Sample adding device for fluorescent quantitative PCR (Polymerase Chain Reaction) instrument

Abstract

The utility model discloses a sample adding device for a fluorescent quantitative PCR instrument, which comprises a sample adding table, an equipment frame is fixedly connected to the center of the top of the sample adding table, belt pulleys are arranged at the two ends of the top of the sample adding table, the outer walls of the two belt pulleys are jointly connected with a conveying belt through a belt, and the outer side wall of the conveying belt is fixedly connected with a storage mechanism. A driving motor corresponding to the belt pulley is fixedly connected to one end of the top of the equipment frame, and a transverse frame is fixedly connected to the center of the top of the equipment frame. According to the quantitative sample adding device, the object placing mechanism and the material adding mechanism are matched with each other, a sample to be added is pumped into the quantitative cylinder through the piston, the sample is directly pushed into a PCR tube during sample adding, the total quantity of pre-loaded samples in the quantitative cylinder is visual and transparent in the process, and even if multiple groups of different quantities of samples are added at the same time, the samples are added through the outside of the quantitative cylinder. A worker still can visually monitor the sample adding condition, so that the worker can better control the sample adding process of the PCR tube.

Description

Technical Field

[0001] The utility model relates to the technical field of PCR tube sampling equipment, and specifically relates to a sample adding device for a fluorescence quantitative PCR instrument. Background Technique

[0002] A PCR instrument, also known as a gene amplification instrument, is mainly used for gene amplification in scientific research and clinical applications, such as qualitative PCR gene amplification, fluorescence / enzyme immunoassay endpoint quantitative DNA gene amplification, gene chip and other gene analysis applications. It is applicable to various domestic and foreign PCR reagent kits for infectious diseases (various hepatitis viruses, Mycobacterium tuberculosis, STDs, prenatal and postnatal care, mycoplasma, chlamydia, parasites, etc.), tumors (P53, Helicobacter pylori, etc.), and scientific research (genetic identification, bacterial and viral typing, etc.).

[0003] Currently, when the existing PCR instruments perform fluorescence quantitative sample addition, most of them use a pipette to inject the fluorescence sample into the PCR tube, and its injection volume is controlled by an external control module, and the injection volume of the sample is not intuitive. Often during the experiment, multiple groups of samples need to be extracted. Under the requirements of the control variable method, the amount of the total sample is also one of the variables. When facing multiple groups of quantitative injection data, it is more difficult for the staff to control the sample addition process of the PCR instrument. When the sample injection is completed, it is found that the sample injection is excessive or lacking, which is likely to cause waste of sample resources. Content of the Utility Model

[0004] The purpose of this part is to outline some aspects of the implementation mode of the utility model and briefly introduce some preferred implementation modes. Simplifications or omissions may be made in this part, as well as in the abstract of the specification and the title of the utility model of this application, to avoid obscuring the purpose of this part, the abstract of the specification, and the title of the utility model. However, such simplifications or omissions shall not be used to limit the scope of the utility model.

[0005] To solve the above technical problems, according to one aspect of the utility model, the following technical solutions are provided by the utility model:

[0006] A sample adding device for a fluorescence quantitative PCR instrument, including a sample adding platform, a device rack is fixedly connected to the center of the top of the sample adding platform, belt pulleys are arranged at both ends of the top of the sample adding platform, a conveying belt is belt-connected to the outer walls of the two belt pulleys, a placing mechanism is fixedly connected to the outer side wall of the conveying belt, a driving motor corresponding to the belt pulley is fixedly connected to one end of the top of the device rack, a cross rack is fixedly connected to the center of the top of the device rack, hydraulic rods are fixedly connected to the tops of both ends of the cross rack, and a feeding mechanism is arranged at the bottom of the telescopic end of the hydraulic rod passing through the cross rack;

[0007] The storage mechanism includes a storage seat, a tray is slidably connected to the inner wall of the storage seat, a spring is fixedly connected between the tray and the inner bottom of the storage seat, and a protective strip is fixedly connected to the outer wall of the storage seat near the top;

[0008] The feeding mechanism includes a connecting frame fixedly connected to the bottom of the telescopic end of the hydraulic rod. An annular frame is fixedly connected to the outer end of the connecting frame. A metering cylinder is arranged inside the annular frame. An electric push rod is arranged on the top of the metering cylinder. A piston is fixedly connected to the telescopic end of the electric push rod. A first one-way valve and a second one-way valve are fixedly connected to the bottom of the metering cylinder. The first one-way valve and the second one-way valve are respectively fixedly connected with an injection tube and a filling hose at the bottom.

