Device for propagating larva parasitic wasps

Abstract

The device comprises a non-transparent bottom box and a transparent cover, the non-transparent bottom box and the transparent cover are sequentially arranged from bottom to top and can be sequentially connected in a sealed mode, the non-transparent cover and the non-transparent bottom box are open, and a first inlet communicated with an inner cavity of the non-transparent bottom box is formed in the side wall of the non-transparent bottom box. The transparent cover is provided with a second inlet corresponding to the inner cavity so that the second inlet can be communicated with the inner cavity when the transparent cover covers the opening of the non-transparent bottom box, and the non-transparent cover is provided with a third inlet corresponding to the second inlet. The first plug can block the first inlet, the first transparent adapter body can be detachably communicated with the first inlet, the second plug can block the second inlet, and the second transparent adapter body can penetrate through the third inlet and is detachably communicated with the second inlet. The device has remarkable advantages in labor time, parasitic rate and cocoon harvesting rate, the propagation cost can be obviously reduced, and the propagation efficiency can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of parasitic wasp propagation devices, in particular to a device for propagating larval parasitic wasps. Background Art

[0002] Pests of the Lepidoptera family Noctuidae are widely distributed in various countries and regions. Representatives include: Spodoptera lituraFabricius, Spodoptera frugiperda, Spodoptera exigua and Helicoverpa armigera. They have large appetites, varied diets and strong reproductive capacity. The larvae cause holes or gaps in plant leaves, which can cause plant death in severe cases. Food crops, garden plants, vegetables, fruit plants, etc. in most parts of China are harmed by Noctuidae pests, causing huge economic losses and serious social impacts on agricultural production and the ecological environment.

[0003] At present, the method of controlling Noctuidae pests is still mainly chemical control, but the long-term use of chemical pesticides has led to excessive pesticide residues, increased pest resistance, and the destruction of ecological balance. As an important part of biological control, the control of natural enemies of pests is a green and safe long-term pest control method. As natural enemies of pests, parasitic wasps are an important biological control resource. After large-scale production and packaging, they can be used as pest biological control products for green pest control, which has great application value.

[0004] Microplitis manilae and Meteorus pulchricornis can parasitize Lepidoptera larvae, and play an important role in the prevention and control of various Lepidoptera pests such as Spodoptera litura, cotton bollworm, fall armyworm, beet armyworm, gypsy moth, etc., and have high biological control potential. Both parasitic wasps lay eggs in the bodies of Lepidoptera larvae. After being parasitized, the Lepidoptera larvae rapidly reduce their food intake. After the parasitic wasp larvae mature in their bodies, they drill out of the Lepidoptera larvae and cocoon, and the Lepidoptera larvae eventually die. This greatly reduces the degree of damage caused by pests to plants.

[0005] At present, there are few studies on the scale propagation technology of Manila side groove braconids and mole septoria at home and abroad, especially in terms of parasitism containers, rapid separation methods of parasitoids and hosts, and host group breeding susceptibility to disease and death, resulting in high propagation costs. The present invention / utility model mainly makes innovative designs for the parasitism and breeding containers of parasitoids and hosts, so that they meet the needs of large-scale production and carry out parasitoid propagation at low cost and high efficiency. Summary of the invention

[0006] The technical problem solved by the utility model is to provide a device for multiplying larval parasitic wasps.

[0007] The specific technical solutions are as follows:

[0008] A device for propagating larval parasitic wasps comprises a non-transparent bottom box, a transparent cover and a non-transparent cover which are arranged in sequence from bottom to top and can be sealed and connected in sequence. The non-transparent bottom box is arranged with an opening, and a first inlet connected to its internal chamber is provided on the side wall of the non-transparent bottom box, and a second inlet corresponding to the internal chamber is provided on the transparent cover so that when the transparent cover is arranged on the opening of the non-transparent bottom box, the second inlet is connected to the internal chamber, and a third inlet corresponding to the second inlet is provided on the non-transparent cover, and further comprises a first plug capable of blocking the first inlet and a first transparent adapter capable of being detachably connected to the first inlet, and further comprises a second plug capable of blocking the second inlet and a second transparent adapter capable of passing through the third inlet and being detachably connected to the second inlet, and the middle part of the transparent cover is an open square opening and the square opening is covered with a gauze with a mesh number of 60-100.

