Beauveria bassiana engineering bacteria expressing Hv1a toxin and application thereof in pest control

Abstract

The application discloses an engineering strain of Beauveria bassiana expressing Hv1a toxin and a construction method and application thereof, and belongs to the technical field of bioengineering. The application takes Beauveria bassiana ARSEF 2860 as a host cell, replaces a natural signal peptide of an Hv1a gene with a chitinase Bbchit1 signal peptide of the Beauveria bassiana, and constructs the engineering strain Bb2860-Hv1a through a conidial spore competent mediated transformation. The engineering strain realizes efficient secretion expression of the Hv1a protein on the premise of maintaining normal growth, sporulation capacity and genetic stability of more than 10 generations of the wild type strain. The application fuses a double mechanism of fungal body wall invasion and rapid toxic killing of neurotoxin, widens a target spectrum of the Beauveria bassiana, and provides an efficient and safe biological technology approach for green prevention and control of agricultural pests.

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