[0009] As a preferred scheme of a sample adding device for a fluorescence quantitative PCR instrument according to the present invention, the driving shaft of the driving motor penetrates through the equipment frame and is fixedly connected to the center of the axis of the belt pulley. The center of the bottom of the belt pulley is connected to the top of the sample adding table through a bearing. By driving the belt pulley to rotate by the driving motor, the conveying belt is driven to move, so as to convey the PCR tube and facilitate cyclic feeding.

[0010] As a preferred scheme of a sample adding device for a fluorescence quantitative PCR instrument according to the present invention, a limiting ring is fixedly connected between the protective strip and the inner wall of the storage seat. The limiting ring includes a sponge ring, and the outside of the sponge ring is wrapped with cloth. When the PCR tube is placed on the top of the tray, the limiting ring is convenient for positioning the outside of the PCR tube and ensuring its stability during the conveying process.

[0011] As a preferred scheme of a sample adding device for a fluorescence quantitative PCR instrument according to the present invention, a support ring is fixedly connected to the outer side wall of the metering cylinder. The outer side wall of the metering cylinder is threadedly connected to the inner wall of the annular frame and can rotate in the annular frame. The metering cylinder can be flexibly disassembled for maintenance. When installing, when it is screwed until the support ring contacts the annular frame, it indicates that the installation is in place.

[0012] As a preferred scheme of a sample adding device for a fluorescence quantitative PCR instrument according to the present invention, a top cover is threadedly connected to the top of the metering cylinder. The bottom of the electric push rod is fixedly connected to the top cover. The telescopic end of the electric push rod penetrates through the top cover and extends into the metering cylinder. The piston is in close fit with the inner wall of the metering cylinder. By driving the piston to move in the metering cylinder by the electric push rod, it is convenient to quantitatively push the previously inhaled fluorescence sample into the PCR tube.

[0013] As a preferred solution of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model, the first one-way valve opens outward from the quantitative cylinder, and the second one-way valve opens inward into the quantitative cylinder. By driving the piston to rise in the quantitative cylinder with an electric push rod, the sample can be quantitatively sucked into the quantitative cylinder through the second one-way valve. The rising height of the piston is proportional to the total amount of the sample. When injecting, driving the piston to descend can inject the quantitative sample into the PCR tube through the injection tube.

[0014] As a preferred solution of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model, precise scale lines are provided on the outer side wall of the quantitative cylinder, which is convenient for the staff to intuitively master the sample injection volume during inspections.

[0015] As a preferred solution of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model, the center of the bottom of the injection tube and the center of the tray are located in the same vertical plane. When the PCR tube is placed on the tray, the injection tube corresponds to the middle of the tube body, which is convenient for injecting the quantitative sample into the tube through the injection tube.

[0016] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0017] The present utility model mutually cooperates between the placing mechanism and the feeding mechanism, sucks the sample to be added into the quantitative cylinder through the piston, and directly pushes the sample into the PCR tube when adding the sample. The total amount of the pre-installed sample in the quantitative cylinder is intuitive and transparent during the process. Even when adding multiple groups of samples with different total amounts simultaneously, the staff can still intuitively monitor the sample addition status through the outside of the quantitative cylinder, which is convenient for the staff to better control the sample adding process in the PCR tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the present utility model will be described in detail below in conjunction with the drawings and detailed embodiments. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts. Among them:

[0019] Figure 1 is a three-dimensional view of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model;

[0020] Figure 2 is a schematic diagram of the placing mechanism of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model;

[0021] Figure 3 is a schematic diagram of the feeding mechanism of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model;

[0022] Figure 4 Schematic diagram of the annular frame structure of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model;

[0023] Figure 5 Schematic diagram of the quantitative cylinder structure of a sample adding device for a fluorescence quantitative PCR instrument according to the present utility model.

