Continuous flow ultrasonication device
By designing a continuous flow ultrasonic crushing device, the pollution and virus stability problems of traditional ultrasonic devices when crushing the varicella-zoster virus are solved, and an efficient virus crushing process without pollution and infection is achieved.
Patent Information
- Application Number
- CN202422030606.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Priority Date
- 2024-07-17
- Filing Date
- 2024-08-20
- Publication Date
- 2025-06-20
- Estimated Expiration
- 2034-08-20
AI Technical Summary
When traditional ultrasound devices break up viruses such as varicella-zoster virus, there is a risk of sample contamination and virus leakage, and the virus is unstable and is prone to inactivate after repeated freeze-thawing.
A continuous flow ultrasonic crushing device is designed, including a reaction box, ultrasonic cell crusher host, transducer, amplitude rod, liquid inlet bottle, liquid outlet bottle and power source, and ultrasonic processing is carried out through a sealed environment to avoid virus leakage.
The virus fragmentation process without contamination and infection is achieved, the activity and stability of the virus is maintained, and it is suitable for large-scale cell fragmentation production.
Smart Images
Figure CN223002932U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the technical field of biomedical equipment, and particularly to a continuous-flow ultrasonic cell disruption device. Background Art
[0002] In the biomedical field, especially during the production of vaccines, the harvest of intracellular culture products requires the disruption of host cell bodies to release the culture products. Common cell disruption methods include freeze-thaw method, enzymatic lysis, chemical permeation, and ultrasonic disruption, etc.
[0003] As physical methods that do not require the addition of exogenous substances, freeze-thaw and ultrasound are often the preferred methods. However, due to the special properties of certain viruses, such as varicella-zoster virus, which is prone to bind to cells, it is difficult to obtain free live viruses. At the same time, the virus is relatively unstable and is prone to inactivation after repeated freeze-thaw cycles.
[0004] Traditional ultrasonic devices only place the sample in an ice bath and insert the ultrasonic probe into the sample to disrupt cells in an open and small-scale manner, posing a risk of sample contamination and virus leakage. Utility Model Content
[0005] In order to provide a sealed and pollution-free environment for production, this application provides a continuous-flow ultrasonic cell disruption device.
[0006] The continuous-flow ultrasonic cell disruption device provided by this application adopts the following technical solutions:
[0007] A continuous-flow ultrasonic cell disruption device includes a reaction tank, a main body of an ultrasonic cell disruptor, a transducer, a horn, a liquid inlet bottle, a liquid outlet bottle, and a power source for transmitting the solution. The reaction tank is provided with a liquid inlet and a liquid outlet. The liquid inlet is connected to the liquid inlet bottle, the liquid outlet is connected to the liquid outlet bottle. The main body of the ultrasonic cell disruptor is connected to the transducer, one end of the horn is connected to the transducer, and the other end of the horn extends into the cell solution.
[0008] By adopting the above technical solutions, the cell solution enters the reaction tank from the liquid inlet bottle. The horn performs ultrasonic treatment on the cell solution. The treated cell solution enters the liquid outlet bottle. Since the treatment process is in a sealed environment, it avoids the problem of exposure during the treatment process and causing infection.
[0009] Optionally, a cooling tank is provided outside the reaction tank. The cooling tank is provided with a cooling water inlet and a cooling water outlet. A cooling and heat preservation tank for the flow of cooling water is provided between the outer wall of the reaction tank and the inner wall of the cooling tank.
[0010] By adopting the above technical solutions, the cooling water introduced into the cooling and heat preservation layer can continuously cool the reaction tank and maintain its appropriate temperature.
[0011] Optionally, a connection groove is provided on the transducer, and the horn is detachably installed in the connection groove.
[0012] By adopting the above technical solution, the horn can be disassembled or sterilized in place.
[0013] Optionally, the length of the horn extending below the liquid level of the cell solution ranges from 5 cm to 6 cm.
[0014] By adopting the above technical solution, a larger ultrasonic treatment range is ensured.
[0015] Optionally, the power source is a peristaltic pump.
[0016] By adopting the above technical solution, the peristaltic pump is convenient to operate and is also convenient to control the infusion speed.
[0017] Optionally, a temperature sensor is provided in the cooling and heat preservation tank.
[0018] By adopting the above technical solution, it is convenient for the staff to monitor the temperature of the cooling water.
[0019] Optionally, the liquid outlet of the liquid outlet bottle is connected to the liquid inlet of the liquid inlet bottle.
