A birch juice-black ginseng saponin compound oral liquid, a preparation method and application thereof

Abstract

The present application provides a birch juice-black ginseng saponin compound oral liquid, a preparation method and an application, relates to the field of functional health foods, and comprises the following steps: S1, preparing a birch juice-black ginseng saponin compound: adding black ginseng extract into concentrated birch juice to dissolve, ultrasonicating and oscillating to obtain a birch juice-black ginseng saponin compound; S2, adding a sweetening agent, a stabilizing agent and a preservative, fully dissolving and uniformly stirring; S3, adjusting the pH value, sterilizing, filling and sealing, and packaging to obtain an oral liquid product. The birch juice-black ginseng saponin compound oral liquid, the preparation method and the application are more suitable for long-term use, and are helpful to enhancing the immune function of the body.

Description

Technical Field

[0001] This invention relates to the field of functional health foods, and in particular to a birch sap-black ginsenoside compound oral liquid, its preparation method, and its application. Background Technology

[0002] Black ginseng is a specially processed ginseng product with unique effects and uses. Currently, black ginseng and health foods made from it are becoming increasingly popular. Black ginseng contains rare saponins, polysaccharides, and other active ingredients. These components can stimulate the human immune system, enhance the activity of immune cells, and improve the body's resistance to pathogens, thereby reducing the occurrence of diseases.

[0003] Birch sap is rich in various amino acids, vitamins (such as vitamin C and B vitamins), and minerals (such as calcium, magnesium, and potassium), providing the human body with abundant nutrients. The various active ingredients in birch sap, such as saponins and polysaccharides, help enhance the body's immune function.

[0004] A weakened immune system reduces the body's resistance to pathogens, making it more susceptible to various diseases such as colds, flu, and pneumonia. Furthermore, a weakened immune system can contribute to the development and progression of chronic diseases, such as cancer and autoimmune diseases. The immune system plays a crucial role in maintaining homeostasis and clearing abnormal cells; a decline in immune function impairs these functions, thus affecting overall health. Current methods for boosting immunity include vaccination and medication. Vaccination stimulates the immune system to produce a specific immune response, enhancing resistance to specific pathogens. However, vaccination can also have side effects, such as local redness and swelling, and fever, and is less effective in certain populations (such as those with immunodeficiency). Some medications, such as immunoglobulins and cytokines, can enhance immune function, but these are usually expensive and may have serious side effects, such as allergic reactions and immune dysregulation. Summary of the Invention

[0005] The purpose of this invention is to provide a birch sap-black ginsenoside compound oral liquid, its preparation method and application, which is more suitable for long-term use and helps to enhance the body's immune function.

[0006] To achieve the above objectives, the present invention provides a method for preparing a birch sap-black ginseng saponin compound oral liquid, comprising the following steps: S1. Preparation of birch sap-black ginseng saponin complex: Black ginseng extract is added to concentrated birch sap for dissolution, and after sonication and shaking, birch sap-black ginseng saponin complex is obtained. S2. Add sweetener, stabilizer, and preservative, dissolve thoroughly and stir well; S3: Adjust the pH value to 5.0-6.5, sterilize, fill, and package to obtain the finished oral liquid product.

[0007] Preferably, in S1, the volume ratio of black ginseng extract to concentrated birch sap is 1:(30~50), wherein the birch sap is 6-7 times concentrated birch sap.

[0008] Preferably, in S1, the conditions for one ultrasound session are: 30℃-40℃ and 210~270W power ultrasound for 30-40 minutes.

[0009] Preferably, in S1, the oscillation conditions are: 30℃-40℃, rotation speed of 200-300 r / min, and time of 1-2 h.

[0010] Preferably, in S2, the sweetener is steviol glycoside, the stabilizer is sodium carboxymethyl cellulose, and the preservative is potassium sorbate.

[0011] Preferably, in S3, the pH adjuster includes an alkaline adjuster and an acidic adjuster, wherein the alkaline adjuster is an aqueous solution of sodium bicarbonate with a mass concentration of 8%-10%, and the acidic adjuster is an aqueous solution of citric acid with a mass concentration of 8%-10%.

