Platform used for non-invasive selective unilateral lung drug delivery of rat

Abstract

The platform comprises a base and a lower-layer platform body, a second connecting piece is arranged on the rear side face of the lower-layer platform body, a supporting rod is rotationally connected to the second connecting piece, the bottom end of the supporting rod is inserted between limiting frames, and the limiting frames are arranged on the base. An upper-layer platform is arranged on the front side face of the lower-layer platform. According to the unilateral drug delivery model, the drug can be stably, limitedly and repeatedly delivered to unilateral lung tissue; the distribution of drugs in unilateral lung tissues is observed with naked eyes by applying an Evan's blue dye, a unilateral lung injury model is manufactured by using lipopolysaccharide, the expression of caspase-1 and caspase-11 proteins is detected by applying immunohistochemistry in a left lung and a right lung, the expression of the caspase-1 and the caspase-11 proteins can be directly stimulated to be increased by combining the lipopolysaccharide, the expression of the caspase-11 is not found to be increased in the right lung, and the expression of the caspase-1 and the caspase-11 is not found to be increased in the left lung and the right lung. Multiple independent experiments prove that the device is stable, limited and repeatable.

Description

Technical Field

[0001] The utility model specifically relates to the technical field of rat experimental instruments, and specifically provides a platform for non-invasive selective unilateral pulmonary administration in rats. Background Technique

[0002] Acute respiratory distress syndrome (ARDS) is a clinically difficult and severe disease. The number of confirmed cases globally exceeds 3 million per year. As the disease progresses, the mortality rate gradually increases, and the mortality rate of each subtype grouping exceeds 30%. One of the reasons for the high mortality rate is that the current treatment method is mainly supportive treatment centered on respiratory support, without specific treatment methods. The reason for the lack of specific treatment for ARDS is its heterogeneity. Among them, morphological heterogeneity is divided into unilateral lung injury and bilateral lung injury according to the scope of lung injury. The comparison between the two is the key direction of future research proposed in the new global definition of ARDS in 2023. The results of the LIVE study, the first global study on individualized ventilation strategies for ARDS patients, show that appropriate individualized ventilation can reduce the mortality rate of patients, while inappropriate individualized ventilation can increase the mortality rate of patients. Inappropriate individualized intervention damages the rights and interests of patients. Therefore, corresponding research should first be observed at the animal level, that is, a unilateral lung injury model should be established.

[0003] Due to considerations such as the model-making method, mortality rate, and repeatability, the current unilateral lung injury models are mostly applied to large animals such as dogs and pigs, and the corresponding experimental conditions and experimental costs are relatively high. Rats, as the observation objects of most animal experiments, are widely recognized for their repeatability, easy availability, and genetic background stability. Since the anatomical structure of rats is that there is one lung lobe on the left and four lung lobes on the right, the rat unilateral lung injury model is mostly a left lung injury model. The existing methods include puncturing the trachea with sharp instruments such as syringe needles, invasively administering liquids into the lungs, or adjusting the body position after non-invasive tracheal intubation to make the left side lower so that the liquid diffuses by gravity. However, none of these methods can avoid the right side being contaminated by injury factors (such as hydrochloric acid, endotoxin, etc.). Through the self-made modeling platform, the present invention can stably, locally, and repeatedly administer drugs to the unilateral lung tissue (left side), and through subsequent experiments, it is verified that the right side has not directly contacted injury factors, laying a foundation for studying the corresponding mechanism. Content of the Utility Model

[0004] The purpose of the present utility model is to provide a platform for non-invasive selective unilateral pulmonary administration in rats, so as to solve the problems raised in the above background technique.

[0005] To achieve the above object, the present utility model provides the following technical solutions: A platform used for non-invasive selective unilateral pulmonary drug administration in rats, comprising a base and a lower platform. A second connecting member is provided on the rear side of the lower platform. A support rod is rotatably connected to the second connecting member, and the bottom end of the support rod is inserted between the limiting frames. An upper platform is provided on the front side of the lower platform, and the upper platform is connected to the lower platform by fixing screws. A magnetic attracting sheet is provided on the front side of the upper platform. Limiting rods are provided on the front side of the upper platform, and the limiting rods are respectively arranged on the left and right sides of the magnetic attracting sheet. A rubber band is provided on the limiting rods, and the rubber band is arranged on the front side of the magnetic attracting sheet. A sub-magic tape is provided on the front side of the upper platform. A mother magic tape is provided on the sub-magic tape, and an extension head is provided at the right end of the mother magic tape.

