Multi-mode optical imaging equipment

Abstract

The utility model relates to a multi-mode optical imaging device which comprises a machine body, a motion system, a three-dimensional imaging module, a fluorescence imaging module and a data processing module. The machine body comprises a base and a detection platform; the motion system is connected with the three-dimensional imaging module and the fluorescence imaging module so as to drive the three-dimensional imaging module and the fluorescence imaging module to be aligned with a sample to-be-detected position on the detection platform; and the data processing module is connected with the motion system, the three-dimensional imaging module and the fluorescence imaging module. The system has the advantage of performing multi-dimensional imaging on a sample.

Description

Technical Field

[0001] The utility model relates to the technical field of ophthalmic imaging, in particular to a multi-modal optical imaging device. Background Art

[0002] Imaging the local anatomical structure of the fundus (retina or fundus) is an important means for diagnosing many eye diseases. Currently, mice are the most commonly used animal model for ophthalmic diseases. Photographing the mouse fundus is an indispensable means for scientific research. It can evaluate the posterior segment of the eyeball, including the vitreous body, optic disc, nerves and blood vessels on the retina, and provides a research basis for the study of the pathogenesis of many fundus diseases and the improvement of treatment methods.

[0003] However, in actual operation, existing fundus imaging equipment can only provide surface information about the mouse eye, and cannot achieve multi-dimensional imaging such as three-dimensional OCT structural imaging, blood flow imaging, autofluorescence imaging, etc. in the mouse eye, and thus cannot provide sufficient information to diagnose certain diseases. Summary of the invention

[0004] The technical problem to be solved by the utility model is to overcome the defects in the prior art, thereby providing a multi-modal optical imaging device.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A multimodal optical imaging device comprises: a body, a motion system, a three-dimensional imaging module, a fluorescence imaging module and a data processing module; the body comprises a base and a detection platform; the motion system connects the three-dimensional imaging module and the fluorescence imaging module to drive the three-dimensional imaging module and the fluorescence imaging module to align with a sample to be measured position located on the detection platform; the data processing module connects the motion system, the three-dimensional imaging module and the fluorescence imaging module.

[0007] Preferably, the motion system includes an X-axis sliding module, a Z-axis lifting platform, an A-axis rotating table and a C-axis rotating platform; the X-axis sliding module is slidably installed on the base; the Z-axis lifting platform is vertically slidably installed on the X-axis sliding module; the A-axis rotating table is rotatably installed on the Z-axis lifting platform and fixedly connected to the C-axis rotating platform; the three-dimensional imaging module and the fluorescent imaging module are rotatably installed on the C-axis rotating platform.

[0008] Preferably, the three-dimensional imaging module includes an OCT module and a high-power lens; wherein the OCT module includes a light engine module and a scanning probe, the scanning probe includes a two-dimensional high-speed scanning galvanometer XY and a scanning lens; the high-power lens is located between the scanning lens and the sample to be tested position.

[0009] Preferably, the OCT module is configured as a SS-OCT module or a SD-OCT module.

[0010] Preferably, the two-dimensional high-speed scanning galvanometer XY comprises a reflector X and a reflector Y, and the reflector Y is configured as a dichroic mirror.

[0011] Preferably, the light engine module comprises an electrically controlled reference arm, and the adjustable optical path range of the electrically controlled reference arm is not less than 80 mm.

[0012] Preferably, the data processing module includes a control component and an acquisition processing module; the control component is used to control the movement of the motion system to adjust the positions of the three-dimensional imaging module and the fluorescence imaging module; the acquisition processing module is used to display the 2D data or 3D data input by the OCT module, and the imaging data of the fluorescence imaging module in real time.

[0013] Preferably, the fluorescence imaging module comprises an excitation light source, an excitation light filter, a dichroic mirror, an emission light filter, and an imaging camera.

[0014] Compared with the prior art, the beneficial effect of the present invention is that the multimodal optical imaging device provided by the present invention can freely switch between three-dimensional OCT structural imaging, blood flow imaging, and autofluorescence imaging in the mouse eye by setting a three-dimensional imaging module and a fluorescence imaging module on the same device, and the three-dimensional imaging module and the fluorescence imaging module can be driven by a motion system. In addition, in terms of function, it can realize real-time 1D, 2D and 3D OCT structural and blood flow imaging, as well as multi-dimensional imaging such as two-dimensional fluorescence imaging. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 This is a structural schematic diagram provided in one embodiment of the present utility model.

[0017] Figure 2 for Figure 1 Schematic top view of .

[0018] Figure 3 for Figure 1 Schematic side view of .

