Shaking table proliferation device and phage display device with same

Abstract

The utility model provides a shaker proliferation device and phage display device with the shaker proliferation device, the shaker proliferation device comprises: an oscillation module comprising a culture bottle placing unit and an oscillation driving structure used for driving the culture bottle placing unit to oscillate; the transverse moving module comprises a transverse moving driving part and a shaking table support used for fixing the transverse moving driving part, a transverse moving sliding rail assembly is arranged between the shaking table support and the oscillation module, and the transverse moving driving part can drive the oscillation module to slide in the guiding direction of the transverse moving sliding rail assembly. The position of the shaking table proliferation device has displacement capacity, so that the shaking table proliferation device can be aligned with a corresponding liquid suction needle after being applied to a corresponding bacteriophage device, the requirement for the degree of freedom of motion of the liquid suction needle can be lowered, and the design difficulty of a motion driving mechanism of the liquid suction needle can be lowered.

Description

Technical Field

[0001] The utility model belongs to the technical field of phage display equipment design, and in particular relates to a shaking table proliferation device and a phage display device having the same. Background Art

[0002] Phage is a type of tiny life form that infects and replicates inside bacteria. Using synthetic biology tools, the genetic information of phage can be reprogrammed, making phage or infected bacteria an important tool for drug screening, protein optimization, and biomarker screening.

[0003] Phage display technology is one of the two major development platforms for all approved antibody drugs in the world. Due to its mature research system, low cost, and simple operation, phage display technology has attracted widespread attention from researchers and has extremely important commercial value.

[0004] In the field of antibody preparation, phage display technology provides a simple and fast genetic engineering antibody preparation route for fully human recombinant antibodies: a complete set of antibody heavy chain variable region (VH) and light chain variable region (VL) genes are amplified from human immune cells using PCR technology and expressed on the phage surface in the form of fusion proteins.

[0005] Various types of antibodies can be screened through phage display technology. Compared with monoclonal antibodies produced by immunizing animals, monoclonal antibodies produced using this technology have the advantages of higher efficiency and faster production.

[0006] The current phage display technology is implemented by a combination of manual operations of equipment such as full-temperature vibration incubators, centrifuges, and clone panning equipment. The aforementioned equipment is a single-function and independent device, and requires operator participation, which leads to the risk of cross-contamination during the culture and panning process. The manual participation also leads to manual selection deviations, reducing the stability of the process and results. In addition, the independent application of single-function modules and the low degree of equipment integration lead to time-consuming and high-cost phage screening and display. It should be noted that the shaker proliferation device in the prior art is an independent device and does not have the function of aligning the culture bottle. Therefore, it cannot be used in an automated phage device. Based on the above-mentioned deficiencies in the prior art, the utility model proposes a shaker proliferation device used in the phage display device. Utility Model Content

[0007] Therefore, the technical problem to be solved by the utility model is to provide a shaking table proliferation device and a phage display device having the same, so as to solve the technical problem that the shaking table proliferation device in the prior art cannot move horizontally to achieve the alignment of the culture bottle and the corresponding aspiration needle thereon, and is therefore not suitable for use in an automated phage device.

[0008] In order to solve the above problems, the utility model provides a shaking table proliferation device, comprising:

[0009] An oscillation module, comprising a culture bottle placement unit and an oscillation driving structure for driving the culture bottle placement unit to oscillate;

[0010] The transverse movement module includes a transverse movement driving component and a rocking table bracket for fixing the transverse movement driving component. A transverse movement slide rail assembly is provided between the rocking table bracket and the oscillation module. The transverse movement driving component can drive the oscillation module to slide along the guiding direction of the transverse movement slide rail assembly.

[0011] In some embodiments, the transverse movement driving component is a stepper motor, and the transverse movement module also includes an idler wheel and a transmission belt assembled on the rocking table bracket, the transmission belt is tensioned between the idler wheel and the output shaft of the stepper motor, and the transmission belt is fixedly connected to the oscillation module.

[0012] In some embodiments,

[0013] The oscillation driving structure includes an oscillation base plate, the bottom side of the oscillation base plate is connected to the rocking table bracket through the transverse slide rail assembly, and the oscillation base plate is also provided with a connecting fixed arm, the bottom end of the connecting fixed arm is fixedly connected to the transmission belt.