[0006] Preferably, an anti-slip pad is provided on the lower side of the base. Limiting frames are provided on the upper side of the base, and the limiting frames are arranged at equal intervals. A first connecting member is provided on the front upper side of the base, and the lower platform is rotatably connected to the inner front side of the first connecting member.

[0007] Preferably, a round hole is provided on the front side of the lower platform, and an insertion rod is provided on the round hole.

[0008] Preferably, a rotating structure is formed between the upper platform and the lower platform through fixing screws.

[0009] Preferably, there are three groups of the mother magic tape and the extension head.

[0010] Compared with the prior art, the beneficial effects of the present utility model are as follows: The platform used for non-invasive selective unilateral pulmonary drug administration in rats

[0011] (1) The present utility model can stably, locally, and repeatedly administer drugs to unilateral lung tissue. In this study, a unilateral drug administration model was made. The distribution of drugs in unilateral lung tissue was observed macroscopically by applying Evan’s blue dye, and a unilateral lung injury model was made using lipopolysaccharide. The expressions of caspase-1 and caspase-11 proteins were detected by immunohistochemistry in the left and right lungs. Combining with lipopolysaccharide can directly stimulate the increase of their expressions, and no increase in caspase-11 expression was found in the right lung. Multiple independent experiments prove the stability, locality, and repeatability of the present utility model;

[0012] (2) Unilateral lung injury is a real state in ARDS patients, accounting for about 25% of all ARDS patients, and it can progress to bilateral lung injury. The larger the injury range, the higher the fatality rate. Studying the injury mechanism of unilateral lung injury and inventing corresponding treatment measures have clinical transformation significance. Description of the Drawings

[0013] Figure 1This is the front view structural schematic diagram of the utility model;

[0014] Figure 2 This is the left view structural schematic diagram of the utility model;

[0015] Figure 3 This is the front view structural schematic diagram of the lower platform of the utility model;

[0016] Figure 4 This is the Figure 2 enlarged structural schematic diagram at position A in the utility model;

[0017] Figure 5 This is the Figure 2 enlarged structural schematic diagram at position B in the utility model.

[0018] In the figure: 1, base; 2, anti-slip pad; 3, limit frame; 4, first connecting piece; 5, lower platform; 6, second connecting piece; 7, support rod; 8, round hole; 9, insertion rod; 10, upper platform; 11, fixing screw; 12, magnetic attracting sheet; 13, limit rod; 14, rubber band; 15, male magic tape; 16, female magic tape; 17, extension head. Specific implementation manners

[0019] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments in the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0020] Please refer to Figures 1-5 , the present utility model provides a technical solution: a platform used when performing non-invasive selective unilateral pulmonary drug administration on rats. A second connecting piece 6 is provided on the rear side of the lower platform 5, a support rod 7 is rotatably connected to the second connecting piece 6, and the bottom end of the support rod 7 is inserted between the limit frames 3. An upper platform 10 is provided on the front side of the lower platform 5, and the upper platform 10 is connected to the lower platform 5 through fixing screws 11. A magnetic attracting sheet 12 is provided on the front side of the upper platform 10. A limit rod 13 is provided on the front side of the upper platform 10, and the limit rods 13 are respectively arranged on the left and right sides of the magnetic attracting sheet 12. A rubber band 14 is provided on the limit rod 13, and the rubber band 14 is arranged on the front side of the magnetic attracting sheet 12. A male magic tape 15 is provided on the front side of the upper platform 10, a female magic tape 16 is provided on the male magic tape 15, and an extension head 17 is provided at the right end of the female magic tape 16. The three groups of female magic tapes 16 can better fix and limit the body of the rat, so as not to affect the progress of the experiment.

[0021] Such as Figure 1 and Figure 2As shown in the figure, an anti-slip pad 2 is provided on the lower side of the base 1, and a limiting frame 3 is provided on the upper side of the base 1, and the limiting frames 3 are arranged at equal intervals. A first connecting member 4 is provided on the front upper side of the base 1, and a lower layer platform 5 is rotatably connected to the front inner side of the first connecting member 4, so that the overall stability of the base 1 is better.

