Absorbance detection module and bacteriophage display device with same

Abstract

The utility model provides an absorbance detection module and a bacteriophage display device with the same, the absorbance detection module comprises a module main body, a first accommodating hole and a second accommodating hole are formed on the module main body, a first cuvette is arranged in the first accommodating hole, and a second cuvette is arranged in the second accommodating hole; the light source plate is arranged on the first side face of the module body, and a first light source and a second light source which are independent are arranged on the side, close to the first side face, of the light source plate. The detection plate is arranged on the second side surface of the module main body, a first optical sensor and a second optical sensor which are mutually independent are arranged on one side, close to the second side surface, of the detection plate, and the first side surface and the second side surface are two opposite sides of the module main body; the first cuvette is located on a light path between the first light source and the first light sensor, and the second cuvette is located on a light path between the second light source and the second light sensor. According to the utility model, the integration level of the module is higher, the size of the module can be obviously reduced, the occupation of the internal space of the bacteriophage display device can be reduced, and the miniaturization design of the bacteriophage display device structure is facilitated.

Description

Technical Field

[0001] The utility model belongs to the technical field of phage display equipment design, and in particular relates to an absorbance detection module and a phage display device having the same. Background Art

[0002] Phage is a type of tiny life form that infects and replicates inside bacteria. Using synthetic biology tools, the genetic information of phage can be reprogrammed, making phage or infected bacteria an important tool for drug screening, protein optimization, and biomarker screening.

[0003] Phage display technology is one of the two major development platforms for all approved antibody drugs in the world. Due to its mature research system, low cost, and simple operation, phage display technology has attracted widespread attention from researchers and has extremely important commercial value.

[0004] In the field of antibody preparation, phage display technology provides a simple and fast genetic engineering antibody preparation route for fully human recombinant antibodies: a complete set of antibody heavy chain variable region (VH) and light chain variable region (VL) genes are amplified from human immune cells using PCR technology and expressed on the phage surface in the form of fusion proteins.

[0005] Various types of antibodies can be screened through phage display technology. Compared with monoclonal antibodies produced by immunizing animals, monoclonal antibodies produced using this technology have the advantages of higher efficiency and faster production.

[0006] The current phage display technology is implemented by a combination of manual operations using equipment such as a full-temperature vibration incubator, a centrifuge, and a cloning and panning device. The aforementioned equipment is a single-function, independent device that requires operator participation, which leads to the risk of cross-contamination during the culture and panning process. Manual participation also leads to manual selection bias, reducing the stability of the process and results. In addition, the independent application of single-function modules and the low degree of equipment integration lead to time-consuming and high-cost phage screening and display. Based on the aforementioned deficiencies in the prior art, there is an urgent need for a phage display device with a high degree of integration and automation. Based on the aforementioned purpose, the utility model proposes an absorbance detection module used in the phage display device. Utility Model Content

[0007] Therefore, the technical problem to be solved by the present invention is to provide an absorbance detection module and a phage display device having the same, which can reduce the occupation of the internal space of the phage display device, thereby facilitating the miniaturization design of the phage display device structure.

[0008] In order to solve the above problems, the utility model provides an absorbance detection module, comprising:

[0009] A module body, wherein a first accommodating hole and a second accommodating hole are formed on the module body, wherein the first accommodating hole is provided with a first cuvette for containing a sample solution of a first concentration, and the second accommodating hole is provided with a second cuvette for containing a sample solution of a second concentration;

[0010] A light source board is disposed on the first side surface of the module body, and a first light source and a second light source that are independent of each other are disposed on a side of the light source board close to the first side surface;

[0011] A detection board is arranged on the second side surface of the module body, and a first light sensor and a second light sensor which are independent of each other are arranged on a side of the detection board close to the second side surface, and the first side surface and the second side surface are two opposite sides of the module body;

[0012] The first cuvette is located on an optical path between the first light source and the first light sensor, and the second cuvette is located on an optical path between the second light source and the second light sensor.