[0014] In some embodiments,

[0015] The oscillation drive structure also includes a bearing support located in the top side area of ​​the oscillation base plate, an oscillation drive motor is assembled on the oscillation base plate, an eccentric cam is mounted on the output shaft of the oscillation drive motor, and the eccentric cam is rotatably located in a bearing chamber on the bottom side of the bearing support, the culture bottle placement unit includes a placement base plate, the bearing support is arranged on the bottom side of the placement base plate, and the oscillation drive structure also includes a plurality of elastic members, which are connected between the placement base plate and the oscillation base plate to apply elastic force along its radial direction to the placement base plate.

[0016] In some embodiments, a bearing is disposed between the eccentric cam and the bearing support.

[0017] In some embodiments, a positioning assembly is provided between the oscillation drive structure and the rocking table bracket, and the positioning assembly includes a photoelectric baffle fixed to the connecting and fixing arm and a photoelectric sensor located on the rocking table bracket.

[0018] In some embodiments, the elastic member is a tension spring, and both ends of the tension spring are connected to the placement base plate and the oscillation base plate respectively through fixing pins.

[0019] In some embodiments, an outer cover is provided on the top side of the placement bottom plate, and a plurality of placement holes are formed on the outer cover, and each of the placement holes is used to place a culture bottle.

[0020] In some embodiments, a heating film is further provided on the top side of the placement base plate to heat the culture bottle placed in the placement hole; and / or, an elastic fixing ring is provided in each placement hole to fix the position of the culture bottle placed in the placement hole.

[0021] The present invention also provides a phage display device, comprising the shaking table proliferation device.

[0022] The utility model provides a shaking table proliferation device and a phage display device having the same. The transverse movement module realizes sliding drive of the oscillation module through the transverse movement driving component, so that the position of the shaking table proliferation device in the utility model has displacement capability, and then the shaking table proliferation device can be applied to the corresponding phage device and then aligned with the corresponding liquid pipetting needle. This can also reduce the demand for the movement freedom of the liquid pipetting needle and reduce the design difficulty of the movement driving mechanism of the liquid pipetting needle. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the three-dimensional structure of the shaking table proliferation device of the embodiment of the utility model;

[0024] Figure 2 for Figure 1 Side view of

[0025] Figure 3 for Figure 1 Top view of the medium oscillation drive structure;

[0026] Figure 4 for Figure 3 Cross-section of middle JJ;

[0027] Figure 5 for Figure 2 Bottom view of .

[0028] The reference numerals are:

[0029] 21. Oscillation module; 211. Oscillation base plate; 2111. Connecting fixed arm; 212. Bearing support; 213. Oscillation drive motor; 214. Eccentric cam; 215. Elastic member; 216. Bearing; 2171. Photoelectric baffle; 2172. Photoelectric sensor; 218. Fixing pin; 22. Transverse movement module; 221. Transverse movement drive component; 222. Shaking table bracket; 223. Transverse movement slide rail assembly; 224. Idle wheel; 225. Transmission belt; 231. Placement base plate; 232. Outer cover; 233. Elastic fixing ring; 24. Controller; 100. Culture bottle. DETAILED DESCRIPTION

[0030] See also Figures 1 to 5 As shown, according to an embodiment of the utility model, a shaking table proliferation device is provided, comprising:

[0031] The oscillation module 21 includes a culture bottle placement unit (not labeled in the figure) and an oscillation driving structure (not labeled in the figure) for driving the culture bottle placement unit to oscillate;

[0032] The transverse movement module 22 includes a transverse movement driving component 221 and a rocking table bracket 222 for fixing the transverse movement driving component 221. A transverse movement slide rail assembly 223 is provided between the rocking table bracket 222 and the oscillation module 21. The transverse movement driving component 221 can drive the oscillation module 21 to slide along the guiding direction of the transverse movement slide rail assembly 223. The transverse movement slide rail assembly 223 specifically includes a guide rail fixedly connected to the rocking table bracket 222 and a slider fixedly connected to the oscillation module 21, and the slider is slidably connected to the guide rail.

[0033] In this technical solution, the transverse movement module 22 realizes the sliding drive of the oscillation module 21 through the transverse movement driving component 221, so that the position of the rocking table proliferation device in the utility model has the ability to move, and then the rocking table proliferation device can be applied to the corresponding phage device and aligned with the corresponding pipetting needle. This can also reduce the demand for the freedom of movement of the pipetting needle and reduce the difficulty of designing the movement driving mechanism of the pipetting needle.

