Auxiliary bracket for changing liquid of cell culture plate

Abstract

The utility model relates to an auxiliary support, in particular to an auxiliary support for liquid change of a cell culture plate. The utility model aims to solve the problems that an operator is easy to fatigue and the working efficiency is low in the liquid changing process of the existing cell culture plate, so that the working difficulty of liquid changing is high. The liquid changing device is simple in structure, the cam is driven to rotate when the hand wheel is screwed, then the angle between the supporting plate and the rack is adjusted, the problem that an operator is prone to fatigue when holding a culture plate for a long time is solved, and then the liquid changing work efficiency is improved. When the pipette is used for adding liquid to the cell culture plate, the outer circumferential surface of the pipette head can be propped against the groove on the cross beam of the portal frame, so that the hand of an operator is more stable, the pipette head is prevented from touching the hole wall of the culture plate, and the culture liquid in the pipette is prevented from being polluted. The utility model belongs to the technical field of cell culture auxiliary devices.

Description

Technical Field

[0001] The utility model relates to an auxiliary bracket, in particular to an auxiliary bracket for liquid change of a cell culture plate, belonging to the technical field of cell culture auxiliary devices. Background Art

[0002] During the cell culture process, as time goes by, the nutrients in the culture medium will be consumed by the cells, and at the same time, the waste products generated by cell metabolism will also accumulate in the culture medium. If these waste products are not removed in time, it will have an adverse impact on the growth and health of the cells. Therefore, the culture medium in the cell culture plate needs to be replaced regularly.

[0003] When changing the culture medium, attention should be paid to aseptic operation. First, the operator tilts the cell culture plate, uses the tip of the pipette gun to press against the hole wall, and aspirates the waste liquid. Then, the tip is replaced, and the new culture medium is added to the hole. To avoid contamination of the culture medium in the tip by the substances in the hole, the culture medium needs to be added to the hole at a distance during liquid addition, and the distance between the tip and the hole wall during liquid addition cannot be too large, otherwise air bubbles are likely to appear when the culture medium is added to the hole, which is not conducive to the growth of the cells.

[0004] However, during the liquid change process, due to the large number of holes in some models of cell culture plates (such as 48-well plates and 96-well plates), it is easy for the operator to get tired when holding the culture plate for a long time, resulting in low liquid change work efficiency; secondly, during the long-term liquid change operation, the hand holding the pipette gun is likely to shake, making it inconvenient to control the distance between the tip and the hole wall, further increasing the difficulty of the liquid change work.

[0005] In summary, how to propose a pressurizing device for the above technical problems has become an urgent problem to be solved by those skilled in the art at present. Content of the Utility Model

[0006] The utility model aims at the above deficiencies of the prior art and provides an auxiliary bracket for liquid change of a cell culture plate.

[0007] The technical solution of the utility model is: an auxiliary bracket for liquid change of a cell culture plate, including a frame, the frame includes a first cross beam, a second cross beam, a first longitudinal beam, a second longitudinal beam and four support legs, the first cross beam, the second cross beam, the first longitudinal beam and the second longitudinal beam are fixedly connected into a rectangular frame, and the support legs are fixedly connected with the rectangular frame.

[0008] Furthermore, it further includes a tray, a gantry and an angle adjustment unit.

[0009] The tray is hinged to the first cross beam, the tray is used for storing the cell culture plate, and a strip-shaped limiting block and two slide rails are integrally arranged on the upper surface of the tray.

[0010] The vertical column of the gantry is slidably connected to the slide rail, and the vertical column of the gantry is perpendicularly arranged with the pallet. A plurality of grooves for the tip of the pipette to abut against are formed on the cross beam of the gantry.

[0011] The angle adjustment unit includes a mandrel, a handwheel, a rubber washer, a flat washer, a nut and a cam.

[0012] The mandrel is rotatably connected to the rectangular frame. The handwheel, the rubber washer, the flat washer, the nut and the cam are coaxially installed on the mandrel in sequence. The handwheel and the rubber washer are respectively arranged on two side surfaces of the first longitudinal beam. The outer circumferential surface of the cam abuts against the lower surface of the pallet.

[0013] Furthermore, the cross section of the slide rail is T-shaped. A T-shaped groove is formed on the vertical column of the gantry. The T-shaped groove is slidably connected to the slide rail.

[0014] The utility model has the following effects compared with the prior art:

[0015] 1. The structure of the utility model is simple. When the handwheel 310 is screwed, the cam 350 is driven to rotate, so as to adjust the angle between the pallet 200 and the frame 100, avoid the problem that the operator is easy to get tired when holding the culture plate for a long time, and improve the efficiency of the liquid changing work.