[0013] In some embodiments, a first waste liquid pipe joint is provided at the bottom of the first cuvette, and the first waste liquid pipe joint is controllably connected to a waste liquid pool; a second waste liquid pipe joint is provided at the bottom of the second cuvette, and the second waste liquid pipe joint is controllably connected to the waste liquid pool.

[0014] In some embodiments, the first cuvette and the second cuvette both include a quartz glass tube and a plastic carrier sealed at the bottom of the glass tube, and the first waste liquid pipe connector and the second waste liquid pipe connector are respectively connected to the corresponding carrier.

[0015] In some embodiments, a sealing gasket is clamped between the glass tube and the carrier, a cuvette pressing plate is provided on the end surface of the top wall of the glass tube, and the cuvette pressing plate is detachably connected to the module body; and / or,

[0016] The top of the glass cylinder is in a trumpet shape that is larger at the top and smaller at the bottom.

[0017] In some embodiments, a needle washing structure is also formed on the module body, and the needle washing structure includes a needle washing pool and a waste liquid buffer chamber. The needle washing pool is a blind hole with an opening facing upward. When the aspiration needle is controlled to be inserted into the blind hole during the needle washing process, the waste liquid overflowing from the opening can be collected by the waste liquid buffer chamber.

[0018] In some embodiments, the needle washing pool and the module body are an integrated structure, and a liquid passage cavity is constructed on the module body, the liquid passage cavity is arranged around the needle washing pool, and a cache shell is provided in the lower area of ​​the liquid passage cavity, the cache shell is connected with the liquid passage cavity and the waste liquid cache cavity is formed therebetween; and / or, the central axes of the needle washing pool, the first cuvette and the second cuvette are on the same straight line.

[0019] In some embodiments, a third waste liquid pipe connector is provided at the bottom of the cache housing, and the third waste liquid pipe connector is controllably connected to the waste liquid pool.

[0020] In some embodiments, the first waste liquid pipe connector, the second waste liquid pipe connector, and the third waste liquid pipe connector are simultaneously connected to the inlet of the waste liquid pump.

[0021] In some embodiments, the liquid passage chamber has a top opening, and the top opening is covered with a cover plate corresponding to the opening position of the needle washing pool.

[0022] The utility model also provides a phage display device, comprising the above absorbance detection module.

[0023] The utility model provides an absorbance detection module and a phage display device having the same. A first cuvette and a second cuvette are integrated on a module body at the same time, and two light sources and two light sensors are respectively arranged corresponding to the first cuvette and the second cuvette, so as to realize the detection of the absorbance of two sample liquids with different OD values ​​in one module. The module structure is simple and compact in design. Compared with two separate detection modules, the module in the utility model has a higher degree of integration, can significantly reduce the volume of the module, and further can reduce the occupation of the internal space of the phage display device, thereby facilitating the miniaturized design of the structure of the phage display device. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the three-dimensional structure of the absorbance detection module of an embodiment of the utility model;

[0025] Figure 2 for Figure 1 A top view of

[0026] Figure 3 for Figure 2 Sectional view of II.

[0027] The reference numerals are:

[0028] 11. Module body; 112. First accommodating hole; 113. Second accommodating hole; 114. First cuvette; 1141. First waste liquid pipe joint; 1151. Second waste liquid pipe joint; 115. Second cuvette; 116. Glass cylinder; 117. Supporting member; 118. Cuvette pressing plate; 119. Liquid passage chamber; 12. Light source board; 13. Detection board; 141. Needle washing pool; 142. Waste liquid buffer chamber; 143. Buffer shell; 1431. Third waste liquid pipe joint; 15. Cover plate. DETAILED DESCRIPTION

[0029] See also Figures 1 to 3 As shown, according to an embodiment of the utility model, an absorbance detection module is provided, comprising:

[0030] A module body 11, wherein a first accommodating hole 112 and a second accommodating hole 113 are formed on the module body 11, wherein the first accommodating hole 112 is provided with a first cuvette 114 for containing a sample solution of a first concentration, and the second accommodating hole 113 is provided with a second cuvette 115 for containing a sample solution of a second concentration, wherein the OD values ​​of the sample solution of the first concentration and the sample solution of the second concentration are different, so as to meet the requirements of the phage display process for sample solutions of different OD values;