[0020] By adopting the above technical solution, the liquid inlet bottle and the liquid outlet bottle are connected to each other to realize multiple cycles of ultrasonic treatment.
[0021] Optionally, a partition plate is provided between the cooling water inlet and the cooling water outlet. The partition plate is fixedly connected in the cooling and heat preservation tank and divides the cooling and heat preservation tank into two parts with the same area. A communication hole is provided on the partition plate, and the communication hole is located at one end of the partition plate close to the bottom of the cooling and heat preservation tank.
[0022] By adopting the above technical solution, the cooling and heat preservation tank can be divided into two parts with the same area by the partition plate. Furthermore, the partition plate can prevent the cold water entering from the cooling water inlet from directly discharging from the cooling water outlet. The cold water can be moved to the bottom of the cooling and heat preservation tank and pass through the communication hole, thereby improving the utilization efficiency of the cold water.
[0023] Optionally, a filter screen is provided at the communication hole, and a fixing component for fixing the filter screen on the through hole is provided at the communication hole. A support frame for supporting the cooling box is provided at the bottom of the cooling box. The bottom of the cooling box is threadedly connected and communicated with a maintenance pipe. A valve is installed on the maintenance pipe, and the maintenance pipe is located directly below the through hole.
[0024] By adopting the above technical solution, the water passing through the connecting hole can be filtered through the filter net, and the impurities at the bottom of the cooling box can be regularly cleaned by controlling the valve. At the same time, the maintenance rod can be removed from the bottom of the cooling box by rotating the maintenance pipe, and then the filter net can be removed by adjusting the fixing component here, and then the filter net can be replaced.
[0025] Optionally, a fixed ring groove is provided on the partition plate, and the fixed ring groove is arranged around the connecting hole. The fixed component includes two rotating plates, and the rotating plates are rotatably connected to the partition plate. The rotating plates are respectively located on both sides of the filter net, and the rotating plates press the filter net tightly into the fixed ring groove.
[0026] By adopting the above technical solution, the filter screen can be confined in the fixed ring groove by the rotating plate. At the same time, when removing the filter screen, the rotating rod can be moved by using an external rod to rotate the rotating rod and disengage the rotating rod from the abutment with the filter screen, thereby removing the filter screen from the connecting hole.
[0027] In summary, the present application includes at least one of the following beneficial technical effects:
[0028] 1. The cell solution enters the reaction box from the liquid inlet bottle, and the horn performs ultrasonic treatment on the cell solution. The treated cell solution enters the liquid outlet bottle. Since the treatment process is in a sealed environment, the problem of exposure during the treatment process and infection is avoided;
[0029] 2. The cooling water introduced into the cooling and insulation layer can continuously cool down the reaction box and maintain its suitable temperature;
[0030] 3. The liquid inlet bottle and the liquid outlet bottle are connected to each other to achieve multiple cycles of ultrasonic treatment. BRIEF DESCRIPTION OF THE DRAWINGS
[0031] Figure 1 It is a schematic structural diagram of a continuous flow ultrasonic fragmentation device according to Example 1 of the present application.
[0032] Figure 2 It is a schematic diagram of the overall structure of Example 2 of the present application;
[0033] Figure 3 is a schematic structural diagram of a partition board according to Example 2 of the present application;
[0034] Figure 4 It is a schematic diagram of the structure of the fixing component of Example 2 of the present application.
[0035] Description of reference numerals: 1, reaction chamber; 10, liquid inlet; 11, liquid outlet; 2, main body of ultrasonic cell disruptor; 3, transducer; 30, connecting groove; 4, horn; 5, cooling tank; 50, cooling water inlet; 51, cooling water outlet; 52, cooling and heat preservation tank; 53, maintenance pipe; 531, valve; 6, liquid inlet bottle; 7, liquid outlet bottle; 8, partition board; 81, communication hole; 82, filter screen; 83, fixed ring groove; 84, fixing component; 841, rotating plate; 9, support frame. Detailed implementation manners
[0036] The following further elaborates on this application Figure 1 - with reference to Figure 4 for a more detailed description.
[0037] The embodiment of this application discloses a continuous-flow ultrasonic disruption device.
[0038] Embodiment 1
[0039] Referring to Figure 1 , a continuous-flow ultrasonic disruption device includes a reaction chamber 1, a main body of an ultrasonic cell disruptor 2, a transducer 3, a horn 4, a cooling tank 5, a liquid inlet bottle 6, and a liquid outlet bottle 7.
