Microfluidic reagent card

Abstract

The utility model belongs to the technical field of biological detection, and discloses a microfluidic reagent card which comprises a reagent card body, a microfluidic channel is arranged in the reagent card body, a marking area, a testing area and a quality control area are sequentially arranged in the microfluidic channel, a marking antibody is arranged in the marking area, a capture antibody is arranged in the testing area, and a quality control antibody is arranged in the quality control area. The head end and the tail end of each micro-channel are respectively provided with a liquid injection hole and an air hole, the plurality of micro-channels can detect different objects to be detected, the plurality of micro-channels share the same liquid injection hole, the air holes located at the tail ends of the plurality of micro-channels are located on the same circumference with the liquid injection hole as the circle center, and the shapes of the micro-channels are the same. The microfluidic reagent card can be used for simultaneously measuring various to-be-detected objects, and is high in detection efficiency and simple to operate.

Description

Technical Field

[0001] The utility model relates to the technical field of biological detection, in particular to a microfluidic reagent card. Background Art

[0002] The dairy product testing process involves many types of analytes, such as veterinary drug residues, pesticide residues, heavy metals, and microorganisms. In order to ensure the freshness of dairy products, the initial screening of various analytes in dairy products should be completed efficiently, quickly, and accurately.

[0003] Currently, colloidal gold detection reagent cards or microfluidic reagent cards are usually used for the initial screening of dairy product testing. However, the above two methods can only detect one analyte each time, requiring a large sample volume and a long detection time. Utility Model Content

[0004] The utility model aims to provide a microfluidic reagent card, which can detect a variety of test objects at the same time, has high detection efficiency and uses less sample.

[0005] To achieve this purpose, the utility model adopts the following technical solutions:

[0006] A microfluidic reagent card comprises a reagent card body, wherein a microfluidic channel is arranged in the reagent card body, wherein a labeling area, a test area and a quality control area are sequentially arranged in the microfluidic channel, wherein the labeling area is provided with a labeling antibody, the test area is provided with a capture antibody, and the quality control area is provided with a quality control antibody, wherein the first and last ends of the microfluidic channel are respectively provided with an injection hole and an air vent, wherein a plurality of microfluidic channels are provided, and each of the microfluidic channels can detect different analytes, wherein the plurality of microfluidic channels share the same injection hole, and the air vents at the tail ends of the plurality of microfluidic channels are on the same circumference with the injection hole as the center, and the shapes of the microfluidic channels are the same.

[0007] Preferably, the microfluidic channel is configured as a serpentine channel.

[0008] Preferably, the plurality of microchannels are distributed in a ring array.

[0009] Preferably, the injection hole and the vent hole are sealed with a cover.

[0010] Preferably, the sealing cover is a waterproof tape.

[0011] Preferably, the labeled antibody is fixed to the labeling area via a NC membrane.

[0012] Preferably, the surfaces of the quality control area and the test area are both provided with affinity layers for fixing the quality control antibody and the capture antibody.

[0013] Preferably, the reagent card body comprises a substrate and a cover plate, and the cover plate and the substrate are thermally pressed and bonded.

[0014] Preferably, a plurality of microfluidic grooves are provided on the substrate, each of the microfluidic grooves having an opening toward the cover plate, the cover plate blocks the opening to form the microfluidic channel, the head ends of the plurality of microfluidic grooves are connected via an injection groove, the cover plate is provided with an injection port at a position corresponding to the injection groove, and the injection port and the injection groove together form the injection hole.

[0015] Preferably, the microfluidic channel has a depth of 1 mm and a width of 1-2 mm.

[0016] Beneficial effects of the utility model:

[0017] The microfluidic reagent card of the utility model is provided with a plurality of microchannels connected to the same injection hole. Under the capillary action of the microchannels, the test sample can flow through the marking area, the test area and the quality control area in the microchannels in sequence, and independently complete the detection of multiple objects to be tested, with high detection efficiency and simple operation. Moreover, since the length and shape of each microchannel are the same, the time required to detect various objects to be tested is the same, and the required detection time is short. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural schematic diagram of a microfluidic reagent card in an embodiment of the utility model.

[0019] In the figure:

[0020] 1. Microfluidic channel; 11. Marking area; 12. Testing area; 13. Quality control area; 2. Liquid injection hole; 3. Ventilation hole. DETAILED DESCRIPTION

[0021] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention, rather than all structures, are shown in the accompanying drawings.