[0031] The light source board 12 is arranged on the first side surface of the module body 11, and the light source board 12 is provided with a first light source (not shown in the figure) and a second light source (not shown in the figure) which are independent of each other on the side close to the first side surface, that is, the first light source and the second light source are both arranged on the inner side surface of the light source board 12. It can be understood that the light source board 12 should also include corresponding cables to form an electrical connection with the corresponding power supply. These related structures are not shown in the figure. In a specific embodiment, the first light source can be, for example, an LED lamp biased towards infrared light, and the second light source can be, for example, an LED lamp biased towards ultraviolet light. Of course, according to the detection needs of the absorbance of the sample liquid, the wavelengths of the light emitted by the first light source and the second light source can be reasonably selected;

[0032] The detection board 13 is arranged on the second side surface of the module body 11, and a first light sensor (not shown in the figure) and a second light sensor (not shown in the figure) which are independent of each other are provided on the side of the detection board 13 close to the second side surface. The first side surface and the second side surface are opposite sides of the module body 11, that is, the first light sensor and the second light sensor are both located on the inner side of the detection board 13. It can be understood that the detection board 13 is also configured with a corresponding light intensity detection circuit and corresponding electronic components, which are well known in the industry and will not be elaborated in the present utility model.

[0033] The first cuvette 114 is located on the optical path between the first light source and the first light sensor, and the second cuvette 115 is located on the optical path between the second light source and the second light sensor, that is, the light of the first wavelength emitted by the first light source passes through the first sample liquid in the first cuvette 114 and is detected by the first light sensor arranged opposite thereto, and the light of the second wavelength emitted by the second light source passes through the second sample liquid in the second cuvette 115 and is detected by the second light sensor arranged opposite thereto, thereby realizing the absorbance detection of the corresponding sample liquid. It can be understood that the absorbance detection result can reflect the OD value of the sample liquid.

[0034] In this technical solution, a first cuvette 114 and a second cuvette 115 are integrated on the module body 11 at the same time, and two light sources and two light sensors are respectively arranged corresponding to the first cuvette 114 and the second cuvette 115, so as to realize the detection of the absorbance of two sample liquids with different OD values ​​in one module. The module structure is simple and compact in design. Compared with two separate detection modules, the module in the utility model has a higher degree of integration, which can significantly reduce the volume of the module, and further reduce the occupation of the internal space of the phage display device, thereby facilitating the miniaturization design of the structure of the phage display device.

[0035] In some embodiments, a first waste liquid pipe joint 1141 is provided at the bottom of the first cuvette 114, and the first waste liquid pipe joint 1141 is controllably connected to a waste liquid pool (not shown in the figure); a second waste liquid pipe joint 1151 is provided at the bottom of the second cuvette 115, and the second waste liquid pipe joint 1151 is controllably connected to the waste liquid pool. In this technical solution, the waste liquid pipe joint 1151 and the corresponding pipeline are connected to the waste liquid pool, so that the sample liquid in the corresponding cuvette can be discharged to the waste liquid pool for storage after the corresponding absorbance detection is completed, and the cuvette can be cleaned, and the cleaned waste liquid is also discharged to the waste liquid pool for storage, so that the processing efficiency is higher.

[0036] In a specific embodiment, the first cuvette 114 and the second cuvette 115 both include a glass tube 116 made of quartz and a supporting member 117 made of plastic and sealed at the bottom of the glass tube 116. It can be understood that the glass tube 116 is a cylinder that passes through from top to bottom, and the first waste liquid pipe joint 1141 and the second waste liquid pipe joint 1151 are respectively connected to the corresponding supporting member 117. By designing the cuvette as a glass tube 116 with a quartz material on the upper part and a supporting member 117 with a plastic material on the lower part, the difficulty of connecting the corresponding waste liquid pipe joint and the cuvette can be reduced.

