Intermittent hypoxia chamber in animal experiments to simulate the detection of coronary atherosclerosis

Abstract

The utility model relates to an intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis, and belongs to the technical field of animal experiment hypoxia chambers, including a hypoxia chamber, and a sampling device is fixedly installed on one side of the hypoxia chamber. The intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis is provided with a sampling device on one side of the hypoxia chamber. During the experiment, the end caps at both ends of the sampling tube are closed, so that the hypoxia chamber remains sealed. When sampling detection is required, the end cap inside the hypoxia chamber is opened by an electric push rod, and the animal enters the sampling tube under the lure of food in the food trough, and then the end cap inside the hypoxia chamber is closed by an electric push rod, and the end cap outside the hypoxia chamber is opened by a staff member, and the sampled animal is taken out for detection, which solves the problem that the intermittent hypoxia chamber needs to be sealed, so it is inconvenient to open the intermittent hypoxia chamber during the experiment to take out the experimental animal for detection.

Description

Technical Field

[0001] The utility model relates to the technical field of animal experimental hypoxia chambers, in particular to an animal experimental intermittent hypoxia chamber for simulating and detecting coronary atherosclerosis. Background Art

[0002] Coronary atherosclerosis refers to the occurrence of atherosclerosis in the coronary arteries. It is the most common disease of the coronary arteries and a serious disease that threatens human health. Its cause is the result of the combined effects of multiple risk factors, common factors include genetic susceptibility, hypertension, hyperlipidemia, hyperglycemia, etc. These factors may damage blood vessels and accelerate the development of atherosclerosis.

[0003] In order to study coronary atherosclerosis, an animal experiment simulating the detection of coronary atherosclerosis was carried out in an intermittent hypoxia chamber. Since CIH exposure can lead to the occurrence of coronary atherosclerosis and pulmonary vascular remodeling, the pathological changes of the coronary and pulmonary arteries can be observed by replicating the OSA mouse model, and the body's inflammation and oxidative stress responses can be detected. The molecular mechanisms of HIF-1, β3AR / i NOS signaling pathways, Erk1 / 2 / ET-1 pathways, and l ncRNAMRPL20-AS1, miR-664a-3P, HIF-1, transforming growth factor-β (TGF-β) and caspase-3 molecules in the occurrence of coronary atherosclerosis and pulmonary vascular remodeling were explored.

[0004] However, in common animal experiment intermittent hypoxia chambers, the animals need to be placed in the chambers during the experiment, and the chambers need to be sealed. Therefore, it is not convenient to open the chambers during the experiment to take out the experimental animals for testing. In view of this, the present application proposes an intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides an intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis, which has the advantages of being convenient for sampling and testing experimental animals during the experiment, and solves the problem that the intermittent hypoxia chamber needs to be sealed, so it is inconvenient to open the intermittent hypoxia chamber during the experiment to take out the experimental animals for testing.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis, comprising a hypoxia chamber, a sampling device is fixedly installed on one side of the hypoxia chamber, and one side of the sampling device extends to the interior of the hypoxia chamber;

[0007] The sampling device includes a sampling tube that is passed through one side of the low oxygen bin, two mounting ear plates are fixedly installed on the top of both ends of the sampling tube, a rotating ear plate is rotatably installed between the two mounting ear plates, an end cover is fixedly installed on one side of the rotating ear plate, a sealing plug is fixedly installed on one side of the end cover, and a food attractant groove is fixedly installed inside the sampling tube.

[0008] Furthermore, a control panel is fixedly installed on the inner wall of the hypoxic chamber away from the sampling tube, two upper ear plates are fixedly installed on the bottom of the control panel, and an upper rotating seat is rotatably installed between the two upper ear plates.

[0009] Furthermore, two lower ear plates are fixedly mounted on the outer side of the end cover located inside the hypoxic bin, and a lower rotating seat is rotatably mounted between the two lower ear plates.

[0010] Furthermore, an electric push rod is fixedly installed on the bottom of the upper rotating seat, and a movable end of the electric push rod is fixedly connected to the top of the lower rotating seat.

[0011] Furthermore, an oxygen inlet is fixedly installed on one side of the hypoxic warehouse, a nitrogen inlet is fixedly installed on one side of the hypoxic warehouse, an exhaust port is fixedly installed on one side of the hypoxic warehouse, and a lighting lamp is fixedly installed on the inner top wall of the hypoxic warehouse.

[0012] Furthermore, feet are fixedly installed at the four corners of the bottom of the hypoxic bin, and two mounting blocks are fixedly installed at the top of the hypoxic bin near the front. Through holes are provided on one side of the two feet and the mounting blocks near the front of the hypoxic bin.

[0013] Furthermore, a door is provided on the front of the hypoxia chamber, and four positioning screws are fixedly installed on one side of the door. The positioning screws pass through the surface of the through holes and are threadedly installed with compression nuts.

