Reaction device for product recovery after solid-phase chemical synthesis

Abstract

The utility model provides a reaction device for recovering a product after solid-phase chemical synthesis, the reaction device comprises a reaction assembly and a driving assembly, the reaction assembly comprises a reactor filled with a reaction reagent and a temperature control unit for heating the reaction reagent in the reactor, and the temperature control unit is connected with the reactor; the driving assembly is located outside the reactor and connected with the reactor, and the driving assembly is used for driving liquid in the reactor to flow, so that the liquid can repeatedly scour the solid-phase carrier in the reactor. The reaction device provided by the utility model can improve the product recovery efficiency after solid-phase chemical synthesis.

Description

Technical Field

[0001] The present disclosure relates to the field of biochemical technology, and in particular to a reaction device for recovering products after solid phase chemical synthesis. Background Art

[0002] Solid phase chemical synthesis is a method of synthesizing compounds by connecting reactants to an insoluble solid phase carrier. This method can synthesize a variety of biomacromolecules, such as nucleic acids, polypeptides, etc. During solid phase chemical synthesis, the product is usually anchored to the solid phase carrier through a connecting arm, and there will be protecting groups on the product. Therefore, before harvesting the product, deprotection and connecting arm cleavage treatment are required. Among them, deprotection refers to removing the protecting group with the corresponding deprotecting group reagent. Breaking the connecting arm refers to cutting the connecting arm with the corresponding connecting arm cutting reagent. For example, during nucleic acid synthesis, a reagent (ammonia gas or ammonia water) can be used to deprotect the product and break the connecting arm. After deprotection and breaking the connecting arm, the product can be eluted and recovered, and the solid phase carrier is discarded.

[0003] In the related art, in solid phase chemical synthesis, the product harvesting method is mainly based on gas phase or liquid phase. For the liquid phase recovery method, the solid phase carrier is first placed in a reactor, and then a liquid deprotection reagent is introduced into the reactor to remove the protective group of the product on the solid phase carrier. After that, the liquid in the reactor is discharged, and a liquid linker cleavage reagent is introduced into the reactor to break the linker of the product on the solid phase carrier. After that, a buffer solution is introduced to elute the solid phase carrier, etc., to achieve product recovery.

[0004] However, when recovering the product, the liquid phase deprotection reagent or linker arm cleavage reagent, etc., is introduced into the reactor and remains stationary, resulting in low reaction efficiency and low product recovery efficiency. Utility Model Content

[0005] The embodiment of the present disclosure provides a reaction device for recovering products after solid phase chemical synthesis, which can improve the efficiency of recovering products after solid phase chemical synthesis. The technical solution is as follows:

[0006] An embodiment of the present disclosure provides a reaction device for recovering products after solid-phase chemical synthesis, wherein the reaction device includes a reaction component and a drive component, wherein the reaction component includes a reactor filled with reaction reagents and a temperature control unit for heating the reaction reagents in the reactor, wherein the temperature control unit is connected to the reactor; the drive component is located outside the reactor and connected to the reactor, and the drive component is used to drive the flow of liquid in the reactor so that the liquid can repeatedly flush the solid phase carrier in the reactor.

[0007] In another implementation of the present disclosure, the driving assembly includes a driving pump and a connecting pipe, the liquid inlet of the driving pump is connected to the reactor, and the liquid outlet of the driving pump is connected to the connecting pipe.

[0008] In yet another implementation of the present disclosure, the driving pump is a peristaltic pump.

[0009] In another implementation of the present disclosure, the reaction device further includes a monitoring component, which is located outside the reactor and on one side of the connecting pipe, and is used to monitor at least one performance parameter of the liquid in the connecting pipe.

[0010] In another implementation of the present disclosure, the connecting pipe includes a transparent pipe section; the monitoring component includes an ultraviolet light source and a monitor, the ultraviolet light source and the monitor are respectively located on both sides of the connecting pipe, and the ultraviolet light source and the monitor are both directly opposite to the transparent pipe section; the monitor is used to monitor the ultraviolet absorbance of the liquid in the connecting pipe.

[0011] In yet another implementation of the present disclosure, the monitor is an ultraviolet light sensor.

[0012] In another implementation of the present disclosure, the reaction device further includes a recovery container, and the recovery container is connected to an end of the communicating pipe away from the driving pump.