[0037] In another preferred embodiment, a sealing gasket (not shown in the figure) is clamped between the glass tube 116 and the supporting member 117, and a cuvette pressing plate 118 is provided on the end face of the top wall of the glass tube 116. The cuvette pressing plate 118 and the module body 11 are detachably connected. The aforementioned detachable connection can, for example, be threadedly connected between bolts on both sides of the corresponding cuvette and the top surface of the module body 11. The cuvette pressing plate 118 can apply an axial downward pressure to the glass tube 116 so that the glass tube 116 can form a reliable clamping of the sealing gasket with the supporting member 117, thereby ensuring the sealing between the glass tube 116 and the supporting member 117.

[0038] In order to facilitate the injection of the sample liquid into the corresponding glass tube 116 , the top of the glass tube 116 is in the shape of a trumpet with a larger top and a smaller bottom.

[0039] In some preferred embodiments, see Figure 3 As shown, a needle washing structure (not marked in the figure) is also formed on the module body 11, and the needle washing structure includes a needle washing pool 141 and a waste liquid buffer chamber 142. The needle washing pool 141 is a blind hole with an opening facing upward. When the aspiration needle is controlled to be inserted into the blind hole during needle washing, the waste liquid overflowing from the opening can be collected by the waste liquid buffer chamber 142. In a specific embodiment, the needle washing structure is located in the area between the two cuvettes, so that the cleaning of the cuvettes on both sides after the sample liquid is added can be achieved more efficiently.

[0040] In this technical solution, the needle washing pool 141 is a blind hole. When the pipette needle is inserted into the blind hole and the cleaning liquid is sprayed, the sprayed cleaning liquid will collide with the bottom wall of the blind hole and overflow upward along the vertical wall of the blind hole. The overflow process can clean the outer wall of the pipette needle, and the overflowed cleaning liquid is stored and collected in the waste liquid buffer chamber 142.

[0041] In some embodiments, the needle washing pool 141 and the module body 11 are an integrated structure, and a liquid passage cavity 119 is constructed on the module body 11. The liquid passage cavity 119 is arranged at intervals around the needle washing pool 141, and a cache shell 143 is provided in the lower area of ​​the liquid passage cavity 119. The cache shell 143 is connected to the liquid passage cavity 119 and forms the waste liquid cache cavity 142 therebetween. In this technical solution, the cache shell 143 and the module body 11 are in an assembly relationship. For example, the cache shell 143 can be threadedly connected to the module body 11 to form the aforementioned waste liquid cache cavity 142. Such a design can reduce the structural design.

[0042] In some embodiments, the central axes of the needle washing pool 141, the first cuvette 114 and the second cuvette 115 are on the same straight line. With this arrangement, the lateral movement of the pipette needle thereon can be controlled by a linear guide rail parallel to the aforementioned straight line to achieve corresponding adjustment of the pipette needle and each cuvette and the needle washing pool 141, and the displacement control structure design of the pipette needle is simplified.

[0043] A third waste liquid pipe joint 1431 is provided at the bottom of the buffer shell 143, and the third waste liquid pipe joint 1431 is controllably connected to the waste liquid pool to prevent excessive overflow of waste liquid.

[0044] In another preferred embodiment, the first waste liquid pipe connector 1141, the second waste liquid pipe connector 1151 and the third waste liquid pipe connector 1431 are simultaneously connected to the inlet of the waste liquid pump (not shown in the figure). In this technical solution, one waste liquid pump can be used to pump and discharge waste liquid at the three waste liquid pipe positions at the same time, which simplifies the pipeline design and reduces the design and manufacturing costs of the device.

[0045] In some embodiments, the liquid passage chamber 119 has a top opening, and the top opening is covered with a cover plate 15 corresponding to the opening position of the needle washing pool 141. The cover plate 15 can be detachably assembled and connected to the top surface of the module body 11 by bolts, which can prevent waste liquid from splashing to the outside during the needle washing process.

