Camellia anti-aging compositions for cell priming and regulation of DNA methylation homeostasis

Abstract

The present application relates to a camellia anti-aging composition for charging cells and regulating DNA methylation homeostasis. The camellia anti-aging composition comprises magnolia flower extract, olea leaf extract, camellia flower extract and camellia leaf extract. Through the mechanism research on the epigenetic level of aging, the present application finds that the expression of six methylation-related enzymes is obviously disordered, resulting in defects in energy metabolism of aging cells. Based on this, the present application utilizes the synergistic effect of the four extracts to down-regulate the over-activated DNMT1, DNMT3B, TET2 and TET3 to normal, and up-regulate the inhibited DNMT3A and TET1 to normal, reverse the abnormal expression of the six methylation-related enzymes, and systematically restore the skin DNA methylation homeostasis from the epigenetic level, thereby significantly enhancing the energy metabolism of skin cells, improving the defects in energy metabolism of aging cells, and further realizing the anti-aging effect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a camellia anti-aging composition that energizes cells and regulates DNA methylation homeostasis. Background Technology

[0002] Current research on camellia flower and leaf extracts primarily focuses on increasing the content of flavonoid aglycones such as quercetin through enzymatic hydrolysis or macroporous resin purification, thereby enhancing downstream effects such as antioxidant activity, promotion of type I collagen synthesis, and cell migration and repair. However, these strategies only address the surface signs of aging and fail to address the fundamental driving factor at the epigenetic level, namely DNA methylation imbalance, resulting in limited anti-aging efficacy of current strategies.

[0003] For example, Chinese patent application CN118773273A discloses a method and application for preparing anti-aging raw materials from camellia flowers and leaves using enzymatic hydrolysis. Although it can prepare quercetin to some extent by enzymatically hydrolyzing quercetin flavonoid glycosides in camellia flower extract and camellia leaf extract, the anti-aging efficacy of a single quercetin active ingredient remains limited. It is evident that the existing disclosed technology does not reveal any influence of camellia flower and leaf extracts on the DNA methylation regulatory enzyme system when combined with other plant components, nor does it achieve systematic methylation homeostasis repair.

[0004] Therefore, there is an urgent need to conduct in-depth research into the mechanisms of anti-aging and to develop an anti-aging composition that regulates DNA methylation imbalance by combining camellia flower and leaf extracts with other plant components. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a camellia anti-aging composition that revitalizes cells and regulates DNA methylation homeostasis. Through epigenetic mechanism research on aging, this invention discovered significant dysregulation in the expression of six methylation-related enzymes, leading to defects in energy metabolism in aging cells. Based on this, this invention utilizes the synergistic effect of four extracts to downregulate overactivated DNMT1, DNMT3B, TET2, and TET3 to normal levels, while simultaneously upregulating inhibited DNMT3A and TET1 back to normal, reversing the abnormal expression of the six methylation-related enzymes. This systematically restores skin DNA methylation homeostasis at the epigenetic level, significantly enhancing skin cell energy metabolism, improving energy metabolism defects in aging cells, and thus achieving anti-aging effects. This fills the technological gap in the regulation of skin DNA methylation homeostasis using a combination of natural products.

[0006] The objective of this invention can be achieved through the following methods: In a first aspect, the present invention provides a camellia anti-aging composition for energizing cells and regulating DNA methylation homeostasis, comprising the following components by weight: Magnolia flower extract, 0.003-0.01 parts; Olive leaf extract, 0.001-0.009 parts; Camellia flower extract 0.1-1 part; Camellia leaf extract 0.1-1 part; Add water to make 100 servings.

[0007] As one embodiment of the present invention, the magnolia extract contains magnolin ≥100ppm.

[0008] As one embodiment of the present invention, the method for preparing the magnolia flower extract includes the following steps: A1. Take dried magnolia flowers as raw material, crush them, and sieve them to obtain coarse powder; A2. After soaking the coarse powder in ethanol, add enzyme hydrolysis to obtain a purified enzymatic hydrolysate. A3. Extract the enzyme hydrolysate to obtain an extract; A4. After adsorbing the extract with a macroporous adsorption resin column, elute with ethanol to obtain the eluent. A5. After concentrating the eluent, obtain magnolia flower extract.

