Low-power scanning objective lens suitable for AI chromosome karyotype analysis

Abstract

The utility model provides a low-power scanning objective lens suitable for AI chromosome karyotype analysis. The low-power scanning objective lens suitable for AI karyotype analysis comprises a first lens, a second lens, a third lens, a fourth lens and a fifth lens which are sequentially arranged on the same optical axis, the second lens and the third lens are doubled to form a first lens group, and the fourth lens and the fifth lens are doubled to form a second lens group; the front surface of the first lens is a concave surface, the rear surface of the first lens is a convex surface, the front surface of the first lens group is a concave surface, the rear surface of the first lens group is a convex surface, the front surface of the second lens group is a convex surface, the rear surface of the second lens group is a convex surface, and the front surface of the sixth lens is a convex surface, and the rear surface of the sixth lens is a concave surface; the axial distance from the front surface of the first lens to the rear surface of the sixth lens is 44.5 mm. The low-power scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model solves the problem that a karyotype graph shot by an existing objective lens is generally not extremely high in definition, so that misjudgment of an AI algorithm is easily caused.

Description

Technical Field

[0001] The utility model relates to the field of objective lenses, in particular to a low-power scanning objective lens suitable for AI chromosome karyotype analysis. Background Art

[0002] In common automated chromosome karyotype analysis equipment, a 10x objective lens is usually used to scan the karyotype slide to find the low-magnification mitosis, and then a 100x oil lens is used to take high-magnification photos to obtain analyzable karyotype mitosis. Each slide usually scans thousands to tens of thousands of karyotypes, but only dozens of mitosis are suitable for analysis.

[0003] Traditional karyotype analysis equipment cannot distinguish whether the dispersion, overlap, and length of low-magnification divisions are suitable for analysis. Therefore, it takes a lot of time to wait until the 100x karyotype division shooting is completed before screening them one by one, which takes a lot of time.

[0004] With the development of AI technology, low-magnification splitting phase images taken with a 10x objective lens can be evaluated by real-time AI algorithms. The degree of dispersion, overlap, and length can be used to determine whether they can be used as analyzable splitting phases. Giving priority to shooting with a 100x objective lens can greatly shorten the analysis time. Figure 1 This is a metaphase karyotype division image of chromosomes taken with a 10x objective lens. Figure 2 The split phases are extracted from the 10x image and graded using a specific AI algorithm.

[0005] The existing commercial 10x objective lens has a NA of 0.25 and is designed with a 0.17 cover lens as standard. The chromosome karyotype sample slide does not come with a cover lens. The karyotype images taken with the current commercial objective lens are usually not clear enough, which can easily cause misjudgment by the AI ​​algorithm. Utility Model Content

[0006] In order to solve the problem that the karyotype images taken by existing objective lenses are usually not clear enough and easily cause misjudgment of AI algorithms, the utility model provides a low-magnification scanning objective lens suitable for AI chromosome karyotype analysis that solves the above problem.

[0007] A low-power scanning objective lens suitable for AI chromosome karyotype analysis, comprising a first lens to a sixth lens arranged in sequence on the same optical axis, wherein the second lens and the third lens are double-glued to form a first lens group, and the fourth lens and the fifth lens are double-glued to form a second lens group; the front surface of the first lens is concave and the rear surface is convex, the front surface of the first lens group is concave and the rear surface is convex, the front surface of the second lens group is convex and the rear surface is convex, and the front surface of the sixth lens is convex and the rear surface is concave; the axial distance from the front surface of the first lens to the rear surface of the sixth lens is 44.5 mm, the distance between the rear surface of the first lens and the front surface of the first lens group is 1 mm, the distance between the rear surface of the first lens group and the front surface of the second lens group is 2.5 mm, and the distance between the rear surface of the second lens group and the front surface of the sixth lens is 19.5 mm; the center thickness of the first lens is 2 mm, the center thickness of the first lens group is 5.5 mm, the center thickness of the second lens group is 6 mm, and the center thickness of the sixth lens is 6.5 mm.

[0008] In a preferred embodiment of the low-power scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model, the glass parameters of the first lens to the sixth lens are a refractive index between 1.5 and 1.8, and an Abbe number between 41 and 60. The refractive index of the first lens is 1.55 and the Abbe number is 86.2, the refractive index of the first lens group is 1.71 and the Abbe number is 67.5, the refractive index of the second lens group is 1.80 and the Abbe number is 52.3, and the refractive index of the sixth lens is 1.59 and the Abbe number is 84.5.