[0013] In another implementation of the present disclosure, the reaction device further includes a display unit, which is electrically connected to the monitor, and the display unit is used to display the monitoring results monitored by the monitor in real time.

[0014] In another implementation of the present disclosure, the reactor includes a container body and a sealing cover, wherein the sealing cover is rotatably located on the top of the container body and connected to the container body.

[0015] In another implementation of the present disclosure, the sealing cover is provided with a digitally controlled air valve.

[0016] The technical solution provided by the embodiments of the present disclosure brings the following beneficial effects:

[0017] Since the reaction device also includes a driving component, and the driving component is connected to the reactor, the driving component is used to drive the flow of liquid in the reactor so that the liquid can repeatedly flush the solid phase carrier in the reactor. In this way, the reaction reagents in the reactor can flow continuously on the surface of the solid phase carrier, and then the products in the solid phase carrier can fully contact with the reagents in the reactor, thereby improving the reaction rate and efficiency, thereby improving the recovery efficiency of the product. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 A schematic structural diagram of a reaction device provided in an embodiment of the present disclosure.

[0020] The symbols in the figure mean the following:

[0021] 1. Reaction assembly; 11. Reactor; 12. Temperature control unit; 111. Container body; 112. Sealing cover; 113. CNC air valve;

[0022] 2. driving assembly; 21. driving pump; 22. connecting pipe;

[0023] 3. Monitoring component; 31. Ultraviolet light source; 311. Ultraviolet lamp; 312. Power supply; 33. Monitor;

[0024] 4. Recovery container; 41. Cover cap; 42. Tube body;

[0025] 5. CNC and display unit. DETAILED DESCRIPTION

[0026] In order to make the objectives, technical solutions and advantages of the present disclosure more clear, the embodiments of the present disclosure will be further described in detail below with reference to the accompanying drawings.

[0027] During solid phase chemical synthesis, the product is usually anchored to a solid phase carrier through a connecting arm, and the product anchored to a solid phase carrier often has a protecting group. Therefore, before the product is recovered, it needs to be deprotected and the connecting arm broken. Among them, deprotection refers to removing the protecting group with the corresponding deprotecting group reagent. Breaking the connecting arm refers to cutting the connecting arm with the corresponding connecting arm cutting reagent. For example, in nucleic acid synthesis, a reagent (ammonia gas or ammonia water) can be used to deprotect the product and break the connecting arm. After deprotection and breaking the connecting arm, the product can be eluted and recovered, and the solid phase carrier can be discarded.

[0028] When recovering the product, there are generally liquid phase and gas phase methods. The so-called gas phase is to place the solid phase carrier in a reactor. Then the vaporized deprotection reagent is introduced to the surface of the solid phase carrier to remove the protecting group of the product on the solid phase carrier. After that, the vaporized linker cleavage reagent is introduced to the surface of the solid phase carrier to break the linker of the product on the solid phase carrier, so as to separate the product from the solid phase carrier. After that, the product is eluted and recovered, and the solid phase carrier is discarded.

[0029] The embodiments of the present disclosure provide a reaction device for recovering products after solid phase chemical synthesis, and the reaction device can be used for deprotection, cleavage of connecting arms, concentration, elution and other treatments when recovering the products.

[0030] like Figure 1 As shown, the reaction device comprises a reaction component 1 and a driving component 2. The reaction component 1 comprises a reactor 11 filled with a reaction reagent and a temperature control unit 12 for heating the reaction reagent in the reactor 11, and the temperature control unit 12 is connected to the reactor 11.

[0031] The driving component 2 is located outside the reactor 11 and connected to the reactor 11 . The driving component 2 is used to drive the liquid in the reactor 11 to flow, so that the liquid can repeatedly flush the solid phase carrier in the reactor 11 .

[0032] When the fixed synthetic product is recovered by providing multiple reaction devices through the embodiment of the present disclosure, since the reaction device includes a reactor 11 and a temperature control unit 12, the reaction reagent can be placed in the reactor 11, and then the solid phase carrier containing the product can be placed in the reactor 11, so that the solid phase carrier can contact the reaction reagent, and the product can be deprotected and the connecting arm can be broken. At the same time, the temperature in the reactor 11 can be controlled by the temperature control unit 12, so that the temperature in the reactor 11 can meet the required temperature.