[0022] As Figure 3 shown in the figure, a round hole 8 is provided on the front side of the lower layer platform 5, and a plug rod 9 is provided on the round hole 8. After the upper layer platform 10 is rotated to the specified position, by inserting the plug rod 9 into the round hole 8 inside the lower side of the upper layer platform 10, the upper layer platform 10 can be fixed.

[0023] As Figure 1 、 Figure 2 and Figure 3 shown in the figure, a rotating structure is formed between the upper layer platform 10 and the lower layer platform 5 through a fixing screw 11, so that the upper layer platform 10 can be conveniently rotated to adjust the position.

[0024] As Figure 1 shown in the figure, there are three groups of female magic tapes 16 and extension heads 17, so that the body of the rat can be better fixed and limited.

[0025] Working principle: When using the platform for non-invasive selective unilateral pulmonary drug delivery in rats, first anesthetize the rat. After the rat loses its anesthetic consciousness, put the rat between the male magic tapes 15, and use the rubber band 14 to limit the upper jaw of the rat to keep the rat's mouth open. Then use three groups of female magic tapes 16 to limit and fix the body of the rat. At this time, insert a special tracheal catheter with a total length of 12 cm and an outer diameter of 0.85 mm into the trachea of the rat through the oral cavity without trauma. The front end of the tracheal catheter is shaped into an angle of about 160 degrees, and the front end orientation is marked. Slowly penetrate it in the nine o'clock direction by adjusting the front end orientation, and fix the tracheal intubation at a position 8.5 cm from the rat's incisors at the front end. At this time, by rotating the upper layer platform 10, the left side of the rat is placed at a lower position, and then fix the upper layer platform 10 at a position 70 degrees from the midline of the intubation platform, and let the plug rod 9 insert into the corresponding round hole 8 inside to fix the upper layer platform 10. At this time, the experiment can be carried out. Multiple independent experiments have proved that for rats weighing about 300 g, the above-mentioned scheme can stably, locally and repeatedly deliver liquid to the unilateral lung.

[0026] Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A platform for non-invasive selective unilateral lung drug delivery in rats, comprising a base (1) and a lower platform (5), characterized in that: The rear side of the lower platform (5) is provided with a second connecting piece (6), the second connecting piece (6) is rotatably connected with a support rod (7), and the bottom end of the support rod (7) is inserted between the limit frames (3); the front side of the lower platform (5) is provided with an upper platform (10), and the upper platform (10) is connected to the lower platform (5) by a fixing screw (11); the front side of the upper platform (10) is provided with a magnetic attracting sheet (12), and the upper platform (10) A limiting rod (13) is arranged on the front side, and the limiting rod (13) is respectively arranged on the left and right sides of the magnetic attraction plate (12), a rubber band (14) is arranged on the limiting rod (13), and the rubber band (14) is arranged on the front side of the magnetic attraction plate (12), a sub-velcro (15) is arranged on the front side of the upper platform (10), a mother Velcro (16) is arranged on the sub-velcro (15), and an extension head (17) is arranged at the right end of the mother Velcro (16).

2. The platform for non-invasive selective unilateral lung drug delivery in rats according to claim 1, characterized in that: The lower side of the base (1) is provided with an anti-slip pad (2), the upper side of the base (1) is provided with a limit frame (3), and the limit frames (3) are arranged at equal intervals, and the front upper side of the base (1) is provided with a first connecting member (4), and the front inner side of the first connecting member (4) is rotatably connected to a lower platform (5).

3. The platform for non-invasive selective unilateral lung drug delivery in rats according to claim 1, characterized in that: A circular hole (8) is provided on the front side of the lower platform (5), and an insertion rod (9) is provided on the circular hole (8).

4. The platform for non-invasive selective unilateral lung drug delivery in rats according to claim 1, characterized in that: The upper platform (10) is a rotating structure with the lower platform (5) via fixing screws (11).

5. The platform for non-invasive selective unilateral lung drug delivery in rats according to claim 1, characterized in that: The female Velcro (16) and the extension head (17) are provided in three groups.

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