[0040] Referring to Figure 1 , a liquid inlet 10 and a liquid outlet 11 are provided at the top of the reaction chamber 1. The liquid inlet bottle 6 is connected to the liquid inlet 10 through a sterile pipeline and a quick connector, and the liquid outlet bottle 7 is connected to the liquid outlet 11 through a sterile pipeline and a quick connector. A peristaltic pump or other pressure structure can provide power and adjust the liquid inlet speed. When needed, the liquid inlet bottle 6 and the liquid outlet bottle 7 can be connected to each other to achieve multiple cycles of ultrasonic treatment.
[0041] Referring to Figure 1 , the transducer 3 is arranged at the middle position at the top of the reaction chamber 1. The transducer 3 is connected to the main body of the ultrasonic cell disruptor 2. A connecting groove 30 is provided at the bottom of the transducer 3, and the bottom of the connecting groove 30 extends into the interior of the reaction chamber 1. The horn 4 is detachably installed in the connecting groove 30, achieving airtight closure, and can be disassembled or sterilized in place. The horn 4 vertically extends 5 cm to 6 cm below the liquid level of the cell suspension.
[0042] Referring to Figure 1 , a cooling tank 5 is arranged outside the reaction chamber 1. A cooling water inlet 50 and a cooling water outlet 51 are provided at the top of the cooling tank 5. A cooling and heat preservation tank 52 is arranged between the inner wall of the cooling tank 5 and the outer wall of the reaction chamber 1. The staff can connect low-temperature cooling water through the cooling water inlet 50, and cool down and sound-insulate the reaction chamber 1 through the flowing cooling water. A temperature sensor can also be installed in the cooling and heat preservation tank 52 to detect the water temperature in real time.
[0043] The implementation principle of Embodiment 1 is as follows: When the staff performs cell disruption, first connect the liquid inlet bottle 6 to the liquid inlet 10 of the reaction tank 1, and the liquid outlet bottle 7 to the liquid outlet 11 of the reaction tank 1. Then connect the cooling water to the cooling water inlet 50 of the cooling tank 5, and the cooling water flows into the cooling and heat preservation tank 52, thereby cooling the reaction tank 1. After all the pipelines are connected, the liquid in the liquid inlet bottle 6 is introduced into the reaction tank 1 through a power source such as a peristaltic pump. At the same time, turn on the main unit 2 of the ultrasonic cell disruptor, and the horn 4 performs ultrasonic treatment on the solution. The treated solution enters the liquid outlet bottle 7 through the liquid outlet 11.
[0044] The output power of the main unit 2 of the ultrasonic cell disruptor, the model of the horn 4, and the number of times of circulating treatment of the cell solution can be adjusted as needed. This disruption device is easy to use, ensures a closed and sterile environment during the ultrasonic process, and has the characteristics of sustainable cooling, low noise, and can be used for large-scale cell disruption production.
[0045] Embodiment 2
[0046] The difference from the embodiment is that: Referring to Figure 2 and Figure 3 , a partition plate 8 is provided between the cooling water inlet 50 and the cooling water outlet 51, and the partition plate 8 is arranged in a concave shape. The partition plate 8 is fixedly connected to the inner side wall of the cooling and heat preservation tank 52. The partition plate 8 divides the cooling and heat preservation tank 52 into two parts with the same area. Two communication holes 81 are opened on the partition plate 8. The two communication holes 81 are respectively located on both sides of the partition plate 8, and the communication holes 81 are located at one end of the partition plate 8 close to the bottom of the cooling and heat preservation tank 52.
[0047] Referring to Figure 2 , Figure 3 and Figure 4 , two fixing ring grooves 83 are opened on the partition plate 8, and the fixing ring grooves 83 correspond to the communication holes 81 one by one. The fixing ring grooves 83 are arranged around the edge of the communication holes 81. A filter screen 82 is provided in each fixing ring groove 83, and a fixing component 84 for fixing the filter screen 82 on the through hole is provided at the communication hole 81.
[0048] The fixing component 84 includes two rotating plates 841. The rotating plates 841 are respectively located on both sides of the filter screen 82 and the rotating plates 841 are rotatably connected to the partition plate 8. The rotating plates 841 press the filter screen 82 tightly in the fixing ring groove 83.