[0024] Legend: 1. Sample adding table; 2. Equipment frame; 3. Pulley; 4. Conveyor belt; 5. Driving motor; 6. Horizontal frame; 7. Hydraulic rod; 8. Placing mechanism; 801. Placing seat; 802. Tray; 803. Spring; 804. Protective strip; 9. Feeding mechanism; 901. Connecting frame; 902. Annular frame; 903. Quantitative cylinder; 904. Electric push rod; 905. Piston; 906. First one-way valve; 907. Second one-way valve; 908. Injection tube; 909. Filler hose; 10. Limit ring; 11. Support ring; 12. Top cover. Detailed implementation manners

[0025] In order to make the above objects, features and advantages of the present utility model more obvious and understandable, the following will describe in detail the specific implementation manners of the present utility model with reference to the accompanying drawings.

[0026] Secondly, the present utility model will be described in detail with reference to the schematic diagrams. When detailing the implementation manners of the present utility model, for the convenience of description, the cross-sectional views showing the device structure will be enlarged locally not in accordance with the general scale, and the schematic diagrams are only examples and should not limit the scope of protection of the present utility model herein. In addition, in actual production, three-dimensional spatial dimensions including length, width and depth should be included.

[0027] In order to make the purpose, technical solution and advantages of the present utility model clearer, the following will further describe in detail the implementation manners of the present utility model with reference to the accompanying drawings.

[0028] Please refer to Figures 1-5 , the present utility model provides a sample adding device for a fluorescence quantitative PCR instrument, including a sample adding table 1, a device frame 2 is fixedly connected to the center of the top of the sample adding table 1, pulley 3s are arranged at both ends of the top of the sample adding table 1, a conveyor belt 4 is belt-connected to the outer walls of the two pulley 3s, a driving shaft of a driving motor 5 penetrates through the device frame 2 and is fixedly connected to the center of the axis of the pulley 3, and the center of the bottom of the pulley 3 is rotationally connected to the top of the sample adding table 1. By driving the pulley 3 to rotate through the driving motor 5, the conveyor belt 4 is driven to move, so as to convey the PCR tube and facilitate cyclic feeding.

[0029] A placing mechanism 8 is fixedly connected to the outer side wall of the conveyor belt 4. One end of the top of the equipment frame 2 is fixedly connected with a driving motor 5 corresponding to the belt pulley 3. The center of the top of the equipment frame 2 is fixedly connected with a cross frame 6. Hydraulic rods 7 are fixedly connected to the tops of both ends of the cross frame 6. The telescopic ends of the hydraulic rods 7 penetrate through the cross frame 6 from the bottom and are provided with a feeding mechanism 9.

[0030] The placing mechanism 8 includes a placing seat 801. A tray 802 is slidably connected to the inner wall of the placing seat 801. A spring 803 is fixedly connected between the tray 802 and the inner bottom of the placing seat 801.

[0031] A protection strip 804 is fixedly connected to the outer wall of the placing seat 801 near the top. Among them, a limiting ring 10 is fixedly connected between the protection strip 804 and the inner wall of the placing seat 801. The limiting ring 10 includes a sponge ring, and the outside of the sponge ring is wrapped with cloth. When the PCR tube is placed on the top of the tray 802, it is convenient to position the outside of the PCR tube through the limiting ring 10 to ensure its stability during the conveying process.

