96-hole device for incubating cells in water bath environment

Abstract

The utility model discloses a 96-hole device for incubating cells in a water bath environment, which comprises a hole plate and a water blocking cover covered on the top surface of the hole plate, a plurality of accommodating shells are integrally formed at the bottom of the hole plate, 96 holes are formed in the inner spaces of the accommodating shells, and the water blocking cover is covered on the top surface of the hole plate. A plurality of rubber plugs are integrally formed on the inner side face of the water blocking cover. According to the utility model, the water blocking cover with the plurality of rubber plugs is mounted on the top surface of the pore plate in a covering manner, and the plurality of rubber plugs of the water blocking cover are respectively inserted into the plurality of accommodating shells of the pore plate by mounting the water blocking cover on the top surface of the pore plate in a covering manner, so that 96 pores formed by the plurality of accommodating shells on the top surface of the pore plate are covered and sealed; on one hand, cell sample pollution caused by falling of water drops in the water bath stage is avoided, on the other hand, the water blocking cover is kept to be installed and reliable in a water blocking cover covering and rubber plug sealing mode, the water blocking cover is easy to dismantle quickly in the later period, and the situation that detection cannot be conducted due to cell sample pouring is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, and specifically, to a 96-well device for incubating cells in a water bath environment. Background Technique

[0002] The 96-well plate is made by a special process. The 96-well plate is mainly used for storing samples, processing samples and sampling operations. The 96-well plate has many and dense holes, the plate body is made of transparent material, and the sizes and appearances of the holes are the same. The 96-well plate is a commonly used consumable in biological experiments, mainly used for tests such as PCR and fluorescence quantitative PCR.

[0003] After retrieval, a 96-well plate with the publication number CN219129305U belongs to the technical field of medical devices. It includes a well plate. A plurality of first through holes are opened on the upper surface of the well plate. A cover plate is arranged above the well plate. A plurality of second through holes are opened on the cover plate. The first through holes and the second through holes are aligned one by one. Two baffles are slidably connected to the cover plate at the second through holes. The two baffles are arranged in contact with each other on the adjacent sides. A moving device for controlling the baffles to move in the direction away from each other is arranged above the baffles. This application has the effect of reducing the operation errors of staff.

[0004] The above solution has the effect of reducing the operation errors of staff. However, the current 96-well plate still has the following problems during the water bath heating stage: If a lid is set on the 96-well plate in a 37°C water bath pot, water droplets are likely to form and fall in, resulting in sample contamination. If the 96-well plate is sealed too tightly, when it is disassembled, the sample is likely to spill and cannot be detected.

[0005] Regarding the problems in the related technology, no effective solution has been proposed yet. Content of the Utility Model

[0006] (1) Technical Problems to be Solved

[0007] To solve the problems raised above, the utility model provides a 96-well device for incubating cells in a water bath environment, thereby solving the problems in the above background technique.

[0008] (2) Technical Solutions

[0009] To achieve the problems raised above, the specific technical solutions adopted by the utility model are as follows:

[0010] A 96-well device for incubating cells in a water bath environment, comprising a well plate and a water-blocking cover covered on the top surface thereof. A plurality of accommodating shells are integrally formed at the bottom of the well plate, and the internal spaces of the plurality of accommodating shells form 96 wells. A water-blocking cover is covered and installed on the top surface of the well plate, and a plurality of rubber plugs are integrally formed on the inner side surface of the water-blocking cover, and the plurality of rubber plugs are respectively inserted into the inner sides of the plurality of accommodating shells. Positioning sleeves are integrally formed at the four corners of the bottom surface of the well plate, and a steel handle is arranged above the well plate. A U-shaped support member is welded to each end of the steel handle, and positioning columns are respectively welded to the two ends of the U-shaped support member corresponding to the positioning sleeves, and the positioning columns of the U-shaped support member are inserted into the positioning sleeves.

[0011] Further, a plurality of rectangular protrusions are integrally injection-molded on the top surface of the water-blocking cover, and two convex rings are respectively arranged on each rubber plug of the water-blocking cover.

[0012] Further, a rubber sleeve is sleeved on the middle part of the steel handle, and a grid-shaped anti-slip pattern is arranged on the surface of the rubber sleeve.

[0013] Further, the water-blocking cover is integrally made of transparent silica gel, and the size of the water-blocking cover is adapted to the size of the well plate.

[0014] Further, the accommodating shell is composed of a cylindrical part and a conical part, and the cylindrical part is above the conical part, and the size of the rubber plug is matched with the size of the inner space of the cylindrical part of the accommodating shell.

[0015] Further, the water-blocking cover is integrally of a rectangular structure, and the border of the water-blocking cover is in contact with the side surface of the well plate.

