Cell incubator sterilization station

Abstract

The utility model relates to the technical field of cell incubator sterilization, and discloses a cell incubator sterilization station which comprises a sterilization station body and a plurality of first supporting frames used for supporting cell incubators, a second supporting frame is installed at the bottom of the sterilization station body, and a static pressure box is installed at the top of the sterilization station body. A fresh air assembly and an exhaust assembly are installed at the top of the static pressure box, a front glass door and a plurality of butt joint openings are arranged on the sterilization station body, and glove openings are symmetrically formed in the front glass door. According to the cell incubator sterilization station, by arranging a plurality of butt-joint openings, glove openings and annular inflation sealing structures, sterilization of a single cell incubator or simultaneous sterilization of a plurality of cell incubators can be achieved, and opening and closing of sealing doors of the butt-joint openings and glass doors on the cell incubators can be achieved; the communication and isolation of the sterilization chamber of the cell incubator sterilization station main body and the inner cavity of the cell incubator are realized, the sterilization waiting time of a plurality of cell incubators can be shortened, and the production efficiency of the whole process is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture box sterilization, in particular to a cell culture box sterilization station. Background Art

[0002] Cell culture incubators play an important role in research and production in the fields of biology and medicine, as cell culture must be observed in as sterile and meticulous a manner as possible. They keep unwanted microorganisms away from the culture, thus providing ideal growth conditions. At present, the sterilization methods of cell culture incubators mainly include disinfectant wiping, ultraviolet light irradiation, formaldehyde fumigation, chlorine dioxide gas disinfection, and sterilization together with isolators. These methods have the disadvantages of poor repeatability, high destructiveness, complex operation, easy loss, sterilization dead corners, large consumption of manpower and time, and harm to the health of operators.

[0003] At present, vaporized hydrogen peroxide (VHP) sterilization technology is widely used and has obvious advantages in sterilization effectiveness, personnel safety, material compatibility and environmental friendliness. The application of VHP sterilization technology in isolators is relatively mature and can meet the legal and regulatory requirements of GMP. However, the current sterilization method using cell culture incubators and isolators docking still has the following shortcomings.

[0004] Currently, the common method of sterilizing by docking the cell culture incubator and the isolator is that only a single cell culture incubator can be sterilized at a time. When multiple cell culture incubators need to be sterilized, it will take a lot of time and affect the operation of other processes in the isolator. In addition, when the cell culture incubator and the isolator are sterilized by docking, the gas flow inside the isolator is slow, which will reduce the sterilization efficiency of the isolator. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a cell culture box sterilization station, which solves the problems mentioned in the above background.

[0006] The utility model provides the following technical solutions: a cell culture box sterilization station, comprising: a sterilization station body for sterilizing a culture box, having a sterilization chamber, at least two docking ports are provided on the side wall of the sterilization chamber, the docking ports are used to dock the culture box, each of the docking ports is provided with a sealing door, the sealing door is rotatably connected to the side wall of the sterilization chamber, and an annular inflatable sealing structure is provided at the edge of the docking port, the annular inflatable sealing structure is used to achieve sealing and fixing between the side wall of the sterilization chamber and the sealing door;

[0007] A static pressure box is arranged on the main body of the sterilization station, and the air intake and exhaust system of the static pressure box includes a fresh air component, an exhaust component, a circulating fan and a circulating high-efficiency filter. The fresh air component and the exhaust component are both installed on the static pressure box and communicated with the inner cavity of the static pressure box. The circulating high-efficiency filter and the circulating fan are installed on the bottom surface of the static pressure box in sequence from bottom to top, and the air inlet of the circulating high-efficiency filter is communicated with the inner cavity of the static pressure box, and the air outlet of the circulating high-efficiency filter is communicated with the sterilization chamber of the main body of the sterilization station;

[0008] A sterilization assembly, comprising a hydrogen peroxide evaporation plate and a hydrogen peroxide delivery device, wherein the hydrogen peroxide evaporation plate is disposed in the static pressure box, and a liquid outlet of the hydrogen peroxide delivery device is located above the hydrogen peroxide evaporation plate;

[0009] The control unit, the sterilization assembly, the air intake and exhaust system of the static pressure box, and the air pump connected to the annular inflatable sealing structure are all electrically connected to the control unit.