[0046] According to an embodiment of the utility model, a phage display device is also provided, comprising the above absorbance detection module.

[0047] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0048] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred implementations of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. An absorbance detection module, characterized in that: include: A module body (11), wherein a first accommodating hole (112) and a second accommodating hole (113) are formed on the module body (11), wherein the first accommodating hole (112) is provided with a first cuvette (114) for containing a sample solution of a first concentration, and the second accommodating hole (113) is provided with a second cuvette (115) for containing a sample solution of a second concentration; A light source board (12) is arranged on a first side surface of the module body (11), and a first light source and a second light source that are independent of each other are arranged on a side of the light source board (12) close to the first side surface; a detection board (13) disposed on a second side surface of the module body (11), and a first light sensor and a second light sensor independent of each other are disposed on a side of the detection board (13) close to the second side surface, the first side surface and the second side surface being opposite sides of the module body (11); The first cuvette (114) is located on an optical path between the first light source and the first light sensor, and the second cuvette (115) is located on an optical path between the second light source and the second light sensor.

2. The absorbance detection module according to claim 1, characterized in that: A first waste liquid pipe joint (1141) is provided at the bottom of the first cuvette (114), and the first waste liquid pipe joint (1141) is controllably connected to a waste liquid pool; a second waste liquid pipe joint (1151) is provided at the bottom of the second cuvette (115), and the second waste liquid pipe joint (1151) is controllably connected to the waste liquid pool.

3. The absorbance detection module according to claim 2, characterized in that: The first cuvette (114) and the second cuvette (115) both include a glass tube (116) made of quartz material and a supporting member (117) made of plastic material and sealedly connected to the bottom of the glass tube (116); the first waste liquid pipe connector (1141) and the second waste liquid pipe connector (1151) are respectively connected to the corresponding supporting member (117).

4. The absorbance detection module according to claim 3, characterized in that: A sealing gasket is clamped between the glass cylinder (116) and the carrier (117), a cuvette pressing plate (118) is provided on the end surface of the top wall of the glass cylinder (116), and the cuvette pressing plate (118) is detachably connected to the module body (11); and / or, The top of the glass cylinder (116) is in the shape of a trumpet mouth that is larger at the top and smaller at the bottom.

5. The absorbance detection module according to claim 2, characterized in that: A needle washing structure is also formed on the module body (11), and the needle washing structure includes a needle washing pool (141) and a waste liquid buffer chamber (142). The needle washing pool (141) is a blind hole with an opening facing upward. When the aspiration needle is controlled to be inserted into the blind hole during the needle washing process, the waste liquid overflowing from the opening can be collected by the waste liquid buffer chamber (142).

6. The absorbance detection module according to claim 5, characterized in that: The needle washing pool (141) and the module body (11) are an integrated structure, and a liquid passage cavity (119) is constructed on the module body (11), and the liquid passage cavity (119) is arranged around the needle washing pool (141) at intervals, and a cache shell (143) is provided in the lower area of ​​the liquid passage cavity (119), and the cache shell (143) is connected with the liquid passage cavity (119) to form the waste liquid cache cavity (142) therebetween; and / or, the central axes of the needle washing pool (141), the first cuvette (114) and the second cuvette (115) are on the same straight line.

7. The absorbance detection module according to claim 6, characterized in that: A third waste liquid pipe joint (1431) is provided at the bottom of the cache shell (143), and the third waste liquid pipe joint (1431) is controllably connected to the waste liquid pool.

8. The absorbance detection module according to claim 7, characterized in that: The first waste liquid pipe joint (1141), the second waste liquid pipe joint (1151) and the third waste liquid pipe joint (1431) are simultaneously connected to the inlet of the waste liquid pump.

9. The absorbance detection module according to claim 6, characterized in that: The liquid passage chamber (119) has a top opening, and the top opening is covered with a cover plate (15) having a position corresponding to the opening of the needle washing pool (141).

10. A phage display device, characterized in that: The absorbance detection module comprises the absorbance detection module according to any one of claims 1 to 9.

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