Nucleic acid extraction assembly for nucleic acid extractor

Abstract

The utility model discloses a nucleic acid extraction component for a nucleic acid extractor, which comprises a nucleic acid extraction reagent groove support and a nucleic acid extraction reagent groove matched with a containing groove of the nucleic acid extraction reagent groove support in size, and a fixing device is arranged on the side wall of the nucleic acid extraction reagent groove support. The nucleic acid extraction reagent tank support can better fix the nucleic acid extraction reagent tank, provide stable support for the nucleic acid extraction reagent tank and prevent the reagent tank from shaking or inclining in the operation process, the nucleic acid extraction reagent tank can pre-package reagents required by the whole process of nucleic acid extraction, and nucleic acid extraction of 1-2 persons can be completed by matching with an automatic nucleic acid extraction instrument. And a plurality of nucleic acid extraction reagent tanks can be matched according to different fluxes to cope with more diversified extraction modes.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid molecule extraction and purification, and more specifically, to a nucleic acid extraction component for a nucleic acid extractor. Background Art

[0002] In the field of molecular diagnosis, the extraction of nucleic acid molecules is a key link. The widespread use of fully automatic nucleic acid extractors has greatly simplified the nucleic acid extraction process. Compared with traditional manual nucleic acid extraction, it has the advantages of saving time and labor and reducing exogenous contamination. However, during the use of the nucleic acid extractor, it is necessary to ensure that the position of the reagent tank is strictly fixed on the bracket to ensure uniform mixing of the reagents and prevent cross-infection between samples.

[0003] However, the reagent tank holder currently used in nucleic acid extraction instruments cannot stably fix the reagent tank. During actual use, the reagent tank often shakes or tilts, affecting the uniform mixing of reagents, thereby causing the purity of the extracted nucleic acid to be too low and unable to be used in the next experiment.

[0004] Moreover, the conventional consumables currently available on the market for nucleic acid extraction equipment are divided into deep-well plates and corresponding magnetic rod sleeves. The corresponding extraction fluxes of conventional deep-well plate reagent tanks are generally divided into 8, 16, 32 and 96 servings. If conventional extraction consumables are used to cope with the extraction flux of 1 to 7 servings, it will inevitably cause waste of reagents and consumables, and increase the cost of low-throughput nucleic acid extraction. Summary of the invention

[0005] The purpose of the utility model is to overcome the problem that the reagent tank bracket in the nucleic acid extraction instrument in the prior art cannot stably fix the reagent tank, and the conventional nucleic acid extraction consumables will inevitably cause waste of reagents and consumables when performing low-throughput nucleic acid extraction. A nucleic acid extraction component for a nucleic acid extraction instrument is provided, and the nucleic acid extraction reagent tank bracket can stably fix the nucleic acid extraction reagent tank. The nucleic acid extraction reagent tank can pre-package the reagents required for the entire nucleic acid extraction process to complete the nucleic acid extraction of 1 to 2 people, reduce the waste of consumables and reduce the cost of single-person extraction.

[0006] The specific technical solutions are as follows:

[0007] A nucleic acid extraction component for a nucleic acid extraction instrument, a nucleic acid extraction reagent tank bracket and a nucleic acid extraction reagent tank, wherein the nucleic acid extraction reagent tank bracket comprises a bottom surface and two opposite side walls, the nucleic acid extraction reagent tank bracket is provided with a receiving tank for receiving the nucleic acid extraction reagent tank, and the two walls of the nucleic acid extraction reagent tank bracket are respectively provided with fixing devices, the fixing devices clamp the two sides of the nucleic acid extraction reagent tank and are used to fix the nucleic acid extraction reagent tank in the receiving tank.

[0008] Preferably, the containing groove is arranged parallel to the bottom surface, and the size of the containing groove matches the nucleic acid extraction reagent groove.

[0009] Preferably, the bottom surface is a rectangular bottom surface.

[0010] Preferably, the fixing device comprises a claw member, and engaging portions engaged with the claw member are provided on both sides of the nucleic acid extraction reagent tank.

[0011] More preferably, the claw member comprises a claw portion engaged with the engaging portion and an elastic portion driving the claw portion to press the engaging portion.