[0010] Preferably, the annular inflatable sealing structure includes a first annular inflatable sealing strip and a second annular inflatable sealing strip, the outer edge of the docking interface is provided with a first annular groove, the inner edge of the docking interface is provided with a second annular groove, the first annular inflatable sealing strip is arranged in the first annular groove, the second annular inflatable sealing strip is arranged in the second annular groove, the first annular inflatable sealing strip in the first annular groove is used for sealing between the side wall of the sterilization chamber and the incubator, and the second annular inflatable sealing strip in the second annular groove is used for sealing between the side wall of the sterilization chamber and the sealing door.

[0011] Preferably, the incubator comprises a box body and a door, wherein the door is arranged at an opening of the box body and is rotatably connected to the box body;

[0012] An annular sealing structure is provided at the opening of the box body, and the annular sealing structure cooperates with the first annular inflatable sealing strip in the first annular groove to achieve sealing between the side wall of the sterilization chamber and the incubator.

[0013] Preferably, the sterilization station body further comprises at least two first support frames, the first support frames are arranged in one-to-one correspondence with the docking ports, and the first support frames are used to support the incubator;

[0014] A guide rail is symmetrically arranged on the top of each of the first support frames, and a sliding component cooperating with the guide rail is arranged on the bottom of the incubator, and the guide rail is used to realize the movement and guidance of the incubator.

[0015] Preferably, the fresh air component includes a fresh air duct, a fresh air butterfly valve, a fresh air fan and a fresh air high efficiency filter, wherein the fresh air butterfly valve, the fresh air fan and the fresh air high efficiency filter are sequentially arranged on the fresh air duct from top to bottom and are all located inside the static pressure box;

[0016] The exhaust assembly includes an exhaust duct, an electric ball valve, an exhaust butterfly valve, a first catalytic decomposition filter, an exhaust high-efficiency filter and an exhaust fan. The first catalytic decomposition filter, the exhaust high-efficiency filter and the exhaust fan are arranged on the exhaust duct from top to bottom in sequence and are all located inside the static pressure box. The electric ball valve and the exhaust butterfly valve are arranged in parallel above the first catalytic decomposition filter.

[0017] Preferably, the hydrogen peroxide delivery device comprises a hydrogen peroxide storage container, a weighing device and a peristaltic pump, the hydrogen peroxide storage container is placed on the weighing device, the liquid inlet of the peristaltic pump is connected to the hydrogen peroxide storage container, the liquid outlet of the peristaltic pump is connected to the liquid outlet of the hydrogen peroxide delivery device, and the peristaltic pump is used to deliver the hydrogen peroxide liquid in the hydrogen peroxide storage container to the hydrogen peroxide evaporation plate.

[0018] Preferably, the sterilization assembly further comprises: a second catalytic decomposition filter, a vacuum pump, a first solenoid valve and a second solenoid valve;

[0019] The air suction port of the vacuum pump is communicated with the air vent of the incubator, and the air outlet of the vacuum pump is communicated with the fresh air duct of the fresh air component;

[0020] The second catalytic decomposition filter is disposed between the vacuum pump and the vent of the incubator, and the first solenoid valve is disposed between the second catalytic decomposition filter and the vent of the incubator.

[0021] Preferably, the sterilization station body further comprises a second support frame, and the second support frame is fixed below the sterilization chamber;

[0022] The hydrogen peroxide storage container, the peristaltic pump, the weighing device and the vacuum pump are all installed on the second supporting frame.

[0023] Preferably, the sterilization assembly further comprises an air inlet pipe, which is connected to an air vent of the incubator and is used to deliver compressed air to the incubator, and a third solenoid valve is provided on the air inlet pipe.

[0024] Preferably, it also includes: a differential pressure sensor, a hydrogen peroxide concentration sensor and a cantilever touch display screen;

[0025] The differential pressure sensor and the hydrogen peroxide concentration sensor are both arranged in the sterilization chamber, one side of the sterilization chamber is an operation side, the operation side is provided with a front glass door, an airtight gasket is installed on the front glass door, and a glove port is provided on the glass door, and the glove port is used to connect a sealing glove;

[0026] The cantilever touch display screen is installed on the second support frame of the sterilization station body and is located adjacent to the front glass door. The cantilever touch display screen, the pressure difference sensor and the hydrogen peroxide concentration sensor are all electrically connected to the control unit.