[0009] Furthermore, in step A1, the mesh size of the sieve is 30-50 mesh.

[0010] Further, in step A2, the volume concentration of ethanol is 50% to 70%; and the enzymatic hydrolysis time is 1-2 hours.

[0011] Furthermore, in step A3, the extraction is carried out in a constant temperature water bath at a temperature of 60-80°C for a time of 100-150 min.

[0012] Furthermore, in step A5, the concentration is carried out in a concentration tank at a temperature of 40-60°C and a pressure of 0.1-0.3 MPa.

[0013] As one embodiment of the present invention, the main active ingredient of the olive extract includes oleuropein.

[0014] As one embodiment of the present invention, the method for preparing the olive extract includes the following steps: B1. After drying the olive leaves, crush them. B2. Add the extraction reagent to the crushed olive leaves and extract to obtain the extract; B3. Concentrate the extract to obtain olive leaf extract.

[0015] Further, in step B1, the drying temperature is 110-130℃ and the time is 10-15 min; the material is crushed to below 0.5cm.

[0016] Further, in step B2, the extraction is carried out in an extraction vessel at a temperature of 60-80°C for 8-12 hours; the extraction reagent includes a 50-70% ethanol solution by volume, and the volume ratio of olive leaves to the extraction reagent is 1:10-15.

[0017] Furthermore, in step B3, the concentration is carried out in a concentration tank at a temperature of 40-60°C and a pressure of 0.1-0.3 MPa.

[0018] As one embodiment of the present invention, the main active ingredient of the camellia flower extract or camellia leaf extract includes quercetin ≥300ppm.

[0019] As one embodiment of the present invention, the preparation method of the camellia flower extract or camellia leaf extract includes the following steps: C1. Add camellia powder to an ethanol-water solution, then sonicate, stir and extract, filter to obtain camellia extract; C2. The camellia extract is pretreated by adsorption purification through macroporous resin, then β-glucosidase is added, the mixture is stirred and enzymatically hydrolyzed, then the enzyme is inactivated by heating, and the extract is obtained by filtration. The macroporous resin adsorption purification process includes the following steps: passing the camellia extract through a macroporous resin adsorption column, then rinsing the macroporous resin column with water until the effluent is colorless; first eluting with a 28-35% ethanol solution, then eluting with a 40-45% ethanol solution, and the resulting eluent is the pretreated camellia extract.

[0020] Further, in step C1, the camellia powder is camellia flower powder and / or camellia leaf powder; And / or, the ethanol aqueous solution contains 10-90% ethanol by mass; And / or, the amount of ethanol aqueous solution added is 10-30 times the mass of camellia powder; And / or, the ultrasonic power of the ultrasonic treatment is 500W-3000W, and the ultrasonic time is 10min-60min; And / or, the temperature of the ultrasound is below 40°C; And / or, the temperature for stirring extraction is 40-70℃, and the time is 1-4h.

[0021] Furthermore, in step C2, the mass of β-glucosidase is 0.1-2% of the mass of camellia powder; the temperature for stirring and enzymatic hydrolysis is 40-70℃, and the time is 0.5h-4h.

[0022] Secondly, the present invention provides a method for preparing the camellia anti-aging composition, comprising the following steps: Step 1: Weigh out water and magnolia flower extract, stir well to obtain mixture 1; Step 2: Weigh out the olive leaf extract, add it to mixture 1, and stir well to obtain mixture 2; Step 3: Weigh out camellia flower extract and camellia leaf extract, add them to mixture 2, stir well, and obtain the camellia anti-aging composition.

[0023] In one embodiment of the present invention, the stirring speed is 100-300 r / min.

[0024] Thirdly, the present invention also provides the use of the aforementioned camellia anti-aging composition in the preparation of cosmetics.

[0025] Fourthly, the present invention also provides a cosmetic containing 0.001 to 1 wt.% of the camellia anti-aging composition.

[0026] As one embodiment of the present invention, the cosmetic includes one of facial cleanser, facial cleansing cream, makeup remover, makeup remover water, shampoo, and shower gel.