[0009] Preferably, the first inlet is a first circular port, the second inlet is a second circular port, the third inlet is a third circular port, the first transparent adapter is a first transparent adapter tube, the second transparent adapter is a second transparent adapter tube, the first transparent adapter tube and the second transparent adapter tube are a common transparent adapter tube, the two opposite ends of the common transparent adapter tube are a first end and a second end, the first end is used to connect to the first circular port, and the second end is used to connect to the second circular port, the first circular port is directly formed on the side wall, the first end is in a circular port shape matching the first circular port, and its outer sleeve A rubber ring is provided for sealing and connecting to the first circular opening, a through hole is provided on the transparent cover, a hollow circular ring protruding upward is provided at the edge of the through hole, the through hole and the internal channel of the hollow circular ring form the second circular opening, two opposite arc-shaped clamping protrusions are provided on the outer tube wall of the second end, and two opposite arc-shaped clamping grooves corresponding to the two arc-shaped clamping protrusions are provided on the inner side wall of the hollow circular ring, so that the two arc-shaped clamping protrusions of the second end are inserted into the second circular opening and can be rotatably clamped to the two arc-shaped clamping grooves, and the third circular opening of the non-transparent cover can be sleeved on the outer peripheral side of the hollow circular ring.

[0010] Preferably, the lower end of the hollow ring is detachably connected to the transparent cover by bonding.

[0011] Preferably, the shared transparent transfer tube is a curved tube.

[0012] Preferably, the first circular opening and the second circular opening have the same size.

[0013] Preferably, the first plug is a first round-mouth plug, and the second plug is a second round-mouth plug.

[0014] Preferably, the first round-mouth plug and the second round-mouth plug are a common round-mouth plug.

[0015] Preferably, the non-transparent bottom box, the transparent cover and the non-transparent cover are connected in sequence by snap-fit ​​connection.

[0016] Preferably, the dimensions of the non-transparent bottom box are: 20-60 cm in length, 15-50 cm in width, and 4-10 cm in height.

[0017] The device for propagating larval parasitic wasps provided by the utility model has the following technical effects:

[0018] The device, when in use, comprises the following:

[0019] ① Place a quantitative amount of hosts of appropriate age and appropriate amount of host feed in the inner chamber of the non-transparent bottom box, put in a cotton ball soaked in honey water, put the transparent cover on the non-transparent bottom box, and close the second round opening on the transparent cover with the second round opening plug;

[0020] ② Remove the first round mouth plug at the first round mouth on the side wall of the non-transparent bottom box, connect the first transparent adapter tube, introduce the parasitic wasp into the non-transparent bottom box (using the light-repelling and upward-moving properties of the parasitic wasp), and finally block the first round mouth with the first round mouth plug;

[0021] ③ The non-transparent bottom box of step ② is placed in an insect breeding room under certain conditions, and the parasitic wasps complete the parasitic process in the box;

[0022] ④After the parasitic process is completed, put the non-transparent cover on the transparent cover;

[0023] ⑤ Remove the second round-mouth plug and connect the second transparent transfer tube, use the phototaxis and upward attraction of the parasitic wasp to lead it into the transfer tube, and then lead it out of the device.

[0024] The above steps ① to ⑤ complete a parasitic process, and the entire parasitic process is cycled to achieve the purpose of rapid expansion of parasitic wasps. The specific effects are as follows:

[0025] ① It can quickly and non-destructively separate the host and the parasitic wasp. Compared with the traditional separation method (manual catching, sucking with a sucker), it is not only more efficient, but also does not cause any damage to the parasitic wasp;

[0026] ② Compared with the existing breeding methods, the breeding method carried out by the device has significant advantages in terms of working hours, parasitism rate and cocoon collection rate, which can significantly reduce the cost of expansion and improve the efficiency of expansion;

[0027] ③ The problem of susceptibility of armyworm larvae to diseases during large-scale propagation (viruses, fungi, and bacteria infecting armyworm larvae) has been solved, thus ensuring the stability of large-scale propagation.

[0028] ④ It solves the problem of self-mutilation of noctuid larvae during large-scale propagation, reduces propagation losses, and reduces the proportion of secondary contamination of noctuid larvae by pathogens caused by the decay of the insect bodies after self-mutilation of noctuid larvae.

[0029] ⑤ The problem of noctuid larvae feeding on parasitic wasp cocoons during large-scale propagation was solved, thus reducing propagation losses. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 A schematic diagram of the structure of a device for multiplying larval parasitic wasps provided by the utility model;

[0031] Figure 2 It is a schematic diagram of the related structure including a non-transparent bottom box and a transparent cover;

[0032] Figure 3 It is a schematic diagram of the structure of the provided transparent cover and hollow ring.

[0033] Numbers in the figure: 1 non-transparent bottom box, 2 transparent cover, 3 non-transparent cover, 4 first round opening, 5 second round opening, 6 third round opening, 7 common transparent transfer tube, 8 gauze, 9 arc-shaped snap-in protrusion, 10 common round opening plug, 11 hollow circular ring, 12 arc-shaped snap-in groove. DETAILED DESCRIPTION

[0034] The utility model is further described below in conjunction with the accompanying drawings and implementation modes.