[0027] Compared with the prior art, the utility model has the following beneficial effects:

[0028] 1. The cell culture incubator sterilization station can realize the sterilization of a single cell culture incubator or multiple cell culture incubators at the same time by providing multiple docking ports, glove ports and annular inflatable sealing structures. The sealing door of the docking port and the glass door on the cell culture incubator are opened and closed to realize the connection and isolation between the sterilization chamber of the cell culture incubator sterilization station body and the internal cavity of the cell culture incubator, which can reduce the waiting time for sterilization of multiple cell culture incubators and improve the production efficiency of the entire process.

[0029] 2. The cell culture incubator sterilization station is provided with a hydrogen peroxide storage container, a peristaltic pump and a hydrogen peroxide evaporation disk. The peristaltic pump is started to transport a hydrogen peroxide solution through the hydrogen peroxide storage container, so that the hydrogen peroxide solution drips onto the hydrogen peroxide evaporation disk. The hydrogen peroxide evaporation disk is heated to convert the hydrogen peroxide liquid into steam, thereby generating hydrogen peroxide vapor, so that the inner cavity of the cell culture incubator sterilization station body and the inner cavity of the cell culture incubator are filled with hydrogen peroxide vapor, thereby achieving disinfection and sterilization.

[0030] 3. The cell culture incubator sterilization station is provided with a circulating fan and a circulating high-efficiency filter, which are started to drive air circulation, accelerate the process of the inner cavity of the hydrogen peroxide steam cell culture incubator sterilization station body and the internal cavity of the cell culture incubator, and achieve the effect of rapid sterilization. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a schematic diagram of the structure of a cell culture box sterilization station according to an embodiment of the utility model;

[0032] Figure 2 This is a schematic diagram of the structure of the cell culture box sterilization station and two cell culture boxes docking in an embodiment of the utility model;

[0033] Figure 3 This is a schematic diagram of the pipeline connections of the fresh air component, the exhaust component, and the sterilization component of the embodiment of the utility model;

[0034] Figure 4This is a schematic structural diagram of a cell culture box sterilization station docking with three cell culture boxes in another embodiment of the utility model;

[0035] Figure 5 This is a schematic diagram of the docking structure of a cell culture box sterilization station and multiple cell culture boxes according to another embodiment of the utility model.

[0036] In the figure: 1. incubator; 2. sterilization station body; 3. first support frame; 4. second support frame; 5. static pressure box; 6. fresh air assembly; 61. fresh air butterfly valve; 62. fresh air fan; 63. fresh air high efficiency filter; 7. exhaust assembly; 71. electric ball valve; 72. exhaust butterfly valve; 73. first catalytic decomposition filter; 74. exhaust high efficiency filter; 75. exhaust fan; 8. front glass door; 9. docking port; 10. glove port; 11. annular inflatable sealing structure; 111. first annular inflatable Air sealing strip; 112, second annular inflatable sealing strip; 12, sealing door; 131, circulating fan; 132, circulating high-efficiency filter; 141, hydrogen peroxide storage container; 142, peristaltic pump; 143, hydrogen peroxide evaporation disk; 144, second catalytic decomposition filter; 145, vacuum pump; 146, first solenoid valve; 147, second solenoid valve; 15, air sealing gasket; 16, air inlet pipe; 17, third solenoid valve; 18, guide rail; 19, cantilever touch display screen; 20, box door. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0038] See also Figure 1-3A cell culture box sterilization station comprises: a sterilization station body 2 for sterilizing a culture box 1, having a sterilization chamber, two docking ports 9 are provided on the side wall of the sterilization chamber, the docking ports 9 are used to dock the culture box 1, each docking port 9 is provided with a sealing door 12, the sealing door 12 is rotatably connected to the side wall of the sterilization chamber, and an annular inflatable sealing structure 11 is provided at the edge of the docking port 9, the annular inflatable sealing structure 11 is used to achieve sealing and fixing between the side wall of the sterilization chamber and the sealing door 12; static The static pressure box 5 is arranged on the sterilization station body 2. The air inlet and exhaust system of the static pressure box 5 includes a fresh air component 6, an exhaust component 7, a circulating fan 131 and a circulating high-efficiency filter 132. The fresh air component 6 and the exhaust component 7 are both installed on the static pressure box 5 and communicated with the inner cavity of the static pressure box 5. The circulating high-efficiency filter 132 and the circulating fan 131 are installed on the bottom surface of the static pressure box 5 from bottom to top, and the air inlet of the circulating high-efficiency filter 132 is communicated with the inner cavity of the static pressure box 5. The outlet of the circulating high-efficiency filter 132 The air outlet is connected to the sterilization chamber of the sterilization station body 2, and the circulating fan 131 and the circulating high-efficiency filter 132 are started to drive air circulation, accelerate the process of the inner cavity of the hydrogen peroxide steam cell culture box 1 sterilization station body 2 and the internal cavity of the cell culture box 1, and achieve the effect of rapid sterilization. The sterilization component includes a hydrogen peroxide evaporation disk 143 and a hydrogen peroxide delivery device. The hydrogen peroxide evaporation disk 143 is arranged in the static pressure box 5, and the position of the liquid outlet of the hydrogen peroxide delivery device is located above the hydrogen peroxide evaporation disk 143; the control unit, the sterilization component, the air intake and exhaust system of the static pressure box 5, and the inflation pump connected to the annular inflation sealing structure are all electrically connected to the control unit, which can realize the simultaneous sterilization of multiple cell culture boxes 1, and the sealing door 12 of the docking port 9 and the box door 20 on the cell culture box 1 are opened and closed to realize the connection and isolation of the sterilization chamber of the sterilization station body 2 of the cell culture box 1 and the internal cavity of the cell culture box 1, which can reduce the waiting time for sterilization of multiple culture boxes 1 and improve the production efficiency of the entire process.

