Device and convenient box for preparing blood smear for plasmodium standardized detection

Abstract

The utility model provides a device for preparing a blood smear for plasmodium standardized detection and a convenient box. The device comprises a plate body and a cover body matched with the plate body, the plate body comprises four side walls and an inner cavity defined by the four side walls. In the inner cavity, an even number of mutually independent platforms are arranged along the opposite side wall, and each platform is matched with the transparent area of the glass slide in size and shape; the periphery of the platform is a communicated inner cavity bottom; a first protrusion and a second protrusion are arranged at the end, away from the side wall, of each platform and the end, close to the side wall, of each platform. The device integrates multiple functions of flaking, dyeing and airing, and is a multifunctional device. The convenient box comprises the device for preparing the blood smear for plasmodium standardized detection, a cleaning brush and a blood film preparation template.

Description

Technical Field

[0001] The utility model belongs to the field of biological detection devices, and more specifically, relates to a device and a convenient box for preparing blood smears for standardized detection of malarial parasites. Background Art

[0002] Microscopic examination of malarial parasites is the gold standard for clinical diagnosis of malaria. Among them, the preparation of high-quality blood smears with thick and thin blood films is one of the key factors to ensure the accuracy of the test results. The preparation of blood smears is described in the relevant industry standards "Blood Smear Microscopic Examination Method for Plasmodium Detection" (WS / T 569-2017) and "Diagnosis of Malaria" (WS259-2015). Specifically, take a slide, apply the blood drop in the lower left corner of the slide to the left of the center of the slide, and apply it from the inside to the outside in a circle with a diameter of 8mm to 10mm. Use a dry cotton ball to wipe off the blood stain on the lower left corner of the slide, and then flatten the lower edge of the slide against the midline of the slide. When the blood spreads to both sides between the slide and the slide to a width of about 20mm, keep the two slides at 25° to 35° and push forward quickly from right to left to form a tongue-shaped thin blood film.

[0003] However, in actual operation, the above description alone cannot enable the operator to correctly grasp the position and size of the blood film, and the above standards do not involve and consider the sealing of the blood film after preparation. At the same time, for Plasmodium, the "Technical Program for Preventing Imported Retransmission after Elimination of Malaria" (2020) issued by the Centers for Disease Control and Prevention requires that the malaria microscopy skills possessed by disease control agencies also include density counting. The white blood cell density counting method currently recommended by the World Health Organization requires that the prepared blood smear must be a specific high-quality thick blood film, and the size requirement is different from the domestic diameter of 8mm to 10mm. Practical operation has proved that the former's 12mm can better meet practical needs.

[0004] Malaria microscopic examination skills, including blood smear preparation and malarial parasite microscopy, are skills that require a certain amount of time and repeated practice to master, and high-quality blood smears need to be prepared within 1 hour of blood collection. All reported cases of malaria are imported cases, and their distribution is characterized by irregular points, irregular times, and imports from all over the country. As a result, in most areas, in theory, testing personnel need to master this skill in order to ensure timely and accurate diagnosis of each malaria case, but in actual work, there is no opportunity, and even no need to spend a lot of time practicing this skill.

[0005] Therefore, there is an urgent need in the art to provide a malaria blood smear making device that integrates the multi-functions of making, dyeing and drying the smears, so as to facilitate the use of detection personnel, especially beginners. Utility Model Content

[0006] The utility model aims to provide a device and a convenient box for preparing blood smears for standardized detection of malarial parasites; the device can be used for integrated operations of smear preparation, staining and drying.