[0027] This invention reveals that UVA stimulation can disrupt cellular DNA methylation homeostasis, leading to significant disruption of methyltransferase expression and demonstrating an epigenetic mechanism of aging. Specifically, the expression of promethyltransferases DNMT1 and DNMT3B and demethyltransferases TET2 and TET3 is significantly upregulated, while the expression of DNMT3A and TET1 is significantly downregulated. Based on this, this invention develops an anti-aging composition at the epigenetic level. Its mechanism of action is as follows: the compound composition, composed of magnolia flower extract, olive leaf extract, camellia flower extract, and camellia leaf extract, firstly exhibits synergistic antioxidant activity, laying the foundation for a redox environment conducive to methylation homeostasis repair. Specifically, olive leaf extract scavenge free radicals at their source, magnolia flower extract inhibits the inflammatory cascade, and quercetin in camellia flower / leaf extract activates the Nrf2 pathway to enhance endogenous antioxidant capacity, collectively protecting DNMT and TET enzymes from oxidative inactivation. Secondly, the compound formulation provides bidirectional and synergistic regulation of methyltransferases, reversing the abnormal expression of six methylation-related enzymes induced by UVA. It downregulates overactivated DNMT1, DNMT3B, TET2, and TET3 to normal levels, while simultaneously upregulating inhibited DNMT3A and TET1 back to normal, systematically restoring skin DNA methylation homeostasis at the epigenetic level. Specifically, the core active biphenyl lignans in magnolium flower extract, magnolol and honokiol, target and downregulate abnormally overexpressed DNMT1, TET2, and TET3, inhibiting oxidative stress-induced enzyme overactivation by binding to the enzyme catalytic pocket, while simultaneously preventing… This treatment disrupts inflammatory pathways, prevents hypomethylation of pro-inflammatory gene promoters, and stabilizes the basic methylation framework. Olive leaf extract, containing oleuropein, competitively inhibits the overexpression of DNMT1, DNMT3B, and TET3, clears ROS to prevent oxidative inactivation of DNMT and TET, and alleviates UVA-induced methyltransferase dysregulation at its source, reducing the abnormally high trend of de novo methylation (PMC). Camellia flower extract, rich in quercetin, bidirectionally regulates methyltransferases: downregulating DNMT1, DNMT3B, TET2, and TET3, and upregulating UV-inhibited DNMT3A and TET1; it also activates the Nrf2 pathway to increase intracellular NAD. +The formula provides cofactors for TET1 demethylation, repairing de novo methylation imbalance. Camellia leaf extract epigallocatechin gallate, in synergy with quercetin, inhibits excessive DNMT1 accumulation, stabilizes normal DNMT3A expression, and helps downregulate TET2 and TET3, maintaining the basic activity of TET1. Finally, the compound composition, based on the bidirectional regulation of the above six DNA methyltransferases, significantly enhances energy metabolism, improves metabolic defects in aging cells, and achieves anti-aging effects. The four extracts form a targeted closed loop, synergistically restoring the six UVA-disordered methyltransferases to physiological homeostasis, rebuilding the skin's DNA methylation balance. This solves the problem that existing anti-aging products only act on downstream events such as oxidative stress and collagen degradation, failing to address the upstream driving factor of epigenetic DNA methylation imbalance, achieving a breakthrough effect of systematically reversing skin aging from the perspective of epigenetic programming.

[0028] Compared with the prior art, the present invention has the following beneficial effects: 1. The compound composition of the present invention utilizes olive leaf extract to scavenge free radicals at the source, magnolia flower extract to inhibit the inflammatory cascade, and quercetin in camellia flower / leaf extract to activate the Nrf2 pathway and enhance endogenous antioxidant capacity. Together, they protect DNMT and TET enzymes from oxidative inactivation, achieving synergistic antioxidant effects and laying the foundation for redox environment for methylation homeostasis repair.

[0029] 2. This invention utilizes a compound composition consisting of magnolia flower extract, olive leaf extract, camellia flower extract, and camellia leaf extract to downregulate overactivated DNMT1, DNMT3B, TET2, and TET3 to normal levels, while simultaneously upregulating inhibited DNMT3A and TET1 back to normal. This reverses the abnormal expression of six methylation-related enzymes induced by UVA, systematically restoring skin DNA methylation homeostasis at the epigenetic level, thereby significantly enhancing skin cell energy metabolism and achieving anti-aging effects.