[0016] (III) Beneficial effects

[0017] Compared with the prior art, the present utility model provides a 96-well device for incubating cells in a water bath environment, which has the following beneficial effects:

[0018] (1). In the present utility model, a water-blocking cover with a plurality of rubber plugs is covered and installed on the top surface of the well plate. By covering and installing the water-blocking cover on the top surface of the well plate, the plurality of rubber plugs of the water-blocking cover are respectively inserted and installed into the plurality of accommodating shells of the well plate, so that the 96 wells formed by the plurality of accommodating shells on the top surface of the well plate are covered and sealed. On the one hand, it avoids the water droplets falling during the water bath stage from causing cell sample contamination. On the other hand, the method of covering the water-blocking cover and sealing with rubber plugs is used to maintain the installation and reliability of the water-blocking cover, and it is easy to quickly remove the water-blocking cover in the later stage, avoiding the situation that the cell sample spills and cannot be detected.

[0019] (2) The present utility model adopts a steel handle with U-shaped supports at both ends. By placing the U-shaped supports at both ends of the steel handle under the orifice plate, the positioning columns of the two U-shaped supports are respectively inserted into the positioning sleeves arranged at the four corners of the bottom surface of the orifice plate, achieving the purpose of facilitating the indirect loading and unloading of the orifice plate. Compared with the existing orifice plate, it can avoid the situation of cell contamination caused by direct contact during loading and unloading of the orifice plate, thus ensuring the accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.

[0021] Figure 1 FIG. 9 is a schematic structural diagram of a 96-well device for incubating cells in a water bath environment proposed by the present utility model;

[0022] Figure 2 FIG. 13 is a schematic structural diagram of the water-blocking cover of the present utility model;

[0023] Figure 3 FIG. 17 is a bottom view of the orifice plate of the present utility model;

[0024] Figure 4 FIG. 21 is a schematic structural diagram of the steel handle of the present utility model.

[0025] In the figure:

[0026] 1, orifice plate; 2, water-blocking cover; 3, accommodating shell; 4, rubber plug; 5, steel handle; 6, positioning sleeve; 7, U-shaped support; 8, positioning column; 9, convex ring; 10, rubber sleeve. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0027] To further illustrate the embodiments, the present utility model provides drawings. These drawings are a part of the disclosure of the present utility model, mainly used to illustrate the embodiments and can cooperate with the relevant descriptions in the specification to explain the operating principle of the embodiments. With reference to these contents, those of ordinary skill in the art should be able to understand other possible implementation manners and the advantages of the present utility model. The components in the figures are not drawn to scale, and similar component symbols are usually used to represent similar components.

[0028] According to the embodiments of the present utility model, a 96-well device for incubating cells in a water bath environment is provided.

[0029] Now, the present utility model will be further described in combination with the drawings and specific embodiments, as Figures 1-4As shown in the figure, a 96-well device for incubating cells in a water bath environment according to an embodiment of the present invention includes a well plate 1 and a water-blocking cover 2 covered on the top surface thereof. A plurality of accommodating shells 3 are integrally formed at the bottom of the well plate 1, and the internal spaces of the plurality of accommodating shells 3 form 96 wells. A water-blocking cover 2 is covered and installed on the top surface of the well plate 1, and a plurality of rubber plugs 4 are integrally formed on the inner side surface of the water-blocking cover 2. The plurality of rubber plugs 4 are respectively inserted into the inner sides of the plurality of accommodating shells 3. Positioning sleeves 6 are integrally formed at the four corners of the bottom surface of the well plate 1. A steel handle 5 is arranged above the well plate 1. Two U-shaped support members 7 are respectively welded at both ends of the steel handle 5. Positioning columns 8 are respectively welded at both ends of the U-shaped support member 7 corresponding to the positioning sleeves 6. The positioning columns 8 of the U-shaped support member 7 are inserted into the positioning sleeves 6. Among them, the plurality of rubber plugs 4 of the water-blocking cover 2 correspond to the plurality of accommodating shells 3 of the well plate 1 one by one. The well plate 1 of this device has the same structure as the existing well plate 1. For the parts of the well plate 1 not involved in this device, they are the same as the prior art or can be implemented by the prior art.

[0030] In one embodiment, a plurality of rectangular protrusions are integrally injection-molded on the top surface of the water-blocking cover 2, and two convex rings 9 are respectively arranged on each rubber plug 4 of the water-blocking cover 2. Through the settings of the rubber plug 4 and the convex ring 9, it is possible to prevent sample contamination caused by water vapor entering the accommodating shell 3.