[0034] In some embodiments, the transverse driving component 221 is a stepper motor, and the transverse module 22 also includes an idler wheel 224 and a transmission belt 225 assembled on the rocking bed bracket 222. The transmission belt 225 is tensioned between the idler wheel 224 and the output shaft of the stepper motor, and the transmission belt 225 is fixedly connected to the oscillation module 21. See the figure for details. The transmission belt 225 can also be called a synchronous belt, which is wrapped around the output shaft of the stepper motor and the relative outer sides of the idler wheel 224 to form a wrap angle, and at the same time realizes the transmission tensioning and installation of the transmission belt 225. It can be understood that the reciprocating motion direction of the transmission belt 225 should be parallel to the guiding direction and sliding direction of the aforementioned transverse slide rail assembly 223, so that the displacement drive structure design of the oscillation module 21 can be simplified, and the manufacturing cost is relatively low. In a specific embodiment, the oscillation drive structure includes an oscillation base plate 211, the bottom side of the oscillation base plate 211 is connected to the rocking table bracket 222 through the transverse slide rail assembly 223, and the oscillation base plate 211 also has a connecting fixed arm 2111, and the bottom end of the connecting fixed arm 2111 is fixedly connected to the transmission belt 225, see Figure 4As shown, the connecting and fixing arm 2111 is a T-shaped arm structure, and the width of the lower end thereof is relatively large, and can be relatively buckled with the corresponding buckle plate to achieve a reliable and stable connection with the transmission belt 225. The top end of the connecting and fixing arm 2111 is detachably connected to the bottom side of the oscillating bottom plate 211 by bolts.

[0035] See also Figure 4 As shown, the oscillation drive structure also includes a bearing support 212 located in the top area of ​​the oscillation bottom plate 211, an oscillation drive motor 213 is assembled on the oscillation bottom plate 211, an eccentric cam 214 is mounted on the output shaft of the oscillation drive motor 213, and the eccentric cam 214 is rotatably located in a bearing chamber (not marked in the figure) on the bottom side of the bearing support 212. The culture bottle placement unit includes a placement bottom plate 231 (see Figure 2 As shown in the figure, the bearing support 212 is arranged on the bottom side of the placement base plate 231, and the oscillation drive structure also includes a plurality of elastic members 215, which are connected between the placement base plate 231 and the oscillation base plate 211 to apply elastic force to the placement base plate 231 along its radial direction. The plurality of elastic members 215 can rely on their elastic force to prevent the culture bottle placement unit from rotating around the aforementioned eccentric cam 214, and can also work together with the eccentric cam 214 to act on the culture bottle placement unit so that it can oscillate in translation, thereby improving the oscillation effect. In a specific embodiment, the aforementioned elastic member 215 is a tension spring, and the two ends of the tension spring are respectively connected to the placement base plate 231 and the oscillation base plate 211 through a fixing pin 218, which has a simple structure and low manufacturing cost.

[0036] In some embodiments, a bearing 216 is provided between the eccentric cam 214 and the bearing support 212. The bearing 216 may be, for example, a ball bearing. By arranging the bearing 216 between the eccentric cam 214 and the bearing support 212, the oscillation smoothness of the culture bottle placement unit can be improved and the wear of the eccentric cam 214 can be reduced.

[0037] In some embodiments, a positioning assembly is provided between the oscillation drive structure and the rocking table bracket 222, and the positioning assembly includes a photoelectric baffle 2171 fixed to the connecting fixed arm 2111 and a photoelectric sensor 2172 located on the rocking table bracket 222, see Figure 5 As shown, the positioning component includes a photoelectric baffle 2171 fixed to the connecting fixed arm 2111 and a photoelectric sensor 2172 located on the rocking table bracket 222. The photoelectric sensor 2172 can be a photoelectric sensor on the market with detection accuracy that meets the requirements. The positioning component can accurately determine the specific position of the oscillation drive structure, thereby ensuring the accuracy of the position adjustment of the culture bottle 100 in the culture bottle placement unit.

[0038] See also Figure 1 and Figure 2 As shown, in some embodiments, an outer cover 232 is provided on the top side of the placement bottom plate 231, and the outer cover 232 can be bolted to the top surface of the placement bottom plate 231. A plurality of placement holes (not labeled in the figure) are formed on the outer cover 232, and each of the placement holes is used to place a culture bottle 100, so that the purpose of shaking multiple culture bottles 100 at a time can be achieved, thereby improving the proliferation flux. The apertures of the aforementioned plurality of placement holes can have multiple sizes.