[0030] 3. The compound composition of this invention achieves multiple effects of anti-wrinkle, repair, anti-oxidation and anti-inflammation, filling the technical gap of natural product compound regulation of skin DNA methylation homeostasis. Moreover, the preparation process is simple, the ingredients are natural and safe, and it is suitable for various anti-aging skin care products. Attached Figure Description

[0031] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings: Figure 1 The ROS expression levels are relative to those of Examples 1-3 and Comparative Examples 1-4. Detailed Implementation

[0032] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The following examples are implemented under the premise of the technical solution of the present invention, providing detailed implementation methods and specific operating procedures, which will help those skilled in the art to further understand the present invention. It should be noted that the scope of protection of the present invention is not limited to the following embodiments; any adjustments and improvements made under the concept of the present invention are all within the scope of protection of the present invention.

[0033] The sources and specifications of the raw materials used in the following examples and comparative examples are as follows: Magnolia flower extract: Prepared according to the method described in Chinese patent application CN114712274 A, the obtained extract contains magnolin content ≥100ppm, and the specific preparation method is as follows: 1) Take dried magnolia flowers as raw material, crush them, and pass them through a 30-50 mesh sieve to obtain coarse powder; 2) After soaking the coarse powder in 70% ethanol, add enzyme hydrolysis for 2 hours to obtain the purified enzymatic hydrolysate; 3) Soak the enzymatic hydrolysate in a 70℃ constant temperature water bath for 120 minutes; 4) After adsorption with a macroporous adsorption resin column, elute with ethanol to obtain the eluent; 5) Transfer the above eluent to a concentration tank and concentrate at 0.1 MPa and 50°C to obtain magnolia flower extract.

[0034] Olive leaf extract: Prepared according to the method described in Chinese patent application CN114712274 A, the main active ingredients of the obtained extract are oleuropein and olive polyphenols, and the specific preparation method is as follows: 1) Dry the olive leaves at 120℃ for 12 minutes, then crush them to less than 0.5cm. 2) Place the crushed olive leaves into an extraction vessel, and use 50% ethanol solution as the extraction reagent at a volume ratio of 1:15. Extract at 70℃ for 10 hours. 3) Transfer the extract to a concentration tank and concentrate it at 0.1 MPa and 50°C to obtain olive leaf extract.

[0035] Camellia flower extract and camellia leaf extract: prepared according to Example 5 of CN118773273A, the quercetin content in the obtained extracts is ≥300ppm.

[0036] The preparation methods of the camellia anti-aging compositions in the examples and comparative examples include the following steps: Step 1: Weigh out water and magnolia flower extract, stir thoroughly at a speed of about 200 r / min to obtain mixture 1; Step 2: Weigh out the olive leaf extract and add it to mixture 1. Stir thoroughly at a speed of about 200 r / min to obtain mixture 2. Step 3: Weigh out the camellia flower extract and camellia leaf extract, add them to mixture 2, and stir thoroughly at a stirring speed of about 200 r / min to obtain the camellia anti-aging composition.

[0037] Examples 1-3 The formulations of the camellia anti-aging compositions in Examples 1-3 are shown in Table 1: Table 1

[0038] Comparative Examples 1-4 The formulations of the camellia anti-aging compositions for Comparative Examples 1-4 are shown in Table 2: Table 2

[0039] Efficacy test 1. Antioxidant test and DNA methyltransferase test Using a UVA-induced skin cell oxidative stress and methylation homeostasis disorder model, intracellular ROS levels (antioxidant test) and the relative expression levels of six methylation-related enzymes (DNA methyltransferase test) were detected to evaluate the regulatory effect of the compound composition of the present invention on oxidative stress and DNA methylation homeostasis.

[0040] Antioxidant test: Human skin fibroblasts in logarithmic growth phase were digested and seeded into 96-well cell culture plates (100 µL per well) and incubated at 37 ℃ in a 5% CO2 incubator. After 24 hours of adhesion, the culture medium was discarded, and different concentrations of complete culture medium for the tested samples (DMEM high-glucose medium (containing sodium pyruvate, Gibco, #11995065)) were added. An equal volume of complete culture medium was added to the control group. Each concentration was used in at least three replicates. After 24 hours of incubation, the culture medium was discarded, and CCK-8 working solution was added. Blank wells were also included, and the cells were incubated for 1-2 hours. Cells were collected, washed 2-3 times with PBS, and incubated with DCFH-DA probe dye at 37 ℃ in the dark for 30 minutes. After staining, the cells were washed 2-3 times with PBS, resuspended, and mitochondrial ROS content was detected by flow cytometry.

