36% (of which the mass ratio of glycerin, propylene glycol, 1,3-propanediol, 1,2-hexanediol, sodium hyaluronate, and acetylated sodium hyaluronate is 8:3:0.78:0.36:0.02:0.023), and soothing ingredients 0.2% (of which bisabolol and chamomile extract are present). The composition includes: 0.195% sodium polyglutamate and 0.0025% rosemary leaf extract (mass ratio: 0.0025: 0.0025); 0.05% antioxidant (with a mass ratio of 0.03:0.02 for sodium polyglutamate and tocopheryl acetate); 0.3% acrylate cross-linked polymer; 0.05% disodium EDTA; 0.405% pH adjuster (with a mass ratio of 0.36:0.045 for arginine and citric acid); 0.0615% preservative (with a mass ratio of 0.06:0.0015 for capryloyl hydroxamic acid and phenoxyethanol); 0.08% fragrance; and the balance being water to bring the total to 100%.

[0068] The multi-effect face cream in this embodiment is prepared using the following method:

[0069] (1) Preparation of aqueous phase: Water, glycerol, propylene glycol, 1,3-propanediol, sodium hyaluronate and acetylated sodium hyaluronate are mixed in proportion and heated at 80°C and stirred at a rate of 300 r / min until completely dissolved to obtain aqueous phase;

[0070] (2) Preparation of oil phase: Squalane, jojoba seed oil and 1,2-hexanediol were mixed in proportion and heated at 75°C and stirred at a rate of 200 r / min until melted to obtain the oil phase;

[0071] (3) Preparation of active phase: The embryo extract and 10% butanediol solution were stirred at 40°C until dissolved (the mass ratio of embryo extract to butanediol was 0.1:1). Then, Bifida ferment lysate, panthenol, palmitoyl tripeptide-1, bisabolol, chamomile extract, rosemary leaf extract, sodium polyglutamate, and tocopheryl acetate were added. The mixture was stirred at 300 r / min for 5 min until homogeneous to obtain the active phase.

[0072] (4) Emulsification: The oil phase is added to the aqueous phase at a rate of 5 mL / min, homogenized at a speed of 4000 r / min for 6 min, and cooled to 45℃ once to obtain the emulsified system.

[0073] (5) Mixing: Add the active phase to the emulsion system and stir at 300r / min for 5min; add acrylate cross-linking polymer and disodium EDTA and stir for 5min; finally add arginine, citric acid, preservative and fragrance and stir for 5min until uniform, cool down to 30℃ for the second time and discharge to obtain a multi-component essence.

[0074] The embryo extract used in this embodiment was prepared using the following method:

[0075] Sheep embryo tissue was collected, freeze-dried at -80℃, pulverized, and mixed with water at a solid-liquid ratio of 1:10. The pH was adjusted to 6.8 with phosphate buffer. Then, a complex protease (with a mass ratio of trypsin and papain of 1:1) at 2% of the embryo tissue mass was added. The mixture was enzymatically hydrolyzed at 37℃ for 3 hours, and then the enzyme activity was inactivated in a water bath at 85℃ for 10 minutes. The supernatant was collected by centrifugation at 5000 r / min for 15 minutes, filtered through a 3000 Da ultrafiltration membrane, and freeze-dried at -55℃ for 12 hours to obtain the embryo extract.

[0076] Example 2

[0077] The difference from Example 1 is that the raw material formulation (by mass percentage) is as follows: 0.5% embryo extract, 1.5% anti-wrinkle and firming composition (of which the mass ratio of Bifida ferment lysate, panthenol, squalane, jojoba seed oil, and palmitoyl tripeptide-1 is 0.2:0.8:0.6:0.6:0.001), 0.1% antioxidant (of which the mass ratio of sodium polyglutamate and tocopheryl acetate is 0.06:0.04), and other active ingredients are the same as in Example 1. The remainder is made up to 100% with water.

[0078] The preparation method is the same as in Example 1.