[0033] Furthermore, since the reaction device also includes a driving component 2, and the driving component 2 is connected to the reactor 11, the driving component 2 is used to drive the flow of liquid in the reactor 11, so that the liquid can repeatedly flush the solid phase carrier in the reactor 11, so that the reaction reagents in the reactor can flow continuously on the surface of the solid phase carrier, and then the product in the solid phase carrier can fully contact with the reagent in the reactor 11, thereby improving the reaction rate and efficiency, thereby improving the recovery efficiency of the product.

[0034] In this embodiment, the reaction reagent in the reactor 11 can be a deprotection group reagent used for deprotection reaction of the product after solid phase chemical synthesis, or a linker cutting reagent used for breaking the linker of the product after solid phase chemical synthesis, or a buffer during elution, etc.

[0035] Optionally, the reactor 11 includes a container body 111 and a sealing cover 112, wherein the sealing cover 112 is rotatably located on the top of the container body 111 and connected to the container body 111. The sealing cover 112 is used to seal the container body 111, so that the reactor 11 can form a closed reaction space.

[0036] Optionally, the sealing cover 112 is provided with a digitally controlled gas valve 113. The digitally controlled gas valve is used to enable the reactor 11 to be vented, so that when a reaction such as product concentration is performed, the gas can be discharged through the digitally controlled gas valve.

[0037] Illustratively, the container body 111 may be any heatable reaction container, such as a reaction kettle, a heating tank, a flask, and the like.

[0038] In this embodiment, in order to facilitate the fixing of the solid phase carrier in the reactor 11, a rectangular reagent tank is provided inside the container body 111. The solid phase carrier is inserted into the rectangular reagent tank.

[0039] Exemplarily, the temperature control unit 12 may be one of an electric heating jacket, a constant temperature metal bath, and the like. In the present embodiment, the temperature control unit 12 is a constant temperature metal bath. Since the constant temperature metal bath is temperature controlled by a PID (Proportiona IntegralDifferential) temperature control system, the heating temperature of the reactor 11 can be controlled more accurately. As long as the reactor 11 can be automatically heated so that the reactor 11 can be in a suitable temperature environment, the present disclosure does not limit this.

[0040] Continue to see Figure 1 Optionally, the driving assembly 2 includes a driving pump 21 and a connecting pipe 22, the liquid inlet of the driving pump 21 is connected to the reactor 11, and the liquid outlet of the driving pump 21 is connected to the connecting pipe 22, and the driving pump 21 is used to control the liquid in the reactor 11 to flow between the connecting pipe 22 and the reactor 11.

[0041] In the above implementation, the driving pump 21 provides power for the liquid in the reactor 11, so that the liquid can flow quickly, thereby accelerating the diffusion rate of the liquid, so that the liquid in the reactor 11 can fully contact with the solid phase carrier. The connecting pipe 22 is used to further provide space for the liquid in the reactor 11, so that the liquid can flow between the reactor 11 and the connecting pipe 22.

[0042] Of course, the driving assembly 2 can also be other structural forms, such as a stirring device, but the stirring device is easy to interfere with the solid phase carrier after being placed in the reactor 11, and is inconvenient to arrange.

[0043] In addition, in the present application, the connecting pipe 22 is provided because the liquid in the connecting pipe 22 can be monitored by the monitoring component 3 (described below) to further confirm whether the reaction in the reactor 11 is completed or whether the liquid in the connecting pipe 22 overflows. Moreover, after the reaction in the reactor 11 is completed, the liquid in the reactor 11 can also be directly recovered through the connecting pipe 22, which is convenient for the recovery of the liquid.

[0044] Exemplarily, the driving pump 21 is a peristaltic pump, which is easy to operate, has low noise and low maintenance cost. The driving pump 21 has a positive rotation mode and a negative rotation mode. When the driving pump 21 is in the positive rotation mode, it can drive the liquid in the reactor 11 to flow into the connecting pipe 22. When the driving pump 21 is in the negative rotation mode, it can drive the liquid in the connecting pipe 22 to flow back into the reactor 11.

[0045] In this embodiment, when relevant operations are performed in the reactor 11, the driving pump 21 is controlled to be in different working modes and different rotation speeds, so that the liquid in the reactor 11 flows back and forth between the reactor 11 and the connecting pipe 22 and does not overflow from the connecting pipe 22.

[0046] Optionally, the reaction device further includes a monitoring component 3 , which is located outside the reactor 11 and on one side of the connecting pipe 22 , and is used to monitor at least one performance parameter of the liquid in the connecting pipe 22 .