[0041] Methyltransferase test: Cells in the logarithmic growth phase were seeded at an appropriate density in 6 cm cell culture plates and cultured for 24 h until cell confluence reached 70%-80%. The culture medium was then discarded, cells were washed with PBS, and irradiated with UVA. Subsequently, the treatment group was added to complete culture medium containing the test sample (DMEM high-glucose medium (containing sodium pyruvate, Gibco, #11995065)), while the control group was added to an equal volume of complete culture medium, and culture continued for another 24 h. After culture, the culture medium was discarded, cells were washed twice with PBS, and total RNA was extracted from each group of cells according to the kit instructions. After purity testing, the RNA was reverse transcribed into cDNA. The expression levels of DNMT1, DNMT3A, DNMT3B, TET1, TET2, and TET3 genes were detected by real-time quantitative PCR. Using GAPDH as an internal reference gene, the relative expression levels of each target gene were calculated using the 2^-ΔΔCt method, as shown in the following formula: ΔCt = Ct target gene - Ct internal reference; ΔCt = ΔCt treatment group - ΔCt control group; Relative gene expression level = 2^-ΔΔCt.

[0042] Compared with the normal control group, the UVA model group showed a highly significant increase in intracellular ROS levels (####, P < 0.0001); at the same time, the expression of methyltransferases was significantly disordered: the expression of promethyltransferases DNMT1 and DNMT3B and demethyltransferases TET2 and TET3 was significantly upregulated, while the expression of DNMT3A and TET1 was significantly downregulated, confirming that UVA stimulation can induce oxidative stress imbalance and disrupt cellular DNA methylation homeostasis.

[0043] Combination Figure 1 The ROS detection results shown indicate that the complete compound systems of Examples 1-3 can significantly reduce UVA-induced increases in intracellular ROS. (P < 0.001), and the antioxidant capacity gradually increased with the increase of the ratio of magnolia flower extract, camellia flower extract, and camellia leaf extract. Example 3 showed the best ROS scavenging effect. Olive leaf extract and magnolia flower extract are the core antioxidant components of this system, which can scavenge ROS at the source, prevent DNMT and TET enzymes from being inactivated by oxidative damage, and lay the foundation for the redox environment for methylation homeostasis repair.

[0044] Table 3 shows that the quantitative results of methyltransferases indicate that Examples 1-3 can bidirectionally regulate six methylation-related enzymes, gradually reversing the expression abnormalities caused by UVA. With optimized concentrations of active components, Example 3 showed the best regulatory effect: it downregulated the abnormally overexpressed DNMT1, DNMT3B, TET2, and TET3 to near normal levels, while simultaneously restoring the UVA-inhibited DNMT3A and TET1, thus restoring methyltransferase expression to physiological homeostasis. Further increasing the concentration of active components would lead to increased cytotoxicity and would not be of experimental significance.

[0045] Comparative Examples 1-4 were respectively missing any one of the active components of magnolia flower extract, olive leaf extract, camellia flower extract, and camellia leaf extract. Antioxidant results showed that although the ROS levels in each comparative example were significantly lower than those in the UVA group, their ROS scavenging ability was significantly weaker than that of the complete formulation examples of the same gradient. Methyltransferase detection further confirmed that the absence of a single component would cause disruption of regulatory pathways.

[0046] Among them, Comparative Example 1, which lacked magnolia flower extract, could not effectively inhibit the abnormally high expression of DNMT1, TET2, and TET3; Comparative Example 2, which lacked olive leaf extract, could not effectively downregulate DNMT1, DNMT3B, and TET3; Comparative Example 3, which lacked camellia flower extract, and Comparative Example 4, which lacked camellia leaf extract, showed a significant decrease in their ability to upregulate DNMT3A and TET1, and could not effectively repair the de novo methylation imbalance.