[0035] like Figure 1-3 As shown, the utility model provides a device for propagating larval parasitic wasps, including a non-transparent bottom box 1, a transparent cover 2, and a non-transparent cover 3, which are arranged in sequence from bottom to top and can be sealed and connected in sequence. The non-transparent bottom box 1 is open, and a first inlet connected to its internal chamber is provided on the side wall of the non-transparent bottom box 1. A second inlet corresponding to the internal chamber is provided on the transparent cover 2 so that when the transparent cover 2 is covered on the opening of the non-transparent bottom box 1, the second inlet is connected to the internal chamber. The non-transparent cover 3 has a third inlet corresponding to the second inlet, and also includes a first plug capable of blocking the first inlet and a first transparent adapter capable of detachably connecting to the first inlet, and also includes a second plug capable of blocking the second inlet and a second transparent adapter capable of passing through the third inlet and detachably connecting to the second inlet.

[0036] In a specific implementation manner, Figure 1-3As shown, the first inlet is a first circular opening 4, the second inlet is a second circular opening 5, the third inlet is a third circular opening 6, the first transparent adapter is a first transparent adapter tube, the second transparent adapter is a second transparent adapter tube, the first transparent adapter tube and the second transparent adapter tube are a common transparent adapter tube 7, the two opposite ends of the common transparent adapter tube 7 are respectively a first end and a second end, the first end is used to connect to the first circular opening 4, and the second end is used to connect to the second circular opening 5, the first circular opening 4 is directly formed on the side wall, the first end is in a circular shape matching the first circular opening 4, and a rubber ring is arranged on its outer cover for Sealed and connected to the first circular opening 4, a through hole is provided on the transparent cover 2, and a hollow circular ring 11 protruding upward is provided at the edge of the through hole, and the through hole and the internal channel of the hollow circular ring 11 form the second circular opening 5, and two opposite arc-shaped clamping protrusions 9 are provided on the outer tube wall of the second end, and two opposite arc-shaped clamping grooves 12 corresponding to the two arc-shaped clamping protrusions 9 are provided on the inner side wall of the hollow circular ring 11, so that the two arc-shaped clamping protrusions 9 at the second end can be inserted into the second circular opening 5 and can be rotatably clamped to the two arc-shaped clamping grooves 12, and the third circular opening 6 of the non-transparent cover 3 can be sleeved on the outer peripheral side of the hollow circular ring 11.

[0037] The device, when in use, comprises the following:

[0038] ① Put a certain amount of hosts of appropriate age and an appropriate amount of host feed into the inner chamber of the non-transparent bottom box 1, put a cotton ball soaked in honey water, buckle the transparent cover 2 on the non-transparent bottom box 1, and buckle the second round opening 5 on the transparent cover 2 with the second round opening plug;

[0039] ② Remove the first round mouth plug at the first round mouth 4 on the side wall of the non-transparent bottom box 1, connect the first transparent adapter tube, introduce the parasitic wasp into the non-transparent bottom box 1 (using the light-repelling and upward-moving properties of the parasitic wasp), and finally block the first round mouth 4 with the first round mouth plug;

[0040] ③ The non-transparent bottom box 1 of step ② is placed in an insect breeding room under certain conditions, and the parasitic wasps complete the parasitic process in the box;

[0041] ④ After the parasitic process is completed, the non-transparent cover 3 is buckled on the transparent cover 2;

[0042] ⑤ Remove the second round-mouth plug and connect the second transparent transfer tube, use the phototaxis and upward attraction of the parasitic wasp to lead it into the transfer tube, and then lead it out of the device.

[0043] The above steps ① to ⑤ complete a parasitic process, thereby circulating the entire parasitic process to achieve the purpose of rapid expansion of parasitic wasps.

[0044] In a specific implementation manner, the lower end of the hollow ring 11 is detachably connected to the transparent cover 2 by bonding.

[0045] like Figure 1 As shown, the shared transparent transfer tube 7 is a curved tube.

[0046] In a specific implementation manner, the first circular opening 4 and the second circular opening 5 have the same size.

[0047] Wherein, the first plug is a first round-mouth plug, and the second plug is a second round-mouth plug.

[0048] In a specific implementation manner, the first round-mouth plug and the second round-mouth plug are a common round-mouth plug 10 .

[0049] like Figure 2 As shown, in a specific embodiment, the middle portion of the transparent cover 2 is a square opening and the square opening is covered with a gauze 8 with a mesh size of 60-100.

[0050] In a specific implementation manner, the non-transparent bottom box 1, the transparent cover 2, and the non-transparent cover 3 are sequentially connected by means of snap connections.

[0051] The dimensions of the non-transparent bottom box 1 are: 20-60 cm in length, 15-50 cm in width, and 4-10 cm in height.