[0022] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0023] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0024] In the description of this embodiment, the terms "upper", "lower", "left", "right", etc., and the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of description and simplified operation, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used to distinguish in the description and have no special meaning.

[0025] refer to Figure 1 As shown, a microfluidic reagent card is proposed in an embodiment of the utility model, including a reagent card body, a plurality of microchannels 1 are arranged in the reagent card body, and the first and last ends of the microchannels 1 are connected to the outside world through the injection hole 2 and the air vent 3 respectively. A plurality of microchannels 1 share one injection hole 2, that is to say, a plurality of microchannels 1 are arranged around one injection hole 2. The microchannel 1 is sequentially provided with a labeling area 11, a test area 12 and a quality control area 13 from one end connected to the injection hole 2 to one end of the air vent 3, wherein the labeling area 11 is provided with an antibody (hereinafter referred to as a labeling antibody) that can bind to the analyte and is labeled with a signal particle, the test area 12 is provided with another antibody (hereinafter referred to as a capture antibody) that can bind to the analyte, and the quality control area 13 is provided with an antibody (hereinafter referred to as a quality control antibody) that can bind to the gold-labeled antibody, and each microchannel 1 can detect different analytes. It should be noted that, in order to ensure the synchronization and accuracy of the test, the air holes 3 are located on the same circle with the injection hole 2 as the center, and the shapes of the microchannels 1 are the same.

[0026] The above-mentioned microfluidic reagent card is provided with a plurality of microfluidic channels 1 connected to the same injection hole 2 on the reagent card body, and each microfluidic channel 1 is provided with different antibodies according to the difference of the analyte. Therefore, after the test sample is injected into the injection hole 2, under the capillary action of the microfluidic channel 1, the test sample can flow into each microfluidic channel 1 from the injection hole 2 and flow through the corresponding marking area 11, the test area 12 and the quality control area 13 in sequence, and the simultaneous detection of different analytes in the test sample can be realized by a single sample addition operation, and the detection efficiency is high. Moreover, since the shape and length of each microfluidic channel 1 are all the same, the reaction time of different analytes in the microfluidic channel 1 is also the same, which reduces the detection time and also improves the accuracy of the test. As for the specific number of microfluidic channels 1, it can be set to 3, 4 or 5, which is not specifically limited here.

[0027] It is understandable that in order to allow the test sample to fully react with each antibody in the microfluidic channel 1, the length of the microfluidic channel 1 cannot be shorter than a certain length, and the size of the microfluidic reagent card is limited. Based on this, the microfluidic channel 1 in this embodiment is configured as a serpentine channel with a longer effective length. Specifically, each corner of the microfluidic channel 1 has a smooth transition.

[0028] In this embodiment, in order to fully utilize the space on the reagent card body, a plurality of microfluidic channels 1 are distributed in a ring array.

[0029] When the test sample is added to the injection hole 2, some bubbles may be generated, affecting the flow of the test sample in the microfluidic channel 1. Therefore, a plurality of conical protrusions (not shown in the figure) are provided at the bottom of the injection hole 2, and the conical protrusions can puncture the bubbles generated during the sample addition process. More specifically, the plurality of conical protrusions are respectively provided in a one-to-one correspondence with the inlets of the plurality of microfluidic channels 1. At this time, the conical protrusions can block the unpunctured bubbles from flowing in the direction of the microfluidic channel 1. It can be understood that in order to prevent the conical protrusions from affecting the flow of the test sample into the microfluidic channel 1, there is a gap between the conical protrusions and the inlet of the microfluidic channel 1.

[0030] In this embodiment, the reagent card body includes a substrate and a cover plate, and the substrate and the cover plate are enclosed together to form a microfluidic channel 1. Specifically, a plurality of open microfluidic channels are provided on the side of the substrate facing the cover plate, and the head ends of the microfluidic channels are connected through a liquid injection groove. A liquid injection port is provided on the cover plate at a position corresponding to the liquid injection groove, and a vent hole 3 is provided at a position corresponding to the tail end of the microfluidic channel 1. The cover plate is hot-pressed and bonded to the substrate at 100°C, and the microfluidic channel 1 is formed after the opening of the microfluidic channel is blocked. At the same time, the liquid injection port is coaxial with the liquid injection groove to form a liquid injection hole 2 connecting the microfluidic channel 1 and the outside.