[0047] Table 3

[0048] 2. Energy metabolism test Test method: p-AMPK is a core sensing kinase for cellular energy homeostasis. During cellular aging, the energy sensing pathway becomes deactivated, and insufficient p-AMPK activation directly disrupts a series of downstream physiological regulations. PGC1α is a key transcription factor for mitochondrial synthesis. In aging cells, mitochondria are damaged and regeneration is hindered, resulting in persistently low PGC1α expression and a significant decrease in cellular metabolic supply capacity. Together, these two constitute the cellular metabolic regulatory axis, and metabolic imbalance accelerates skin aging.

[0049] HFF cells in logarithmic growth phase were seeded in 6 cm diameter cell culture dishes and cultured in DMEM high-glucose medium (Gibco, #11995065). After reaching approximately 70% confluence, cells were irradiated with UVA. After irradiation to a certain dose, different concentrations of samples from Examples 1-3 and Comparative Examples 1-4 were added for further culture. The culture medium was replaced with fresh medium, and the cells were cultured for another 24 h. The supernatant was discarded, cells were collected, lysed, and centrifuged. The protein concentration of the supernatant was determined using the BCA method. An equal volume of total protein was subjected to SDS-PAGE electrophoresis and transferred to a PVDF membrane. After blocking, the membrane was incubated with primary antibodies of Phospho-AMPKα (Thr172) (Cell Signaling Technology), PGC-1α (Proteintech), and GAPDH (Beyotime). Subsequently, the cells were incubated with HRP-labeled goat anti-rabbit IgG and HRP-labeled goat anti-mouse IgG (secondary antibody, Beyotime). Finally, ECL chemiluminescence imaging was used, with GAPDH as an internal control, to perform semi-quantitative analysis of the expression levels of p-AMPKα and PGC-1α proteins.

[0050] Table 4

[0051] Table 4 shows that the methyltransferases in the UVA model control group were significantly disordered, with only a slight upregulation of p-AMPK and almost no change in PGC1α, indicating that the cell's own metabolic repair mechanism was blocked. Examples 1-3 were able to bidirectionally correct methyltransferase expression and restore DNA methylation homeostasis; with the increase of the active components in the formulation, the expression of p-AMPK and PGC1α showed a gradient upregulation, with Example 3 showing the best effect on methyltransferase repair and the most significant increase in the expression of the corresponding two proteins.

[0052] Because each comparative example lacked any plant extract, it could not effectively correct the abnormal expression of methyltransferases, resulting in significant defects in methylation homeostasis repair. Consequently, p-AMPK and PGC1α only showed a slight increase, far less than in the example group. This data confirms that this composition regulates methyltransferase levels, restores methylation homeostasis, and synergistically upregulates p-AMPK and PGC1α, thereby improving metabolic defects in senescent cells and exerting an anti-aging effect at the epigenetic level.

[0053] The above results demonstrate a significant synergistic effect among the four extracts: magnolia flower extract, olive leaf extract, camellia flower extract, and camellia leaf extract. The four components, when combined, form a targeted regulatory closed loop. Only when all four extracts coexist can they simultaneously improve oxidative stress imbalance, systematically repair the disordered expression of six methyltransferases induced by UVA, rebuild skin cell DNA methylation homeostasis, regulate cellular energy metabolism, and achieve epigenetic anti-aging. The absence of any one of the extracts leads to a decrease in antioxidant effect, a significant defect in methylation homeostasis repair function, and a significant decrease in energy metabolism levels.

[0054] The specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various modifications or variations within the scope of the claims, which do not affect the essence of the present invention.

Claims

1. A camellia anti-aging composition that energizes cells and regulates DNA methylation homeostasis, characterized in that, By weight, it contains the following components: Magnolia flower extract, 0.003-0.01 parts; Olive leaf extract, 0.001-0.009 parts; Camellia flower extract 0.1-1 part; Camellia leaf extract 0.1-1 part; Add water to make 100 servings.