[0052] Related settings for expansion using this device:

[0053] (1) Rearing environment: The following rearing steps and processes were all carried out in an insectary room with a light cycle set at 16 h light: 8 h dark, a temperature of 26 ± 1 °C, and a humidity of 45 ± 5%.

[0054] (2) Host rearing: After the moth eggs are hatched in the rearing box, they are fed with artificial feed. When they develop to the 2nd to 3rd age, they can be used as hosts of appropriate age for parasitism by parasitic wasps.

[0055] (3) Rearing of parasitic bees: When parasitic bees do not need to be parasitized, they are kept in a parasitic bee rearing box. The cotton balls soaked in 10% honey water are replaced once a day to provide a source of nutrition for the adult parasitic bees. When parasitic bees need to be parasitized, the cocoons that are about to emerge are placed in a "transfer tube" and wait for them to emerge. A cotton ball soaked in 10% honey water is placed in the tube.

[0056] (4) Parasitism: Place a certain amount of noctuid larvae hosts in a non-transparent bottom box 1, provide corresponding host feed, cover with a transparent cover 2, use a first transparent transfer tube to introduce the parasitic wasps in the tube into the non-transparent bottom box 1 through the first round hole 4, plug the first round hole and place it on the shelf of the insect breeding room to start parasitism.

[0057] (5) Leading out the parasitic wasps after the parasitism is over: buckle the non-transparent cover 3 onto the transparent cover 2, remove the second round port plug on the transparent cover 2, connect the second round port 5 with the second adapter transparent tube, and lead the parasitic wasps into the second transparent adapter tube by taking advantage of phototropism and upward attraction.

[0058] (6) Feeding of the host after parasitism: The noctuid larvae in the non-transparent bottom box 1 are transferred to a 36-hole pigment box for single-head feeding. An appropriate amount of feed is placed in each hole, and the lid of the 36-hole pigment box is closed.

[0059] (7) Collecting cocoons by parasitic bees: On the 10th to 12th day after the host is parasitized, open the lid of the 36-hole paint box, take out the cocoons from the holes, and collect them in the cocoon collection container.

[0060] Example 1: Parasitism of Spodoptera litura A by Parasitoides manilaensis

[0061] (1) Insect source: The parasitic wasp was Pleuronectinus manilaensis and the host was the larvae of Spodoptera litura.

[0062] (2) Parasitic container: The dimensions of the non-transparent bottom box 1 are 35 cm long, 25 cm wide and 5 cm high; the dimensions of the square opening of the transparent cover 2 are 25 cm long and 18 cm wide, and an 80-mesh gauze 8 is adhered to the square opening for ventilation.

[0063] (3) Operation process:

[0064] ① Put 300 3rd instar larvae of Spodoptera litura into a non-transparent bottom box 1, and put in an appropriate amount of block-shaped artificial feed for Spodoptera litura, put in two absorbent cotton balls soaked in 10% honey water, buckle the transparent cover 2 on the non-transparent bottom box 1, and plug the second round opening 5 on the transparent cover 2.

[0065] ② Transfer 30 pairs of Manila side groove braconids that have emerged for 3 days from the parasitic bee breeding box to the first transparent transfer tube of the parasitism device, and use the first transparent transfer tube to introduce the parasitic bees into the non-transparent bottom box 1. After the transfer is completed, plug the first round mouth 4 to start the first day of parasitism, and parasitize for 24 hours.

[0066] ③ After the first day of parasitism, the non-transparent cover 3 is buckled on the transparent cover 2, the second round port plug on the second round port 4 of the transparent cover 2 is removed and the second transparent transfer tube is connected, and all 30 pairs of parasitic wasps are introduced into the second transparent transfer tube.

[0067] ④ After the parasitism is over, the larvae of Spodoptera litura are transferred to a 36-hole paint box for single-head and single-hole breeding. An appropriate amount of artificial feed for Spodoptera litura is placed in each hole, and then the lid of the paint box is closed.

[0068] ⑤ On the 10th to 12th day after the host was parasitized, open the lid of the 36-hole paint box, take out the cocoons from the holes, collect them in the cocoon collection container, and count the host deaths and parasitism of the noctuid larvae and the cocoon collection of the parasitic bees.

[0069] Host mortality rate = number of dead hosts / total number of hosts

[0070] Parasitism: the number of noctuid larvae parasitized

[0071] Parasitism rate = number of parasites / total number of hosts

[0072] Cocoon yield: the number of parasitic wasp cocoons formed after the moth larvae are parasitized

[0073] Cocoon collection rate = cocoon collection amount / total host amount

[0074] ⑥ The parasitism operation on the second day was the same as that on the first day. The transfer tube into which 30 pairs of parasitic wasps were introduced in step ③ was reconnected to a new parasitism device to start the parasitism on the second day.

