Sleeve type centrifugal tube

Abstract

The utility model relates to the technical field of experiment detection centrifugal tubes, in particular to a sleeve type centrifugal tube which comprises an outer tube, an inner hose is arranged in the outer tube, and the outer tube and the inner hose are connected with each other through a fixing assembly; the inner hose comprises a hose body and a conical part, the hose body and the conical part are connected through a connecting assembly, and the connecting assembly is used for connecting the hose body and the conical part in a sealed mode. When the device is used, through the outer tube and the inner hose which are arranged in a nested mode, the inner hose can be directly taken out by clamping a hanging lug through tweezers after centrifugation is conducted, then a specimen in a conical part can be rapidly taken out after the conical part is taken down by damaging a connecting assembly, and the experiment steps are greatly simplified; compared with an existing centrifugal tube, the caliber of the bottom of the conical part is smaller, so that cell culture with a small cell quantity can be conveniently collected and obtained, the cell culture can be stored for a long time, and the quantity of cultured organoids can be reserved to the maximum extent.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental detection centrifuge tubes, in particular to a sleeve-type centrifuge tube. Background Art

[0002] Organoids are cell aggregates that are similar in structure and function to real organs, established using in vitro 3D culture technology. They have characteristics such as cell self-renewal, organ physiological activity, long-term culture, and genetic stability. Compared with traditional in vitro models, they are closer to the physiological state of organs in vivo and have great application prospects in the field of life science research. Because the genetic inheritance, cell proliferation, and cell differentiation of organoids are controlled by stem cell groups, the internal structure, phenotype, and cell types of organoids vary under the stimulation of drugs, cytokines, viruses, bacteria, etc., therefore, morphological and pathological histological testing of organoids is particularly important.

[0003] Paraffin sections in pathology are the most widely used method in routine histological preparation techniques. They can not only be used to observe the morphological structure of normal cell tissues, but are also the main method used by pathology and forensic medicine to study, observe and judge the morphological changes of cell tissues. They have also been widely used in research in other disciplines. Their advantages lie in accurate cell positioning and intact preservation of cell morphological structure. They can be stored for a long time at room temperature and can be used as permanent microscope slide specimens.

[0004] Existing pathological technology collects the organoids in a disposable centrifugal tube for centrifugation, collects the organoid sediment after centrifugation, and then fixes the organoids in the disposable centrifugal tube, wraps them with agarose, and finally cuts the disposable centrifugal tube to remove the solidified agar. After the agar is embedded, it is difficult to cut the cell surface for paraffin sections, and continuous sections can only be performed blindly during slicing, which not only wastes experimental time and increases experimental costs, but also makes it difficult to obtain structurally complete organoid sample slices, and even causes experimental failure. There are centrifugal tubes on the market with a large bottom diameter, which causes excessive loss in embedding and slicing of organoids with a small amount of cultured cells, making it difficult to proceed to the next step such as immunohistochemistry, special staining, in situ hybridization and other experiments. The new technology facilitates the collection of cells with a small amount of cells, can solve various problems in embedding and slicing, so that they can be preserved for a long time, and can retain the number of cultured organoids to the greatest extent, meeting the histological observation requirements of scientific research, which is a technical problem that this field has always been eager to solve. Utility Model Content

[0005] The purpose of the utility model is to provide a sleeve type centrifuge tube to solve the problems raised in the above background technology.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A sleeve type centrifuge tube, comprising:

[0008] An outer tube, wherein an inner hose is arranged inside the outer tube, and the outer tube and the inner hose are connected to each other via a fixing assembly;

[0009] The inner hose comprises a hose body and a tapered portion, wherein the hose body and the tapered portion are connected to each other via a connecting assembly, and the connecting assembly is used for sealingly connecting the hose body and the tapered portion.

[0010] Preferably, the outer tube is provided with a tube cover.

[0011] Preferably, the outer tube is provided with a fixing groove, and the fixing assembly includes a hanging ear, and the hanging ear is fixedly connected to the hose body, and the outer tube and the inner hose are relatively fixed by inserting the hanging ear into the fixing groove.