[0012] Further more preferably, the claw portion and the elastic portion are integrally formed as a spring sheet.

[0013] Most preferably, the claw portion is provided with a conical protrusion having a first inclined surface and a second inclined surface.

[0014] More preferably, the claw member is connected to the side wall of the nucleic acid extraction reagent tank bracket through a fixing element.

[0015] More preferably, the claw member is detachably connected to the side wall of the nucleic acid extraction reagent tank bracket through a fixing element.

[0016] Specifically, the claw piece and the side wall of the nucleic acid extraction reagent tank bracket are both provided with mounting holes, and the fixing element passes through the mounting holes to connect the claw piece to the side wall of the nucleic acid extraction reagent tank bracket in a detachable manner.

[0017] Preferably, the fixing element is a rubber plug.

[0018] More preferably, the fixing element has a protruding structure, and the diameter of the protruding structure is larger than the diameter of the mounting hole, which is an interference fit.

[0019] As a reference for a specific implementation, the material of the clamping claw is 304 stainless steel, and the material of the fixing element is polyoxymethylene.

[0020] Preferably, the nucleic acid extraction reagent tank is provided with reinforcing ribs at both ends close to the nucleic acid extraction reagent tank bracket.

[0021] More preferably, the engaging portion is arranged on the reinforcing rib, and the claw member extends into the engaging groove to engage with the engaging portion.

[0022] Preferably, the engaging portion is arranged on one side of the reinforcing rib close to the bottom surface.

[0023] More preferably, one end of the reinforcing rib is flush with the open end of the nucleic acid extraction reagent tank.

[0024] As a reference for a specific implementation, the material of the bottom surface is polyoxymethylene.

[0025] As a reference for specific implementation, the bottom surface is provided with eight rows and six columns of receiving grooves arranged in an array. Correspondingly, the walls of the nucleic acid extraction reagent tank bracket are respectively provided with symmetrically distributed card grooves and elastic fixing devices. After the nucleic acid extraction reagent tank is placed in the receiving groove, the open end of the nucleic acid extraction reagent tank is flush with the height of the nucleic acid extraction reagent tank bracket or slightly higher than the nucleic acid extraction reagent tank bracket.

[0026] Preferably, the nucleic acid extraction reagent tank is 8 monomer reagent tanks arranged side by side.

[0027] Preferably, the reinforcing rib is parallel to the axial direction of the monomer reagent tank.

[0028] Preferably, a protrusion is provided on the outer side of the opening end of the nucleic acid extraction reagent tank.

[0029] As a reference for a specific implementation, the protruding points are arranged at the connection between the monomer reagent grooves.

[0030] As a reference for specific implementation, the material of the nucleic acid extraction reagent tank is polypropylene.

[0031] Preferably, the other end of the monomer reagent tank has a conical bottom.

[0032] Preferably, the nucleic acid extraction reagent tank contains a nucleic acid extraction reagent.

[0033] More preferably, the nucleic acid extraction reagent tank encapsulates one or more of a nucleic acid extraction reagent lysing solution, a washing solution and / or an elution solution.

[0034] Preferably, the volume of the monomer reagent tank is 2-2.2 ml.

[0035] Compared with the prior art, this new method has the following beneficial effects:

[0036] The utility model cooperates the claw parts on the nucleic acid extraction reagent tank bracket and the reinforcing ribs on the nucleic acid extraction reagent tank, so that the nucleic acid extraction reagent tank bracket can better fix the nucleic acid extraction reagent tank, provide stable support for the nucleic acid extraction reagent tank, prevent the reagent tank from shaking or tilting during operation, make the reagent tank more stable during the nucleic acid extraction process, avoid uneven mixing or leakage of nucleic acid extraction reagents, and reduce the movement of the reagent tank, thereby avoiding contamination and cross infection problems between samples, and further improve the purity of the extracted nucleic acid sample.