[0019] Description of reference numerals:

[0020] 100. Body; 101. Base; 102. Detection platform; 103. Slide groove; 104. Interface; 200. Motion system; 201. X-axis sliding module; 202. Z-axis lifting platform; 203. A-axis rotating table; 204. C-axis rotating platform; 205. Column; 300. 3D imaging module; 400. Fluorescence imaging module. DETAILED DESCRIPTION

[0021] The technical solution of the utility model will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] See also Figures 1 to 3 The embodiment of the utility model provides a multimodal optical imaging device, including: a body 100, a motion system 200, a three-dimensional imaging module 300, a fluorescent imaging module 400 and a data processing module (not shown in the figure). The body 100 includes a base 101 and a detection platform 102. The detection platform 102 is used to fix a sample, such as a mouse. For the convenience of description, the sample in this embodiment is described by taking a mouse as an example, and the position of the sample to be tested is the mouse eye.

[0025] In order to facilitate the flexible adjustment of the detection platform 102, so that the mouse eyes on the detection platform 102 can be aligned with the three-dimensional imaging module 300 and the fluorescent imaging module 400, in this embodiment, the detection platform 102 is movably mounted on the base 101, and can move along three axes on the base 101, that is, it can slide on the horizontal plane where the base 101 is located, and can also move in a direction perpendicular to the horizontal plane where the base 101 is located. Of course, in other embodiments, the detection platform 102 can also be directly fixed on the base 101, and only the motion system 200 is used to drive the three-dimensional imaging module 300 and the fluorescent imaging module 400 to align the mouse eyes.

[0026] In order to facilitate the monitoring of experimental data and motion state, so that the three-dimensional imaging module 300 and the fluorescent imaging module 400 are accurately aligned with the mouse eye (the sample to be tested position located on the detection platform 102), in this embodiment, the data processing module connects the motion system 200, the three-dimensional imaging module 300 and the fluorescent imaging module 400 to realize data transmission. Specifically, the data processing module is used to control the movement of the motion system 200, and the motion system 200 is connected to the three-dimensional imaging module 300 and the fluorescent imaging module 400 to drive the three-dimensional imaging module 300 and the fluorescent imaging module 400 to align with the sample to be tested position located on the detection platform 102; the data processing module is also used to collect data from the motion system 200, the three-dimensional imaging module 300 and the fluorescent imaging module 400.

[0027] Specifically, in order to realize the observation of the three-dimensional imaging module 300 and the fluorescent imaging module 400 at any angle, in this embodiment, the motion system 200 includes an X-axis sliding module 201, a Z-axis lifting platform 202, an A-axis rotating platform 203 and a C-axis rotating platform 204. Among them, the X-axis sliding module 201 is slidably installed on the slide rail groove 103 provided on the base 101, and the Z-axis lifting platform 202 is vertically slidably installed on the column 205 provided on the X-axis sliding module 201; the A-axis rotating platform 203 is rotatably installed on the Z-axis lifting platform 202 and is fixedly connected to the C-axis rotating platform 204; the three-dimensional imaging module 300 and the fluorescent imaging module 400 are rotatably installed on the C-axis rotating platform 204. When the motion system 200 is driven, the X-axis sliding module 201 can drive the Z-axis lifting platform 202, the A-axis rotating platform 203, the C-axis rotating platform 204, the three-dimensional imaging module 300 and the fluorescent imaging module 400 to slide horizontally along the direction of the slide rail groove 103, the Z-axis lifting platform 202 can drive the A-axis rotating platform 203, the C-axis rotating platform 204, the three-dimensional imaging module 300 and the fluorescent imaging module 400 to slide vertically along the direction of the column 205, and the A-axis rotating platform 203 can drive the C-axis rotating platform 204, the three-dimensional imaging module 300 and the fluorescent imaging module 400 to rotate synchronously. , the C-axis rotating platform 204 can drive the three-dimensional imaging module 300 and the fluorescent imaging module 400 to rotate synchronously. It is worth noting that there is no sequence requirement for the operation of the various structures of the motion system 200, and they can be adjusted according to usage requirements, so that the three-dimensional imaging module 300 and the fluorescent imaging module 400 can achieve three-dimensional translation and rotation with the help of the motion system 200, and then the position and angle of the three-dimensional imaging module 300 and the fluorescent imaging module 400 relative to the mouse eye can be accurately adjusted to achieve imaging of the structure and blood flow of the eye tissue, achieve label-free imaging of the mouse eye, and detect specific cell types.

[0028] In this embodiment, the three-dimensional imaging module 300 includes an OCT module and a high-power lens. In this embodiment, the high-power lens is an ophthalmic lens. The high-power lens is located between the OCT module and the position of the sample to be tested, so that the high-power lens and the OCT module can be used in conjunction. The high-power lens can be used to magnify the rat eye, making it easier for the OCT module to observe and study the structure and characteristics of the rat eye.