[0007] In a first aspect of the utility model, a device for preparing a blood smear for standardized detection of malarial parasites is provided, the device comprising: a plate body 1, a cover body 2 matched with the plate body 1; the plate body 1 comprises four side walls 11 and an inner cavity 12 surrounded by the plate body 1; an even number of mutually independent rectangular platforms 13 are arranged in the inner cavity 12 along the opposite side walls, and each platform 13 matches the size and shape of the transparent area 42 of the slide glass; two first protrusions 14 of the same vertical height are arranged at one end of each platform 13 away from the side wall 11, and the distance between the two is less than the short side length of the slide glass (preferably, the distance between the two is 11±3mm, 11±2mm or 11±1mm); accordingly, two second protrusions 15 of the same vertical height are arranged at one end of each platform adjacent to the side wall 11 or on the side wall 11, and the distance between the two can accommodate the transparent area 42 of the slide glass. The short side of the glass slide (preferably, the net distance between the two is not less than 25.4mm, which can be increased by 0-6mm, preferably by 2mm); the vertical height of the first protrusion 14 is lower than that of the second protrusion 15 (preferably 1.2-2.4mm lower); the first protrusion 14 and the second protrusion 15 on the same platform are used to fix the glass slide; on two adjacent platforms arranged along the same side wall, the spacing between two adjacent first protrusions 14 is smaller than the short side of the glass slide (preferably the distance is 9-24mm, for example 10, 12, 15, 16, 18, 20, 22mm), the spacing between two adjacent second protrusions 15 is smaller than the short side of the glass slide (preferably the distance is 9-24mm, for example 10, 12, 15, 16, 18, 20, 22mm), and the first protrusions and the second protrusions on adjacent platforms are used to control the flow direction of the staining solution and the elution solution.

[0008] In one or more embodiments, the vertical height of the first protrusion 14 is lower than the height of the glass slide.

[0009] In one or more embodiments, the top surface of the platform 13 includes: a thick blood film marking area 16, a thin blood film marking area 17, and a marking area 19 that demarcates the frosted edge of the glass slide and the transparent area; the thick blood film marking area 16 is provided with a circle 161 and a center 162; when the glass slide is located on the top surface of the platform, through the glass slide, the center 162 indicates the thick blood film blood-setting point on the glass slide, and the circle 161 indicates the thick blood film coating area on the glass slide; the thin blood film marking area is provided with a thin line 171, and when the glass slide is located on the top surface of the platform, through the glass slide, the thin line indicates the starting position of the thin blood film, and the center of the thin line is the thin blood film blood-setting point (indicating the position where blood is added to prepare the thin blood film).

[0010] In one or more embodiments, the top surface of the platform 13 includes: a recessed structure 18, which is inclined downward in depth and is used to insert a slide and dry the washed blood film; the inclination of the recessed structure downward in depth is 60-85° with the horizontal direction; such as 65°, 70°, 75°, 80°.

[0011] In one or more embodiments, the width of the recessed structure is 1.5-3 mm, such as 1.6, 1.8, 2, 2.2, 2.5, 2.8 mm. The groove depth of the recessed structure is 6-18 mm, such as 8, 9, 10, 11, 12, 13, 14, 15, 16 and 18 mm.

[0012] In one or more embodiments, the diameter of the circle 161 is 10±3 mm or 10±2 mm, preferably 10±1 mm.

[0013] In one or more embodiments, the number of the platforms 13 is 2 to 60; preferably, they are arranged along the opposite side walls, with 1 to 30 platforms on each side.

[0014] In one or more embodiments, the number of the platforms 13 is 4 to 50, 6 to 40, 8 to 30, 10 to 40, 12 to 30, such as 14, 16, 18, 20, 22, 24, 26, 28. Preferably, they are arranged along the opposite side walls, with 2 to 25, 3 to 20, 4 to 15, 5 to 20, 6 to 15, such as 7, 8, 9, 10, 11, 12, 13, 14 on each side.

[0015] In one or more embodiments, the cover body 2 includes: four side edges 21 and a top plate 22; the cover body 2 and the plate body 1 can be opened and closed with each other.

[0016] In one or more embodiments, the upper surface of the top plate 22 includes: a thick blood film marking area 26 and a thin blood film marking area 27; the thick blood film marking area 26 is provided with a circle 261 and a center 262, and the thin blood film marking area 2 is provided with a thin line 271.