[0012] Preferably, the width of the fixing groove and the hanging ear are both 5 mm.

[0013] Preferably, the connecting assembly comprises an externally threaded tube, the externally threaded tube is arranged at the bottom of the hose body, the tapered portion is provided with an internal thread, and the hose body and the tapered portion are sealed and connected by screwing the tapered portion onto the externally threaded tube.

[0014] Preferably, the connecting assembly further comprises a connecting ring and connecting ridges, the connecting ring being arranged at the connection between the hose body and the tapered portion, the connecting ridges being arranged at equal intervals on the connecting ring and being used to connect the hose body and the tapered portion to each other.

[0015] Preferably, the tube diameter of the divergent end of the tapered portion and the tube diameter of the hose body are both 12 mm, the convergent end of the tapered portion is a round bottom structure with a diameter of 5 mm, and the length of the tapered portion is 8 mm.

[0016] Compared with the prior art, the utility model has the following beneficial effects: when the utility model is used, the outer tube and the inner tube are nested, and after centrifugation, the inner tube can be directly taken out by clamping the ear with tweezers, and then the conical part can be removed by artificially destroying the connection assembly (i.e., pulling the connecting ring and then rotating the conical part), and then the specimen in the conical part can be quickly taken out, which greatly simplifies the experimental steps and is very practical; the bottom diameter of the conical part in the present application is smaller than that of the existing centrifuge tube, which is convenient for collecting and obtaining cell culture with a small amount of cells, and can solve various problems in embedding and slicing, so that it can be preserved for a long time, and the number of cultured organoids can be retained to the greatest extent, thereby meeting the requirements of cytological observation in scientific research. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is an isometric structural diagram of the utility model (with the outer tube shown in perspective);

[0018] Figure 2 This is a schematic diagram of the explosion state structure of the utility model (with the outer tube shown in perspective);

[0019] Figure 3 This is a schematic diagram of the structure of the inner hose in the utility model in the exploded state;

[0020] Figure 4 for Figure 3 Schematic diagram of the local enlarged structure of area A in the middle.

[0021] In the figure: 1 outer tube, 2 hose body, 3 tapered portion, 4 tube cover, 5 fixing groove, 6 hanging ear, 7 external threaded tube, 8 internal thread, 9 connecting ring, 10 connecting convex strip. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] See also Figure 1-4 , the utility model provides a technical solution:

[0024] A sleeve type centrifuge tube, as shown in the instruction manual Figure 1 As shown, including:

[0025] An outer tube 1, an inner hose is arranged inside the outer tube 1, and the outer tube 1 and the inner hose are connected to each other through a fixing component. In this embodiment, the inner hose is made of PC (polycarbonate) material;

[0026] The inner hose includes a hose body 2 and a tapered portion 3. The hose body 2 is used to install the ear 6. The hose body 2 and the tapered portion 3 are connected to each other through a connecting assembly. The connecting assembly is used to seal the hose body 2 and the tapered portion 3. The tapered portion 3 is used to place the organoid specimen. In this embodiment, the hose body 2 has a diameter of 12 mm, a thickness of 0.1 mm, and a length of 67 mm. The diameter of the divergent end of the tapered portion 3 is the same as that of the hose body 2. The convergent end of the tapered portion 3 is a round bottom structure with a diameter of 5 mm, and the length of the tapered portion 3 is 8 mm.

[0027] The outer tube 1 is provided with a tube cover 4. In this embodiment, the outer tube 1 and the tube cover 4 are connected by threads.

[0028] The outer tube 1 is provided with a fixing groove 5, and the fixing component includes a hanging ear 6. The hanging ear 6 is fixedly connected to the hose body 2. The outer tube 1 and the inner hose are relatively fixed by inserting the hanging ear 6 into the fixing groove 5. The size of the fixing groove 5 is the same as that of the hanging ear 6. The fixing groove 5 is used to limit the movement of the hanging ear 6 so that after the tube cover 4 is tightened, the fixing groove 5 limits the relative movement between the inner hose and the outer tube 1 through the restriction of the hanging ear 6 by the fixing groove 5. The hanging ear 6 is also used to facilitate the user to take out the inner hose. The width of the fixing groove 5 and the hanging ear 6 are both 5 mm. Therefore, when the hanging ear 6 is inserted into the fixing groove 5, no relative shaking will occur between the hose body 2 and the outer tube 1.