[0047] The monitoring component 3 is used to monitor at least one performance parameter of the liquid in the connecting pipe 22 in real time, so that the liquid in the connecting pipe 22 can be prevented from overflowing according to the monitored performance parameter. Alternatively, it can be identified whether the relevant reaction in the reactor 11 is completed according to the performance parameter.

[0048] That is, after the solid phase carrier is inserted into the flow groove in the reactor 11, the liquid in the reactor 11 can be made to flow back and forth between the reactor 11 and the connecting pipe 22 by controlling the driving pump 21. At the same time, through the monitoring of the monitoring component 3, it can be ensured that the liquid will not overflow from the connecting pipe 22. At the same time, according to the performance parameters monitored by the monitoring component 3, it can also be confirmed whether the reaction in the reactor 11 is completed.

[0049] One performance parameter may be ultraviolet absorbance.

[0050] Exemplarily, the monitoring assembly 3 is arranged within the limit of the liquid overflowing from the communication pipe 22 .

[0051] Optionally, the communication pipe 22 includes a transparent pipe section.

[0052] The monitoring assembly 3 includes an ultraviolet light source 31 and a monitor 33, which are respectively located on both sides of the connecting pipe 22, and both are directly opposite to the transparent pipe section. The monitor 33 is used to monitor the ultraviolet absorbance of the liquid in the connecting pipe 22.

[0053] Different substances have different light absorption properties. For example, DNA prefers to absorb 260nm ultraviolet light, protein prefers to absorb 280nm ultraviolet light, chloroform prefers to absorb 245nm ultraviolet light, toluene prefers to absorb 300nm, etc. Therefore, it is possible to judge whether the corresponding product obtained in the reaction carried out in the reactor 11 reaches a peak value based on the ultraviolet absorbance of different substances to determine whether the reaction is completed.

[0054] When the communicating pipe 22 is filled with air, since the air is almost completely transparent (almost does not absorb ultraviolet light), the ultraviolet light emitted by the ultraviolet light source 31 can almost all pass through the transparent pipe section of the communicating pipe 22 to reach the monitor 33. At this time, the ultraviolet absorbance monitored in the monitor 33 is the minimum value.

[0055] When the liquid in the reactor 11 flows into the connecting pipe 22 , the liquid in the connecting pipe 22 absorbs part of the ultraviolet light, so the ultraviolet absorbance monitored in the monitor 33 gradually increases.

[0056] When there is a target product in the connecting pipe 22, the target product absorbs ultraviolet light of the corresponding characteristic wavelength, and the ultraviolet absorbance monitored in the monitor 33 at the characteristic wavelength gradually increases, and when it reaches the maximum, it is determined that the reaction is finished.

[0057] The ultraviolet light source 31 and the monitor 33 mentioned above are both directly opposite to the transparent pipe section, which means that along the axis direction perpendicular to the transparent pipe section, the projection of the ultraviolet light source 31 on the transparent pipe section is exactly located on the transparent pipe section. The projection of the monitor 33 on the transparent pipe section is also exactly located on the transparent pipe section.

[0058] Exemplarily, the communication pipe 22 is a transparent L-shaped curved pipe structure. The communication pipe 22 includes a horizontal section and a vertical section perpendicular to each other. In this way, the communication pipe 22 can be connected to the driving pump 21 through the horizontal section, so that the liquid in the communication pipe 22 will not easily overflow. At the same time, the vertical section of the communication pipe 22 is connected to the recovery container 4, so that the liquid in the communication pipe 22 can be quickly recovered to the recovery container 4 by gravity.

[0059] Optionally, the monitor 33 is an ultraviolet light sensor with a driver chip.

[0060] In the above implementation, the monitor 33 is configured as an ultraviolet light sensor with a driving chip, so that the ultraviolet absorbance of the liquid in the communicating pipe 22 can be directly obtained.

[0061] Optionally, the ultraviolet light source 31 includes an ultraviolet lamp 311 and a power supply 312 , and the ultraviolet lamp 311 is electrically connected to the positive and negative electrodes of the power supply 312 .

[0062] The ultraviolet lamp 311 is used to emit ultraviolet light of different wavelengths. The power supply 312 is used to provide power to the ultraviolet lamp 311. Of course, the ultraviolet light source 31 can also be other structural forms, such as an ultraviolet lamp with a power storage device, etc.