[0049] A support frame 9 for supporting the cooling tank 5 is provided at the bottom of the cooling tank 5. A maintenance pipe 53 is threadedly sealed at the bottom of the cooling tank 5, and the maintenance pipe 53 is communicated with the inside of the cooling tank 5. A valve 531 is installed on the maintenance pipe 53. The maintenance pipe 53 is located directly below the communication hole 81 and the maintenance pipe 53 corresponds to the communication hole 81 one by one.
[0050] The implementation principle of Example 2 is as follows: the cold water added into the cooling and heat preservation tank 52 from the cooling water inlet 50 can move to the side of the bottom of the cooling and heat preservation tank 52, and move to the side of the cooling water outlet 51 through the filtration of the filter screen 82 at the connecting hole 81. At the same time, when the filter screen 82 is replaced, the maintenance tube 53 can be rotated to remove the maintenance tube 53 from the cooling box 5. Then, through the hole generated by the disassembly and using an external rod-like workpiece to move the rotating plate 841, the rotating plate 841 is no longer in contact with the filter screen 82, and then the filter screen 82 is taken out from the cooling box 5, thereby completing the removal of the filter screen 82.
[0051] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.
Claims
1. A continuous flow ultrasonic crushing device, characterized in that: The invention comprises a reaction box (1), an ultrasonic cell disruptor main unit (2), a transducer (3), an amplitude rod (4), a liquid inlet bottle (6), a liquid outlet bottle (7) and a power source for transmitting a solution. The reaction box (1) is provided with a liquid inlet (10) and a liquid outlet (11), the liquid inlet (10) is connected to the liquid inlet bottle (6), the liquid outlet (11) is connected to the liquid outlet bottle (7), the ultrasonic cell disruptor main unit (2) is connected to the transducer (3), one end of the amplitude rod (4) is connected to the transducer (3), and the other end of the amplitude rod (4) extends into the cell solution.
2. The continuous flow ultrasonic crushing device according to claim 1, characterized in that: A cooling box (5) is arranged outside the reaction box (1), and a cooling water inlet (50) and a cooling water outlet (51) are arranged on the cooling box (5). A cooling and heat preservation groove (52) for cooling water to flow is arranged between the outer wall of the reaction box (1) and the inner wall of the cooling box (5).
3. The continuous flow ultrasonic crushing device according to claim 1, characterized in that: The transducer (3) is provided with a connecting groove (30), and the amplitude transformer (4) is detachably mounted in the connecting groove (30).
4. The continuous flow ultrasonic crushing device according to claim 1, characterized in that: The length of the horn (4) extending below the cell solution surface ranges from 5 cm to 6 cm.
5. The continuous flow ultrasonic crushing device according to claim 1, characterized in that: The power source is a peristaltic pump.
6. The continuous flow ultrasonic crushing device according to claim 2, characterized in that: A temperature sensor is arranged in the cooling and heat preservation tank (52).
7. The continuous flow ultrasonic crushing device according to claim 1, characterized in that: The liquid outlet (11) of the liquid outlet bottle (7) and the liquid inlet (10) of the liquid inlet bottle (6) are connected to each other.
8. The continuous flow ultrasonic crushing device according to claim 2, characterized in that: A partition plate (8) is provided between the cooling water inlet (50) and the cooling water outlet (51); the partition plate (8) is fixedly connected to the cooling and heat-insulating groove (52) and the partition plate (8) divides the cooling and heat-insulating groove (52) into two parts of equal area; a connecting hole (81) is provided on the partition plate (8); the connecting hole (81) is located at one end of the partition plate (8) close to the bottom of the cooling and heat-insulating groove (52).
9. The continuous flow ultrasonic crushing device according to claim 8, characterized in that: A filter screen (82) is provided at the communicating hole (81), and a fixing assembly (84) for fixing the filter screen (82) on the communicating hole (81) is provided at the communicating hole (81); a support frame (9) for supporting the cooling box (5) is provided at the bottom of the cooling box (5); a maintenance pipe (53) is threadedly connected and connected to the bottom of the cooling box (5); a valve (531) is installed on the maintenance pipe (53); and the maintenance pipe (53) is located directly below the communicating hole (81).
10. The continuous flow ultrasonic crushing device according to claim 9, characterized in that: The partition plate (8) is provided with a fixed annular groove (83), the fixed annular groove (83) being arranged around the connecting hole (81), the fixed assembly (84) comprising two rotating plates (841), the rotating plates (841) being rotatably connected to the partition plate (8), the rotating plates (841) being respectively located on both sides of the filter screen (82), and the rotating plates (841) press the filter screen (82) tightly against the fixed annular groove (83).