[0029] Specifically, the OCT module can be set as an SS-OCT module. In this case, the OCT module includes an optical engine module and a scanning probe, wherein the optical engine module includes a low-coherence light source, an interference module, an electrically controlled reference arm and a balanced photodetector, the low-coherence light source is a high-speed frequency-sweeping laser light source, and the optical path of the electrically controlled reference arm can be adjusted within a range of not less than 80 mm. The scanning probe includes a collimating mirror, a two-dimensional high-speed scanning galvanometer XY, a scanning lens and a monitoring camera. The scanning probe can accurately adjust its position and angle with the mouse eye through the motion system 200, so that the light beam entering the scanning probe reaches the two-dimensional high-speed scanning galvanometer XY after passing through the collimating mirror, and then reaches the mouse eye through the scanning lens, wherein the monitoring camera of the scanning probe can visually monitor the imaging field of view. The high-power lens is located between the scanning lens and the sample to be tested.

[0030] When in use, the light beam emitted by the high-speed frequency-sweeping laser source is split into two after passing through the 1×2 beam splitter of the interference module. One beam passes through the circulator of the interference module and enters the collimator, focusing lens and reflector of the electric control reference arm, and then returns along the original path. The returned light beam is output by the circulator of the interference module as a reference signal; the other beam passes through the circulator of the interference module and enters the scanning probe, and passes through the collimator, two-dimensional high-speed scanning galvanometer XY, scanning lens and high-power lens of the scanning probe to reach the sample, i.e. the mouse eye. The backscattered light of the sample returns along the original path and is output by the circulator as a sample signal. The reference signal and the sample signal interfere with each other through the 2×2 coupler of the interference module. The output signals of the 2×2 coupler enter the balanced photodetector respectively, and after the photoelectric signal conversion, the data is collected by the data processing module.

[0031] Furthermore, the OCT module can also be set as an SD-OCT module. In this case, the OCT module includes an optical engine module and a scanning probe. Among them, the optical engine module includes a low-coherence light source, an interference module, an electric reference arm and a spectrometer. The low-coherence light source is a broadband super-radiant light-emitting diode, and the optical path of the electric control reference arm can be adjusted within a range of not less than 80 mm. The scanning probe includes a collimating mirror, a two-dimensional high-speed scanning galvanometer XY, a scanning lens and a monitoring camera. The scanning probe can accurately adjust its position and angle with the mouse eye through the motion system 200, so that the light beam entering the scanning probe reaches the two-dimensional high-speed scanning galvanometer XY after the collimating mirror, and then reaches the mouse eye through the scanning lens, wherein the monitoring camera of the scanning probe can visually monitor the imaging field of view. The high-power lens is located between the scanning lens and the sample to be tested.

[0032] When in use, the light beam emitted by the broadband superluminescent diode is split into two after passing through the circulator and 2×2 coupler of the interference module. One beam enters the collimator, focusing lens and reflector of the electric control reference arm and returns to the original path as a reference signal; the other beam enters the scanning probe, passes through the collimator, two-dimensional high-speed scanning galvanometer XY, scanning lens and high-power lens to reach the sample, and the backscattered light of the sample returns to the original path as a sample signal. The reference signal and the sample signal interfere with each other through the 2×2 coupler of the interference module, and the output signal enters the spectrometer, and the data is collected by the data processing module.

[0033] Furthermore, the two-dimensional high-speed scanning galvanometer XY includes a reflector X and a reflector Y, wherein the reflector Y can be a dichroic mirror, which can be compatible with a surveillance camera to display the imaging field of view, and can also be used as an optical design interface integrated with the fluorescent imaging module 400.

[0034] See also Figure 1 and Figure 3 In this embodiment, the data processing module is connected to the motion system 200, the three-dimensional imaging module 300 and the fluorescence imaging module 400 through the interface 104 arranged on the body 100. On the one hand, it can control the movement of the motion system 200 to drive the three-dimensional imaging module 300 and the fluorescence imaging module 400 to align with the sample to be tested position located on the detection platform 102; on the other hand, it can collect data from the motion system 200, the three-dimensional imaging module 300 and the fluorescence imaging module 400.

[0035] Specifically, the data processing module includes a control component and an acquisition processing module; the control component is used to control the movement of the motion system 200 to adjust the positions of the three-dimensional imaging module 300 and the fluorescence imaging module 400; the acquisition processing module is used to display in real time the 2D data or 3D data input by the OCT module and the imaging data of the fluorescence imaging module.

[0036] Furthermore, the acquisition and processing module consists of a high-speed data acquisition card, a high-throughput data and image processing workstation, etc. The selected high-speed data acquisition card obtains the signal of the balanced detector. A three-dimensional scanning experiment can generate up to hundreds of GB of data. In order to collect and store this data, the equipped server will use a high-throughput data acquisition card to collect this data and store it in an ultra-large capacity storage module, which can display and process 2D and 3D OCT data in real time.