[0079] Example 3

[0080] The difference from Example 1 is as follows: The raw material formulation (by mass percentage) is: 0.8% anti-wrinkle and firming composition (in which the mass ratio of Bifida ferment lysate, panthenol, squalane, jojoba seed oil, and palmitoyl tripeptide-1 is 0.15:1.2:0.1:0.5:0.0005), 0.0505% preservative (in which the mass ratio of capryloyl hydroxamic acid and phenoxyethanol is 0.05:0.0005), fragrance is removed, 0.01% Centella asiatica extract is added to enhance the soothing effect, other active ingredients are the same as in Example 1, and the remainder is made up to 100% with water;

[0081] The preparation method is the same as in Example 1.

[0082] Comparative Example 1

[0083] The raw material formula is (by mass percentage): embryo extract 0.285%, the remainder is water.

[0084] Effect verification:

[0085] Anti-wrinkle and firming effect

[0086] The multi-component serums from Examples 1, 2, and Comparative Example 1 were diluted to a mass concentration of 10%, and their anti-wrinkle and firming effects were determined by an elastase inhibition rate assay. The experiments used reagents such as TESCA buffer solution, elastase, and MAAPVN solution, as well as equipment including an analytical balance, microplate reader, and constant temperature reactor. The samples were first diluted with TESCA buffer solution as required, and then four reaction systems were set up: well 1 was the experimental group (including the sample group and positive control group), well 2 was the experimental control blank group, well 3 was the negative control group, and well 4 was the negative control blank group. Sample / positive control, TESCA buffer solution, and MAAPVN solution were added to each group according to the specified amounts, and the reaction was carried out at 25°C for 15 minutes; then elastase solution or TESCA buffer solution was added, and the reaction was continued at room temperature (25°C) for another 15 minutes. After the reaction, each reaction solution was transferred to the corresponding 96-well plate, with three replicates per group, and the OD value was read at 410 nm using a microplate reader. The elastase inhibition rate was calculated using the formula: Inhibition rate (%) = [1 - (AB) / (CD)] × 100% (where A is the OD value of pore 1, B is the OD value of pore 2, C is the OD value of pore 3, and D is the OD value of pore 4). The elastase inhibition rate results for each case are as follows: Figure 1 As shown.

[0087] from Figure 1 As can be seen, the elastase inhibition rate of Example 1 was 85.5% ± 0.4% (P < 0.05), while the elastase inhibition rate of Example 2 (high embryo extract) reached 88.3% ± 0.3%, significantly higher than that of Comparative Example 1 (72.1% of the simple embryo extract group). This demonstrates that the multi-component serum in this application exhibits significant differences, indicating its excellent anti-wrinkle and firming effects.

[0088] Skin elasticity effect

[0089] The test subjects were 20 female volunteers aged 30-50 with fine lines on their faces; the test subjects used the multi-component serum of Example 1 twice daily for 8 consecutive weeks. The MPA580 measures skin elasticity parameters. A standardized negative pressure is applied to the skin through a probe, drawing the skin into the probe. The negative pressure is then released, and the instrument records the skin's retraction characteristics and calculates the R² value. During testing, stable ambient temperature and humidity are required. Volunteers should sit still before testing to avoid external skin irritation. Repeated measurements on the same area are necessary to ensure data accuracy and comparability. The changes in the skin elasticity parameter R² are shown below. Figure 2As shown.

[0090] from Figure 2 As can be seen, the R2 value increased from the initial 0.56±0.03 to 0.65±0.02, an increase of 16.1%, indicating that the multi-component essence provided by this invention can significantly improve skin firmness.

[0091] Mildness test

[0092] The assay used transgenic zebrafish (MPX) with green fluorescent neutrophil count, 2 days post-fertilization, with 15 zebrafish per group. During the experiment, zebrafish were randomly selected and placed in 6-well plates. The multi-component essence from Example 1 was dissolved in water to a concentration of 0.25% and administered to the test samples, with a normal control group included. Each well contained 3 ml of the solution, and the plates were incubated at 28°C in the dark for 18 hours. Afterward, 10 zebrafish from each group were photographed under a fluorescence microscope, and the number of skin neutrophils (N) was analyzed using advanced image processing software. Figure 3 As shown, the irritation of the multi-component serum at a concentration of 0.25% was measured. Figure 4 As shown.