[0075] Under this experimental condition, parasitism continued until the third day.

[0076] The process of this example was repeated 5 times, and data processing and analysis were finally performed.

[0077] Control 1: Parasitoids of Manila parasitoids on Spodoptera litura A

[0078] (1) Test insect source: The parasitic wasp was Pleuronectinus manilaensis and the host was Spodoptera litura.

[0079] (2) Parasitic container: The parasitic box 1 is a plastic square box with a length of 36 cm, a width of 24 cm, and a height of 10 cm. A square opening with a length of 18 cm and a width of 12 cm is opened in the middle of the lid, and an 80-mesh gauze is adhered to the square opening for ventilation.

[0080] (3) Operation process:

[0081] ① Put 300 Spodoptera moth larvae and an appropriate amount of artificial feed for Spodoptera moth larvae into the parasitic box 1, then put two absorbent cotton balls soaked in 10% honey water, and close the parasitic box 1.

[0082] ② Transfer 30 pairs of 3-day-old Manila side groove braconid wasps from the parasitic bee breeding box to the parasitic box 1. The transfer method is to use a brush to pick up a single parasitic bee and introduce it into the box through the round opening on the side of the parasitic box 1. Finally, plug the round opening with a cotton ball to start the first day of parasitism, which lasts for 24 hours.

[0083] ③ After the parasitism is completed on the first day, the parasitism box 1 is placed in a 40*40*40cm insect cage, the lid is opened to allow 30 pairs of parasitic wasps to fly into the insect cage, and then the parasitism container is taken out from the insect cage.

[0084] ④ After the parasitism is completed, the Spodoptera litura larvae are raised in groups in the parasitism box 1, and artificial feed is added quantitatively every day.

[0085] ⑤ On the 10th to 12th day after the host was parasitized, the cocoons were taken out from the box and collected in the cocoon collection container. The number of host deaths and parasitism of noctuid larvae and the number of cocoons collected by parasitic bees were counted.

[0086] Host mortality rate = number of dead hosts / total number of hosts

[0087] Parasitism: the number of noctuid larvae parasitized

[0088] Parasitism rate = number of parasites / total number of hosts

[0089] Cocoon yield: the number of parasitic wasp cocoons formed after the moth larvae are parasitized

[0090] Cocoon collection rate = cocoon collection amount / total host amount

[0091] ⑥ The parasitism operation on the second day was the same as that on the first day. The 30 pairs of parasitoid wasps in the insect cage in step ③ were introduced into the new parasitism box 1 again using a writing brush to start the parasitism on the second day.

[0092] Under this experimental condition, parasitism continued until the 3rd day. The control treatment was repeated 5 times, and finally data processing and analysis were performed.

[0093] From the experimental results of control 1 and embodiment 1, it can be seen that: (1) the parasitic rate of the parasitic wasp propagation method in embodiment 1 is higher than that in control 1; (2) the cocoon recovery rate of the parasitic wasp propagation method in embodiment 1 is much higher than that in control 1, because the host is reared individually in the propagation method, which reduces the amount of disease susceptible to Spodoptera litura larvae during group rearing, and eliminates the disadvantage of mutual cannibalism of Spodoptera litura larvae during group rearing; (3) the working hours used in the parasitic wasp propagation method in embodiment 1 are about 1 / 2 of that in the control 1 method, which greatly reduces the labor cost.

[0094] Table 1 Comparison of the propagation effects of Pseudostomella manilaense

[0095]

[0096] Example 2: Parasitism of Spodoptera litura B by Parasitoides manilaensis

[0097] (1) Insect source: The parasitic wasp was Pleuronectinus manilaensis and the host was the larvae of Spodoptera litura.

[0098] (2) Parasitic container: The dimensions of the non-transparent bottom box 1 are 42 cm long, 30 cm wide and 7 cm high; the dimensions of the transparent cover square opening are 28 cm long and 20 cm wide, and a 100-mesh gauze is adhered to the square opening for ventilation.

[0099] (3) Operation process:

[0100] ① Place 450 3rd instar larvae of Spodoptera litura in the bottom box of the non-transparent bottom box 1, and put in an appropriate amount of block-shaped artificial feed for Spodoptera litura, and put in two absorbent cotton balls soaked in 10% honey water, buckle the transparent cover on the bottom box, and plug the second round opening of the transparent cover.

[0101] ② Transfer 30 pairs of Manila side groove braconids that have emerged for 3 days from the parasitic bee breeding box to the first transparent transfer tube of the non-transparent bottom box 1, and use the first transparent transfer tube to introduce the parasitic bees into the non-transparent bottom box 1. After the transfer is completed, plug the first round opening 4 on the side of the non-transparent bottom box 1 to start the first day of parasitism, which lasts for 24 hours.

