Anti-mouse complement C5 antibody detection kit
By designing an anti-mouse complement C5 antibody detection kit containing a removable box cover and box body, an opaque material box cover and enzyme label board, the complex operation and light exposure problems of detecting IP160C content in mice in the prior art is solved, and a simple and convenient ELISA detection and light protection environment are achieved.
Patent Information
- Application Number
- CN202421674945.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-15
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2034-07-15
AI Technical Summary
The prior art is difficult to provide a simple operation and light-proof ELISA detection kit to detect the content of IP160C in mice serum.
An anti-mouse complement C5 antibody detection kit is designed, which adopts a removable box cover and box body structure. The box cover is made of opaque material, including a pulling base and a drawer. The enzyme mark board is set in the draw groove. The box cover can be fitted outside the box body to provide a light-proof environment.
It realizes ELISA detection with simple and convenient operation, provides an appropriate light-proof environment, and ensures the safety of the enzyme label plate and the accuracy of the detection results.
Smart Images
Figure CN222965238U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of immunological detection kits, and particularly relates to a kit for detecting anti-mouse complement C5 antibody. Background Art
[0002] The complement system is a group of plasma and cell membrane proteins that play a key role in the immune system. All three pathways (classical, alternative, and lectin) lead to the cleavage of C3 and ultimately the formation of the cell-lytic membrane attack complex C5b-9. The C5 convertase cleaves C5, releasing the potent anaphylatoxin C5a and generating C5b. C5b can initiate the terminal pathway, which recruits components C6, C7, C8, and C9 to the target surface and inserts the C9 complex as a pore (termed the terminal complement complex) into the membrane. C5 is synthesized as a single polypeptide chain in the liver and is present in serum at a concentration of 50 - 80 μg / ml. The monoclonal antibody BB5.1 binds to C5 in normal mouse serum and inhibits C5-dependent hemolysis in functional complement assays.
[0003] IP160C is a modified version of the monoclonal antibody BB5.1 and is used as a substitute for the monoclonal antibody against mouse complement C5 in mouse pharmacokinetic experiments. To detect the metabolism of the drug for IP60C in mouse serum, the utility model designs an ELISA detection kit to detect the content of IP160C in mouse serum. Summary of the Utility Model
[0004] The problem to be solved by the utility model is to provide an ELISA detection kit that is simple and convenient to operate and light-shielding for detecting the content of IP160C in mouse serum.
[0005] To solve the above technical problems, the utility model provides the following technical solutions:
[0006] A kit for detecting anti-mouse complement C5 antibody, comprising a box cover and a box body. The box cover and the box body are detachably connected. The box cover is made of opaque material. The box cover includes a pulling base and a drawer. The pulling base and the drawer are connected by pulling. The pulling base includes a buckle cover at the bottom and a pulling hole provided on the buckle cover. The drawer can be pulled back and forth in the pulling hole. The buckle cover is buckled on the box body. An enzyme-labeled plate is detachably connected in the drawer. A detachable anti-vibration base is arranged in the box body. A plurality of placing holes are arranged in the anti-vibration base, and reagent bottles are placed in the placing holes. The reagent bottles contain reagents for ELISA detection added to the enzyme-labeled plate.
[0007] Further, the left side of the pulling hole is flush with the left side of the buckle cover, and the right side of the pulling hole is located inside the right side of the buckle cover; the drawer includes a pulling groove and a baffle. The baffle is connected to the right side of the pulling groove for closing the right side of the pulling groove. The thickness of the baffle is equal to the horizontal distance between the right side of the pulling hole and the right side of the buckle cover.
[0008] Furthermore, the ELISA plate is disposed in the extraction slot.
[0009] Furthermore, notch structures are provided on the front side and the rear side of the extraction slot, and the bottom of the notch structure is flush with the bottom of the extraction slot.
[0010] Furthermore, the notch structure is arc-shaped.
[0011] Furthermore, the anti-vibration base is made of foam material.
[0012] Furthermore, there are seven placement holes, including two placement holes No. 1, two placement holes No. 2, and three placement holes No. 3. The diameters decrease in sequence as placement hole No. 1, placement hole No. 2, and placement hole No. 3.
[0013] Furthermore, the box cover is a light-tight box cover made of light-impermeable material.
[0014] Furthermore, the box cover and the box body are in a fitting connection, and the box cover can be fitted on the outside of the box body to close the box body.
[0015] Furthermore, an integral fitting portion is provided at the top of the box body. The surface of the fitting portion is within the surface of the box body, and the box cover is fitted on the fitting portion.
[0016] Compared with the prior art, the present utility model has the following beneficial effects:
[0017] (1) The box cover of the present utility model is a detachable closed structure, and the box cover is made of opaque material, which can prevent light from entering the box cover, provide a light-shielding space for the ELISA plate, and provide a suitable light-shielding environment for the staining step in ELISA detection.