[0039] The annular inflatable sealing structure 11 includes a first annular inflatable sealing strip 111 and a second annular inflatable sealing strip 112. A first annular groove is provided on the outer edge of the docking interface 9, and a second annular groove is provided on the inner edge of the docking interface 9. The first annular inflatable sealing strip 111 is provided in the first annular groove, and the second annular inflatable sealing strip 112 is provided in the second annular groove. The first annular inflatable sealing strip 111 in the first annular groove is used for sealing between the side wall of the sterilization chamber and the incubator 1, and the second annular inflatable sealing strip 112 in the second annular groove is used for sealing between the side wall of the sterilization chamber and the sealing door 12.

[0040] The incubator 1 includes a box body and a door 20. The door 20 is arranged at the opening of the box body and is rotatably connected to the box body. An annular sealing structure is arranged at the opening of the box body. The annular sealing structure cooperates with the first annular inflatable sealing strip 111 in the first annular groove to achieve sealing between the side wall of the sterilization chamber and the incubator 1.

[0041] The sterilization station body 2 also includes at least two first support frames 3, which are arranged one by one corresponding to the docking ports 9. The first support frames 3 are used to support the incubator 1. The top of each first support frame 3 is symmetrically provided with a guide rail 18, and the bottom of the incubator 1 is provided with a sliding component that cooperates with the guide rail 18. The guide rail 18 is used to realize the movement and guidance of the incubator 1.

[0042] The fresh air component 6 includes a fresh air duct, a fresh air butterfly valve 61, a fresh air fan 62 and a fresh air high-efficiency filter 63. The fresh air butterfly valve 61, the fresh air fan 62 and the fresh air high-efficiency filter 63 are arranged on the fresh air duct from top to bottom and are all located inside the static pressure box 5. The exhaust component 7 includes an exhaust duct, an electric ball valve 71, an exhaust butterfly valve 72, a first catalytic decomposition filter 73, an exhaust high-efficiency filter 74 and an exhaust fan 75. The first catalytic decomposition filter 73, the exhaust high-efficiency filter 74 and the exhaust fan 75 are arranged on the exhaust duct from top to bottom and are all located inside the static pressure box 5. The electric ball valve 71 and the exhaust butterfly valve 72 are arranged in parallel above the first catalytic decomposition filter 73.