[0029] The connection assembly includes an external threaded tube 7, and the external threaded tube 7 and the internal thread 8 can be engaged with each other so as to seal the hose body 2 and the tapered portion 3 relatively when in use. The external threaded tube 7 is arranged at the bottom of the hose body 2, and the tapered portion 3 is provided with an internal thread 8. The hose body 2 and the tapered portion 3 are sealed by screwing the tapered portion 3 to the external threaded tube 7. The connection assembly also includes a connecting ring 9 and a connecting convex strip 10. The connecting ring 9 is used to install the connecting convex strip 10. As shown in the drawings of the specification, the connecting ring 9 is arranged at the connection between the hose body 2 and the tapered portion 3. The two ends of the connecting ring 9 are not connected end to end. One end of the connecting ring 9 is fixedly connected to the hose body 2 and the tapered portion 3, and the other end of the connecting ring 9 is a movable end so as to facilitate the user to pull during the experiment. The connecting convex strip 10 is arranged at equal intervals on the connecting ring 9 and is used to connect the hose body 2 and the tapered portion 3 to each other. The connecting convex strip 10 is used to prevent the relative movement between the hose body 2 and the tapered portion 3 during the centrifugation process, thereby affecting the sealing.

[0030] Working principle: When in use, load the specimen into the inner hose, then place the inner hose into the outer tube 1 and fix it to the outer tube 1 through the lug 6, put the sleeve centrifuge tube containing the specimen into the rotor slot of the centrifuge, and ensure that the sleeve centrifuge tube is correctly fixed; set the centrifuge time of the centrifuge to 10min, 2000rpm, centrifugal force 600g, and start the centrifuge; after centrifugation, turn off the centrifuge, wait for the rotor to stop rotating, and take out the sleeve centrifuge tube; open the tube cover 4, take out the inner hose, pull the connecting ring 9 by applying radial force to destroy the connecting ridge 10, rotate the conical part 3 to remove the conical part 3, and take out the specimen from the conical part 3, wrap it, and put it into the embedding box.

[0031] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A sleeve-type centrifuge tube, characterized in that: include: An outer tube, wherein an inner hose is arranged inside the outer tube, and the outer tube and the inner hose are connected to each other via a fixing assembly; The inner hose comprises a hose body and a tapered portion, wherein the hose body and the tapered portion are connected to each other via a connecting assembly, and the connecting assembly is used for sealingly connecting the hose body and the tapered portion.

2. A sleeve-type centrifuge tube according to claim 1, characterized in that: The outer tube is provided with a tube cover.

3. A sleeve-type centrifuge tube according to claim 1, characterized in that: The outer tube is provided with a fixing groove, and the fixing assembly includes a hanging ear, and the hanging ear is fixedly connected to the hose body. The hanging ear is inserted into the fixing groove to relatively fix the outer tube and the inner hose.

4. A sleeve-type centrifuge tube according to claim 3, characterized in that: The widths of the fixing groove and the hanging ear are both 5 mm.

5. A sleeve-type centrifuge tube according to claim 1, characterized in that: The connection assembly includes an externally threaded tube, which is arranged at the bottom of the hose body. The tapered portion is provided with internal threads, and the hose body and the tapered portion are sealed by screwing the tapered portion to the externally threaded tube.

6. A sleeve-type centrifuge tube according to claim 5, characterized in that: The connection assembly further comprises a connection ring and a connection ridge, wherein the connection ring is arranged at the connection between the hose body and the tapered portion, and the connection ridge is arranged at equal intervals on the connection ring and is used to connect the hose body and the tapered portion to each other.

7. A sleeve-type centrifuge tube according to claim 1, characterized in that: The tube diameters of the divergent end of the conical portion and the tube diameter of the hose body are both 12 mm, the convergent end of the conical portion is a round bottom structure with a diameter of 5 mm, and the length of the conical portion is 8 mm.

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