[0017] In one or more embodiments, the number of the thick blood film identification area two 26 and the thin blood film identification area two 27 is the same as the number of the thick blood film identification area one 16 and the thin blood film identification area one 17.

[0018] In one or more embodiments, on the upper top surface of the top plate 22 of the cover body 2, between the thick blood film identification area 26 and the side edge 21, there is also a second identification area 28 that demarcates the frosted edge of the slide and the transparent area.

[0019] In one or more embodiments, the marking area 28 separating the frosted edge and the transparent area of ​​the glass slide is used to determine the placement position of the glass slide.

[0020] In one or more embodiments, the thick blood film marking area 26, the thin blood film marking area 27, and the frosted edge of the slide and the transparent area boundary marking area 28 are concave by 0.8-1 mm or roughened and anti-slip to fix the slide.

[0021] In one or more embodiments, the outer wall of the side wall 11 of the plate body 1 is provided with a circle of convex structures for cooperating with the cover body 2 to buckle therewith.

[0022] In one or more embodiments, the convex structure convexes by 2 mm and may be 20 mm away from the bottom.

[0023] In one or more embodiments, the side wall is 230 mm long, 150 mm wide and 40 mm high; the length, width and height may fluctuate up and down by 30%, 20%, 15%, 10%, 5% or 2%.

[0024] In one or more embodiments, the total height of the cover body 2 and the plate body 1 after closing is 40-50 mm; the length, width and height may fluctuate up and down by 30%, 20%, 15%, 10%, 5% or 2%; preferably 45 mm.

[0025] In one or more embodiments, the overlap between the cover body 2 and the plate body 1 is at least 8 mm, preferably at least 10 mm.

[0026] In one or more embodiments, at least one drain outlet 3 is disposed on the side wall 11 of the plate body 1 for discharging dyeing liquid, eluting liquid or cleaning liquid when the device is large in size.

[0027] In another aspect of the utility model, a convenient box for preparing a blood smear for standardized detection of Plasmodium is provided, the convenient box comprising: any of the above-mentioned devices for preparing a blood smear for standardized detection of Plasmodium; and a cleaning brush, the cleaning brush being used to clean residual impurities when cleaning the device.

[0028] In one or more embodiments, the residual impurities include (but are not limited to): dyeing liquid or particulate impurities.

[0029] In one or more embodiments, the convenience box also includes: a blood film preparation template.

[0030] In one or more embodiments, the blood film preparation template is an A4 version.

[0031] In one or more embodiments, the blood film preparation template is a scannable electronic version.

[0032] In one or more embodiments, the convenience box further includes: a push sheet for spreading the blood film.

[0033] In one or more embodiments, the cleaning brush is a sponge brush.

[0034] Other aspects of the present invention will be apparent to those skilled in the art from the disclosure herein. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 , Schematic diagram of a malarial blood smear with a thick blood film and a thin blood film (applied on a slide).

[0036] Figure 2 , a schematic top view of the plate body of the device for preparing blood smears for standardized detection of Plasmodium.

[0037] Figure 3 , a schematic top view of the top surface of the cover of the device for preparing a blood smear for standardized detection of Plasmodium.

[0038] Figure 4 , a schematic diagram of the three-dimensional structure of the plate body of the device for preparing blood smears for standardized detection of Plasmodium.

[0039] Figure 5 , a schematic diagram of the three-dimensional structure of the cover of the device for preparing a blood smear for standardized detection of Plasmodium.

[0040] Figure 6 , schematic diagram of a fixing method of the glass slide by the first protrusion and the second protrusion when the glass slide is placed on the plate body and the platform.

[0041] Figure 7 , Schematic diagram of the placement of slides during the staining stage in the preparation process.