[0012] Preferably, based on the total liquid weight of 1000 parts of oral liquid, the amount of each main ingredient and excipient added is as follows: Main ingredient components: 540-660 parts by weight of birch sap, 3.2-7.5 parts by weight of black ginseng extract; Excipient components: 0.04-0.08 parts by weight of stevioside, 0.10-0.14 parts by weight of sodium carboxymethyl cellulose, and 0.15-0.20 parts by weight of potassium sorbate.

[0013] A method for preparing a birch sap-black ginsenoside compound oral liquid.

[0014] Application of a birch sap-black ginsenoside compound oral liquid in enhancing the body's immune function.

[0015] Therefore, the present invention employs the above-mentioned birch sap-black ginseng saponin compound oral liquid, its preparation method, and its application, with the following technical effects: By concentrating birch sap and compounding it with black ginseng, and then subjecting it to ultrasound, vibration, and vortexing, a birch sap-black ginseng saponin compound is obtained. A specific proportion of food additives is then added to leverage the synergistic effect of the natural nutrients in birch sap and the bioactive components of black ginseng saponins, resulting in an oral liquid that combines excellent sensory quality with enhanced immune function.

[0016] This invention, through macrophage experiments, discovered that the main ingredient, birch sap-black ginseng saponin complex, can effectively enhance macrophage activity, increase macrophage phagocytic capacity, promote macrophage NO secretion, and induce the release of macrophage cytokines such as TNF-α, IL-6, and IL-1β. The results indicate that the birch sap-black ginseng complex has a significant immune-enhancing function. Attached Figure Description

[0017] Figure 1 The effect of birch sap-black ginseng saponin complex on the activity of RAW264.7 macrophage cells; Figure 2 The effect of birch sap-black ginseng saponin complex on the phagocytic capacity of RAW264.7 macrophages for neutral red; Figure 3 The effect of birch sap-black ginseng saponin complex on RAW264.7 NO content in macrophages; Figure 4 The effect of birch sap-black ginseng saponin complex on cytokine secretion by RAW264.7 macrophages; Figure 4 (a) Effect of different concentrations of birch sap black ginseng compound on the IL-6 cytokine content of RAW264.7 cells; Figure 4 (b) Effects of different concentrations of birch sap and black ginseng compound on the TNF-α cytokine content of RAW264.7 cells; Figure 4 (c) Effects of different concentrations of birch sap and black ginseng compound on the IL-1β cytokine content of RAW264.7 cells. Detailed Implementation

[0018] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0019] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0020] Example 1: The preparation method of birch sap-black ginseng saponin compound oral liquid is as follows: S1: Preparation of birch sap-black ginseng saponin complex. According to the volume ratio, 1 part black ginseng extract was added to 40 parts concentrated birch sap for dissolution. The mixture was sonicated at 240W for 36 minutes and shaken for 1 hour to obtain the birch sap-black ginseng saponin complex. S2: According to the mass ratio, add 0.06 parts of stevioside, 0.12 parts of sodium carboxymethyl cellulose and 0.18 parts of potassium sorbate to the birch sap-black ginsenoside compound prepared in step S1, adjust the pH value to 5.2; sterilize, fill and package to obtain the oral liquid product.

[0021] Example 2: The difference between this embodiment and Embodiment 1 is that 1 part black ginseng extract and 30 parts concentrated birch sap, wherein the birch sap is 6 times concentrated birch sap; the other parts are the same as in Embodiment 1.

[0022] Example 3: The difference between this embodiment and Embodiment 1 is that 1 part black ginseng extract and 50 parts concentrated birch sap, wherein the birch sap is 6 times concentrated birch sap; the other parts are the same as in Embodiment 1.

[0023] Effect Experiment: MTT assay for macrophage survival RAW264.7 cells were placed in DMEM (modified Iago) medium containing 10% FBS (fetal bovine serum), 100 U / ml penicillin, and 100 µg / ml streptomycin, and then cultured in a cell culture incubator maintained at a constant temperature of 37°C and a CO2 concentration of 5%. Cells were passaged when they reached 70-80% confluence. The MTT assay was used to evaluate the effect of different concentrations of birch sap-black ginseng compound treatment on cell viability.