[0043] The hydrogen peroxide delivery device includes a hydrogen peroxide storage container 141, a weighing device and a peristaltic pump 142. The hydrogen peroxide storage container 141 is placed on the weighing device. The liquid inlet of the peristaltic pump 142 is connected to the hydrogen peroxide storage container 141. The liquid outlet of the peristaltic pump 142 is connected to the liquid outlet of the hydrogen peroxide delivery device. The peristaltic pump 142 is used to deliver the hydrogen peroxide liquid in the hydrogen peroxide storage container 141 to the hydrogen peroxide evaporation disk 143. The peristaltic pump 142 is started to deliver the hydrogen peroxide solution in the hydrogen peroxide storage container 141, so that the hydrogen peroxide solution drips on the hydrogen peroxide evaporation disk 143. The hydrogen peroxide evaporation disk 143 is heated to turn the hydrogen peroxide liquid into steam, generating hydrogen peroxide steam, so that the inner cavity of the cell culture box 1 sterilization station body 2 and the inner cavity of the cell culture box 1 are filled with hydrogen peroxide steam to achieve disinfection and sterilization. The sterilization component also includes : The second catalytic decomposition filter 144, the vacuum pump 145, the first solenoid valve 146 and the second solenoid valve 147, the air suction port of the vacuum pump 145 is connected to the air vent of the incubator 1, the air outlet of the vacuum pump 145 is connected to the fresh air duct of the fresh air component 6, the second catalytic decomposition filter 144 is arranged between the vacuum pump 145 and the air vent of the incubator 1, the first solenoid valve 146 is arranged between the second catalytic decomposition filter 144 and the air vent of the incubator 1, the second catalytic decomposition filter 144, the vacuum pump 145, the first solenoid valve 146 and the second solenoid valve 147 are opened, and suction is generated by the vacuum pump 145 to extract gas from the cell culture boxes 1 on the left and right sides, and the gas is extracted between the fresh air butterfly valve 61 and the fresh air fan 62, so that the hydrogen peroxide vapor can fill the entire cell culture box 1 more quickly, thereby achieving the purpose of faster sterilization.

[0044] The sterilization station body 2 also includes a second support frame 4, which is fixed below the sterilization chamber. The hydrogen peroxide storage container 141, the peristaltic pump 142, the weighing device and the vacuum pump 145 are all installed on the second support frame 4. The sterilization component also includes an air inlet pipe 16, which is connected to the air vent of the incubator 1 for delivering compressed air to the incubator 1. A third solenoid valve 17 is arranged on the air inlet pipe 16. The air inlet of the cell culture box 1 is connected to the air inlet pipe 16, and the air inlet pipe 16 is arranged on the third solenoid valve 17. When the third solenoid valve 17 is opened, compressed air is injected into the cell culture box 1, which can accelerate the reduction of the concentration of hydrogen peroxide inside the cell culture box 1.

[0045] In a specific embodiment, the cell culture incubator sterilization station also includes: a differential pressure sensor, a hydrogen peroxide concentration sensor and a cantilever touch display screen 19. The differential pressure sensor and the hydrogen peroxide concentration sensor are both arranged in the sterilization chamber. One side of the sterilization chamber is the operation side. The operation side is provided with a front glass door 8. An air-sealing gasket 15 is installed on the front glass door 8. A glove port 10 is provided on the front glass door 8. The glove port 10 is used to connect a sealed glove. The cantilever touch display screen 19 is installed on the second support frame 4 of the sterilization station body 2 and is located adjacent to the front glass door 8. The cantilever touch display screen 19, the differential pressure sensor and the hydrogen peroxide concentration sensor are all electrically connected to the control unit.

[0046] See also Figure 2-3 In an embodiment where two cell culture boxes 1 need to be sterilized at the same time, when in use, the cell culture boxes 1 are placed on the first support frame 3, and slide on the guide rail 18 on the first support frame 3 to dock with the docking port 9 of the sterilization station body 2, the sealed doors 12 on both sides of the sterilization station body 2 are in an open state, the front door glass is in a closed state, and the box doors 20 on the cell culture boxes 1 on both sides are kept closed.

[0047] First, a leakage test is performed, and the first annular inflatable sealing strip 111, the second annular inflatable sealing strip 112 and the air sealing gasket 15 are all closed, and the fresh air butterfly valve 61, the exhaust butterfly valve 72, the electric ball valve 71, the second solenoid valve 147 and the remaining components are all closed, and a closed space is maintained in the sterilization station body 2. Under the control of the control unit, sterile compressed air is injected into the first pipeline injection port between the fresh air butterfly valve 61 and the fresh air fan 62, and after the pressure inside the sterilization station body 2 reaches the set value, the injection of compressed air is stopped, and a period of time is waited. If the pressure value detected by the pressure differential sensor inside the sterilization station body 2 is within the expected requirements, the leakage test is qualified and the next sterilization can be carried out.