[0037] The nucleic acid extraction reagent tank bracket provided by the utility model has a simple structure and is easy to assemble and disassemble. It is applicable to common nucleic acid extraction instruments and is adapted to common nucleic acid extraction reagent tanks. It can be reused after being sterilized with ultraviolet light or alcohol, etc., thereby reducing the cost of consumables in the nucleic acid extraction process and can effectively prevent the reagent tank from accidentally falling or being damaged, thereby protecting the safety and integrity of the nucleic acid extraction equipment, helping to extend the service life of the nucleic acid extraction equipment, and reducing the cost of maintenance and replacement of the nucleic acid extraction equipment.

[0038] The nucleic acid extraction reagent tank provided by the utility model can pre-package the reagents required for the entire process of nucleic acid extraction, is suitable for common nucleic acid extraction reagent tanks, and can be used with automatic nucleic acid extraction instruments to complete the nucleic acid extraction of 1 to 2 people. It can also match multiple nucleic acid extraction reagent tanks according to different fluxes to cope with more diverse extraction methods. Reduce the waste of consumables and further reduce the cost of single-person extraction. Reinforcing ribs are provided on both sides of the nucleic acid extraction reagent tank to facilitate fixing the nucleic acid extraction reagent tank on the bracket of the nucleic acid extraction instrument, reduce the risk of the reagent tank falling off or overturning due to vibration or movement, and avoid uneven reagent infiltration or leakage due to looseness or instability. The protruding points on the nucleic acid extraction reagent tank can strengthen the adhesion between the heat-sealing film and the nucleic acid extraction reagent tank when pre-packaging the reagent, prevent liquid leakage, and make it easier to tear the heat-sealing film during actual use. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 It is a three-dimensional view of the utility model.

[0040] Figure 2 This is a three-dimensional view of the nucleic acid extraction reagent tank support described in the utility model.

[0041] Figure 3 It is a schematic diagram of the internal structure of the utility model.

[0042] Figure 4 for Figure 3 A partial enlarged view of position A.

[0043] Figure 5 This is a three-dimensional view of the nucleic acid extraction reagent tank described in the present invention.

[0044] Figure 6 This is a front view of the nucleic acid extraction reagent tank described in the utility model.

[0045] Figure 7 It is a cross-sectional view of the nucleic acid extraction reagent tank described in the present utility model.

[0046] In the figure: 1. Nucleic acid extraction reagent tank bracket; 11. Receiving tank; 121. Claw member; 1211. Claw portion; 1212. Elastic portion; 122. Fixing element; 123. Mounting hole; 13. Slot; 14. Bottom surface; 2. Nucleic acid extraction reagent tank; 22. Monomer reagent tank; 23. Reinforcing rib; 24. Engaging portion; 25. Protruding point. DETAILED DESCRIPTION

[0047] In order to make the technical problems solved by the utility model, the technical solutions adopted and the technical effects achieved clearer, the technical solutions of the embodiments of the utility model will be further described in detail below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the utility model.

[0048] Example 1 A nucleic acid extraction component for a nucleic acid extraction instrument

[0049] like Figure 1 to Figure 2 As shown, the utility model discloses a nucleic acid extraction component for a nucleic acid extraction instrument, comprising: a nucleic acid extraction reagent tank bracket (1) and a nucleic acid extraction reagent tank (2), wherein the nucleic acid extraction reagent tank (2) is 8 monomer reagent tanks (22) arranged side by side.

[0050] The nucleic acid extraction reagent tank support (1) comprises a rectangular bottom surface (14) and four side walls, the bottom surface (14) is provided with a receiving groove (11), a nucleic acid extraction reagent tank (2) is installed in the receiving groove (11), the nucleic acid extraction reagent tank (2) matches the size of the nucleic acid extraction reagent tank support (1), the nucleic acid extraction reagent tank (2) is provided with a clamping part (24) at both ends close to the nucleic acid extraction reagent tank support (1), and symmetrically distributed clamping grooves (13) and elastic fixing devices are provided on two opposite side walls, the nucleic acid extraction reagent tank (2) is provided with a clamping part (24) at both ends close to the nucleic acid extraction reagent tank support (1), and the nucleic acid extraction reagent tank (2) is provided with a clamping part (13) and an elastic fixing device at two opposite side walls, The reagent tank bracket (1) is connected to the nucleic acid extraction reagent tank (2) via an elastic fixing device. The elastic fixing device is a claw member (121) arranged on the side wall of the nucleic acid extraction reagent tank bracket (1). The claw member (121) comprises a claw portion (1211) and an elastic portion (1212) that drives the claw portion (1211) to press the clamping portion (24). The claw member (121) extends into the clamping slot (13) and contacts the clamping portion (24), thereby clamping the nucleic acid extraction reagent tank (2) on the nucleic acid extraction reagent tank bracket (1).