[0042] In the figure, each reference numeral is described as follows:

[0043] 1: Plate body;

[0044] 11: side wall;

[0045] 12: Lumen;

[0046] 13: Platform;

[0047] 14: first protrusion;

[0048] 15: second bulge;

[0049] 16: thick blood film identification area 1; 161: circle 1; 162: center of circle 1;

[0050] 17: thin blood film marking area 1; 171: thin line 1;

[0051] 18: Concave structure;

[0052] 19: Identification area 1 that separates the frosted edge and transparent area of ​​the slide;

[0053] 2: Cover body;

[0054] 21: side;

[0055] 22: top plate;

[0056] 26: thick blood film identification area 2; 261: circle 2; 262: center of circle 2;

[0057] 27: thin blood film marking area 2; 271: thin line 2;

[0058] 28: Marking area 2 that separates the frosted edge and transparent area of ​​the slide;

[0059] 3: Drain pipe outlet.

[0060] 4: glass slide; 41: frosted edge; 42: transparent area; 43: thick blood film; 44: thin blood film. DETAILED DESCRIPTION

[0061] After in-depth research and analysis, the designer provides a device for preparing blood smears for standardized detection of malarial parasites, including: a plate body, and a cover body matched with the plate body. The design of the plate body and the cover body fully considers the standardized production of malarial blood smears, and integrates the multi-functions of making, dyeing, and drying the slides into one, which is a multifunctional device. The utility model also includes a convenient box, which includes the device for preparing blood smears for standardized detection of malarial parasites and a cleaning brush.

[0062] When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional views showing the device structure will not be partially enlarged according to the general scale, and the schematic views are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0063] As shown in the present invention and the claims, unless the context clearly indicates an exception, the words "a", "an", "an" and / or "the" do not specifically refer to the singular but may also include the plural.

[0064] For the convenience of description, the present invention may use spatial relationship words such as "under", "below", "below", "below", "above", "on", etc. to describe the relationship between an element or feature shown in the drawings and other elements or features. It will be understood that these spatial relationship words are intended to include other directions of the device in use or operation, in addition to the direction depicted in the drawings. For example, if the device in the drawings is turned over, the direction of the element described as being "under" or "under" or "below" other elements or features will be changed to "above" the other elements or features. Thus, the exemplary words "under" and "below" can include both the upper and lower directions. The device may also have other orientations (rotated 90 degrees or in other directions), so the spatial relationship descriptors used herein should be interpreted accordingly. In addition, it will be understood that when a layer is referred to as being "between" two layers, it can be the only layer between the two layers, or there may be one or more layers in between.

[0065] In the context of the present invention, the structure described as a first feature "on" a second feature may include an embodiment in which the first and second features are formed in direct contact, and may also include an embodiment in which another feature is formed between the first and second features, so that the first and second features may not be in direct contact.

[0066] Generally speaking, the terms "include" and "comprises" only indicate that the steps and elements that have been explicitly identified are included, and these steps and elements do not constitute an exclusive list. The method or device may also include other steps or elements.

[0067] The malaria blood smear preparation device of the utility model fully considers the reality and actual needs of a wide range of regions, is designed according to the description of the blood smear preparation method and process in the detection standard and the practical experience in daily work, and integrates the multi-functions of preparation, dyeing and drying, so that beginners can operate it immediately. Whether beginners or ordinary experimenters, they can prepare high-quality blood smears by relying on the device of the utility model.

[0068] The present invention is further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer.

[0069] Example 1: A device for preparing blood smears for standardized detection of malarial parasites

[0070] In this embodiment, a device for preparing a blood smear for standardized detection of Plasmodium is provided. The preparation of the blood smear for standardized detection of Plasmodium is performed on a glass slide.

[0071] like Figures 2 to 5 As shown, a device for preparing blood smears for standardized detection of malarial parasites is provided, which is divided into two parts, upper and lower parts, including: a plate body 1, and a cover body 2 matched with the plate body 1.

[0072] 1. Board body

[0073] The plate body 1 includes four side walls 11 and an inner cavity 12 surrounded by them; in the inner cavity 12, 12 (6 on each side) independent rectangular platforms 13 are arranged along the opposite side walls, and the top surface thereof is rectangular, and the overall shape is a cuboid. Each platform 13 matches the size and shape of the transparent area 42 of the slide; the surrounding of the platform is the bottom of the inner cavity (which is a concave bottom relative to the platform).