[0102] ③ After the first day of parasitism, the non-transparent cover 3 is buckled on the transparent cover 2, the plug on the second round opening 5 is removed and the second transparent adapter tube is connected, and all 30 pairs of parasitic wasps are introduced into the second adapter tube.

[0103] ④ After the parasitism is over, the larvae of Spodoptera litura are transferred to a 36-hole paint box for single-head and single-hole breeding. An appropriate amount of artificial feed for Spodoptera litura is placed in each hole, and then the lid of the paint box is closed.

[0104] ⑤ On the 10th to 12th day after the host was parasitized, open the lid of the 36-hole paint box, take out the cocoons from the holes, collect them in the cocoon collection container, and count the host deaths and parasitism of the noctuid larvae and the cocoon collection of the parasitic bees.

[0105] Host mortality rate = number of dead hosts / total number of hosts

[0106] Parasitism: the number of noctuid larvae parasitized

[0107] Parasitism rate = number of parasites / total number of hosts

[0108] Cocoon yield: the number of parasitic wasp cocoons formed after the moth larvae are parasitized

[0109] Cocoon collection rate = cocoon collection amount / total host amount

[0110] ⑥ The parasitism operation on the second day was the same as that on the first day. The transfer tube into which 30 pairs of parasitic wasps were introduced in step ③ was reconnected to a new parasitism device to start the parasitism on the second day.

[0111] Under this experimental condition, parasitism continued until the third day.

[0112] The process of this example was repeated 3 times, and data processing and analysis were finally performed.

[0113] Control 2: Parasitoids of Manila parasitoids on Spodoptera litura B

[0114] (1) Test insect source: The parasitic wasp was Pleuronectinus manilaensis and the host was Spodoptera litura.

[0115] (2) Parasitic container: The parasitic box 2 is a plastic square box with a length of 42 cm, a width of 30 cm, and a height of 14 cm. A square opening with a length of 25 cm and a width of 18 cm is opened in the middle of the lid, and a 100-mesh gauze is attached to the square opening for ventilation.

[0116] (3) Operation process:

[0117] ① Place 450 noctuid larvae and an appropriate amount of artificial feed for noctuid larvae in the parasitic box 2, and then place two absorbent cotton balls soaked in 10% honey water, and then close the parasitic box 2.

[0118] ② Transfer 30 pairs of 3-day-old Manila side groove braconid wasps from the parasitic bee breeding box to the parasitic box 2. The transfer method is to use a brush to pick up a single parasitic bee and introduce it into the box through the round opening on the side of the parasitic box 2. Finally, plug the round opening with a cotton ball to start the first day of parasitism, which lasts for 24 hours.

[0119] ③ After the parasitism on the first day was completed, the parasitism box 2 was placed in a 60*60*60cm insect cage, the lid was opened to allow 30 pairs of parasitic wasps to fly into the insect cage, and then the parasitism container was taken out from the insect cage.

[0120] ④ After the parasitism is over, the Spodoptera litura larvae are raised in groups in the parasitism box 2, and artificial feed is added quantitatively every day.

[0121] ⑤ On the 10th to 12th day after the host was parasitized, the cocoons were taken out from the box and collected in the cocoon collection container. The number of host deaths and parasitism of noctuid larvae and the number of cocoons collected by parasitic bees were counted.

[0122] Host mortality rate = number of dead hosts / total number of hosts

[0123] Parasitism: the number of noctuid larvae parasitized

[0124] Parasitism rate = number of parasites / total number of hosts

[0125] Cocoon yield: the number of parasitic wasp cocoons formed after the moth larvae are parasitized

[0126] Cocoon collection rate = cocoon collection amount / total host amount

[0127] ⑥ The parasitism operation on the second day was the same as that on the first day. The 30 pairs of parasitoid wasps in the insect cage in step ③ were introduced into the new parasitism box 2 again through a writing brush to start the parasitism on the second day.

[0128] Under this experimental condition, parasitism continued until the third day. The control treatment was repeated three times, and finally data processing and analysis were performed.

[0129] Under the experimental conditions that the volume of the parasitic device is enlarged, the number of hosts is enlarged, and the number of parasitic bees remains unchanged, it can be seen from the experimental results of Control 2 and Example 2 that: (1) the parasitism rate and cocoon collection rate in Example 2 are higher than those in Control 2; (2) the working hours used in the parasitic bee propagation method in Example 2 are about 39% of those in Control 2, which greatly reduces the labor cost.

[0130] Table 2 Comparison of the propagation effects of Manila pleuronectin

[0131]

[0132]

[0133] Example 3: Parasitic Mythimna separasitized by Dinocampus punctatus

[0134] (1) Test insect source: The parasitic wasp was Mysops punctatus and the host was the larvae of the sticky insect.