[0051] The claw member (121) is connected to the side wall of the nucleic acid extraction reagent tank bracket (1) via a fixing element (122); both the claw member (121) and the side wall of the nucleic acid extraction reagent tank bracket (1) are provided with mounting holes (123); the fixing element (122) passes through the mounting holes (123) to connect the claw member (121) to two opposite side walls of the nucleic acid extraction reagent tank bracket (1) in a detachable manner.

[0052] In this embodiment, the claw portion (1211) and the elastic portion (1212) are integrally formed into a spring piece with a conical protrusion having a first inclined surface and a second inclined surface, and the material is 304 stainless steel. The fixing element (122) is a rubber plug, and the material is polyoxymethylene (PMO). The rubber plug has a protrusion structure, and the diameter of the protrusion structure is larger than the diameter of the mounting hole (123), which is an interference fit. The nucleic acid extraction reagent tank bracket (1) is made of PMO, and the bottom surface (14) is provided with eight rows and six columns of receiving grooves (11) arranged in an array shape. The side walls of the nucleic acid extraction reagent tank bracket (1) at both ends of the receiving groove (11) are respectively provided with symmetrically distributed card grooves (13) and claw parts (121). The nucleic acid extraction reagent tank (2) has a conical bottom and is made of polypropylene. After the nucleic acid extraction reagent tank (2) is installed in the receiving groove (11), the opening end of the nucleic acid extraction reagent tank (2) is slightly higher than the nucleic acid extraction reagent tank bracket (1). The volume of the monomer reagent tank (22) is 2 to 2.2 ml.

[0053] When in use, the monomer reagent tank (22) is encapsulated with a nucleic acid extraction reagent.

[0054] The working principle of this embodiment is as follows: after the nucleic acid extraction reagent tank (2) is installed in the receiving tank (11), the conical protrusion on the claw member (121) extends into the slot (13) and contacts the engaging portion (24), thereby clamping the nucleic acid extraction reagent tank (2) on the nucleic acid extraction reagent tank bracket (1), thereby achieving the purpose of fixing the nucleic acid extraction reagent tank (2) on the nucleic acid extraction reagent tank bracket (1), preventing the nucleic acid extraction reagent tank (2) from loosening during use, and the experimenter only needs to move the claw member (121) to take the nucleic acid extraction reagent tank (2); the nucleic acid extraction reagent tank (2) is fixed to the nucleic acid extraction reagent tank bracket (1), and the nucleic acid extraction reagent tank (2) is prevented from loosening during use. The experimenter only needs to move the claw member (121) to take the nucleic acid extraction reagent tank (2); The process of extracting nucleic acid by the instrument includes one lysis, two to three washings and one elution step. When preparing the nucleic acid extraction reagent tank (2), the reagents required for different steps of nucleic acid extraction are pre-packaged in different monomer reagent tanks (22). The nucleic acid extraction reagent tank (2) can be sealed by using a heat-sealing film to prevent leakage of the reagent. The nucleic acid extraction component is placed in the nucleic acid extraction instrument, and the instrument is started to complete the nucleic acid extraction of 1 to 2 people. The same nucleic acid extraction reagent can also be pre-packaged in different monomer reagent tanks (22) to cope with conventional high-throughput extraction.

[0055] Example 2 A nucleic acid extraction component for a nucleic acid extraction instrument

[0056] like Figure 3 and 4 As shown, on the basis of Example 1, a reinforcing rib (23) is further provided on the nucleic acid extraction reagent tank (2), one end of the reinforcing rib (23) is flush with the open end of the nucleic acid extraction reagent tank (2) and is parallel to the axial direction of the monomer reagent tank (22), and the engaging portion (24) is provided on the reinforcing rib (23), and the claw member (121) extends into the slot (13) and engages with the engaging portion (24), and the engaging portion (24) is located on the side of the claw member close to the bottom surface (14).