[0018] (2) The detachable design makes it convenient to take and place the ELISA plate during the experiment. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 It is a structural diagram of the anti-mouse complement C5 antibody detection kit of the present utility model;
[0020] Figure 2 It is a schematic diagram of the overall structure of the box cover of the present utility model;
[0021] Figure 3 It is a schematic diagram of the pulling base structure of the present utility model;
[0022] Figure 4 It is a schematic diagram of the drawer structure of the present utility model;
[0023] Figure 5 It is a schematic diagram of the box body structure of the present utility model;
[0024] Figure 6 It is a schematic diagram of the anti-vibration base structure of the present utility model.
[0025] Reference numerals:
[0026] 1 - Lid of the box; 11 - Drawer base; 111 - Buckle cover; 112 - Drawer hole; 12 - Drawer; 121 - Drawer slot; 122 - Baffle; 123 - Notch structure;
[0027] 2 - Box body; 21 - Fitting part; 3 - Enzyme - labeled plate; 4 - Anti - shock base; 41 - First placement hole; 42 - Second placement hole; 43 - Third placement hole. Detailed implementation manners
[0028] Next, the technical solutions of the present utility model will be clearly described in conjunction with the accompanying drawings. Obviously, the described embodiments are not all embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present utility model.
[0029] It should be noted that the orientation or positional relationships indicated by the terms "center", "upper", "lower", "horizontal", "left", "right", "front", "rear", "lateral", "longitudinal", etc. are based on the orientation or positional relationships shown in the accompanying drawings. These are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of the present utility model.
[0030] This embodiment provides a kit for detecting anti - murine complement C5 antibody. The present utility model will be further described in conjunction with the accompanying drawings.
[0031] As Figure 1 shown, the ELISA kit includes a lid 1 of the box and a box body 2. The lid 1 of the box and the box body 2 are detachably connected, which is convenient for the connection and disassembly of the lid 1 of the box and the box body 2. More specifically, the lid 1 of the box and the box body 2 are fitted and connected, and the lid 1 of the box can be fitted outside the box body 2 to close the box body 2.
[0032] Combined with Figures 2 - 4 shown, the lid 1 of the box is of a draw - out type and includes a drawer base 11 and a drawer 12. The drawer base 11 and the drawer 12 are draw - connected, and the drawer 12 can be drawn back and forth in the drawer base 11.
[0033] As Figure 3 shown, specifically, the drawer base 11 includes a buckle cover 111 at the bottom and a drawer hole 112 provided on the buckle cover 111. The right side of the drawer hole 112 is in an open - mouth shape, and the rest of the drawer hole 112 is in a closed structure.
[0034] As Figure 3As shown, the left side of the pulling hole 112 is flush with the left side of the cover 111, and the right side of the pulling hole 112 is located inside the right side of the cover 111.
[0035] As Figure 3 shown, the drawer 12 includes a drawer slot 121 and a baffle 122. The baffle 122 is connected to the right side of the drawer slot 121 and is used to close the right side of the drawer slot 121.
[0036] Combined with Figures 2 - 4 shown, the thickness of the baffle 122 is equal to the horizontal distance between the right side of the pulling hole 112 and the right side of the cover 111. When the drawer 12 is inserted into the pulling base 11, the sizes of the drawer slot 121 and the pulling hole 112 match, and the drawer slot 121 is located inside the pulling hole 112; the bottom of the baffle 122 just covers the area between the right side of the pulling hole 112 and the right side of the cover 111.
[0037] Combined with Figures 2 - 4 shown, a detachable enzyme plate 3, which is a 96-well plate, is provided inside the box cover 1; the enzyme plate 3 is arranged in the drawer slot 121. The extractable design makes it convenient to take and place the enzyme plate 3 during the experiment.
[0038] The box cover 1 is made of an opaque material, using a light-blocking material, and the box cover 1 can be completely closed, providing a light-shielded space for the enzyme plate 3. The box cover 1 can provide a suitable light-shielded environment for the staining step in ELISA detection, which is convenient and fast to use.
[0039] Notch structures are provided on both the front side and the rear side of the drawer slot 121. The notch structures are arc-shaped, and the bottom of the notch structures is flush with the bottom of the drawer slot 121, thus facilitating the taking and placing of the enzyme plate 3 in the drawer slot 121.
[0040] As Figure 5 shown, the box body 2 is of an inlaid type. The top of the box body 2 is provided with an integral inlaid part 21, and the surface of the inlaid part 21 is located inside the surface of the box body 2, facilitating the box cover 1 to be inlaid on the inlaid part 21.
[0041] A detachable anti-vibration base 4 is provided inside the cavity of the box body 2.
[0042] As Figure 6 shown, seven placement holes are provided inside the anti-vibration base 4, and each placement hole is used to internally place a reagent bottle; the anti-vibration base 4 is made of foam material and is used to prevent the reagent in the reagent bottle from vibrating.
[0043] The upper row of the seven placement holes, from left to right, are: two placement holes No. 1 41 and two placement holes No. 2 42; the lower row is three placement holes No. 3 43, and the diameters decrease in order from the placement hole No. 1 41, the placement hole No. 2 42, to the placement hole No. 3 43.