[0048] After the leakage test is qualified, the door 20 of the cell culture box 1 on the left and right cell culture boxes 1 is opened with the gloves in the front door glass glove opening 10 for sterilization, the electric ball valve 71 is started, and the pressure in the sterilization station body 2 is maintained in a suitable range by controlling the opening size of the electric ball valve 71, and the peristaltic pump 142 is started to extract the hydrogen peroxide solution in the hydrogen peroxide storage container 141, and the hydrogen peroxide solution is transported to the position of the hydrogen peroxide evaporation disk 143 through the third pipeline, so that the hydrogen peroxide solution drips on the hydrogen peroxide evaporation disk 143, and the hydrogen peroxide evaporation disk 143 is evaporating. The hydrogen peroxide liquid is heated to become steam, generating hydrogen peroxide vapor, and the circulating fan 131 is controlled to start, so that the hydrogen peroxide vapor fills the entire sterilization station body 2 and the interior of the cell culture box 1. The second catalytic decomposition filter 144, the vacuum pump 145, the first solenoid valve 146 and the second solenoid valve 147 are opened, and suction is generated by the vacuum pump 145 to extract gas from the cell culture boxes 1 on the left and right sides, and the gas is extracted between the fresh air butterfly valve 61 and the fresh air fan 62, so that the hydrogen peroxide vapor can fill the entire cell culture box 1 more quickly, thereby achieving a faster sterilization purpose.

[0049] After the sterilization is completed, ventilation is performed, the peristaltic pump 142 is turned off, the heating of the hydrogen peroxide evaporation plate 143 is stopped, the fresh air butterfly valve 61 and the fresh air fan 62 are opened, the exhaust air butterfly valve 72 and the exhaust air fan 75 are opened, the electric ball valve 71, the second catalytic decomposition filter 144, the vacuum pump 145, the first solenoid valve 146 and the second solenoid valve 147 are closed, and the third solenoid valve 17 is opened to inject compressed air into the cell culture box 1 to accelerate the reduction of the concentration of hydrogen peroxide inside the cell culture box 1. When the hydrogen peroxide concentration sensor inside the sterilization station body 2 detects a hydrogen peroxide concentration of When the temperature is lower than the set value, the fresh air butterfly valve 61 and the fresh air fan 62 are closed, the exhaust butterfly valve 72, the exhaust fan 75 and the circulating fan 131 are closed, and then gloves are used on the front door glass to close the doors 20 on the cell culture boxes 1 on both sides, and the air seal on the cell culture box 1 is inflated to keep the cell culture box 1 in a sealed state, the first annular inflation sealing strip 111 and the second annular inflation sealing strip 112 are closed, and the sterilization is completed. At this time, the cell culture boxes 1 on both sides can slide on the guide rails 18 on the bracket of the cell culture box 1, and the cell culture box 1 can be transferred to other places for use.

[0050] When the sterilization station body 2 has multiple docking ports 9, if only one cell culture box 1 is sterilized, the cell culture box 1 is placed on one of the first support frames 3. Except for the docking port 9 corresponding to the position of the cell culture box 1, the second annular inflatable sealing strip 112 in the other docking ports 9 is kept open until the sterilization of the cell culture box 1 is completed. The others are consistent with the above. After the sterilization is completed, the cell culture box 1 is transferred to another place for use according to the above operation.

[0051] When multiple cell culture boxes 1 are not sterilized at the same time, in order to reduce the sterilization waiting time, when the sterilization of the cell culture box 1 is not completed, the above sterilization state is maintained, the other sealing doors 12 are kept closed, and the second annular inflatable sealing strip 112 is kept open from the start of the leakage test to the completion of the process on the cell culture box 1 being sterilized. The cell culture box 1 waiting to be sterilized is placed on any vacant first support frame 3, and slides on the guide rail 18 of the first support frame 3 to dock with the docking port 9 of the sterilization station body 2. The box door 20 on the cell culture box 1 is kept closed, and the first annular inflatable sealing strip 111 is opened to wait for the cell culture box 1 being sterilized. After the sterilization process of the cell culture box 1 is completed, the door 20 of the sterilized cell culture box 1 is closed with gloves, and then the sealing door 12 and the glass door are closed, and the second annular inflatable sealing strip 112 at the corresponding position is opened, and the second annular inflatable sealing strip 112 at the docking port 9 at the corresponding position of the sterilized cell culture box 1 is closed, and then the sealing door 12 is opened with gloves, and the glass door of the cell culture box 1 is opened. At this time, the sterilization station body 2 and the cell culture box 1 form a closed space, and the leakage test, sterilization and ventilation mode are turned on. After the sterilization is completed, the cell culture box 1 is transferred to another place for use according to the above operation, which can shorten the time for replacing the docking and reduce the waiting time for sterilization.