[0135] (2) Parasitic container: The dimensions of the non-transparent bottom box 1 are 35 cm long, 25 cm wide and 5 cm high; the dimensions of the square opening of the transparent cover 2 are 25 cm long and 18 cm wide, and an 80-mesh gauze 8 is adhered to the square opening for ventilation.

[0136] (3) Operation process:

[0137] ① Because the mole-shaped braconid wasp is a parthenogenetic strain, place the cocoons of 30 mole-shaped braconid wasps that are about to emerge in the first transparent transfer tube in advance, and put a cotton ball soaked in 10% honey water into it.

[0138] ② Put 300 3rd instar armyworm larvae into the non-transparent bottom box 1, and put in an appropriate amount of block-shaped artificial feed for noctuids, and put in two absorbent cotton balls soaked in 10% honey water, buckle the transparent cover 2 on the bottom box, and plug the second round opening 5 on the transparent cover 2.

[0139] ③ Connect 30 mole-shaped braconid wasps that have emerged for 3 days to the first round opening 4 on the side of the non-transparent bottom box 1 through the first transparent transfer tube, and introduce them into the non-transparent bottom box 1. After the transfer, plug the first round opening 4 to start the first day of parasitism, which lasts for 24 hours.

[0140] ④ After the first day of parasitism, the non-transparent cover 3 is buckled on the transparent cover 2, the plug on the second round opening 5 of the transparent cover is removed and the second transparent adapter tube is connected, and all 30 parasitic wasps are introduced into the second transparent adapter tube.

[0141] ⑤ After the parasitism is over, transfer the larvae of the sticky insect to a 36-hole paint box for single-head and single-hole breeding. Place an appropriate amount of artificial feed for the sticky insect in each hole, and then close the lid of the paint box.

[0142] ⑥ On the 10th to 12th day after the host was parasitized, open the lid of the 36-hole paint box, take out the cocoons from the holes, collect them in the cocoon collection container, and count the host deaths and parasitism of armyworm larvae and the cocoon collection of parasitic bees.

[0143] Host mortality rate = number of dead hosts / total number of hosts

[0144] Parasitism: the number of noctuid larvae parasitized

[0145] Parasitism rate = number of parasites / total number of hosts

[0146] Cocoon yield: the number of parasitic wasp cocoons formed after the moth larvae are parasitized

[0147] Cocoon collection rate = cocoon collection amount / total host amount

[0148] ⑦ The parasitism operation on the second day was the same as that on the first day. The adapter tube into which the 30 parasitoid wasps were introduced in step ④ was reconnected to a new parasitism device to start the parasitism on the second day.

[0149] Under this experimental condition, the parasitism was continued until the 3rd day. The treatment of this example was repeated 5 times, and finally the data was processed and analyzed.

[0150] Control 3: Parasitoids of Myxomyces pilosa

[0151] (1) Test insect source: The parasitic wasp was Mysops punctatus and the host was Mythida separata.

[0152] (2) Parasitic container: The parasitic box 1 is a plastic square box with a length of 36 cm, a width of 24 cm, and a height of 10 cm. A square opening with a length of 18 cm and a width of 12 cm is opened in the middle of the lid, and an 80-mesh gauze is adhered to the square opening for ventilation.

[0153] (3) Operation process:

[0154] ① Put 300 noctuid larvae and an appropriate amount of artificial feed for armyworm larvae into the parasitic box 1, and then put two absorbent cotton balls soaked in 10% honey water, and then close the parasitic box 1.

[0155] ② Transfer 30 3-day-old Mylochromis japonicus from the parasitic bee breeding box to the parasitic box 1. The transfer method is to use a brush to pick up a single parasitic bee and introduce it into the box through the round opening on the side of the parasitic box 1. Finally, plug the round opening with a cotton ball to start the first day of parasitism, which lasts for 24 hours.

[0156] ③ After the parasitism is completed on the first day, the parasitism box 1 is placed in a 40*40*40cm insect cage, the lid is opened to allow 30 parasitic wasps to fly into the insect cage, and then the parasitism container is taken out from the insect cage.

[0157] ④ After the parasitism is completed, the armyworm larvae are raised in groups in the parasitism box 1, and artificial feed is added quantitatively every day.

[0158] ⑤ On the 10th to 12th day after the host was parasitized, the cocoons were taken out of the box and collected in the cocoon collection container. The host death and parasitism of the armyworm larvae and the cocoon collection of the parasitic bees were counted.