[0031] In one embodiment, a rubber sleeve 10 is sleeved on the middle part of the steel handle 5, and a grid-shaped anti-slip pattern is arranged on the surface of the rubber sleeve 10. Through the setting of the rubber sleeve 10, the comfort of using the steel handle 5 can be increased.

[0032] In one embodiment, the water-blocking cover 2 is entirely made of transparent silica gel, and the size of the water-blocking cover 2 is adapted to the size of the well plate 1. Through the water-blocking cover 2 made of transparent silica gel material, it is easy to load and unload the water-blocking cover 2.

[0033] In one embodiment, the accommodating shell 3 is composed of a cylindrical part and a conical part, and the cylindrical part is above the conical part. The size of the rubber plug 4 matches the size of the inner space of the cylindrical part of the accommodating shell 3. Among them, the well plate 1 and the accommodating shell 3 are integrally injection-molded, and a plurality of holes are formed on the top surface of the well plate 1 corresponding to the accommodating shell 3.

[0034] In one embodiment, the water-blocking cover 2 is entirely in a rectangular structure, and the border of the water-blocking cover 2 is in contact with the side surface of the well plate 1. Through the setting of the water-blocking cover 2, it is possible to surround the end face and the side surface of the well plate 1 for one week, achieving the effect of sealing the end face of the well plate 1.

[0035] Working principle: First, use a dropper to drop the cell sample solution to be cultured into the 96 wells of the well plate 1 one by one. Then, cover and install the water-blocking cover 2 on the top surface of the well plate 1, so that several rubber plugs 4 of the water-blocking cover 2 are respectively inserted and installed into several receiving shells 3 of the well plate 1, making the 96 wells formed by several receiving shells 3 on the top surface of the well plate 1 covered and sealed. Heat and culture the well plate 1 in a 37°C water bath. When taking out the well plate 1, place the U-shaped support members 7 at both ends of the steel handle 5 under the well plate 1, so that the positioning posts 8 of the two U-shaped support members 7 are respectively inserted into the positioning sleeves 6 arranged at the four corners of the bottom surface of the well plate 1. Then, take out the well plate 1 from the water bath through the steel handle 5, and remove the water-blocking cover 2 to perform the detection work on the cell sample.

[0036] In the present utility model, unless otherwise clearly defined and limited, terms such as "installation", "setting", "connection", "fixation", "swivel connection" and the like shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two components or the interaction relationship between two components. Unless otherwise clearly defined, for those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0037] The above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A 96-well device for incubating cells in a water bath environment, comprising a well plate (1) and a water-blocking cover (2) connected to the top surface thereof, characterized in that, A plurality of receiving shells (3) are integrally formed at the bottom of the orifice plate (1), and the internal spaces of the plurality of receiving shells (3) form 96 holes. A water blocking cover (2) is mounted on the top surface of the orifice plate (1) in a covering manner, and a plurality of rubber plugs (4) are integrally formed on the inner side surface of the water blocking cover (2). The plurality of rubber plugs (4) are respectively inserted into the inner sides of the plurality of receiving shells (3). Positioning sleeves (6) are integrally formed at the four corners of the bottom surface of the orifice plate (1). A steel handle (5) is arranged above the orifice plate (1). U-shaped support members (7) are respectively welded at both ends of the steel handle (5). Positioning columns (8) are respectively welded at both ends of the U-shaped support members (7) corresponding to the positioning sleeves (6). The positioning columns (8) of the U-shaped support members (7) are inserted into the positioning sleeves (6).

2. The 96-well device for incubating cells in a water bath environment according to claim 1, wherein A plurality of rectangular protrusions are integrally injection molded on the top surface of the water blocking cover (2), and two convex rings (9) are respectively arranged on each rubber plug (4) of the water blocking cover (2).

3. The 96-well device for incubating cells in a water bath environment according to claim 1, characterized in that, A rubber sleeve (10) is sleeved in the middle of the steel handle (5), and a grid-shaped anti-slip pattern is arranged on the surface of the rubber sleeve (10).

4. The 96-well device for incubating cells in a water bath environment according to claim 1, wherein The water blocking cover (2) is entirely made of transparent silica gel, and the size of the water blocking cover (2) is adapted to the size of the orifice plate (1).

5. The 96-well device for incubating cells in a water bath environment according to claim 1, wherein The receiving shell (3) is composed of a cylindrical part and a conical part, and the cylindrical part is above the conical part. The size of the rubber plug (4) matches the size of the inner space of the cylindrical part of the receiving shell (3).

6. The 96-well device for incubating cells in a water bath environment according to claim 1, wherein, The water blocking cover (2) is entirely in a rectangular structure, and the border of the water blocking cover (2) is in contact with the side surface of the orifice plate (1).

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