Stem cell exosome sampling device

Abstract

The utility model discloses a stem cell exosome sampling device which comprises a first conveying belt, a second conveying belt and an air cylinder, and a rotary conveying mechanism is arranged between the first conveying belt and the second conveying belt. The air cylinder is located above the rotary conveying mechanism, a lifting frame is installed at the bottom of the air cylinder, and a first driving piece and a rotary table are installed in the horizontal direction of the lifting frame; a plurality of groups of liquid storage pipes are movably mounted on the side wall of the rotary table, a needle tube is mounted at the top end of each group of liquid storage pipes, the needle tubes are communicated with the liquid storage pipes, and a silica gel cross valve is mounted at the tail part of each group of liquid storage pipes; an air pump is mounted on the lifting frame, and an exhaust pipe is connected to the air pump; the rotary table is rotated, the exhaust pipe is inserted into the silica gel cross valve of the liquid storage pipe located on the bottommost side of the rotary table and communicated with an inner cavity of the liquid storage pipe, samples in each sample bottle of the stem cell exosome culture solution can be efficiently and accurately sampled, and time and labor are saved.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a sampling device for stem cell exosomes. Background Art

[0002] Stem cell exosomes are a class of extracellular vesicles with a complete membrane structure secreted by stem cells, containing various bioactive factors such as lipids, proteins, and RNAs, with a diameter of about 30-150 nm. Stem cell exosomes have biological properties similar to those of stem cells. However, compared with stem cells, exosomes have lower immunogenicity, are safer, more stable and efficient, and have high development and application potential in the fields of modern biology and medicine.

[0003] In the process of experimenting with stem cell exosomes, the control variable method is usually used for experiments to observe the experimental results. Many sample reagents need to be sampled one by one, which is inconvenient and inefficient, wasting manpower and time. Summary of the Utility Model

[0004] To solve the technical problems in the background art, the utility model proposes a sampling device for stem cell exosomes.

[0005] A sampling device for stem cell exosomes proposed by the utility model includes a first conveyor belt, a second conveyor belt and a cylinder. The conveying directions of the first conveyor belt and the second conveyor belt are the same and are on the same straight line. A rotary conveying mechanism is arranged between the first conveyor belt and the second conveyor belt;

[0006] The cylinder is located above the rotary conveying mechanism. A lifting frame is installed at the bottom of the cylinder. The cylinder drives the lifting frame to move in the vertical direction. A first driving member and a turntable are installed in the horizontal direction of the lifting frame. The first driving member drives the turntable to rotate, and the axis of the turntable is horizontal;

[0007] A plurality of groups of liquid storage tubes are movably installed on the side wall of the turntable. A syringe needle is installed at the top of each group of liquid storage tubes. The syringe needle is communicated with the liquid storage tube. A silicone cross valve is installed at the tail of each group of liquid storage tubes;

[0008] An air pump is installed on the lifting frame, and an air extraction pipe is connected to the air pump;

[0009] Rotate the turntable, and the air extraction pipe is inserted into the silicone cross valve of the liquid storage tube at the bottommost side of the turntable and communicated with the inner cavity of the liquid storage tube.

[0010] Preferably, the rotary conveying mechanism includes a chassis and a turntable. The turntable is located between the first conveyor belt and the second conveyor belt. The upper surface of the chassis is in the same plane as the upper surfaces of the first conveyor belt and the second conveyor belt. A second driving member and the turntable are installed on the upper surface of the chassis. The second driving member drives the turntable to rotate. A plurality of card slots are formed in the side wall of the turntable for clamping sample bottles.

[0011] Preferably, a plurality of grooves are formed in the side wall of the turntable for movably installing liquid storage tubes.

[0012] Preferably, the tail of the liquid storage tube is threadedly connected to the groove.

[0013] Preferably, the grooves on the side wall of the turntable are distributed centered on the axis of the turntable, and the plurality of card slots on the turntable are distributed centered on the axis of the turntable. The number of grooves on the turntable is the same as the number of card slots on the turntable.

[0014] Preferably, the groove at the bottommost side of the turntable is vertically aligned with one of the card slots on the turntable.

[0015] Preferably, an arc-shaped edge is provided on the chassis, and the arc-shaped edge is in close fit with the side wall of the turntable.