[0016] 2. The utility model is convenient for liquid changing operation. When adding liquid to the cell culture plate with a pipette, the outer circumferential surface of the pipette tip can be abutted against the groove on the cross beam of the gantry 230, making the operator's hand more stable, avoiding the pipette tip from touching the hole wall of the culture plate, and further avoiding polluting the culture solution in the pipette. Description of the Drawings

[0017] Figure 1 is the first axonometric drawing of the utility model;

[0018] Figure 2 is Figure 1 the partial enlarged drawing at I in

[0019] Figure 3 is the second axonometric drawing of the utility model;

[0020] Figure 4 is the schematic diagram of the arrangement of the handwheel 310, the rubber washer 320, the flat washer 330 and the nut 340 on the mandrel 300 of the utility model.

[0021] In the figure: 100, frame; 110, first cross beam; 120, second cross beam; 130, first longitudinal beam; 140, second longitudinal beam; 200, pallet; 210, strip-shaped limiting block; 220, slide rail; 230, gantry; 300, mandrel; 310, handwheel; 320, rubber washer; 330, flat washer; 340, nut; 350, cam. Detailed Embodiment

[0022] In order to make the utility model purpose, features, and advantages of the present utility model more obvious and understandable, the technical solutions in the embodiments of the present utility model will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present utility model.

[0023] Specific Embodiment 1: In combination with Figures 1 to 4 To illustrate this embodiment, an auxiliary support for liquid change of a cell culture plate in this embodiment includes a frame 100. The frame 100 includes a first cross beam 110, a second cross beam 120, a first longitudinal beam 130, a second longitudinal beam 140, and four support legs. The first cross beam 110, the second cross beam 120, the first longitudinal beam 130, and the second longitudinal beam 140 are fixedly connected to form a rectangular frame, and the support legs are fixedly connected to the rectangular frame.

[0024] Furthermore, it further includes a tray 200, a gantry 230, and an angle adjustment unit.

[0025] The tray 200 is hinged to the first cross beam 110. The tray 200 is used for storing cell culture plates. A strip-shaped limiting block 210 and two slide rails 220 are integrally provided on the upper surface of the tray 200. The length direction of the strip-shaped limiting block 210 is parallel to the rotation axis of the tray 200. With such a setting, when the cell culture plate is placed on the tray 200, the side surface of the cell culture plate abuts against the strip-shaped limiting block 210, avoiding the cell culture plate slipping on the tray 200 when an angle is generated between the tray 200 and the frame 100.

[0026] The columns of the gantry 230 are slidably connected to the slide rails 220, and the columns of the gantry 230 are perpendicularly arranged to the tray 200. A plurality of grooves for the tips of pipette tips to abut against are formed on the cross beam of the gantry 230.

[0027] The angle adjustment unit includes a core shaft 300, a handwheel 310, a rubber washer 320, a flat washer 330, a nut 340, and a cam 350.

[0028] The core shaft 300 is rotatably connected to the rectangular frame. The handwheel 310, the rubber washer 320, the flat washer 330, the nut 340, and the cam 350 are coaxially installed on the core shaft 300 in sequence, and the handwheel 310 and the rubber washer 320 are respectively arranged on both side surfaces of the first longitudinal beam 130. With such a setting, when the nut 340 is tightened, the nut 340 and the flat washer 330 exert a squeezing force on the rubber washer 320, causing the rubber washer 320 to deform, thereby increasing the friction force between the rubber washer 320 and the first cross beam 110, and avoiding the cam 350 deflecting due to the gravity of the tray 200 and the cell culture plate when the handwheel 310 is loosened.

[0029] The outer circumferential surface of the cam 350 abuts against the lower surface of the support plate 200. With such a setting, turning the handwheel 310 can drive the cam 350 to rotate, thereby adjusting the angle between the support plate 200 and the frame 100.

[0030] In this embodiment, the cross-section of the crossbeam of the gantry 230 is rectangular, and a number of grooves for the tip of the pipette to abut against are provided on both side vertical surfaces of the crossbeam, and the grooves are equally spaced along the length direction of the crossbeam. With such a setting, when adding liquid to the cell culture plate with a pipette, to prevent the tip of the pipette from touching the pore wall of the cell culture plate due to the shaking of the operator's hand, the outer circumferential surface of the tip of the pipette can be abutted against the groove, making the operator's hand more stable, thereby avoiding contaminating the culture solution in the pipette.