[0037] In order to achieve label-free imaging of mouse eyes and detect specific cell types, in this embodiment, the fluorescence imaging module 400 includes an excitation light source, an excitation light filter, a dichroic mirror, an emission light filter, and an imaging camera.

[0038] From the above, it can be seen that this embodiment has at least the following advantages:

[0039] 1. By setting up a three-dimensional imaging module 300 and a fluorescence imaging module 400 on the same device and driving it by a motion system 200, the device can freely switch between three-dimensional OCT structural imaging, blood flow imaging, and autofluorescence imaging in the mouse eye. In terms of function, real-time 1D, 2D, and 3D OCT structural and blood flow imaging, as well as two-dimensional fluorescence imaging and video recording can be achieved.

[0040] 2. The three-dimensional imaging module 300 and the fluorescent imaging module 400 are driven by the motion system 200, and the motion system 200 includes an X-axis sliding module 201, a Z-axis lifting platform 202, an A-axis rotating platform 203 and a C-axis rotating platform 204, so that a variety of motion modes can be fitted, so that the three-dimensional imaging module 300 and the fluorescent imaging module 400 can observe the sample from any angle, and the alignment effect with the sample is better.

[0041] 3. The OCT module of the three-dimensional imaging module 300 includes a light engine module, which can provide high-speed laser scanning and support three-dimensional, non-destructive, high-speed scanning imaging of eye tissues. The scanning lens can be selected according to different imaging samples, and the imaging field of view can be visually monitored by the monitoring camera.

[0042] 4. The fluorescence imaging module 400 can perform autofluorescence imaging of the target area of ​​the mouse eye, as well as statistical analysis of the number of fluorophores and the fluorescence area in the imaging area, so as to achieve label-free imaging of the mouse eye and detect specific cell types.

[0043] 5. The data processing module can realize high-speed data acquisition, fast processing and 3D visualization. The data processing module supports fast data processing and storage of three-dimensional OCT data.

[0044] The above-mentioned implementation modes are only preferred implementation modes of the present invention, and cannot be used to limit the protection scope of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A multimodal optical imaging device, characterized in that: include: Airframe, motion system, 3D imaging module, fluorescence imaging module and data processing module; The fuselage includes a base and a detection platform; The motion system is connected to the three-dimensional imaging module and the fluorescent imaging module to drive the three-dimensional imaging module and the fluorescent imaging module to align with the sample to be tested position located on the detection platform; The data processing module is connected to the motion system, the three-dimensional imaging module and the fluorescence imaging module.

2. A multimodal optical imaging device according to claim 1, characterized in that: The motion system includes an X-axis sliding module, a Z-axis lifting platform, an A-axis rotating platform and a C-axis rotating platform; The X-axis sliding module is slidably mounted on the base; The Z-axis lifting platform is vertically slidably mounted on the X-axis sliding module; The A-axis rotating platform is rotatably mounted on the Z-axis lifting platform and is fixedly connected to the C-axis rotating platform; The three-dimensional imaging module and the fluorescent imaging module are rotatably mounted on the C-axis rotating platform.

3. A multimodal optical imaging device according to claim 1, characterized in that: The three-dimensional imaging module includes an OCT module and a high-power lens; Wherein, the OCT module includes an optical engine module and a scanning probe, and the scanning probe includes a two-dimensional high-speed scanning galvanometer XY and a scanning lens; The high-power lens is located between the scanning lens and the sample to-be-tested position.

4. A multimodal optical imaging device according to claim 3, characterized in that: The OCT module is configured as a SS-OCT module or a SD-OCT module.

5. A multimodal optical imaging device according to claim 3 or 4, characterized in that: The two-dimensional high-speed scanning galvanometer XY includes a reflector X and a reflector Y, and the reflector Y is configured as a dichroic mirror.

6. A multimodal optical imaging device according to claim 3, characterized in that: The optical engine module includes an electrically controlled reference arm, and the adjustable optical path range of the electrically controlled reference arm is not less than 80 mm.

7. The multimodal optical imaging device according to claim 3, characterized in that: The data processing module includes a control component and an acquisition processing module; The control component is used to control the motion system to adjust the positions of the three-dimensional imaging module and the fluorescent imaging module; The acquisition and processing module is used to display the 2D data or 3D data input by the OCT module and the imaging data of the fluorescence imaging module in real time.

8. The multimodal optical imaging device according to claim 1, characterized in that: The fluorescence imaging module includes an excitation light source, an excitation light filter, a dichroic mirror, an emission light filter, and an imaging camera.

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