[0016] Preferably, side plates are installed on both sides of the first conveyor belt and the second conveyor belt.

[0017] Preferably, a rubber pad is connected to the inner wall of each card slot.

[0018] In the present utility model, a stem cell exosome sampling device is proposed, which can efficiently and accurately sample the samples in the sample bottles of each stem cell exosome culture solution, saving time and labor.

[0019] When the test tube is on the rotary conveying mechanism, the first driving member drives the turntable to rotate. When the liquid storage tube at the bottommost part of the turntable is in the downward vertical state, it stops. The air extraction tube pushes open the silica gel cross valve and enters the inner cavity of the liquid storage tube. The air cylinder drives the lifting frame to descend. The syringe needle of the liquid storage tube at the bottommost part of the liquid storage tube is inserted into the test tube. The air pump pumps air to extract the sample in the test tube into the liquid storage tube for storage. The air cylinder raises the lifting frame, and the syringe needle leaves the sample tube.

[0020] The first driving member continues to drive the turntable to rotate, and the next liquid storage tube rotates to the bottom side of the turntable. During this process, the previous liquid storage tube rotates out and is no longer at the bottom side of the turntable. As the liquid storage tube rotates, the air extraction pipe falls off from the silica gel cross valve, and again opens the silica gel cross valve in the next liquid storage tube and enters the inner cavity of the next liquid storage tube. At the same time, the rotary conveying mechanism at the bottom also rotates the next sample tube to the bottom of the liquid storage tube. The cylinder descends again, and the syringe needle at the bottom of the liquid storage tube at the bottom of the liquid storage tube is inserted into the test tube, and the air pump pumps air to extract the sample in the test tube into the liquid storage tube for storage. The above operations are repeated until the turntable rotates one week, which means that all the sampling bottles on the turntable are sampled.

[0021] Additional aspects and advantages of the present utility model will be given in part in the following description, will become apparent in part from the following description, or will be understood through the practice of the present utility model. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 is a schematic structural diagram of the present utility model;

[0023] Figure 2 is a top view of the rotary conveying mechanism in the present utility model.

[0024] Figure 3 is a schematic partial structural diagram of the present utility model.

[0025] Figure 4 is a schematic structural diagram of the turntable in the present utility model.

[0026] Description of the reference numerals in the drawings:

[0027] 1, first conveyor belt; 2, rotary conveying mechanism; 201, chassis; 202, arc-shaped edge; 203, card slot; 204, turntable;

[0028] 3, second conveyor belt; 4, cylinder; 5, lifting frame; 6, air pump; 7, air extraction pipe;

[0029] 8, turntable; 801, groove;

[0030] 9, liquid storage tube; 10, syringe needle; 11, silica gel cross valve; 12, side plate. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary only for explaining the present utility model and should not be construed as limiting the present utility model.

[0032] As Figures 1-4A stem cell exosome sampling device shown in the figure includes a first conveyor belt 1, a second conveyor belt 3 and a cylinder 4. The conveying directions of the first conveyor belt 1 and the second conveyor belt 3 are the same and are on the same straight line. A rotary conveying mechanism 2 is provided between the first conveyor belt 1 and the second conveyor belt 3;

[0033] The stem cell exosome culture medium is located in the inner cavity of the sample bottle, is conveyed on the first conveyor belt 1, moves to the second conveying mechanism after passing through the rotary conveying mechanism 2, and is continuously conveyed by the second mechanism.

[0034] The cylinder 4 is located above the rotary conveying mechanism 2. A lifting frame 5 is installed at the bottom of the cylinder 4. The cylinder 4 drives the lifting frame 5 to move in the vertical direction. A first driving member and a turntable 8 are installed in the horizontal direction of the lifting frame 5. The first driving member drives the turntable 8 to rotate, and the axis of the turntable 8 is a horizontal line;

[0035] A plurality of groups of liquid storage tubes 9 are movably installed on the side wall of the turntable 8. A syringe needle 10 is installed at the top of each group of liquid storage tubes 9. The syringe needle 10 is communicated with the liquid storage tube 9. A silica gel cross valve 11 is installed at the tail of each group of liquid storage tubes 9;

[0036] An air pump 6 is installed on the lifting frame 5. An air extraction pipe 7 is connected to the air pump 6;

[0037] Rotate the turntable 8, and the air extraction pipe 7 is inserted into the silica gel cross valve 11 of the liquid storage tube 9 at the bottommost side of the turntable 8 and is communicated with the inner cavity of the liquid storage tube 9.