[0031] Specific Embodiment Two: Combining Figures 1 to 3 To illustrate this embodiment, in this embodiment, the cross-section of the slide rail 220 is T-shaped, and a T-shaped groove is provided on the column of the gantry 230, and the T-shaped groove is slidably connected to the slide rail 220. The other components and connection relationships are the same as those in Specific Embodiment One.

[0032] Specific Embodiment Three: Combining Figure 1 and Figure 2 To illustrate this embodiment, in this embodiment, reinforcing ribs are provided on the crossbeam of the gantry 230. With such a setting, the stiffness of the crossbeam is increased, and deformation of the crossbeam during use is avoided. The other components and connection relationships are the same as those in Specific Embodiment One or Two.

[0033] Specific Embodiment Four: Combining Figures 1 to 4 To illustrate this embodiment, in this embodiment, the materials of the frame 100, the support plate 200, the gantry 230, and the cam 350 are all polystyrene.

[0034] Furthermore, the material of the mandrel 300 is stainless steel.

[0035] The other components and connection relationships are the same as those in Specific Embodiment One, Two, or Three.

[0036] Working Principle

[0037] The support plate 200 is used to store the cell culture plate, and the support plate 200 is hinged to the frame 100.

[0038] The mandrel 300 is rotatably connected to the rectangular frame. The handwheel 310, rubber washer 320, flat washer 330, nut 340 and cam 350 are coaxially installed on the mandrel 300 in sequence. The outer circumferential surface of the cam 350 abuts against the lower surface of the support plate 200. By screwing the handwheel 310, the cam 350 can be driven to rotate, thereby adjusting the angle between the support plate 200 and the frame 100. After the cell culture plate is placed on the support plate 200, the side wall of the cell culture plate abuts against the strip-shaped limiting block 210, preventing the cell culture plate from slipping on the support plate 200.

[0039] The columns of the gantry 230 are slidably connected to the slide rails 220 on the support plate 200. When adding culture medium to different positions of the cell culture plate, the gantry 230 can be slid along the slide rails 220 to the corresponding position, and the outer circumferential surface of the pipette tip is abutted against the groove on the cross beam of the gantry 230 to make the operator's hand more stable.

[0040] The present utility model has been disclosed in the above preferred embodiments, but it is not intended to limit the present utility model. Any person skilled in the art, without departing from the content of the technical solution of the present utility model, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present utility model still fall within the scope of the technical solution of the present utility model.

Claims

1. An auxiliary support for changing liquid in a cell culture plate, comprising a frame (100), the frame (100) comprising a first crossbeam (110), a second crossbeam (120), a first longitudinal beam (130), a second longitudinal beam (140) and four supporting legs, the first crossbeam (110), the second crossbeam (120), the first longitudinal beam (130), and the second longitudinal beam (140) being fixedly connected to form a rectangular frame, the supporting legs being fixedly connected to the rectangular frame, characterized in that: It also includes a support plate (200), a gantry (230) and an angle adjustment unit; The support plate (200) is hinged to the first crossbeam (110), and the support plate (200) is used to store the cell culture plate. A strip-shaped limit block (210) and two slide rails (220) are integrally provided on the upper surface of the support plate (200); The upright posts of the gantry (230) are slidably connected to the slide rail (220), and the upright posts of the gantry (230) are arranged vertically to the support plate (200), and a plurality of grooves for the gun head to abut against are formed on the crossbeam of the gantry (230); The angle adjustment unit comprises a core shaft (300), a hand wheel (310), a rubber washer (320), a flat washer (330), a nut (340) and a cam (350); The core shaft (300) is rotatably connected to the rectangular frame, and the hand wheel (310), the rubber washer (320), the flat washer (330), the nut (340) and the cam (350) are coaxially mounted on the core shaft (300) in sequence, and the hand wheel (310) and the rubber washer (320) are respectively arranged on two side surfaces of the first longitudinal beam (130), and the outer circumferential surface of the cam (350) is against the lower surface of the support plate (200).

2. The auxiliary support for changing liquid in a cell culture plate according to claim 1, characterized in that: The cross section of the slide rail (220) is T-shaped, and a T-shaped slot is provided on the column of the gantry (230), and the T-shaped slot is slidably connected to the slide rail (220).

3. The auxiliary support for changing liquid in a cell culture plate according to claim 2, characterized in that: The crossbeam of the gantry (230) is provided with reinforcing ribs.

4. The auxiliary support for changing liquid in a cell culture plate according to claim 3, characterized in that: The frame (100), the support plate (200), the gantry (230) and the cam (350) are all made of polystyrene.

5. The auxiliary support for changing liquid in a cell culture plate according to claim 4, characterized in that: The core shaft (300) is made of stainless steel.

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