[0024] This invention uses the MTT assay to evaluate the effects of different concentrations of birch sap and black ginseng compound treatment on cell viability. Figure 1 As shown, compared with the untreated blank control group, no toxic effect was observed on cells when the concentration of birch sap black ginseng compound was in the range of 5-30 μg / mL, and the cell survival rate remained above 90% or higher. The cell survival rate was highest when the concentration reached 10 μg / mL, and then decreased due to excessive cell proliferation at 20 μg / mL, showing a trend of first increasing and then decreasing.

[0025] Neutral red staining method for detecting macrophage phagocytic capacity: This invention employs a neutral red phagocytosis assay to detect cellular phagocytic activity. RAW264.7 macrophages were cultured in 96-well plates and treated for 24 hours with different ratios of birch sap-black ginseng compound and LPS (lipopolysaccharide), with six parallel wells per group. The experimental procedure was performed according to the instructions of the neutral red assay kit. Finally, the absorbance was measured at 540 nm using a microplate reader, and the phagocytic rate was calculated.

[0026] like Figure 2Macrophage phagocytic activity is a key indicator of innate immunity and is crucial for maintaining immune defense and homeostasis. Experimental data showed that the LPS-positive control group significantly enhanced macrophage phagocytic function compared to the blank control group. After treatment with 5-30 μg / mL birch sap and black ginseng compound for 24 h, the phagocytic activity of RAW264.7 macrophages was enhanced. The phagocytic rate was highest in the 10 μg / mL treatment group. These results indicate that the birch sap and black ginseng compound has a significant immune-enhancing function and can resist and phagocytose exogenous pathogens.

[0027] Griess method for detecting NO content in macrophages: RAW264.7 cells were cultured in 96-well plates for 24 h, and then treated with different ratios of birch sap-black ginseng compound and LPS (lipopolysaccharide) for 24 h each; each group had 6 parallel wells. NO production was measured using a kit.

[0028] NO is an important cytokine with diverse biological activities, widely involved in immune responses. NO acts on the immune response system, inducing the expression of related factors such as IL-6 and TNF-α, thereby participating in immune regulation. Therefore, increased NO release levels are an important indicator of immune activation. Figure 3 As shown, this invention used the Griess reagent colorimetric method to detect the NO production level of RAW264.7 cells stimulated by a 5-30 μg / mL concentration gradient of birch sap-black ginseng saponin complex. The results showed that, compared with the untreated blank control group, the LPS-positive control group significantly promoted NO secretion (P<0.01), and the NO level in the treated groups first increased and then decreased with increasing drug concentration. This indicates that the birch sap-black ginseng saponin complex promotes NO secretion in RAW264.7 cells by stimulating their activation, thus confirming its potential immunomodulatory activity.

[0029] Detection of macrophage cytokine levels using the ELISA kit: RAW264.7 cells were placed in 96-well plates and treated with different ratios of birch sap-black ginseng compound and LPS (lipopolysaccharide) for 24 h; each group had 6 parallel wells. Cell culture supernatant was collected by centrifugation, and the levels of IL-1β, IL-6, and TNF-α were detected using an ELISA kit according to the manufacturer's instructions. The absorbance at 450 nm was recorded using a microplate reader.

[0030] The results are as follows Figure 4 As shown in the figure, compared with the blank control group, both the LPS positive control group and the treatment groups with different concentrations of birch sap-black ginseng saponin complex upregulated the level of IL-6 cytokine. This result indicates that the birch sap-black ginseng saponin complex can effectively stimulate the activation of RAW264.7 macrophages, induce the release of various immunomodulatory factors, and thus enhance their immunomodulatory activity.

[0031] Determination of physicochemical properties of birch sap-black ginsenoside compound oral liquid; (1) Determination of pH value of oral solution Table 1 pH values ​​of oral solutions

[0032] Testing revealed that the pH value of the oral liquid remained stable at around 5.20, which meets the pH requirements for oral liquids in the Chinese Pharmacopoeia.