[0057] The working principle of this embodiment is as follows: the claw member (121) on the side wall of the nucleic acid extraction reagent tank bracket (1) extends into the clamping groove (13) and is clamped with the clamping portion (24) on the reinforcing rib (23), forming a buckle for fixed connection, thereby fixing the nucleic acid extraction reagent tank (2) more firmly on the nucleic acid extraction reagent tank bracket (1), preventing the nucleic acid extraction reagent tank from loosening during use. The experimenter only needs to move the claw member (121) to take the nucleic acid extraction reagent tank (2).

[0058] Example 3 A nucleic acid extraction component for a nucleic acid extraction instrument

[0059] like Figure 5 , Figure 6 and Figure 7 As shown, based on Example 2, a protrusion (25) is provided on the outer side of the opening end of the monomer reagent tank (22).

[0060] In this embodiment, the protruding points (25) are arranged at the connection between the monomer reagent tanks (22), and the nucleic acid extraction reagent tank (2) is provided with 14 protruding points (25) in total.

[0061] The working principle of this embodiment is as follows: the protruding point (25) can cooperate with the heat-sealing film to strengthen the adhesion between the heat-sealing film and the nucleic acid extraction reagent tank when pre-packaging the reagent, thereby improving the sealing performance of the nucleic acid extraction reagent tank (2) after pre-packaging the reagent and preventing the reagent from leaking.

[0062] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A nucleic acid extraction component for a nucleic acid extraction instrument, comprising a nucleic acid extraction reagent tank support (1) and a nucleic acid extraction reagent tank (2), wherein the nucleic acid extraction reagent tank support (1) comprises a bottom surface (14) and two opposite side walls, and the nucleic acid extraction reagent tank support (1) is provided with a receiving tank (11) for receiving the nucleic acid extraction reagent tank (2), characterized in that: Fixing devices are respectively provided on the two walls of the nucleic acid extraction reagent tank support (1), and the fixing devices clamp the two sides of the nucleic acid extraction reagent tank (2) and are used to fix the nucleic acid extraction reagent tank (2) in the containing tank (11).

2. The nucleic acid extraction component according to claim 1, characterized in that: The fixing device comprises a claw member (121), and both sides of the nucleic acid extraction reagent tank (2) are provided with engaging portions (24) engaged with the claw member (121).

3. The nucleic acid extraction component according to claim 2, characterized in that: The clamping claw member (121) comprises a clamping claw portion (1211) clamped with the clamping portion (24) and an elastic portion (1212) driving the clamping claw portion (1211) to press the clamping portion (24).

4. The nucleic acid extraction component according to claim 3, characterized in that: The claw portion (1211) and the elastic portion (1212) are integrally formed into a spring sheet.

5. The nucleic acid extraction component according to claim 4, characterized in that: The claw portion (1211) is provided with a conical protrusion having a first inclined surface and a second inclined surface.

6. The nucleic acid extraction component according to claim 5, characterized in that: The claw member (121) is connected to the side wall of the nucleic acid extraction reagent tank support (1) via a fixing element (122).

7. The nucleic acid extraction component according to claim 2, characterized in that: The nucleic acid extraction reagent tank (2) is provided with reinforcing ribs (23) at both ends close to the nucleic acid extraction reagent tank bracket (1).

8. The nucleic acid extraction component according to claim 7, characterized in that: The clamping portion (24) is arranged on the reinforcing rib (23), and the clamping claw member (121) extends into the clamping groove (13) to be clamped with the clamping portion (24).

9. The nucleic acid extraction component according to claim 1, characterized in that: The nucleic acid extraction reagent tank (2) comprises eight single reagent tanks (22) arranged side by side.

10. The nucleic acid extraction component according to claim 1, characterized in that: A protruding point (25) is provided on the outer side of the opening end of the nucleic acid extraction reagent tank (2).

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