[0093] from Figure 3 and Figure 4 As can be seen, the number of neutrophils in the test sample group was similar to that in the normal control group (p>0.05), indicating no significant difference. The irritation rate at the 0.25% concentration was 2.8%. Therefore, it can be concluded that under the conditions of this experiment, this multi-component essence is mild, non-irritating, and has good safety.

[0094] Stability test

[0095] The multi-component essence of Example 1 was placed in environments of 40℃, -10℃ and centrifuged at 3000r / min for 30min respectively, and observed for 3 months. The multi-component essence showed no stratification, discoloration or odor, the pH was stable at 5.5±0.2, and the active ingredient retention rate was 92.6%, which means it has good stability and can be stored for a long time.

[0096] Antioxidant effect

[0097] The antioxidant efficacy of the serum was determined by a free radical scavenging (DPPH) assay, according to method T / SHRH 006-2018. The assay used reagents such as 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) and vitamin E, as well as equipment including pipettes and an ELISA reader. The samples were pretreated by diluting the serum sample from Example 1 with water to multiple concentrations. The pretreated samples were then reacted with DPPH reagent, and the OD value was measured at 517 nm after the reaction. The results were calculated using the formula: "Free radical scavenging rate (%) = [1 - (T – T0) / (C - C0)] × 100%". Where T is the absorbance of the sample tube, T0 is the background absorbance of the sample, C is the average of three DPPH tube absorbance measurements, and C0 is the background absorbance of the solvent. The results are shown below. Figure 5 As shown.

[0098] from Figure 5 As can be seen from the results, the free radical scavenging rate of the 20% concentration sample in Example 1 was 34.25%, indicating that it has antioxidant effects.

[0099] As can be seen from the above embodiments, the present invention provides an anti-wrinkle and firming multi-component essence and its preparation method, which comprises the following components by mass percentage: 0.285-0.5% embryo extract, 0.8-1.5% anti-wrinkle and firming composition, 10-15% moisturizing system, 0.15-0.3% soothing ingredient, 0.03-0.1% antioxidant ingredient, 0.2-0.5% thickener, 0.03-0.08% chelating agent, 0.3-0.6% pH adjuster, 0.05-0.1% preservative, 0.05-0.1% fragrance, and the balance being water. This invention's multi-component essence uses embryonic extract as its core, synergistically combining multiple anti-wrinkle ingredients to achieve a triple anti-wrinkle mechanism of "promoting synthesis, inhibiting degradation, and repairing the skin barrier." At a 10% concentration, it exhibits an elastase inhibition rate of ≥85.5%, significantly superior to traditional single-component products. In addition to anti-wrinkle and firming effects, it also provides soothing, moisturizing, and antioxidant benefits, meeting multiple skincare needs during the anti-wrinkle process. It is alcohol-free and free of strong acidic ingredients, and has been verified through zebrafish testing and skin irritation testing, making it suitable for sensitive skin. After storage at 40℃ for 3 months, the active ingredient retention rate is ≥90%, with no layering or discoloration, and the pH remains stable at 5.0–6.0. ​​Utilizing conventional cosmetic production equipment, the step-by-step preparation process is simple and controllable, suitable for large-scale production.

[0100] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A multi-component anti-wrinkle and firming serum, characterized in that, By weight percentage, it contains the following components: embryo extract 0.285–0.5%, anti-wrinkle and firming composition 0.8–1.5%, moisturizing system 10–15%, soothing ingredients 0.15–0.3%, antioxidant ingredients 0.03–0.1%, thickener 0.2–0.5%, chelating agent 0.03–0.08%, pH adjuster 0.3–0.6%, preservative 0.05–0.1%, fragrance 0.05–0.1%, and the balance being water.

2. The multi-component essence according to claim 1, characterized in that, The embryo extract is derived from sheep embryo tissue, in which small molecule active peptides account for ≥30% and the protein molecular weight distribution is 5000~50000Da.