[0009] In a preferred embodiment of the low-power scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model, the working wavelength is the visible light band, the parfocal distance is 60mm, the NIKON upright optical system is matched, and the magnification is 10 times. The working distance from the object plane 0 to the front surface of the first lens is 15.5mm. The surfaces of the first lens to the sixth lens are all spherical, and the intervals between adjacent lenses are air.

[0010] In a preferred embodiment of the low-power scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model, within the full field of view, the maximum distortion does not exceed 1%, the vertical axis chromatic aberration does not exceed 6 microns, the root mean square value of the image point diffusion spot radius of each field of view is better than 4 microns, and the modulation transfer function value at the cutoff frequency of 20 line pairs per millimeter is better than 0.2.

[0011] Compared with the prior art, the low-magnification scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model has the following beneficial effects:

[0012] 1. The NA value increases, the low-magnification karyotype becomes clearer, and the detection rate of high-quality karyotype is improved;

[0013] 2. The working distance of NIKON's commonly used 10x objective lens is 7-10mm. During the automatic chromosome analysis process, the objective lens is automatically switched, which will be contaminated by the lens oil during long-term use. The 15.5mm large working distance design fundamentally eliminates the possibility of lens oil contamination;

[0014] 3. The objective lens is cost-effective, can be commercialized, and is suitable for rapid promotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a metaphase karyotype division phase image of chromosomes taken with a 10x objective lens;

[0016] Figure 2 Yes Figure 1 Schematic diagram after AI algorithm classification;

[0017] Figure 3 It is a structural schematic diagram of a low-power scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0019] Embodiment 1:

[0020] See also Figure 3 , is a schematic structural diagram of a low-power scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model.

[0021] The low-power scanning objective lens suitable for AI chromosome karyotype analysis includes a first lens 11, a second lens 21, a third lens 22, a fourth lens 31, a fifth lens 32 and a sixth lens 41 arranged in sequence along the same optical axis. The second lens 21 and the third lens 22 are double-glued to form a first lens group 2, and the fourth lens 31 and the fifth lens 32 are double-glued to form a second lens group 3. The first lens 11 to the sixth lens 41 are all made of crown glass and flint glass. The front surface of the first lens 11 is treated with a coating process to have better dust and dirt resistance.

[0022] The refractive index of the first lens is 1.55 and the Abbe number is 86.2, the refractive index of the first lens group is 1.71 and the Abbe number is 67.5, the refractive index of the second lens group is 1.80 and the Abbe number is 52.3, and the refractive index of the sixth lens is 1.59 and the Abbe number is 84.5. All of them are optical glasses with high cost performance and easy to purchase, suitable for mass production.

[0023] The front surface of the first lens 11 is concave and the rear surface is convex, the front surface of the first lens group 2 is concave and the rear surface is convex, the front surface of the second lens group 3 is convex and the rear surface is convex, and the front surface of the sixth lens 41 is convex and the rear surface is concave.

[0024] The working distance range of the objective lens from the object plane 0 to the front surface of the first lens 11 is 15.5 mm, which is larger than the default working distances of 7 mm and 10 mm of the NIKON series objective lenses. It is less likely to be contaminated by specimen immersion oil and is very suitable for low-magnification scanning of chromosome karyotypes.

[0025] The surfaces of the first lens 11 to the sixth lens 41 are all spherical, and the intervals between adjacent lenses are air. The axial distance from the front surface of the first lens 11 to the rear surface of the sixth lens 41 is 44.5 mm, the distance between the rear surface of the first lens 11 and the front surface of the first lens group 2 is 1 mm, the distance between the rear surface of the first lens group 2 and the front surface of the second lens group 3 is 2.5 mm, and the distance between the rear surface of the second lens group 3 and the front surface of the sixth lens 41 is 19.5 mm.

[0026] The center thickness of the first lens 11 is 2 mm, the center thickness of the first lens group 2 is 5.5 mm, the center thickness of the second lens group 3 is 6 mm, and the center thickness of the sixth lens 41 is 6.5 mm.