[0033] (2) Stability test of oral solution Table 2 Effect of Temperature on Oral Liquids

[0034] The effect of temperature on the appearance of the oral liquid is shown in Table 2. At different temperatures, the oral liquid consistently appeared brownish-red, with minimal variation in saponin content. It remained clear throughout, with a pH range of 5.18–5.21 and a pH value between 12 and 12.4. Microbial limits met the standards. Therefore, the effect of temperature on the quality of the oral liquid is negligible.

[0035] Table 3 Effects of light on oral liquids

[0036] The effect of light exposure on the appearance of the oral liquid is shown in Table 3. Under different light conditions, the saponin content of the oral liquid did not change significantly in the first 10 days, remaining stable between 12.2 and 12.4. However, compared to oral liquid products stored away from light, the saponin content decreased on the 20th day under direct sunlight. Therefore, to better preserve the content of the active ingredient saponin in the oral liquid, it should be stored in a brown bottle away from light.

[0037] (3) Determination of relative density of oral liquid Table 4 Relative Density of Oral Liquid

[0038] Three batches of the oral liquid were collected. Considering differences in production processes and stability, and according to the Chinese Pharmacopoeia, the relative density of the finished product must not be lower than 1.05. The relative density test results of this oral liquid meet the requirements.

[0039] (4) Determination of saponin content in oral liquid Table 5. Saponin content in oral liquid

[0040] According to the standard requirements for ginseng oral liquid health food, the total saponin content should be ≥10mg / 100mL. As shown in Table 5, the saponin content of the product meets the requirements.

[0041] Therefore, the birch sap-black ginsenoside compound oral liquid, preparation method and application of the present invention are more suitable for long-term use and help to enhance the body's immune function.

[0042] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A method for preparing a birch sap-black ginseng saponin compound oral liquid, characterized in that, Includes the following steps: S1. Preparation of birch sap-black ginseng saponin complex: Black ginseng extract is dissolved in concentrated birch sap, and after sonication and shaking, birch sap-black ginseng saponin complex is obtained. S2. Add sweetener, stabilizer, and preservative, dissolve thoroughly and stir well; S3: Adjust the pH value to 5.0-6.5, sterilize, fill, and package to obtain the finished oral liquid product.

2. The method for preparing a birch sap-black ginseng saponin compound oral liquid according to claim 1, characterized in that, In S1, the volume ratio of black ginseng extract to concentrated birch sap is 1:(30~50), wherein the birch sap is 6-7 times more concentrated.

3. The method for preparing a birch sap-black ginseng saponin compound oral liquid according to claim 1, characterized in that, In S1, the conditions for one ultrasound session are: 30℃-40℃, using 210~270W power for 30-40 minutes of ultrasound.

4. The preparation method of the birch sap-black ginseng saponin compound oral liquid according to claim 1, characterized in that, In S1, the oscillation conditions are: 30℃-40℃, rotation speed of 200-300 r / min, and time of 1-2 h.

5. The preparation method of the birch sap-black ginsenoside compound oral liquid according to claim 1, characterized in that, In S2, the sweetener is stevioside, the stabilizer is sodium carboxymethyl cellulose, and the preservative is potassium sorbate.

6. The method for preparing a birch sap-black ginseng saponin compound oral liquid according to claim 1, characterized in that, In S3, the pH adjuster includes an alkaline adjuster and an acidic adjuster. The alkaline adjuster is an aqueous solution of sodium bicarbonate with a mass concentration of 8%-10%, and the acidic adjuster is an aqueous solution of citric acid with a mass concentration of 8%-10%.

7. The method for preparing a birch sap-black ginseng saponin compound oral liquid according to claim 5, characterized in that, Based on the total liquid weight of 1000 oral liquids, the amounts of each main ingredient and excipient are as follows: Main ingredients: 540-660 parts by weight of birch sap, 3.2-7.5 parts by weight of black ginseng extract; Excipients: 0.04-0.08 parts by weight of stevioside, 0.10-0.14 parts by weight of sodium carboxymethyl cellulose, and 0.15-0.20 parts by weight of potassium sorbate.

8. A birch sap-black ginsenoside compound oral liquid prepared by the preparation method of the birch sap-black ginsenoside compound oral liquid according to claim 1.

9. The application of the birch sap-black ginsenoside compound oral liquid according to claim 8 in enhancing the body's immune function.

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