[0159] Host mortality rate = number of dead hosts / total number of hosts

[0160] Parasitism: the number of noctuid larvae parasitized

[0161] Parasitism rate = number of parasites / total number of hosts

[0162] Cocoon yield: the number of parasitic wasp cocoons formed after the moth larvae are parasitized

[0163] Cocoon collection rate = cocoon collection amount / total host amount

[0164] ⑥ The parasitism operation on the second day was the same as that on the first day. The 30 parasitoid wasps in the insect cage in step ③ were introduced into the new parasitism box 1 again through a writing brush to start the parasitism on the second day.

[0165] Under this experimental condition, parasitism continued until the 3rd day. The control treatment was repeated 5 times, and finally data processing and analysis were performed.

[0166] From the experimental results of Control 3 and Example 3, it can be seen that: (1) the parasitism rate of the method for expanding the breeding of the spotted mole hanging bracone in Example 3 is higher than that of Control 3; (2) the cocoon recovery rate of the method for expanding the breeding of the parasitic bee in Example 3 is higher than that of Control 3, because the host is raised individually in the expansion method, which reduces the amount of disease susceptibility of the sticky insect larvae during the group rearing process, and at the same time, it also avoids the problem of sticky insect larvae killing each other and bee cocoons being eaten during the group rearing process; (3) The number of working hours used in the method for expanding the breeding of the spotted mole hanging bracone in Example 3 is about 45% of that of the method in Control 3, which significantly reduces the labor cost.

[0167] Table 3 Comparison of the propagation effect of Cercospora punctata

[0168]

[0169] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A device for propagating larval parasitic wasps, characterized in that: The invention comprises a non-transparent bottom box, a transparent cover and a non-transparent cover which are arranged in sequence from bottom to top and can be sealed and connected in sequence. The non-transparent bottom box is arranged with an opening, a first inlet connected to its internal chamber is arranged on the side wall of the non-transparent bottom box, a second inlet corresponding to the internal chamber is arranged on the transparent cover so that when the transparent cover is arranged on the opening of the non-transparent bottom box, the second inlet is connected to the internal chamber, a third inlet corresponding to the second inlet is arranged on the non-transparent cover, and the invention also comprises a first plug which can block the first inlet and a first transparent adapter which can be detachably connected to the first inlet, a second plug which can block the second inlet and a second transparent adapter which can pass through the third inlet and can be detachably connected to the second inlet, and the middle part of the transparent cover is an open square opening and the square opening is covered with a gauze with a mesh number of 60-100.

2. The device for propagating larval parasitic wasps according to claim 1, characterized in that: The first inlet is a first circular port, the second inlet is a second circular port, the third inlet is a third circular port, the first transparent adapter is a first transparent adapter tube, the second transparent adapter is a second transparent adapter tube, the first transparent adapter tube and the second transparent adapter tube are a common transparent adapter tube, the two opposite ends of the common transparent adapter tube are a first end and a second end, the first end is used to connect with the first circular port, and the second end is used to connect with the second circular port, the first circular port is directly formed on the side wall, the first end is in a circular port shape matching the first circular port, and a The rubber ring is used to seal and connect to the first circular opening. A through hole is provided on the transparent cover. A hollow circular ring protruding upward is provided at the edge of the through hole. The through hole and the internal channel of the hollow circular ring form the second circular opening. Two opposite arc-shaped clamping protrusions are provided on the outer tube wall of the second end. Two opposite arc-shaped clamping grooves corresponding to the two arc-shaped clamping protrusions are provided on the inner side wall of the hollow circular ring, so that the two arc-shaped clamping protrusions of the second end can be inserted into the second circular opening and can be rotatably clamped to the two arc-shaped clamping grooves. The third circular opening of the non-transparent cover can be sleeved on the outer peripheral side of the hollow circular ring.

3. The device for propagating larval parasitic wasps according to claim 2, characterized in that: The lower end of the hollow circular ring is detachably connected to the transparent cover by bonding.

4. The device for propagating larval parasitic wasps according to claim 2, characterized in that: The shared transparent transfer tube is in the form of a curved tube.

5. The device for propagating larval parasitic wasps according to claim 2, characterized in that: The first circular opening and the second circular opening have the same size.

6. The device for propagating larval parasitic wasps according to claim 5, characterized in that: The first plug is a first round-mouth plug, and the second plug is a second round-mouth plug.

7. The device for propagating larval parasitic wasps according to claim 6, characterized in that: The first round-mouth plug and the second round-mouth plug are a common round-mouth plug.

8. The device for propagating larval parasitic wasps according to claim 1, characterized in that: The non-transparent bottom box, the transparent cover and the non-transparent cover are connected in sequence by means of snap connections.

9. The device for propagating larval parasitic wasps according to claim 1, characterized in that: The dimensions of the non-transparent bottom box are: 20-60 cm in length, 15-50 cm in width, and 4-10 cm in height.

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