[0074] Optionally, a drain pipe outlet 3 is further provided on the side wall 11 of the plate body 1 .

[0075] Two first protrusions 14 of the same vertical height are provided at one end of each platform 13 away from the side wall 11, and the distance between the two is less than the length of the short side of the slide (the distance between the two is 11 mm); accordingly, two second protrusions 15 of the same vertical height are provided on the side wall 11 adjacent to each platform, and the distance between the two can accommodate the short side of the slide (the distance between the two is 25.6 mm); the vertical height of the first protrusion 14 is lower than that of the second protrusion 15 (1.5 mm lower); the first protrusion 14 and the second protrusion 15 on the same platform are used to fix the slide; on two adjacent platforms arranged along the same side wall, the distance between two adjacent first protrusions 14 is less than the short side of the slide, and the distance between two adjacent second protrusions 15 is less than the short side of the slide, and the first protrusions 14 and the second protrusions 15 on the adjacent platforms are used to control the flow direction of the staining solution and the elution solution so that they flow into the inner cavity 12 without causing pollution to the area outside the device.

[0076] The top surface of the platform 13 includes: a thick blood film marking area 16, a thin blood film marking area 17, and a marking area 19 which is the boundary between the frosted edge and the transparent area of ​​the slide.

[0077] The thick blood film marking area 16 is provided with a circle 161 and a center 162; when the glass slide is located on the top surface of the platform, through the glass slide, the center 162 indicates the thick blood film blood placement point on the glass slide, and the circle 161 indicates the thick blood film coating area on the glass slide. The diameter of the circle 161 is 12 mm. The circle 1 and the center 1 are marked with darker or brighter colors and clear lines.

[0078] The thin blood film marking area 1 is provided with a thin line 171. When the glass slide is located on the top surface of the platform, the thin line 1 indicates the starting position of the thin blood film through the glass slide, and the center of the thin blood film is the blood placement point.

[0079] The top surface of the platform 13 also includes a recessed structure 18, which is inclined downward and deep, and is used to insert a glass slide and dry the washed blood film. The recessed structure is inclined downward and deep at an angle of 75° to the horizontal direction. The width of the recessed structure is 2mm, and the groove depth is 10mm.

[0080] 2. Cover

[0081] The cover body 2 includes four side edges 21 and a top plate 22 ; the cover body 2 and the plate body 1 can be opened and closed with each other.

[0082] The upper top surface of the top plate 22 includes: a thick blood film identification area 26 and a thin blood film identification area 27; the thick blood film identification area 26 is provided with a circle 261 and a center 262, and the thin blood film identification area 2 is provided with a thin line 271; the number of the thick blood film identification area 26 and the thin blood film identification area 27 is the same as the number of the thick blood film identification area 16 and the thin blood film identification area 17. Between the thick blood film identification area 26 and the side edge 21, it also includes: an identification area 28 that demarcates the frosted edge of the slide and the transparent area, which can be used to determine the placement position of the slide to avoid the wrong direction. The thick blood film identification area 26, the thin blood film identification area 27, and the identification area 28 that demarcates the frosted edge of the slide and the transparent area are concave 0.8-1mm or roughened for anti-slip treatment to fix the slide.

[0083] The entire device is 230mm long, 150mm wide, and less than 50mm (about 40mm) in total height. The total height of the side wall of the plate body 1 is 35mm. The outer wall of the side wall 11 of the plate body 1 is provided with a circle of convex structures to match the cover body 2 and snap it together; the convex structure bulges outward by 2mm, is 20mm away from the bottom, and has an oblique support at the bottom to support the cover or fit it with the cover. The total height of the cover body 2 is about 20mm, and when the cover body 2 and the plate body 1 are closed, the overlap between the cover body 2 and the plate body 1 is about 10mm.