[0063] Exemplarily, the ultraviolet lamp 311 may be an LED (Light Emitting Diode) light source capable of emitting ultraviolet light.

[0064] Optionally, the reaction device further comprises a recovery container 4 , which is connected to an end of the communication pipe 22 away from the driving pump 21 .

[0065] In the above implementation, the recovery container 4 is used to recover the liquid in the reactor 11 under the drive of the driving pump 21 after the reaction in the reactor 11 is completed.

[0066] In this embodiment, the recovery container 4 is a 50 ml plastic centrifuge tube. The recovery container 4 includes a cap 41 and a tube body 42. The cap 41 is sealed at the opening of the tube body 42 and is clamped together with the outer wall of the tube body 42. The middle part of the cap 41 has a threaded hole, and the connecting pipe 22 is sealed and inserted into the threaded hole and is threadedly connected with the threaded hole.

[0067] Of course, the above recovery container 4 can also be other devices, such as glass bottles, etc. It is just that the cost of plastic centrifuge tubes is lower.

[0068] Optionally, the reaction device further comprises a numerical control and display unit 5 , which is electrically connected to the monitor 33 , and is used for displaying the monitoring results monitored by the monitor 33 in real time.

[0069] In the above implementation, the numerical control and display unit 5 can display the monitoring results monitored by the monitor 33 in real time, so that the staff can understand the monitoring results monitored by the monitor 33 in real time.

[0070] In this embodiment, the numerical control and display unit 5 can be a computer, a mobile phone, etc.

[0071] Exemplarily, the numerical control and display unit 5 is electrically connected to the temperature control unit 12 , and is used to control the working parameters of the temperature control unit 12 , and can also display the heating temperature in the reactor 11 .

[0072] In this embodiment, the numerical control and display unit 5 is electrically connected to the driving pump 21, and is used to automatically control the driving pump 21. For example, the start and stop, speed and direction of the driving pump 21 are automatically controlled.

[0073] The following briefly introduces the working process of the reaction device provided in the embodiment of the present disclosure:

[0074] (1) When the deprotection or washing process is carried out in the reactor 11:

[0075] When the product is anchored in a solid phase support, the protecting groups can be removed using a deprotection reagent, or impurities can be removed by washing with a buffer solution.

[0076] A solid phase carrier is inserted into the reactor 11, and the recovery container 4 is connected to the connecting pipe 22. The heating temperature of the temperature control unit 12 is set, and a deprotection reagent or a buffer solution is added to the reactor 11. When the temperature of the liquid in the reactor 11 does not reach the set temperature, the driving pump 21 will not be turned on. The connecting pipe 22 is basically air. When the liquid in the reactor 11 is at a set constant temperature, the driving pump 21 turns on the forward mode, and the liquid fills the connecting pipe 22. The driving pump 21 rotates alternately in the positive and negative directions according to the set parameters (the parameters of the driving pump 21 are set, combined with the readings of the monitor 33, so that the liquid in the reactor 11 flows through the transparent pipe section and reaches the maximum limit position of the connecting pipe 22 without overflowing, and the driving pump 21 just reverses, so that the liquid flows back to the reactor 11), so that the liquid swings back and forth in the reactor 11 and the connecting pipe 22 to prevent the liquid from overflowing from the connecting pipe 22. After the reaction is finished, the driving pump 21 starts the fast forward mode to drive all the liquids in the connecting pipe 22 and the reactor 11 to flow into the recovery container 4 .

[0077] (2) When the linker cleavage reaction is performed in the reactor 11:

[0078] The purpose of the linker cleavage reaction is to cut off the linker and ultimately recover the product.

[0079] A solid phase carrier is inserted into the reactor 11, and the recovery container 4 is connected to the connecting pipe 22. The heating temperature of the temperature control unit 12 is set, and the connecting arm cutting reagent is added to the reactor 11. When the temperature of the liquid in the reactor 11 does not reach the set temperature, the driving pump 21 will not be turned on. The connecting pipe 22 is basically air. When the liquid in the reactor 11 is at a set constant temperature, the driving pump 21 turns on the forward mode, and the liquid fills the connecting pipe 22. The driving pump 21 rotates alternately in the positive and negative directions according to the set parameters, so that the liquid swings back and forth in the reactor 11 and the connecting pipe 22 to prevent the liquid from overflowing from the connecting pipe 22. At the same time, the precipitation concentration of the target product is monitored by the monitor 33. When the concentration of the target product enters the plateau phase, the reaction is determined to be over. Or the maximum time set by the user (if set) is reached. The driving pump 21 turns on the fast forward mode, driving the liquid in the connecting pipe 22 and the reactor 11 to flow to the recovery container 4. If it is necessary to continue the concentration reaction later, the liquid in the reactor 11 does not need to be recovered to the recovery container 4.