[0032] The feeding mechanism 9 includes a connecting frame 901 fixedly connected to the bottom of the telescopic end of the hydraulic rod 7. An annular frame 902 is fixedly connected to the outer end of the connecting frame 901. A metering cylinder 903 is arranged inside the annular frame 902. A support ring 11 is fixedly connected to the outer side wall of the metering cylinder 903. The outer side wall of the metering cylinder 903 is threadedly connected to the inner wall of the annular frame 902 and can rotate in the annular frame 902. The metering cylinder 903 can be flexibly disassembled for maintenance. When installing, when it is screwed until the support ring 11 contacts the annular frame 902, it indicates that the installation is in place.

[0033] In this embodiment, precise graduation lines are arranged on the outer side wall of the metering cylinder 903, which is convenient for the staff to intuitively master the sample injection volume during inspection.

[0034] An electric push rod 904 is arranged on the top of the metering cylinder 903. A piston 905 is fixedly connected to the telescopic end of the electric push rod 904. A top cover 12 is threadedly connected to the top of the metering cylinder 903. The bottom of the electric push rod 904 is fixedly connected to the top cover 12. The telescopic end of the electric push rod 904 penetrates through the top cover 12 and extends into the metering cylinder 903. The piston 905 is in close fit with the inner wall of the metering cylinder 903. By driving the piston 905 to move in the metering cylinder 903 through the electric push rod 904, it is convenient to quantitatively push the fluorescent sample previously inhaled into the metering cylinder 903 into the PCR tube.

[0035] At the bottom of the measuring cylinder 903, a first one-way valve 906 and a second one-way valve 907 are fixedly connected. At the bottoms of the first one-way valve 906 and the second one-way valve 907, an injection tube 908 and a packing hose 909 are respectively fixedly connected. The center of the bottom of the injection tube 908 and the center of the tray 802 are located in the same vertical plane. When the PCR tube is placed on the tray 802, the injection tube 908 corresponds to the middle of the tube body, facilitating the injection of the measured sample into the tube through the injection tube 908.

[0036] The first one-way valve 906 opens outward from the measuring cylinder 903, and the second one-way valve 907 opens inward to the measuring cylinder 903. By using the electric push rod 904 to drive the piston 905 to rise in the measuring cylinder 903, the sample can be quantitatively inhaled into the measuring cylinder 903 through the second one-way valve 907. The height of the piston 905 rising is proportional to the total amount of the sample. When injecting, drive the piston 905 to descend, and the measured sample can be injected into the PCR tube through the injection tube 908.

[0037] During use, connect the packing hose 909 to the external sample. The sample can be transported and supplied through the packing hose 909. Insert the PCR tube from the top of the limit ring 10 to the top of the tray 802 and place it. Start the drive motor 5 to drive the pulley 3 to rotate, drive the conveyor belt 4 to convey a plurality of placing seats 801, and make the placing seats 801 move past the bottom of the injection tube 908 in sequence.

[0038] Use an external PLC program to achieve precise control of the stroke of the telescopic end of the electric push rod 904. When the electric push rod 904 drives the piston 905 to rise, the sample is inhaled into the measuring cylinder 903 through the second one-way valve 907. The height of the piston 905 rising is proportional to the total amount of the sample, facilitating the control of the total amount of the sample and realizing quantitative sample addition. When injecting, the electric push rod 904 drives the piston 905 to descend, and the measured sample can be injected into the PCR tube through the injection tube 908.

[0039] In this embodiment, three measuring cylinders 903 and corresponding placing seats 801 are provided. The stroke of the telescopic ends of the electric push rods 904 at the tops of the measuring cylinders 903 are independent of each other, facilitating the simultaneous addition of different amounts of samples into multiple measuring cylinders 903 respectively. During the sample inhalation stage of the measuring cylinder 903, the staff can visually observe the differences in the total amount of the samples, making the sample amount in the entire sample addition process easier to control. When the sample supply is insufficient or the sample addition data is incorrect, resulting in an abnormal sample addition amount, the height of the sample column in the corresponding measuring cylinder 903 changes. At this time, the staff can quickly discover and replenish the material in time to prevent the same error from occurring in subsequent sample addition, so as to avoid greater losses.