[0014] Furthermore, the warehouse door includes a door panel, a sealing pad fixedly installed on one side of the door panel, and a sealing liner fixedly installed on one side of the sealing pad, the sealing liner contacts the inner wall of the hypoxia warehouse, and the positioning screw is fixedly installed on one side of the door panel.

[0015] Compared with the prior art, the utility model provides an intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis, which has the following beneficial effects:

[0016] The intermittent hypoxia chamber for animal experiments simulating the detection of coronary atherosclerosis has a sampling device arranged on one side of the hypoxia chamber. During the experiment, the end caps at both ends of the sampling tube are closed to keep the hypoxia chamber sealed. When sampling and testing is required, the end cap inside the hypoxia chamber is opened by an electric push rod, and the animal enters the sampling tube under the lure of food in the trough. Then the end cap inside the hypoxia chamber is closed by the electric push rod, and the staff opens the end cap outside the hypoxia chamber and takes out the sampled animal for testing, which solves the problem that the intermittent hypoxia chamber needs to be sealed, so it is inconvenient to open the intermittent hypoxia chamber during the experiment to take out the experimental animal for testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the structure of the utility model;

[0018] Figure 2 It is a side sectional view of the utility model;

[0019] Figure 3 It is a three-dimensional schematic diagram of the sampling device of the utility model.

[0020] In the figure: 1. hypoxia chamber; 2. sampling device; 21. sampling tube; 211. attractant trough; 22. mounting ear plate; 23. rotating ear plate; 24. end cover; 25. sealing plug; 3. control panel; 4. upper ear plate; 5. upper rotating seat; 6. lower ear plate; 7. lower rotating seat; 8. electric push rod; 9. oxygen inlet; 10. nitrogen inlet; 11. exhaust port; 12. lighting lamp; 13. base; 14. mounting block; 15. through hole; 16. chamber door; 161. door panel; 162. sealing pressure pad; 163. sealing liner; 17. positioning screw; 18. tightening nut. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1-3 An intermittent hypoxia chamber for animal experiments simulating the detection of coronary atherosclerosis includes a hypoxia chamber 1, a sampling device 2 is fixedly installed on one side of the hypoxia chamber 1, and one side of the sampling device 2 extends to the interior of the hypoxia chamber 1.

[0023] Among them, the sampling device 2 includes a sampling tube 21 inserted into one side of the low oxygen chamber 1, and two mounting ear plates 22 are fixedly installed on the top of both ends of the sampling tube 21, a rotating ear plate 23 is rotatably installed between the two mounting ear plates 22, an end cover 24 is fixedly installed on one side of the rotating ear plate 23, and a sealing plug 25 is fixedly installed on one side of the end cover 24, and a food attractant groove 211 is fixedly installed inside the sampling tube 21.

[0024] Place food in the attractant trough 211, open the end cover 24 inside the hypoxia chamber 1, and at this time, close the end cover 24 outside the hypoxia chamber 1 to keep the hypoxia chamber 1 sealed. When the animal enters the sampling tube 21, close the end cover 24 inside the hypoxia chamber 1, and then open the end cover 24 outside the hypoxia chamber 1 to take the animal out. During the process, the hypoxia chamber 1 is always in a sealed state and does not affect the experiment.

[0025] At the same time, a control panel 3 is fixedly installed on the inner wall of the hypoxic chamber 1 away from the sampling tube 21 , two upper ear panels 4 are fixedly installed on the bottom of the control panel 3 , and an upper rotating seat 5 is rotatably installed between the two upper ear panels 4 .

[0026] Among them, two lower ear plates 6 are fixedly installed on the outer side of the end cover 24 located inside the hypoxic chamber 1, and a lower rotating seat 7 is rotatably installed between the two lower ear plates 6.

[0027] Secondly, an electric push rod 8 is fixedly installed at the bottom of the upper rotating seat 5, and the movable end of the electric push rod 8 is fixedly connected to the top of the lower rotating seat 7. The end cover 24 inside the hypoxia bin 1 is driven by the electric push rod 8 to open and close.

[0028] At the same time, an oxygen inlet 9 is fixedly installed on one side of the hypoxic warehouse 1, a nitrogen inlet 10 is fixedly installed on one side of the hypoxic warehouse 1, an exhaust port 11 is fixedly installed on one side of the hypoxic warehouse 1, and a lighting lamp 12 is fixedly installed on the inner top wall of the hypoxic warehouse 1.

[0029] The four corners of the bottom of the hypoxic bin 1 are fixedly mounted with feet 13, and the top of the hypoxic bin 1 near the front is fixedly mounted with two mounting blocks 14. Through holes 15 are provided on one side of the two feet 13 and the mounting blocks 14 near the front of the hypoxic bin 1.