2. The camellia anti-aging composition according to claim 1, characterized in that, The magnolia flower extract contains ≥100 ppm of magnolin; the preparation method of the magnolia flower extract includes the following steps: A1. Take dried magnolia flowers as raw material, crush them, and sieve them to obtain coarse powder; A2. After soaking the coarse powder in ethanol, add enzyme hydrolysis to obtain a purified enzymatic hydrolysate. A3. Extract the enzyme hydrolysate to obtain an extract; A4. After adsorbing the extract with a macroporous adsorption resin column, elute with ethanol to obtain the eluent. A5. After concentrating the eluent, obtain magnolia flower extract.

3. The camellia anti-aging composition according to claim 2, characterized in that, In step A1, the mesh size of the sieve is 30-50 mesh; And / or, in step A2, the volume concentration of ethanol is 50% to 70%; the enzymatic hydrolysis time is 1-2 hours; And / or, in step A3, the extraction is carried out in a constant temperature water bath at a temperature of 60-80°C for a time of 100-150 min. And / or, in step A5, the concentration is carried out in a concentration tank at a temperature of 40-60°C and a pressure of 0.1-0.3 MPa.

4. The camellia anti-aging composition according to claim 1, characterized in that, The main active ingredient of the olive oil extract includes oleuropein; the preparation method of the olive oil extract includes the following steps: B1. After drying the olive leaves, crush them. B2. Add the extraction reagent to the crushed olive leaves and extract to obtain the extract; B3. Concentrate the extract to obtain olive leaf extract.

5. The camellia anti-aging composition according to claim 4, characterized in that, In step B1, the drying temperature is 110-130℃ and the time is 10-15 min; the material is then crushed to a size below 0.5cm. And / or, in step B2, the extraction is carried out in an extraction vessel at a temperature of 60-80°C for 8-12 hours; the extraction reagent includes a 50-70% ethanol solution, and the volume ratio of olive leaves to the extraction reagent is 1:10-15. And / or, in step B3, the concentration is carried out in a concentration tank at a temperature of 40-60°C and a pressure of 0.1-0.3 MPa.

6. The camellia anti-aging composition according to claim 1, characterized in that, The main active ingredient of the camellia flower extract or camellia leaf extract includes quercetin ≥300ppm; the preparation method of the camellia flower extract or camellia leaf extract includes the following steps: C1. Add camellia powder to an ethanol-water solution, then sonicate, stir and extract, filter to obtain camellia extract; C2. The camellia extract is pretreated by adsorption purification through macroporous resin, then β-glucosidase is added, the mixture is stirred and enzymatically hydrolyzed, then the enzyme is inactivated by heating, and the extract is obtained by filtration. The macroporous resin adsorption purification process includes the following steps: passing the camellia extract through a macroporous resin adsorption column, then rinsing the macroporous resin column with water until the effluent is colorless; first eluting with a 28-35% ethanol solution, then eluting with a 40-45% ethanol solution, and the resulting eluent is the pretreated camellia extract.

7. The camellia anti-aging composition according to claim 6, characterized in that, In step C1, the camellia powder is camellia flower powder and / or camellia leaf powder; The ethanol content in the aqueous solution is 10-90% by mass; The amount of ethanol-water solution added is 10-30 times the mass of the camellia powder; The ultrasonic power for ultrasonic treatment is 500W-3000W, and the ultrasonic time is 10min-60min. The temperature of ultrasound is below 40℃; The temperature for stirring extraction is 40-70℃, and the time is 1-4 hours; And / or, in step C2, the mass of β-glucosidase is 0.1-2% of the mass of camellia powder; the temperature for stirring and enzymatic hydrolysis is 40-70℃, and the time is 0.5h-4h.

8. A method for preparing the camellia anti-aging composition according to any one of claims 1-7, characterized in that, Includes the following steps: Step 1: Weigh out water and magnolia flower extract, stir well to obtain mixture 1; Step 2: Weigh out the olive leaf extract, add it to mixture 1, and stir well to obtain mixture 2; Step 3: Weigh out camellia flower extract and camellia leaf extract, add them to mixture 2, stir well, and obtain the camellia anti-aging composition.

9. Use of the camellia anti-aging composition according to any one of claims 1-7 in the preparation of cosmetics.

10. A cosmetic product, characterized in that, The cosmetic contains 0.001 to 1 wt.% of the camellia anti-aging composition according to any one of claims 1-7, and the cosmetic includes one of facial cleanser, facial cleansing cream, makeup remover, makeup remover water, shampoo, and shower gel.

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