[0040] Although the present utility model has been described above with reference to the embodiments, various improvements can be made thereto and components thereof can be replaced with equivalents without departing from the scope of the present utility model. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed by the present utility model can be combined with each other in any way, and the exhaustive description of these combinations is not given in this specification only for the consideration of saving space and resources. Therefore, the present utility model is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A sample loading device for a fluorescence quantitative PCR instrument, comprising a sample loading platform (1), characterized in that: The top center of the loading platform (1) is fixedly connected to an equipment frame (2), both ends of the top of the loading platform (1) are provided with pulleys (3), the outer walls of the two pulleys (3) are connected to a conveyor belt (4) through a common belt, the outer wall of the conveyor belt (4) is fixedly connected to a storage mechanism (8), one end of the top of the equipment frame (2) is fixedly connected to a driving motor (5) corresponding to the pulley (3), the top center of the equipment frame (2) is fixedly connected to a cross frame (6), the tops of both ends of the cross frame (6) are fixedly connected to hydraulic rods (7), the bottom of the telescopic end of the hydraulic rod (7) passes through the cross frame (6) and is provided with a feeding mechanism (9); The storage mechanism (8) comprises a storage seat (801), the inner wall of the storage seat (801) is slidably connected to a tray (802), a spring (803) is fixedly connected between the tray (802) and the inner bottom of the storage seat (801), and a guard strip (804) is fixedly connected to the outer wall of the storage seat (801) near the top; The feeding mechanism (9) comprises a connecting frame (901) fixedly connected to the bottom of the telescopic end of the hydraulic rod (7); the outer end of the connecting frame (901) is fixedly connected to an annular frame (902); a quantitative cylinder (903) is arranged inside the annular frame (902); an electric push rod (904) is arranged on the top of the quantitative cylinder (903); the telescopic end of the electric push rod (904) is fixedly connected to a piston (905); the bottom of the quantitative cylinder (903) is fixedly connected to a first one-way valve (906) and a second one-way valve (907); the bottoms of the first one-way valve (906) and the second one-way valve (907) are fixedly connected to an injection tube (908) and a filling hose (909), respectively.

2. A sample adding device for a fluorescence quantitative PCR instrument according to claim 1, characterized in that: The driving shaft of the driving motor (5) passes through the equipment frame (2) and is fixedly connected to the axis of the belt pulley (3); the bottom center of the belt pulley (3) is connected to the top bearing of the sample loading platform (1).

3. A sample adding device for fluorescent quantitative PCR instrument according to claim 1, characterized in that: The protective strip (804) and the inner wall of the storage seat (801) are fixedly connected to a limiting ring (10), and the limiting ring (10) comprises a sponge ring, and the outside of the sponge ring is wrapped with cloth.

4. A sample adding device for fluorescent quantitative PCR instrument according to claim 1, characterized in that: The outer wall of the quantitative cylinder (903) is fixedly connected to a support ring (11), and the outer wall of the quantitative cylinder (903) is threadedly connected to the inner wall of the annular frame (902).

5. The sample adding device for fluorescent quantitative PCR instrument according to claim 1, characterized in that: The top of the quantitative cylinder (903) is threadedly connected to a top cover (12), the bottom of the electric push rod (904) is fixedly connected to the top cover (12), the telescopic end of the electric push rod (904) passes through the top cover (12) and extends into the interior of the quantitative cylinder (903), and the piston (905) is tightly fitted to the inner wall of the quantitative cylinder (903).

6. The sample adding device for fluorescent quantitative PCR instrument according to claim 1, characterized in that: The first one-way valve (906) opens toward the outside of the metering cylinder (903), and the second one-way valve (907) opens toward the inside of the metering cylinder (903).

7. The sample adding device for fluorescent quantitative PCR instrument according to claim 1, characterized in that: The outer side wall of the quantitative cylinder (903) is provided with precise scale lines.

8. The sample adding device for fluorescent quantitative PCR instrument according to claim 1, characterized in that: The center of the bottom of the injection tube (908) and the center of the tray (802) are located in the same vertical plane.

Images