[0027] Based on the above parameters, the technical indicators that should be met by the low-power scanning objective lens for AI chromosome karyotype analysis provided by the utility model are:

[0028] Visible light, bright field, 10x magnification, parfocal distance 60mm, object field of view 22, numerical aperture NA=0.3, matched with NIKON upright optical system, for chromosome samples, no 0.17mm cover design, the objective lens thread interface is set to M25.

[0029] The maximum distortion of the objective lens within the full field of view does not exceed 1%, the vertical axis chromatic aberration does not exceed 6 microns, and the flat field achromatic effect is good. The root mean square value of the image point dispersion spot radius in each field of view is better than 4 microns, and the imaging performance is good. The modulation transfer function value at the cutoff frequency of 20 line pairs per millimeter is better than 0.2, and is close to the diffraction limit.

[0030] Embodiment 2:

[0031] The same version of software and hardware (KaryoVision 2310.1087 version, KSA-800 chromosome scanning analysis system) was used, and 10x NIKON objective lens and the low-power scanning objective lens provided by the utility model for AI chromosome karyotype analysis were used to perform low-power scanning on 5 chromosome karyotype slides (random fixed areas). The excellent karyotypes identified by AI under different objective lenses were manually judged, and the total number of excellent karyotypes found by manual judgment and the number of errors in excellent karyotypes were counted. The results are as follows:

[0032]

[0033] The low-power scanning objective lens suitable for AI chromosome karyotype analysis provided by the utility model has a higher excellent karyotype discovery rate and a lower error rate under experimental conditions.

[0034] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A low-power scanning objective lens suitable for AI chromosome karyotype analysis, characterized in that: The invention comprises a first lens to a sixth lens which are arranged in sequence on the same optical axis, wherein the second lens and the third lens are double-cemented to form a first lens group, and the fourth lens and the fifth lens are double-cemented to form a second lens group; the front surface of the first lens is concave and the rear surface is convex, the front surface of the first lens group is concave and the rear surface is convex, the front surface of the second lens group is convex and the rear surface is convex, and the front surface of the sixth lens is convex and the rear surface is concave; the axial distance from the front surface of the first lens to the rear surface of the sixth lens is 44.5 mm, the distance between the rear surface of the first lens and the front surface of the first lens group is 1 mm, the distance between the rear surface of the first lens group and the front surface of the second lens group is 2.5 mm, and the distance between the rear surface of the second lens group and the front surface of the sixth lens is 19.5 mm; the center thickness of the first lens is 2 mm, the center thickness of the first lens group is 5.5 mm, the center thickness of the second lens group is 6 mm, and the center thickness of the sixth lens is 6.5 mm.

2. The low-power scanning objective lens suitable for AI chromosome karyotype analysis according to claim 1, characterized in that: The glass parameters of the first lens to the sixth lens are a refractive index between 1.5 and 1.8, and an Abbe number between 41 and 60.

3. The low-power scanning objective lens suitable for AI chromosome karyotype analysis according to claim 2, characterized in that: The refractive index of the first lens is 1.55 and the Abbe number is 86.2, the refractive index of the first lens group is 1.71 and the Abbe number is 67.5, the refractive index of the second lens group is 1.80 and the Abbe number is 52.3, and the refractive index of the sixth lens is 1.59 and the Abbe number is 84.

5.

4. The low-power scanning objective lens suitable for AI chromosome karyotype analysis according to claim 1, characterized in that: The working wavelength is in the visible light band, the parfocal distance is 60mm, it is matched with the NIKON upright optical system, and the magnification is 10 times.

5. The low-power scanning objective lens suitable for AI chromosome karyotype analysis according to claim 1, characterized in that: The working distance from the object plane 0 to the front surface of the first lens is 15.5 mm.

6. The low-power scanning objective lens suitable for AI chromosome karyotype analysis according to claim 1, characterized in that: The surfaces of the first lens to the sixth lens are all spherical surfaces, and the intervals between adjacent lenses are air.

7. The low-power scanning objective lens suitable for AI chromosome karyotype analysis according to claim 1, characterized in that: Within the full field of view, the maximum distortion does not exceed 1%, the vertical axis chromatic aberration does not exceed 6 microns, the root mean square value of the image point dispersion radius in each field of view is better than 4 microns, and the modulation transfer function value at the cutoff frequency of 20 line pairs per millimeter is better than 0.2.

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