[0038] When the test tube is on the rotary conveying mechanism 2, the first driving member drives the turntable 8 to rotate. When the liquid storage tube 9 at the bottommost part of the turntable 8 is in the downward vertical state, it stops. The air extraction pipe 7 pushes open the silica gel cross valve 11 and enters the inner cavity of the liquid storage tube 9. The cylinder 4 drives the lifting frame 5 to descend. The syringe needle 10 of the liquid storage tube 9 at the bottommost part of the liquid storage tube 9 is inserted into the test tube. The air pump 6 extracts air, extracts the sample in the test tube into the liquid storage tube 9 for storage, and the cylinder 4 raises the lifting frame 5, and the syringe needle 10 leaves the sample tube;

[0039] The first driving member continues to drive the turntable 8 to rotate, and the next liquid storage tube 9 rotates to the bottom side of the turntable 8. During this process, the previous liquid storage tube 9 rotates out and is no longer at the bottom side of the turntable 8. As the liquid storage tube 9 rotates, the air extraction pipe 7 detaches from the silicone cross valve 11, and then opens the silicone cross valve 11 in the next liquid storage tube 9 again and enters the inner cavity of the next liquid storage tube 9. At the same time, the rotary conveying mechanism 2 at the bottom also rotates the next sample tube to the bottom of this liquid storage tube 9. The cylinder 4 descends again, and the syringe needle 10 at the bottom of the liquid storage tube 9 of the liquid storage tube 9 is inserted into the test tube. The air pump 6 pumps air to extract the sample in the test tube into the liquid storage tube 9 for storage. The above operations are cycled until the turntable 8 rotates one week, which means that all the sampling bottles on the turntable 8 are sampled.

[0040] Preferably, the rotary conveying mechanism 2 includes a chassis 201 and a turntable 204. The turntable 204 is located between the first conveyor belt 1 and the second conveyor belt 3. The upper surface of the chassis 201 is on the same plane as the upper surfaces of the first conveyor belt 1 and the second conveyor belt 3. A second driving member and the turntable 204 are installed on the upper surface of the chassis 201. The second driving member drives the turntable 204 to rotate. Multiple groups of card slots 203 are formed on the side wall of the turntable 204 for clamping sample bottles.

[0041] The sample bottle is conveyed to the chassis 201 by the first conveyor belt 1 and clamped onto the card slot 203 of the turntable 204. The second driving member drives the turntable 204 to rotate, and the turntable 204 drives the sample bottle in the card slot 203 to rotate.

[0042] Preferably, multiple groups of grooves 801 are formed on the side wall of the turntable 8 for movably installing the liquid storage tube 9.

[0043] Preferably, the tail of the liquid storage tube 9 is threadedly connected to the groove 801.

[0044] The groove 801 is used to facilitate the disassembly and assembly of the liquid storage tube 9. When the sampling is full, the liquid storage tube 9 can be removed and a batch of new liquid storage tubes 9 can be installed.

[0045] Preferably, the grooves 801 on the side wall of the turntable 8 are distributed centered on the axis of the turntable 8, and the multiple groups of card slots 203 on the turntable 204 are distributed centered on the axis of the turntable 204. The number of grooves 801 on the turntable 8 is the same as the number of card slots 203 on the turntable 204.

[0046] It is only necessary that the first driving member drives the turntable 8 to rotate by the same angle.

[0047] Preferably, the groove 801 at the bottom side of the turntable 8 is vertically aligned with one of the card slots 203 on the turntable 204, which can ensure that the syringe needle 10 on the liquid storage tube 9 is inserted into the sample tube.

[0048] Preferably, an arc-shaped edge 202 is provided on the chassis 201, and the arc-shaped edge 202 is in close fit with the side wall of the turntable 204, which can limit the sample bottle in the card slot 203 and prevent it from falling out of the card slot 203.

[0049] Preferably, side plates 12 are installed on both sides of the first conveyor belt 1 and the second conveyor belt 3, which can play a protective role during the transportation of the sample bottles.