[0080] (3) When the reactor 11 is performing concentration and volatile matter removal operations:

[0081] This process is to reduce the concentration of water or volatiles in the reactor 11. This step is generally followed by "deprotection and washing" or "linker cleavage".

[0082] The recovery container 4 is connected to the connecting pipe 22. The heating temperature of the temperature control unit 12 is set. When the temperature of the liquid in the reactor 11 does not reach the set temperature, the driving pump 21 will not be turned on. The connecting pipe 22 is basically air. When the liquid in the reactor 11 is at a set constant temperature, the driving pump 21 turns on the forward mode, and the liquid fills the connecting pipe 22. The driving pump 21 rotates alternately in the positive and negative directions according to the set parameters, so that the liquid swings back and forth in the solid phase carrier and the connecting pipe 22 to prevent the liquid from overflowing from the connecting pipe 22. At the same time, the concentration of the target product is monitored by the monitor 33. When the concentration of the target product enters the plateau phase, the reaction is determined to be over. Or the maximum time set by the user (if set) is reached. The driving pump 21 turns on the fast forward mode, driving the liquid in the connecting pipe 22 and the reactor 11 to flow to the recovery container 4 to recover the product.

[0083] The above are only optional embodiments of the present disclosure and are not intended to limit the present disclosure. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present disclosure should be included in the protection scope of the present disclosure.

Claims

1. A reaction device for recovering products after solid phase chemical synthesis, characterized in that: The reaction device comprises a reaction component (1) and a driving component (2). The reaction assembly (1) comprises a reactor (11) containing a reaction reagent and a temperature control unit (12) for heating the reaction reagent in the reactor (11), wherein the temperature control unit (12) is connected to the reactor (11); The driving component (2) is located outside the reactor (11) and is connected to the reactor (11). The driving component (2) is used to drive the flow of liquid in the reactor (11) so that the liquid can repeatedly flush the solid phase carrier in the reactor (11).

2. The reaction device according to claim 1, characterized in that: The driving assembly (2) comprises a driving pump (21) and a connecting pipe (22), wherein the liquid inlet of the driving pump (21) is connected to the reactor (11), and the liquid outlet of the driving pump (21) is connected to the connecting pipe (22).

3. The reaction device according to claim 2, characterized in that: The driving pump (21) is a peristaltic pump.

4. The reaction device according to claim 2, characterized in that: The reaction device further comprises a monitoring component (3), wherein the monitoring component (3) is located outside the reactor (11) and on one side of the connecting pipe (22), and the monitoring component (3) is used to monitor at least one performance parameter of the liquid in the connecting pipe (22).

5. The reaction device according to claim 4, characterized in that: The communicating pipe (22) comprises a transparent pipe section; The monitoring component (3) comprises an ultraviolet light source (31) and a monitor (33), wherein the ultraviolet light source (31) and the monitor (33) are respectively located on two sides of the connecting pipe (22), and both the ultraviolet light source (31) and the monitor (33) are directly opposite to the transparent pipe section; The monitor (33) is used to monitor the ultraviolet absorbance of the liquid in the connecting pipe (22).

6. The reaction device according to claim 5, characterized in that: The monitor (33) is an ultraviolet light sensor.

7. The reaction device according to claim 2, characterized in that: The reaction device further comprises a recovery container (4), wherein the recovery container (4) is connected to an end of the communication pipe (22) away from the driving pump (21).

8. The reaction device according to claim 5, characterized in that: The reaction device further comprises a numerical control and display unit (5), wherein the numerical control and display unit (5) is electrically connected to the monitor (33), and the numerical control and display unit (5) is used to display the monitoring results monitored by the monitor (33) in real time.

9. The reaction device according to any one of claims 1 to 8, characterized in that: The reactor (11) comprises a container body (111) and a sealing cover (112). The sealing cover (112) is rotatably located on the top of the container body (111) and is connected to the container body (111).

10. The reaction device according to claim 9, characterized in that: The sealing cover (112) is provided with a digitally controlled air valve (113).

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