[0084] The structure of the cover body 2 that is buckled with the plate body 1 can be in two steps, with the first step being engaged with the uppermost part of the outer convex structure and the second step being engaged with the platform on the outer frame.

[0085] Example 2: Accurate positioning of blood film on glass slide

[0086] In this embodiment, a preferred method for accurately positioning the position of a blood film on a glass slide when preparing a blood film is provided.

[0087] The most commonly used slide model in practice is the 7105 single-end frosted slide, which is 25.4mm×76.2mm×1-1.2mm in size. Figure 1 .

[0088] The position of the thick blood film of the malarial blood smear is usually located at one-third of the slide. As an embodiment, the rightmost edge of the thick blood film is at one-third, and the distance between it and the frosted edge is 5.4mm (25.4-20=5.4mm), and the left edge of the thick blood film is at 35.4mm (25.4+10=35.4mm).

[0089] As an implementation method, the thin blood film starts at one-half of the slide, such as 38.1 mm. However, the distance between this position and the thick blood film positioned above is too short (38.1-35.4=2.7 mm). After the thick and thin blood films are prepared and fully dried, methanol is used to fix the thin blood film, and the methanol is not allowed to touch the thick blood film. However, if the distance between the two is too short, the solution is easily stained on the thick blood film by mistake, making it completely or partially unobservable after staining. Therefore, the starting position of the thin blood film is adjusted. According to practical experience, the film spacing>5 mm. In addition, in order to make the thin blood film long enough (the row mark is 20-25 mm), and to prevent the thin blood film from being too close to the left edge when reading the film, the head of the thin blood film should be>5 mm away from the short side of the slide. Therefore, in addition to>5 mm, the film spacing also needs to be <10.7 mm (76.2-35.4-25-5=10.7 mm). If the diameter of the thick blood film is 12 mm (WHO standard), the distance between the thick and thin blood films should be less than 8.7 mm. At the same time, the total length from the right edge of the thick blood film to the left tail of the thin blood film should be less than 50 mm (slide length).

[0090] Based on the above, the positions of the thick and thin blood films (with the frosted and non-frosted areas as 0) are determined from right to left (the diameter of the thick blood film is 12 mm) as shown in Table 1.

[0091] Table 1. Relevant positions of key sites in standard malaria blood smears

[0092]

[0093] During implementation, the second thick blood film marking area and the second thin blood film marking area on the platform and the cover can be accurately positioned according to Table 1.

[0094] In addition, the glass slide may also be a glass slide without frosted edges. When a glass slide without frosted edges is used to coat the blood film, the short side of another glass slide of the same model may be used to measure the position of the rightmost edge of the thick blood film (the right edge of the thick blood film) on the long side before coating. The starting point of the thick blood film may then be determined based on the position of the thick blood film.

[0095] Example 3. Use of the device of Example 1

[0096] like Figure 4 and Figure 6As shown, a glass slide for preparing a blood smear is placed on a platform and a frame, and a first protrusion and a second protrusion are used to fix the glass slide, and the short side of the transparent area of ​​the glass slide abuts against the second protrusion, and the frosted sides of the long side of the glass slide are clamped by the first protrusion. After the glass slide is placed, the thick blood film marking area 1 and the thin blood film marking area 1 on the platform can be seen through. The height of the first protrusion is not higher than the thickness of the glass slide (1-2mm), so that when staining, it will not produce adsorption due to contact with the dye, causing the dye to flow away from the glass slide containing the blood film.

[0097] When placing, the transparent area of ​​the slide is placed on the platform (preferably placed according to the position in the precise positioning of Example 2), and the frosted edge of the slide is suspended about 15 mm outside the frame to facilitate the hand-held slide.