[0050] Preferably, a rubber pad is connected to the inner wall of each card slot 203 to further protect the side wall of the sample bottle.

[0051] It should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0052] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present invention, the meaning of "a plurality" is at least two, such as two, three, etc., unless otherwise specifically defined.

[0053] In the present invention, unless otherwise clearly defined and limited, the terms "install", "connect", "connection", "fix", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection, an electrical connection, or communication with each other; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the internal connection of two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0054] In the present utility model, unless otherwise clearly stipulated and defined, the first feature being "on" or "under" the second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply indicates that the first feature has a lower horizontal height than the second feature.

[0055] The above is only a preferred specific embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution of the present utility model and its inventive concept, makes equivalent substitutions or changes, and all should be covered by the protection scope of the present utility model.

Claims

1. A stem cell exosome sampling device, comprising a first conveyor belt (1), a second conveyor belt (3) and a cylinder (4), characterized in that: The first conveyor belt (1) and the second conveyor belt (3) have the same conveying direction and are on the same straight line, and a rotating conveying mechanism (2) is provided between the first conveyor belt (1) and the second conveyor belt (3); The cylinder (4) is located above the rotating conveying mechanism (2), a lifting frame (5) is installed at the bottom of the cylinder (4), the cylinder (4) drives the lifting frame (5) to move in the vertical direction, a first driving member and a turntable (8) are installed in the horizontal direction of the lifting frame (5), the first driving member drives the turntable (8) to rotate, and the axis of the turntable (8) is a horizontal line; A plurality of groups of liquid storage tubes (9) are movably mounted on the side wall of the turntable (8), a needle tube (10) is mounted at the top end of each group of liquid storage tubes (9), the needle tube (10) and the liquid storage tube are connected to each other, and a silicone cross valve (11) is mounted at the tail end of each group of liquid storage tubes (9); An air pump (6) is installed on the lifting frame (5), and an air extraction pipe (7) is connected to the air pump (6); The turntable (8) is rotated, and the air extraction pipe (7) is inserted into the silicone cross valve (11) of the liquid storage pipe (9) located at the bottom side of the turntable (8) and communicated with the inner cavity of the liquid storage pipe (9).

2. A stem cell exosome sampling device according to claim 1, characterized in that: The rotary conveying mechanism (2) comprises a chassis (201) and a turntable (204); the turntable (204) is located between the first conveyor belt (1) and the second conveyor belt (3); the upper surface of the chassis (201) is located in the same plane as the upper surfaces of the first conveyor belt (1) and the second conveyor belt (3); a second driving member and a turntable (204) are installed on the upper surface of the chassis (201); the second driving member drives the turntable (204) to rotate; and a plurality of groups of card slots (203) are provided on the side wall of the turntable (204) for card receiving sample bottles.

3. A stem cell exosome sampling device according to claim 2, characterized in that: The side wall of the turntable (8) is provided with a plurality of grooves (801) for movably mounting the liquid storage tube (9).

4. A stem cell exosome sampling device according to claim 3, characterized in that: The tail of the liquid storage tube (9) is connected to the groove (801) via threads.

5. A stem cell exosome sampling device according to claim 4, characterized in that: The grooves (801) are distributed on the side wall of the turntable (8) at the center of the axis of the turntable (8), and the multiple groups of slots (203) are distributed on the turntable (204) at the center of the axis of the turntable (204), and the number of the grooves (801) on the turntable (8) is the same as the number of the slots (203) on the turntable (204).

6. A stem cell exosome sampling device according to claim 5, characterized in that: The groove (801) located at the bottom of the turntable (8) is vertically opposite to one of the slots (203) on the turntable (204).

7. A stem cell exosome sampling device according to claim 2, characterized in that: The bottom plate (201) is provided with an arc-shaped rib (202), and the arc-shaped rib (202) is tightly fitted with the side wall of the rotating disk (204).

8. The stem cell exosome sampling device according to claim 1, characterized in that: Side plates (12) are installed on both sides of the first conveyor belt (1) and the second conveyor belt (3).

9. A stem cell exosome sampling device according to claim 2, characterized in that: The inner wall of each slot (203) is connected to a rubber pad.

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