[0052] In a specific embodiment, the fresh air component 6, the exhaust component 7, the annular inflatable sealing structure 11, the sterilization component, the air sealing gasket 15 and the third solenoid valve 17 are controlled by a control unit.

[0053] In this embodiment, the cross section of the sterilization chamber is rectangular, and the docking ports 9 on the side wall are respectively located at the left and right ends. It should be noted that the shape structure of the sterilization chamber, the number and position of the docking ports 9 are only examples, not limitations, and those skilled in the art can set the shape of the sterilization chamber, the number and position of the docking ports 9 according to actual needs.

[0054] like Figure 4 As shown, a cell culture incubator sterilization station provided by another embodiment of the utility model has docking ports 9 on the three side walls of the sterilization chamber, which can be docked with three cell culture incubators 1 .

[0055] like Figure 5 As shown, a cell culture incubator sterilization station provided by another embodiment of the utility model has a cross-section of a sterilization station body (2) of a polygonal structure, a sterilization chamber having more side walls, and more docking ports 9 can be provided to dock with multiple cell culture incubators 1.

[0056] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture incubator sterilization station, characterized in that: include: A sterilization station body (2) for sterilizing an incubator (1), comprising a sterilization chamber, wherein at least two docking ports (9) are provided on the side wall of the sterilization chamber, wherein the docking ports (9) are used to dock the incubator (1), wherein each docking port (9) is provided with a sealing door (12), wherein the sealing door (12) is rotatably connected to the side wall of the sterilization chamber, and an annular inflatable sealing structure (11) is provided at the edge of the docking port (9), wherein the annular inflatable sealing structure (11) is used to achieve sealing and fixing between the side wall of the sterilization chamber and the sealing door (12); A static pressure box (5) is arranged on the sterilization station body (2); the air intake and exhaust system of the static pressure box (5) comprises a fresh air component (6), an exhaust component (7), a circulating fan (131) and a circulating high-efficiency filter (132); the fresh air component (6) and the exhaust component (7) are both installed on the static pressure box (5) and communicate with the inner cavity of the static pressure box (5); the circulating high-efficiency filter (132) and the circulating fan (131) are installed on the bottom surface of the static pressure box (5) in sequence from bottom to top; the air inlet of the circulating high-efficiency filter (132) is communicated with the inner cavity of the static pressure box (5); and the air outlet of the circulating high-efficiency filter (132) is communicated with the sterilization chamber of the sterilization station body (2); A sterilization component, comprising a hydrogen peroxide evaporation plate (143) and a hydrogen peroxide delivery device, wherein the hydrogen peroxide evaporation plate (143) is arranged in the static pressure box (5), and the liquid outlet of the hydrogen peroxide delivery device is located above the hydrogen peroxide evaporation plate (143); The control unit, the sterilization assembly, the air intake and exhaust system of the static pressure box (5) and the air pump connected to the annular air-filled sealing structure are all electrically connected to the control unit.

2. The cell culture incubator sterilization station according to claim 1, characterized in that: The annular inflatable sealing structure (11) comprises a first annular inflatable sealing strip (111) and a second annular inflatable sealing strip (112); the outer edge of the docking port (9) is provided with a first annular groove, and the inner edge of the docking port (9) is provided with a second annular groove; the first annular inflatable sealing strip (111) is arranged in the first annular groove, and the second annular inflatable sealing strip (112) is arranged in the second annular groove; the first annular inflatable sealing strip (111) in the first annular groove is used for sealing between the side wall of the sterilization chamber and the incubator (1), and the second annular inflatable sealing strip (112) in the second annular groove is used for sealing between the side wall of the sterilization chamber and the sealing door (12).

3. The cell culture incubator sterilization station according to claim 2, characterized in that: The incubator (1) comprises a box body and a box door (20), wherein the box door (20) is arranged at an opening of the box body and is rotatably connected to the box body; An annular sealing structure is provided at the opening of the box body, and the annular sealing structure cooperates with a first annular inflatable sealing strip (111) in the first annular groove to achieve sealing between the side wall of the sterilization chamber and the incubator (1).