[0031] The microfluidic groove is processed by a 1w CO2 laser engraving machine, and the engraved microfluidic groove is 1mm deep and 1-2mm wide. The injection hole 2 is set as a circular hole with a diameter of 5mm, and the air hole 3 is set as a circular hole with a diameter of 0.5mm. In order to ensure the structural strength of the microfluidic reagent card, the base plate is made of a 3mm thick acrylic plate, and the cover plate is made of a 2mm thick acrylic plate.

[0032] In this embodiment, the signal particles used to label the antibody can be one or more of colloidal gold, biotin or fluorescent microspheres. It should be emphasized here that if the signal particles are colloidal gold, the labeled antibody is fixed to the labeling area 11 through a nitrocellulose film (NC membrane). When the test sample flows through the NC membrane, the labeled antibody binds to the analyte and continues to flow along the microchannel 1 with the test sample. When the signal particles are biotin or fluorescent microspheres, the labeled antibody can be directly fixed to the labeling area 11 by drying.

[0033] Affinity layers are provided on the surfaces of the detection zone and the quality control zone 13 to fix the capture antibody and the quality control antibody to prevent the capture antibody and the quality control antibody from flowing with the test sample. Specifically, the affinity layers on the surfaces of the detection zone and the quality control zone 13 are obtained by oxygen plasma treatment.

[0034] In this embodiment, in order to prevent foreign matter from entering the microchannel 1, the injection hole 2 and the vent hole 3 are both blocked with a cover (not shown in the figure). When in use, the cover is opened and 50ul of the sample to be tested is dripped into the injection hole 2. Exemplarily, the cover can be made of waterproof tape.

[0035] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For those skilled in the art, various obvious changes, readjustments and substitutions can be made without departing from the scope of protection of the present invention. It is not necessary and impossible to list all implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the scope of protection of the claims of the present invention.

Claims

1. A microfluidic reagent card, comprising a reagent card body, wherein a microfluidic channel (1) is arranged in the reagent card body, wherein a labeling area (11), a test area (12) and a quality control area (13) are arranged in sequence in the microfluidic channel (1), wherein the labeling area (11) is provided with a labeling antibody, the test area (12) is provided with a capture antibody, and the quality control area (13) is provided with a quality control antibody, and the first and last ends of the microfluidic channel (1) are respectively provided with a liquid injection hole (2) and a vent hole (3), characterized in that: The microfluidic channels (1) are provided with a plurality of them, and each of the microfluidic channels (1) can detect different analytes. The plurality of microfluidic channels (1) share the same injection hole (2), and the air holes (3) located at the tail ends of the plurality of microfluidic channels (1) are located on the same circumference with the injection hole (2) as the center, and the shapes of the microfluidic channels (1) are the same.

2. The microfluidic reagent card according to claim 1, characterized in that: The microfluidic channel (1) is configured as a serpentine channel.

3. The microfluidic reagent card according to claim 1, characterized in that: The plurality of microchannels (1) are distributed in a ring array.

4. The microfluidic reagent card according to claim 1, characterized in that: The liquid injection hole (2) and the air vent (3) are blocked with a cover.

5. The microfluidic reagent card according to claim 4, characterized in that: The sealing cover is a waterproof tape.

6. The microfluidic reagent card according to any one of claims 1 to 5, characterized in that: The labeled antibody is immobilized on the labeling area (11) via the NC membrane.

7. The microfluidic reagent card according to any one of claims 1 to 5, characterized in that: The surfaces of the quality control area (13) and the test area (12) are both provided with affinity layers for fixing the quality control antibody and the capture antibody.

8. The microfluidic reagent card according to any one of claims 1 to 5, characterized in that: The reagent card body comprises a substrate and a cover plate, and the cover plate and the substrate are thermally pressed and bonded.

9. The microfluidic reagent card according to claim 8, characterized in that: The substrate is provided with a plurality of microfluidic grooves, each of which has an opening facing the cover plate, and the cover plate blocks the opening to form the microfluidic channel (1). The head ends of the plurality of microfluidic grooves are connected via a liquid injection groove, and the cover plate is provided with a liquid injection port at a position corresponding to the liquid injection groove, and the liquid injection port and the liquid injection groove together form the liquid injection hole (2).

10. The microfluidic reagent card according to claim 9, characterized in that: The microfluidic channel has a depth of 1 mm and a width of 1-2 mm.

Images