[0030] A door 16 is provided on the front of the hypoxia chamber 1 , and four positioning screws 17 are fixedly installed on one side of the door 16 . The positioning screws 17 pass through the surface of the through hole 15 and are threadedly installed with a locking nut 18 .

[0031] Secondly, the warehouse door 16 includes a door panel 161, a sealing pressure pad 162 fixedly installed on one side of the door panel 161, and a sealing liner 163 fixedly installed on one side of the sealing pressure pad 162. The sealing liner 163 contacts the inner wall of the hypoxic warehouse 1, and the positioning screw 17 is fixedly installed on one side of the door panel 161.

[0032] When closing the door 16 , the positioning screw 17 is passed through the through hole 15 , and then the door 16 is pressed against the front of the hypoxia chamber 1 by tightening the clamping nut 18 , and the sealing effect of the hypoxia chamber 1 is maintained by the sealing pressure pad 162 and the sealing liner 163 .

[0033] When this embodiment is in use, a sampling device 2 is provided on one side of the hypoxia chamber 1. When sampling inspection is required, one of the animals is taken out without affecting the sealing of the hypoxia chamber 1 and maintaining the oxygen concentration in the hypoxia chamber 1.

[0034] The electrical components appearing in the article are all electrically connected to the main controller and the power supply. The main controller can be a conventional known device for control such as a computer, and the existing public power connection technology is not described in detail in the article.

[0035] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0036] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intermittent hypoxia chamber for animal experiments simulating the detection of coronary atherosclerosis, comprising a hypoxia chamber (1), characterized in that: A sampling device (2) is fixedly mounted on one side of the hypoxia bin (1), and one side of the sampling device (2) extends into the interior of the hypoxia bin (1); The sampling device (2) comprises a sampling tube (21) which is passed through one side of the hypoxia chamber (1), two mounting ear plates (22) are fixedly mounted on the tops of both ends of the sampling tube (21), a rotating ear plate (23) is rotatably mounted between the two mounting ear plates (22), an end cover (24) is fixedly mounted on one side of the rotating ear plate (23), a sealing plug (25) is fixedly mounted on one side of the end cover (24), and a food attractant groove (211) is fixedly mounted inside the sampling tube (21).

2. The intermittent hypoxia chamber for animal experiments for simulating detection of coronary atherosclerosis according to claim 1, characterized in that: A control panel (3) is fixedly mounted on the inner side wall of the hypoxic chamber (1) away from the sampling tube (21), two upper ear panels (4) are fixedly mounted on the bottom of the control panel (3), and an upper rotating seat (5) is rotatably mounted between the two upper ear panels (4).

3. The intermittent hypoxia chamber for animal experiments for simulating detection of coronary atherosclerosis according to claim 2, characterized in that: Two lower ear plates (6) are fixedly mounted on the outer side of the end cover (24) located inside the hypoxic bin (1), and a lower rotating seat (7) is rotatably mounted between the two lower ear plates (6).

4. The intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis according to claim 3, characterized in that: An electric push rod (8) is fixedly mounted on the bottom of the upper rotating seat (5), and a movable end of the electric push rod (8) is fixedly connected to the top of the lower rotating seat (7).

5. The intermittent hypoxia chamber for animal experiments for simulating detection of coronary atherosclerosis according to claim 1, characterized in that: An oxygen inlet (9) is fixedly installed on one side of the hypoxic chamber (1), a nitrogen inlet (10) is fixedly installed on one side of the hypoxic chamber (1), an exhaust port (11) is fixedly installed on one side of the hypoxic chamber (1), and an illumination lamp (12) is fixedly installed on the inner top wall of the hypoxic chamber (1).

6. The intermittent hypoxia chamber for animal experiments for simulating detection of coronary atherosclerosis according to claim 1, characterized in that: The four corners of the bottom of the hypoxic bin (1) are fixedly mounted with feet (13), and the top of the hypoxic bin (1) near the front is fixedly mounted with two mounting blocks (14), and one side of the two feet (13) and mounting blocks (14) near the front of the hypoxic bin (1) is provided with through holes (15).

7. The intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis according to claim 6, characterized in that: The front of the hypoxia chamber (1) is provided with a chamber door (16), and one side of the chamber door (16) is fixedly mounted with four positioning screws (17), and the positioning screws (17) are threadedly mounted with compression nuts (18) through the surfaces of the through holes (15).

8. The intermittent hypoxia chamber for animal experiments for simulating the detection of coronary atherosclerosis according to claim 7, characterized in that: The chamber door (16) comprises a door panel (161), a sealing pressure pad (162) fixedly mounted on one side of the door panel (161), and a sealing liner (163) fixedly mounted on one side of the sealing pressure pad (162); the sealing liner (163) contacts the inner side wall of the hypoxia chamber (1); and a positioning screw (17) is fixedly mounted on one side of the door panel (161).

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