4. The cell culture incubator sterilization station according to claim 1, characterized in that: The sterilization station body (2) further comprises at least two first support frames (3), wherein the first support frames (3) are arranged in one-to-one correspondence with the docking ports (9), and the first support frames (3) are used to support the incubator (1); A guide rail (18) is symmetrically arranged at the top of each of the first support frames (3), and a sliding component cooperating with the guide rail (18) is arranged at the bottom of the incubator (1), and the guide rail (18) is used to realize the movement and guidance of the incubator (1).

5. The cell culture incubator sterilization station according to claim 1, characterized in that: The fresh air component (6) comprises a fresh air duct, a fresh air butterfly valve (61), a fresh air fan (62) and a fresh air high-efficiency filter (63); the fresh air butterfly valve (61), the fresh air fan (62) and the fresh air high-efficiency filter (63) are arranged on the fresh air duct in sequence from top to bottom and are all located inside the static pressure box (5); The exhaust assembly (7) comprises an exhaust duct, an electric ball valve (71), an exhaust butterfly valve (72), a first catalytic decomposition filter (73), an exhaust high-efficiency filter (74) and an exhaust fan (75); the first catalytic decomposition filter (73), the exhaust high-efficiency filter (74) and the exhaust fan (75) are sequentially arranged on the exhaust duct from top to bottom and are all located inside the static pressure box (5); the electric ball valve (71) and the exhaust butterfly valve (72) are arranged in parallel above the first catalytic decomposition filter (73).

6. The cell culture incubator sterilization station according to claim 5, characterized in that: The hydrogen peroxide delivery device comprises a hydrogen peroxide storage container (141), a weighing device and a peristaltic pump (142); the hydrogen peroxide storage container (141) is placed on the weighing device; the liquid inlet of the peristaltic pump (142) is connected to the hydrogen peroxide storage container (141); the liquid outlet of the peristaltic pump (142) is connected to the liquid outlet of the hydrogen peroxide delivery device; the peristaltic pump (142) is used to deliver the hydrogen peroxide liquid in the hydrogen peroxide storage container (141) to the hydrogen peroxide evaporation plate (143).

7. The cell culture incubator sterilization station according to claim 6, characterized in that: The sterilization assembly further comprises: a second catalytic decomposition filter (144), a vacuum pump (145), a first solenoid valve (146) and a second solenoid valve (147); The air suction port of the vacuum pump (145) is in communication with the air vent of the incubator (1), and the air outlet of the vacuum pump (145) is in communication with the fresh air duct of the fresh air component (6); The second catalytic decomposition filter (144) is arranged between the vacuum pump (145) and the vent of the incubator (1), and the first solenoid valve (146) is arranged between the second catalytic decomposition filter (144) and the vent of the incubator (1).

8. The cell culture incubator sterilization station according to claim 7, characterized in that: The sterilization station body (2) further comprises a second support frame (4), wherein the second support frame (4) is fixed below the sterilization chamber; The hydrogen peroxide storage container (141), the peristaltic pump (142), the weighing device and the vacuum pump (145) are all mounted on the second support frame (4).

9. The cell culture incubator sterilization station according to claim 7, characterized in that: The sterilization assembly further comprises an air inlet pipe (16), the air inlet pipe (16) being connected to the air vent of the incubator (1) and being used for delivering compressed air to the incubator (1), and a third solenoid valve (17) being arranged on the air inlet pipe (16).

10. The cell culture incubator sterilization station according to claim 1, characterized in that: Also includes: A differential pressure sensor, a hydrogen peroxide concentration sensor, and a cantilever touch display screen (19); The differential pressure sensor and the hydrogen peroxide concentration sensor are both arranged in the sterilization chamber, one side of the sterilization chamber is an operation side, the operation side is provided with a front glass door (8), an airtight gasket (15) is installed on the front glass door (8), and a glove opening (10) is provided on the glass door (8), and the glove opening (10) is used to connect a sealing glove; The cantilever touch display screen (19) is installed on the second support frame (4) of the sterilization station body (2) and is located adjacent to the front glass door (8). The cantilever touch display screen (19), the pressure difference sensor and the hydrogen peroxide concentration sensor are all electrically connected to the control unit.

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