3. The multi-component essence according to claim 1, characterized in that, The anti-wrinkle and firming composition comprises Bifida ferment lysate, panthenol, squalane, jojoba seed oil, and palmitoyl tripeptide-1, wherein the mass ratio of Bifida ferment lysate, panthenol, squalane, jojoba seed oil, and palmitoyl tripeptide-1 is 0.1–0.2:0.8–1.2:0.1–0.6:0.4–0.6:0.0005–0.001; The moisturizing system comprises glycerin, propylene glycol, 1,3-propanediol, 1,2-hexanediol, sodium hyaluronate, and acetylated sodium hyaluronate, wherein the mass ratio of glycerin, propylene glycol, 1,3-propanediol, 1,2-hexanediol, sodium hyaluronate, and acetylated sodium hyaluronate is 5–8:2–4:0.5–1:0.2–0.5:0.01–0.03:0.02–0.

03. The soothing ingredients include bisabolol, chamomile extract, and rosemary leaf extract, wherein the mass ratio of bisabolol, chamomile extract, and rosemary extract is 0.15–0.25:0.002–0.003:0.002–0.003; The antioxidant components include sodium polyglutamate and tocopheryl acetate, wherein the mass ratio of sodium polyglutamate to tocopheryl acetate is 0.02-0.06:0.01-0.

04.

4. The multi-component essence according to claim 1, characterized in that, The thickener is an acrylic (ester) or a C10-C30 alkanol acrylate crosslinked polymer; The chelating agent is disodium EDTA; The pH adjuster includes arginine and citric acid, wherein the mass ratio of arginine to citric acid is 0.3–0.5:0.03–0.

06. The preservatives include capryloyl hydroxamic acid and phenoxyethanol, wherein the mass ratio of capryloyl hydroxamic acid to phenoxyethanol is 0.05–0.07:0.0005–0.002; The fragrance is a flavoring.

5. A method for preparing an embryo extract, used in the multi-component essence according to any one of claims 1 to 4, characterized in that, Includes the following steps: S1. Sheep embryonic tissue was freeze-dried, pulverized, and mixed with water. The pH was adjusted with phosphate buffer. S2. After adding the complex protease for enzymatic hydrolysis, the enzyme activity is inactivated by water bath. S3. Centrifuge and filter the supernatant, then freeze-dry the filtrate to obtain the embryo extract.

6. The preparation method according to claim 5, characterized in that, In step S1, the solid-liquid ratio of sheep embryonic tissue to water is 1:8-12, and the pH is adjusted to 6.5-7.

0. In step S2, the mass of the complex protease is 1-3% of the mass of the sheep embryonic tissue. The complex protease includes trypsin and papain, wherein the mass ratio of trypsin and papain is 1:

1. In step S2, the enzymatic hydrolysis temperature is 35–39°C, and the enzymatic hydrolysis time is 2–4 h; the enzyme inactivation temperature is 80–90°C, and the enzyme inactivation time is 10–15 min.

7. A method for preparing a multi-component essence as described in any one of claims 1 to 4, characterized in that, Includes the following steps: (1) Preparation of aqueous phase: Water and water-soluble components in the moisturizing system are mixed and heated to dissolve, thus obtaining an aqueous phase; (2) Preparation of oil phase: The oil-soluble components in the anti-wrinkle and firming composition and 1,2-hexanediol in the moisturizing system are mixed and heated to melt to obtain the oil phase; (3) Preparation of active phase: Dissolve embryo extract and butylene glycol solution, add bifida ferment lysate, panthenol, palmitoyl tripeptide-1, soothing ingredients and antioxidant ingredients, stir until uniform to obtain active phase; (4) Emulsification: The oil phase and the water phase are mixed, homogenized, and cooled once to obtain an emulsion system; (5) Mixing: Mix the emulsion system, active phase, thickener, pH adjuster, preservative, chelating agent and fragrance evenly, and then discharge after a second cooling to obtain the multi-component essence.

8. A preparation method according to claim 7, characterized in that, The heating temperature in step (1) is 80-85℃; The heating temperature in step (2) is 75-80℃; In step (3), the mass concentration of the butanediol solution is 8-12%, and the mass ratio of the embryo extract to the butanediol solution is 1:8-12.

9. A preparation method according to claim 7, characterized in that, In step (4), the homogenization speed is 3000-5000 r / min, the homogenization time is 5-8 min, and the temperature of the first cooling is 45-50℃. The temperature for the second cooling in step (5) is 30-35℃.

10. The use of a multi-component essence according to any one of claims 1 to 4 in the preparation of cosmetics or skin care products.

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