[0039] In another preferred embodiment, a heating film (not shown and not labeled) is further provided on the top side of the placement base plate 231 so as to heat the culture bottle 100 placed in the placement hole, thereby meeting the incubation and proliferation temperature requirements of different samples. The aforementioned heating film is, for example, an electric heating film (graphene film, etc.).

[0040] In other embodiments, in order to ensure that each culture bottle 100 does not have a rigid collision with the outer cover 232 during the shaking process of the shaking table, an elastic fixing ring 233 is provided in each placement hole to fix the position of the culture bottle 100 placed in the placement hole. In a specific embodiment, the aforementioned elastic fixing ring 233 has four elastic claws arranged in a cross shape, and each elastic claw jointly clamps and holds the culture bottle 100 placed inside thereof.

[0041] A controller 24 is also provided on the rocking table support 222 for controlling the operation of the aforementioned oscillation drive motor 213 and the transverse movement drive component 221 .

[0042] According to an embodiment of the present invention, there is also provided a phage display device, comprising the above-mentioned shaking table proliferation device.

[0043] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0044] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred implementations of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A shaking table proliferation device, characterized in that: include: An oscillation module (21), comprising a culture bottle placement unit and an oscillation driving structure for driving the culture bottle placement unit to oscillate; The transverse movement module (22) comprises a transverse movement driving component (221) and a rocking table bracket (222) for fixing the transverse movement driving component (221); a transverse movement slide rail assembly (223) is provided between the rocking table bracket (222) and the oscillation module (21); and the transverse movement driving component (221) can drive the oscillation module (21) to slide along the guiding direction of the transverse movement slide rail assembly (223).

2. The shaking table proliferation device according to claim 1, characterized in that: The transverse movement driving component (221) is a stepper motor, and the transverse movement module (22) further comprises an idler wheel (224) and a transmission belt (225) assembled on the rocking table bracket (222), wherein the transmission belt (225) is tensionedly connected between the idler wheel (224) and the output shaft of the stepper motor, and the transmission belt (225) is fixedly connected to the oscillation module (21).

3. The shaking table proliferation device according to claim 2, characterized in that: The oscillation drive structure comprises an oscillation base plate (211), the bottom side surface of the oscillation base plate (211) is connected to the rocking table bracket (222) via the transverse sliding rail assembly (223), and the oscillation base plate (211) is also provided with a connecting and fixing arm (2111), and the bottom end of the connecting and fixing arm (2111) is fixedly connected to the transmission belt (225).

4. The shaking table proliferation device according to claim 3, characterized in that: The oscillation drive structure also includes a bearing support (212) located in the top side area of ​​the oscillation bottom plate (211); an oscillation drive motor (213) is assembled on the oscillation bottom plate (211); an eccentric cam (214) is mounted on the output shaft of the oscillation drive motor (213); the eccentric cam (214) is rotatably located in a bearing chamber on the bottom side of the bearing support (212); the culture bottle placement unit includes a placement bottom plate (231); the bearing support (212) is arranged on the bottom side of the placement bottom plate (231); the oscillation drive structure also includes a plurality of elastic members (215); the plurality of elastic members (215) are connected between the placement bottom plate (231) and the oscillation bottom plate (211) to apply an elastic force along the radial direction to the placement bottom plate (231).

5. The shaking table proliferation device according to claim 4, characterized in that: A bearing (216) is disposed between the eccentric cam (214) and the bearing support (212).

6. The shaking table proliferation device according to claim 4, characterized in that: A positioning assembly is provided between the oscillation drive structure and the rocking bed bracket (222), and the positioning assembly comprises a photoelectric baffle (2171) fixed to the connecting and fixing arm (2111) and a photoelectric sensor (2172) located on the rocking bed bracket (222).

7. The shaking table proliferation device according to claim 4, characterized in that: The elastic member (215) is a tension spring, and the two ends of the tension spring are respectively connected to the placement base plate (231) and the oscillation base plate (211) through fixing pins (218).

8. The shaking table proliferation device according to claim 4, characterized in that: An outer cover (232) is provided on the top side of the placement bottom plate (231), and a plurality of placement holes are formed on the outer cover (232), each of the placement holes being used to place a culture bottle (100).

9. The shaking table proliferation device according to claim 8, characterized in that: A heating film is also provided on the top side of the placement bottom plate (231) so as to heat the culture bottle (100) placed in the placement hole; and / or, An elastic fixing ring (233) is provided in each placement hole for fixing the position of the culture bottle (100) placed in the placement hole.

10. A phage display device, characterized in that: A shaking table proliferation device comprising any one of claims 1 to 9.

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