[0098] When preparing a blood smear, wear gloves, pinch the two sides of the frosted edge of the slide (20mm edge) with the index finger and thumb, and place it on the platform or cover of the plate body of the device. Except for the frosted edge, the other parts overlap with the platform. One platform is placed with a slide, and the first protrusion and the second protrusion are used for fixing as above. With the thick blood film marking area 1 (including circle 1 and center 1) and thin blood film marking area 1 (including thin line 1) on the platform, the thick blood film and thin blood film can be clearly observed through the slide. Use a pipette to draw 6μL and 1-1.5μL of blood respectively and place them at the center of circle one and the midpoint of thin line one. If the thick blood film takes less than 6μL of blood, the size of the blood film can be adjusted according to the amount of blood. The amount of blood should not be less than 4μL and the maximum is 6μL. When preparing thin blood films, a larger amount is usually used (1.5μL-2μL). The blood can be dropped closer to the thick blood film, but the distance from the edge of the thick blood film must be ≥5mm. The amount of blood should not be more than 2μL and the minimum is 1μL.

[0099] Then, hold the slide with one hand and hold the slide pusher with the other hand (or take another clean slide), and use one corner to quickly move counterclockwise from the inside to the outside to cover the entire circle with the blood drop in the center of the circle, and finally return to the center of the circle and lift the slide pusher vertically.

[0100] Pick up the glass slide coated with thick blood film, pinch the two short sides of the glass slide with the thumb and index finger (or middle finger), with the frosted side at the end of the index finger, wipe the blood stains left after the thick blood film is pushed on the push piece, turn the push piece over, and make the short side without blood stains contact the glass slide. Adjust the position of the glass slide and the push piece so that all the short sides of the glass slide and the push piece are parallel to each other. After the long sides are parallel to each other, pull the push piece back to make it contact with the blood drop on the thin line. When the blood drop spreads to 20mm-23mm along the edge of the push piece, immediately push the push piece forward at a uniform speed to spread the blood drop on the glass slide to prepare a thin blood film. Put the glass slide (blood smear) containing thick and thin blood films back to its original place to dry naturally.

[0101] Alternatively, after the thick blood film is prepared, blood drops for preparing a thin blood film may be added.

[0102] After the blood film is fully dried, pinch the two sides of the frosted edge of the blood smear (the 20mm long edge), with the thin blood film at the bottom, and the angle with the horizontal direction greater than 45° and less than 90°, and use a pipette to absorb methanol to completely soak the thin blood film (you can also place the methanol in a beaker with a diameter wider than the short side of the slide, and the liquid level is less than 33mm (that is, it can completely immerse the thin blood film, but there is a distance of about 5mm from the thick blood film, and the methanol will not touch the thick blood film). Soak the thin blood film vertically in it for 1 second and then take it out, dry the excess methanol on absorbent paper and lay it flat). After the methanol evaporates, place the blood smears on the platforms respectively, and gently cover the blood film with the freshly prepared staining solution for staining (about 3mL / sheet). After staining, pick up the blood smear and the staining solution together and put them in water for rinsing. Alternatively, place the blood smear between the two platforms, with the end with the frosted edge on the second protrusion and the other end on the first protrusion (preferably one protrusion for each platform, such as Figure 7 ). Since the vertical height of the second protrusion is higher than that of the first protrusion, any liquid on the smear can flow out and enter the inner cavity as soon as possible. After the blood smear is stabilized, it is stained. After the staining is completed, the eluent is sucked from the frosted end with a pipette to wash away the staining liquid on the blood film, and all the liquid flows into the inner cavity outside the platform in the plate body.

[0103] The marking areas on the cover are only for the convenience of preparing blood smears and are not stained.

[0104] After rinsing off the stain, place the blood smear with the frosted edge facing down, insert the blood film downward into the concave structure (groove) of the platform, and dry the blood film for microscopic examination.

[0105] Example 4: Convenient kit for preparing blood smears for standardized detection of Plasmodium

[0106] A container is provided, preferably a square box (package), into which the device for preparing a blood smear for standardized detection of Plasmodium parasites according to Example 1 is placed; and a sponge brush is placed therein for cleaning residual impurities when cleaning the device. Optionally, a blood film preparation template (A4 version) is also placed therein.

[0107] The convenience box integrates the main devices and materials required for actual film-making operations, making it easy to use on site.