[0044] The seven reagent bottles for containing reagents are respectively two standard quality control tubes, an antibody tube, a sample diluent bottle, a cleaning solution bottle, a chromogenic substrate bottle, and a stop solution bottle.
[0045] The seven reagent bottles include two large bottles, two medium bottles, and three small bottles. The seven reagent bottles are correspondingly placed into the placement holes. The two large bottles respectively contain sample diluent and cleaning solution, and are placed in the two placement holes No. 41 from left to right in sequence; the two medium bottles respectively contain developing solution and stop solution, and are placed in the two placement holes No. 42 from left to right in sequence; the three small bottles respectively contain antibody and two standards, and are placed in the two placement holes No. 43 from left to right in sequence.
[0046] The ELISA test kit is in a cuboid structure, and can also be designed into other shapes according to the needs of ELISA detection.
[0047] The method for performing ELISA detection using the ELISA test kit of the present utility model may include the following steps:
[0048] During the process of performing ELISA detection,
[0049] First, dilute the standard with the diluent in the diluent bottle to obtain standard solutions with different concentrations.
[0050] Similarly, dilute the sample to be tested to the corresponding multiple with the diluent, and then add it to the enzyme-linked immunosorbent assay (ELISA) plate 3.
[0051] Place the ELISA plate 3 into the extractable lid 1 for incubation.
[0052] After that, perform a conventional ELISA detection.
[0053] Dilute the cleaning solution with purified water to the corresponding multiple, and wash the ELISA plate 3 three times with the diluted cleaning solution.
[0054] After that, operate according to the conventional ELISA detection.
[0055] Add the antibody and wash. Add the freshly prepared chromogenic substrate to the ELISA plate 3, place it in the extractable lid 1, and perform the chromogenic reaction in the dark.
[0056] After that, operate according to the conventional ELISA detection.
[0057] Add the stop solution. Add the stop solution to the ELISA plate 3 to terminate the chromogenic reaction.
[0058] After that, operate according to the conventional ELISA detection.
[0059] The above technical features constitute the best embodiment of the present utility model, which has strong adaptability and the best implementation effect. Non-essential technical features can be increased or decreased according to actual needs to meet the requirements of different situations.
[0060] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present utility model, rather than limiting the protection scope of the present utility model. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present utility model shall not depart from the essence and scope of the technical solution of the present utility model.
Claims
1. An anti-mouse complement C5 antibody detection kit, comprising a box cover and a box body, wherein the box cover and the box body are detachably connected, characterized in that: The box cover is made of opaque material and includes a pull-out base and a drawer. The pull-out base and the drawer are connected by pull-out connection. The pull-out base includes a buckle cover at the bottom and a pull-out hole set on the buckle cover. The drawer can be pulled back and forth in the pull-out hole. The buckle cover is buckled on the box body. An ELISA plate is detachably connected to the drawer. A detachable anti-vibration base is provided in the box body. Several placement holes are provided in the anti-vibration base. The placement holes are used to place reagent bottles. The reagent bottles contain reagents added to the ELISA plate for ELISA testing.
2. The anti-mouse complement C5 antibody detection kit according to claim 1, characterized in that: The left side of the pull-out hole is flush with the left side of the buckle cover, and the right side of the pull-out hole is located inside the right side of the buckle cover; the drawer includes a drawer slot and a baffle, the baffle is connected to the right side of the drawer slot and is used to close the right side of the drawer slot, and the thickness of the baffle is equal to the horizontal distance between the right side of the pull-out hole and the right side of the buckle cover.
3. The anti-mouse complement C5 antibody detection kit according to claim 2, characterized in that: The ELISA plate is arranged in the drawer.
4. The anti-mouse complement C5 antibody detection kit according to claim 2, characterized in that: Notch structures are arranged on the front side and the rear side of the draw groove, and the bottom of the notch structure is flush with the bottom of the draw groove.
5. The anti-mouse complement C5 antibody detection kit according to claim 4, characterized in that: The notch structure is arc-shaped.
6. The anti-mouse complement C5 antibody detection kit according to claim 1, characterized in that: The anti-shock base is made of foam.
7. The anti-mouse complement C5 antibody detection kit according to claim 1, characterized in that: There are seven placement holes, including two placement holes No. 1, two placement holes No. 2, and three placement holes No.
3. The diameters from large to small are placement hole No. 1, placement hole No. 2, and placement hole No.
3.
8. The anti-mouse complement C5 antibody detection kit according to claim 1, characterized in that: The box cover is a light-tight box cover made of opaque material.
9. The anti-mouse complement C5 antibody detection kit according to claim 1, characterized in that: The box cover and the box body are in a chimeric connection, and the box cover can be chimerically engaged with the outside of the box body to close the box body.
10. The anti-mouse complement C5 antibody detection kit according to claim 9, characterized in that: An integral fitting portion is arranged on the top of the box body, the surface of the fitting portion is located inside the surface of the box body, and the box cover is embedded on the fitting portion.