[0108] The above-mentioned embodiments only express several implementation methods of the utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the utility model, and these all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A device for preparing blood smears for standardized detection of malarial parasites, characterized in that: The device comprises: a plate body (1), and a cover body (2) matched with the plate body (1); The plate body (1) comprises four side walls (11) and an inner cavity (12) surrounded by the side walls; in the inner cavity (12), an even number of mutually independent rectangular platforms (13) are arranged along the opposite side walls, and each platform (13) matches the size and shape of the transparent area (42) of the slide glass; Two first protrusions (14) of the same vertical height are arranged at one end of each platform (13) away from the side wall (11), and the distance between the two protrusions is less than the length of the short side of the glass slide; correspondingly, two second protrusions (15) of the same vertical height are arranged at one end of each platform adjacent to the side wall (11) or on the side wall (11), and the distance between the two protrusions can accommodate the short side of the glass slide; the vertical height of the first protrusion (14) is lower than that of the second protrusion (15); the first protrusion (14) and the second protrusion (15) on the same platform are used to fix the glass slide; on two adjacent platforms arranged along the same side wall, the distance between the two adjacent first protrusions (14) is less than the short side of the glass slide, and the distance between the two adjacent second protrusions (15) is less than the short side of the glass slide, and the first protrusions and the second protrusions on the adjacent platforms are used to control the flow direction of the staining liquid and the elution liquid.

2. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 1, characterized in that: The top surface of the platform (13) includes: a thick blood film identification area (16), a thin blood film identification area (17), and an identification area (19) that demarcates the frosted edge of the slide and the transparent area; The thick blood film marking area 1 (16) is provided with a circle 1 (161) and a center 1 (162); when the glass slide is located on the top surface of the platform, through the glass slide, the center 1 (162) indicates the thick blood film bleeding point on the glass slide, and the circle 1 (161) indicates the thick blood film coating area on the glass slide; The thin blood film marking area is provided with a thin line (171). When the glass slide is located on the top surface of the platform, the thin line indicates the starting position of the thin blood film through the glass slide, and the center of the thin blood film is the blood placement point.

3. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 1, characterized in that: The top surface of the platform (13) comprises: a recessed structure (18) which is inclined downward and deep, and is used for inserting a glass slide and drying the washed blood film; the inclination of the recessed structure is 60-85 degrees with the horizontal direction.

4. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 1, characterized in that: The number of the platforms (13) is 2 to 60.

5. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 1, characterized in that: The cover body (2) comprises: four side edges (21) and a top plate (22); the cover body (2) and the plate body (1) can be opened and closed with each other.

6. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 5, characterized in that: The upper surface of the top plate (22) includes: a second thick blood film marking area (26) and a second thin blood film marking area (27); The second thick blood film marking area (26) is provided with a second circle (261) and a second center (262), and the second thin blood film marking area is provided with a second thin line (271).

7. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 6, characterized in that: On the upper surface of the top plate (22) of the cover body (2), between the thick blood film identification area 2 (26) and the side edge (21), there is also a second identification area (28) which demarcates the frosted edge of the slide and the transparent area.

8. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 1, characterized in that: The outer wall of the side wall (11) of the plate body (1) is provided with a circle of convex structures for cooperating with the cover body (2) and buckling therewith.

9. The device for preparing a blood smear for standardized detection of malarial parasites according to claim 1, characterized in that: The side wall (11) of the plate body (1) is provided with at least one liquid discharge pipe outlet (3) for discharging dyeing liquid, eluting liquid or cleaning liquid.

10. A convenient kit for preparing blood smears for standardized detection of Plasmodium, characterized in that: The convenience box comprises: The device for preparing a blood smear for standardized detection of malarial parasites according to any one of claims 1 to 9; A cleaning brush, the cleaning brush being used to clean residual impurities when cleaning the device; Blood film preparation template, which can be photocopied or scanned into an electronic version, is used to prepare a large number of blood smears at the same time.

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